WO2023286927A1 - Method for preparing plant biostimulant for preventing cold injury and enhancing flowering rate of plant - Google Patents

Abstract

A disclosed method for preparing a plant biostimulant for preventing cold injury and enhancing the flowering rate of a plant comprises the steps of: preparing an animal amino acid by hydrolyzing feather meal by means of a protease; and liquefying by mixing the prepared animal amino acid and a reaction material comprising urea, an organic acid and water. Therefore, the present invention can not only prevent cold injury of a plant and enhance the flowering rate of the plant, but also prevent agricultural damage due to climate change.

Description

Manufacturing method of plant physiological activator for preventing cold damage and improving flowering rate of plants

The present invention (Disclosure) relates to a method for producing a plant physiological activator for preventing cold damage and improving the flowering rate of plants, and specifically, by hydrolyzing proteins using proteolytic enzymes to produce animal amino acids, By mixing reactive raw materials including urea, organic acid, and water and then liquefying to prepare plant physiological activators, it is possible to prevent cold damage of plants, improve the flowering rate of plants, and prevent agricultural damage due to climate change. It relates to a method for producing a plant physiological activator for preventing cold damage and improving the flowering rate of plants that can be prevented.

Here, background art related to the present invention is provided, and they do not necessarily mean prior art (This section provides background information related to the present disclosure which is not necessarily prior art).

The world is experiencing extreme climate change due to global warming, and many agricultural damages are occurring due to El Niño, La Niño, and typhoons. damage is occurring. As a result, the flower bud is frozen, resulting in a decrease in yield and quality due to poor flowering and fertilization.

On the other hand, fertilizers used for crop cultivation are generally composed of N (nitrogen), P (phosphorus), K (potassium)-based compound fertilizers, trace element fertilizers, and organic fertilizers, and crop yields according to climate change In order to overcome the problem of reduction and quality deterioration, plant physiological activators are proposed as an alternative.

These plant physiological activators enhance the resistance to abiotic stress (drought, high/low temperature damage, cold damage, etc.) by activating the plant's physiological action, and substances (seaweed extracts, animal/vegetable amino acids, humic acids, etc.) or microorganisms.

Animal amino acids, which are used in a high proportion among them, contain several types of amino acids, such as glycine, methionine, alanine, and glutamic acid, which are obtained when proteins in animal hair and feather meal are completely hydrolyzed. It activates various physiological functions such as growth promotion, improvement of sugar content and coloration of fruit, increase in disease resistance, and stress prevention. In particular, glycine has the effect of increasing sugar content, taste improvement, and cold resistance, and proline promotes flower bud differentiation, fertilization and fruit set, Alanine is known to help improve acidity and color.

In addition, amino acids used as plant nutrients are mostly prepared by chemical hydrolysis with strong acids such as hydrochloric acid (HCl) and sulfuric acid (H ₂ SO ₄ ). Most of the chemically decomposed amino acids exist in the D form and are low in activity in organisms. Among the 18 types of amino acids, cyclic amino acids are destroyed, and when hydrochloric acid is used, they remain in the form of ammonium chloride, which is known to cause harm to crops. .

In addition, since hydrochloric acid and sulfuric acid used in the decomposition process are registered as hazardous chemicals and critically controlled substances and require very careful use, a plan to replace chemical decomposition by strong acid is needed.

On the other hand, when organic acid is foliarly sprayed on plants, it strengthens the waxy layer of the leaf to suppress the invasion of pathogens and increases resistance to pests by repelling pests, thereby protecting the leaves of fruit trees weakened by cold damage.

In addition, existing products are based on microbial preparations that utilize organic acids produced by microorganisms, but have the disadvantage of short shelf life due to low content of organic acids and weak activity of microorganisms, as well as manufacturing technology in the case of direct blending of organic acids. As a limitation of the content, it contains only a small amount of 1-3%.

As described above, as agricultural damage occurred worldwide due to abnormal climate caused by global warming, attempts were made to overcome it through the use of seed improvement, pesticides and chemical fertilizers. concept was formed.

These biostimulants refer to substances (seaweed extracts, amino acids, humic acids) or microorganisms that have the function of enhancing the resistance to abiotic stress by activating the physiological action of plants and improving the productivity and quality of harvested products. do.

Currently, biostimulant associations have been established in the United States, Europe, and Japan to push for the enactment of related laws, and in February 2020, biostimulant laws were enacted in India as well.

While interest in biostimulants is increasing worldwide, research and development on plant physiological activators is needed to overcome agricultural damage caused by climate change due to insufficient research and development in Korea.

The present invention (Disclosure) produces animal amino acids by hydrolyzing proteins using proteolytic enzymes, mixes the prepared animal amino acids with reaction raw materials including urea, organic acids and water, and then liquefies them to produce plant bioactive agents. By doing so, it is possible to prevent cold damage to plants, improve the flowering rate of plants, and provide a method for producing plant physiological activators for preventing cold damage to plants and improving flowering rates, which can prevent agricultural damage caused by climate change in advance. for the purpose of

This section provides a general summary of the disclosure and is not a comprehensive disclosure of its full scope or all of its features).

In order to solve the above problems, a method for producing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants according to any one of the various aspects described in the present invention, using a proteolytic enzyme, hydrolyzing to prepare animal amino acids; and liquefying by mixing the prepared animal amino acid with reaction raw materials including urea, organic acid, and water.

In the method for preparing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants according to one aspect of the present invention, the preparing of the animal amino acid includes: sterilizing the feather meal under high pressure; injecting the proteolytic enzyme into the feather meal; reacting the feather powder and proteolytic enzyme; Filtering after the reacting step; It may include; and freeze-drying to powderize after the filtering step.

In the method for preparing a plant physiological activator for preventing cold damage and improving the flowering rate of plants according to one aspect of the present invention, the step of high-pressure steam sterilization of the down feather meal is performed at a pressure range of 2.8-3.2 bar, 135-145 ° C. It can be performed according to the temperature range and time range of 25-35 minutes.

In the method for preparing a plant physiological activator for preventing cold damage and improving flowering rate of plants according to one aspect of the invention, the reacting step is performed according to a temperature range of 45-55 ° C and a time range of 2.5-3.5 days. can do.

In the method for preparing a plant physiologically active agent for preventing cold damage and improving flowering rate of plants according to one aspect of the invention, the filtering step may be performed according to a particle size range of 150-250 mesh.

In the method for producing a plant physiological activator for preventing cold damage and improving the flowering rate of plants according to one aspect of the invention, the step of liquefying by mixing the reaction raw materials includes the urea, organic acid and water in the animal amino acid Injecting the reaction raw material, further adding boric acid, chelate iron, sodium molybdate, zinc sulfate, and manganese sulfate; Stirring and reacting the animal amino acid and the reaction raw material; precipitating after the stirring and reacting; It may include; and filtering after the precipitating step and then aging.

In the method for producing a plant physiologically active agent for preventing cold damage and improving flowering rate of plants according to one aspect of the invention, the stirring and reacting step is a temperature range of 65-75 ° C, a time range of 3.5-4.5 hours, and It can be performed in the rotational speed range of 350-450 rpm.

In the method for preparing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants according to one aspect of the invention, the precipitating step may be performed in a time range of 22-26 hours.

In the manufacturing method of a plant physiological activator for preventing cold damage and improving flowering rate of plants according to one aspect of the invention, the step of aging after filtering is after filtering according to the particle size range of 250-350 mesh , it can be aged according to the time range of 46-50 hours.

In the method for producing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants according to one aspect of the invention, the plant physiologically active agent is diluted 900-1100 times before germination, leaf fertilization, and before flowering, respectively, by foliar fertilization. can be sprayed

According to the present invention, by hydrolyzing protein using a proteolytic enzyme to produce animal amino acids, mixing the prepared animal amino acids with reaction raw materials including urea, organic acids and water, and then liquefying to prepare plant physiologically active agents, It is possible to prevent cold damage to plants, improve the flowering rate of plants, and prevent agricultural damage due to climate change in advance.

In addition, according to the present invention, the plant physiological activator prepared by a complex technology using animal amino acids, urea, and organic acids as reaction raw materials can not only prevent cold damage to plants, but also provide physiologically active effects that improve flowering rates. there is.

1 is a flow chart showing a process for preparing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants according to an embodiment of the present invention;

2 and 3 are flowcharts showing detailed processes for preparing a plant physiologically active agent according to an embodiment of the present invention.

Hereinafter, an embodiment implementing a method for manufacturing a plant physiologically active agent for preventing cold damage and improving flowering rate of plants according to the present invention will be described in detail with reference to the drawings.

However, the essential (intrinsic) technical idea of the present invention cannot be said to be limited by the embodiments described below, and based on the essential (intrinsic) technical idea of the present invention, a person skilled in the art below It is revealed that the embodiments described in include the range that can be easily proposed as a method of substitution or change.

In addition, since the terms used below are selected for convenience of description, in grasping the essential (intrinsic) technical idea of the present invention, they are not limited to the dictionary meaning and are appropriately interpreted in a meaning consistent with the technical idea of the present invention. It should be.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a flow chart showing a process for preparing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants according to an embodiment of the present invention, and FIGS. 2 and 3 are manufacturing a plant physiologically active agent according to an embodiment of the present invention This is a flowchart showing the detailed process.

Referring to FIGS. 1 to 3 , animal amino acids may be prepared by hydrolyzing feather meal using a proteolytic enzyme (step 110).

Here, the protease is pepsin, rennin, trypsin, chymotrypsin, erepsin, enterokinase, papain, bromelin , ficin, actinidin, and elastase.

On the other hand, although the present invention has been described as hydrolyzing proteins using proteolytic enzymes, it is of course possible to hydrolyze proteins using microorganisms that produce proteolytic enzymes instead of proteolytic enzymes, for example It may include bacteria (eg, Bacillus, etc.), fungi (eg, Aspergillus, Rhizopu, etc.) and the like.

Referring to the specific process of step 110, down and feather powder can be sterilized by high-pressure steam (step 111).

Here, feather meal means that the remaining feathers after slaughtering poultry are processed using steam pressure, dried and pulverized, and have a very high crude protein of 76-87%, a very low content of crude fiber, calcium and phosphorus, and In order to improve the value, it can be used in the form of peptides by decomposing hydrogen and sulfur bonds by heating and decomposing at high temperature and high pressure.

In the step 111 of high-pressure steam sterilization of the feather powder as described above, high-pressure steam sterilization may be performed according to a pressure range of 2.8-3.2 bar, a temperature range of 135-145 ° C, and a time range of 25-35 minutes. (autoclave), the inside and outside are sealed, and the water inside the sealed container is exposed to high temperature to generate water vapor, while the pressure inside the container and the temperature are within the pressure range of 2.8-3.2 bar. After raising the temperature to 135-145 ° C., the pressure and temperature are maintained and treated according to the time range of 25-35 minutes, thereby sterilizing the bacteria present in the feather meal.

Then, the proteolytic enzyme may be added to the feather meal (step 112).

In the inputting step 112 as described above, after the high-pressure steam sterilized feather powder is introduced into the reaction vessel, a proteolytic enzyme may be added to the feather powder in the reaction vessel for hydrolysis.

Next, feather powder and proteolytic enzyme can be reacted (step 113).

In the reaction step (113) as described above, the reaction can be performed according to the temperature range of 45-55 ° C and the time range of 2.5-3.5 days. It can be reacted according to the time range of 2.5-3.5 days in the state of raising and maintaining the temperature range.

After the reacting step 113, filtering may be performed according to a preset particle size range (step 114).

In the filtering step 114 as described above, filtration can be performed according to the particle size range of 150-250 mesh. It can be filtered by passing it through a membrane filter.

After the filtering step (114), it may be freeze-dried and powdered (step 115).

In the step of pulverizing as described above (115), the filtered filtrate is frozen, the ice is removed by sublimation, and then freeze-dried in a process of removing moisture by raising the temperature, and -250 mesh, etc.) can be pulverized into powder to make it uniformly pulverized.

Next, by mixing the animal amino acid prepared in step 110 with the reaction raw material including urea, organic acid, and water to liquefy, a plant physiologically active agent for preventing cold damage and improving flowering rate of plants can be prepared (step 120). ).

Here, 30-35 parts by weight of animal amino acid and 65-70 parts by weight of a reaction raw material may be mixed based on 100 parts by weight of the entire mixture.

Describing the specific process of step 120, reaction raw materials including urea, organic acid, and water are added to animal amino acids, but boric acid, chelated iron, sodium molybdate, zinc sulfate, and manganese sulfate may be further added. (Step 121).

Here, the reaction raw materials mixed with the animal amino acid are 14-16 parts by weight of urea, 4-5 parts by weight of organic acid, 48-50 parts by weight of water, 0.5-1.0 parts by weight of boric acid, chelate iron, based on 100 parts by weight of the total mixture. 0.3 to 0.5 parts by weight, 0.01 to 0.02 parts by weight of sodium molybdate, 0.05 to 0.1 parts by weight of zinc sulfate, and 0.05 to 0.1 parts by weight of manganese sulfate.

In addition, urea contains 46% nitrogen, which makes chlorophyll that performs photosynthesis and plays a role in growing the stem, leaf, and height of crops, and constitutes enzymes, hormones, and vitamins involved in biochemical reactions in plants It is known to enhance plant growth.

In addition, organic acids include, for example, ascorbic acid, carboxylic acid, gluconic acid, succinic acid, glutamic acid, fumaric acid, acetic acid, palmitic acid, tartaric acid, lactic acid, malic acid, succinic acid, tartaric acid, citric acid, acetic acid, oxalic acid and humic acid. It includes at least one selected from the group consisting of assimilative products produced by photosynthesis through various metabolic processes, nitrogen components absorbed when fertilizing nitrogen fertilizer are combined with organic acids to be synthesized into amino acids, and suppression of diseases of various crops. It is known that it can solubilize phosphoric acid and promote phosphoric acid absorption when fertilized in soil where phosphoric acid is accumulated.

On the other hand, boric acid shows weak acidity with water-soluble boron as its main component. When the pH of the soil becomes acidic, it changes to an effective form, increasing its utilization rate, and is difficult to move in plants.

In addition, chelated iron is chelated iron, and it can suppress the effect of acidity due to electrical properties, combination with other components, and insolubilization. It is known to be effective in restoring

In addition, sodium molybdate is known to contain molybdenum and sodium to increase chlorophyll content, improve photosynthetic ability, promote plant growth and improve storage stability.

On the other hand, zinc sulfate contains sulfur and zinc, which are plant nutrients, and affects the growth and quality of crops from the beginning of crop growth. It produces auxin, a plant growth regulator, activates enzymes and synthesizes chlorophyll. is known to enhance

In addition, manganese sulfate promotes the action of oxidase and is involved in chlorophyll production and photosynthesis, nitrogen metabolism and carbon assimilation, and vitamin C formation, and is known to activate IAA oxidase to promote IAA oxidation.

Next, the animal amino acid and the reaction raw material may be stirred and reacted (step 122).

In the stirring and reaction step (122) as described above, stirring and reaction can be performed in a temperature range of 65-75 ° C, a time range of 3.5-4.5 hours, and a rotational speed range of 350-450 rpm. After being put into the stirrer, in a state where the internal temperature is raised to a temperature range of 65-75 ℃ and maintained, stirring and reacting according to the time range of 3.5-4.5 hours while rotating the stirring blades in the rotational speed range of 350-450 rpm can

After the stirring and reacting step 122, it may be precipitated (step 123).

In the precipitating step 123 as described above, precipitation may be performed according to a time range of 22-26 hours, and the reaction solution obtained through stirring and reaction is kept at room temperature (eg, 15-25 ℃) for 22-26 hours. It can be maintained for a while to precipitate solid materials remaining after stirring and reaction.

After the precipitating step (123), a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants can be prepared by filtering according to a predetermined particle size range and then maturing (step 124).

In the filtering and aging step 124, after filtering according to the particle size range of 250-350 mesh, it can be aged according to the time range of 46-50 hours, in which the remaining solid substances are precipitated. It can be filtered by passing it through a membrane filter having a particle size range of 250-350 mesh, and the filtrate is aged at room temperature (eg, 15-25 ° C.) over a time range of 46-50 hours. can

The liquid plant bioactive agent prepared as described above can be packaged and sold by required weight.

On the other hand, the plant physiological activator for preventing cold damage and improving the flowering rate of plants prepared through the process as described above is diluted 900-1100 times before germination (late March-early April), leaflet period (April Early) and before flowering (early April to mid-April), it can be sprayed with foliar fertilization, respectively. 10 ml of the prepared plant physiological activator can be mixed with about 9-11 L of water, diluted, and then sprayed.

Next, the plant physiological activator prepared as described above was diluted 1000 times before germination of plants (late March to early April), leaf growth period (early April) and before flowering (early April to mid-April). If the test results (test results of the Apple Research Institute of the National Institute of Horticultural and Herbal Science) are explained when spraying each with foliar fertilization, the effect on the cold damage and flowering rate of Hongro apple flowers is evaluated through the centralized damage rate (%) and simultaneous flowering rate (%). As a result of the observation and measurement test, as shown in Table 1 below, the plant physiological activator according to the embodiment of the present invention showed a centralization damage rate (%) of 18.8 compared to the case of spraying urea and the untreated control group, As the simultaneous flowering rate (%) was 78.8, it can be confirmed that it is effective in preventing cold damage and improving the flowering rate.

	Centering Damage Rate (%)	Simultaneous flowering rate (%)
Plant physiological activator of the present invention	18.8	78.8
Element	27.5	71.8
Control (untreated)	25.0	71.6

In addition, the test results of observing and measuring the effect on the fruit characteristics of Hongro apples through weight (g), redness, soluble solids content (ㅀBrix), fruit diameter (mm), etc., as shown in Table 2 below Likewise, the plant physiological activator according to the embodiment of the present invention showed 223.2 in weight (g) and 14.1 in redness compared to the case of spraying urea and the untreated control group, and in soluble solids content (ㅀBrix) It was 14.7, and 16.0 in fruit diameter (mm), so the fruit weight and fruit diameter were relatively smaller than in the case of urea treatment, but it was confirmed that the fruit color was clear in redness. It was confirmed that the elements appeared the same as in the case of element processing.

	Overweight (g)	redness	availability solids content (°Brix)	Diameter length (mm)
Plant physiological activator of the present invention	223.2	14.1	14.7	16.0
Element	229.1	13.3	14.7	17.0
Control (untreated)	211.3	9.9	14.3	16.7

It can be confirmed that the plant physiological activator for preventing cold damage and improving the flowering rate of plants prepared as described above contains all of the various types of amino acids contained in the amino acid powder, as shown in Table 3 below.

amino acid	Amino acid powder (umole/g)	Ectocin (umole/ml)
histidine	2.016	0.402
serine	18.441	1.837
arginine	12.117	1.107
glycine	5.432	0.573
glutamic acid	4.935	0.452
threonine	6.651	0.770
alanine	13.628	1.425
proline	5.334	0.398
cysteine	0.177	0.017
lysine	2.362	0.259
tyrosine	5.675	0.588
methionine	1.637	0.121
valine	0.999	0.002
isoleucine	2.366	0.177
leucine	4.724	0.384
phenylalanine	5.521	0.608

Therefore, according to an embodiment of the present invention, protein is hydrolyzed using a proteolytic enzyme to produce animal amino acids, and after mixing the prepared animal amino acids with reaction raw materials including urea, organic acids, and water, they are liquefied to produce plant physiology. By preparing the activator, it is possible to prevent cold damage of plants, improve the flowering rate of plants, and prevent agricultural damage due to climate change in advance.

In addition, according to an embodiment of the present invention, the plant physiological activator prepared by a complex technology using a reactive raw material containing an organic acid due to climate change can prevent damage from freezing to plants and provide a physiologically active effect that improves the flowering rate. can do.

Claims (6)

1. preparing animal amino acids by hydrolyzing feather meal using a proteolytic enzyme; and

   liquefying by mixing the prepared animal amino acid with a reaction raw material including urea, organic acid and water;

   The step of preparing the animal amino acid,

   High-pressure steam sterilization of the feather meal; injecting the proteolytic enzyme into the feather meal; reacting the feather powder and proteolytic enzyme; Filtering after the reacting step; And freeze-drying to powderize after the filtering step; Including,

   The step of high-pressure steam sterilization of the feather meal is carried out according to a pressure range of 2.8-3.2 bar, a temperature range of 135-145 ° C, and a time range of 25-35 minutes,

   The reaction step is carried out according to the temperature range of 45-55 ℃ and the time range of 2.5-3.5 days,

   The filtering step is performed according to the particle size range of 150-250 mesh,

   The step of powdering is a method for producing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants that are ground into powder of 250-350 mesh and powdered.
2. The method of claim 1,

   The step of liquefying by mixing the reaction raw materials,

   Injecting the reaction raw material including the urea, organic acid, and water into the animal amino acid, and further adding boric acid, chelate iron, sodium molybdate, zinc sulfate, and manganese sulfate;

   Stirring and reacting the animal amino acid and the reaction raw material;

   precipitating after the stirring and reacting; and

   Method for producing a plant physiologically active agent for preventing cold damage and improving the flowering rate of plants comprising; filtering after the precipitating step and then aging.
3. The method of claim 2,

   The stirring and reacting steps are performed in a temperature range of 65-75 ° C., a time range of 3.5-4.5 hours, and a rotational speed range of 350-450 rpm. Method for producing a plant physiologically active agent for preventing cold damage and improving flowering rate of plants. .
4. The method of claim 3,

   The precipitating step is a method for producing a plant physiologically active agent for preventing cold damage and improving flowering rate of plants performed according to a time range of 22-26 hours.
5. The method of claim 4,

   In the step of aging after filtering, after filtering according to the particle size range of 250-350 mesh, production of plant physiological activators for preventing cold damage and improving flowering rate of plants aged according to the time range of 46-50 hours Way.
6. The method of claim 5,

   The plant physiological activator is diluted 900-1100 times and is sprayed with foliar fertilization before germination, leaf fertilization, and flowering, respectively, to prevent cold damage and improve flowering rate of plants.

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