CN104557155A - Preparation method of insect body peptide plant physiology conditioning agent - Google Patents
Preparation method of insect body peptide plant physiology conditioning agent Download PDFInfo
- Publication number
- CN104557155A CN104557155A CN201310469372.4A CN201310469372A CN104557155A CN 104557155 A CN104557155 A CN 104557155A CN 201310469372 A CN201310469372 A CN 201310469372A CN 104557155 A CN104557155 A CN 104557155A
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- China
- Prior art keywords
- insect
- plant physiology
- polypeptide
- preparation
- amendment
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a preparation method of an insect body polypeptide plant physiology conditioning agent. The method comprises the following steps: 1, grinding insect bodies, adding the ground insect bodies into an alkaline solution with the concentration of 0.5-85%, and hydrolyzing proteins and oils of the insect bodies at 0-120DEG C to obtain a solution containing insect peptides, amino acids, active factors and fatty acids; 2, filtering the obtained solution to remove insoluble insect shells, and adding an acid to a solution obtained after the filtration in order to neutralize; and 3, filtering the insect polypeptide solution obtained in step 2 to remove insoluble substances in order to obtain the insect body polypeptide plant physiology conditioning agent. The steps of the preparation method of the insect body polypeptide plant physiology conditioning agent are simple; and the insect bodies are hydrolyzed to form peptides, active factors, amino acids and fatty acids, so the peptide plant physiology conditioning agent prepared in the invention has the characteristics of plant growth activity regulation, comprehensive amino acids, high efficiency and rich nutrition, and has an obvious fattening effect on crops.
Description
Technical field
The present invention relates to a kind of preparation method of insect body polypeptide plant physiology amendment.
Background technology
Peptide is that amino acid links together with peptide chain and the compound that formed, and peptide is also the intermediate product of proteolysis simultaneously.The peptide of 10 to 50 amino acid compositions is called polypeptide.Polypeptide has biologically active polypeptides and artificial synthetic polypeptide two kinds.The polypeptide of biological extraction has very strong activity, so be called active polypeptide.The growth of bioactive peptide major control animal and plant body, growth, immunomodulatory and metabolism, it is in a kind of equilibrium state in animal and plant body.Active polypeptide not only has trophism, its amino acid can absorb by animal and plant body; And active polypeptide also has and regulates the growth of animal and plant body, it is usually as growth regulator, promotes the growth of animal and plant body, to the growth of plant particularly photosynthesis there is unique promoter action.Polypeptide directly can be absorbed by plant, for farm crop, has the effect of volume increase, sterilizing, desinsection, raising fruit quality.
Polypeptide amendment is the promotive factor and foliar nutrient that are absorbed by plant leaf, has promote efficiently and fattening effect the growth of plant.The blade of plant has upper and lower two-layer epidermis; be made up of epidermic cell; there are stratum corneum and wax in the outside of upper epidermis cell; the functions such as the enforcement of the mesophyll cell under face tissue is photosynthetic, breathing can be protected; not by the impact of extraneous unfavourable condition change; blade performance also has many small pores, exercises the function that gas is changed.Stratum corneum is made up of a kind of Long carbon chain fatty acid polymer with hydroxyl and carboxyl, hydroxyl on the molecular gap of this polymkeric substance and molecule, carboxylated hydrophilic group can allow the aqueous solution penetrate in leaf, and the pore of blade surface is that polypeptide amendment enters blade passage more easily.
The foliage fertilizer that Vehicles Collected from Market is sold is amino acid foliage fertilizer mostly, and obtain prepared by the many tankage produced by glutamate production and leather of amino acid foliage fertilizer, its amino acid composition is not comprehensive; kind is few; active low, and be often mixed with industrial pollutants, sometimes harm is produced to the growth of plant.Further, current amino acid foliage fertilizer containing active bio polypeptide fraction, does not have the active factor of growth promoting effects.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of preparation method of insect body polypeptide plant physiology amendment, preparation process is simple, and the obtained growth of plant physiology amendment to farm crop has obvious fattening effect.
The preparation method of insect body polypeptide plant physiology amendment of the present invention, comprises the following steps:
1) ground by insect body, joining concentration is in the alkali lye of 0.5-85%, at 0-120
obe hydrolyzed protein and the grease of insect body at the temperature of C, obtain the solution containing insect polypeptide, amino acid, active factor and lipid acid;
2) solution obtained above is filtered, remove insoluble insect shell, in the solution that filtration obtains, add acid, neutralize;
3) will through step 2) the insect polypeptide solution that obtains filters, and removing insolubles, obtains insect polypeptide plant physiology amendment product.
The preparation method of insect body polypeptide plant physiology amendment of the present invention, in step 1): the mass ratio of described insect body and alkali lye is 1:8-100.
The preparation method of insect body polypeptide plant physiology amendment of the present invention, in step 2) in: the pH value after neutralizing is 5-8.
The preparation method of insect body polypeptide plant physiology amendment of the present invention, the amendment after described preparation is applicable to various plants, has good fattening, somatotrophic effect; Described plant is field-crop, vegetables, fruit tree, lawn, flowers, potted plant or greenhouse cultivation thing.
Compared with prior art beneficial effect of the present invention is: preparation method's step of insect body polypeptide plant physiology amendment of the present invention is simple, adopt insect body to be hydrolyzed to polypeptide, active factor, amino acid and lipid acid, the polypeptide plant physiology amendment prepared has coordinate plant growth activity, comprehensive, efficient, the contained nutritious feature of amino acid classes; Polypeptide plant physiology amendment is rich in various trace elements and nutritive substance, has obvious fattening effect to the growth of farm crop, and simultaneously insect body is rich in multiple proteins, active factor and high-quality insect oil and multivitamin and trace element; The wide material sources of insect, can be got by agricultural pests, also can derive from artificial breeding, and the insect polypeptide plant physiology amendment be made up of insect body has environmental protection, economic feature.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
A preparation method for insect body polypeptide plant physiology amendment, comprises the following steps, and weighs 30 grams of bollworms, joins in saturated ammoniacal liquor (ammonium hydroxide) solution of 300 milliliters, 70
oconfined reaction is hydrolyzed 3 hours at the temperature of C.Filtered by solution after hydrolysis, remove insoluble worm skin, in the solution after filtration, add the acetic acid of 65 milliliters, being neutralized to pH value is 7, filters above-mentioned solution, obtains insect polypeptide plant physiology amendment product.
Embodiment 2
A preparation method for insect body polypeptide plant physiology amendment, comprises the following steps, and weighs 50 grams of bollworms, joins in saturated ammoniacal liquor (ammonium hydroxide) solution of 800 milliliters, 70
oconfined reaction is hydrolyzed 5 hours at the temperature of C.Filtered by solution after hydrolysis, remove insoluble worm skin, in the solution after filtration, add the acetic acid of 85 milliliters, being neutralized to pH value is 7, filters above-mentioned solution, obtains insect polypeptide plant physiology amendment product.
Embodiment 3
A preparation method for insect body polypeptide plant physiology amendment, comprises the following steps, and weighs 60 grams of bollworms, joins in saturated ammoniacal liquor (ammonium hydroxide) solution of 1600 milliliters, 70
oconfined reaction is hydrolyzed 7 hours at the temperature of C.Filtered by solution after hydrolysis, remove insoluble worm skin, in the solution after filtration, add the acetic acid of 95 milliliters, being neutralized to pH value is 7, filters above-mentioned solution, obtains insect polypeptide plant physiology amendment product.
The insect body polypeptide plant physiology amendment of employing prepared by the present invention, when specifically using, dilutes 600 times to water, is sprayed on the green vegetables of field crop Shanghai.Not spray the field crop Shanghai green vegetables of foliage fertilizer in contrast.Observed every 2 days.Found that, spraying has the field crop of the above-mentioned amendment prepared by insect body obvious compared with blank group blade face greener, growth young leaves longer, spray have the blade length of insect polypeptide comparatively blank group within one week, increase to 20% to 30%, newborn blade is straight and upright.Field test shows that the amendment prepared by the present invention has obvious fattening effect.
Insect body polypeptide plant physiology amendment prepared by the present invention is placed 2 months at ambient temperature simultaneously, do not add any sanitas, not spoiled, do not foul, visual inspection does not have corrupt mushroom material simultaneously.The insect polypeptide plant physiology amendment that this obtains contains germ resistance active polypeptide composition, stable performance.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvement and modification, these improve and modification also should be considered as protection scope of the present invention.
Claims (4)
1. a preparation method for insect body polypeptide plant physiology amendment, is characterized in that, comprise the following steps:
1) ground by insect body, joining concentration is in the alkali lye of 0.5-85%, at 0-120
obe hydrolyzed protein and the grease of insect body at the temperature of C, obtain the solution containing insect polypeptide, amino acid, active factor and lipid acid;
2) solution obtained above is filtered, remove insoluble insect shell, in the solution that filtration obtains, add acid, neutralize;
3) will through step 2) the insect polypeptide solution that obtains filters, and removing insolubles, obtains insect polypeptide plant physiology amendment product.
2. the preparation method of insect body polypeptide plant physiology amendment according to claim 1, is characterized in that, in step 1): the mass ratio of described insect body and alkali lye is 1:8-100.
3. the preparation method of insect body polypeptide plant physiology amendment according to claim 1, is characterized in that, in step 2) in: the pH value after neutralizing is 5-8.
4. the preparation method of insect body polypeptide plant physiology amendment according to claim 1, it is characterized in that, the amendment after described preparation is applicable to various plants, has good fattening, somatotrophic effect; Described plant is field-crop, vegetables, fruit tree, lawn, flowers, potted plant or greenhouse cultivation thing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310469372.4A CN104557155A (en) | 2013-10-10 | 2013-10-10 | Preparation method of insect body peptide plant physiology conditioning agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310469372.4A CN104557155A (en) | 2013-10-10 | 2013-10-10 | Preparation method of insect body peptide plant physiology conditioning agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104557155A true CN104557155A (en) | 2015-04-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310469372.4A Pending CN104557155A (en) | 2013-10-10 | 2013-10-10 | Preparation method of insect body peptide plant physiology conditioning agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110590411A (en) * | 2019-10-21 | 2019-12-20 | 武汉楚强生物科技有限公司 | Amino acid hydrolysate based on insect residues with viruses and preparation method and application thereof |
| CN110683913A (en) * | 2019-10-21 | 2020-01-14 | 武汉楚强生物科技有限公司 | Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502590A (en) * | 2002-11-25 | 2004-06-09 | 中国海洋大学 | Technology for preparing fertilizer by using Brine shrimp |
| CN1785918A (en) * | 2004-12-06 | 2006-06-14 | 邵建华 | Production of amino acid foliage fertilizer using weste crustacean and its crust |
| CN101481266A (en) * | 2009-02-13 | 2009-07-15 | 杜振宁 | Plant nutrient agent, preparation and use |
-
2013
- 2013-10-10 CN CN201310469372.4A patent/CN104557155A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502590A (en) * | 2002-11-25 | 2004-06-09 | 中国海洋大学 | Technology for preparing fertilizer by using Brine shrimp |
| CN1785918A (en) * | 2004-12-06 | 2006-06-14 | 邵建华 | Production of amino acid foliage fertilizer using weste crustacean and its crust |
| CN101481266A (en) * | 2009-02-13 | 2009-07-15 | 杜振宁 | Plant nutrient agent, preparation and use |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110590411A (en) * | 2019-10-21 | 2019-12-20 | 武汉楚强生物科技有限公司 | Amino acid hydrolysate based on insect residues with viruses and preparation method and application thereof |
| CN110683913A (en) * | 2019-10-21 | 2020-01-14 | 武汉楚强生物科技有限公司 | Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof |
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Application publication date: 20150429 |
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