CN113439087A - 重组蛋白的制备方法 - Google Patents

重组蛋白的制备方法 Download PDF

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Publication number
CN113439087A
CN113439087A CN202080011556.4A CN202080011556A CN113439087A CN 113439087 A CN113439087 A CN 113439087A CN 202080011556 A CN202080011556 A CN 202080011556A CN 113439087 A CN113439087 A CN 113439087A
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CN
China
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protein
cell
recombinant
gly
ser
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CN202080011556.4A
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English (en)
Chinese (zh)
Inventor
中东宪治
蒋李
野田昂文
津山研二
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Kran Corp.
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Spiber Inc
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Publication of CN113439087A publication Critical patent/CN113439087A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080011556.4A 2019-01-31 2020-01-30 重组蛋白的制备方法 Pending CN113439087A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2019015716 2019-01-31
JP2019-015716 2019-01-31
JP2019-142388 2019-08-01
JP2019142388 2019-08-01
PCT/JP2020/003487 WO2020158877A1 (ja) 2019-01-31 2020-01-30 組換えタンパク質の製造方法

Publications (1)

Publication Number Publication Date
CN113439087A true CN113439087A (zh) 2021-09-24

Family

ID=71840352

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080011556.4A Pending CN113439087A (zh) 2019-01-31 2020-01-30 重组蛋白的制备方法

Country Status (8)

Country Link
US (1) US20220033870A1 (https=)
EP (1) EP3919506A4 (https=)
JP (2) JP6968468B2 (https=)
CN (1) CN113439087A (https=)
BR (1) BR112021014805A2 (https=)
CA (1) CA3127177A1 (https=)
SG (1) SG11202107697UA (https=)
WO (1) WO2020158877A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021020546A1 (ja) * 2019-08-01 2021-02-04 Ｓｐｉｂｅｒ株式会社 組換え細胞及び組換えタンパク質の製造方法
MX2024010846A (es) * 2022-03-24 2024-09-11 Richter Gedeon Nyrt Metodo para la preparacion de biofarmacos.

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WO2000044906A2 (en) * 1999-01-27 2000-08-03 Elitra Pharmaceuticals, Inc. GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI)
CN1639333A (zh) * 2002-03-06 2005-07-13 东丽株式会社 利用基因重组蚕生产生理活性蛋白质的方法
CN101605887A (zh) * 2006-05-16 2009-12-16 协和发酵麒麟株式会社 蛋白的高分泌生产方法
CN105331568A (zh) * 2014-06-12 2016-02-17 清华大学 一种通过增大细菌体积而增加微生物胞内内含物积累量的方法
WO2018025886A1 (ja) * 2016-08-02 2018-02-08 Ｓｐｉｂｅｒ株式会社 組換えタンパク質の生産方法
CN108218972A (zh) * 2007-03-16 2018-06-29 巴西农牧研究企业 来自Nephilengys Cruentata蜘蛛、黄带粉趾食鸟蛛和Parawixia Bistriata蜘蛛的网的蛋白

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JPS58110600A (ja) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JPS60221091A (ja) 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
JP2564268B2 (ja) 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
EP0326046A3 (en) 1988-01-25 1990-06-13 Takeda Chemical Industries, Ltd. Production of human epidermal growth factor
JPH0322979A (ja) 1989-06-19 1991-01-31 Kyowa Hakko Kogyo Co Ltd 新規プラスミノーゲン活性化因子
JP3012026B2 (ja) 1991-03-05 2000-02-21 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクター を保有する微生物並びに該微生物を 用いる有用物質の製造法
JP3433807B2 (ja) 1992-03-31 2003-08-04 鵜高 重三 新規アミノ酸配列及び該アミノ酸配列をコードするｄｎａを保有する発現ベクターを組み込んだバチルス・ブレビスを用いる有用物質の製造法
JP2727391B2 (ja) 1992-07-23 1998-03-11 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクターを保有する微生物並びに該微生物を用いる有用物質の製造法
JP2771756B2 (ja) 1993-04-09 1998-07-02 東洋エンジニアリング株式会社 組換え大腸菌の培養方法
JP3753945B2 (ja) 2001-02-14 2006-03-08 ヒゲタ醤油株式会社 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター
KR100499647B1 (ko) * 2002-09-25 2005-07-05 한국과학기술원 박테리아에서의 외래단백질의 생산성을 증가시키는 방법
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* Cited by examiner, † Cited by third party
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WO2000044906A2 (en) * 1999-01-27 2000-08-03 Elitra Pharmaceuticals, Inc. GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI)
CN1639333A (zh) * 2002-03-06 2005-07-13 东丽株式会社 利用基因重组蚕生产生理活性蛋白质的方法
CN101605887A (zh) * 2006-05-16 2009-12-16 协和发酵麒麟株式会社 蛋白的高分泌生产方法
CN108218972A (zh) * 2007-03-16 2018-06-29 巴西农牧研究企业 来自Nephilengys Cruentata蜘蛛、黄带粉趾食鸟蛛和Parawixia Bistriata蜘蛛的网的蛋白
CN105331568A (zh) * 2014-06-12 2016-02-17 清华大学 一种通过增大细菌体积而增加微生物胞内内含物积累量的方法
WO2018025886A1 (ja) * 2016-08-02 2018-02-08 Ｓｐｉｂｅｒ株式会社 組換えタンパク質の生産方法

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BENJAMIN BANUSCH: "Biophysical Studies of the Effect of MreB Mutants on Cell Stiffness in Escherischa Coli", MASTER THESIS IN NANOSCIENCE, vol. 23, no. 2, 30 November 2013 (2013-11-30), pages 1 - 40 *
HISAKO MASUDA等: "YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli", MOLECULAR MICROBIOLOGY, vol. 84, no. 5, pages 979 - 989, XP055728104, DOI: 10.1111/j.1365-2958.2012.08068.x *
XIAO-RAN JIANG等: "Controlling cell volume for efficient PHB production by Halomonas", METABOLIC ENGINEERING, vol. 44, no. 12, pages 30 - 37, XP085285796, DOI: 10.1016/j.ymben.2017.09.004 *
XIAO-RAN JIANG等: "Morphology engineering of bacteria for bio-production", BIOTECHNOLOGY ADVANCES, vol. 34, no. 4, pages 435 - 440, XP029549277, DOI: 10.1016/j.biotechadv.2015.12.007 *
周丹霞等: "蜜蜂螺原体细胞骨架相关基因mreB的克隆表达及在螺原体二种形态时的表达差异", 微生物学通报, vol. 41, no. 9, 20 September 2014 (2014-09-20), pages 1771 - 1778 *

Also Published As

Publication number Publication date
JP2021101744A (ja) 2021-07-15
EP3919506A1 (en) 2021-12-08
JP6968468B2 (ja) 2021-11-17
BR112021014805A2 (pt) 2022-01-04
US20220033870A1 (en) 2022-02-03
WO2020158877A1 (ja) 2020-08-06
SG11202107697UA (en) 2021-08-30
CA3127177A1 (en) 2020-08-06
EP3919506A4 (en) 2022-11-02
JP7526489B2 (ja) 2024-08-01
JPWO2020158877A1 (ja) 2021-02-18

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Address after: Yamagata Prefecture, Japan

Applicant after: Kran Corp.

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Address before: Kazuoka City, Yamagata Prefecture, Japan

Applicant before: SPIBER Inc.

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