EP3919506A4 - METHODS FOR PRODUCTION OF RECOMBINANT PROTEINS - Google Patents

METHODS FOR PRODUCTION OF RECOMBINANT PROTEINS Download PDF

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Publication number
EP3919506A4
EP3919506A4 EP20749538.3A EP20749538A EP3919506A4 EP 3919506 A4 EP3919506 A4 EP 3919506A4 EP 20749538 A EP20749538 A EP 20749538A EP 3919506 A4 EP3919506 A4 EP 3919506A4
Authority
EP
European Patent Office
Prior art keywords
producing
recombination protein
recombination
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20749538.3A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP3919506A1 (en
Inventor
Kenji Nakahigashi
Li Jiang
Takanobu Noda
Kenji Tsuyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Inc
Original Assignee
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Inc filed Critical Spiber Inc
Publication of EP3919506A1 publication Critical patent/EP3919506A1/en
Publication of EP3919506A4 publication Critical patent/EP3919506A4/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP20749538.3A 2019-01-31 2020-01-30 METHODS FOR PRODUCTION OF RECOMBINANT PROTEINS Pending EP3919506A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019015716 2019-01-31
JP2019142388 2019-08-01
PCT/JP2020/003487 WO2020158877A1 (ja) 2019-01-31 2020-01-30 組換えタンパク質の製造方法

Publications (2)

Publication Number Publication Date
EP3919506A1 EP3919506A1 (en) 2021-12-08
EP3919506A4 true EP3919506A4 (en) 2022-11-02

Family

ID=71840352

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20749538.3A Pending EP3919506A4 (en) 2019-01-31 2020-01-30 METHODS FOR PRODUCTION OF RECOMBINANT PROTEINS

Country Status (8)

Country Link
US (1) US20220033870A1 (https=)
EP (1) EP3919506A4 (https=)
JP (2) JP6968468B2 (https=)
CN (1) CN113439087A (https=)
BR (1) BR112021014805A2 (https=)
CA (1) CA3127177A1 (https=)
SG (1) SG11202107697UA (https=)
WO (1) WO2020158877A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021020546A1 (ja) * 2019-08-01 2021-02-04 Ｓｐｉｂｅｒ株式会社 組換え細胞及び組換えタンパク質の製造方法
MX2024010846A (es) * 2022-03-24 2024-09-11 Richter Gedeon Nyrt Metodo para la preparacion de biofarmacos.

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JPS58110600A (ja) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JPS60221091A (ja) 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
JP2564268B2 (ja) 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
EP0326046A3 (en) 1988-01-25 1990-06-13 Takeda Chemical Industries, Ltd. Production of human epidermal growth factor
JPH0322979A (ja) 1989-06-19 1991-01-31 Kyowa Hakko Kogyo Co Ltd 新規プラスミノーゲン活性化因子
JP3012026B2 (ja) 1991-03-05 2000-02-21 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクター を保有する微生物並びに該微生物を 用いる有用物質の製造法
JP3433807B2 (ja) 1992-03-31 2003-08-04 鵜高 重三 新規アミノ酸配列及び該アミノ酸配列をコードするｄｎａを保有する発現ベクターを組み込んだバチルス・ブレビスを用いる有用物質の製造法
JP2727391B2 (ja) 1992-07-23 1998-03-11 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクターを保有する微生物並びに該微生物を用いる有用物質の製造法
JP2771756B2 (ja) 1993-04-09 1998-07-02 東洋エンジニアリング株式会社 組換え大腸菌の培養方法
AU3584500A (en) * 1999-01-27 2000-08-18 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in escherichia coli
JP3753945B2 (ja) 2001-02-14 2006-03-08 ヒゲタ醤油株式会社 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター
CN101712955B (zh) * 2002-03-06 2013-06-05 东丽株式会社 利用基因重组蚕生产生理活性蛋白质的方法
KR100499647B1 (ko) * 2002-09-25 2005-07-05 한국과학기술원 박테리아에서의 외래단백질의 생산성을 증가시키는 방법
KR20090031359A (ko) * 2006-05-16 2009-03-25 교와 핫꼬 기린 가부시키가이샤 단백질의 고분비 생산 방법
BRPI0701826B1 (pt) * 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
EP3147369B1 (en) * 2014-05-21 2025-08-06 Ajinomoto Co., Inc. Fibroin-like protein production method
CN105331568B (zh) * 2014-06-12 2019-01-04 清华大学 一种通过增大细菌体积而增加微生物胞内内含物积累量的方法
JP7452830B2 (ja) * 2016-08-02 2024-03-19 Ｓｐｉｂｅｒ株式会社 組換えタンパク質の生産方法

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
JIANG XIAO-RAN ET AL: "Controlling cell volume for efficient PHB production byHalomonas", METABOLIC ENGINEERING, vol. 44, November 2017 (2017-11-01), pages 30 - 37, XP085285796, ISSN: 1096-7176, DOI: 10.1016/J.YMBEN.2017.09.004 *
JIANG XIAO-RAN ET AL: "Engineering the bacterial shapes for enhanced inclusion bodies accumulation", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 29, 11 April 2015 (2015-04-11), pages 227 - 237, XP029590706, ISSN: 1096-7176, DOI: 10.1016/J.YMBEN.2015.03.017 *
JIANG XIAO-RAN ET AL: "Morphology engineering of bacteria for bio-production", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 34, no. 4, 19 December 2015 (2015-12-19), pages 435 - 440, XP029549277, ISSN: 0734-9750, DOI: 10.1016/J.BIOTECHADV.2015.12.007 *
MONDS RUSSELL D. ET AL: "Systematic Perturbation of Cytoskeletal Function Reveals a Linear Scaling Relationship between Cell Geometry and Fitness", CELL REPORTS, vol. 9, no. 4, 1 November 2014 (2014-11-01), US, pages 1528 - 1537, XP055960920, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2014.10.040 *
See also references of WO2020158877A1 *
SHI HANDUO ET AL: "Deep Phenotypic Mapping of Bacterial Cytoskeletal Mutants Reveals Physiological Robustness to Cell Size", CURRENT BIOLOGY, vol. 27, no. 22, 20 November 2017 (2017-11-20), pages 3419, XP085269020, ISSN: 0960-9822, DOI: 10.1016/J.CUB.2017.09.065 *
SHI HANDUO ET AL: "How to Build a Bacterial Cell: MreB as the Foreman of E. coli Construction", CELL, vol. 172, no. 6, 1 March 2018 (2018-03-01), Amsterdam NL, pages 1294 - 1305, XP055960918, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.02.050 *

Also Published As

Publication number Publication date
JP2021101744A (ja) 2021-07-15
EP3919506A1 (en) 2021-12-08
CN113439087A (zh) 2021-09-24
JP6968468B2 (ja) 2021-11-17
BR112021014805A2 (pt) 2022-01-04
US20220033870A1 (en) 2022-02-03
WO2020158877A1 (ja) 2020-08-06
SG11202107697UA (en) 2021-08-30
CA3127177A1 (en) 2020-08-06
JP7526489B2 (ja) 2024-08-01
JPWO2020158877A1 (ja) 2021-02-18

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