CN113248305A - Carbon-based compound enzyme fertilizer and preparation method thereof - Google Patents
Carbon-based compound enzyme fertilizer and preparation method thereof Download PDFInfo
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- CN113248305A CN113248305A CN202110534597.8A CN202110534597A CN113248305A CN 113248305 A CN113248305 A CN 113248305A CN 202110534597 A CN202110534597 A CN 202110534597A CN 113248305 A CN113248305 A CN 113248305A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a carbon-based compound enzyme fertilizer which comprises the following raw materials in parts by weight: 1-80 parts of agricultural, forestry and livestock waste fermentation liquor or extract, 0.1-10 parts of humic acid, 1-20 parts of amylase, 2-35 parts of invertase, 1-15 parts of protease, 3-30 parts of bacillus subtilis, 4-35 parts of yeast, 0.1-10 parts of fertilizer containing carbon, nitrogen, phosphorus, potassium, sulfur, calcium and magnesium elements, 0.1-5 parts of fertilizer containing iron, manganese, zinc, boron and rare earth elements and 10-35 parts of multi-source plant complex enzyme. The invention solves the problems of the persistence of the activity of the enzyme fertilizer, the comprehensiveness of nutrients and the growth of crops in adverse environments (such as saline-alkali soil and the like), improves the fertilizer efficiency, activates the fertility in the soil and reduces the using amount of chemical fertilizers.
Description
Technical Field
The invention belongs to the technical field of fertilizer production, and particularly relates to a carbon-based compound enzyme fertilizer and a preparation method thereof.
Background
The enzyme fertilizer is divided into two types, one is a high-purity water-soluble organic enzyme fertilizer which is added with any functional enzyme extracted from plants and has the functions of enhancing plant photosynthesis and promoting plants to self-prepare and meet the requirements of healthy growth, and the other is an enzyme fertilizer which is added with specific enzymes such as amylase or antioxidant dismutase and the like, and the enzyme fertilizer can not meet the requirements of tens of millions of enzymes needed by plant growth.
At present, the prior art does not solve the comprehensive problem of enzyme fertilizer nutrients, the prior enzyme fertilizer has single nutrition, the enzyme fertilizer activity retention time is short, and the problem of crop growth under adverse environment (such as saline-alkali soil) is difficult to solve.
Therefore, the development of a carbon-based compound enzyme fertilizer with long activity retention time and relatively comprehensive nutrient components and a preparation method thereof are problems to be solved by technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a carbon-based compound enzyme fertilizer with long enzyme fertilizer activity retention time and comprehensive nutrient components and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a carbon-based compound enzyme fertilizer comprises the following raw materials in parts by weight: 1-80 parts of agricultural, forestry and livestock waste fermentation liquor or extract, 0.1-10 parts of humic acid, 1-20 parts of amylase, 2-35 parts of invertase, 1-15 parts of protease, 3-30 parts of bacillus subtilis, 4-35 parts of yeast, 0.1-10 parts of fertilizer containing carbon, nitrogen, phosphorus, potassium, sulfur, calcium and magnesium elements, 0.1-5 parts of fertilizer containing iron, manganese, zinc, boron and rare earth elements and 10-35 parts of multi-source plant complex enzyme.
The invention has the beneficial effects that: the invention solves the problems of the durability of the activity of the enzyme fertilizer, the comprehensive performance of nutrients and the growth of crops in adverse environments (such as saline-alkali soil and the like), improves the fertilizer efficiency, activates the fertility in soil and reduces the using amount of the fertilizer.
Further, the amylase is alpha-amylase or beta-amylase.
Adopt above-mentioned further beneficial effect: the amylase of the invention provides nutrition and energy for crops.
Further, the invertase is a vacuolar sucrase and/or a cytoplasmic sucrase.
Adopt above-mentioned further beneficial effect: the invertase of the invention enhances crop osmotic regulation ability and stress-resistant growth and reproduction.
Further, the protease is one or a mixture of serine protease, thiol protease or metallo protease.
Adopt above-mentioned further beneficial effect: the protease of the present invention decomposes proteins to supply a carbon source to yeast, thereby promoting fermentation. The fermentation quality of the enzyme fertilizer is ensured.
Further, the fertilizer containing the carbon elements of nitrogen, phosphorus, potassium, sulfur, calcium and magnesium is a mixture of calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and magnesium sulfate.
Adopt above-mentioned further beneficial effect: the fertilizer containing carbon, nitrogen, phosphorus, potassium, sulfur, calcium and magnesium elements increases the main fertilizer components of the nutrition of the enzyme fertilizer and simultaneously plays the role of an activator.
Further, the fertilizer containing the iron, manganese, zinc and boron rare earth elements is a mixture of ferrous sulfate, manganese sulfate, zinc sulfate, boric acid and mixed rare earth nitrate.
Adopt above-mentioned further beneficial effect: the fertilizer containing the iron, manganese, zinc, boron and rare earth elements fully supplements other necessary medium and trace elements in the nutrition of the enzyme fertilizer and simultaneously plays a role of an activator.
The invention also provides a preparation method of the carbon-based compound enzyme fertilizer, which comprises the following steps:
(1) weighing the raw materials according to the carbon-based compound enzyme fertilizer;
(2) mixing and heating agricultural and forestry animal waste fermentation liquor or extract, humic acid, amylase, invertase, protease, bacillus subtilis, saccharomycetes, a chemical fertilizer containing carbon, nitrogen, phosphorus, potassium, sulfur, calcium and magnesium elements, a chemical fertilizer containing iron, manganese, zinc, boron and rare earth elements and a multi-source plant complex enzyme to 30 ℃, fermenting for 10-15 days at constant temperature, and cooling to room temperature to obtain the carbon-based complex enzyme fertilizer.
The invention has the beneficial effects that: the carbon-based compound enzyme fertilizer has simple production process, good stability and convenient industrial production.
Further, the preparation method of the fermentation liquor or the extracting solution of the agricultural, forestry and livestock wastes comprises the following steps: crushing the agricultural and forestry wastes, mixing the crushed agricultural and forestry wastes with livestock excrements, adding a leavening agent, stirring, pouring the mixture into a fermentation tank, covering the fermentation tank with a plastic film, fermenting at normal temperature, and performing dry-wet separation after three months to obtain a liquid part, namely agricultural and forestry and livestock waste fermentation liquor;
or crushing the agricultural and forestry waste and then carrying out dry distillation to obtain the wood vinegar, namely the agricultural and forestry and animal waste extracting solution.
Adopt above-mentioned further beneficial effect: agricultural and forestry wastes and livestock excrements are fully utilized, the raw material sources are rich and various, the environment is protected, pollution is avoided, and the circular agriculture with sustainable development is realized.
Further, the preparation method of the multi-source plant complex enzyme comprises the following steps: mechanically crushing rice, fructus Lycii, fructus Mali Pumilae, fructus Actinidiae chinensis, and semen Maydis, and extracting at room temperature by dilute salt method.
Adopt above-mentioned further beneficial effect: the enzyme fertilizer has cellular enzymes of various crops with outstanding characteristics, and is subjected to targeted reasonable compatibility fermentation, so that the yield and quality improvement of the enzyme fertilizer on the crop pairs and the stress resistance characteristics of salt and alkali resistance, disease resistance, cold resistance, drought resistance and the like are more outstanding and obvious.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
The preparation methods of the agricultural and forestry animal waste fermentation liquid, the agricultural and forestry animal waste extracting solution and the multi-source plant complex enzyme used in the embodiments 1 to 3 are as follows:
(1) preparing agricultural, forestry and livestock waste fermentation liquor: crushing plant straw waste into particles with the particle size of less than or equal to 0.5 cm, mixing the particles with cattle, sheep and pig excreta, adding a leavening agent, uniformly stirring, pouring the mixture into a fermentation tank, covering the fermentation tank with a plastic film, fermenting at normal temperature, performing dry-wet separation after three months, placing a submersible pump at the bottom of the fermentation tank to pump out liquid, namely, agriculture, forestry and livestock waste fermentation liquid, and wrapping a pump body with a 35-mesh screen.
(2) Preparing an agricultural, forestry and livestock waste extracting solution: crushing the plant straw waste into particles with the particle size of less than or equal to 0.5 cm, and performing dry distillation at 350 ℃ under normal pressure to obtain the wood vinegar which is the agricultural, forestry and livestock waste extracting solution.
(3) Preparing multi-source plant complex enzyme: mechanically crushing rice, fructus Lycii, fructus Mali Pumilae, fructus Actinidiae chinensis, and semen Maydis to particle size of 0.5 cm or less, and extracting at room temperature with dilute salt method.
Example 1
The preparation method of the carbon-based compound enzyme fertilizer comprises the following steps:
(1) weighing 10kg of agricultural, forestry and livestock waste fermentation liquor, 0.15kg of humic acid, 1kg of beta-amylase, 1kg of each of vacuolar sucrase and cytoplasmic sucrase, 1kg of each of serine protease and metallo protease, 3kg of bacillus subtilis, 4kg of yeast, 0.03kg of calcium nitrate, 0.07kg of potassium nitrate, 0.05kg of diammonium hydrogen phosphate, 0.1kg of magnesium sulfate, 0.03kg of each of ferrous sulfate, manganese sulfate, zinc sulfate, boric acid and mixed rare earth nitrate, and 10kg of multi-source plant complex enzyme;
(2) mixing and heating agricultural and forestry animal waste fermentation liquor, humic acid, beta-amylase, vacuole type invertase, cytoplast type invertase, serine protease, metal protease, bacillus subtilis, saccharomycetes, calcium nitrate, potassium nitrate, diammonium phosphate, magnesium sulfate, ferrous sulfate, manganese sulfate, zinc sulfate, boric acid, mixed rare earth nitrate and multi-source plant complex enzyme to 30 ℃, fermenting at constant temperature for 10 days, and cooling to room temperature to obtain the carbon-based complex enzyme fertilizer.
Example 2
The preparation method of the carbon-based compound enzyme fertilizer comprises the following steps:
(1) weighing 40kg of agricultural, forestry and livestock waste fermentation liquor, 0.8kg of humic acid, 10kg of alpha-amylase, 9kg of each of vacuolar sucrase and cytoplasmic sucrase, 7kg of metalloproteinase, 18kg of bacillus subtilis, 22kg of yeast, 0.15kg of calcium nitrate, 0.25kg of potassium nitrate, 0.18kg of diammonium hydrogen phosphate, 0.35kg of magnesium sulfate, 0.1kg of each of ferrous sulfate, manganese sulfate, zinc sulfate and boric acid, 0.18kg of mixed rare earth nitrate and 23kg of multi-source plant complex enzyme;
(2) mixing and heating agricultural, forestry and livestock waste fermentation liquor, humic acid, alpha-amylase, vacuole type invertase, cytoplast type invertase, metalloproteinase, bacillus subtilis, saccharomycetes, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate, magnesium sulfate, ferrous sulfate, manganese sulfate, zinc sulfate, boric acid, mixed rare earth nitrate and a multi-source plant complex enzyme to 30 ℃, fermenting at constant temperature for 12 days, and cooling to room temperature to obtain the carbon-based complex enzyme fertilizer.
Example 3
The preparation method of the carbon-based compound enzyme fertilizer comprises the following steps:
(1) weighing 80kg of agricultural, forestry and livestock waste extracting solution, 1kg of humic acid, 20kg of alpha-amylase, 35kg of liquid cell type sucrase, 15kg of serine protease, 30kg of bacillus subtilis, 35kg of saccharomycetes, 0.25kg of calcium nitrate, 0.6kg of potassium nitrate, 0.45kg of diammonium hydrogen phosphate, 0.7kg of magnesium sulfate, 0.25kg of ferrous sulfate, 0.4kg of manganese sulfate, 0.4kg of zinc sulfate and 0.4kg of mixed rare earth nitrate and 35kg of multi-source plant composite enzyme;
(2) mixing and heating agricultural and forestry animal waste extract, humic acid, alpha-amylase, vacuole sucrase, serine protease, bacillus subtilis, saccharomycetes, calcium nitrate, potassium nitrate, diammonium phosphate, magnesium sulfate, ferrous sulfate, manganese sulfate, zinc sulfate, boric acid, mixed rare earth nitrate and multi-source plant complex enzyme to 30 ℃, fermenting for 15 days at constant temperature, and cooling to room temperature to obtain the carbon-based complex enzyme fertilizer.
Effect test
1. The influence of the carbon-based compound enzyme fertilizer on the germination rate of rice seeds
1.1 name of planting variety: YC-1804
1.2 test methods: the carbon-based complex enzyme fertilizer obtained in example 1 was diluted 50 times and 100 times, and then mixed with seeds, and germinated after drying in the shade, and the control group 1 was set to mix with seeds without using the carbon-based complex enzyme fertilizer.
The method specifically comprises the following steps:
(1) breaking the dormancy: placing the rice seeds in a 50 ℃ oven for 48 hours
(2) Seed soaking: taking out the seeds in the oven, and soaking the rice seeds after breaking dormancy in clear water for 48h
(3) And (3) disinfection: after seed soaking, the mixture is treated by the following steps of 1: soaking rice seeds for 1h by using ethylicin diluted by 5000 times for disinfection, washing the rice seeds for at least 3 times by using clear water after the disinfection is finished, and washing the ethylicin completely.
(4) Accelerating germination: immersing the rice seeds in water, placing the rice seeds in a thermostat at 32 ℃, standing for 12 hours, taking care not to evaporate the water to dryness, immersing the rice seeds in the water, and covering the seeds with a layer of gauze.
(5) Seed dressing: and (2) diluting the treated rice seeds by 50 times and 100 times respectively by using a carbon-based compound enzyme fertilizer, a carbon-based compound enzyme fertilizer and the like for seed dressing, wherein the seed dressing means that the rice seeds are slightly moistened in appearance by using the enzyme fertilizer, namely, the rice seeds are wrapped by a layer of coat, and the rice seeds are sowed in the shade after 24 hours.
(6) Sowing: the rice seeds which are not mixed with the seeds and are diluted by 50 times and mixed with 100 times are placed in glass culture dishes for germination, 50 seeds are placed in each culture dish, and the control group and the test group are respectively repeated for 3 times.
(7) And (4) determining the result: the culture dish was placed in a climatic chamber under light for growth, water was changed at 9 am and 5 am, and after half a month, the length of the bud, the length of the root and the germination rate were measured, and the results are shown in table 1.
TABLE 1 Rice index determination results table
| Germination rate | Average root length (mm) | Average bud Length (mm) | |
| Control group 1 | 90％ | 51.14 | 48.92 |
| Dressing seeds with stock solution | 58％ | 3.86 | 16.04 |
| Diluting by 50 times | 94％ | 71.29 | 59.11 |
| Diluting by 100 times | 92％ | 55.69 | 40.92 |
1.3 conclusion of the test: compared with the control group 1, the germination rate of the rice is improved by 4 percent, the root length is improved by 40 percent, and the bud length is improved by 23 percent by using the carbon-based compound enzyme fertilizer diluted by 50 times for seed dressing. The germination rate of rice is improved by 2% and the root length is improved by 8% by using a carbon-based compound enzyme fertilizer diluted by 100 times for seed dressing. The seed dressing with the stock solution can cause seedling burning, slow growth of rice and low germination rate. The carbon-based compound enzyme fertilizer is diluted by 50 times and 100 times, and is beneficial to the improvement of the germination rate of rice and the growth of rice in the bud stage.
2. The influence of the carbon-based compound enzyme fertilizer on the yield of the wheat after seed dressing
2.1 name of planting variety: albo
2.2 test methods: the test was carried out using the carbon-based complex enzyme fertilizer obtained in example 3, and the control group 2 was set to carry out seed dressing and spraying without using the carbon-based complex enzyme fertilizer. The specific test methods are shown in Table 2.
TABLE 2
2.3 comparison of the time differences of emergence of wheat after sowing of wheat is shown in Table 3.
TABLE 3
2.4 post harvest yields are shown in Table 4.
TABLE 4
2.5 conclusion of the test: the carbon-based compound enzyme fertilizer disclosed by the invention is used for dressing seeds with wheat, so that the growth of the wheat is promoted, and the yield is obviously increased. 479 kg of yield per mu is treated, and 10kg of yield is increased compared with the yield of the control group 2; 489.5 kg of yield per mu is treated, and the yield is increased by 20.5 kg compared with the control group 2; the yield of treated 3 mu is 513.5 kg, which is 44.5 kg higher than that of the control group 2; therefore, the carbon-based compound enzyme fertilizer has great effect of promoting the growth of wheat and obvious effect of increasing the yield by seed dressing, spraying and flushing.
The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. The carbon-based compound enzyme fertilizer is characterized by comprising the following raw materials in parts by weight: 1-80 parts of agricultural, forestry and livestock waste fermentation liquor or extract, 0.1-10 parts of humic acid, 1-20 parts of amylase, 2-35 parts of invertase, 1-15 parts of protease, 3-30 parts of bacillus subtilis, 4-35 parts of yeast, 0.1-10 parts of fertilizer containing carbon, nitrogen, phosphorus, potassium, sulfur, calcium and magnesium elements, 0.1-5 parts of fertilizer containing iron, manganese, zinc, boron and rare earth elements and 10-35 parts of multi-source plant complex enzyme.
2. The carbon-based compound enzyme fertilizer as claimed in claim 1, wherein the amylase is alpha-amylase or beta-amylase.
3. The carbon-based compound enzyme fertilizer as claimed in claim 1, wherein the invertase is vacuolar sucrase and/or cytoplasmic sucrase.
4. The carbon-based compound enzyme fertilizer as claimed in claim 1, wherein the protease is one or a mixture of serine protease, thiol protease or metallo protease.
5. The carbon-based compound enzyme fertilizer as claimed in claim 1, wherein the fertilizer containing the elements of nitrogen, phosphorus, potassium, sulfur, calcium and magnesium is a mixture of calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and magnesium sulfate.
6. The carbon-based compound enzyme fertilizer as claimed in claim 1, wherein the fertilizer containing the iron, manganese, zinc and boron rare earth elements is a mixture of ferrous sulfate, manganese sulfate, zinc sulfate, boric acid and mixed rare earth nitrate.
7. A preparation method of a carbon-based compound enzyme fertilizer is characterized by comprising the following steps:
(1) weighing the raw materials of the carbon-based compound enzyme fertilizer according to any one of claims 1-6;
(2) mixing and heating agricultural and forestry animal waste fermentation liquor or extract, humic acid, amylase, invertase, protease, bacillus subtilis, saccharomycetes, a fertilizer containing carbon, nitrogen, phosphorus, potassium, sulfur, calcium and magnesium elements, a fertilizer containing iron, manganese, zinc, boron and rare earth elements and a multi-source plant complex enzyme to 30 ℃, fermenting for 10-15 days at constant temperature, and cooling to room temperature to obtain the carbon-based complex enzyme fertilizer.
8. The preparation method of the carbon-based complex enzyme fertilizer as claimed in claim 7, wherein the preparation method of the agricultural and forestry animal waste fermentation liquid or the extracting solution comprises the following steps: crushing the agricultural and forestry waste, mixing the crushed agricultural and forestry waste with livestock excrement, adding a leavening agent, stirring, pouring the mixture into a fermentation tank, covering the fermentation tank with a plastic film, fermenting at normal temperature, and performing dry-wet separation after three months to obtain a liquid part, namely agricultural and forestry and livestock waste fermentation liquid;
or crushing the agricultural and forestry waste and then carrying out dry distillation to obtain the wood vinegar, namely the agricultural and forestry and animal waste extracting solution.
9. The preparation method of the carbon-based compound enzyme fertilizer as claimed in claim 7, wherein the preparation method of the multi-source plant compound enzyme comprises the following steps: crushing rice, fructus Lycii, fructus Mali Pumilae, fructus Actinidiae chinensis, and semen Maydis, and extracting at room temperature by dilute salt method.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115557814A (en) * | 2022-01-12 | 2023-01-03 | 山东坤容生态工程有限公司 | Compound enzyme water-soluble fertilizer and application thereof |
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