CN113229126A - Wild planting method for dendrobium huoshanense - Google Patents

Wild planting method for dendrobium huoshanense Download PDF

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Publication number
CN113229126A
CN113229126A CN202110456932.7A CN202110456932A CN113229126A CN 113229126 A CN113229126 A CN 113229126A CN 202110456932 A CN202110456932 A CN 202110456932A CN 113229126 A CN113229126 A CN 113229126A
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tissue culture
planting
linen
seedlings
wild
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CN202110456932.7A
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Chinese (zh)
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陈大卫
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00Fertilisers from household or town refuse
    • C05F9/04Biological compost
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

Abstract

The invention discloses a wild planting method of dendrobium huoshanense, which comprises the following steps of S1 selecting and processing tissue culture seedlings, selecting high-quality tissue culture seedlings which are free of diseases, thick in rootstock and mechanical damage in a tissue culture room, and continuously placing the selected tissue culture seedlings in a ventilated and normal room temperature culture room for 7-8 days. By adopting the wild planting method of dendrobium huoshanense provided by the invention, the culture medium is placed in the linen, and then the linen is bound on the trunk of the pine tree, so that the root of the tissue culture seedling can be effectively prevented from being influenced by pine oil, meanwhile, pests on the pine tree can not bite the root of the tissue culture seedling, and sprayed water or nutrient solution can be blocked by the linen and is retained in the culture medium, the problem that moisture and nutrient solution easily permeate into soil in the traditional foundation planting is effectively solved, the protection performance on the dendrobium huoshanense tissue culture seedling is stronger, the survival rate of the tissue culture seedling is improved, and the attractiveness is higher.

Description

Wild planting method for dendrobium huoshanense
Technical Field
The invention relates to the technical field of dendrobium nobile cultivation, in particular to a wild dendrobium huoshanense cultivation method.
Background
Dendrobium huoshanense is a geographical sign product of China, is mainly produced in Huoshan county of Anhui province in Dabie mountain areas, has the effects of improving eyesight, regulating yin and yang, tonifying yang and kidney, and beautifying, and is called the first of Jiuda xiancao.
Because dendrobium huoshanense mostly grows between cliff stone gaps and on ancient saplings in cloud and mist-lying cliff, and meanwhile, with the rapid development of modernization, wild dendrobium is nearly extinct, the rapid development of artificial planting industry is brought forward, although the development of dendrobium planting industry is rapid, the technology of imitating wild planting of dendrobium is not perfect, most of the existing dendrobium imitating wild planting is to paint a dendrobium culture medium on the surface of a trunk of a pine or a yellow cypress tree, and a dendrobium tissue culture seedling is planted on a culture medium, the root of the dendrobium tissue culture seedling is easy to expose outside by the culture method, the dendrobium tissue culture seedling is very easy to be affected by pine oil or pests, the protection performance on the root of the dendrobium tissue culture seedling is poor, and the survival rate and the yield of the tissue culture seedling are greatly reduced.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a wild dendrobium huoshanense planting method.
In order to achieve the purpose, the invention adopts the following technical scheme:
a wild planting method of dendrobium huoshanense comprises the following steps,
s1 selecting and processing tissue culture seedlings:
selecting high-quality tissue culture seedlings without diseases, thick rootstocks and mechanical damage in a tissue culture room, and continuously placing the selected tissue culture seedlings in a ventilated and normal-room-temperature culture room for 7-8 days;
s2 cultivation of tissue culture seedlings:
selecting pine barks from a field forest as a cultivation base layer, putting the pine barks on a hardening bed of a planting shed, planting tissue culture seedlings on the fermented pine barks, wherein the hardening time is six months to one year;
s3 selecting a wild planting field:
selecting a forest land with pine dense forest, clean water source and no industrial pollution as a planting field, and cleaning up the miscellaneous branches on the trunk surface of the pine;
preparation of S4 culture:
uniformly stirring the fermented pig manure, chicken manure and sheep manure mixture, the wood chip and wood residue mixture and the volcanic ash in a stirrer to form a culture medium for later use;
s5 wild fixed planting of tissue culture seedlings:
putting the culture medium obtained in the step S4 into white or light-beige linen, tightly wrapping the linen, annularly wrapping the linen with the culture medium on the treated pine tree trunk, planting the tissue culture seedling in the culture medium by penetrating the linen, smearing a layer of bryophyte at the position where the tissue culture seedling is contacted with the linen, binding and fixing the bryophyte, and fixing the tissue culture seedling on the linen by using a line card.
Preferably, the mixture of pig manure, chicken manure and sheep manure fermented in the step S4 accounts for 25%, the mixture of wood chips and wood residues accounts for 65%, and the volcanic ash accounts for 10%.
Preferably, the planting number of the tissue culture seedlings on the surface of the linen is 5 multiplied by 5 clusters per square meter, and each cluster is 3-5 tissue culture seedlings.
Preferably, after the tissue culture seedling is subjected to the fixed cultivation in the step S5, a fertilizer is used for regularly spraying a nutrient solution to the tissue culture seedling, and the nutrient solution is obtained by fermenting biogas slurry raw juice, equal parts of sheep manure, pig manure and vegetable seeds, then adding clear water and filtering.
Preferably, after the tissue culture seedlings are subjected to fixed planting, a layer of straw is wrapped around the linen in winter for antifreezing treatment.
The invention has the following beneficial effects:
1. by adopting the wild planting method of dendrobium huoshanense provided by the invention, the culture medium is placed in the linen, and then the linen is bound on the trunk of the pine tree, so that the root of the tissue culture seedling can be effectively prevented from being influenced by pine oil, meanwhile, pests on the pine tree can not bite the root of the tissue culture seedling, and sprayed water or nutrient solution can be blocked by the linen and is retained in the culture medium, the problem that moisture and nutrient solution easily permeate into soil in the traditional foundation planting is effectively solved, the protection performance on the dendrobium huoshanense tissue culture seedling is stronger, the survival rate of the tissue culture seedling is improved, and the attractiveness is higher.
2. By adopting the wild planting method of dendrobium huoshanense provided by the invention, as the biogas slurry raw juice is added in the nutrient solution, the nutrient solution is rich in selenium element, and the dendrobium huoshanense tissue culture seedlings are irrigated regularly through the selenium-rich nutrient solution, the dendrobium can contain a certain selenium element in the dendrobium after being picked, the functions of aging prevention, cancer resistance and the like of a human body can be improved, the functionality of the dendrobium huoshanense is improved, and the health care effect on the human body is better.
Detailed Description
Example 1
A wild planting method of dendrobium huoshanense comprises the following steps,
s1 selecting and processing tissue culture seedlings:
selecting high-quality tissue culture seedlings without diseases, thick rootstocks and mechanical damage in a tissue culture room, and continuously placing the selected tissue culture seedlings in a ventilated and normal room temperature culture room for 7 days;
s2 cultivation of tissue culture seedlings:
selecting pine barks from a field forest as a cultivation base layer, putting the pine barks on a hardening bed of a planting shed, planting tissue culture seedlings on the fermented pine barks, wherein the hardening time is one year;
s3 selecting a wild planting field:
selecting a forest land with pine dense forest, clean water source and no industrial pollution as a planting field, and cleaning up the miscellaneous branches on the trunk surface of the pine;
preparation of S4 culture:
uniformly stirring the fermented pig manure, chicken manure and sheep manure mixture, the wood chip and wood residue mixture and the volcanic ash in a stirrer to form a culture medium for later use;
s5 wild fixed planting of tissue culture seedlings:
putting the culture medium obtained in the step S4 into white or light-beige linen, tightly wrapping the linen, annularly wrapping the linen with the culture medium on the treated pine tree trunk, planting the tissue culture seedling in the culture medium by penetrating the linen, smearing a layer of bryophyte at the position where the tissue culture seedling is contacted with the linen, binding and fixing the bryophyte, and fixing the tissue culture seedling on the linen by utilizing a line card to realize the wild fixed planting of the tissue culture seedling.
The mixture of the pig manure, the chicken manure and the sheep manure fermented in the step S4 accounts for 25%, the mixture of the wood chips and the wood residues accounts for 65%, and the volcanic ash accounts for 10%.
The planting number of the tissue culture seedlings on the surface of the linen is 5 multiplied by 5 clusters per square meter, and each cluster is 3 tissue culture seedlings.
After the tissue culture seedlings are subjected to the fixed cultivation in the step S5, fertilizer is used for spraying nutrient solution regularly to the tissue culture seedlings, and the nutrient solution is obtained by fermenting biogas slurry raw juice, equal parts of sheep manure, pig manure and vegetable seeds, then adding clear water and filtering.
After the tissue culture seedlings are subjected to fixed planting, a layer of straw is wrapped around the linen in winter, and anti-freezing treatment is carried out.
Example 2
Unlike example 1, the method comprises the following steps,
s1 selecting and processing tissue culture seedlings:
selecting high-quality tissue culture seedlings without diseases, thick rootstocks and mechanical damage in a tissue culture room, and continuously placing the selected tissue culture seedlings in a ventilated and normal room temperature culture room for 7 days;
s2 cultivation of tissue culture seedlings:
selecting pine barks from a field forest as a cultivation base layer, putting the pine barks on a hardening bed of a planting shed, planting tissue culture seedlings on the fermented pine barks, wherein the hardening time is one year;
s3 selecting a wild planting field:
selecting a forest land with pine dense forest, clean water source and no industrial pollution as a planting field, and cleaning up the miscellaneous branches on the trunk surface of the pine;
preparation of S4 culture:
uniformly stirring the fermented pig manure, chicken manure and sheep manure mixture, the wood chip and wood residue mixture and the volcanic ash in a stirrer to form a culture medium for later use;
s5 wild fixed planting of tissue culture seedlings:
and (4) coating the culture medium obtained in the step S4 on the trunk surface of the pine tree, directly planting the tissue culture seedlings in the culture medium, fixing the tissue culture seedlings on the culture medium by using a line card, and fixedly binding the whole culture medium on the trunk surface of the pine tree.
The mixture of the pig manure, the chicken manure and the sheep manure fermented in the step S4 accounts for 25%, the mixture of the wood chips and the wood residues accounts for 65%, and the volcanic ash accounts for 10%.
The planting number of the tissue culture seedlings on the surface of the linen is 5 multiplied by 5 clusters per square meter, and each cluster is 3 tissue culture seedlings.
After the tissue culture seedlings are subjected to fixed planting, a layer of straw is wrapped around the linen in winter, and anti-freezing treatment is carried out.
In order to compare the effects of the invention, the same batch of cultured tissue culture seedlings are selected as experimental objects, the tissue culture seedlings in the embodiment 1 and the embodiment 2 are respectively cultured in different fixed planting modes, the yield and the selenium content ratio of the cultured dendrobium huoshanense are measured, and the specific comparison data are shown in table 1:
TABLE 1
Yield (Kg) Selenium content (Mg)
Example 1 256 0.025
Example 2 202 0.012
As can be seen from table 1, embodiment 1 adopts the mode of linen parcel culture medium to come to surely plant the group banks up seedling, can effectively prevent pine oil and pest to the influence of group banks up seedling, greatly increased the output of dendrobium huoshanense, adopts rich selenium nutrient solution to carry out periodic irrigation simultaneously, can increase the selenium content of dendrobium huoshanense, and is better to the health care effect of human body, has obviously promoted the benefit, has increased market competition.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (5)

1. A wild planting method of dendrobium huoshanense comprises the following steps:
s1 selecting and processing tissue culture seedlings:
selecting high-quality tissue culture seedlings without diseases, thick rootstocks and mechanical damage in a tissue culture room, and continuously placing the selected tissue culture seedlings in a ventilated and normal-room-temperature culture room for 7-8 days;
s2 cultivation of tissue culture seedlings:
selecting pine barks from a field forest as a cultivation base layer, putting the pine barks on a hardening bed of a planting shed, planting tissue culture seedlings on the fermented pine barks, wherein the hardening time is six months to one year;
s3 selecting a wild planting field:
selecting a forest land with pine dense forest, clean water source and no industrial pollution as a planting field, and cleaning up the miscellaneous branches on the trunk surface of the pine;
preparation of S4 culture:
uniformly stirring the fermented pig manure, chicken manure and sheep manure mixture, the wood chip and wood residue mixture and the volcanic ash in a stirrer to form a culture medium for later use;
s5 wild fixed planting of tissue culture seedlings:
putting the culture medium obtained in the step S4 into white or light-beige linen, tightly wrapping the linen, annularly wrapping the linen with the culture medium on the treated pine tree trunk, planting the tissue culture seedling in the culture medium by penetrating the linen, smearing a layer of bryophyte at the position where the tissue culture seedling is contacted with the linen, binding and fixing the bryophyte, and fixing the tissue culture seedling on the linen by using a line card.
2. The method for wild planting of dendrobium huoshanense as claimed in claim 1, wherein the method comprises the following steps: the ratio of the mixture of the pig manure, the chicken manure and the sheep manure fermented in the step S4 is 25%, the ratio of the mixture of the wood chips and the wood residues is 65%, and the ratio of the volcanic ash is 10%.
3. The method for wild planting of dendrobium huoshanense as claimed in claim 1, wherein the method comprises the following steps: the planting number of the tissue culture seedlings on the surface of the linen is 5 multiplied by 5 clusters per square meter, and each cluster is 3-5 tissue culture seedlings.
4. The method for wild planting of dendrobium huoshanense as claimed in claim 1, wherein the method comprises the following steps: and after the tissue culture seedlings are subjected to the fixed cultivation in the step S5, regularly spraying nutrient solution by using a fertilizer, wherein the nutrient solution is obtained by fermenting biogas slurry raw juice, equal parts of sheep manure, pig manure and vegetable seeds, then adding clear water and filtering.
5. The method for wild planting of dendrobium huoshanense as claimed in claim 1, wherein the method comprises the following steps: and after the fixed planting of the tissue culture seedlings is finished, wrapping a layer of straw around the linen in winter, and performing anti-freezing treatment.
CN202110456932.7A 2021-04-27 2021-04-27 Wild planting method for dendrobium huoshanense Pending CN113229126A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104737771A (en) * 2015-04-23 2015-07-01 孟连大发铁皮石斛有限公司 High-sugar dendrobium officinale planting method
CN105724212A (en) * 2016-02-25 2016-07-06 云南斛哥庄园有限公司 Imitation wild planting method of organic dendrobium nobile lindl
CN107343445A (en) * 2017-08-17 2017-11-14 邓万超 Pseudo-wild cultivating method under dendrobium candidum pine forests
CN107771659A (en) * 2016-08-27 2018-03-09 杜名胜 Huoshan dendrobium candidum bionic cultivation technology
CN108419659A (en) * 2018-04-19 2018-08-21 霍山县英豪石斛农民专业合作社 A kind of wild planting process of Dendrobidium huoshanness

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104737771A (en) * 2015-04-23 2015-07-01 孟连大发铁皮石斛有限公司 High-sugar dendrobium officinale planting method
CN105724212A (en) * 2016-02-25 2016-07-06 云南斛哥庄园有限公司 Imitation wild planting method of organic dendrobium nobile lindl
CN107771659A (en) * 2016-08-27 2018-03-09 杜名胜 Huoshan dendrobium candidum bionic cultivation technology
CN107343445A (en) * 2017-08-17 2017-11-14 邓万超 Pseudo-wild cultivating method under dendrobium candidum pine forests
CN108419659A (en) * 2018-04-19 2018-08-21 霍山县英豪石斛农民专业合作社 A kind of wild planting process of Dendrobidium huoshanness

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Application publication date: 20210810