CN113227356A - 非贴附性细胞及其用途 - Google Patents

非贴附性细胞及其用途 Download PDF

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Publication number
CN113227356A
CN113227356A CN201980074754.2A CN201980074754A CN113227356A CN 113227356 A CN113227356 A CN 113227356A CN 201980074754 A CN201980074754 A CN 201980074754A CN 113227356 A CN113227356 A CN 113227356A
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cells
adherent
connective tissue
enriched population
cell
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Chinese (zh)
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亚寇夫·纳米雅思
梅拉夫·科恩
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Yissum Research Development Co of Hebrew University of Jerusalem
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Yissum Research Development Co of Hebrew University of Jerusalem
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980074754.2A 2018-11-08 2019-11-07 非贴附性细胞及其用途 Pending CN113227356A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862757275P 2018-11-08 2018-11-08
US62/757,275 2018-11-08
PCT/IL2019/051219 WO2020095305A1 (en) 2018-11-08 2019-11-07 Anchorage-independent cells and use thereof

Publications (1)

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CN113227356A true CN113227356A (zh) 2021-08-06

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US (1) US20210395690A1 (https=)
EP (1) EP3877508A1 (https=)
JP (2) JP7291217B2 (https=)
KR (4) KR102871263B1 (https=)
CN (1) CN113227356A (https=)
AU (1) AU2019377278C9 (https=)
IL (3) IL298567B2 (https=)
WO (1) WO2020095305A1 (https=)

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US20220195392A1 (en) * 2019-04-23 2022-06-23 The Regents Of The University Of California Methods and compositions for cell culture on heterogeneous scaffolds
WO2021148960A1 (en) * 2020-01-21 2021-07-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Utilization of fgf activators in culture media
JP2023539149A (ja) * 2020-08-21 2023-09-13 メルク パテント ゲーエムベーハー 食用中空繊維において増殖した筋肉細胞を用いて生成された可食組織様構造
JP2022072917A (ja) * 2020-10-30 2022-05-17 株式会社ダイセル 培養食肉複合体、及びその製造方法
AU2022222783A1 (en) * 2021-02-22 2023-09-07 Vitrolabs Inc Immortalized cell line for engineered synthetic leather
US20240191198A1 (en) * 2021-04-13 2024-06-13 Bluenalu, Inc. Cell-cultured food products and related cells, compositions, methods and systems
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
WO2024048588A1 (ja) * 2022-08-31 2024-03-07 インテグリカルチャー株式会社 細胞培養システム、培養肉、及び培養上清

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US20040005319A1 (en) * 1991-08-30 2004-01-08 The University Of South Florida Connective tissue growth factor
US20060148074A1 (en) * 1996-08-30 2006-07-06 Invitrogen Corporation Serum-free mammalian cell culture medium, and uses thereof
CN101688177A (zh) * 2007-02-12 2010-03-31 人类起源公司 来自贴壁胎盘干细胞的肝细胞和软骨细胞；以及cd34+、cd45-胎盘干细胞富集的细胞群
US20100190252A1 (en) * 2008-12-22 2010-07-29 Sphero Tec Gmbh Methods for the preparation of fibroblasts
CN103865875A (zh) * 2012-12-18 2014-06-18 中国科学院遗传与发育生物学研究所 一种用成纤维细胞制备神经干细胞的方法
CN104830752A (zh) * 2015-03-05 2015-08-12 中国农业大学 一种猪胎儿成纤维细胞的单细胞培养方法
CN107460156A (zh) * 2016-06-03 2017-12-12 北京大北农科技集团股份有限公司动物医学研究中心 无血清全悬浮mdck细胞株及其在生产流感病毒中的应用
WO2018011805A2 (en) * 2016-07-11 2018-01-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Systems and methods for growing cells in vitro
CN107841481A (zh) * 2016-09-19 2018-03-27 深圳华大方舟生物技术有限公司 一种用于单细胞培养的培养基及其制备方法和应用
CN116121174A (zh) * 2022-09-30 2023-05-16 山东农业大学 一种鸡胚胎成纤维细胞体外三维培养和分离的方法

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US20100190252A1 (en) * 2008-12-22 2010-07-29 Sphero Tec Gmbh Methods for the preparation of fibroblasts
CN103865875A (zh) * 2012-12-18 2014-06-18 中国科学院遗传与发育生物学研究所 一种用成纤维细胞制备神经干细胞的方法
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CN107460156A (zh) * 2016-06-03 2017-12-12 北京大北农科技集团股份有限公司动物医学研究中心 无血清全悬浮mdck细胞株及其在生产流感病毒中的应用
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CN116121174A (zh) * 2022-09-30 2023-05-16 山东农业大学 一种鸡胚胎成纤维细胞体外三维培养和分离的方法

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KR20240004541A (ko) 2024-01-11
KR20230091997A (ko) 2023-06-23
AU2019377278C9 (en) 2025-08-14
IL298567A (en) 2023-01-01
IL282976B1 (en) 2023-01-01
EP3877508A1 (en) 2021-09-15
JP2022509054A (ja) 2022-01-20
IL298567B1 (en) 2023-06-01
KR102605256B1 (ko) 2023-11-24
KR20210091226A (ko) 2021-07-21
AU2019377278A1 (en) 2021-06-03
KR102871263B1 (ko) 2025-10-16
IL298567B2 (en) 2023-10-01
WO2020095305A1 (en) 2020-05-14
JP7291217B2 (ja) 2023-06-14
IL302532A (en) 2023-07-01
AU2019377278C1 (en) 2025-07-24
IL282976B2 (en) 2023-05-01
IL282976A (en) 2021-06-30
JP2023055867A (ja) 2023-04-18
KR20250162599A (ko) 2025-11-18
US20210395690A1 (en) 2021-12-23
BR112021008808A2 (pt) 2021-09-08
AU2019377278B2 (en) 2022-11-17

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