AU2019377278C9 - Anchorage-independent cells and use thereof - Google Patents

Anchorage-independent cells and use thereof

Info

Publication number
AU2019377278C9
AU2019377278C9 AU2019377278A AU2019377278A AU2019377278C9 AU 2019377278 C9 AU2019377278 C9 AU 2019377278C9 AU 2019377278 A AU2019377278 A AU 2019377278A AU 2019377278 A AU2019377278 A AU 2019377278A AU 2019377278 C9 AU2019377278 C9 AU 2019377278C9
Authority
AU
Australia
Prior art keywords
cells
fibroblasts
composition
thecomposition
anchorage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2019377278A
Other languages
English (en)
Other versions
AU2019377278A1 (en
AU2019377278C1 (en
AU2019377278B2 (en
Inventor
Merav Cohen
Yaakov Nahmias
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=68808476&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2019377278(C9) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of AU2019377278A1 publication Critical patent/AU2019377278A1/en
Application granted granted Critical
Publication of AU2019377278B2 publication Critical patent/AU2019377278B2/en
Publication of AU2019377278C1 publication Critical patent/AU2019377278C1/en
Publication of AU2019377278C9 publication Critical patent/AU2019377278C9/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2019377278A 2018-11-08 2019-11-07 Anchorage-independent cells and use thereof Active AU2019377278C9 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862757275P 2018-11-08 2018-11-08
US62/757,275 2018-11-08
PCT/IL2019/051219 WO2020095305A1 (en) 2018-11-08 2019-11-07 Anchorage-independent cells and use thereof

Publications (4)

Publication Number Publication Date
AU2019377278A1 AU2019377278A1 (en) 2021-06-03
AU2019377278B2 AU2019377278B2 (en) 2022-11-17
AU2019377278C1 AU2019377278C1 (en) 2025-07-24
AU2019377278C9 true AU2019377278C9 (en) 2025-08-14

Family

ID=68808476

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019377278A Active AU2019377278C9 (en) 2018-11-08 2019-11-07 Anchorage-independent cells and use thereof

Country Status (8)

Country Link
US (1) US20210395690A1 (https=)
EP (1) EP3877508A1 (https=)
JP (2) JP7291217B2 (https=)
KR (4) KR102871263B1 (https=)
CN (1) CN113227356A (https=)
AU (1) AU2019377278C9 (https=)
IL (3) IL298567B2 (https=)
WO (1) WO2020095305A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220195392A1 (en) * 2019-04-23 2022-06-23 The Regents Of The University Of California Methods and compositions for cell culture on heterogeneous scaffolds
WO2021148960A1 (en) * 2020-01-21 2021-07-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Utilization of fgf activators in culture media
JP2023539149A (ja) * 2020-08-21 2023-09-13 メルク パテント ゲーエムベーハー 食用中空繊維において増殖した筋肉細胞を用いて生成された可食組織様構造
JP2022072917A (ja) * 2020-10-30 2022-05-17 株式会社ダイセル 培養食肉複合体、及びその製造方法
AU2022222783A1 (en) * 2021-02-22 2023-09-07 Vitrolabs Inc Immortalized cell line for engineered synthetic leather
US20240191198A1 (en) * 2021-04-13 2024-06-13 Bluenalu, Inc. Cell-cultured food products and related cells, compositions, methods and systems
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
WO2024048588A1 (ja) * 2022-08-31 2024-03-07 インテグリカルチャー株式会社 細胞培養システム、培養肉、及び培養上清

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US5019369A (en) 1984-10-22 1991-05-28 Vestar, Inc. Method of targeting tumors in humans
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5770209A (en) * 1991-08-30 1998-06-23 University Of South Florida Acceleration of wound healing using connective tissue growth factor
US7384634B2 (en) * 1991-08-30 2008-06-10 University Of South Florida Connective tissue growth factor
AU4330597A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
US8026096B1 (en) * 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
JP3402304B2 (ja) 2000-03-30 2003-05-06 株式会社微生物化学研究所 猫クラミジアワクチンの製造方法
JP2006246715A (ja) 2005-03-08 2006-09-21 Osaka Bioscience Institute 足場非依存的増殖能を指標とした癌遺伝子及びその機能阻害物質の探索方法
AU2008216748A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and CD34+, CD45- placental stem cell-enriched cell populations
US20110236356A1 (en) * 2008-12-12 2011-09-29 Nova Southeastern University Methods of isolating and using stem cells
EP2199383B1 (en) * 2008-12-22 2017-02-15 SpheroTec GmbH Methods for the preparation of fibroblasts
US20160097109A1 (en) * 2012-03-28 2016-04-07 Gabor Forgacs Engineered leather and methods of manufacture thereof
CN103865875B (zh) * 2012-12-18 2016-08-10 中国科学院遗传与发育生物学研究所 一种用成纤维细胞制备神经干细胞的方法
JP6986968B2 (ja) 2014-12-04 2021-12-22 ステモン・インコーポレイテッド エネルギーを利用した多能性細胞誘導装置及び方法
CN104830752B (zh) * 2015-03-05 2018-05-22 中国农业大学 一种猪胎儿成纤维细胞的单细胞培养方法
CN107460156B (zh) * 2016-06-03 2022-03-11 兆丰华生物科技(南京)有限公司北京生物医药科技中心 无血清全悬浮mdck细胞株及其在生产流感病毒中的应用
EP3481191A2 (en) 2016-07-11 2019-05-15 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Systems and methods for growing cells in vitro
CN107841481A (zh) * 2016-09-19 2018-03-27 深圳华大方舟生物技术有限公司 一种用于单细胞培养的培养基及其制备方法和应用
GB201619876D0 (en) * 2016-11-24 2017-01-11 Cambridge Entpr Ltd Controllable transcription
CA3066060A1 (en) * 2017-06-07 2018-12-13 Wild Type, Inc. Ex vivo meat production
US10669524B2 (en) * 2018-06-12 2020-06-02 Fork & Goode, Inc. Large scale cell culture system for making meat and associated products
CN116121174B (zh) * 2022-09-30 2026-04-03 山东农业大学 一种鸡胚胎成纤维细胞体外三维培养和分离的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Kemp, R., 'Cytotoxicity in an anchorage-independent fibroblast cell line measured by a combination of fluorescent dyes', 1995, In Vitro Toxicity Testing Protocols, Humana Press, pages 211-218. *

Also Published As

Publication number Publication date
KR20240004541A (ko) 2024-01-11
KR20230091997A (ko) 2023-06-23
IL298567A (en) 2023-01-01
IL282976B1 (en) 2023-01-01
EP3877508A1 (en) 2021-09-15
JP2022509054A (ja) 2022-01-20
IL298567B1 (en) 2023-06-01
KR102605256B1 (ko) 2023-11-24
KR20210091226A (ko) 2021-07-21
AU2019377278A1 (en) 2021-06-03
KR102871263B1 (ko) 2025-10-16
IL298567B2 (en) 2023-10-01
WO2020095305A1 (en) 2020-05-14
JP7291217B2 (ja) 2023-06-14
IL302532A (en) 2023-07-01
AU2019377278C1 (en) 2025-07-24
IL282976B2 (en) 2023-05-01
IL282976A (en) 2021-06-30
JP2023055867A (ja) 2023-04-18
KR20250162599A (ko) 2025-11-18
CN113227356A (zh) 2021-08-06
US20210395690A1 (en) 2021-12-23
BR112021008808A2 (pt) 2021-09-08
AU2019377278B2 (en) 2022-11-17

Similar Documents

Publication Publication Date Title
AU2019377278C9 (en) Anchorage-independent cells and use thereof
Li et al. A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells
US11339372B2 (en) Serum-free medium inducing differentiation of umbilical cord mesenchymal stem cell into insulin-secretion-like cell and preparation method and use thereof
KR102680315B1 (ko) 식물성 단백질을 포함하는 다공성 세포 지지체 및 이를 이용하여 제조된 배양육
WO2016127908A1 (zh) 一种三维复合细胞聚集体模型及其制备方法与应用
CN105267243B (zh) 一种消除皮肤妊娠纹的干细胞提取物
JP2022509054A5 (https=)
CN112574946A (zh) 一种原代分离培养土狗多个组织来源的成纤维细胞及其永生化的构建方法
Chen et al. Efficient manufacturing of tissue engineered cartilage in vitro by a multiplexed 3D cultured method
CN113717933A (zh) Fgf7在制备干细胞扩增与表型维持试剂中的应用
Muhitch et al. Characterization of aggregation and protein expression of bovine corneal endothelial cells as microcarrier cultures in a rotating-wall vessel
BR112021008808B1 (pt) Composição compreendendo uma população enriquecida de células e métodos para produzir uma linha celular independente de ancoragem e para diminuir o tempo de duplicação destas
Qi et al. Comparative study on seeding methods of human bone marrow stromal cells in bone tissue engineering
Mahboudi et al. Comparison between high cell-density culture systems for chondrogenic differentiation and articular cartilage reconstruction of human mesenchymal stem cells: a literature review
CN113667632A (zh) 细胞培养基添加剂、细胞培养基及细胞体外扩增的方法
US20260107959A1 (en) Hybrid cultured meat products and related methods
US20230399605A1 (en) Porous scaffold, method of making and uses thereof
CN119060950A (zh) 细胞膜片、脱细胞基质材料及其制备方法
JP2025539461A (ja) 筋前駆細胞（ｍｐｃ）の大量培養物の調製方法およびその使用
CN113201496A (zh) 一种高对比明显度的干细胞体外稳定性的检测方法
WO2025262230A1 (en) In vitro method of producing milk
Su et al. Construction of a gelatin-based composite cultured meat model with tunable muscle-fat ratio mimicking beef characteristics

Legal Events

Date Code Title Description
CB Opposition filed

Opponent name: VOW GROUP PTY LTD

DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 16 NOV 2023

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT FILED 16 NOV 2023

DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 18 OCT 2024

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT FILED 18 OCT 2024

SREP Specification republished