KR102871263B1 - 부착 비의존성 세포 및 그 용도 - Google Patents

부착 비의존성 세포 및 그 용도

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Publication number
KR102871263B1
KR102871263B1 KR1020237039644A KR20237039644A KR102871263B1 KR 102871263 B1 KR102871263 B1 KR 102871263B1 KR 1020237039644 A KR1020237039644 A KR 1020237039644A KR 20237039644 A KR20237039644 A KR 20237039644A KR 102871263 B1 KR102871263 B1 KR 102871263B1
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cells
connective tissue
anchorage
cell
independent
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Korean (ko)
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KR20240004541A (ko
Inventor
야콥 나미아스
메라브 코헨
Original Assignee
이슘 리서치 디벨롭먼트 컴퍼니 오브 더 히브루 유니버시티 오브 예루살렘 엘티디.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=68808476&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR102871263(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 이슘 리서치 디벨롭먼트 컴퍼니 오브 더 히브루 유니버시티 오브 예루살렘 엘티디. filed Critical 이슘 리서치 디벨롭먼트 컴퍼니 오브 더 히브루 유니버시티 오브 예루살렘 엘티디.
Priority to KR1020257033681A priority Critical patent/KR20250162599A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020237039644A 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도 Active KR102871263B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020257033681A KR20250162599A (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862757275P 2018-11-08 2018-11-08
US62/757,275 2018-11-08
PCT/IL2019/051219 WO2020095305A1 (en) 2018-11-08 2019-11-07 Anchorage-independent cells and use thereof
KR1020237017466A KR102605256B1 (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020237017466A Division KR102605256B1 (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020257033681A Division KR20250162599A (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도

Publications (2)

Publication Number Publication Date
KR20240004541A KR20240004541A (ko) 2024-01-11
KR102871263B1 true KR102871263B1 (ko) 2025-10-16

Family

ID=68808476

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020237039644A Active KR102871263B1 (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도
KR1020237017466A Active KR102605256B1 (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도
KR1020217017600A Ceased KR20210091226A (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도
KR1020257033681A Pending KR20250162599A (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도

Family Applications After (3)

Application Number Title Priority Date Filing Date
KR1020237017466A Active KR102605256B1 (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도
KR1020217017600A Ceased KR20210091226A (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도
KR1020257033681A Pending KR20250162599A (ko) 2018-11-08 2019-11-07 부착 비의존성 세포 및 그 용도

Country Status (8)

Country Link
US (1) US20210395690A1 (https=)
EP (1) EP3877508A1 (https=)
JP (2) JP7291217B2 (https=)
KR (4) KR102871263B1 (https=)
CN (1) CN113227356A (https=)
AU (1) AU2019377278C9 (https=)
IL (3) IL298567B2 (https=)
WO (1) WO2020095305A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220195392A1 (en) * 2019-04-23 2022-06-23 The Regents Of The University Of California Methods and compositions for cell culture on heterogeneous scaffolds
WO2021148960A1 (en) * 2020-01-21 2021-07-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Utilization of fgf activators in culture media
JP2023539149A (ja) * 2020-08-21 2023-09-13 メルク パテント ゲーエムベーハー 食用中空繊維において増殖した筋肉細胞を用いて生成された可食組織様構造
JP2022072917A (ja) * 2020-10-30 2022-05-17 株式会社ダイセル 培養食肉複合体、及びその製造方法
AU2022222783A1 (en) * 2021-02-22 2023-09-07 Vitrolabs Inc Immortalized cell line for engineered synthetic leather
US20240191198A1 (en) * 2021-04-13 2024-06-13 Bluenalu, Inc. Cell-cultured food products and related cells, compositions, methods and systems
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
WO2024048588A1 (ja) * 2022-08-31 2024-03-07 インテグリカルチャー株式会社 細胞培養システム、培養肉、及び培養上清

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011805A2 (en) 2016-07-11 2018-01-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Systems and methods for growing cells in vitro

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US5019369A (en) 1984-10-22 1991-05-28 Vestar, Inc. Method of targeting tumors in humans
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5770209A (en) * 1991-08-30 1998-06-23 University Of South Florida Acceleration of wound healing using connective tissue growth factor
US7384634B2 (en) * 1991-08-30 2008-06-10 University Of South Florida Connective tissue growth factor
AU4330597A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
US8026096B1 (en) * 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
JP3402304B2 (ja) 2000-03-30 2003-05-06 株式会社微生物化学研究所 猫クラミジアワクチンの製造方法
JP2006246715A (ja) 2005-03-08 2006-09-21 Osaka Bioscience Institute 足場非依存的増殖能を指標とした癌遺伝子及びその機能阻害物質の探索方法
AU2008216748A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and CD34+, CD45- placental stem cell-enriched cell populations
US20110236356A1 (en) * 2008-12-12 2011-09-29 Nova Southeastern University Methods of isolating and using stem cells
EP2199383B1 (en) * 2008-12-22 2017-02-15 SpheroTec GmbH Methods for the preparation of fibroblasts
US20160097109A1 (en) * 2012-03-28 2016-04-07 Gabor Forgacs Engineered leather and methods of manufacture thereof
CN103865875B (zh) * 2012-12-18 2016-08-10 中国科学院遗传与发育生物学研究所 一种用成纤维细胞制备神经干细胞的方法
JP6986968B2 (ja) 2014-12-04 2021-12-22 ステモン・インコーポレイテッド エネルギーを利用した多能性細胞誘導装置及び方法
CN104830752B (zh) * 2015-03-05 2018-05-22 中国农业大学 一种猪胎儿成纤维细胞的单细胞培养方法
CN107460156B (zh) * 2016-06-03 2022-03-11 兆丰华生物科技(南京)有限公司北京生物医药科技中心 无血清全悬浮mdck细胞株及其在生产流感病毒中的应用
CN107841481A (zh) * 2016-09-19 2018-03-27 深圳华大方舟生物技术有限公司 一种用于单细胞培养的培养基及其制备方法和应用
GB201619876D0 (en) * 2016-11-24 2017-01-11 Cambridge Entpr Ltd Controllable transcription
CA3066060A1 (en) * 2017-06-07 2018-12-13 Wild Type, Inc. Ex vivo meat production
US10669524B2 (en) * 2018-06-12 2020-06-02 Fork & Goode, Inc. Large scale cell culture system for making meat and associated products
CN116121174B (zh) * 2022-09-30 2026-04-03 山东农业大学 一种鸡胚胎成纤维细胞体外三维培养和分离的方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011805A2 (en) 2016-07-11 2018-01-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Systems and methods for growing cells in vitro

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Pairault, J.& Green H. PNAS (1979) 76(10):5138-5142
Yan Zhang, Mei Yu, Xueyong Zhao, Minjia Dai, Chang Chen& Weidong Tian (2019) Optimizing adipose tissue extract isolation with stirred suspension culture, Connective Tissue Research, 60:2, 178-188

Also Published As

Publication number Publication date
KR20240004541A (ko) 2024-01-11
KR20230091997A (ko) 2023-06-23
AU2019377278C9 (en) 2025-08-14
IL298567A (en) 2023-01-01
IL282976B1 (en) 2023-01-01
EP3877508A1 (en) 2021-09-15
JP2022509054A (ja) 2022-01-20
IL298567B1 (en) 2023-06-01
KR102605256B1 (ko) 2023-11-24
KR20210091226A (ko) 2021-07-21
AU2019377278A1 (en) 2021-06-03
IL298567B2 (en) 2023-10-01
WO2020095305A1 (en) 2020-05-14
JP7291217B2 (ja) 2023-06-14
IL302532A (en) 2023-07-01
AU2019377278C1 (en) 2025-07-24
IL282976B2 (en) 2023-05-01
IL282976A (en) 2021-06-30
JP2023055867A (ja) 2023-04-18
KR20250162599A (ko) 2025-11-18
CN113227356A (zh) 2021-08-06
US20210395690A1 (en) 2021-12-23
BR112021008808A2 (pt) 2021-09-08
AU2019377278B2 (en) 2022-11-17

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