CN113197803B - Antibacterial mouth wash and preparation method thereof - Google Patents

Abstract

The invention provides an antibacterial mouthwash and a preparation method thereof, wherein the antibacterial mouthwash comprises the following components in percentage by weight: 5-10% of long-medicine eight-treasure extract, 0.1-0.5% of platycodon root juice, 3-5% of humectant, 0.01-0.1% of preservative, 0.1-0.5% of mineral salt, 2-20% of natural surfactant and the balance of deionized water. The invention innovatively provides the gargle which takes the eight-treasure extract of long-medicine as the main component for sterilizing and diminishing inflammation in the gargle, has the effects of sterilizing and diminishing inflammation, is safe and harmless, and can be used for a long time. Compared with a chemical surfactant, the natural surfactant is selected, so that the safety is higher, and the natural surfactant is more suitable for the consumption field.

Description

Antibacterial mouth wash and preparation method thereof

Technical Field

The invention relates to the technical field of medical oral care, relates to daily chemical products, and particularly relates to antibacterial mouthwash and a preparation method thereof.

Background

At present, the main components of the mouthwash are essence, surfactant, fluoride, strontium chloride, alcohol, water and the like, and the mouthwash can clean the oral cavity and prevent dental caries and periodontitis.

Generally, the bacteriostatic action of the existing mouthwash is mainly realized by a chemically synthesized bacteriostatic agent, for example, triclosan, chlorhexidine gluconate and the like are used as bacteriostatic agents, although the bacteriostatic effect is better, the safety of triclosan is a greater controversial in recent years; if the chlorhexidine gluconate is used for a long time, the dental stain is easily deposited on the surface of the tooth, which affects the taste and causes recurrent aphtha. In addition, more seriously, the mouth wash containing the chemical bacteriostatic agent is easy to cause the normal flora in the oral cavity to be disordered after being used for a long time, and is not suitable for being used for a long time.

Meanwhile, surfactants added into the existing mouthwash are mostly selected from monoglycerides, acetylated monoglycerides, sorbitan esters or sodium stearyl lactate, and the long-term use safety of the oral cavity is questioned.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide the antibacterial mouthwash and the preparation method thereof, and creatively provides the gargle which takes the eight-treasure extract of the long-acting medicine as the main component for sterilizing and diminishing inflammation in the mouthwash, has the effects of sterilizing and diminishing inflammation, is safe and harmless, and can be used for a long time. Compared with a chemical surfactant, the natural surfactant is selected, so that the safety is higher, and the natural surfactant is more suitable for the consumption field.

In order to achieve the purpose, the invention provides bacteriostatic mouth wash and a preparation method thereof, and the technical scheme is as follows:

the bacteriostatic mouth wash comprises the following components in percentage by weight: 5-10% of long-medicine eight-treasure extract, 0.1-0.5% of platycodon root juice, 3-5% of humectant, 0.01-0.1% of preservative, 0.1-0.5% of mineral salt, 2-20% of natural surfactant and the balance of deionized water.

Preferably, the composition comprises the following components in percentage by weight: 7-9% of long-medicine eight-treasure extract, 0.1-0.3% of platycodon root juice, 3-5% of humectant, 0.01-0.1% of preservative, 0.1-0.5% of mineral salt, 2-15% of natural surfactant and the balance of deionized water.

More preferably, the composition comprises the following components in percentage by weight: 8% of long-medicine eight-treasure extract, 0.2% of platycodon root juice, 3% of humectant, 0.05% of preservative, 0.2% of mineral salt, 11% of natural surfactant and the balance of deionized water.

Specifically, as another scheme, the bacteriostatic mouth wash of the invention can further comprise 0.1-0.3% of a solubilizer in percentage by weight.

Specifically, as another scheme, the bacteriostatic mouth wash of the invention may further comprise a flavoring agent 1.0-3.0% by weight.

Specifically, as another scheme, the bacteriostatic mouth wash can further comprise 0.02-0.1% of an organic silicon defoaming agent in percentage by weight.

In addition, the natural surfactant of the present invention includes an extract of emblic leafflower fruit and an extract of eucommia ulmoides leaves; wherein the source of the emblic extract comprises a mixture of fresh emblic kernels and emblic pulps.

The invention also discloses a preparation method of the antibacterial mouth wash, which comprises the following steps:

step S1, preparing a natural surfactant;

step 1: preparing an extract of folium cortex eucommiae;

step 2: preparing an emblic leafflower fruit extract: mixing fresh fructus Phyllanthi kernel and fructus Phyllanthi pulp, mincing, extracting with solvent for several times, and adding a certain amount of folium Eucommiae extract during the solvent extraction;

and step 3: mixing filtrates obtained after the solvent is extracted for multiple times, standing, filtering and concentrating to a small volume to obtain a natural surfactant;

step two, S2, preparing bacteriostatic mouth wash;

step S1, putting deionized water into a clean container, adding the preservative, the humectant and the mineral salt while stirring at room temperature until the preservative, the humectant and the mineral salt are completely dissolved and uniformly dispersed to prepare a phase A; step S2, adding the eight-treasure long-medicine extract, the platycodon root juice and the natural surfactant into the phase A, and stirring uniformly at room temperature until a stable clear solution system is formed; and step S3, standing at room temperature for a preset time, filtering, adding deionized water, and homogenizing to obtain the antibacterial mouthwash.

Importantly, the solvent in step one is extracted for multiple times, and comprises:

for the first time: performing shaking water extraction with hot water, adding folium Eucommiae extract during water extraction, standing, and filtering;

and (3) for the second time: extracting the first residue with mixture of water and alcohol, standing, and filtering.

More preferably, the solvent is extracted for a plurality of times, and specifically comprises:

for the first time: shaking with 80-90 deg.C hot water, extracting with folium Eucommiae extract, standing, and filtering;

and (3) for the second time: extracting the mixture of water and alcohol at 15 deg.C, standing, and filtering.

Preferably, the ratio of the material to the liquid in the first hot water shaking water extraction is 1: 12-15, time 4-6H; the mixing ratio of the second water and the alcohol is 2-4: 1, the ratio of material to liquid is 1: 10-12 hours and 2-4 hours.

Preferably, when the emblic leafflower fruit extract is extracted, the ratio of the fresh emblic leafflower fruit kernel to the emblic leafflower fruit pulp is 1: 2.5-3.

Specifically, the addition amount of the folium cortex eucommiae extract is 0.5-2% of the total weight of the feed liquid.

Preferably, the alcohol concentration is not less than 90 °.

The invention has the beneficial effects that:

(1) compared with the prior art, the antibacterial mouthwash provided by the invention innovatively provides the long-acting Chinese herbal medicine eight-treasure extract as the main component for sterilizing and diminishing inflammation in the mouthwash, has the effects of sterilizing and diminishing inflammation, is safe and harmless, and can be used for a long time.

(2) Compared with a chemical surfactant, the natural surfactant is selected, so that the safety is higher, and the natural surfactant is more suitable for the consumption field.

(3) The natural surfactant disclosed by the invention is a novel natural surfactant for developing phyllanthus emblica, belongs to innovative utilization of phyllanthus emblica, and fills up the application blank of the phyllanthus emblica processing technology in the field of surfactants.

(4) The natural surfactant disclosed by the invention is good in permeability and strong in foaming power, and overcomes the defect that the surface activity of the conventional natural surfactant is small in the aspects of detergency, dispersivity and the like to a certain extent.

(5) The preparation method of the natural active agent is simple and reliable, has high extraction rate, and can obviously reduce the extraction time compared with the common water extraction or alcohol extraction.

(6) According to the preparation method, the eucommia ulmoides leaf extract is creatively added in the first water extraction process, so that the eucommia ulmoides leaf extract is used as an auxiliary promoter, the extraction time can be shortened, the extraction efficiency is improved, and the prepared natural surfactant has a certain mildew-proof effect. Most importantly, the finally prepared mixture of the emblic leafflower fruit extract and the eucommia ulmoides extract is used as a natural surfactant, and the penetrating action of a single emblic leafflower fruit surfactant can be effectively improved.

Detailed Description

Eight treasures of long-life herb (scientific name: Hylotelephium spectabile (Bor.) H. Ohba) are perennial herbs of the genus eight treasures of the family Crassulaceae. The stem is upright and the height can reach 70 cm. Blade-to-blade, or impeller, oval to wide oval, or oblong oval, with full margin or more or less of undulating teeth. Big inflorescence, chamber shape, terminal growth, dense flowers, sepals are coated with needles to be wide coated with needles in linear shape, petals are coated with purplish red to be purplish red, needles to be wide coated with needles, and anthers are purple; scale rectangle, narrow heart-skin ellipse, gu the whole-length is upright. Blooming in 8-9 months, and bearing fruit in 9-10 months.

Is distributed in Anhui, Shaanxi, Henan, Shandong, Hebei, Liaoning, Jilin and Heilongjiang in China. There is also a distribution in korea. Growing on the multi-stone hillside of the low mountain. The soil is like the environment of sufficient sunlight, warmth, dryness and ventilation, is not wet by water and has no strict requirement on the soil. It is cold-resistant and drought-resistant.

The main values of the eight treasures with long medicine are: the plant cultivation material can be used for arranging flower beds, making net rings, square blocks, cloud rolls, arcs, sectors and the like for landscaping, can also be used as ground cover plants, is tidy in plants, strong in growth and good in group effect, and is a good material for arranging flower beds, decorating lawns and rockyards.

Flowers of the long-medicine eight-treasure are often collected by a doctor in ancient times and dried for later use to make tea, and the effects of clearing heat and reducing internal heat can be achieved. However, the stem and leaf functions of the eight-treasure medicine are rarely researched at present, and the eight-treasure medicine is not used in the mouthwash in any precedent.

Fresh leaves of the Chang Yao eight-treasure are beaten to obtain juice (diluted), and then the Chang Yao eight-treasure extract can be rapidly obtained. The preliminary study shows that the oral cavity and skin inflammation diminishing and breath purifying effects are achieved. In the research process, the alkaline components in the eight-treasure extract of the long-acting medicine can be used as the pH regulator of the mouthwash system, and the using amount and the using type of the additive in the mouthwash can be reduced.

In addition, the platycodon root juice is used for replacing essence and a sweetening agent in the existing mouthwash (the sweetening agent commonly used in the existing mouthwash on the market is saccharin sodium, aspartame, stevioside and sucralose), the components are safer, the number of additives is effectively reduced, and more importantly, the platycodon root juice is compounded with mineral salt, so that the astringent taste of the eight-treasure extract of long-drug ingredients can be effectively neutralized and shielded.

The preservative is one or two of sodium benzoate and nipagin ester.

The humectant is glycerin.

The above preservatives and humectants are all purchased directly.

However, more intensive studies show that the extract of the long-acting medicinal eight-treasure instead of the existing bactericidal component in the mouthwash has the functions of diminishing inflammation of oral skin and purifying oral odor, but the oral cleaning capability is not enough, so that the mouthwash cannot be said to have good performance by using the extract of the long-acting medicinal eight-treasure singly as a functional component.

Based on the above, the invention provides a natural environment-friendly surfactant, which utilizes an extract of emblic leafflower fruit as a surface active functional component. The natural surfactant disclosed by the invention is good in permeability and strong in foaming power, and overcomes the defect that the surface activity of the conventional natural surfactant is small in the aspects of detergency, dispersivity and the like to a certain extent. The surfactant may be compounded with other components to be used as a functional cleaning agent or the like, and the form of the surfactant is not limited to the kind thereof, and may be in the form of powder.

(ii) the above Phyllanthus emblicaPhyllanthus emblica Linn.) Is a plant of Phyllanthus of Euphorbiaceae, which grows in the area where elevation 200-. The root system of the emblic leafflower fruit is developed, the water and the soil can be kept, and the emblic leafflower fruit can be used as a landscape tree in a garden; the tree root and the tree leaf are used for medicine, and can clear away heat and toxic materials and treat dermatitis, eczema, rheumatalgia and the like; sun drying the leaves to obtain the material for pillow core; tannin extract can be extracted from bark, leaf and young fruit; the wood is dark brown, hard, used as agricultural implement and furniture material, and is also an excellent firewood.

It is to be noted that there is no report on the extraction of a mixture of the kernel of emblic leafflower fruit and the pulp of emblic leafflower fruit as the active ingredient of the surfactant.

The invention uses the mixture of the freshly picked emblic leafflower fruit kernel and the emblic leafflower fruit pulp to extract for use, and the extract can endow the surfactant with certain cleaning capability, thereby being the first creation of the emblic leafflower fruit as the liquid surfactant.

The extraction of the emblic extract can be generally carried out by water extraction, leaching, ultrasonic extraction and the like, but the preparation method, in particular the extraction method, is a brand-new extraction method of the emblic extract.

Further explaining, the extraction adopts two extraction methods, wherein in the first step, hot water with the temperature of 80-90 ℃ is vibrated for water extraction, and the eucommia ulmoides leaf extract is added in the water extraction process, and is kept stand and filtered; and (3) for the second time: extracting the mixture of water and alcohol at 15 deg.C, standing, and filtering.

Particularly, the ratio of the materials to the liquid in the first hot water oscillation water extraction is 1: 12-15, time 4-6H; the mixing ratio of the second water and the alcohol is 2-4: 1, the ratio of material to liquid is 1: 10-12 hours and 2-4 hours.

In the comparison test, the tannin can not be precipitated at the normal temperature in the first time and the second time.

More specifically, the second alcohol concentration should not be less than 90 ℃ and the first temperature should not be less than 50 ℃, preferably 80-90 ℃, and the flavone substance should not be cold-soaked.

In addition, the temperature of the hot water extraction in the first step and the temperature of the cold material extraction in the second step have a significant temperature difference gradually, so that the tanning in the emblic leafflower fruit kernels can be mostly precipitated.

It is also critical that the second addition of alcohol ensures that the saponin does not produce secondary glycosides during the extraction process.

It should also be noted that: the eucommia ulmoides leaf extract is selected from old eucommia ulmoides leaves, and the above-mentioned extraction process of the eucommia ulmoides leaf extract is performed according to a method known in the art, and a detailed description thereof will be omitted.

As a key point, the addition of the eucommia ulmoides leaf extract can improve the effective extraction of saponin in the mixture of the emblic leafflower fruit kernel and the emblic leafflower fruit pulp, otherwise, the surface active ingredient yield is low, and the whole research result is influenced.

The technical proposal ensures the yield of the saponin active ingredients in the mixture of the oil orange kernels and the oil orange pulp together.

The traditional water extraction or alcohol extraction is carried out for more than 10 hours, and more than 24 hours, which is obviously different from the extraction time of the invention.

Meanwhile, the mixture of the emblic leafflower fruit kernel and the emblic leafflower fruit pulp is selected, so that more active ingredients can be obtained in a lowest cost mode.

The above-mentioned liquid is prepared, and is subjected to high-pressure concentration and freeze drying, or dried for use, and if dried for use, 10-20 times of the amount of the extraction solvent is added.

The addition amount of the folium cortex eucommiae extract is 0.5-2% of the total weight of the feed liquid, if the content is less than 0.5% of the total weight, the effect is weak, and if the folium cortex eucommiae extract is added in more, the problem of unstable active ingredients can be caused.

The surfactant of the present invention is particularly useful for cleaning fruits and vegetables because of its inherent non-toxicity and innocuousness. Any formulation agent conventional in the art can be prepared, and the field of use thereof is not limited, for example, a toilet detergent, a furniture detergent, and the like.

The surfactant prepared by the invention has the advantages of high purity, light color, no special odor, strong and durable foaming force, and can fill the blank of natural surfactant products of emblic leafflower fruits on the market.

Hereinafter, the present invention will be described in more detail by the following experiments. These examples are intended to be illustrative of the invention and the scope of the invention is not limited thereto.

In the following examples, bacteriostatic mouth washes were prepared by the following steps:

step S1, preparing a natural surfactant;

step 1: preparing an extract of folium cortex eucommiae;

step 2: preparing an emblic leafflower fruit extract: mixing fresh fructus Phyllanthi kernel and fructus Phyllanthi pulp, mincing, extracting with solvent for several times, and adding a certain amount of folium Eucommiae extract during the solvent extraction;

and step 3: mixing filtrates obtained after the solvent is extracted for multiple times, standing, filtering and concentrating to a small volume to obtain a natural surfactant;

step two, S2, preparing bacteriostatic mouth wash;

step S1, putting deionized water into a clean container, adding the preservative (sorbic acid is selected), the humectant (glycerin is selected) and mineral salt while stirring (500 r/min by using a magnetic stirrer) at room temperature until the preservative (sorbic acid is selected), the humectant (glycerin is selected) and the mineral salt are completely dissolved and uniformly dispersed to prepare phase A; step S2, adding the eight-treasure long-medicine extract, the platycodon root juice and the natural surfactant into the phase A, and stirring uniformly at room temperature until a stable clear solution system is formed; and step S3, standing at room temperature for a preset time, filtering, adding deionized water, and homogenizing and performing aseptic treatment to obtain the antibacterial mouthwash.

In the invention, a solubilizer (polysorbate is selected), a flavoring agent (mint is selected), and an organic silicon defoaming agent can be optionally added or not and are not used as essential components. For example, mint flavors may be added to enhance the cooling sensation of the oral cavity.

The eight-treasure extract of long-drug and the natural surfactant used in the process are both independently researched and developed by the inventor, and other reagents are purchased by common commercial approaches.

The preparation process of the eight-treasure extract of the long medicine is as follows: weighing 100g of fresh stem leaves of the Chang Yao eight-treasure, mashing and grinding the stem leaves in a mortar, adding pure water according to the liquid-solid ratio of 6:1, shaking up, sealing, placing the mixture in a dark place indoors, soaking for 12h, carrying out ultrasound for 5min every 4 hours for 3 times, mixing the obtained leachate, and carrying out reduced pressure recovery and filtration to obtain the Chang Yao eight-treasure extract, wherein the finished product is light green.

Through multiple process verification, the optimal process parameters of the mouthwash disclosed by the invention are as follows: 8% of long-medicine eight-treasure extract, 0.2% of platycodon root juice, 3% of humectant, 0.05% of preservative, 0.2% of mineral salt, 11% of natural surfactant and the balance of deionized water.

The optimal preparation process conditions of the natural surfactant are as follows:

(1) drying and crushing eucommia leaves, extracting the eucommia leaves with water for three times, wherein the mass of the water used in each time is 10 times, 10 times and 9 times of that of the eucommia leaves, the reflux time of each extraction is 2 hours, l.5hours and lh in sequence, filtering the three extracting solutions, combining the filtrates, and concentrating to a small volume to obtain the eucommia leaf extract.

(2) Mixing fresh fructus Phyllanthi kernel and fructus Phyllanthi pulp (the ratio of the kernel to the pulp is 1: 3), mincing to 80 mesh, weighing 200g, extracting with 2400ml of 80 deg.C hot water for the first time, shaking for 10min, adding 13g of folium Eucommiae extract during shaking, standing for 6h, and filtering to obtain filtrate and residue. And continuously adding filter residues into a mixture of water and alcohol, wherein the mixing ratio of the water to the alcohol is 4: 1, final alcohol concentration is 90 degrees, the temperature of the mixture is cooled to about 15 ℃, and the second step of extraction is carried out, wherein the ratio of materials to liquid is 1: and 10, standing for 4 hours.

(3) And combining the filtrates obtained in the two steps, standing, filtering and concentrating to a small volume to obtain a natural surfactant serving as a solution to be detected for later use.

Adding 100ml of solution to be detected into a vector cup by adopting a Waring-Blender method, stirring at a constant speed for 60 s during the test, stopping stirring, recording the volume V0 of the generated foam as 845.9ml, and recording the time t required for discharging 50ml of liquid in the foam as 267s

In addition, the preparation process of the bacteriostatic mouth wash is mainly a physical mixing process, so that the content ratio of each component in the finally obtained solution is equal to the addition amount of the components. In the following description, the addition amounts of the respective components are expressed by percentage contents, and these percentage contents refer to weight percentage contents. Obviously, these contents also correspond to the contents of the ingredients in the finished mouthwash solution in percentages by mass.

Example 1

In the embodiment, the formula of the mouthwash comprises 5% of eight-treasure extract of long medicine, 0.1-0.5% of platycodon root juice, 3% of humectant, 0.01% of preservative, 0.1% of mineral salt, 2% of natural surfactant and the balance of deionized water.

Example 2

In the embodiment, the formula of the mouthwash comprises 10% of eight-treasure extract of long medicine, 0.5% of platycodon root juice, 5% of humectant, 0.1% of preservative, 0.5% of mineral salt, 20% of natural surfactant and the balance of deionized water.

Example 3

In the embodiment, the formula of the mouthwash comprises 8% of eight-treasure extract of long medicine, 0.2% of platycodon root juice, 3% of humectant, 0.05% of preservative, 0.2% of mineral salt, 11% of natural surfactant and the balance of deionized water.

Example 4

In the embodiment, the formula of the mouthwash comprises 6% of eight-treasure extract of long medicine, 0.2% of platycodon root juice, 4% of humectant, 0.02% of preservative, 0.4% of mineral salt, 15% of natural surfactant and the balance of deionized water.

Example 5

In this example, the formulation of the mouthwash is:

8% of long-medicine eight-treasure extract, 0.2% of platycodon root juice, 3% of humectant, 0.05% of preservative, 0.2% of mineral salt, 0.1% of polysorbate solubilizer, 1.0% of mint, 0.02% of organic silicon defoamer, 11% of natural surfactant and the balance of deionized water.

The following comparative examples are comparative experiments with the bacteriostatic mouth rinse of the invention:

comparative example 1

In the comparative example, the formula of the mouthwash comprises 6% of the eight-treasure extract of the long medicine, 4% of the humectant, 0.02% of the preservative, 0.4% of the mineral salt, 15% of the natural surfactant and the balance of deionized water.

The procedure was as in example 4.

Comparative example 2

In this comparative example, a mouthwash was prepared according to the same procedure as in example 3. The formula comprises 8 percent of eight-treasure extract of long-medicine, 0.2 percent of platycodon root juice, 3 percent of humectant, 0.05 percent of preservative, 0.2 percent of mineral salt, 15 percent of natural surfactant of sucrose fatty acid ester and the balance of deionized water.

Comparative example 3

In the comparative example, the formula of the mouthwash is 6% of chlorhexidine gluconate, 0.2% of platycodon root juice, 4% of humectant, 0.02% of preservative, 0.4% of mineral salt, 15% of natural surfactant and the balance of deionized water.

Comparative example 4

In the comparative example, the formula of the mouthwash comprises 15% of eight-treasure extract of long medicine, 0.1% of platycodon root juice, 1% of humectant, 0.2% of preservative, 0.01% of mineral salt, 1% of natural surfactant and the balance of water.

Comparative example 5

In the comparative example, the formula of the mouthwash is 6% of chlorhexidine gluconate, 4% of humectant, 0.02% of preservative, 0.4% of mineral salt, 15% of natural surfactant and the balance of deionized water.

The effects of the above examples and comparative examples are illustrated.

1. And (5) performing bacteriostatic experiments.

According to the method of appendix C4 in GB15797-2002 hygienic Standard for Disposable sanitary articles, the bactericidal capacity of the bactericidal composition on staphylococcus aureus (ATCC6538) and escherichia coli (ATCC 25922) is detected by a suspension quantitative method, the detection concentration is 1:1, the action time is 10min, and the result is shown in the following table.

Group of	Staphylococcus aureus bacteriostasis rate	Antibacterial rate of Escherichia coli	Group of	Staphylococcus aureus bacteriostasis rate	Antibacterial rate of Escherichia coli
						Example 1	98.04%	98.74%	Comparative example 3	94.99%	93.23%
Example 2	97.68%	97.69%	Comparative example 4	97.12%	97.45%
						Example 3	98.92%	99.79%	Comparative example 2	97.64%	97.02%
Example 4	97.47%	97.53%	Comparative example 1	96.11%	96.89%
						Example 5	98.04%	98.55%	Comparative example 5	94.85%	93.44%

Note: the above results are compared laterally to illustrate the effect.

From the above table the following conclusions can be drawn:

(1) the bacteriostatic mouth wash of example 1-example 5 has good bacteriostatic effect on both bacteria, as the best effect of example 3.

(2) As can be seen from the comparison between example 1 and comparative example 3, the bacteriostatic effect of chlorhexidine gluconate is inferior to that of the eight-treasure extract of radix salviae miltiorrhizae of example 1.

(3) As can be seen from the comparison between example 2 and comparative example 4, increasing the amount of each component did not significantly improve the bacteriostatic effect of the mouthwash against both bacteria.

(4) From a comparison of example 3 and comparative example 2, it can be seen that other natural surfactants were used

The mouthwash has slightly different antibacterial effects compared with the natural surfactant, but the natural surfactant has better decontamination effect, and the experiments are specifically performed below.

(5) From the comparison of example 4 and comparative example 1, it can be seen that when no platycodon root juice is added, the bacteriostatic effect of the mouthwash is obviously reduced, and through further research, from the comparison of comparative example 3 and comparative example 5, it can be seen that when the platycodon root juice is added, the bacteriostatic effect of the chlorhexidine gluconate mouthwash is not obviously improved. The inventor tentatively thinks that balloonflower juice itself does not have bactericidal effect, and the promotion of antibacterial effect when adding balloonflower juice is because balloonflower juice can promote the anti-inflammatory bacteriostasis of the eight treasures of long medicine for when both used jointly, the antibacterial effect of mouthwash obtains promoting. And the promoting effect can be only shown when the eight-treasure extract of the long medicine is compounded with the platycodon root juice. In addition, the alkaline components in the eight-treasure medicine for growing medicines can regulate and control the pH value of the mouthwash system, so that the osmotic pressure is changed, and bacteria can not maintain the homeostasis and die. Further mechanisms remain to be studied.

(6) Therefore, from the current research, in order to maintain the obvious antibacterial effect and the oral cavity cleaning effect of the mouthwash of the invention, the natural surfactants of the eight-treasure medicine, the platycodon root juice and the emblic leafflower fruit are not necessary, and the common antibacterial agent does not play the effect of the invention.

2. Irritation test

45 college students who have healthy tissues without oral diseases, are 18-26 years old and half of all male and female students are used as volunteers, and are randomly divided into 3 groups of 15 people, the mouth wash prepared in the embodiment 1-5 is used for 5 days, and the mouth wash is used in the morning and the evening every day.

The following are the results of comparative tests of the natural surfactant of the present invention and common bacteriostatic agents:

the preparation method of the natural surfactant comprises the following steps of;

(1) drying and crushing eucommia leaves, extracting the eucommia leaves with water for three times, wherein the mass of the water used in each time is 10 times, 10 times and 9 times of that of the eucommia leaves, the reflux time of each extraction is 2 hours, l.5hours and lh in sequence, filtering the three extracting solutions, combining the filtrates, and concentrating to a small volume to obtain the eucommia leaf extract.

(2) Mixing fresh fructus Phyllanthi kernel and fructus Phyllanthi pulp (the ratio of the kernel to the pulp is 1: 3), mincing to 80 mesh, weighing 200g, extracting with 2400ml of 80 deg.C hot water for the first time, shaking for 10min, adding 13g of folium Eucommiae extract during shaking, standing for 6h, and filtering to obtain filtrate and residue. And (3) continuously adding filter residues into a mixture of water and alcohol, wherein the temperature of the mixture is about 15 ℃, and performing second-step extraction, wherein the material-liquid ratio is 1: and 10, standing for 2 hours.

(3) And combining the filtrates obtained in the two steps, standing, filtering and concentrating to a small volume to obtain a natural surfactant serving as a solution to be detected for later use.

When the color of the filtrate is darker, the filtrate can be decolorized by passing through a treated activated carbon column, and the pressure of the eluent is continuously reduced.

And (3) adding 100ml of solution to be detected into the vector cup by adopting a Waring-Blender method, stirring at a constant speed for 60 s during the test, stopping stirring, and recording the volume of the generated foam. Compared with the common natural surfactant of sucrose fatty acid ester with air bubble performance by adopting the method: the results show that V0 is 805.9ml, V0 'is 744.9ml, the time t required to expel 50ml of liquid in the foam is 244s, and t' is 212 s.

Claims (9)

1. The bacteriostatic mouth wash is characterized by comprising the following components in percentage by weight: 5-10% of long-acting eight-treasure extract, 0.1-0.5% of platycodon root juice, 3-5% of humectant, 0.01-0.1% of preservative, 0.1-0.5% of mineral salt, 2-20% of natural surfactant and the balance of deionized water;

the antibacterial mouthwash is prepared according to the following steps:

step S1, preparing a natural surfactant;

step 1: preparing an extract of folium cortex eucommiae;

step 2: preparing an emblic leafflower fruit extract: mixing fresh fructus Phyllanthi kernel and fructus Phyllanthi pulp, mincing, extracting with solvent for several times, and adding a certain amount of folium Eucommiae extract during the solvent extraction;

and step 3: mixing filtrates obtained after the solvent is extracted for multiple times, standing, filtering and concentrating to a small volume to obtain a natural surfactant;

step two, S2, preparing bacteriostatic mouth wash;

putting deionized water into a clean container, adding the preservative, the humectant and the mineral salt while stirring at room temperature until the preservative, the humectant and the mineral salt are completely dissolved and uniformly dispersed to prepare a phase A;

adding the eight-treasure long-drug extract, the platycodon root juice and the natural surfactant into the phase A, and stirring uniformly at room temperature until a stable clear solution system is formed;

standing the mixture at room temperature for a preset time, filtering, adding deionized water, and homogenizing to obtain the antibacterial mouthwash.

2. The bacteriostatic mouth wash according to claim 1, which comprises the following components in percentage by weight: 7 to 9 percent of long-medicine eight-treasure extract, 0.1 to 0.3 percent of platycodon root juice, 3 to 5 percent of humectant, 0.01 to 0.1 percent of preservative, 0.1 to 0.5 percent of mineral salt, 2 to 15 percent of natural surfactant and the balance of deionized water.

3. The bacteriostatic mouth wash according to claim 2, which comprises the following components in percentage by weight: 8% of long-medicine eight-treasure extract, 0.2% of platycodon root juice, 3% of humectant, 0.05% of preservative, 0.2% of mineral salt, 11% of natural surfactant and the balance of deionized water.

4. The bacteriostatic mouthwash according to claim 1, further comprising 0.1 to 0.3 weight percent of a solubilizer.

5. The bacteriostatic mouthwash according to claim 1, further comprising 1.0-3.0% of a flavoring agent by weight.

6. The bacteriostatic mouthwash according to claim 1, further comprising 0.02 to 0.1 weight percent of an organosilicon antifoaming agent.

7. The bacteriostatic mouthwash according to claim 1, wherein the natural surfactant comprises an extract of emblic leafflower fruit and an extract of eucommia ulmoides leaves;

the source of the emblic extract comprises a mixture of fresh emblic kernels and emblic pulps.

8. The method for preparing bacteriostatic mouth wash according to claim 7, wherein the solvent multiple extraction in step one comprises:

for the first time: performing shaking water extraction with hot water, adding folium Eucommiae extract during water extraction, standing, and filtering;

and (3) for the second time: extracting the first residue with mixture of water and alcohol, standing, and filtering.

9. The preparation method of the bacteriostatic mouth wash according to claim 8, wherein the solvent is extracted for a plurality of times, and the method specifically comprises the following steps:

for the first time: shaking with hot water at 80-90 deg.C for water extraction, adding folium Eucommiae extract during water extraction, standing, and filtering;

and (3) for the second time: extracting the mixture of water and alcohol at 15 deg.C, standing, and filtering.