CN112980701A - 促进铁皮石斛种子萌发及生长的复合菌剂 - Google Patents
促进铁皮石斛种子萌发及生长的复合菌剂 Download PDFInfo
- Publication number
- CN112980701A CN112980701A CN202110415077.5A CN202110415077A CN112980701A CN 112980701 A CN112980701 A CN 112980701A CN 202110415077 A CN202110415077 A CN 202110415077A CN 112980701 A CN112980701 A CN 112980701A
- Authority
- CN
- China
- Prior art keywords
- fungus
- dendrobium officinale
- microbial inoculum
- growth
- dendrobium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 25
- 239000002131 composite material Substances 0.000 title claims abstract description 22
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 20
- 230000001737 promoting effect Effects 0.000 title claims abstract description 17
- 230000035784 germination Effects 0.000 title claims abstract description 11
- 241000233866 Fungi Species 0.000 claims abstract description 58
- 230000001580 bacterial effect Effects 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 150000001875 compounds Chemical class 0.000 claims abstract description 13
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241000223218 Fusarium Species 0.000 claims abstract description 12
- 241000216654 Armillaria Species 0.000 claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 241000222418 Lentinus Species 0.000 claims abstract description 5
- 239000000725 suspension Substances 0.000 claims description 12
- 241000574106 Tulasnella sp. Species 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 5
- 241000235395 Mucor Species 0.000 claims description 4
- 241000122904 Mucuna Species 0.000 claims description 2
- 239000003085 diluting agent Substances 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 241001523681 Dendrobium Species 0.000 abstract description 11
- 230000000052 comparative effect Effects 0.000 description 9
- 230000004083 survival effect Effects 0.000 description 8
- 241001149959 Fusarium sp. Species 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000617791 Armillaria sp. Species 0.000 description 4
- 241000989278 Mycena sp. Species 0.000 description 4
- 241000233855 Orchidaceae Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241000088530 Chaetomium sp. Species 0.000 description 3
- 241001361634 Rhizoctonia Species 0.000 description 3
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000222435 Lentinula Species 0.000 description 2
- 241000222350 Pleurotus Species 0.000 description 2
- 241000235527 Rhizopus Species 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 101710186708 Agglutinin Proteins 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 241000026010 Dendrobium candidum Species 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 241001534813 Hypsizygus Species 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 241000131430 Mycena Species 0.000 description 1
- 241000131448 Mycosphaerella Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001522129 Pinellia Species 0.000 description 1
- 241000187433 Streptomyces clavuligerus Species 0.000 description 1
- 241000221365 Tremellales Species 0.000 description 1
- 241000221841 Verticillium sp. (in: Hypocreales) Species 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000002449 bone cell Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种促进铁皮石斛种子萌发及生长的复合菌剂,包括由胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillaria sp.)真菌组成的复合菌液;在复合菌液加入5%PTA silkworm‑P4,得复合菌剂。采用本发明的复合菌剂既能促进石斛种子的萌发,并形成具有菌根的石斛苗,从而促进石斛生长。
Description
技术领域
本发明涉及生物菌肥领域,尤其是一种促进铁皮石斛种子萌发及生长的复合菌剂。
背景技术
铁皮石斛是兰科石斛属多年生附生植物,在医药领域的研究与开发方面应用广泛。铁皮石斛是我国传统名贵中药,为中华“九大仙草”之首,具有益胃生津、滋阴清热的功效。现代医学研究表明,铁皮石斛可有效增强机体免疫力,在抗癌、抗氧化、抗衰老、抗疲劳、促消化、降血糖及促进骨细胞增殖方面具有明显作用。当前由于铁皮石斛生长缓慢,对环境条件要求较严格,产区药农长期采挖等因素导致自然资源已濒临灭绝。
铁皮石斛种子细小且无胚乳,自然条件下必须与特定真菌种共生才能萌发。当前,石斛组织培养体系已成功建立,可大量繁殖石斛无性苗,但无性苗根粗大,根毛不发达,不能满足植物对水分和营养物质的需求,因而移栽成活率低、生长缓慢、生长周期长等问题,此外,组培培养的无性苗在药用价值上比之野生种差异甚大。因而,如何用自然手段诱导石斛种子萌发,培养出与野生石斛苗相似品质的人工种具有重大意义。
现有的铁皮石斛的培养方法有:
1、专利《一种铁皮石斛的复合菌剂》(CN 106497838 A)中告知该复合菌剂为促生放线菌克兰斯基链霉菌与供试铁皮石斛促生菌根真菌瘤菌根菌属和胶膜菌属按照1:1:1的比例配比获得。
2、专利《一种铁皮石斛栽培种植菌根化生产方法》(CN 102150615 A)中告知该采用固体或液体培养基培养菌根真菌,菌根真菌为菌根真菌LP2半知菌亚门镰刀菌属(Fusariumsp.)、菌根真菌LP3半知菌亚门轮枝霉属(Verticilliumsp.)或两种的混合菌株。
3、专利《促进兰科植物种子萌发的三种真菌菌株》(CN 101565676 A)中告知三种真菌菌株为丝核菌属真菌、胶膜菌属真菌和小菇属真菌。
4、论文《兰科菌根共生效应研究进展》中告知小菇属、镰刀菌属和蜜环菌属的真菌对兰科植物的萌发具有促进作用。
发明内容
本发明要解决的技术问题是提供一种铁皮石斛的复合菌剂,采用本发明的复合菌剂即能促进石斛种子的萌发,并形成具有菌根的石斛苗,从而促进石斛生长。
为了解决上述技术问题,本发明提供一种铁皮石斛复合菌剂,包括由胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillaria sp.)真菌组成的复合菌液;在复合菌液加入5%PTA silkworm-P4。即,每100ml的复合菌液中加入5g的PTA silkworm-P4。
作为本发明的铁皮石斛复合菌剂的改进:将胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillaria sp.)真菌分别制成浓度为30~50CFU/mL的悬稀释液;将4种悬稀释液等体积比混合,得复合菌液。
本发明所述的PTA silkworm-P4,在专利号201110069545.4的发明《家蚕反应器高效表达三叶半夏凝集素》中有告知。
铁皮石斛存活难在于1年小苗容易感染有害细菌,造成烂根。本发明通过选用合适的真菌组成复合菌液,并配合适宜配比浓度,且加入PTA silkworm-P4作为有害细菌抑制剂防止有害细菌感染,达到既促进生长又防止小苗根部污染腐烂的目的,具有促进生长,防止烂根的优点,本发明有利于石斛苗尤其是一年小苗的生长,即可促进生长又可以防止烂根,提高一年苗存活率。
具体实施方式
下面结合具体实施例对本发明进行进一步描述,但本发明的保护范围并不仅限于此。
实施例1、铁皮石斛的复合菌剂,包含胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillaria sp.)真菌。以上菌种均能通过常规市购的形式获得,例如可购自山东拓普生物工程有限公司:M4103的胶膜菌属(Tulasnella sp.)真菌、M4112的镰刀菌属(Fusarium sp.)真菌,M4160的小菇属(Mycena sp.)真菌,M4173的蜜环菌属(Armillaria sp.)真菌。
将上述胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillaria sp.)真菌分别制成浓度为30CFU/mL悬稀释液。再按1:1:1:1的体积比混合形成复合菌液,最后加入5%PTA silkworm-P4混合。即,每100ml的复合菌液中加入5g的PTA silkworm-P4。
PTA silkworm-P4对有害细菌有抑制作用。
对比例1-1、取消实施例1中蜜环菌属(Armillaria sp.)真菌,并将实施例1中“镰刀菌属(Fusarium sp.)真菌”更改为“瘤菌根菌属真菌”,“小菇属(Mycena sp.)真菌”更改为“克兰斯基链霉菌”,即,将胶膜菌属(Tulasnella sp.)真菌、瘤菌根菌属真菌和克兰斯基链霉菌分别制成浓度为30CFU/mL悬稀释液。再按1:1:1的体积比混合形成复合菌液,最后加入5%PTA silkworm-P4混合。
对比例1-2、取消实施例1中蜜环菌属(Armillaria sp.)真菌和胶膜菌属(Tulasnella sp.)真菌,并将“小菇属(Mycena sp.)真菌”更改为“轮枝霉属(Verticilliumsp.)真菌”,即,将镰刀菌属(Fusarium sp.)真菌和轮枝霉属(Verticilliumsp.)真菌分别制成浓度为30CFU/mL悬稀释液。再按1:1的体积比混合形成复合菌液,最后加入5%PTA silkworm-P4混合。
对比例1-3、取消实施例1中蜜环菌属(Armillaria sp.)真菌,并将实施例1中“镰刀菌属(Fusarium sp.)真菌”更改为“丝核菌属真菌”,即,将胶膜菌属(Tulasnella sp.)真菌、丝核菌属真菌和小菇属(Mycena sp.)真菌分别制成浓度为30CFU/mL悬稀释液。再按照1:1:1的体积比混合形成复合菌液;最后加入5%PTA silkworm-P4混合。
对比例1-4、将实施例1“蜜环菌属(Armillaria sp.)真菌”更改为“毛壳菌属(Chaetomium sp.)”,即,胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和毛壳菌属(Chaetomium sp.)分别制成浓度为30CFU/mL悬稀释液。再按1:1:1:1的体积比混合形成复合菌液;最后加入5%PTA silkworm-P4混合。
实验1、利用上述实施例1所得复合菌剂促进铁皮石斛种子于培养基中萌发,具体步骤如下:
在无菌条件下,取0.1ml复合菌剂于200ml燕麦培养基(常规培养基)中;按照常规方式将表面消毒后的铁皮石斛种子加入少量无菌水,制成种子悬浮液,吸取50ul的种子悬浮液均匀地涂抹在燕麦培养基上,先25℃暗培养7d后,再室内自然光条件下培养至菌根生成(带疣状突起的根长度超过15mm,菌根上带有5mm菌块),此室内自然光条件下的培养一般需要14天左右。最后可按照常规方法进行移栽和种植。
对比例1-1~对比例1-4所得的复合菌剂按照上述实验1所述方法进行,所得结果与本发明实施例1所得复合菌剂的对比,如下表1所示:
表1
注:空白对照即将0.1ml复合菌剂更改为0.1ml纯净水。成活率为移栽后的一年苗存活率。
实验2、利用实施例1所得复合菌剂促进铁皮石斛种子于自然条件下萌发,具体步骤如下:
将木屑用沸水煮1h后,转移到用沸水浸泡过的干净花盆中,置于室温(25℃);用复合菌剂将木屑浇透,按照常规方式撒入铁皮石斛种子;先放入暗处培养10~15d,并保证木屑湿度在80%~95%之间;再自然条件下培养至菌根(带疣状突起的根长度超过15mm,菌根上带有5mm菌块)生成,最后可按照常规方法进行移栽和种植。
对比例1-1~对比例1-4所得的复合菌剂按照上述实验2所述方法进行,所得结果与本发明实施例1所得复合菌剂的对比,如下表2所示:
表2
注:空白对照即将复合菌剂更改为纯净水。成活率为移栽后的一年苗存活率。
实验3、利用实施例1所得复合菌剂促进铁皮石斛组培苗形成菌根,具体步骤如下:
在无菌条件下,取0.1ml复合菌剂加入至200mlDE共生培养基中,同时接入3~4株铁皮石斛组培苗,于25℃、12h、2000lx光照条件下培养至菌根生成(带疣状突起的根长度超过15mm,菌根上带有5mm菌块),培养时间至少需要1星期;最后可按照常规方法进行移栽和种植。移栽后的成活率基本能达到99%。
对比例1-1~对比例1-4所得的复合菌剂按照上述实验3所述方法进行,在相同的培养时间下,菌根上不能形成有效的菌块,且,移栽后的成活率也远不如本发明(4个对比例中属于效果最佳的对比例1-4,成活率也不到88%)。
最后,还需要注意的是,以上列举的仅是本发明的若干个具体实施例。显然,本发明不限于以上实施例,还可以有许多变形。本领域的普通技术人员能从本发明公开的内容直接导出或联想到的所有变形,均应认为是本发明的保护范围。
Claims (2)
1.促进铁皮石斛种子萌发及生长的复合菌剂,其特征是:包括由胶膜菌属(Tulasnellasp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillaria sp.)真菌组成的复合菌液;在复合菌液加入5%PTA silkworm-P4,得复合菌剂。
2.根据权利要求1所述的铁皮石斛复合菌剂,其特征是:将胶膜菌属(Tulasnella sp.)真菌、镰刀菌属(Fusarium sp.)真菌、小菇属(Mycena sp.)真菌和蜜环菌属(Armillariasp.)真菌分别制成浓度为30~50CFU/mL的悬稀释液;将4种悬稀释液等体积比混合,得复合菌液。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110415077.5A CN112980701A (zh) | 2021-04-17 | 2021-04-17 | 促进铁皮石斛种子萌发及生长的复合菌剂 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110415077.5A CN112980701A (zh) | 2021-04-17 | 2021-04-17 | 促进铁皮石斛种子萌发及生长的复合菌剂 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112980701A true CN112980701A (zh) | 2021-06-18 |
Family
ID=76340960
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110415077.5A Pending CN112980701A (zh) | 2021-04-17 | 2021-04-17 | 促进铁皮石斛种子萌发及生长的复合菌剂 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112980701A (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114342958A (zh) * | 2021-12-31 | 2022-04-15 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | 一种促进兰花种子萌发的拌种菌剂及拌种和培养方法 |
CN114395486A (zh) * | 2022-01-11 | 2022-04-26 | 云南大学 | 一株具有促进石斛株高生长能力的胶膜菌菌株tp-3及其应用 |
CN114395485A (zh) * | 2022-01-11 | 2022-04-26 | 云南大学 | 一种具有促进石斛茎粗生长的胶膜菌菌株tp-2和应用 |
CN114806887A (zh) * | 2022-03-28 | 2022-07-29 | 云南大学 | 一株促进金沙江石斛萌发的真菌菌株和应用 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101565676A (zh) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | 促进兰科植物种子萌发的三株真菌菌株 |
CN102199608A (zh) * | 2011-03-18 | 2011-09-28 | 浙江理工大学 | 家蚕反应器高效表达三叶半夏凝集素 |
CN106497838A (zh) * | 2016-10-28 | 2017-03-15 | 云南山里红生物科技有限公司 | 一种铁皮石斛的复合菌剂 |
CN109793010A (zh) * | 2019-03-14 | 2019-05-24 | 华侨大学 | 一种促进铁皮石斛生长的液体复合菌剂及其使用方法 |
US20200281214A1 (en) * | 2017-09-30 | 2020-09-10 | JlANGSU VOCATIONAL COLLEGE OF AGRICULTURE AND FORESTRY | Fusarium solani and use of same in prevention and treatment of southern blight of dendrobium officinale kimura et migo |
CN111876336A (zh) * | 2020-08-11 | 2020-11-03 | 云南大学 | 胶膜菌属真菌及其促进白旗兜兰种子萌发形成幼苗的应用 |
-
2021
- 2021-04-17 CN CN202110415077.5A patent/CN112980701A/zh active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101565676A (zh) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | 促进兰科植物种子萌发的三株真菌菌株 |
CN102199608A (zh) * | 2011-03-18 | 2011-09-28 | 浙江理工大学 | 家蚕反应器高效表达三叶半夏凝集素 |
CN106497838A (zh) * | 2016-10-28 | 2017-03-15 | 云南山里红生物科技有限公司 | 一种铁皮石斛的复合菌剂 |
US20200281214A1 (en) * | 2017-09-30 | 2020-09-10 | JlANGSU VOCATIONAL COLLEGE OF AGRICULTURE AND FORESTRY | Fusarium solani and use of same in prevention and treatment of southern blight of dendrobium officinale kimura et migo |
CN109793010A (zh) * | 2019-03-14 | 2019-05-24 | 华侨大学 | 一种促进铁皮石斛生长的液体复合菌剂及其使用方法 |
CN111876336A (zh) * | 2020-08-11 | 2020-11-03 | 云南大学 | 胶膜菌属真菌及其促进白旗兜兰种子萌发形成幼苗的应用 |
Non-Patent Citations (3)
Title |
---|
XU LING-LING ET AL.: ""Tulasnellaceae associated with orchids: taxonomy, diversity, specificity and plasticity"", 《MYCOSYSTEMA》 * |
王珊珊 等: ""药用石斛内生菌的研究进展"", 《中国食物与营养》 * |
马琛 等: ""植物凝集素抗虫性研究"", 《现代园艺》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114342958A (zh) * | 2021-12-31 | 2022-04-15 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | 一种促进兰花种子萌发的拌种菌剂及拌种和培养方法 |
CN114395486A (zh) * | 2022-01-11 | 2022-04-26 | 云南大学 | 一株具有促进石斛株高生长能力的胶膜菌菌株tp-3及其应用 |
CN114395485A (zh) * | 2022-01-11 | 2022-04-26 | 云南大学 | 一种具有促进石斛茎粗生长的胶膜菌菌株tp-2和应用 |
CN114395485B (zh) * | 2022-01-11 | 2023-06-23 | 云南大学 | 一种具有促进石斛茎粗的胶膜菌菌株tp-2和应用 |
CN114806887A (zh) * | 2022-03-28 | 2022-07-29 | 云南大学 | 一株促进金沙江石斛萌发的真菌菌株和应用 |
CN114806887B (zh) * | 2022-03-28 | 2023-09-05 | 云南大学 | 一株促进金沙江石斛萌发的真菌菌株和应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112980701A (zh) | 促进铁皮石斛种子萌发及生长的复合菌剂 | |
CN102668878A (zh) | 以莞香木屑食用菌培养基栽培金针菇的方法 | |
CN104145716B (zh) | 硬皮马勃菌根苗的合成方法 | |
CN106416744A (zh) | 一种天麻共生蜜环菌菌种及其制备方法 | |
CN109168958B (zh) | 一种桑黄栽培方法 | |
CN115340968B (zh) | 微刺假单胞菌的新用途及其方法、微刺假单胞菌21 4.1 9.2-14及其产品 | |
KR100823541B1 (ko) | 꽃송이버섯 재배방법 | |
CN108410741A (zh) | 一种柑橘专性am接种剂及其制备方法 | |
KR100483333B1 (ko) | 발효톱밥을 이용한 꽃송이버섯 재배방법 | |
CN113412761B (zh) | 一种工厂化茯苓栽培基质及茯苓栽培方法 | |
CN107629966A (zh) | 真菌诱导子、其制备方法及利用该真菌诱导子进行白芨种苗快繁的方法 | |
KR100431924B1 (ko) | 음식물 쓰레기를 이용한 식용버섯의 재배방법 | |
CN104186201A (zh) | 硬皮马勃人工栽培方法 | |
CN101451112B (zh) | 一种防治果树根癌病的土壤杆菌及其菌剂和制备方法 | |
CN111849856A (zh) | P.indica厚垣孢子、P.indica孢子菌剂及其制备方法 | |
CN108002877B (zh) | 一种利用艾渣栽培平菇的培养基及其栽培方法 | |
CN110229757A (zh) | 一株有效促进作物生长的桔绿木霉js84及其研制的生物有机肥 | |
CN112522110B (zh) | 适用于石斛枯萎病的微生物菌剂及其应用 | |
CN111134142B (zh) | 一种利用枯草芽孢杆菌提高怀山药产量的方法 | |
KR20070048636A (ko) | 인삼 사포닌 성분이 함유된 팽이 버섯의 재배방법 | |
CN107787763A (zh) | 一种高出菇率的大球盖菇培养基及大球盖菇栽培方法 | |
Ridgway et al. | Optimising the medium for producing arbuscular mycorrhizal spores and the effect of inoculation on grapevine growth | |
KR101057500B1 (ko) | 유황 함유량이 높은 느타리버섯 재배방법 및 그 방법에 의해 재배된 느타리버섯 | |
KR101905677B1 (ko) | 천마의 생장촉진용 천연 액비 | |
CN110972806A (zh) | 朱红硫磺菌菌株培育方法、人工栽培方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210618 |