CN112931528A - Safe disinfectant special for raw fish overwintering culture water body and application thereof - Google Patents
Safe disinfectant special for raw fish overwintering culture water body and application thereof Download PDFInfo
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- CN112931528A CN112931528A CN202110117228.9A CN202110117228A CN112931528A CN 112931528 A CN112931528 A CN 112931528A CN 202110117228 A CN202110117228 A CN 202110117228A CN 112931528 A CN112931528 A CN 112931528A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7024—Esters of saccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/02—Medicinal preparations containing materials or reaction products thereof with undetermined constitution from inanimate materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
- A61K41/0057—Photodynamic therapy with a photosensitizer, i.e. agent able to produce reactive oxygen species upon exposure to light or radiation, e.g. UV or visible light; photocleavage of nucleic acids with an agent
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
Abstract
The invention belongs to the technical field of aquaculture water body disinfection, and discloses a special safe disinfectant for a raw fish overwintering aquaculture water body, which comprises the following components in parts by weight: 30-35 parts of potassium hydrogen persulfate composite salt, 25-30 parts of plant astringent, 5-10 parts of sulfamic acid, 20-25 parts of sodium hexametaphosphate, 0.5-1 part of sodium chloride and 1.5-2 parts of nano zinc oxide, wherein the plant astringent is as follows: the mineral source potassium fulvate and the tannin have the mass ratio of (4-5) to 1 in sequence. The plant astringent provided by the invention can promote the healing effect of the injured epidermis of organisms, and greatly reduce the occurrence of rotten body diseases of raw fish caused by water pollution. The special safe disinfectant for the raw fish overwintering culture water body can effectively prevent and treat the rotten condition of the raw fish during overwintering, has mild action and is safe to culture varieties and aquatic; the disinfectant provided by the invention is safe in components and free of residues, and avoids the stress effect on cultured species and the damage to water body micro-balance.
Description
Technical Field
The invention belongs to the technical field of aquaculture water body disinfection, and particularly discloses a special safe disinfectant for a raw fish overwintering aquaculture water body and application thereof.
Background
During the winter period, the water temperature in the pond is low, which is the epidemic period of disease outbreak such as rotten body and water mould of snakehead (also called 'raw fish'). The root of the disease causing the rotten body of the snakehead is the myceliophthora, and the optimum temperature for the growth of the sporangium of the myceliophthora is 16-24 ℃, so the disease is easy to be outbreak in winter and early spring. Therefore, the easy rotting of the raw fish in the overwintering season is a big problem in the water body culture of the raw fish. Due to the fact that in winter, the temperature is low, organic matters in aquaculture water are deposited, the abundance of algae is poor, after the temperature is warmed, the organic matters are fermented to cause water quality deterioration, the influence of fungus infection such as water mold is superposed, diseases such as rotten bodies of living fishes are easily caused, and therefore the living fishes die greatly, and great loss is caused to farmers. The water quality of the pond is deteriorated, and the fish body surface is injured. Prevention in advance is needed, operations such as water adding or water changing are avoided, and fish body stress, detackification, body surface redness and the like are easily caused due to overlarge water quality change, so that the fish body is descended and ill. The mild water body regulator is suitable for the period, can regulate and control water quality, and avoids fish body stress caused by water quality change in the water body.
At present, no specific medicine is available for the problem of rotten fish bodies caused by fungi such as saprolegnia and the like, so that the method has practical application value for reducing or avoiding the breeding problems, and effective prevention is needed in the early stage of the overwintering period.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide the special safe disinfectant for the living fish overwintering culture water body. The invention provides an environment-friendly disinfectant, which is prepared by reasonably compounding a plant astringent as an auxiliary material. The disinfectant has mild effect, has small stress on zooplankton in the aquaculture water body, does not cause the damage effect of disinfectants such as chlorine preparations on aquatic microecological balance, and can effectively prevent diseases such as the rotten body of overwintering fishes.
The invention also aims to provide application of the special safe disinfectant for the raw fish overwintering culture water body.
The technical purpose of the invention is realized by the following technical scheme:
the safe disinfectant special for the water body for the overwintering culture of the raw fishes comprises the following components in parts by weight: 30-35 parts of potassium hydrogen persulfate composite salt, 25-30 parts of plant astringent, 5-10 parts of sulfamic acid, 20-25 parts of sodium hexametaphosphate, 0.5-1 part of sodium chloride and 1.5-2 parts of nano zinc oxide, wherein the plant astringent is as follows: mineral source potassium fulvate and tannic acid, wherein the mass ratio of (4-5): 1.
as a preferred technical scheme, the particle size of the potassium hydrogen persulfate composite salt, the sulfamic acid and the sodium chloride is 30-80 meshes; the grain size of the mineral potassium fulvate and the tannin is more than 100 meshes and more than or equal to 95 percent.
More preferably, the particle size of the mineral potassium fulvate and tannic acid is 100-200 meshes.
The invention also provides a preparation method of the special safe disinfectant for the raw fish overwintering culture water body, which comprises the following steps:
s1, mixing potassium hydrogen persulfate composite salt with nano zinc oxide;
s2, mixing mineral potassium fulvate with the substances in the step S1;
s3, adding sulfamic acid, sodium hexametaphosphate and sodium chloride into the mixture obtained in the step S2;
s4, finally, the tannic acid and the substances in the step S3 are fully and uniformly mixed and packaged.
According to the invention, the nanometer zinc oxide is firstly utilized to mix the potassium hydrogen persulfate composite salt, so that the potassium hydrogen persulfate composite salt can be wrapped, and the oxidation activity of the potassium hydrogen persulfate composite salt is protected.
The invention protects the application of the special safe disinfectant for the raw fish overwintering culture water body in preparing preparations for preventing and treating the rotten body diseases of the raw fish.
Furthermore, different dosages are adopted according to specific stages, for example, the dosage of the special safe type for the raw fish overwintering culture water body is 0.3-0.5ppm during prevention. When the application is applied to the treatment of the overwintering rotten body of the raw fish, the use amount of the special safe type for the raw fish overwintering culture water body is 0.6-1.0ppm, and the safe type is continuously used for 2-3 days.
By adopting the disinfectant provided by the invention, the rotten condition of the raw fish during overwintering can be effectively prevented and treated, the stability of the product is good, and the product can be maintained for 12 hours at the illumination intensity of 3000lux, the temperature of 45 ℃ and the humidity of 75%; the illumination intensity is 0lux, the temperature is 20 ℃, and the humidity is 40 percent and is maintained for 12 hours; under 2 alternate protocols, after 30 days it was found that: the bactericidal effect of the product can still reach 100 percent, which shows that the product has longer prevention and treatment effects, can keep stable effect in extreme environments, is economic and effective, and is suitable for large-area popularization and use.
Compared with the prior art, the invention has the following beneficial effects:
1. the disinfectant provided by the invention can effectively prevent and treat the rotten condition of the live fish during overwintering, has mild effect and is safe to breeding varieties and aquatic;
2. the disinfectant provided by the invention is safe in components and free of residues, and avoids the stress effect on cultured species and the damage to water body micro-balance;
3. the plant astringent provided by the invention can promote the healing effect of the injured epidermis of organisms, and further synergistically avoid the occurrence of rotten body diseases of raw fishes caused by water body pollution.
Detailed Description
The invention is further illustrated by the following examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Experimental procedures without specific conditions noted in the examples below, generally according to conditions conventional in the art or as suggested by the manufacturer; the raw materials, reagents and the like used are, unless otherwise specified, those commercially available from the conventional markets and the like. Any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
In order that the invention may be more fully understood, reference will now be made to the accompanying examples. The following is a description of the preferred embodiments of the present invention, but is not intended to be limiting.
Example 1
A special safe disinfectant for a raw fish overwintering culture water body is prepared by compounding the following steps:
step a, uniformly mixing 35 parts of potassium hydrogen persulfate composite salt and 1.5 parts of nano zinc oxide;
b.25 parts of mineral source fulvic acid potassium is uniformly mixed with the part a;
c, adding 10 parts of sulfamic acid, 22.5 parts of sodium hexametaphosphate and 1 part of sodium chloride into the mixture b, and uniformly mixing;
d.5, fully and uniformly mixing the tannic acid and the tannic acid; the product is packed in 1 Kg/bag.
The particle size of the potassium hydrogen persulfate composite salt, the particle size of the sulfamic acid and the particle size of the sodium chloride are 30-80 meshes, the particle size of the mineral potassium fulvate and the particle size of the tannin are 100-200 meshes, and the moisture content of the raw materials is less than 3%.
Comparative example 1
A disinfectant is prepared by compounding the following steps:
step a, uniformly mixing 35 parts of potassium hydrogen persulfate composite salt and 1.5 parts of nano zinc oxide;
b.30 parts of zeolite powder and a are mixed uniformly;
c, adding 10 parts of sulfamic acid, 22.5 parts of sodium hexametaphosphate and 1 part of sodium chloride into the mixture b, and uniformly mixing; the product is packed in 1 Kg/bag.
The particle size of the potassium hydrogen persulfate composite salt, the particle size of the sulfamic acid and the particle size of the sodium chloride are 30-80 meshes, and the moisture content of the raw materials is less than 3%.
Comparative example 2
A disinfectant is prepared by compounding the following steps:
step a, uniformly mixing 35 parts of 100% potassium hydrogen persulfate composite salt which is sieved by a 100-mesh sieve with 1.5 parts of nano zinc oxide;
b.25 parts of mineral source fulvic acid potassium is uniformly mixed with the part a;
c, adding 10 parts of sulfamic acid, 22.5 parts of sodium hexametaphosphate and 1 part of sodium chloride into the mixture b, and uniformly mixing;
d.5, fully and uniformly mixing the tannic acid and the tannic acid; the product is packed in 1 Kg/bag.
The particle size of the sulfamic acid and the sodium chloride is 30-80 meshes, the particle size of the mineral potassium fulvate and the mineral tannic acid is 100-200 meshes, and the moisture content of the raw materials is less than 3%.
Comparative example 3
A disinfectant is prepared by compounding the following steps:
step a, uniformly mixing 35 parts of potassium hydrogen persulfate composite salt with 1.5 parts of light magnesium carbonate (more than or equal to 99.5 percent of the light magnesium carbonate sieved by a 100-mesh sieve);
b.25 parts of mineral source fulvic acid potassium is uniformly mixed with the part a;
c, adding 10 parts of sulfamic acid, 22.5 parts of sodium hexametaphosphate and 1 part of sodium chloride into the mixture b, and uniformly mixing;
d.5, fully and uniformly mixing the tannic acid and the tannic acid; the product is packed in 1 Kg/bag.
The particle size of the potassium hydrogen persulfate composite salt, the particle size of the sulfamic acid and the particle size of the sodium chloride are 30-80 meshes, the particle size of the mineral potassium fulvate and the particle size of the tannin are 100-200 meshes, and the moisture content of the raw materials is less than 3%.
Application Experimental example 1
The sea gull island base of the wine, 12 port cement ponds, divide into 4 groups averagely, 3 port cement ponds of each group, serial number A, B, C, D. 480 healthy raw fishes with uniform specifications are fished from ponds with perennial rotten diseases and evenly distributed into cement ponds, 40 fishes in each cement pond are fed uniformly, dead fishes are fished out in time and fed continuously for 3 months.
Test protocol: group A is a control group and is not treated; group B is example 1 schemeGroup (d); group C is comparative example 1; group D is the protocol of example 1, but in an amount 5 times the conventional amount; the use scheme of the product is as follows: 1.0g/m3The water body of (2) is used once every week; and (5) counting the number of the raw fishes and the number and the proportion of the raw fishes with rotten body symptoms at the end. See table 1 for details.
TABLE 1
| Numbering | Residual mantissa (Tail) | Rotten mantissa (tail) | Ratio/%) |
| A (control group) | 110 | 17 | 15.45％ |
| B (example 1 conventional amount) | 118 | 1 | 0.85％ |
| C (comparative example 1) | 118 | 6 | 5.08％ |
| D (example 1 high dose) | 117 | 1 | 0.85％ |
As can be seen from table 1, the formulation provided by the present invention can control the occurrence rate of the rotten body disease of the raw fish within 1.0% by the conventional prevention treatment, the product safety is high, the increase of the death number is not caused by 5 times of the usage amount, and the formulation of comparative example 1 can achieve a certain effect of preventing the rotten body disease of the raw fish, but the effect is not as good as that of the present invention. The compounding of the mineral potassium fulvate and the tannin can better avoid the rotten body disease of the raw fish.
Application Experimental example 2
Culturing and sampling the Shundde raw fishes, evaluating the severity of rotten body diseases by sampling (fishing 30-50 raw fishes at the four corners of the pond respectively, counting the number of the rotten body fishes, calculating the rotten body disease proportion after gathering), and selecting ponds with similar 4 mouth rotten body proportions for product effect evaluation, wherein the pond is numbered A, B, C, D; A. b, using a daily treatment scheme for the pond B, using the disinfectant scheme of the embodiment 1 for the C, D pond for treatment, wherein the dosage is 1.0ppm (namely 400-; see table 2 for details:
TABLE 2
| A (control group) | B | C | D | |
| 1 day | 7.58％ | 6.74％ | 6.90％ | 7.10％ |
| 8 days | 11.17％ | 17.42％ | 6.60％ | 6.67％ |
| 15 days | 12.02％ | 14.97％ | 4.41％ | 7.01％ |
| 22 days | 14.29％ | 22.13％ | 2.96％ | 2.36％ |
| 29 days | 18.03％ | 16.88％ | 2.68％ | 2.78％ |
| 36 days | 19.01％ | 16.45％ | 1.90％ | 2.33％ |
As can be seen from table 2, after the scheme of embodiment 1 of the present invention is adopted for sterilization, the proportion of rotten bodies of the raw fish is low, and the effect can be kept stable in about one month of cultivation period, which indicates that the formula can not only effectively prevent the rotten bodies of the raw fish, but also greatly reduce the rotten bodies of the raw fish, and has the advantages of long prevention and treatment effects, stable effect, economy and effectiveness, and suitability for large-area popularization and use.
Application Experimental example 3
10 packets of samples of example 1, comparative example 2 and comparative example 3 were placed in a drug stability test box with the test box conditions set: the illumination intensity is 3000lux, the temperature is 45 ℃, and the humidity is 75 percent and is maintained for 12 hours; the illumination intensity is 0lux, the temperature is 20 ℃, and the humidity is 40 percent and is maintained for 12 hours; the 2 protocols were alternated for 30 days. 1 pack was randomly selected from each of 3 groups at 0 day, 7 days, 14 days, 21 days, and 28 days, and verified for virulence attenuation. The selected pathogenic bacteria are aeromonas hydrophila, and the strains are separated from rotten parts of raw fish. The acting concentration of the sample is 1.0ppm, the acting time is 10 minutes, the plate coating count is carried out, the sterilization rate is counted, and the result is shown in Table 3.
TABLE 3
| Numbering | 0 | 7 | 14 | 21 | 28 |
| Example 1 | 100.00％ | 100.00％ | 100.00％ | 100.00％ | 100.00％ |
| Comparative example 2 | 100.00％ | 93.78％ | 88.66％ | 82.56％ | 78.57％ |
| Comparative example 3 | 100.00％ | 97.41％ | 95.88％ | 93.85％ | 90.31％ |
As can be seen from Table 3, the effect of inventive example 1 was not affected by the extreme environment preservation treatment, and 100% inhibition or killing of Aeromonas hydrophila was possible within 4 weeks; comparative example 2 the 30-80 mesh oxone in example 1 was replaced by oxone of more than 100 mesh fineness, the stability of the product was greatly affected, and the sterilization rate was reduced by 21.43% at 4 weeks; in the comparative example 3, the light magnesium carbonate is replaced by the nano zinc oxide, and the sterilization rate is reduced by 9.69% in 4 weeks, which shows that the stability maintenance capability of the nano zinc oxide on the product is superior to that of the light magnesium carbonate.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. The safe disinfectant special for the overwintering culture water body of the raw fish is characterized by comprising the following components in parts by weight: 30-35 parts of potassium hydrogen persulfate composite salt, 25-30 parts of plant astringent, 5-10 parts of sulfamic acid, 20-25 parts of sodium hexametaphosphate, 0.5-1 part of sodium chloride and 1.5-2 parts of nano zinc oxide, wherein the plant astringent is as follows: the mineral source potassium fulvate and the tannin have the mass ratio of (4-5) to 1 in sequence.
2. The safe disinfectant special for the living fish overwintering culture water body as claimed in claim 1, wherein the particle size of the potassium hydrogen persulfate composite salt, the sulfamic acid and the sodium chloride is 30-80 meshes.
3. The safe disinfectant special for the living fish overwintering culture water body as claimed in claim 1, wherein the grain size of the mineral potassium fulvate and the mineral tannic acid is more than 100 meshes.
4. The safe disinfectant special for the living fish overwintering culture water body as claimed in claim 1 or 3, wherein the grain size of the mineral source fulvic acid potassium and the mineral source tannic acid is 100-200 meshes.
5. The preparation method of the safe disinfectant special for the living fish overwintering culture water body as claimed in claim 1, which is characterized by comprising the following steps:
s1, mixing potassium hydrogen persulfate composite salt with nano zinc oxide;
s2, mixing mineral potassium fulvate with the substances in the step S1;
s3, adding sulfamic acid, sodium hexametaphosphate and sodium chloride into the mixture obtained in the step S2;
s4, finally, the tannic acid and the substances in the step S3 are fully and uniformly mixed and packaged.
6. The use of the safe disinfectant special for the living fish overwintering culture water body of claim 1 in the preparation of preparations for preventing and/or treating the rotten body disease of the living fish.
7. The application of claim 6, wherein the amount of the safe type special for the raw fish overwintering culture water body is 0.3-0.5ppm in the prevention process.
8. The application of claim 6, wherein the safe type special for the raw fish overwintering culture water body is used in an amount of 0.6-1.0ppm for 2-3 days in a treatment process.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022255400A1 (en) * | 2021-06-04 | 2022-12-08 | 住友化学株式会社 | Cleaner for indoor water use sites |
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