CN115843832B - Sterilization composition, bactericide and application thereof - Google Patents

Sterilization composition, bactericide and application thereof Download PDF

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CN115843832B
CN115843832B CN202211465038.7A CN202211465038A CN115843832B CN 115843832 B CN115843832 B CN 115843832B CN 202211465038 A CN202211465038 A CN 202211465038A CN 115843832 B CN115843832 B CN 115843832B
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trichoderma
bactericide
agent
acetate
bactericidal composition
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CN115843832A (en
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孙红军
南艳
黄有中
周颖
姜金池
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Jiangsu Dongbao Agrochemical Co ltd
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Abstract

The invention provides a sterilization composition, a bactericide and application thereof, belonging to the technical field of pesticides; the bactericidal composition comprises trichoderma and octylamine acetate; the mass ratio of the trichoderma to the octreotide acetate is (1-8): (1-8); the effective viable count of the trichoderma is more than or equal to 10 hundred million/g. In the invention, trichoderma and octylamine acetate are used in the following steps (1-8): the components (1-8) are mixed in proportion, so that the synergistic effect is obvious, and the growth of the bacterial hyphae of the downy mildew of cucumber can be strongly inhibited. The bactericidal composition adopts the reasonable combination of two pesticides, namely the biological pesticide trichoderma and the low-toxicity high-efficiency pesticide, namely the Xinjunan acetate, can delay the generation of drug resistance of diseases, is simple and safe to use, has good control effect, low residue, is safe to the environment, promotes the safety and high-quality production of crops, and plays an important role in increasing the yield and income of agriculture.

Description

Sterilization composition, bactericide and application thereof
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition and a bactericide and application thereof.
Background
Trichoderma is a class of filamentous, ascomycete fungi, most of which are isolated from a population of soil microorganisms. These fungi are mainly applied in the production in the form of biocontrol bacteria and can promote the growth and development of plants, and inhibit plant diseases in soil and on the ground in a direct or indirect way, but have the difference of action mechanisms. Trichoderma can play a strong role in soil or plant roots, and mainly has strong and rapid reproductive capacity and strong survivability under adverse conditions; meanwhile, the microbial agent also has the capability of efficiently utilizing nutrients, regulating the microbial flora structure of the root and resisting pathogenic bacteria. The trichoderma has the functions of biocontrol and plant growth promotion, mainly prevents and treats soil-borne diseases of various plants, and has a certain prevention and treatment effect on partial ear and leaf diseases. The single trichoderma has higher cost and expense for controlling the main diseases of the main cash crops, and has certain difficulty in market development.
The octyl fungus amine acetate can achieve the sterilization (virus) effect by destroying cell membranes of various pathogens, coagulating proteins, preventing respiration, ferment activity and the like. The medicine has certain systemic and penetrating effects, and has obvious killing and inhibiting effects on various plant fungi, bacteria and viruses which cause crop diseases. The single use of the cinerea acetate has longer time limit for preventing and controlling main diseases of main economic crops, and obviously enhances the drug resistance of the crop diseases.
At present, no report on the synergistic effect of the combination of trichoderma and Xinjunan acetate exists in the prior art.
Disclosure of Invention
The invention aims to provide a sterilizing composition, a bactericide and application thereof.
The invention provides a sterilization composition, which comprises trichoderma and octylamine acetate; the mass ratio of the trichoderma to the octreotide acetate is (1-8): (1-8); the effective viable count of the trichoderma is more than or equal to 10 hundred million/g.
Preferably, the mass ratio of the trichoderma to the octylamine acetate is (1-4): (1-4).
Preferably, the mass ratio of the trichoderma to the octylamine acetate is (1-2): (1-2).
The invention provides a bactericide, which comprises the bactericidal composition, an auxiliary agent and a filler.
Preferably, the mass percentage of the bactericidal composition in the bactericide is 2-70%.
Preferably, the dosage form of the bactericide comprises an aqueous agent or a suspending agent.
Preferably, when the dosage form of the bactericide is an aqueous solution, the auxiliary agent comprises an emulsifier; when the bactericide is in the form of a suspending agent, the auxiliary agent comprises a dispersing wetting agent, an anti-freezing agent, a thickening agent and an antifoaming agent.
Preferably, the emulsifier comprises alkylphenol ethoxylates and/or nonylphenol ethoxylates; the dispersing wetting agent comprises nonylphenol polyoxyethylene ether; the antifreeze agent comprises one or more of propylene glycol, glycerol and ethylene glycol; the thickener comprises xanthan gum and/or magnesium aluminum silicate; the defoamer comprises a silicone defoamer.
Preferably, when the dosage form of the bactericide is a suspending agent, the mass percentage of the dispersing wetting agent in the bactericide is 1-15%; the mass percentage of the antifreeze in the bactericide is 0.3% -3%; the mass percentage of the thickener in the bactericide is 0.4-5%; the mass percentage of the defoamer in the bactericide is 0.3-2%.
The invention also provides the bactericidal composition or the application of the bactericidal agent in preventing and treating plant diseases, wherein the plant diseases comprise cucumber downy mildew or rice bacterial leaf streak.
The invention provides a sterilization composition, which comprises trichoderma and octylamine acetate; the mass ratio of the trichoderma to the octreotide acetate is (1-8): (1-8); the effective viable count of the trichoderma is more than or equal to 10 hundred million/g. In the invention, trichoderma and octylamine acetate are used in the following steps (1-8): (1-8) has obvious synergistic effect, and can strongly inhibit the growth of harmful bacteria, such as cucumber downy mildew hypha. The bactericidal composition adopts the reasonable combination of two pesticides, namely the biological pesticide trichoderma and the low-toxicity high-efficiency pesticide, namely the Xinjunan acetate, can delay the generation of drug resistance of diseases, is simple and safe to use, has good control effect, low residue, is safe to the environment, promotes the safety and high-quality production of crops, and plays an important role in increasing the yield and income of agriculture. The bactericidal composition is used for preventing and treating plant diseases, has good field efficacy for preventing and treating cucumber downy mildew, and has 80% of prevention effect on the cucumber downy mildew. Meanwhile, the bactericidal composition is applied to rice bacterial leaf spot, and the overall control effect is over 80 percent.
Detailed Description
The invention provides a sterilization composition, which comprises trichoderma and octylamine acetate; the mass ratio of the trichoderma to the octreotide acetate is (1-8): (1-8); the effective viable count of the trichoderma is more than or equal to 10 hundred million/g.
In the present invention, the bactericidal composition is preferably composed of trichoderma and octylamine acetate.
In the invention, the mass ratio of the trichoderma to the octreotide acetate is preferably (1-4): (1 to 4), more preferably (1 to 2): (1-2), most preferably 1:2.
In the invention, trichoderma and octylamine acetate are used in the following steps (1-8): (1-8) has obvious synergistic effect, and the synergistic effect of the trichoderma and the Xinjunan mixed in the proportion of 1:2 is most obvious.
In the present invention, the trichoderma is preferably trichoderma asperellum; the trichoderma is not limited to trichoderma of a specific strain, and can be obtained by using commercially available trichoderma. In the implementation process of the invention, the trichoderma is preferably purchased from Libao biotechnology limited company of Heilongjiang, in particular trichoderma asperellum, and the spore content is more than or equal to 10 hundred million/g.
In the invention, the mass of the octreotide acetate is calculated by the pure mass of the octreotide acetate; the octylamine acetate is commercially available from conventional sources. In the specific implementation process of the invention, the octyl fungus amine acetate is preferably purchased from Shandong Shengbang green field chemical Co., ltd, and the mass percentage of the octyl fungus amine acetate in the parent medicine is 40%.
The invention provides a bactericide, which comprises the bactericidal composition, an auxiliary agent and a filler.
In the invention, the bactericide consists of the bactericidal composition, the auxiliary agent and the filler in the scheme.
In the present invention, the mass percentage of the bactericidal composition in the bactericide is preferably 2% to 70%, more preferably 5% to 50%, still more preferably 10% to 20%, and most preferably 15%.
In the invention, the mass percentage of the auxiliary agent in the bactericide is preferably 10-12%, more preferably 11%.
In the present invention, the dosage form of the bactericide preferably includes an aqueous agent or a suspending agent.
In the present invention, when the formulation of the bactericide is an aqueous solution, the auxiliary agent preferably includes an emulsifier; when the bactericide is in the form of a suspension, the auxiliary agent preferably includes a dispersing wetting agent, an anti-freezing agent, a thickener and an antifoaming agent.
In the present invention, the emulsifier preferably includes alkylphenol ethoxylates and/or nonylphenol ethoxylates.
In the present invention, the dispersing wetting agent preferably includes nonylphenol polyoxyethylene ether (NP-9).
In the invention, the antifreeze agent preferably comprises one or more of propylene glycol, glycerol and ethylene glycol.
In the present invention, the thickener preferably comprises xanthan gum and/or magnesium aluminum silicate.
In the present invention, the antifoaming agent preferably includes a silicone antifoaming agent (SAG 612).
In the invention, when the dosage form of the bactericide is a suspending agent, the mass percentage of the dispersing wetting agent in the bactericide is preferably 1-15%, more preferably 5-8%; the mass percentage of the antifreeze in the bactericide is preferably 0.3-3%, more preferably 0.5-1%; the mass percentage of the thickener in the bactericide is preferably 0.4-5%, more preferably 1-3%; the mass percentage of the defoaming agent in the bactericide is preferably 0.3-2%, more preferably 0.5-1%.
In the present invention, the mass percentage of the filler in the bactericide is preferably 73% to 75%, more preferably 74%.
In the present invention, the filler includes water; the water is preferably deionized water.
The invention also provides a preparation method of the bactericide according to the scheme, which comprises the following steps:
mixing trichoderma, octylamine acetate, an auxiliary agent and a filler to obtain the bactericide.
In the present invention, the mixing is preferably stirring mixing; the mixing is preferably carried out in an enamel stirred tank.
In the present invention, when the formulation of the bactericide is a suspending agent, after mixing the trichoderma, the octylamine acetate, the auxiliary agent and the filler, it is preferable to further include grinding the mixed mixture; the grinding is preferably performed by a sand mill; the particle size of the ground product is preferably < 5 mu m, and the particle size can enable the suspension rate of the product to reach more than 98%.
The invention also provides the bactericidal composition or the application of the bactericidal agent in preventing and treating plant diseases, wherein the plant diseases comprise cucumber downy mildew or rice bacterial leaf streak.
In the present invention, the mode of application of the bactericidal composition or the bactericide includes spraying; the application amount of the bactericide is preferably 3000 times of 40-100 mL/mu, more preferably 3000 times of 80-100 mL/mu.
The invention adopts the reasonable combination and the mixed use of chemical pesticides, namely the Xinjunan acetate and the biological pesticide, namely the trichoderma, not only maintains the broad sterilization spectrum of the Xinjunan acetate and the trichoderma and the advantage of good disease control effect, but also overcomes the defects of the Xinjunan acetate and the trichoderma, increases the broad spectrum, the quick action and the lasting effect of the products, realizes the pollution-free control aim of crop diseases, reduces the toxicity to other organisms, reduces the residues of chemical substances in crops, expands the sterilization spectrum and reduces the cost, thereby meeting the requirements of China on the use of low-toxicity efficient pesticides for the crop production of cucumbers, rice and the like.
In order to further illustrate the present invention, a bactericidal composition and a bactericide and applications thereof provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
In order to determine the antibacterial effect of different proportions of trichoderma and Xinjunan acetate on cucumber downy mildew, the indoor toxicity of different proportions of trichoderma and Xinjunan acetate on cucumber downy mildew is measured, and the corresponding concentrations are prepared according to the weight percentages in table 1, wherein the effective viable count of trichoderma is 50 hundred million/gram. The test results are shown in table 1:
table 1 description of results of determination of virulence of trichoderma and Xinjunan acetate and its mixture against cucumber downy mildew
The measurement results in the table 1 show that the trichoderma and the Xinjunan are mixed according to the mass ratio of 8:1-1:8, the growth of cucumber downy mildew hypha can be greatly inhibited, and the synergy of the 1:2 mixing is most obvious.
Example 2
Preparing 10% octyl fungus amine acetate trichoderma aqua.
The raw materials account for the total feeding mass percent:
the effective viable count of trichoderma is 10 hundred million/g.
Weighing the materials according to the weight proportion, sequentially adding the materials under stirring, stirring and uniformly mixing to obtain a product, and performing field efficacy test for controlling cucumber downy mildew, wherein the test process is as follows:
test time 2021, 6 months, place: the process of the Jiangdu area Wu Qiaozhen in Yangzhou city in Jiangsu province: in the test, cucumber is cultivated in open field, and one cucumber variety is adopted. The conditions of each cell are tested to be uniform and consistent. Each treatment was repeated four times for a total of 24 cells, with a random block arrangement of cells. The application period is as follows: the medicine is sprayed for 1 time in the onset period of cucumber downy mildew, and the application dosage is that: 10% of Xinjunan acetate and trichoderma aqueous solution, 80 mL/mu, 60 mL/mu and 40 mL/mu, and a control medicament: 10 hundred million/gram trichoderma aqueous solution: 50 mL/mu; 5% octyl fungus amine acetate aqua: 500 mL/mu, 3000 times of liquid is adopted. Drug effect investigation:
five-point sampling is adopted in each cell, 2-3 plants are investigated at each point, the investigation results are real-time, 3-5 are investigated at each plant, and the investigation results are recorded according to the grading standard. Disease number investigation, immediately prior to administration, investigation time and number of times during administration were determined based on disease progression and requirements, with the last investigation being 10 days after the last administration.
Disease index = 100×Σ (number of leaves at each stage x representative value at each stage)/(total leaves investigated x representative value at highest stage);
disease control effect (%) = [ (control area disease index-control area disease index)/control area disease index ] ×100; or,
disease control effect (%) = [ (control area incidence-control area incidence)/control area incidence ] ×100.
The test results are shown in Table 2.
Table 2:10% of the water aqua of the octylamine and the trichoderma acetate has a 10-day control effect (%)
The results in Table 2 show that the 10% octyl fungus amine trichoderma acetate aqueous solution has good efficacy, has more than 80% control effect on cucumber downy mildew, has remarkable advantages of nuisance-free biological control, and is beneficial to agricultural popularization and application.
Example 3
Preparing 10% of the octylamine acetate trichoderma suspending agent.
The raw materials account for the total feeding mass percent:
the effective viable count of trichoderma is 10 hundred million/g.
The materials are weighed according to the weight proportion, and the products are obtained by sanding through a sand mill after being stirred and mixed uniformly. The dispersing agent adopted in the product formula is nonylphenol polyoxyethylene ether (NP-9), and 8% of the dispersing agent is added into the suspending agent formula, so that the suspension rate of the preparation can reach 99%, and the drug effect of the product is improved by more than 5%.
The product is used for field efficacy test for controlling cucumber downy mildew, and the test process is as follows:
test time 2021, 6 months, place: the process of the Jiangdu area Wu Qiaozhen in Yangzhou city in Jiangsu province: in the test, cucumber is cultivated in open field, and one cucumber variety is adopted. The conditions of each cell are tested to be uniform and consistent. Each treatment was repeated four times for a total of 24 cells, with a random block arrangement of cells. The application period is as follows: the medicine is applied in the onset period of cucumber downy mildew, and the application dosage is as follows: 10% of Xinjunan acetate trichoderma suspending agent, 80 mL/mu, 60 mL/mu and 40 mL/mu, and a control medicament: 10 hundred million/gram trichoderma aqueous solution: 50 mL/mu; 5% octyl fungus amine acetate aqua: 500 mL/mu, 3000 times of liquid is adopted. Drug effect investigation:
five-point sampling is adopted in each cell, 2-3 plants are investigated at each point, the investigation results are real-time, 3-5 are investigated at each plant, and the investigation results are recorded according to the grading standard. Disease number investigation, immediately prior to administration, investigation time and number of times during administration were determined based on disease progression and requirements, with the last investigation being 10 days after the last administration. The test results are shown in Table 3.
Table 3:10% Xinjunan acetate trichoderma suspending agent and 10 days after preventing and controlling cucumber downy mildew
The results shown in Table 3 show that the 10% octyl fungus amine acetate trichoderma suspending agent has the control effect on cucumber downy mildew of more than 80%, has the remarkable advantages of nuisance-free biological control, and is beneficial to agricultural popularization and application.
Meanwhile, the product is used for field efficacy tests for preventing and treating rice bacterial leaf spot, and the test process is as follows:
test time 2021, 6 months, place: the Yangzhou city Jiangdu area in Jiangsu province is suitable for the tomb town, and the process is as follows: the test adopts the cluster village to transplant the paddy rice field. Dividing test cells, and uniformly testing conditions of each cell. Each treatment was repeated four times for a total of 24 cells, with a random block arrangement of cells. The application period is as follows: the application of the medicine is carried out in the onset period of the bacterial leaf spot of rice, and the application dosage is that: 10% of Xinjunan acetate trichoderma suspending agent 100 mL/mu, 80 mL/mu and 60 mL/mu, and a control medicament: 10 hundred million/gram trichoderma aqueous solution: 50 mL/mu; 5% octyl fungus amine acetate aqua: 200 mL/mu, 3000 times of liquid is adopted.
Drug effect investigation:
five-point sampling is adopted in each cell, 2-3 plants are investigated at each point, the investigation results are real-time, 3-5 are investigated at each plant, and the investigation results are recorded according to the grading standard. Disease number investigation, immediately prior to administration, investigation time and number of times during administration were determined based on disease progression and requirements, with the last investigation being 10 days after the last administration.
Table 410% Xinjunan acetate trichoderma suspending agent for preventing and treating rice bacterial leaf spot disease 10 days after the drug
As can be seen from Table 4, the 10% octopamine acetate trichoderma suspending agent has the prevention effect on rice bacterial leaf streak more than 80%, has the remarkable advantages of nuisance-free biological prevention and control, and is beneficial to agricultural popularization and application.
Comparative example 1
The same procedure as in example 3 was repeated except for using nonylphenol polyoxyethylene ether (NP-10) instead of nonylphenol polyoxyethylene ether (NP-9) and nonylphenol polyoxyethylene ether (NP-10) 8%.
The test results show that: the control efficiency on the bacterial leaf spot of rice is 80.62 percent, 78.36 percent and 75.06 percent for 60 mL/mu. Therefore, the 10% octyl fungus amine acetate trichoderma suspending agent prepared by adopting the nonylphenol polyoxyethylene ether (NP-9) has better control effect than the 10% octyl fungus amine acetate trichoderma suspending agent prepared by adopting the nonylphenol polyoxyethylene ether (NP-10).
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.

Claims (10)

1. A bactericidal composition characterized by comprising trichoderma and octylamine acetate; the mass ratio of the trichoderma to the octreotide acetate is (1-8): (1-8); the effective viable count of the trichoderma is more than or equal to 10 hundred million/g; the trichoderma is trichoderma asperellum.
2. The bactericidal composition of claim 1, wherein the mass ratio of trichoderma to octreotide acetate is (1-4): (1-4).
3. The bactericidal composition of claim 1, wherein the mass ratio of trichoderma to octylamine acetate is (1-2): (1-2).
4. A bactericide comprising the bactericidal composition according to any one of claims 1 to 3, an auxiliary agent and a filler.
5. The bactericide according to claim 4, wherein the mass percentage of the bactericidal composition in the bactericide is 2-70%.
6. A biocide as claimed in claim 4 or claim 5 wherein the biocide is in a dosage form comprising an aqueous or suspension agent.
7. The bactericide according to claim 6, characterized in that when the dosage form of the bactericide is an aqueous agent, the auxiliary agent comprises an emulsifier;
when the bactericide is in the form of a suspending agent, the auxiliary agent comprises a dispersing wetting agent, an anti-freezing agent, a thickening agent and an antifoaming agent.
8. The bactericide according to claim 7, characterized in that the emulsifier comprises alkylphenol ethoxylates and/or nonylphenol ethoxylates;
the dispersing wetting agent comprises nonylphenol polyoxyethylene ether; the antifreeze agent comprises one or more of propylene glycol, glycerol and ethylene glycol; the thickener comprises xanthan gum and/or magnesium aluminum silicate; the defoamer comprises a silicone defoamer.
9. The bactericide according to claim 7 or 8, wherein when the bactericide is in the form of a suspension, the mass percentage of the dispersing wetting agent in the bactericide is 1% -15%; the mass percentage of the antifreeze in the bactericide is 0.3% -3%; the mass percentage of the thickener in the bactericide is 0.4-5%; the mass percentage of the defoamer in the bactericide is 0.3-2%.
10. Use of the fungicidal composition of any one of claims 1 to 3 or the fungicidal agent of any one of claims 4 to 9 for controlling plant diseases including cucumber downy mildew or bacterial leaf streak of rice.
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Citations (3)

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Publication number Priority date Publication date Assignee Title
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CN105961379A (en) * 2016-05-04 2016-09-28 南京高正农用化工有限公司 Large composition floating granule for preventing and controlling sheath blight and rice blast of rice, and preparation method thereof
CN111410578A (en) * 2020-03-25 2020-07-14 云南恒卓农业科技有限公司 Pesticide bacterial fertilizer for preventing and treating diseases and pests of potatoes and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN105076218A (en) * 2015-08-26 2015-11-25 山东中新科农生物科技有限公司 Sterilizing composition
CN105961379A (en) * 2016-05-04 2016-09-28 南京高正农用化工有限公司 Large composition floating granule for preventing and controlling sheath blight and rice blast of rice, and preparation method thereof
CN111410578A (en) * 2020-03-25 2020-07-14 云南恒卓农业科技有限公司 Pesticide bacterial fertilizer for preventing and treating diseases and pests of potatoes and preparation method thereof

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