CN117481123B - Agricultural bactericidal composition - Google Patents
Agricultural bactericidal composition Download PDFInfo
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- CN117481123B CN117481123B CN202311851512.4A CN202311851512A CN117481123B CN 117481123 B CN117481123 B CN 117481123B CN 202311851512 A CN202311851512 A CN 202311851512A CN 117481123 B CN117481123 B CN 117481123B
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- kasugamycin
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- propiconazole
- bactericidal composition
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 55
- 239000000203 mixture Substances 0.000 title claims abstract description 51
- PVTHJAPFENJVNC-MHRBZPPQSA-N kasugamycin Chemical compound N[C@H]1C[C@H](NC(=N)C(O)=O)[C@@H](C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H]1O PVTHJAPFENJVNC-MHRBZPPQSA-N 0.000 claims abstract description 64
- 241000209094 Oryza Species 0.000 claims abstract description 59
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 58
- 235000009566 rice Nutrition 0.000 claims abstract description 58
- 239000005822 Propiconazole Substances 0.000 claims abstract description 38
- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000230 xanthan gum Substances 0.000 claims abstract description 24
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 24
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 24
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 24
- 208000031888 Mycoses Diseases 0.000 claims abstract description 7
- 239000004480 active ingredient Substances 0.000 claims abstract description 4
- 241001530056 Athelia rolfsii Species 0.000 claims description 6
- 230000000855 fungicidal effect Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 70
- 230000005764 inhibitory process Effects 0.000 abstract description 23
- 230000002265 prevention Effects 0.000 abstract description 15
- 230000001954 sterilising effect Effects 0.000 abstract description 9
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 8
- 238000011282 treatment Methods 0.000 abstract description 6
- 241001330975 Magnaporthe oryzae Species 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 24
- 201000010099 disease Diseases 0.000 description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 21
- 239000003899 bactericide agent Substances 0.000 description 20
- 230000002195 synergetic effect Effects 0.000 description 9
- -1 sienna vitamin Chemical class 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000000375 suspending agent Substances 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 description 5
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 5
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000004530 micro-emulsion Substances 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 2
- 239000006229 carbon black Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 229940051841 polyoxyethylene ether Drugs 0.000 description 2
- 229920000056 polyoxyethylene ether Polymers 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241001220430 Aphelenchus Species 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 241001474928 Ustilaginoidea virens Species 0.000 description 1
- 241000529915 Xylophilus Species 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 230000005584 early death Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- UFHLMYOGRXOCSL-UHFFFAOYSA-N isoprothiolane Chemical compound CC(C)OC(=O)C(C(=O)OC(C)C)=C1SCCS1 UFHLMYOGRXOCSL-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 238000002723 toxicity assay Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an agricultural sterilization composition, and belongs to the technical field of agricultural compositions. The bactericidal composition comprises active ingredients of kasugamycin and propiconazole, and also comprises xanthan gum, wherein the mass ratio of the propiconazole to the kasugamycin is 0.5-1.0:5-8, and the addition amount of the xanthan gum is 0.3% by mass percent. The bactericidal composition provided by the invention has a prevention and treatment effect on rice fungal diseases and can also improve rice yield. The bactericidal composition has an inhibition effect on the growth of rice blast fungus hypha, the inhibition effect is 85.80-93.91%, the control effect on rice sheath blight is 81.29-85.47%, and the control effect on false smut is 79.14-87.68%. The invention provides an effective means for preventing and controlling fungal diseases of rice and improving the yield of rice.
Description
Technical Field
The invention belongs to the technical field of agricultural compositions, and relates to an agricultural sterilization composition.
Background
Rice is one of important grain crops, and compared with other crops, water is needed in nearly every growing period of rice, and rice is planted in subtropical and tropical areas in a plurality of ways, so that the rice field environment has the characteristics of warmth and humidity, and is easy to be infected by pathogenic microorganisms. According to incomplete statistics, more than 10 rice diseases caused by infection are most commonly counted, wherein the rice diseases comprise rice blast, banded sclerotial blight, false smut, leaf spot of flax, black powder of rice grain, leaf sheath rot, bacterial leaf blight, bacterial leaf spot, spike blight, stripe leaf blight, aphelenchus xylophilus and the like, and the rice blast, banded sclerotial blight and false smut cause huge losses, and are three important diseases on rice.
At present, the means for preventing and controlling rice diseases mainly comprise biological diversity prevention and control, rice field management, cultivation of resistant varieties and chemical prevention and control, wherein the chemical prevention and control is the most economical and effective means, but the novel variety of the existing bactericide is slow to innovate, the bactericide has the problems of insufficient broad spectrum, poor sterilization effect, phytotoxicity and more soil residues, meanwhile, pathogenic bacteria generate drug resistance due to long-term single application habit, the dosage of the bactericide is increased, the production cost is increased, and more environmental problems are brought. The rice blast bacteria have high genetic diversity, obvious differentiation of the species, easy generation of drug resistance, and low level resistance of the rice blast bacteria to the isoprothiolane. Therefore, the compound bactericidal composition with wide bactericidal spectrum is provided with important significance.
Disclosure of Invention
The invention provides an agricultural bactericidal composition for solving the problems of insufficient broad spectrum and drug resistance caused by long-term single bactericide use of the existing bactericide. The bactericidal composition contains the effective components of the propiconazole and the kasugamycin, has good control effect on rice blast, banded sclerotial blight and false smut, and can also improve the yield of rice. The invention provides an effective means for preventing and controlling fungal diseases of rice and improving the yield of rice.
In order to achieve the technical purpose of the invention, on the one hand, the invention provides a sterilization composition, wherein the effective components of the sterilization composition comprise the benzopropiconazole and the kasugamycin, and the mass ratio of the benzopropiconazole to the kasugamycin is 0.5-1.0:5-8. The bactericidal composition also comprises xanthan gum, and the addition amount of the xanthan gum is 0.3 percent by mass percent.
Furthermore, in the bactericidal composition provided by the invention, the mass ratio of the benzopropiconazole to the kasugamycin is 0.7-1.0:5-7. Preferably, the mass ratio of the benzopropiconazole to the kasugamycin is 0.8:6.
Further, according to the indoor toxicity measurement of the bactericidal composition, the kasugamycin or the propiconazole has an inhibition effect on the growth of pyriform hyphae when being singly used, but the inhibition effect is poor, the inhibition effect is 85.80-93.91% after the kasugamycin and the propiconazole are compounded and used, and the improvement effect is obvious. The kasugamycin and the propiconazole in the bactericidal composition provided by the invention have a synergistic effect, and when the mass ratio of the kasugamycin to the propiconazole is 5-7:0.7-10, the difference between the theoretical inhibition effect and the actual inhibition effect is larger than 8, and the synergistic effect is better.
Further, according to field experiments of the sterilization composition, the single application of the kasugamycin or the propiconazole has weaker prevention effect on rice sheath blight and rice false smut, the prevention effect of the bactericide obtained by compounding the kasugamycin and the propiconazole on the rice sheath blight is 81.29-85.47%, the prevention effect of the bactericide on the rice false smut is 79.14-87.68%, and the prevention effect is greatly improved. The xanthan gum in the agricultural bactericidal composition provided by the invention can improve the bactericidal effect of the bactericide.
In another aspect, the invention claims the use of the above fungicidal composition for the control of fungal diseases of rice, including rice blast, banded sclerotial blight and false smut.
Also, the use of the above-described fungicidal compositions for improving the yield of crops, including rice, is claimed.
Compared with the prior art, the technical scheme provided by the invention has at least the following beneficial effects or advantages:
the agricultural sterilization composition containing kasugamycin and propiconazole provided by the invention has a good prevention and treatment effect on rice fungal diseases, and can also improve the yield of rice, and the effect of the two components in combination is better than that of a single dose, and the composition has a synergistic effect.
According to the indoor toxicity test of the bactericidal composition, the kasugamycin or the propiconazole has an inhibition effect on the growth of pyriform hyphae when being singly used, but the inhibition effect is poor, the inhibition effect is 85.80-93.91% after the kasugamycin and the propiconazole are compounded and used, and the improvement effect is obvious. The bactericide provided by the invention has a synergistic effect, and when the mass ratio of kasugamycin to propiconazole is 5-7:0.7-10, the difference between the theoretical inhibition effect and the actual inhibition effect is larger than 8, and the synergistic effect is better.
According to field experiments of the bactericidal composition, the single application of the kasugamycin or the propiconazole has weaker prevention effect on rice sheath blight and rice false smut, the obtained bactericidal agent after the kasugamycin and the propiconazole are compounded has 81.29-85.47% of prevention effect on rice sheath blight, 79.14-87.68% of prevention effect on rice false smut, and the prevention effect is greatly improved. And when the mass ratio of kasugamycin to propiconazole is 5-7:0.7-10, the control effect on rice sheath blight is more than 83%, the control effect on rice false smut is more than 82%, and the control effect is good. The xanthan gum in the agricultural bactericidal composition provided by the invention can improve the bactericidal effect of the bactericide, and the bactericidal effect is better than that of a common thickener; the xanthan gum in the agricultural bactericidal composition provided by the invention can also improve the rice yield, and the improvement effect is better than that of carboxymethyl cellulose or polyvinyl alcohol.
Detailed Description
The following describes the technical aspects of the present invention with reference to examples, but the present invention is not limited to the following examples. The experimental methods and the detection methods in each embodiment are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.
The suspension of the benzopropiconazole is purchased from Henan Baolan biotechnology Co., ltd, and the content of the original medicine is 30%.
Kasugamycin is purchased from the wuhank biomedical technology limited company, and the content of the original medicine is 70%.
Xanthan gum was purchased from sienna vitamin biotechnology limited and has an effective content of 99%.
Example 1
The present example provides formulation formulations of a bactericidal composition of the combination of benzopropiconazole and kasugamycin.
1. Suspending agent
The suspending agent comprises 8.57 parts of kasugamycin, 2.67 parts of the benzoyl propiconazole, 0.3 part of xanthan gum, 5 parts of calcium dodecyl benzene sulfonate, 3 parts of alkyl naphthalene sulfonate, 0.5 part of glycerol, 0.8 part of white carbon black and the balance of water per 100 parts of suspending agent containing the benzoyl propiconazole and the kasugamycin by mass.
Weighing the components according to the parts by mass, adding the kasugamycin, the propiconazole, the xanthan gum, the calcium dodecyl benzene sulfonate, the alkyl naphthalene sulfonate and the white carbon black into a grinding kettle, grinding to a certain fineness, transferring the materials into the stirring kettle, adding the rest of the glycerol and the water, and fully and uniformly mixing to obtain the suspending agent containing the kasugamycin and the benzoyl propiconazole, wherein the suspending agent contains 6.8 percent.
2. Microemulsion preparation
The microemulsion comprises 11.43 parts of kasugamycin, 1.67 parts of the benzoyl propiconazole, 0.3 part of xanthan gum, 5 parts of isopropanol, 10 parts of tween-80, 5 parts of styrylphenol polyoxyethylene ether, 8 parts of n-butanol and the balance of water in each 100 parts of microemulsion containing the benzoyl propiconazole and the kasugamycin by mass.
Weighing the components according to the parts by mass, dissolving kasugamycin and propiconazole in isopropanol and n-butanol, uniformly mixing with tween-80 and styrylphenol polyoxyethylene ether, preparing the xanthan gum into an aqueous solution, pouring the aqueous solution into the mixed solution, adding residual water, and fully stirring to obtain transparent liquid.
Example 2
This example provides an indoor toxicity assay for a bactericidal composition.
The preparation type used in this example is suspending agent, the preparation method refers to example 1, the suspending agent is used after being diluted 1000 times, and the mass ratio of the effective components is as follows.
The test involved 13 treatments in total, test 1: the mass ratio of the kasugamycin to the propiconazole is 5:1; test 2: the mass ratio of the kasugamycin to the propiconazole is 6:0.8; test 3: the mass ratio of the kasugamycin to the propiconazole is 7:0.7; test 4: the mass ratio of the kasugamycin to the propiconazole is 8:0.5; test 5-8: only kasugamycin is added; test 9-12: adding only the benzopropiconazole; test 13: no bactericide was added and the control group was blank.
The toxicity of the bactericides to Pyricularia oryzae, a bacterial strain of Pyricularia oryzae (purchased from Shanghai Valley research Co., ltd.) was measured by hypha growth inhibition method, and the synergy of the bactericides was measured by growth method three times per group. The pyriform bacteria propagated on PDA medium was beaten into a bacterial cake by a punch with diameter of 0.65cm, inoculated into a mixed culture dish (R=9 cm) containing PDA medium (9 mL) and bactericide (1 mL) by a sterile operation method, the inoculated culture dish was placed in a constant temperature incubator with temperature of 26+ -0.5 ℃ for cultivation, the diameters were taken at three different directions of the bacterial colony and the average value was taken, and the bacteriostasis rate was calculated, and the calculation results are shown in Table 1.
C exp =C 1 +C 2 (1-C 1 )
In the formula, C exp The compound agent has theoretical inhibition effect on germs; c (C) 1 And C 2 Is the actual level of inhibition of pathogens when used alone in a single dose. If the actual inhibition level of the pathogen by the mixture is determined in the test (C obs ) And C exp Equal, each component in the mixture shows independent action; if C obs Greater or less than C exp Indicating that the components in the mixture exhibit synergistic or antagonistic effects.
Table 1: inhibition of pyriform hypha growth by bactericidal composition containing kasugamycin (A) and propiconazole (B)
As shown in Table 1, the inhibition effect of kasugamycin on pyriform hypha growth is 62.13-70.89%, the inhibition effect of the pyriform hypha growth is 47.19-58.61%, and the inhibition effect of the kasugamycin and the pyriform is 85.80-93.91% after the kasugamycin and the pyriform are compounded, so that the improvement effect is obvious. The bactericide provided by the invention has a synergistic effect, when the mass ratio of kasugamycin to propiconazole is 5-7:0.7-10, the difference between the theoretical inhibition effect and the actual inhibition effect is larger than 8, the synergistic effect is better, when the mass ratio of kasugamycin to propiconazole is 6:0.8, the difference between the theoretical inhibition effect and the actual inhibition effect is 9.65, and the synergistic effect is optimal.
Example 3
This example provides a field test of the effect of a fungicidal composition on rice.
The preparation type used in the embodiment is microemulsion, the preparation method refers to the embodiment 1, the spraying amount of each cell is 6kg after 100 times dilution, the preparation is respectively applied to the rice leaf pillow at ordinary times and the ear alignment period, and the mass ratio of the active ingredients is as follows.
The test involved a total of 16 treatments, test 14: the mass ratio of the kasugamycin to the propiconazole is 5:1; test 15: the mass ratio of the kasugamycin to the propiconazole is 6:0.8; test 16: the mass ratio of the kasugamycin to the propiconazole is 7:0.7; test 17: the mass ratio of the kasugamycin to the propiconazole is 8:0.5; test 18-21: only kasugamycin is added; test 22-25: adding only the benzopropiconazole; test 26: no bactericide is added, and the blank control group is adopted; test 27: no xanthan gum is added; test 28: equivalent replacement of xanthan gum with carboxymethyl cellulose; test 29: the xanthan gum was replaced with an equal amount of polyvinyl alcohol.
The test site is Zhejiang Jin Huashui rice planting base, the rice variety to be tested is Jinzao 47, the rice field fertilizer and water management is good, a cell test method is adopted, and the test cell area is 40m 2 Cells are separated by ditches, the random block is arranged, and the method is repeated three times. Transplanting rice seedlings in the middle and late ten days of 4 months, wherein the transplanting density is 16.5 cm multiplied by 20 cm, and each cluster is 4-6 per cluster.
Banded sclerotial blight investigation: each cell was investigated 15d before harvesting, each cell was sampled by random 5-point sampling, each spot was examined for 20 clusters, total number of plants, number of sick plants and disease level were recorded for calculating disease index and control efficiency, and statistical results are shown in table 2. The classification standard of the rice sheath blight disease is as follows: grade 0, no disease of whole plant; stage I, leaf 4 and leaf sheaths and leaf attacks below (sword leaf is taken as leaf 1); stage III, leaf 3 and leaf sheaths below the leaf, leaf onset; stage V, leaf 2 and leaf sheaths below the leaf, leaf onset; stage VII, leaf 1 and leaf sheaths and leaf diseases below the leaf; grade IX, onset and early death of whole plants.
Control effect (%) = [1- (CK) 0 ×PT 1 )/(CK 1 ×PT 0 )]×100
In the formula, CK 0 Is the index of the disease state before the drug administration of the control group; CK (CK) 1 Is the disease index after the control group is dosed; PT (PT) 0 Pre-drug condition index for drug treatment; PT (PT) 1 The disease index is the disease index after the drug treatment.
Investigation of false smut: each cell was investigated 15d before harvesting, a random 5-point sampling method was used for each cell, 20 clusters were searched for each cell, 100 clusters were recorded for total number of plants and number of disease plants, a random 5-point sampling method was used for each cell, 4 clusters were searched for each cell, total number of grains and number of disease grains were recorded for each cell, and the statistical results are shown in table 3.
Yield investigation: the mature period adopts a random 5-point sampling method, 4 samples are taken at each point, the overground part of the rice plant is taken at the interval of the forward running, the yield composition condition is inspected, the yield of each cell is calculated, and the statistical result is shown in table 2.
Table 2: effect of sterilizing composition containing kasugamycin (A) and propiconazole (B) on preventing and controlling Rice sheath blight disease and yield
As shown in Table 2, the single application of the kasugamycin has a control effect on rice sheath blight disease of 35.31-45.77%, the single application of the benzopropiconazole has a control effect on rice sheath blight disease of 50.22-61.34%, the obtained bactericide after the combination of the kasugamycin and the benzopropiconazole has a control effect on rice sheath blight disease of 81.29-85.47%, and the control effect is greatly improved, so that the agricultural bactericidal composition of the kasugamycin and the benzopropiconazole can better improve the control effect on rice sheath blight disease, and the control effect of the kasugamycin and the benzopropiconazole is better when the mass ratio of the kasugamycin to the benzopropiconazole is 5-7:0.7-10, and the control effect is better when the mass ratio of the kasugamycin to the benzopropiconazole is 6:0.8, and the control effect is optimally 85.47%. The agricultural bactericidal composition provided by the invention can also improve the yield of rice, and the improvement effect is 6.81-8.94%. Tests 27-29 show that the xanthan gum in the agricultural bactericidal composition provided by the invention can improve the bactericidal effect of the bactericide and the rice yield, and the prevention effect of the xanthan gum on rice sheath blight disease after being replaced by the same amount of carboxymethyl cellulose or polyvinyl alcohol has a lifting effect compared with that of single-dose application, but the lifting effect is worse than that of the xanthan gum, and the lifting effect of the carboxymethyl cellulose or the polyvinyl alcohol on the rice yield is lower than that of the xanthan gum.
Table 3: bactericidal composition containing kasugamycin (A) and propiconazole (B) has effect of preventing false smut
As shown in Table 3, the single application of kasugamycin has a control effect of 23.01-34.91% on false smut, the single application of the benzopropiconazole has a control effect of 60.28-69.45% on false smut, the compounded kasugamycin and the benzopropiconazole have a control effect of 79.14-87.68% on false smut, and the control effect is greatly improved, which indicates that the agricultural sterilization composition of the kasugamycin and the benzopropiconazole can better improve the control effect of paddy on false smut, and the control effect of the kasugamycin and the benzopropiconazole is better when the mass ratio of the kasugamycin to the benzopropiconazole is 5-7:0.7-10, and the control effect of the kasugamycin and the benzopropiconazole is better when the mass ratio of the kasugamycin to the benzopropiconazole is 6:0.8, and the control effect is optimal to be 87.68%. Experiments 27-29 show that the xanthan gum in the agricultural bactericidal composition provided by the invention can improve the bactericidal effect of the bactericide, has a better improving effect on the prevention effect of false smut of rice after the xanthan gum is replaced by equivalent carboxymethyl cellulose or polyvinyl alcohol compared with single-dose application, and has poorer improving effect than the xanthan gum.
The embodiments described above are some, but not all, embodiments of the invention. The detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments obtained without inventive effort by a person skilled in the art, which are related deductions and substitutions made by the person skilled in the art under the condition of the inventive concept, are within the scope of protection of the present invention.
Claims (6)
1. The bactericidal composition is characterized in that the active ingredients comprise kasugamycin and propiconazole;
the active ingredients of the bactericidal composition also comprise xanthan gum;
the mass ratio of the benzopropiconazole to the kasugamycin is 0.5-1.0:5-8;
the addition amount of the xanthan gum is 0.3 percent by mass percent.
2. The bactericidal composition according to claim 1, wherein the mass ratio of the propiconazole to the kasugamycin is 0.7-1.0:5-7.
3. The bactericidal composition of claim 2, wherein the mass ratio of benzopropiconazole to kasugamycin is 0.8:6.
4. Use of the bactericidal composition as set forth in any one of claims 1 to 3 for controlling fungal diseases of rice.
5. The use according to claim 4, wherein the fungal diseases include rice blast, banded sclerotial blight and false smut.
6. Use of the fungicidal composition according to any one of claims 1-3 for increasing the yield of crops, including rice.
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