CN112891280A

CN112891280A - Composition containing wolfberry stem cells and capable of improving skin firmness and application of composition

Info

Publication number: CN112891280A
Application number: CN202110386538.0A
Authority: CN
Inventors: 张应贤; 李湘
Original assignee: Guangzhou Lakel Stem Cell Research Institute
Current assignee: Guangzhou Lakel Stem Cell Research Institute
Priority date: 2021-04-12
Filing date: 2021-04-12
Publication date: 2021-06-04

Abstract

The invention discloses a composition containing wolfberry stem cells and capable of improving skin firmness, which comprises the following components in parts by weight: 0.1-0.5 part of medlar extract, 2-10 parts of lactobacillus fermentation product, 0.1-0.8 part of nano biological firming agent, 0.05-0.2 part of acetylated sodium hyaluronate, 0.1-0.5 part of phenoxyethanol, 0.8-2 parts of 1,2 pentanediol, 0.5-2 parts of glycerol glucoside, 8-18 parts of schizosaccharomyces cerevisiae fermentation filtrate, 0.2-0.6 part of tocopherol acetate, 0.1-0.5 part of hydrogenated lecithin and 0.5-1 part of squalane. Compared with the prior art, the invention has the advantages that: the composition with the effect of improving skin firmness can activate muscle bottom cells and accelerate skin metabolism; supplementing moisture to skin, increasing moisture retention of skin, and improving skin firmness.

Description

Composition containing wolfberry stem cells and capable of improving skin firmness and application of composition

Technical Field

The invention relates to the technical field of cosmetics, in particular to a composition containing wolfberry stem cells and capable of improving skin firmness and application thereof.

Background

The first sign of aging is not wrinkles, but laxity. The facial tightening and lifting are a key step for keeping the facial contour young, and with the increase of age, collagen and elastic fiber protein in the dermis of the skin gradually lose and decrease, the supporting force of the skin is reduced, the elasticity is reduced, the skin loses elasticity and starts to droop from the canthus and the mouth corner, and the contour of the whole face begins to deform afterwards.

The sagging of the skin is hard to observe from the front because women always involuntarily show the most beautiful expression when looking at the mirror. The center of the face is the part where the skin is first perceived to be loose, and the parts from the inner canthus to the two sides of the nose are recessed and are the characteristic of the beginning of the skin loosening. The contour of the face also changes gradually, but is not easily perceived at an early stage. Originally, the round face or the low nose bridge, when a person surprises to find that the face contour becomes clear in a certain day, the fact is true that the skin begins to relax.

The upper eyelid skin begins to loosen first and sometimes a person with a single eyelid may become double-edged. The skin of the lower eyelid then also becomes loose, and wrinkles appear concentrically with the eyelid. The shadow of the corner edge of the eye is also apparent. In addition, the sagging corners of the mouth appear as shadows between the mandible. In this case, the skin is more flabby. With age, the elastic fibers of the skin decrease causing the skin to sag and eventually the overall facial contour becomes uneven. The face was not fat, but significant double chin appeared.

Disclosure of Invention

The invention aims to provide a composition with the effect of improving skin firmness, which can activate muscle bottom cells and accelerate skin metabolism; the composition containing the medlar stem cells and capable of improving the skin firmness can replenish moisture to the skin, increase the moisture retention of the skin and improve the skin firmness, and the application thereof.

The purpose of the invention is realized by the following technical scheme:

the composition containing the medlar stem cells and capable of improving skin firmness comprises the following components in parts by weight: 0.1-0.5 part of medlar extract, 2-10 parts of lactobacillus fermentation product, 0.1-0.8 part of nano biological firming agent, 0.05-0.2 part of acetylated sodium hyaluronate, 0.1-0.5 part of phenoxyethanol, 0.8-2 parts of 1,2 pentanediol, 0.5-2 parts of glycerol glucoside, 8-18 parts of schizosaccharomyces cerevisiae fermentation filtrate, 0.2-0.6 part of tocopherol acetate, 0.1-0.5 part of hydrogenated lecithin and 0.5-1 part of squalane.

Further, the composition comprises the following components in parts by weight: 0.28 part of medlar extract, 6.5 parts of lactobacillus fermentation product, 0.6 part of nano biological firming agent, 0.12 part of acetylated sodium hyaluronate, 0.35 part of phenoxyethanol, 1.5 parts of 1,2 pentanediol, 1.5 parts of glycerol glucoside, 12 parts of yeast fermentation filtrate, 0.35 part of tocopheryl acetate, 0.5 part of hydrogenated lecithin and 0.8 part of squalane.

Further, the composition comprises the following components in parts by weight: 0.1 part of medlar extract, 2 parts of lactobacillus fermentation product, 0.1 part of nano biological firming agent, 0.05 part of acetylated sodium hyaluronate, 0.1 part of phenoxyethanol, 0.8 part of 1,2 pentanediol, 0.5 part of glycerol glucoside, 8 parts of yeast schizophyllum fermentation filtrate, 0.2 part of tocopheryl acetate, 0.1 part of hydrogenated lecithin and 0.5 part of squalane.

Further, the composition comprises the following components in parts by weight: 0.5 part of medlar extract, 10 parts of lactobacillus fermentation product, 0.8 part of nano biological firming agent, 0.2 part of acetylated sodium hyaluronate, 0.5 part of phenoxyethanol, 2 parts of 1,2 pentanediol, 2 parts of glycerol glucoside, 18 parts of yeast fermentation filtrate, 0.6 part of tocopherol acetate, 0.5 part of hydrogenated lecithin and 1 part of squalane.

Further, the medlar extract is a medlar stem cell culture extract.

The application of the composition containing the medlar stem cells and capable of improving the skin firmness comprises the following steps:

1) accurately weighing fructus Lycii extract, lactobacillus fermentation product, nanometer biological firming agent, acetylated sodium hyaluronate, phenoxyethanol, 1,2 pentanediol, and glycerol dextran, stirring at 43 deg.C at 500r/min, mixing, and dissolving to obtain phase A;

2) coating phase A with tocopheryl acetate, hydrogenated lecithin and squalane to obtain microcapsule inclusion;

3) accurately weighing xanthan gum, sodium polyacryloyl dimethyl taurate, glycerol, 4 parts of propylene glycol, carboxymethyl chitosan, a preservative PEHG and deionized water, stirring at a constant temperature of 43 ℃ and a speed of 500r/min, and dissolving uniformly to prepare a phase B;

4) stirring the inclusion at a constant temperature of 43 ℃ and adding the inclusion into the phase B, stirring at a speed of 500r/min for 10min, and standing to recover the room temperature.

Compared with the prior art, the invention has the advantages that:

the fructus Lycii extract is prepared by extracting stem cells of fructus Lycii plant from fructus Lycii seedling as stem cell culture, and can activate skin stem cells.

A lactobacillus fermented product is prepared by naturally fermenting natural Chinese herbal medicines (folium Artemisiae Argyi, flos Lonicerae, and herba Hedyotidis Diffusae) with precious plant tender bud to obtain lactobacillus flora fermented liquid. After several weeks of slow fermentation in anaerobic environment, the carbohydrate and sugar are converted into skin-friendly protein, vitamins and amino acids, the effective components are decomposed and micronized, and harmful bacteria and toxins are naturally eliminated, thus being natural and safe. Meanwhile, effective components are changed into micromolecules which are easier to absorb through fermentation, so that the activity effect is improved, and the permeability is better. The botanical lactobacillus contains rich enzymes, promotes the metabolism of cells, enhances the synthesis of collagen, improves the self-repairing capacity of skin, enhances the skin barrier, removes cell free radicals, inhibits lipid peroxidation, activates the organism repairing performance, has excellent smooth and tender skin effect, has stronger functions of moistening, repairing and relieving, helps the skin to recover natural luster, reduces fine wrinkles, improves the density and concentration of skin nutrients such as peptide, protein, antioxidant and amino acid, multiplies the efficacy of the product with the same dosage, and has super efficacy of the fermented skin care product, so that the formula is more effective, safer and more environment-friendly.

The nanometer biological firming agent comprises water, hyaluronic acid, acacia gum extract, phenoxyethanol and caprylyl glycol, wherein the acacia is coated with micromolecular hyaluronic acid by a coating technology to enable the particle size to reach about 200nm, and the acacia is deeply permeated, plump and filled in the nanometer biological firming agent and has strong hydration effect; promoting collagen synthesis, stimulating fibroblast proliferation, and improving skin compactness.

Acetylated sodium hyaluronate (AcHA), obtained by acetylating and substituting hydroxyl in HA molecular structure with acetyl, is also called sodium hyaluronate double, and HAs a moisturizing effect 2 times that of common HA. The introduction of acetyl groups enables AcHA to have both hydrophilicity and lipophilicity, have higher water retention and biological activity, and be firmly maintained on the surface of the skin to prevent water from evaporating from the skin; but also can permeate epidermis to hydrate and soften the horny layer, and the inner part and the outer part simultaneously act, thereby playing double moisturizing effects, enhancing the barrier of the horny layer, improving the dry and rough state of the skin and enabling the skin to be soft and elastic.

The secondary fission yeast fermentation product filtrate is an active substance extracted from a lysate of bifidobacterium which is a probiotic by applying biotechnology, can effectively protect the skin from being damaged by ultraviolet rays and supports an endogenous protection mechanism of the skin; the cell repair is promoted, the cell regeneration is promoted and the resistance is enhanced by effectively activating the anti-immunosuppression process at the cellular level; accelerating the skin regeneration speed, and playing the efficacies of repairing, moisturizing and enhancing the skin elasticity.

Glycerol glucoside: the dead grass during blooming is extracted and refined, and the skin cell activity and metabolism are enhanced; restoring aged cells to a young cell state; promoting the generation of collagen, slowing down the aging process of skin cells, activating and regenerating skin cells, accelerating wound healing and tissue repair, relieving skin redness and resisting erythra; the moisturizing factor can increase the moisturizing property, elasticity and smoothness of the skin, make the skin smooth and tender, and recover and keep a light state.

The composition containing the medlar stem cells and capable of improving the skin firmness can activate muscle bottom cells and accelerate the metabolism of the skin; supplementing moisture to the skin, increasing the moisture retention of the skin and improving the skin firmness; the collagen synthesis is promoted, the skin relaxation is improved, the skin tightness is improved by the triple mechanism from inside to outside, and the skin tightening contour is reshaped. Stimulating genes of extracellular matrix through intercellular communication, and improving collagen and elastin; the outer layer replenishes water, the inner layer locks water, and the moisturizing effect is exerted from inside to outside; the outside is compacted, and inside is filled, promotes skin compactness from inside to outside.

Drawings

FIG. 1 is a graph showing the effect of promoting collagen.

Figure 2 is a graph comparing the increase in skin firmness to that before testing.

Detailed Description

The proportion of the examples and the comparative examples is as follows:

taking example 1 as an example, the preparation method is applied, and 0.03 part of xanthan gum, 0.3 part of sodium polyacryloyldimethyl taurate, 3 parts of glycerol, 4 parts of propylene glycol, 0.3 part of carboxymethyl chitosan, 0.5 part of preservative PEHG and a proper amount of deionized water are added, and the steps are as follows:

And (3) verifying the synthesis efficacy of the collagen:

proline involved in collagen synthesis is used³Labeled with H, human dermal fibroblasts (NHDF) containing 10. mu. mol/L concentration of each sample were added and cultured for 24 hours. Then, the collagen fraction was taken out, and the absorption of the collagen fraction was measured by a liquid scintillation counter and a slot blotting apparatus³And H amount. As shown in fig. 1, collagen synthesis was significantly promoted.

And (4) conclusion: as can be seen from the data in FIG. 1, the composition provided by the present invention significantly promotes collagen synthesis.

Increase skin firmness:

50 women, 40-65 years old, with signs of facial skin laxity, were selected and the samples provided in examples 1-3 and comparative examples 1-3 were used half-face 2 times a day for 28 days. The skin firmness (epidermis + dermis) was measured using an ultrasonic tester, and the change in skin firmness before and after use was measured.

And (4) conclusion: as can be seen from the data in fig. 2, the compositions provided by the present invention significantly increased skin firmness.

Claims

1. The composition containing the medlar stem cells and capable of improving skin firmness is characterized by comprising the following components in parts by weight: 0.1-0.5 part of medlar extract, 2-10 parts of lactobacillus fermentation product, 0.1-0.8 part of nano biological firming agent, 0.05-0.2 part of acetylated sodium hyaluronate, 0.1-0.5 part of phenoxyethanol, 0.8-2 parts of 1,2 pentanediol, 0.5-2 parts of glycerol glucoside, 8-18 parts of schizosaccharomyces cerevisiae fermentation filtrate, 0.2-0.6 part of tocopherol acetate, 0.1-0.5 part of hydrogenated lecithin and 0.5-1 part of squalane.

2. The composition containing the lycium barbarum stem cells capable of improving skin firmness according to claim 1, characterized by comprising the following ingredients in parts by weight: 0.28 part of medlar extract, 6.5 parts of lactobacillus fermentation product, 0.6 part of nano biological firming agent, 0.12 part of acetylated sodium hyaluronate, 0.35 part of phenoxyethanol, 1.5 parts of 1,2 pentanediol, 1.5 parts of glycerol glucoside, 12 parts of yeast fermentation filtrate, 0.35 part of tocopheryl acetate, 0.5 part of hydrogenated lecithin and 0.8 part of squalane.

3. The composition containing the lycium barbarum stem cells capable of improving skin firmness according to claim 1, characterized by comprising the following ingredients in parts by weight: 0.1 part of medlar extract, 2 parts of lactobacillus fermentation product, 0.1 part of nano biological firming agent, 0.05 part of acetylated sodium hyaluronate, 0.1 part of phenoxyethanol, 0.8 part of 1,2 pentanediol, 0.5 part of glycerol glucoside, 8 parts of yeast schizophyllum fermentation filtrate, 0.2 part of tocopheryl acetate, 0.1 part of hydrogenated lecithin and 0.5 part of squalane.

4. The composition containing the lycium barbarum stem cells capable of improving skin firmness according to claim 1, characterized by comprising the following ingredients in parts by weight: 0.5 part of medlar extract, 10 parts of lactobacillus fermentation product, 0.8 part of nano biological firming agent, 0.2 part of acetylated sodium hyaluronate, 0.5 part of phenoxyethanol, 2 parts of 1,2 pentanediol, 2 parts of glycerol glucoside, 18 parts of yeast fermentation filtrate, 0.6 part of tocopherol acetate, 0.5 part of hydrogenated lecithin and 1 part of squalane.

5. The composition containing the Lycium barbarum stem cells capable of improving skin firmness according to any one of claims 1-4, wherein the Lycium barbarum extract is a culture extract of Lycium barbarum stem cells.

6. The application of the composition containing the medlar stem cells and capable of improving the skin firmness is characterized by comprising the following steps: