CN112805388A - 微生物的检测方法 - Google Patents
微生物的检测方法 Download PDFInfo
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- CN112805388A CN112805388A CN201980066504.4A CN201980066504A CN112805388A CN 112805388 A CN112805388 A CN 112805388A CN 201980066504 A CN201980066504 A CN 201980066504A CN 112805388 A CN112805388 A CN 112805388A
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| JP2018-205797 | 2018-10-31 | ||
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000279199A (ja) * | 1999-02-03 | 2000-10-10 | Ortho Clinical Diagnostics Inc | 血清又は血漿からｄｎａを調製するための改良法 |
| CN101849024A (zh) * | 2007-11-05 | 2010-09-29 | 荣研化学株式会社 | 核酸扩增用样品的制备方法及制备试剂盒 |
| CN102559861A (zh) * | 2010-12-30 | 2012-07-11 | 上海复星医学科技发展有限公司 | 沙眼衣原体核酸快速检测试剂盒 |
| CN105349661A (zh) * | 2015-11-27 | 2016-02-24 | 湖南圣湘生物科技有限公司 | 一种沙眼衣原体/淋球菌核酸检测试剂盒 |
| JP2017060413A (ja) * | 2015-09-24 | 2017-03-30 | 東洋紡株式会社 | 核酸分析用のサンプル調製方法 |
| CN107250377A (zh) * | 2014-11-25 | 2017-10-13 | 奎斯特诊断投资股份有限公司 | 生物样品中的核酸的扩增和检测 |
| WO2017180745A1 (en) * | 2016-04-14 | 2017-10-19 | T2 Biosystems, Inc. | Methods and systems for amplification in complex samples |
| WO2018168986A1 (ja) * | 2017-03-15 | 2018-09-20 | 東洋紡株式会社 | 遺伝子検査方法及び遺伝子検査キット |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0377573A1 (en) * | 1987-07-22 | 1990-07-18 | Imperial Cancer Research Technology Limited | Digestion method |
| JP4852807B2 (ja) * | 2001-08-21 | 2012-01-11 | Ｊｓｒ株式会社 | ウイルス検出方法 |
| JP2003310265A (ja) * | 2002-04-22 | 2003-11-05 | Eiken Chem Co Ltd | 核酸抽出法 |
| JP4301486B2 (ja) * | 2002-06-27 | 2009-07-22 | 栄研化学株式会社 | 核酸抽出法 |
| CN110257477A (zh) * | 2011-04-27 | 2019-09-20 | 北京大学 | 核酸液相提取及检测方法 |
| JP2014000015A (ja) * | 2012-06-15 | 2014-01-09 | Kaneka Corp | 核酸解析法 |
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Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000279199A (ja) * | 1999-02-03 | 2000-10-10 | Ortho Clinical Diagnostics Inc | 血清又は血漿からｄｎａを調製するための改良法 |
| US6268127B1 (en) * | 1999-02-03 | 2001-07-31 | Ortho-Clinical Diagnostics, Inc. | Method for preparing DNA from serum and plasma |
| CN101849024A (zh) * | 2007-11-05 | 2010-09-29 | 荣研化学株式会社 | 核酸扩增用样品的制备方法及制备试剂盒 |
| CN102559861A (zh) * | 2010-12-30 | 2012-07-11 | 上海复星医学科技发展有限公司 | 沙眼衣原体核酸快速检测试剂盒 |
| CN107250377A (zh) * | 2014-11-25 | 2017-10-13 | 奎斯特诊断投资股份有限公司 | 生物样品中的核酸的扩增和检测 |
| JP2017060413A (ja) * | 2015-09-24 | 2017-03-30 | 東洋紡株式会社 | 核酸分析用のサンプル調製方法 |
| CN105349661A (zh) * | 2015-11-27 | 2016-02-24 | 湖南圣湘生物科技有限公司 | 一种沙眼衣原体/淋球菌核酸检测试剂盒 |
| WO2017180745A1 (en) * | 2016-04-14 | 2017-10-19 | T2 Biosystems, Inc. | Methods and systems for amplification in complex samples |
| WO2018168986A1 (ja) * | 2017-03-15 | 2018-09-20 | 東洋紡株式会社 | 遺伝子検査方法及び遺伝子検査キット |
Non-Patent Citations (3)
| Title |
|---|
| CAMPOS PF: "DNA extraction from formalin-fixed material", METHODS MOL BIOL, 14 November 2012 (2012-11-14) * |
| KIM SA: "An efficient and reliable DNA extraction method for preimplantation genetic diagnosis: a comparison of allele drop out and amplification rates using different single cell lysis methods", FERTIL STERIL, 31 August 2009 (2009-08-31) * |
| SJÖHOLM MI: "Assessing quality and functionality of DNA from fresh and archival dried blood spots and recommendations for quality control guidelines", CLIN CHEM, 31 August 2007 (2007-08-31) * |
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| WO2020075696A1 (ja) | 2020-04-16 |
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