CN112753850A - Preparation method of insect protein source biological feed - Google Patents
Preparation method of insect protein source biological feed Download PDFInfo
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- 108010060231 Insect Proteins Proteins 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 36
- 230000004151 fermentation Effects 0.000 claims abstract description 36
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 35
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 35
- 239000011669 selenium Substances 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 32
- 239000004576 sand Substances 0.000 claims abstract description 22
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 16
- 239000011259 mixed solution Substances 0.000 claims abstract description 16
- 235000007340 Hordeum vulgare Nutrition 0.000 claims abstract description 15
- 241000254109 Tenebrio molitor Species 0.000 claims abstract description 15
- 239000011550 stock solution Substances 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 210000003608 fece Anatomy 0.000 claims abstract description 12
- 150000004666 short chain fatty acids Chemical class 0.000 claims abstract description 12
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 11
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 11
- 241000018646 Pinus brutia Species 0.000 claims abstract description 11
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 10
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 10
- 229940073490 sodium glutamate Drugs 0.000 claims abstract description 10
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 240000008042 Zea mays Species 0.000 claims abstract description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 5
- 235000005822 corn Nutrition 0.000 claims abstract description 5
- 230000003068 static effect Effects 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 240000005979 Hordeum vulgare Species 0.000 claims abstract 3
- 238000000034 method Methods 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 10
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 9
- 239000000835 fiber Substances 0.000 claims description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- 241000709785 Hermetia illucens Species 0.000 claims description 3
- 241001233061 earthworms Species 0.000 claims description 3
- 239000003205 fragrance Substances 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 description 22
- 235000013372 meat Nutrition 0.000 description 19
- 239000000796 flavoring agent Substances 0.000 description 15
- 235000019634 flavors Nutrition 0.000 description 15
- 229940088598 enzyme Drugs 0.000 description 14
- 241000209219 Hordeum Species 0.000 description 12
- 241000238631 Hexapoda Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 230000006872 improvement Effects 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 229940024606 amino acid Drugs 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 239000006041 probiotic Substances 0.000 description 6
- 235000018291 probiotics Nutrition 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 229920002101 Chitin Polymers 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 230000002458 infectious effect Effects 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 210000003205 muscle Anatomy 0.000 description 4
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- 235000013305 food Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
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- 239000000126 substance Substances 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- 241000533950 Leucojum Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229940079919 digestives enzyme preparation Drugs 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 150000004665 fatty acids Chemical group 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 229940088592 immunologic factor Drugs 0.000 description 2
- 239000000367 immunologic factor Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000003871 intestinal function Effects 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 235000020997 lean meat Nutrition 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
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- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
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- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 241001481656 Stratiomyidae Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
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- 239000004220 glutamic acid Substances 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
Abstract
The invention discloses a preparation method of insect protein source biological feed, which comprises the following steps: s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate; s2, adding the raw materials into water in an amount which is 5-15 times the weight of the raw materials to form a mixed solution, and standing the mixed solution; s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture; s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days. The invention is applied to the technical field of biological feed.
Description
Technical Field
The invention relates to the technical field of biological feeds, in particular to a preparation method of an insect protein source biological feed.
Background
In the prior art, the traditional biological feed has high antibiotic content, single nutrient content and high preparation cost.
Disclosure of Invention
Technical problem to be solved
A method for preparing insect protein source biological feed, which solves the technical problems of high antibiotic content, single nutrient content and high preparation cost.
(II) technical scheme
In order to solve the technical problems, the invention provides a preparation method of insect protein source biological feed, which comprises the following steps:
s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate;
s2, adding the raw materials into water which is 5-15 times the weight ratio of the raw materials to form a mixed solution, and standing the mixed solution in an environment with the temperature of 28-30 ℃ and the air humidity of 65-75%;
s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture;
s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days.
In a further improvement, in step S1, the content of organic selenium in the selenium-enriched yeast is 2000-2400 mg/kg.
In a further improvement, in step S1, the short chain fatty acid and the sodium glutamate are respectively food grade.
In a further improvement, in step S1, the strain in the strain stock solution is yeast or lactic acid bacteria.
In a further modification, in step S2, each raw material is added to 10 times by weight of water to form a mixed solution.
In a further modification, in step S2, the mixed solution is allowed to stand for 1 hour in an environment with a temperature of 28 to 30 ℃ and an air humidity of 65 to 75%.
In a further improvement, in step S3, the method for making the ferment residue includes the following steps:
grinding one or more of hermetia illucens, yellow mealworms, barley worms and earthworms into worm pulp, mixing the worm pulp, the biological enzyme and the fermentation strain for fermentation, and obtaining worm pulp enzyme and worm pulp enzyme residues after fermentation.
In a further improvement, in step S4, the mixture after standing and the mixture are mixed uniformly according to the weight ratio of 1, and then the mixture is put into a fermentation vat for fermentation.
In a further improvement, in step S4, when sweet smell and white hypha are generated by fermentation, the fermented hypha is a feed finished product, and then the feed finished product is sequentially detected and packaged.
In a further improvement, in step S4, during the sub-packaging, the finished feed is packaged by using a breathing bag with a breathing valve, and a fiber bag is sleeved outside the breathing bag.
(III) advantageous effects
The preparation method of the insect protein source biological feed can fully and reasonably utilize the enzyme residues, and can reduce the manufacturing cost of the insect protein source biological feed. The ferment residues are rich in micromolecular peptides, antibacterial peptides, chitin, various trace elements, beneficial bacteria, enzyme preparations, animal and plant growth factors, vitamins, amino acids and the like which are rich in ferment, so that the prepared insect protein source biological feed is balanced and comprehensive in nutrition. A large amount of active insect antibacterial small peptides and probiotic bacteria are reserved in the enzyme residues, and the derivatives of the enzyme residues also contain a large amount of immune factors, so that the immunity of the organism can be greatly improved, the morbidity of cultured animals can be well prevented in the culture of the animals, and the survival rate of the animals is greatly improved.
The selenium-enriched yeast is rich in organic selenium, so that the organic selenium of animals can be improved, and the production of green pollution-free products is realized by using the organic selenium in the feed instead of antibiotics. And secondly, the short-chain fatty acid, pine needle powder and the like capable of improving fiber and fat between fleshy muscles are provided by the selenium-enriched yeast rich in organic selenium, so that a safe selenium source product without toxic and side effects is provided for enterprises breeding high-end selenium-enriched agricultural products, the healthy growth of animals is promoted, and a reliable choice is provided for the enterprises to produce high-quality selenium-enriched agricultural products integrating health and delicious taste.
The insect source biological feed can greatly improve the concentration and the activity of probiotic groups by adding the yellow mealworm excrement sand and the barley worm excrement sand, and the yellow mealworm excrement sand and the barley worm excrement sand are not only high in protein content but also belong to hollow porous structures, so that the insect source biological feed is very suitable for the propagation of probiotics and has higher space density. Therefore, the insect protein source biological feed can also decompose residual bait and excrement in a farm and degrade harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide, has good control effects on purifying the culture environment and inhibiting harmful bacteria, and can also greatly improve the conversion rate of the feed and reduce the cost of the feed. The tenebrio molitor dung sand and the barley pest dung sand can make up respective defects, promote respective advantages, well improve meat taste and flavor of economically cultured animals, have different amino acid ratios and fatty acid structures, and can better meet the requirements of the animals on specific amino acid through proportioning.
The short-chain fatty acid can increase the amount of intramuscular fat and intramyofiber of the meat, and can increase the meat-feeling elasticity and the fragrant and sweet flavor of the meat; the sodium glutamate can improve the delicate flavor and sweet taste of meat; the pine needle powder contains pine ester component capable of improving meat quality and flavor. The insect powder contains a large amount of chitin and shrimp shell powder, and the brittleness and toughness of the fish skin can be greatly improved.
The insect protein source biological feed prepared by the preparation method of the insect protein source biological feed can be preserved for more than one year, while the insect protein source biological feed in the prior art can be preserved for only 5 to 7 days. Can improve meat quality and flavor of animal, increase fiber and fat content of animal muscle, and increase lean meat percentage and snowflake meat ratio and flavor. Can prevent various diseases, improve intestinal function, improve feed utilization rate, improve production performance, improve meat quality and flavor, and can be used as selenium source additive for cultivating selenium-rich meat.
The preparation method of the insect protein source biological feed adopts the strain stock solution which can be fermented in advance and can also be obtained by other methods. When the fermentation is carried out in the step S4, the success rate of the fermentation can be improved, and the risk of infectious microbe infection is reduced, because the insect protein in the strain stock solution belongs to high-protein animal raw materials, the infectious microbes are easier to breed. Therefore, the fermented strain stock solution is directly used for secondary fermentation in the step S4 and other raw materials, so that the produced insect protein source biological feed is safer, the storage time is longer, and the quality is more stable.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
A preparation method of insect protein source biological feed comprises the following steps:
s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate;
s2, adding the raw materials into water which is 5-15 times the weight ratio of the raw materials to form a mixed solution, and standing the mixed solution in an environment with the temperature of 28-30 ℃ and the air humidity of 65-75%;
s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture;
s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days.
Specifically, S1, the following first raw materials are selected according to the weight ratio: 13 parts of strain stock solution, 55 parts of brown sugar, 25 parts of selenium-enriched yeast, 13 parts of short-chain fatty acid and 8 parts of sodium glutamate;
s2, adding the raw materials into water which is 10 times of the raw materials by weight to form a mixed solution, and standing the mixed solution in an environment with the temperature of 29 ℃ and the air humidity of 70%;
s3, selecting the following second raw materials in percentage by weight: 25 parts of enzyme residues, 25 parts of yellow mealworm excrement sand, 25 parts of barley worm excrement sand, 8 parts of pine needle powder and 13 parts of corn powder, and mixing the second raw materials to form a mixture;
s4, uniformly mixing the static mixed liquor and the mixture according to the weight ratio of 2, and then fermenting, wherein the fermentation process parameters are as follows: the temperature is 29 ℃, the air humidity is 70%, and the fermentation time is 4 days.
According to the preparation method of the insect protein source biological feed, the ferment residues can be reasonably utilized fully and reasonably, and meanwhile, the manufacturing cost of the insect protein source biological feed can be reduced. The ferment residues are rich in micromolecular peptides, antibacterial peptides, chitin, various trace elements, beneficial bacteria, enzyme preparations, animal and plant growth factors, vitamins, amino acids and the like which are rich in ferment, so that the prepared insect protein source biological feed is balanced and comprehensive in nutrition. A large amount of active insect antibacterial small peptides and probiotic bacteria are reserved in the enzyme residues, and the derivatives of the enzyme residues also contain a large amount of immune factors, so that the immunity of the organism can be greatly improved, the morbidity of cultured animals can be well prevented in the culture of the animals, and the survival rate of the animals is greatly improved.
The selenium-enriched yeast is rich in organic selenium, so that the organic selenium of animals can be improved, and the production of green pollution-free products is realized by using the organic selenium in the feed instead of antibiotics. And secondly, the short-chain fatty acid, pine needle powder and the like capable of improving fiber and fat between fleshy muscles are provided by the selenium-enriched yeast rich in organic selenium, so that a safe selenium source product without toxic and side effects is provided for enterprises breeding high-end selenium-enriched agricultural products, the healthy growth of animals is promoted, and a reliable choice is provided for the enterprises to produce high-quality selenium-enriched agricultural products integrating health and delicious taste.
The insect source biological feed of the embodiment can greatly improve the concentration and the activity of the probiotic groups by adding the yellow mealworm excrement sand and the barley pest excrement sand, and the yellow mealworm excrement sand and the barley pest excrement sand are not only high in protein content but also belong to hollow porous structures, so that the insect source biological feed is very suitable for the propagation of probiotics and is higher in space density. Therefore, the insect protein source biological feed can also decompose residual bait and excrement in a farm and degrade harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide, has good control effects on purifying the culture environment and inhibiting harmful bacteria, and can also greatly improve the conversion rate of the feed and reduce the cost of the feed. The tenebrio molitor dung sand and the barley pest dung sand can make up respective defects, promote respective advantages, well improve meat taste and flavor of economically cultured animals, have different amino acid ratios and fatty acid structures, and can better meet the requirements of the animals on specific amino acid through proportioning.
The short-chain fatty acid can increase the amount of intramuscular fat and intramyofiber of the meat, and can increase the meat-feeling elasticity and the fragrant and sweet flavor of the meat; the sodium glutamate can improve the delicate flavor and sweet taste of meat; the pine needle powder contains pine ester component capable of improving meat quality and flavor. The insect powder contains a large amount of chitin and shrimp shell powder, and the brittleness and toughness of the fish skin can be greatly improved.
The insect protein source biological feed prepared by the preparation method of the insect protein source biological feed can be preserved for more than one year, while the insect protein source biological feed in the prior art can be preserved for only 5-7 days. Can improve meat quality and flavor of animal, increase fiber and fat content of animal muscle, and increase lean meat percentage and snowflake meat ratio and flavor. Can prevent various diseases, improve intestinal function, improve feed utilization rate, improve production performance, improve meat quality and flavor, and can be used as selenium source additive for cultivating selenium-rich meat.
In the preparation method of the insect protein source biological feed, the adopted strain stock solution can be fermented in advance, and can also be obtained by other methods. When the fermentation is carried out in the step S4, the success rate of the fermentation can be improved, and the risk of infectious microbe infection is reduced, because the insect protein in the strain stock solution belongs to high-protein animal raw materials, the infectious microbes are easier to breed. Therefore, the fermented strain stock solution is directly used for secondary fermentation in the step S4 and other raw materials, so that the produced insect protein source biological feed is safer, the storage time is longer, and the quality is more stable.
Further, in one embodiment, in the step S1, the content of organic selenium in the selenium-enriched yeast is 2000-2400 mg/kg. Specifically, in step S1, the selenium-enriched yeast contains 2200mg/kg of organic selenium.
Further, in one embodiment, in step S1, the short chain fatty acid and the sodium glutamate are food grade, and the food grade is edible grade.
Further, in an embodiment, in step S1, the strain in the strain stock solution is yeast or lactic acid bacteria, which can well inhibit the growth and reproduction of other harmful bacteria.
Further, in one embodiment, in step S2, each raw material is added to 10 times by weight of water to form a mixed solution.
Further, in one embodiment, in step S2, the mixed solution is allowed to stand for 1 hour in an environment with a temperature of 28 to 30 ℃ and an air humidity of 65 to 75%. Specifically, in step S2, the mixed solution is allowed to stand for 1 hour in an environment at a temperature of 29 ℃ and an air humidity of 70%.
Further, in an embodiment, in step S3, the method for manufacturing the ferment slag includes the following steps:
grinding one or more of hermetia illucens, yellow mealworms, barley worms and earthworms into worm pulp, mixing the worm pulp, the biological enzyme and the fermentation strain for fermentation, and obtaining worm pulp enzyme and worm pulp enzyme residues after fermentation. The contents of the protein in the pulp ferment residues of yellow mealworms, barley worms, black soldier flies and the like are high, and the contents of substances such as glutamic acid which influences the meat quality and the delicate flavor are more abundant.
Further, in an embodiment, in step S4, the mixture after standing and the mixture are mixed uniformly according to the weight ratio of 1, and then the mixture is placed into a fermentation barrel for fermentation, which is convenient for fermentation.
Further, in an embodiment, in step S4, when the sweet smell and white hypha are generated by fermentation, it indicates that the fermented hypha is a finished feed product, and then the finished feed product is sequentially detected and packaged. The fermentation condition can be observed conveniently and timely, and abnormal conditions can be treated conveniently and timely.
Further, in an embodiment, in step S4, during the sub-packaging, the finished feed is packaged by using a breathing bag with a breathing valve, and a fiber bag is sleeved outside the breathing bag for packaging, transporting, storing and the like.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A preparation method of insect protein source biological feed is characterized by comprising the following steps:
s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate;
s2, adding the raw materials into water which is 5-15 times the weight ratio of the raw materials to form a mixed solution, and standing the mixed solution in an environment with the temperature of 28-30 ℃ and the air humidity of 65-75%;
s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture;
s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days.
2. The method for preparing the insect protein-derived biological feed according to claim 1, wherein in step S1, the content of organic selenium in the selenium-enriched yeast is 2000-2400 mg/kg.
3. The method for preparing an insect protein-derived biological feed according to claim 1, wherein in step S1, the short-chain fatty acid and the sodium glutamate are food-grade.
4. The method for preparing an insect protein-derived biological feed according to claim 1, wherein in step S1, the strain in the strain stock solution is yeast or lactic acid bacteria.
5. The method of claim 1, wherein in step S2, the raw materials are added to 10 times the weight of water to form a mixture.
6. The method for preparing an insect protein-derived biological feed according to claim 1, wherein the mixed solution is allowed to stand for 1 hour in an environment having a temperature of 28 to 30 ℃ and an air humidity of 65 to 75% in step S2.
7. The method for preparing the insect protein-derived biological feed according to claim 1, wherein the method for preparing the ferment residues in step S3 comprises the following steps:
grinding one or more of hermetia illucens, yellow mealworms, barley worms and earthworms into worm pulp, mixing the worm pulp, the biological enzyme and the fermentation strain for fermentation, and obtaining worm pulp enzyme and worm pulp enzyme residues after fermentation.
8. The method for preparing an insect protein-derived biological feed according to claim 1, wherein in step S4, the mixture after standing and the mixture are mixed uniformly in a weight ratio of 1 and then placed in a fermentation vat for fermentation.
9. The method for preparing an insect protein-derived biological feed according to any one of claims 1 to 8, wherein the fermentation in step S4 produces sweet smell and white hyphae, which indicates that the feed is fermented into a finished product, and the finished product is sequentially detected and packaged.
10. The method for preparing a biological feed derived from insect proteins as claimed in claim 9, wherein in step S4, the finished feed is packaged by using a breathing bag with a breathing valve, and the breathing bag is covered with a fiber bag.
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