CN112703255B - 化学组合物及其使用方法 - Google Patents

化学组合物及其使用方法 Download PDF

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Publication number
CN112703255B
CN112703255B CN201980047067.1A CN201980047067A CN112703255B CN 112703255 B CN112703255 B CN 112703255B CN 201980047067 A CN201980047067 A CN 201980047067A CN 112703255 B CN112703255 B CN 112703255B
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Prior art keywords
nucleic acid
domain
acid molecule
detectable label
nucleotides
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Chinese (zh)
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CN112703255A (zh
Inventor
D·杜纳威
E·曼罗
J·比彻姆
R·哈菲佐夫
S·科鲁孔达
Y·邓
D·金
M·格雷戈里
M·黄
M·沃尔什
G·梅雷迪斯
M·麦克尔文
P·斯基尼
C·伯克
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Brook Space Biology Co
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Brook Space Biology Co
Nanostring Technologies Inc
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Priority to CN202510475216.1A priority Critical patent/CN120350103A/zh
Publication of CN112703255A publication Critical patent/CN112703255A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980047067.1A 2018-05-14 2019-05-14 化学组合物及其使用方法 Active CN112703255B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202510475216.1A CN120350103A (zh) 2018-05-14 2019-05-14 化学组合物及其使用方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862671091P 2018-05-14 2018-05-14
US62/671091 2018-05-14
US201962836327P 2019-04-19 2019-04-19
US62/836327 2019-04-19
PCT/US2019/032168 WO2019222178A1 (en) 2018-05-14 2019-05-14 Chemical compositions and methods of using same

Related Child Applications (1)

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Publications (2)

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CN112703255A CN112703255A (zh) 2021-04-23
CN112703255B true CN112703255B (zh) 2025-04-29

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US (3) US11549139B2 (https=)
EP (2) EP3794146B1 (https=)
JP (2) JP7525402B2 (https=)
KR (2) KR20250171474A (https=)
CN (2) CN112703255B (https=)
AU (2) AU2019271028B2 (https=)
CA (1) CA3099909A1 (https=)
SG (1) SG11202011274YA (https=)
WO (1) WO2019222178A1 (https=)

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EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
US12492427B2 (en) 2020-06-18 2025-12-09 Resolve Biosciences Gmbh Multiplex method for detecting different analytes in a sample
CA3192943A1 (en) * 2020-09-16 2022-03-24 Nanostring Technologies, Inc. Chemical compositions and methods of using the same
US20220316004A1 (en) * 2021-04-06 2022-10-06 Akoya Biosciences, Inc. Detection of labeled analytes in biological samples
EP4216220A1 (en) * 2022-01-25 2023-07-26 Leica Microsystems CMS GmbH Data storage device and method for storing data
EP4680764A2 (en) 2023-03-17 2026-01-21 Bruker Spatial Biology, Inc. Assay for recombinase accessible chromatin and related compositions and methods
WO2024196728A2 (en) 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Nucleic acid probes for combined sequencing and spatial analysis
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method
CN119932194A (zh) * 2025-01-23 2025-05-06 上海市第十人民医院 一种用于靶标rna多重分析的共价荧光编码组装体、dna荧光条形码及应用

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Publication number Priority date Publication date Assignee Title
CN107208144A (zh) * 2014-11-21 2017-09-26 纳米线科技公司 无酶且无扩增的测序

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