CN112587472A

CN112587472A - Preparation method of osmunda japonica and face tightening eye cream containing apple cell extract

Info

Publication number: CN112587472A
Application number: CN202011509790.8A
Authority: CN
Inventors: 张应贤
Original assignee: Guangzhou Lakel Stem Cell Research Institute
Current assignee: Guangzhou Lakel Stem Cell Research Institute
Priority date: 2020-12-19
Filing date: 2020-12-19
Publication date: 2021-04-02

Abstract

The invention discloses a preparation method of osmunda japonica and face firming eye cream containing an apple cell extract, and relates to the technical field of skin care products. Preparing raw materials: 0.005-0.01% of apple cell extract, 1.6-6% of active extract, 2-5% of instant effect promoting firming agent, 3-7% of vegetable fat, 80.1-0.5% of acetyl hexapeptide, 0.85-2% of antioxidant, 1-3% of skin feeling conditioner, 4.21-13.23% of humectant, 0.5-1% of preservative, 1-5% of emulsifier, 1-2.5% of emollient, 1-2% of surfactant, 0.5-1% of thickener, 0.1-0.2% of essence and 80-85% of deionized water. The antioxidant and the active extract added in the invention supplement collagen for eye cream, repair fine lines and eye bags, tighten the whole eye contour, realize deep tightening, recover young spirit, effectively resist upper eyelid laxity and fishtail lines, make double eyelids more obvious, also can reduce swelling, scratch small fine lines, drive off dim fine lines, make skin like a soaked sponge, improve absorption capacity to the maximum extent, endow skin elasticity again, and realize eye skin tightening and smooth wrinkles.

Description

Preparation method of osmunda japonica and face tightening eye cream containing apple cell extract

Technical Field

The invention relates to the technical field of skin care products, in particular to a preparation method of a osmunda japonica and facial tightening eye cream containing an apple cell extract.

Background

The modern young people always take the most expensive eye cream after staying up at the latest night, take chicken for pursuing a drama in the late night and learn overtime after staying up at night, and various skin problems follow the chicken, wherein the problems of fine lines and black eyes on eyes are most obvious. The eye cream is a skin care cosmetic product, has the effects of moistening and tightening the eye skin, can relieve the problems of dark circles and under-eye puffiness, and has the effects of improving wrinkles and fine lines.

The existing eye cream has a common moisturizing effect in the using process, is not easy to spread in the using process, has a common absorption speed, has a common wrinkle smoothing effect and a common invisible pore effect, and further cannot effectively cover wrinkles.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention provides a preparation method of an osmunda face tightening eye cream containing an apple cell extract, which aims to solve the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a method for preparing Osmunda face tightening eye cream containing apple cell extract comprises the following steps:

s1, preparing raw materials: 0.005-0.01% of apple cell extract, 1.6-6% of active extract, 2-5% of instant effect promoting firming agent, 3-7% of vegetable fat, 80.1-0.5% of acetyl hexapeptide, 0.85-2% of antioxidant, 1-3% of skin feel conditioner, 4.21-13.23% of humectant, 0.5-1% of preservative, 1-5% of emulsifier, 1-2.5% of emollient, 1-2% of surfactant, 0.5-1% of thickener, 0.1-0.2% of essence and 80-85% of deionized water;

s2, preparation of phase A: sequentially adding the thickening agent, the deionized water and the humectant in the step S1 into an emulsifying pot, stirring and heating to 80-85 ℃, dissolving completely, homogenizing properly for 1-3min, and keeping the temperature for later use;

s3, preparation of phase B: dispersing the instant effect promoting firming agent, the emollient, the skin feeling conditioner and the surfactant in the step S1 by using an ultrasonic dispersion machine, sequentially adding the emulsifier, the vegetable oil, the active extract and the essence into the dispersed raw materials, heating and stirring to 80 ℃ to prepare a phase B for later use;

s4, mixing of raw materials: adding the phase B in the step S3 into the phase A in the step S2, homogenizing for 3-5min, vacuumizing, and keeping the temperature and stirring for 15-25min for later use;

s5, preparation of eye cream: cooling the raw materials obtained in the step S4, then sequentially adding a preservative, an antioxidant, an apple cell extract and acetyl hexapeptide-8, and uniformly stirring to obtain the required eye cream;

s6, sampling inspection, packaging and discharging: and (5) sampling, inspecting, packaging and discharging the eye cream prepared in the step S5.

Further optimizing the technical scheme, the humectant in the step S2 comprises polyquaternium-511-2.5%, glycerol 1-5%, betaine 0.1-0.5%, allantoin 0.1-0.2%, 1, 3-butanediol 2-5% and sodium hyaluronate 0.01-0.03%, and the heat preservation time in the step S2 is 8-10 min.

Further optimizing the technical scheme, the thickener in the step S2 includes two or more of xanthan gum, VP/hexadecene copolymer, sclerotium rolfsii gum, pullulanase polysaccharide, carbomer and polyacryl dimethyl ammonium taurate.

Further optimizing the technical scheme, the active extract in the step S3 comprises 0.5-1.5% of sargassum tonkinensis extract, 0.5-1.5% of algae extract (nannochloropsis sp. extract), 0.1-1% of spilanthol/leaf/stem extract and 0.5-2% of astragalus membranaceus root extract.

Further optimizing the technical solution, the emulsifier in step S3 includes two or more of lecithin, cetearyl alcohol, glyceryl stearate, glyceryl oleate, cetyl alcohol, steareth-20, and polysorbate-40.

Further optimizing the technical scheme, the skin feel conditioning agent in the step S3 comprises two or more of silica, nylon-12, potassium cocoyl hydrolyzed oat protein, methyl glucitol polyether-20 and glycerol polyacrylate.

Further optimizing the technical scheme, the effective lifting tightening agent in the step S3 comprises 1-3% of divinyl polydimethylsiloxane/polydimethylsiloxane cross-linked polymer and 1-2% of hydroxypropyl cyclodextrin.

Further optimizing the technical scheme, the vegetable oil in the step S3 includes three or more of jojoba seed oil, safflower seed oil, sunflower seed oil, corn oil, sesame seed oil, macadamia ternifolia seed oil, olive oil, sunflower seed wax, and cercis chinensis flower wax.

Further optimizing the technical scheme, the antioxidant in the step S5 comprises 0.5-1% of fullerene, 0.1-0.3% of sodium benzotriazolyl butylphenolate sulfonate, 0.05-0.1% of pentaerythritol tetra (bis-tert-butyl hydroxy hydrocinnamic acid) ester and 0.2-0.6% of tocopherol (vitamin E).

Further optimizing the technical scheme, the preservative in the step S5 includes two or more of caprylyl glycol, phenoxyethanol, propylparaben, methylparaben, p-hydroxyacetophenone and ethylhexylglycerin.

Further optimizing the technical scheme, the surfactant in the step S3 comprises one or more of PEG-75 stearate, ceteth-20 and PEG-10 polydimethylsiloxane.

In order to further optimize the technical scheme, the emollient in the step S3 includes two or more of squalane, polydimethylsiloxane, polyglycerol-3, cyclopentyldimethylsiloxane, polysiloxane-11, isohexadecane, caprylic/capric triglyceride, glycerol tri (ethyl hexanoate) and isononyl isononanoate.

Further optimizing the technical scheme, the apple cell extract in the step S5 is prepared by adding 75% ethanol into dry apple fruit cell culture powder according to the liquid-solid ratio of 1:2 for pre-soaking for 15min, and performing ultrasonic extraction for 20min each time for 2 times at 25KHz and 300W.

Further optimizing the technical scheme, the vegetable oil and fat in the step S3 is obtained by refining to remove various impurities and free fatty acids.

Advantageous effects

Compared with the prior art, the invention provides a preparation method of the osmunda face tightening eye cream containing the apple cell extract, and the preparation method has the following beneficial effects:

1. the preparation method of the osmunda japonica firming eye cream containing the apple cell extract comprises the steps of adding the apple stem cells to promote a large amount of regeneration of dormant skin stem cells, promoting metabolism, enhancing the elasticity and water-retaining property of skin, reducing wrinkles, enhancing the activity of epidermal cells, improving the spreadability and skin feel by using vegetable oil, softening and lubricating the skin, providing a hydrophobic film for the skin, recovering the activity of the stem cells of the basal layer of the epidermis, maintaining the renewal capacity of the epidermis, improving the skin quality, supplementing small molecular collagen in the skin, deeply caring and firming the skin, bringing instant bright eye, repairing the skin of deep eyes, smoothing the skin, smoothing the silky and smooth, non-sticky, easy spreading, high absorption speed, strong soft focus covering power, smoothing wrinkles and invisible pores, and effectively covering wrinkles, can provide instant wrinkle-removing and tightening effects, lighten and brighten the eyes, and give natural makeup with soft-focus effect.

2. According to the preparation method of the osmund face tightening eye cream containing the apple cell extract, the added antioxidant and the active extract supplement collagen for the eye cream, repair fine lines and eye bags, tighten the overall eye contour, realize deep tightening, recover young and wonderful, effectively resist upper eyelid looseness and fishtail lines, enable double eyelids to be more obvious, further reduce swelling, draw small fine lines, tighten large eyes, pay attention to moisture preservation, pay attention to antioxidation, activate skin cells, lighten dark circles, continuously nourish both eyes, enable skin around the eyes to be tender, prevent dry lines from appearing in advance, complement each other in effect, relieve fatigue pressure, drive dark fine lines away, enable the skin to be as a soaked sponge, maximally improve absorption, enable skin elasticity to be given again, and achieve eye tightening, smooth wrinkles and bright eyes.

Drawings

Fig. 1 is a schematic flow chart of a preparation method of an osmunda face tightening eye cream containing an apple cell extract according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows: referring to fig. 1, the present invention discloses a method for preparing osmunda facial tightening eye cream containing apple cell extract, comprising the following steps:

s2, preparation of phase A: sequentially adding the thickening agent, the deionized water and the humectant in the step S1 into an emulsifying pot, stirring and heating to 80 ℃, dissolving completely, homogenizing properly for 1min, and keeping the temperature for later use;

s4, mixing of raw materials: adding the phase B in the step S3 into the phase A in the step S2, homogenizing for 3min, vacuumizing, and keeping the temperature and stirring for 15min for later use;

As a specific optimization scheme of this embodiment, the humectant in step S2 includes polyquaternium-511%, glycerin 1%, betaine 0.1%, allantoin 0.1%, 1, 3-butanediol 2%, and sodium hyaluronate 0.01%, and the heat preservation time in step S2 is 8 min.

As a specific optimization scheme of this embodiment, the thickener in step S2 includes two or more of xanthan gum, VP/hexadecene copolymer, sclerotium rolfsii, pullulanase polysaccharide, carbomer, and polyacryl dimethyl ammonium taurate.

As a specific optimization scheme of this embodiment, the active extracts in step S3 include sargassum fusiforme extract 0.5%, algae extract (nannochloropsis sp) 0.5%, inula japonica/leaf/stem extract 0.1%, and astragalus membranaceus root extract 0.5%.

As a specific optimization scheme of this embodiment, the emulsifier in step S3 includes two or more of lecithin, cetearyl alcohol, glyceryl stearate, glyceryl oleate, cetyl alcohol, steareth-20, and polysorbate-40.

As a specific optimization scheme of this embodiment, the skin feel conditioning agent in step S3 includes two or more of silica, nylon-12, potassium cocoyl hydrolyzed oat protein, methyl gluceth-20, and glycerin polyacrylate.

As a specific optimization scheme of this embodiment, the effective lifting and tightening agent in step S3 includes 1-3% of divinyl dimethicone/dimethicone crosspolymer and 1-2% of hydroxypropyl cyclodextrin.

As a specific optimization scheme of this embodiment, the vegetable oil and fat in step S3 includes three or more of jojoba seed oil, safflower seed oil, sunflower seed oil, corn oil, sesame seed oil, macadamia ternifolia seed oil, olive oil, sunflower seed wax, and cercis chinensis flower wax.

As a specific optimization scheme of this embodiment, the antioxidant in step S5 includes 0.5% of fullerene, 0.1% of sodium benzotriazolyl styrene-butadiene-phenol sulfonate, 0.05% of pentaerythritol tetrakis (bis-tert-butyl hydroxyhydrocinnamate) and 0.2% of tocopherol (vitamin E).

As a specific optimization scheme of this embodiment, the preservative in step S5 includes two or more of caprylyl glycol, phenoxyethanol, propylparaben, methylparaben, p-hydroxyacetophenone, and ethylhexylglycerin.

As a specific optimization scheme of this embodiment, the surfactant in step S3 includes one or more of PEG-75 stearate, ceteth-20, and PEG-10 polydimethylsiloxane.

As a specific optimization scheme of this embodiment, the emollient in step S3 includes two or more of squalane, polydimethylsiloxane, polyglycerol-3, cyclopentyldimethylsiloxane, polysiloxane-11, isohexadecane, caprylic/capric triglyceride, glycerol tris (ethylhexanoate), and isononyl isononanoate.

As a specific optimization scheme of this embodiment, the apple cell extract in step S5 is obtained by adding 75% ethanol into dry apple fruit cell culture powder according to a liquid-to-solid ratio of 1:2, pre-soaking for 15min, and performing ultrasonic extraction for 20min each time for 2 times at 25KHz and 300W.

As a specific optimization scheme of this embodiment, the vegetable oil and fat in step S3 is obtained by refining to remove various impurities and free fatty acids.

Example two: referring to fig. 1, the present invention discloses a method for preparing osmunda facial tightening eye cream containing apple cell extract, comprising the following steps:

s2, preparation of phase A: sequentially adding the thickening agent, the deionized water and the humectant in the step S1 into an emulsifying pot, stirring and heating to 82 ℃, dissolving completely, homogenizing properly for 1.5min, and keeping the temperature for later use;

s4, mixing of raw materials: adding the phase B in the step S3 into the phase A in the step S2, homogenizing for 3.5min, vacuumizing, and keeping the temperature and stirring for 17min for later use;

As a specific optimization scheme of this embodiment, the humectant in step S2 includes polyquaternium-511.5%, glycerin 2%, betaine 0.2%, allantoin 0.12%, 1, 3% butanediol, and sodium hyaluronate 0.015%, and the heat preservation time in step S2 is 8.5 min.

As a specific optimization scheme of this embodiment, the active extracts in step S3 include sargassum tonkinensis extract 0.8%, algae extract (nannochloropsis sp) 0.8%, inula japonica/leaf/stem extract 0.3%, and astragalus membranaceus root extract 0.8%.

As a specific optimization scheme of this embodiment, the effective lifting firming agent in step S3 includes divinyl dimethicone/dimethicone crosspolymer 1.9%, and hydroxypropyl cyclodextrin 1.2%.

As a specific optimization scheme of this embodiment, the antioxidant in step S5 includes 0.7% of fullerene, 0.3% of sodium benzotriazolyl styrene-butadiene-phenol sulfonate, 0.07% of pentaerythritol tetrakis (bis-tert-butyl hydroxyhydrocinnamate) and 0.4% of tocopherol (vitamin E).

Example three: referring to fig. 1, the present invention discloses a method for preparing osmunda facial tightening eye cream containing apple cell extract, comprising the following steps:

s2, preparation of phase A: sequentially adding the thickening agent, the deionized water and the humectant in the step S1 into an emulsifying pot, stirring and heating to 84 ℃, dissolving completely, homogenizing properly for 2min, and keeping the temperature for later use;

s4, mixing of raw materials: adding the phase B in the step S3 into the phase A in the step S2, homogenizing for 3-5min, vacuumizing, and keeping the temperature and stirring for 23min for later use;

As a specific optimization scheme of this embodiment, the humectant in step S2 includes-512% of polyquaternium, 4% of glycerol, 0.4% of betaine, 0.19% of allantoin, 2-5% of 1, 3-butanediol, and 0.025% of sodium hyaluronate, and the heat preservation time in step S2 is 9 min.

As a specific optimization scheme of this embodiment, the active extracts in step S3 include sargassum tonkinensis extract 1.3%, algae extract (nannochloropsis sp) 1.3%, inula japonica/leaf/stem extract 0.9%, and astragalus membranaceus root extract 1.8%.

As a specific optimization scheme of this embodiment, the effective lifting and tightening agent in step S3 includes divinyl dimethicone/dimethicone crosspolymer 2%, and hydroxypropyl cyclodextrin 1.5%.

As a specific optimization scheme of this embodiment, the antioxidant in step S5 includes 0.9% of fullerene, 0.25% of sodium benzotriazolyl styrene-butadiene-phenol sulfonate, 0.09% of pentaerythritol tetrakis (bis-tert-butyl hydroxyhydrocinnamate) and 0.5% of tocopherol (vitamin E).

Example four: referring to fig. 1, the present invention discloses a method for preparing osmunda facial tightening eye cream containing apple cell extract, comprising the following steps:

s2, preparation of phase A: sequentially adding the thickening agent, the deionized water and the humectant in the step S1 into an emulsifying pot, stirring and heating to 85 ℃, dissolving completely, homogenizing for 3min properly, and keeping the temperature for later use;

s4, mixing of raw materials: adding the phase B in the step S3 into the phase A in the step S2, homogenizing for 5min, vacuumizing, and keeping the temperature and stirring for 25min for later use;

As a specific optimization scheme of this embodiment, the humectant in step S2 includes polyquaternium-512.5%, glycerin 5%, betaine 0.5%, allantoin 0.2%, 1, 3-butanediol 2-5%, and sodium hyaluronate 0.03%, and the heat preservation time in step S2 is 10 min.

As a specific optimization scheme of this embodiment, the active extracts in step S3 include sargassum tonkinensis extract 1.5%, algae extract (nannochloropsis sp) 1.5%, inula japonica/leaf/stem extract 1%, and astragalus membranaceus root extract 2%.

As a specific optimization scheme of this embodiment, the effective lifting and tightening agent in step S3 includes 3% of divinyl dimethicone/dimethicone crosspolymer and 2% of hydroxypropyl cyclodextrin.

As a specific optimization scheme of this embodiment, the antioxidant in step S5 includes 1% of fullerene, 0.3% of sodium benzotriazolyl butylphenolate sulfonate, 0.1% of pentaerythritol tetrakis (bis-tert-butyl hydroxyhydrocinnamate) and 0.6% of tocopherol (vitamin E).

And (4) judging the standard: sensory evaluation

Selecting 30 subjects in each group, placing bean-grain eye cream around eyes in a spot-coating manner, massaging gently along the eye sockets, uniformly coating the eye cream on each part, and massaging gently in a clockwise looping manner to enable the eye cream to be completely absorbed by skin. Score after 2 minutes of standing.

Table 1: sensory evaluation table for each experimental group

According to evaluation feedback of a test subject in the table 1, the eye cream disclosed by the invention is moist but not greasy, easy to spread and quick to absorb, can immediately smooth fine wrinkles around eyes, realizes the effects of immediately tightening and removing wrinkles, and restores the eyes of bright eyes.

Evaluation of eye Care

Test objects: 500 volunteers were randomly selected, 40 human subjects with loose eye skin, obvious fine lines, puffy eyes and serious dark circles were selected for each experimental group, and were used 1 time each day in the morning and evening for 1 month continuously, the results are shown in table 2, and the effects are divided into three grades:

the eye nursing effect is obvious: the eye skin can be obviously felt to be tight, fine wrinkles are obviously reduced, puffiness around the eyes is obviously improved, and dark circles are obviously faded.

The eye care effect is general, the eye skin tightening effect is general, the fine wrinkle reducing effect is general, the eye edema improving effect is general, and the black eye fading effect is general.

Eye care effect was unchanged: the eye skin relaxation effect is not much different from the previous eye skin relaxation effect, the eye fine lines are obvious, the eye circumference is puffy, the dark eye circles are serious, and no obvious feeling is caused after the eye skin relaxation effect is used.

Table 2 evaluation of the use effect of each example and comparative example.

Skin elasticity efficacy verification

The products prepared in examples 1 to 4 and comparative examples 1 to 4 were tested for effectiveness on a trial basis, while a commercially available tightening eye cream was used as a control.

Test objects: each experimental group was 30 women (25-50 years old); test area: an eye portion; testing an instrument: skin elastometer (Cutometer MPA 580). Use of: 2 times daily for 4 weeks; and (3) testing items: skin elasticity.

The method comprises the following steps: the subjects were tested to take care of relaxing facial muscles and gently closing both eyes after sitting still in an environment of 24 ℃ at a relative humidity of 55% for 25min after cleansing the face on the day. The instant elasticity of the skin of the eyes of the eye is measured by an Elastimeter skin elasticity measuring instrument, the probe repeatedly measures the eye in the same measured area for 3 times during measurement, and the average value is taken. The force is uniform when the probe is used and the pressure applied should be constant. The subject again measures the elasticity values of the test area after 1 week, 2 weeks, 3 weeks, 4 weeks of use.

Test results and evaluation: test data analysis comparisons of elasticity before and after use of the product in the test area of the subject are shown in table 3:

the invention has the beneficial effects that: the invention can promote the regeneration of a large number of dormant stem cells of the skin, promote metabolism, enhance the elasticity and water-retaining property of the skin, reduce wrinkles, strengthen the activity of epidermal cells, help to improve spreadability and skin feel by adding the apple stem cells, enable the skin to be soft and smooth, and provide a hydrophobic film for the skin, the addition of the acetyl hexapeptide-8 can restore the activity of epidermal basal layer stem cells, maintain the renewal capability of the epidermis and improve the skin quality, supplements micromolecular collagen in the skin, can deeply care and tighten the skin, brings instant bright eyes and can also deeply repair the eye skin, smooth texture, light, silky, smooth and non-sticky feeling, easy spreading, high absorption speed, strong soft focus covering power, smooth wrinkles and invisible pores, effectively covers the wrinkles, can provide instant wrinkle-removing and tightening effects, and brighten the water bright eyes, and gives natural makeup with soft-burnt effect; the antioxidant and the active extract added in the invention supplement collagen for eye cream, repair fine lines and eye bags, tighten the whole eye contour, realize deep tightening, recover young spirit, effectively resist upper eyelid looseness and fishtail lines, enable double eyelids to be more obvious, further reduce swelling, scratch small fine lines, tighten big eyes, pay attention to moisture preservation, pay attention to antioxidation, activate skin cells, lighten dark circles, continuously nourish double eyes, enable skin around the eyes to be tender, prevent dry lines from appearing in advance, benefit each other in effect, relieve fatigue and stress, drive dark fine lines away, enable the skin to be like a soaked sponge, furthest improve absorption, endow skin elasticity again, and realize tight, smooth and wrinkled eye bright eye.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A preparation method of osmunda face tightening eye cream containing apple cell extract is characterized by comprising the following steps:

2. The method of claim 1, wherein the moisturizer of step S2 comprises polyquaternium-511-2.5%, glycerin 1-5%, betaine 0.1-0.5%, allantoin 0.1-0.2%, 1, 3-butylene glycol 2-5%, and sodium hyaluronate 0.01-0.03%, and the incubation time of step S2 is 8-10 min.

3. The method of claim 1, wherein the thickener in step S2 comprises two or more of xanthan gum, VP/hexadecene copolymer, scleroglucan, pullulanase polysaccharide, carbomer, and ammonium polyacryloyldimethyltaurate.

4. The method of claim 1, wherein the active extracts of step S3 include Isaria javanica extract 0.5-1.5%, algae extract (Nannochloropsis sp. extract) 0.5-1.5%, Clotrichia dioica/leaf/stem extract 0.1-1%, and Astragalus membranaceus root extract 0.5-2%.

5. The method of claim 1, wherein the emulsifier in step S3 comprises two or more of lecithin, cetearyl alcohol, glyceryl stearate, glyceryl oleate, cetyl alcohol, steareth-20, and polysorbate-40.

6. The method of claim 1, wherein the skin-feel conditioning agent of step S3 comprises two or more of silica, nylon-12, potassium cocoyl hydrolyzed oat protein, methyl gluceth-20, and glycerin polyacrylate.

7. The method of claim 1, wherein the immediate-effect firming agent in step S3 comprises 1-3% of divinyldimethicone/dimethicone crosspolymer and 1-2% of hydroxypropyl cyclodextrin.

8. The method of claim 1, wherein the vegetable oil or fat of step S3 comprises at least one of jojoba seed oil, safflower seed oil, sunflower seed oil, corn oil, sesame seed oil, macadamia ternifolia seed oil, olive oil, sunflower seed wax, and cercis negundo wax.

9. The method of claim 1, wherein the anti-oxidants of step S5 comprise fullerene 0.5-1%, sodium benzotriazolyl butylphenolate 0.1-0.3%, pentaerythritol tetrakis (bis-tert-butylhydroxyhydrocinnamate) 0.05-0.1%, tocopherol (vitamin E) 0.2-0.6%.

10. The method of claim 1, wherein the preservatives of step S5 comprise two or more of caprylyl glycol, phenoxyethanol, propylparaben, methylparaben, p-hydroxyacetophenone, and ethylhexylglycerin.

11. The method for preparing an osmunda face tightening eye cream containing an apple cell extract as claimed in claim 1, wherein the surfactant in the step S3 includes one or more of PEG-75 stearate, ceteth-20, and PEG-10 dimethicone.

12. The method of claim 1, wherein the emollient of step S3 comprises two or more selected from squalane, dimethicone, polyglyceryl-3, cyclopentadimethicone, polysiloxane-11, isohexadecane, caprylic/capric triglyceride, glycerol tris (ethylhexanoate), and isononyl isononanoate.

13. The method according to claim 1, wherein the apple cell extract in step S5 is obtained by pre-soaking dried apple fruit cell culture powder in 75% ethanol at a liquid-to-solid ratio of 1:2 for 15min, and ultrasonically extracting at 25KHz and 300W for 2 times, 20min each time.

14. The method of claim 1, wherein the vegetable oil and fat of step S3 is refined to remove impurities and free fatty acids.