CN113730289A - Eye and lip care composition with multiple repairing effects and application thereof - Google Patents
Eye and lip care composition with multiple repairing effects and application thereof Download PDFInfo
- Publication number
- CN113730289A CN113730289A CN202111043662.3A CN202111043662A CN113730289A CN 113730289 A CN113730289 A CN 113730289A CN 202111043662 A CN202111043662 A CN 202111043662A CN 113730289 A CN113730289 A CN 113730289A
- Authority
- CN
- China
- Prior art keywords
- solvent
- solution
- component
- oil
- polyquaternium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 50
- 230000000694 effects Effects 0.000 title claims abstract description 27
- 102000015636 Oligopeptides Human genes 0.000 claims abstract description 42
- 108010038807 Oligopeptides Proteins 0.000 claims abstract description 42
- 150000003384 small molecules Chemical class 0.000 claims abstract description 11
- 239000002904 solvent Substances 0.000 claims description 52
- 239000003921 oil Substances 0.000 claims description 32
- 235000019198 oils Nutrition 0.000 claims description 32
- 238000005303 weighing Methods 0.000 claims description 30
- 239000003813 safflower oil Substances 0.000 claims description 27
- 235000019485 Safflower oil Nutrition 0.000 claims description 25
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 25
- 235000005713 safflower oil Nutrition 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 24
- 241000130784 Arnebia euchroma Species 0.000 claims description 20
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 20
- 238000000855 fermentation Methods 0.000 claims description 19
- 230000004151 fermentation Effects 0.000 claims description 19
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 claims description 15
- 241001071917 Lithospermum Species 0.000 claims description 14
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 11
- OQILCOQZDHPEAZ-UHFFFAOYSA-N Palmitinsaeure-octylester Natural products CCCCCCCCCCCCCCCC(=O)OCCCCCCCC OQILCOQZDHPEAZ-UHFFFAOYSA-N 0.000 claims description 10
- GJQLBGWSDGMZKM-UHFFFAOYSA-N ethylhexyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC(CC)CCCCC GJQLBGWSDGMZKM-UHFFFAOYSA-N 0.000 claims description 10
- 229920006007 hydrogenated polyisobutylene Polymers 0.000 claims description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 10
- 238000010298 pulverizing process Methods 0.000 claims description 10
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 6
- 239000006185 dispersion Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 241000130781 Arnebia Species 0.000 claims description 5
- BYUQATUKPXLFLZ-UIOOFZCWSA-N CCCCCCCCCCCCCCCC(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(O)=O)CC1=CN=CN1 Chemical compound CCCCCCCCCCCCCCCC(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(O)=O)CC1=CN=CN1 BYUQATUKPXLFLZ-UIOOFZCWSA-N 0.000 claims description 5
- 244000020518 Carthamus tinctorius Species 0.000 claims description 5
- 235000003255 Carthamus tinctorius Nutrition 0.000 claims description 5
- 229940093441 palmitoyl oligopeptide Drugs 0.000 claims description 5
- XDOFQFKRPWOURC-UHFFFAOYSA-N 16-methylheptadecanoic acid Chemical compound CC(C)CCCCCCCCCCCCCCC(O)=O XDOFQFKRPWOURC-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 125000005456 glyceride group Chemical group 0.000 claims description 4
- 229940119170 jojoba wax Drugs 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 230000001502 supplementing effect Effects 0.000 claims description 3
- IHRKJQSLKLYWBQ-QKDODKLFSA-N (2s)-2-[[(2s)-1-[(2s)-5-amino-2-[[2-(hexadecanoylamino)acetyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-5-(diaminomethylideneamino)pentanoic acid Chemical compound CCCCCCCCCCCCCCCC(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O IHRKJQSLKLYWBQ-QKDODKLFSA-N 0.000 claims description 2
- WSGCRSMLXFHGRM-DEVHWETNSA-N (2s)-2-[[(2s)-6-amino-2-[[(2s,3r)-2-[[(2s,3r)-2-[[(2s)-6-amino-2-(hexadecanoylamino)hexanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxybutanoyl]amino]hexanoyl]amino]-3-hydroxypropanoic acid Chemical compound CCCCCCCCCCCCCCCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O WSGCRSMLXFHGRM-DEVHWETNSA-N 0.000 claims description 2
- 230000008901 benefit Effects 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims description 2
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 2
- 229940094946 palmitoyl tetrapeptide-7 Drugs 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000686 essence Substances 0.000 claims 1
- 239000000499 gel Substances 0.000 claims 1
- 230000003716 rejuvenation Effects 0.000 claims 1
- 230000004888 barrier function Effects 0.000 abstract description 4
- 150000002632 lipids Chemical class 0.000 abstract description 3
- 239000012466 permeate Substances 0.000 abstract description 2
- 239000013543 active substance Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000013329 compounding Methods 0.000 abstract 1
- 230000003750 conditioning effect Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 50
- 239000000243 solution Substances 0.000 description 43
- 210000004027 cell Anatomy 0.000 description 17
- 230000037303 wrinkles Effects 0.000 description 12
- 102000008186 Collagen Human genes 0.000 description 10
- 108010035532 Collagen Proteins 0.000 description 10
- 229920001436 collagen Polymers 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 7
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 229940106189 ceramide Drugs 0.000 description 7
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 7
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 7
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 6
- 102000012422 Collagen Type I Human genes 0.000 description 6
- 108010022452 Collagen Type I Proteins 0.000 description 6
- 229960002446 octanoic acid Drugs 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 210000001732 sebaceous gland Anatomy 0.000 description 3
- 210000000434 stratum corneum Anatomy 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 208000007287 cheilitis Diseases 0.000 description 2
- 210000004207 dermis Anatomy 0.000 description 2
- 239000008157 edible vegetable oil Substances 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000003906 humectant Substances 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 2
- 235000020778 linoleic acid Nutrition 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000693 micelle Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- NEZONWMXZKDMKF-JTQLQIEISA-N Alkannin Chemical compound C1=CC(O)=C2C(=O)C([C@@H](O)CC=C(C)C)=CC(=O)C2=C1O NEZONWMXZKDMKF-JTQLQIEISA-N 0.000 description 1
- 239000004229 Alkannin Substances 0.000 description 1
- APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- WLYGSPLCNKYESI-RSUQVHIMSA-N Carthamin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@]1(O)C(O)=C(C(=O)\C=C\C=2C=CC(O)=CC=2)C(=O)C(\C=C\2C([C@](O)([C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C(O)=C(C(=O)\C=C\C=3C=CC(O)=CC=3)C/2=O)=O)=C1O WLYGSPLCNKYESI-RSUQVHIMSA-N 0.000 description 1
- 241000208809 Carthamus Species 0.000 description 1
- 102400001244 Cerebellin Human genes 0.000 description 1
- 101800001299 Cerebellin Proteins 0.000 description 1
- 235000002673 Dioscorea communis Nutrition 0.000 description 1
- 241000544230 Dioscorea communis Species 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000035753 Periorbital contusion Diseases 0.000 description 1
- UNNKKUDWEASWDN-UHFFFAOYSA-N alkannin Natural products CC(=CCC(O)c1cc(O)c2C(=O)C=CC(=O)c2c1O)C UNNKKUDWEASWDN-UHFFFAOYSA-N 0.000 description 1
- 235000019232 alkannin Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 1
- 229960003942 amphotericin b Drugs 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000037365 barrier function of the epidermis Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000003570 cell viability assay Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 229940096422 collagen type i Drugs 0.000 description 1
- 238000013093 comparative effectiveness research Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000004177 elastic tissue Anatomy 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000001050 lubricating effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229950004354 phosphorylcholine Drugs 0.000 description 1
- -1 phosphorylcholine lipid Chemical class 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000005549 size reduction Methods 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8152—Homopolymers or copolymers of esters, e.g. (meth)acrylic acid esters; Compositions of derivatives of such polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
- A61Q1/02—Preparations containing skin colorants, e.g. pigments
- A61Q1/04—Preparations containing skin colorants, e.g. pigments for lips
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
- A61Q1/02—Preparations containing skin colorants, e.g. pigments
- A61Q1/04—Preparations containing skin colorants, e.g. pigments for lips
- A61Q1/06—Lipsticks
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/001—Preparations for care of the lips
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
Abstract
The invention provides an eye and lip care composition with multiple repairing efficacies and application thereof. The invention relates to an eye and lip care composition, which comprises the following components in percentage by weight: component b): component c) ═ 1-5: (1-10): (1-10), the component a) is a small molecule oligopeptide solution, the component b) is a skin conditioning agent, and the component c) is a polyquaternium-51 solution. By reasonable compounding and multi-target synergistic effect, the eye mask can form a breathable water-retaining film on the surface of skin, strengthen a lipid barrier, maintain the moisture of the eye lip skin, and simultaneously promote active substances to permeate to the deep layer, and has good repairing effect on the damaged, chapped and inflamed eye lip skin.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to an eye and lip care composition with multiple repairing efficacies and application thereof.
Background
The aged face is aged first, and the eyes are the places where the aging information is disclosed first. The eye skin is the thinnest part of human skin, the sum of the thicknesses of the epidermis and the dermis is about 0.55mm, is 1/3-1/5 of the thickness of the face skin, and is easily damaged by external stimulation; the eye part has no sweat gland and sebaceous gland under the skin basically, so that a water-fat film with the functions of locking water and lubricating the skin is difficult to form, and the thin skin around the eyes is easier to dry; eye support structures are rare, lack elastic fiber and collagen structures, and are more prone to fine lines and wrinkles; the eyes blink for 2.4-2.8 ten thousand times every day, which causes the eye muscle and the skin around the eye muscle to be stressed greatly, and easily causes the fatigue and the relaxation of the eyes.
Lip and eye skin are in fact similar and even more fragile than eye skin. The lips are the only mucosa tissues exposed outside of the whole body for a long time, only have 3-5 layers of keratinocytes, are extremely lack of melanin, and cannot resist the invasion of ultraviolet rays, so that the skin of the lips is dark and dull. The lips can not secrete grease by themselves because of no sebaceous glands, the protection of a sebaceous membrane is lacked, the water loss speed is 6 times of that of the facial skin, the skin of the lips is dry and skinned and cracked, and even cracks and cheilitis can be seriously caused. With age, collagen in the skin of the lips decreases and becomes less elastic, resulting in deeper and increased cheilogramma and even spreading beyond the lip line.
Common to the human eye and lip skin mechanisms are: the skin is thin, sebaceous glands are few, and the like, compared with the skin at other parts, the skin is more easily damaged by the external environment, lips are easy to dry and peel, crack, dark lip color and the like, and eyes are easy to have black eye circles, eye bags, fine lines or deep lines and the like.
At present, products on the market mostly focus on solving the problems of dryness, wrinkles, darkness and the like of the whole facial skin, products specially used for solving the problems of the lips are lacked, and the skin around the lips becomes the most sensitive and aging part compared with the skin in other areas, so that the development of a lip product which aims at the characteristics of the skin around the lips, has high safety, strong efficacy and strong pertinence is particularly important.
Shi Zi is originally recorded in Shen nong Ben Cao Jing (Shen nong's herbal), cold in nature, and has the actions of clearing heat and removing toxicity, cooling blood and stopping bleeding, promoting eruption and generating spots. Alkannin is one of the effective components of lithospermum, has pharmacological actions of resisting inflammation, resisting bacteria, promoting wound healing and the like, and has literature reports that the chronic cheilitis is treated by adopting self-made lithospermum wet-dressing liquid medicine, and the total effective rate of the treatment is 93.2%.
Safflower, a plant of the genus Carthamus, Compositae, also known as safflower: the red-blue flower and the thorn-safflower have special fragrance. Safflower seed oil extracted from dry ripe fruits of safflower is a healthy and precious high-quality edible oil, the main component of which is unsaturated fatty acid-linoleic acid which is necessary for human body but can not be synthesized in vivo by self, and the safflower seed oil is known as the king of linoleic acid with the highest content in the known plant edible oil. Linoleic acid is an important component of ceramide I, which plays a crucial role in extracellular lipid matrix formation, and ceramide I deficiency can lead to dry skin and scaling.
Oligopeptides, a class of polypeptides, are compounds formed by the condensation of 2-10 amino acids, generally having a molecular weight of less than 1000 daltons, also called small peptides, oligopeptides or called small molecule active peptides, which have a lower molecular weight than polypeptides and are more permeable to the skin and more easily absorbed by the skin of the human body. Such as palmitoyl tripeptide-1, as messenger peptide for collagen renewal, can stimulate fibroblasts to synthesize collagen and glycosaminoglycan, enhance the function of dermal structure, regulate the connective tissue reconstruction and cell proliferation processes, promote the generation of skin repair protein, and achieve the effects of repairing and removing wrinkles.
The polyquaternium-51 is used as a novel polymer humectant, has the moisturizing effect which is 2 times that of hyaluronic acid, can form a breathable water-retaining film on the surface of skin, and is used as a second water-retaining barrier of the skin barrier to keep the skin moist. The polyquaternium-51 is a phosphorylcholine lipid polymer, has components and characteristics similar to those of human cell membranes, can alleviate and inhibit the toxicity of irritants to cells, and promotes the self-repair of the cells. Meanwhile, the polyquaternium-51 can form nano micelle with an imitated cell membrane structure, so that the permeability of the effective components to the skin is enhanced, and deep and lasting skin care is achieved.
Disclosure of Invention
Aiming at the needs of the existing market, the invention aims to provide an eye-lip care composition with multiple repair efficacies and application thereof.
The purpose of the invention is realized by the following technical scheme:
in one aspect of the invention, an eye and lip care composition with multiple repairing efficacies is provided, wherein the composition comprises the following components in parts by weight: component b): component c) ═ 1-5: (1-10): (1-10);
the component a) is a small molecule oligopeptide solution, wherein the small molecule oligopeptide is selected from one or two combinations of oligopeptide-1, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4 and palmitoyl hexapeptide-8;
the component b) is a skin conditioner and is obtained by fermenting lithospermum oil and safflower oil, wherein the weight ratio of the lithospermum oil to the safflower oil is (1-5): (1-5);
the component c) is polyquaternium-51 solution.
Preferably, the weight ratio of the composition is preferably component a): component b): component c) 1: 5: 5.
preferably, the mass fraction of the total peptides in the component a) small molecule oligopeptide solution is 0.01-0.05%, and the rest is the first solvent; the first solvent is selected from one or two combination of polyglycerol-2 isostearate and hydrogenated polyisobutene.
More preferably, the arnebia oil of component b) is derived from dried roots extracted from arnebia euchroma (Royle) Johnst;
weighing an appropriate amount of dry roots of arnebia euchroma (Royle) Johnst, cutting into pieces of 1-2cm, adding a second solvent 4-8 times of arnebia euchroma (Royle) Johnst, soaking for 24h at 60-80 deg.C for 2-3h, filtering, and collecting filtrate to obtain arnebia euchroma (Royle) Johnst oil; the second solvent is one or the combination of at least two of ethylhexyl palmitate, caprylic/capric triglyceride and jojoba oil;
further preferably, the safflower oil is extracted from dried seeds of safflower;
weighing appropriate amount of fructus Carthami tinctorii, drying at 35-45 deg.C to constant weight, pre-pulverizing to about 30 mesh at-10 deg.C to-20 deg.C for 40-60 min; adding n-hexane solvent 6-10 times the weight of the powder, leaching at 65-85 deg.C for 0.5-1.5 hr, and concentrating under reduced pressure until the solvent is completely evaporated to obtain safflower oil.
Further preferably, the mass percent of the polyquaternium-51 in the polyquaternium-51 solution of the component c) is 1.5 to 3.0 percent, and the balance is a fourth solvent; wherein the fourth solvent is selected from one or at least two of caprylic/capric triglyceride, caprylic/capric glyceride and ethylhexyl palmitate.
In certain embodiments, the lip care compositions are prepared by the following method:
s1, weighing a proper amount of small molecular oligopeptide, adding a first solvent with the weight being 10-15 times of the weight of the small molecular oligopeptide for pre-dispersion, stirring at 40-50 ℃, uniformly mixing, and complementing the solvent to obtain a small molecular oligopeptide solution;
s2, weighing the lithospermum oil and the safflower oil according to the proportion, stirring and mixing uniformly, adding a fermentation strain, wherein the inoculation amount is 0.01-0.05% of the mixed oil, the inoculation temperature is 35-40 ℃, the fermentation time is 8-12h, filtering, and collecting filtrate, namely the skin conditioner;
s3, weighing polyquaternium-51, adding a fourth solvent, stirring and mixing uniformly, and then supplementing the fourth solvent to obtain the polyquaternium-51 solution;
s4, adding the small molecular oligopeptide solution and the skin conditioner solution into the polyquaternium-51 solution according to the proportion, heating to 65-70 ℃, mixing and stirring uniformly to obtain the eye and lip care composition with multiple repairing effects.
In certain more specific implementations, the micronizing device of S3 is a low-temperature vibratory pulverizer; the fermentation strain in S4 is yeast or lactobacillus.
The invention also provides a preparation method of the eye and lip care composition with multiple repairing efficacies, which comprises the following steps:
1) weighing a proper amount of small molecular oligopeptide, adding a first solvent with the weight being 10-15 times of the weight of the small molecular oligopeptide for pre-dispersion, stirring at 40-50 ℃, uniformly mixing, and then complementing the first solvent to obtain a small molecular oligopeptide solution; the mass fraction of the total peptides in the small molecule oligopeptide solution is 0.01-0.05%, and the balance is a first solvent; the first solvent is selected from one or two combination of polyglycerol-2 isostearate and hydrogenated polyisobutene.
2) Weighing an appropriate amount of dry roots of arnebia euchroma (Royle) Johnst, cutting into pieces of 1-2cm, adding a second solvent 4-8 times of arnebia euchroma (Royle) Johnst, soaking for 24h at 60-80 deg.C for 2-3h, filtering, and collecting filtrate to obtain arnebia euchroma (Royle) Johnst oil; the second solvent is one or the combination of at least two of ethylhexyl palmitate, caprylic/capric triglyceride and jojoba oil;
3) weighing appropriate amount of fructus Carthami tinctorii, drying at 35-45 deg.C to constant weight, pre-pulverizing to about 30 mesh with low temperature vibration pulverizer at-10 deg.C to-20 deg.C for 40-60 min; then adding n-hexane 6-10 times the weight of the powder, leaching at 65-85 deg.C for 0.5-1.5h, and concentrating under reduced pressure until n-hexane is completely evaporated to obtain safflower oil.
4) Weighing arnebia oil and safflower oil according to a certain proportion, stirring and mixing uniformly, adding a fermentation strain with the inoculation amount of 0.01-0.05% of the mixed oil, the inoculation temperature of 35-40 ℃, the fermentation time of 8-12h, filtering, and collecting filtrate to obtain the skin conditioner; the fermentation strain is yeast or lactobacillus.
5) Weighing polyquaternium-51, adding a fourth solvent, stirring and mixing uniformly, and then supplementing the fourth solvent to obtain the polyquaternium-51 solution; the mass percentage of the polyquaternium-51 in the polyquaternium-51 solution is 1.5-3.0%, and the balance is a fourth solvent; wherein the fourth solvent is selected from one or a combination of at least two of caprylic/capric triglyceride, caprylic/capric glyceride and ethylhexyl palmitate;
6) adding the small molecular oligopeptide solution and the skin conditioner solution into the polyquaternium-51 solution according to the proportion, heating to 65-70 ℃, mixing and stirring uniformly to obtain the eye and lip care composition with multiple repairing effects.
Preferably, the weight ratio of the lithospermum oil to the safflower oil in the step 4) is (1-5): (1-5); the weight ratio of the small molecular oligopeptide solution, the skin conditioner solution and the polyquaternium-51 solution in the step 6) is the component a): component b): component c) 1: 5: 5.
in certain embodiments, certain conditions may be further preferred:
1) weighing a proper amount of small molecular oligopeptide palmitoyl tripeptide-1, adding 10 times of hydrogenated polyisobutene for pre-dispersion, stirring at 40-50 ℃, uniformly mixing, and complementing the hydrogenated polyisobutene to obtain a small molecular oligopeptide solution, wherein the mass fraction of total peptides in the small molecular oligopeptide solution is 0.01-0.05%, and the balance is hydrogenated polyisobutene.
2) Weighing a proper amount of dry roots of arnebia euchroma, cutting into pieces of 1-2cm, adding ethylhexyl palmitate 6 times of arnebia euchroma, soaking for 24h at 65 deg.C for 2h, filtering, and collecting filtrate to obtain arnebia euchroma oil;
3) weighing appropriate amount of fructus Carthami tinctorii, drying at 35 deg.C to constant weight, pre-pulverizing to about 30 mesh with low temperature vibration pulverizer at-10 deg.C for 40 min; then adding n-hexane with the weight 8 times of the powder for leaching at 75 ℃ for 1h, and concentrating under reduced pressure until the n-hexane is completely evaporated to obtain safflower oil;
4) weighing the lithospermum oil and the safflower oil according to the proportion respectively, stirring and mixing uniformly, adding a fermentation strain, wherein the inoculation amount is 0.03 percent of the mixed oil, the inoculation temperature is 38 ℃, the fermentation time is 10 hours, filtering, and collecting filtrate to obtain the skin conditioner; the fermentation strain is lactobacillus.
5) Weighing polyquaternium-51, adding 40 times of solvent caprylic acid/capric acid triglyceride, stirring and mixing uniformly, and complementing the solvent caprylic acid/capric acid triglyceride to obtain the polyquaternium-51 solution; the mass percentage of the polyquaternium-51 in the polyquaternium-51 solution is 1.5-3.0%, and the balance is caprylic acid/capric acid triglyceride;
6) adding the small molecular oligopeptide solution and the skin conditioner solution into the polyquaternium-51 solution according to the proportion, heating to 65-70 ℃, mixing and stirring uniformly to obtain the eye and lip care composition with multiple repairing effects.
The weight ratio of the lithospermum oil to the safflower oil in the step 4) is (1-5): (1-5); the weight ratio of the small molecular oligopeptide solution, the skin conditioner solution and the polyquaternium-51 solution in the step 6) is 1: 5: 5.
in another aspect, the invention provides an application of the eye and lip care composition, wherein the application of the composition comprises but is not limited to lipstick, lip glaze, lipstick, lip dye, lip mud, lip mask, eye essence, eye gel and eye cream.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the safflower oil is prepared, and is subjected to fermentation pretreatment by adopting an ultramicro crushing technology, so that the cell wall breaking rate can be improved, and compared with the traditional extraction method, the safflower oil is more fully contacted with an extraction solvent, and is more favorable for dissolving and releasing active ingredients; according to the invention, the nano micelle structure characteristic of polyquaternium-51 is utilized to encapsulate the small molecular oligopeptide, the lithospermum oil and the safflower oil, so that the permeability of active ingredients can be improved, fat-soluble ingredients are helped to permeate through an epidermal barrier and reach a dermis layer, a targeting effect is exerted, and the anti-aging effect of the small molecular oligopeptide is enhanced.
Drawings
FIG. 1 is a graph showing the difference in ceramide-promoting effect between different compositions in Experimental example 1;
FIG. 2 is a graph showing the effect of different compositions on the type I collagen synthesis rate in Experimental example 2;
FIG. 3 is a graph showing the effect of the emulsion of Experimental example 3 containing the composition of example 7 on the moisture content of the skin around the eyes;
FIG. 4 is a graph of the effect of an emulsion containing the composition of example 7 on the overall reduction in size of canthus wrinkles in Experimental example 3;
FIG. 5 is a graph showing the effect of lip film containing the composition of example 7 on the average depth of wrinkles on the lips in Experimental example 4;
FIG. 6 is a graph showing the effect of lip film containing the composition of example 7 on the average cross-sectional area of lip wrinkles in Experimental example 4;
FIG. 7 is a graph showing the effect of improving lip gloss of a lip film containing the composition of example 7 in Experimental example 4.
Detailed Description
Embodiments of the present application will be described in detail by examples, so that how to apply technical means to solve technical problems and achieve technical effects of the present application can be fully understood and implemented.
The raw materials and equipment used in the present application are all common raw materials and equipment in the field, and are all from commercially available products, unless otherwise specified. The methods used in this application are conventional in the art unless otherwise indicated.
There are many other possible embodiments of the present invention, which are not listed here, and the embodiments claimed in the claims of the present invention can be implemented.
"comprising" or "including" is intended to mean that the compositions (e.g., media) and methods include the recited elements, but not excluding others. When used in defining compositions and methods, "consisting essentially of … …" is meant to exclude other elements having any significance to the combination of the stated objects. Thus, a composition consisting essentially of the elements defined herein does not exclude other materials or steps that do not materially affect the basic and novel characteristics of the claimed application. "consisting of … …" refers to trace elements and substantial process steps excluding other components. Embodiments defined by each of these transition terms are within the scope of the present application.
Examples
In the following examples and comparative examples of the present invention, a lip care composition having multiple repair functions was prepared by the following process:
1) weighing a proper amount of small molecular oligopeptide palmitoyl tripeptide-1, adding 10 times of hydrogenated polyisobutene for pre-dispersion, stirring at 40-50 ℃, uniformly mixing, and complementing the hydrogenated polyisobutene to obtain a small molecular oligopeptide solution, wherein the mass fraction of total peptides in the small molecular oligopeptide solution is 0.01-0.05%, and the balance is hydrogenated polyisobutene.
2) Weighing a proper amount of dry roots of arnebia euchroma, cutting into pieces of 1-2cm, adding ethylhexyl palmitate 6 times of arnebia euchroma, soaking for 24h at 65 deg.C for 2h, filtering, and collecting filtrate to obtain arnebia euchroma oil;
3) weighing appropriate amount of fructus Carthami tinctorii, drying at 35 deg.C to constant weight, pre-pulverizing to about 30 mesh with low temperature vibration pulverizer at-10 deg.C for 40 min; then adding n-hexane with the weight 8 times of the powder for leaching at 75 ℃ for 1h, and concentrating under reduced pressure until the n-hexane is completely evaporated to obtain safflower oil;
4) weighing the lithospermum oil and the safflower oil according to the proportion respectively, stirring and mixing uniformly, adding a fermentation strain, wherein the inoculation amount is 0.03 percent of the mixed oil, the inoculation temperature is 38 ℃, the fermentation time is 10 hours, filtering, and collecting filtrate to obtain the skin conditioner; the fermentation strain is lactobacillus.
5) Weighing polyquaternium-51, adding 40 times of solvent caprylic acid/capric acid triglyceride, stirring and mixing uniformly, and complementing the solvent caprylic acid/capric acid triglyceride to obtain the polyquaternium-51 solution; the mass percentage of the polyquaternium-51 in the polyquaternium-51 solution is 1.5-3.0%, and the balance is caprylic acid/capric acid triglyceride;
6) adding the small molecular oligopeptide solution and the skin conditioner solution into the polyquaternium-51 solution according to the proportion, heating to 65-70 ℃, mixing and stirring uniformly to obtain the eye and lip care composition with multiple repairing effects.
The weight ratio of the lithospermum oil to the safflower oil in the step 4) is (1-5): (1-5);
wherein said caprylic/capric triglyceride is a common solvent in the art and is directly commercially available.
Examples 1 to 9 and comparative examples 1 to 5 were prepared in the proportions shown in Table 1 by the above-described method, and tested for efficacy. Wherein the component a) is a micromolecular oligopeptide solution, the component b) is a skin conditioner solution, and the component c) is a polyquaternium-51 solution.
TABLE 1 compositions of examples and comparative examples
Experimental example 1 ceramide Synthesis-promoting test
The skin structure of lips is different from skin of other parts of the body, the water content and barrier function of Stratum Corneum (SC) are low, and Ceramide (CERs) are important lipids for maintaining SC function and are one of parameters for evaluating the barrier function of skin of lips.
HEKa cells of normal human skin, with Epilife containing 1% HKGS (human keratinocyte growth supplement)TMCulture medium (containing PSA solution: penicillin, streptomycin and amphotericin B) at 37 deg.C and 5% CO2Routinely in the incubator, when the cells have grown to 80% confluence, they are digested with Trypsin/EDTA (Trypsin) at about 2.5X 103Per cm2Density of (2) to 25cm2In the culture flask, passage 1 time every 3-5 d.
Taking cells in logarithmic growth phase for conventional digestion, and adjusting the concentration of HEKa cells to 5 x 104Inoculating 100 μ l/ml of the suspension into a 96-well plate, growing to a near-confluent state, and replacing the 96-well plate with EpilifeTMCulturing for 24h, adding 100 μ l sample (Epilife as diluent solvent) with mass fraction of 0.25%TMMedia). Negative control groups (cell and culture medium groups) are set, 5 multiple wells are set for each sample, the samples are placed in an incubator and acted for 24 hours, and supernatants of HEKa cells acted by different compositions are respectively collected and used for ELISA measurement.
The results are shown in FIG. 1, where:
1) the promoting effect of the compositions of examples 1 to 9 on ceramide is higher than that of comparative examples 1 to 3, which shows that the promoting effect of the ceramide is better after 3 single raw materials are combined in a certain proportion;
2) in the compositions of examples 1 to 9, the promoting effect on ceramide tends to rise along with the increase of the component a) small-molecule oligopeptide oil solution, the component b) skin conditioner oil solution and the component c) humectant, and the promoting effect of the component b) skin conditioner oil solution is particularly obvious and tends to be flat after reaching a certain amount.
3) Examples 7, 8 and 9 had the best ceramide-promoting effect.
4) Comparative example 4 the same components as in example 7, but the pulverization was carried out in the same manner as in ordinary pulverization, and it was found that the effect of the ultrafine pulverization was superior to that of the ordinary pulverization.
5) The comparative example 5 component lacks component c) as compared to example 7, the remaining process is the same, and it is found that the effect of example 7 is significantly better than that of comparative example 5, indicating that component c) has a significant positive effect on the composition of the invention.
EXAMPLE 2 fibroblast protein expression assay
Human skin fibroblasts (HDFs) are derived from the dermal layer of the skin and have significant functions in synthesizing collagen and elastin. The collagen synthesized by HDF cells is mainly type i collagen, and serves as the main component of collagen fibers.
HDF cells were supplemented with FM medium with 10% neonatal calf serum, penicillin (100IU/ml) and streptomycin (100. mu.g/ml), 37 ℃, 5% CO2Culturing under the condition. Then, the HDF cells in the logarithmic phase were cultured in suspension to adjust the cell density (the cell density was 111111/ml in the case of cell viability assay, and 90. mu.l/well was added to a 96-well plate; the cell density was 30000/ml in the case of collagen type I synthesis assay, and 2 ml/well was added to a 12-well plate), 37 ℃ with 5% CO2Culturing under the condition. After 24h, the cells were attached and 0.25% of the test sample (DMSO as the dilution solvent) and positive control (EGF, 10ng/ml) were added. The supernatants were harvested and tested for type i collagen content using an ELISA kit, with the cell number and the amount of collagen produced by untreated cells as references (═ 100%).
The results are shown in FIG. 2, where:
the synthesis rates of the types I collagen of the compositions in the examples 1 to 9 are all higher than those of the compositions in the comparative examples 1 to 3, which shows that the synthesis rates of the types I collagen can be improved by combining 3 single raw materials according to a certain proportion, wherein the promotion effect of the type I collagen synthesis rate of the example 7 is optimal, and the promotion effect of the ultra-micro grinding on the type I collagen synthesis rate is found to be better than that of the common grinding in the comparative examples 4 and 5 compared with the comparison of the example 7 respectively, and the composition containing the component c) has obvious positive promotion effect.
Based on this, considering the efficacy, cost and stability of the composition in combination, example 7 (component a): component b): component c) 1: 5: 5).
Experimental example 3
Fig. 3 is the skin moisture content around the eyes (A.U.).
Fig. 4 is an eye corner wrinkle overall size reduction ratio (%).
Experimental example 4
Screening of 35 healthy volunteers aged 30-45 years. Lip wrinkles were measured using 5% lip film of example 7 applied 1 time each day in the morning, noon and evening, 1 week and 2 weeks after product application using Antera 3D, and photographed using Janus II and C-CUBE, respectively, and the results were as follows:
FIG. 5 is the average lip wrinkle depth (mm);
FIG. 6 is the average cross-sectional area (mm) of the lip wrinkles2)；
FIG. 7 is a diagram showing the effect of improving lip wrinkles.
The details not described in the specification of the present application belong to the common general knowledge of those skilled in the art.
In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. "substantially" means within an acceptable error range, and a person skilled in the art can solve the technical problem within a certain error range to substantially achieve the technical effect.
It is also noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a good or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such good or system. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a commodity or system that includes the element.
The foregoing description shows and describes several preferred embodiments of the present application, but as aforementioned, it is to be understood that the application is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the application, which is to be protected by the claims appended hereto.
Claims (10)
1. An eye-lip care composition with multiple repairing efficacies, which is characterized by comprising the following components in percentage by weight: component b): component c) ═ 1-5: (1-10): (1-10);
the component a) is a small molecule oligopeptide solution, wherein the small molecule oligopeptide is selected from one or two combinations of oligopeptide-1, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4 and palmitoyl hexapeptide-8;
the component b) is a skin conditioner and is obtained by fermenting lithospermum oil and safflower oil, wherein the weight ratio of the lithospermum oil to the safflower oil is (1-5): (1-5);
the component c) is polyquaternium-51 solution.
2. The ophthalmic lip care composition according to claim 1, wherein the composition weight ratio, preferably component a): component b): component c) 1: 5: 5.
3. the composition according to claim 2, wherein the mass fraction of the total peptides in the component a) small molecule oligopeptide solution is 0.01-0.05%, and the first solvent is the rest; the first solvent is selected from one or two combination of polyglycerol-2 isostearate and hydrogenated polyisobutene.
4. The composition for caring lips, according to claim 2, wherein component b) the arnebia oil is derived from the dried root extracted from arnebia euchroma (sieb) sieb;
weighing an appropriate amount of dry roots of arnebia euchroma (Royle) Johnst, cutting into pieces of 1-2cm, adding a second solvent 4-8 times of arnebia euchroma (Royle) Johnst, soaking for 24h at 60-80 deg.C for 2-3h, filtering, and collecting filtrate to obtain arnebia euchroma (Royle) Johnst oil; the second solvent is one or the combination of at least two of ethylhexyl palmitate, caprylic/capric triglyceride and jojoba oil;
said safflower oil is extracted from dried seeds of safflower;
weighing appropriate amount of fructus Carthami tinctorii, drying at 35-45 deg.C to constant weight, pre-pulverizing to about 30 mesh at-10 deg.C to-20 deg.C for 40-60 min; adding n-hexane solvent 6-10 times the weight of the powder, leaching at 65-85 deg.C for 0.5-1.5 hr, and concentrating under reduced pressure until the solvent is completely evaporated to obtain safflower oil.
5. The composition for caring lips according to claim 2, wherein the polyquaternium-51 in the polyquaternium-51 solution of component c) is 1.5 to 3.0% by mass, and the fourth solvent is the rest; wherein the fourth solvent is selected from one or at least two of caprylic/capric triglyceride, caprylic/capric glyceride and ethylhexyl palmitate.
6. The composition according to any one of claims 1 to 5, which is obtained by a process comprising:
s1, weighing a proper amount of small molecular oligopeptide, adding a first solvent with the weight being 10-15 times of the weight of the small molecular oligopeptide for pre-dispersion, stirring at 40-50 ℃, uniformly mixing, and complementing the solvent to obtain a small molecular oligopeptide solution;
s2, weighing the lithospermum oil and the safflower oil according to the proportion, stirring and mixing uniformly, adding a fermentation strain, wherein the inoculation amount is 0.01-0.05% of the mixed oil, the inoculation temperature is 35-40 ℃, the fermentation time is 8-12h, filtering, and collecting filtrate, namely the skin conditioner;
s3, weighing polyquaternium-51, adding a fourth solvent, stirring and mixing uniformly, and complementing the solvent to obtain the polyquaternium-51 solution;
s4, adding the small molecular oligopeptide solution and the skin conditioner solution into the polyquaternium-51 solution according to the proportion, heating to 65-70 ℃, mixing and stirring uniformly to obtain the eye and lip care composition with multiple repairing effects.
7. The ophthalmic lip care composition according to claim 6,
the ultramicro crushing equipment in the S3 is a low-temperature vibration crusher;
the fermentation strain in S4 is yeast or lactobacillus.
8. A method of preparing an eye-lip care composition having multiple rejuvenation benefits comprising the steps of:
1) weighing a proper amount of small molecular oligopeptide, adding a first solvent with the weight being 10-15 times of the weight of the small molecular oligopeptide for pre-dispersion, stirring at 40-50 ℃, uniformly mixing, and then complementing the first solvent to obtain a small molecular oligopeptide solution; the mass fraction of the total peptides in the small molecule oligopeptide solution is 0.01-0.05%, and the balance is a first solvent; the first solvent is selected from one or two combinations of polyglycerol-2 isostearate and hydrogenated polyisobutene;
2) weighing an appropriate amount of dry roots of arnebia euchroma (Royle) Johnst, cutting into pieces of 1-2cm, adding a second solvent 4-8 times of arnebia euchroma (Royle) Johnst, soaking for 24h at 60-80 deg.C for 2-3h, filtering, and collecting filtrate to obtain arnebia euchroma (Royle) Johnst oil; the second solvent is one or the combination of at least two of ethylhexyl palmitate, caprylic/capric triglyceride and jojoba oil;
3) weighing appropriate amount of fructus Carthami tinctorii, drying at 35-45 deg.C to constant weight, pre-pulverizing to about 30 mesh with low temperature vibration pulverizer at-10 deg.C to-20 deg.C for 40-60 min; then adding n-hexane with the weight 6-10 times of the weight of the powder for leaching at 65-85 deg.C for 0.5-1.5h, and concentrating under reduced pressure until n-hexane is completely evaporated to obtain safflower oil;
4) weighing arnebia oil and safflower oil according to a certain proportion, stirring and mixing uniformly, adding a fermentation strain with the inoculation amount of 0.01-0.05% of the mixed oil, the inoculation temperature of 35-40 ℃, the fermentation time of 8-12h, filtering, and collecting filtrate to obtain the skin conditioner; the fermentation strain is yeast or lactobacillus;
5) weighing polyquaternium-51, adding a fourth solvent, stirring and mixing uniformly, and supplementing the fourth solvent to obtain the polyquaternium-51 solution; the mass percentage of the polyquaternium-51 in the polyquaternium-51 solution is 1.5-3.0%, and the balance is a fourth solvent; wherein the fourth solvent is selected from one or a combination of at least two of caprylic/capric triglyceride, caprylic/capric glyceride and ethylhexyl palmitate;
6) adding the small molecular oligopeptide solution and the skin conditioner solution into the polyquaternium-51 solution according to the proportion, heating to 65-70 ℃, mixing and stirring uniformly to obtain the eye and lip care composition with multiple repairing effects.
9. The preparation method according to claim 8, wherein the weight ratio of arnebia oil to safflower oil in step 4) is (1-5): (1-5);
the weight ratio of the small molecular oligopeptide solution, the skin conditioner solution and the polyquaternium-51 solution in the step 6) is the component a): component b): component c) 1: 5: 5.
10. use of the composition according to any one of claims 1 to 7 for the care of the lips, wherein the use of the composition includes, but is not limited to, lipsticks, lip glazes, lipsticks, lip stains, lip mud, lip masks, eye essences, eye gels, eye creams.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111043662.3A CN113730289B (en) | 2021-09-07 | 2021-09-07 | Eye and lip care composition with multiple repairing effects and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111043662.3A CN113730289B (en) | 2021-09-07 | 2021-09-07 | Eye and lip care composition with multiple repairing effects and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113730289A true CN113730289A (en) | 2021-12-03 |
| CN113730289B CN113730289B (en) | 2024-02-13 |
Family
ID=78736500
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111043662.3A Active CN113730289B (en) | 2021-09-07 | 2021-09-07 | Eye and lip care composition with multiple repairing effects and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113730289B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115400067A (en) * | 2022-09-30 | 2022-11-29 | 上海臻臣化妆品有限公司 | Skin whitening composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112587472A (en) * | 2020-12-19 | 2021-04-02 | 广州市拉凯尔干细胞研究所 | Preparation method of osmunda japonica and face tightening eye cream containing apple cell extract |
-
2021
- 2021-09-07 CN CN202111043662.3A patent/CN113730289B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112587472A (en) * | 2020-12-19 | 2021-04-02 | 广州市拉凯尔干细胞研究所 | Preparation method of osmunda japonica and face tightening eye cream containing apple cell extract |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115400067A (en) * | 2022-09-30 | 2022-11-29 | 上海臻臣化妆品有限公司 | Skin whitening composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113730289B (en) | 2024-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6620419B1 (en) | Cosmetic or dermopharmaceutical use of peptides for healing, hydrating and improving skin appearance during natural or induced ageing (heliodermia, pollution) | |
| JP4099173B2 (en) | Cosmetic or dermopharmaceutical composition comprising kombucha | |
| JP6587678B2 (en) | Camellia japonica extract and cosmetic composition containing the same | |
| CN103619415A (en) | Exopolysaccharide for the treatment and/or care of the skin, mucous membranes, hair and/or nails | |
| CN110013447B (en) | Cream for removing striae gravidarum and preparation method thereof | |
| CN114224768A (en) | Repairing and anti-aging composition and preparation method thereof | |
| CN115137665A (en) | Composition with long-acting moisturizing and relieving effects and application thereof | |
| KR102054793B1 (en) | Cosmetic composition Cosmetic for improving skin elasticity and reducing dark circles containing natural extract and fermented extract | |
| CN113730289B (en) | Eye and lip care composition with multiple repairing effects and application thereof | |
| CN111388394B (en) | Biphase freeze-dried powder solvent and preparation method and application thereof | |
| KR100858449B1 (en) | Cosmetic compositions comprising extract of viola tricolor l. stabilized in nano-liposome | |
| KR102130243B1 (en) | A cosmetic composition for skin whitening, anti-wrinkle, anti-aging, and anti-oxidation containing glutathione, natural spicule powder, acetyl hexapeptide and broussonetia kazinoki extract | |
| KR102129430B1 (en) | Cosmetic composition comprising functional peptides and fermented products | |
| KR101953676B1 (en) | Cosmetic composition for skin moisturizing containing ultrasonicating extract of harpagophytum procumbens | |
| KR101843976B1 (en) | Cosmetic composition comprising extract of geminated phaseolus radiatus fermented by aureobasidium pullulans | |
| KR102424510B1 (en) | cosmetic composition for antiirritation and skin soothing using Lactobacillus rhamnosus J2K-187strain | |
| KR102162377B1 (en) | Extracts derived from natural products and cosmetic composition containing the same, and process for producing the same | |
| CN114712285A (en) | Composition with anti-wrinkle effect and preparation method and application thereof | |
| KR100558751B1 (en) | Cosmetic composition for skin miosture | |
| KR100490212B1 (en) | Anti-aging cosmetics composition containing amino acid complex | |
| KR101997231B1 (en) | Cosmetic composition containing enzyme-treated honey extract | |
| WO2022003056A1 (en) | Extract of hibiscus sabdariffa and use thereof for improving the barrier function and promoting the moisturization and desquamation of the skin | |
| KR100434898B1 (en) | Cosmetic composition containing medical plant complex extracts stabilized with liquid crystalsome | |
| CN111265451A (en) | Protein rejuvenation and repairing freeze-dried mask and preparation method thereof | |
| KR101448993B1 (en) | Pharmaceutical agent containing the Betula platyphylla Sukatchev var. japonica Hara extract as an effective ingredient and its use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |