CN111265451A

CN111265451A - Protein rejuvenation and repairing freeze-dried mask and preparation method thereof

Info

Publication number: CN111265451A
Application number: CN202010249980.4A
Authority: CN
Inventors: 刘婷; 王玥; 潘仙华; 谢婷庭; 杨艳芳; 施雁勤; 周亮; 李学娇
Original assignee: Shanghai Institute of Technology
Current assignee: Shanghai Institute of Technology
Priority date: 2020-04-01
Filing date: 2020-04-01
Publication date: 2020-06-12

Abstract

The invention discloses a protein rejuvenating and repairing freeze-dried mask and a preparation method thereof, wherein a protein composition, shea butter, a tetrandra root extract, β -sitosterol, lactose, a centella asiatica extract, sorbitan sesquioleate, sorbitan olivate, 1, 2-hexanediol, allantoin, EDTA disodium, xanthan gum, glycerol and deionized water are used as raw materials, all the raw materials are prepared into effective components, and the effective components are attached to a mask cloth and then freeze-dried.

Description

Protein rejuvenation and repairing freeze-dried mask and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, in particular to a protein rejuvenation and repairing freeze-dried mask and a preparation method thereof.

Background

The skin plays a barrier role in protecting the human body, and it protects various tissues and organs in the body from physical, chemical and pathogenic microorganisms. However, with the aging and the influence of the external environment, the skin aging degree gradually increases, which not only affects the beauty, but also gradually declines the ability to resist the external irritation, so the skin care is very important.

The general facial mask is usually manufactured in a wet manner, each sheet contains 20-30ml of water and is compounded with other various components, and the preservative therein can ensure that the facial mask does not deteriorate for a long time. In a liquid environment, the activity of some ingredients is greatly reduced, and some ingredients even cause sensitization reaction to skin. The non-woven fabric or the elastic facial mask paper is soaked in the essence with the beauty components, the facial mask becomes a small portable package after being sealed and packaged, when the facial mask is used, the sealed package of the facial mask is torn open, and the facial mask which is full of the essence can be directly taken out and applied to the face, so that the effects of beauty and maintenance are achieved. However, the wet mask has the following disadvantages in general: (1) the skin absorbs the nutrient components and simultaneously absorbs the preservative; (2) the mask cloth and the mask bag are soaked in the mask liquid for a long time, and the leaching of some harmful substances can also influence the health of the skin; (3) some unstable but highly bioactive materials will decay in the mask, which is not conducive to preservation and performance of their biological effects.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the existing mask has the problems of insufficient rejuvenation and repairing effect, easy deterioration, instability and easy sensitization.

The technical problem to be solved by the invention is realized by the following technical scheme:

the freeze-dried mask for rejuvenating and repairing protein is characterized by comprising, by weight, 1-2 parts of a protein composition, 0.3-0.8 part of shea butter, 0.3-0.8 part of radix stephaniae tetrandrae extract, 0.3-0.8 part of β -sitosterol, 0.1-0.5 part of lactose, 0.5-1.5 parts of centella extract, 0.1-0.3 part of sorbitan sesquioleate, 0.3-0.5 part of sorbitan oliveate, 0.3-0.8 part of 1, 2-hexanediol, 0.03-0.08 part of allantoin, 0.01-0.05 part of EDTA disodium, 0.1-0.5 part of xanthan gum, 3-8 parts of glycerol and 85-90 parts of deionized water.

Preferably, the raw materials comprise, by weight, 1-1.5 parts of a protein composition, 0.3-0.5 part of shea butter, 0.3-0.5 part of tetrandra root extract, 0.3-0.5 part of β -sitosterol, 0.1-0.3 part of lactose, 0.8-1.2 parts of centella asiatica extract, 0.1-0.2 part of sorbitan sesquioleate, 0.3-0.4 part of sorbitan olivetoleate, 0.5-0.7 part of 1, 2-hexanediol, 0.04-0.06 part of allantoin, 0.04-0.05 part of disodium EDTA, 0.1-0.3 part of xanthan gum, 4-6 parts of glycerol and 88-90 parts of deionized water.

More preferably, the raw material comprises 1.3 parts of protein composition, 0.5 part of shea butter, 0.5 part of tetrandra root extract, β -sitosterol, 0.25 part of lactose, 1 part of centella asiatica extract, 0.2 part of sorbitan sesquioleate, 0.4 part of sorbitan olivate, 0.6 part of 1, 2-hexanediol, 0.05 part of allantoin, 0.05 part of EDTA disodium, 0.2 part of xanthan gum, 5 parts of glycerol and 89.35 parts of deionized water in parts by weight.

Preferably, the protein composition comprises one or more of milk protein, whey protein, lactoferrin, and collagen.

More preferably, the protein composition consists of milk protein, whey protein, lactoferrin and collagen in a mass ratio of (4-6): 8-12): 1-2): 8-12.

Further, the protein composition is composed of milk protein, whey protein, lactoferrin and collagen in a mass ratio of 5:10:1: 10.

The invention also provides a preparation method of the protein rejuvenation and repairing freeze-dried mask, which is characterized in that all raw materials are prepared into functional components, and the functional components are attached to a mask cloth and then freeze-dried.

Preferably, the method comprises the following steps of (1) mixing glycerol, shea butter, radix stephaniae tetrandrae extract, β -sitosterol, lactose, centella asiatica extract, sorbitan sesquioleate, sorbitan olivetoleate, 1, 2-hexanediol, allantoin, xanthan gum and deionized water according to the formula, heating to 60 ℃, stirring to completely dissolve the mixture, then cooling to 40 ℃, adding a protein composition and EDTA disodium, uniformly mixing, dispersing until no obvious particles exist to prepare a mask liquid, storing the mask liquid in a sterilized container, (2) spraying the mask liquid on the surface of a mask cloth, circularly and uniformly spraying for multiple times to prepare a primary semi-finished product, and (3) freeze-drying, dehumidifying and packaging the sprayed semi-finished product at-200 to-40 ℃ to obtain the protein rejuvenation freeze-dried mask.

Preferably, when the protein rejuvenation and repairing freeze-dried mask is used, the protein rejuvenation and repairing freeze-dried mask can be used after being wetted by clear water after opening a bag.

The invention provides a preservative-free face-refreshing and repairing freeze-dried mask, which is characterized in that the face-beautifying components are uniformly coated on a mask paper layer and kept dry, and the freeze-dried mask is more beneficial to the storage of effective active components in the mask than the traditional wet mask. The active ingredients in the membrane are instantly frozen to be solid due to the freeze-drying technology, so that the active ingredients are easy to store, the probability of bacterial breeding is reduced, the use of a preservative is avoided, and the sanitation and safety of users are guaranteed.

Functional components such as a protein composition and a centella asiatica extract are added into the freeze-dried mask, so that the freeze-dried mask has multiple functions, and the functions of the components in the mask are as follows:

lactoferrin: lactoferrin exists in milk, tears, blood and the like, is an iron-binding glycoprotein sugar, has a molecular weight of about 8 ten thousand, and lactoferrin and a protein degradation product thereof, namely lactoferrin peptide, have wide biological activities, including broad-spectrum antibacterial action, inflammation diminishing, tumor cell growth inhibition, organism immune reaction regulation and the like, are considered to be novel antibacterial and anticancer drugs, and food and cosmetic additives with great development potential.

Whey protein: can reactivate skin cells and initiate synthesis of extracellular matrix molecules in the dermis, thereby reducing the apparent signs of aging. The in vivo test shows that the skin-care product can tighten the skin, enhance the elasticity, quickly and efficiently reduce the wrinkle depth and improve the skin.

Milk protein: is a general term of a plurality of protein mixtures in milk, has multiple effects of nourishing and moisturizing, whitening and activating skin, resisting aging, resisting inflammation, repairing and inhibiting bacteria, is widely applied to cosmetics, and endows the products with various effects.

Collagen protein: with the increase of age, the synthesis capacity of fibroblasts is reduced, if the skin lacks collagen, collagen fibers are solidified, intercellular mucopolysaccharide is reduced, the skin loses softness, elasticity and luster, and is aged, meanwhile, fiber breakage, lipoatrophy, sweat gland and sebaceous gland secretion of dermis are reduced, and a series of aging phenomena such as color spots, wrinkles and the like appear on the skin. When the active substance is used in cosmetics, the tyrosine contained in the active substance can diffuse to the deep layer of the skin, compete with tyrosine in the skin and combine with a catalytic center of tyrosinase, so that the generation of melanin is inhibited, the activity of collagen in the skin is enhanced, the integrity of stratum corneum moisture and a fiber structure is maintained, the metabolism of skin tissues is promoted, and good moistening, moisturizing and wrinkle-removing cosmetic effects are generated on the skin.

Centella asiatica extract: asiaticoside is a hydrophilic sugar chain, mainly glucose and rhamnose, can be combined with water in occlusion layer to improve epidermal barrier ability, enhance skin moisture, and remarkably increase hydration degree of stratum corneum; terpenoids in centella asiatica extract can significantly increase the content of collagen and cell layer fibronectin (fibronectin is widely involved in cell migration, adhesion, proliferation, hemostasis, skin tissue repair, etc.), and can stimulate the production of type I collagen which promotes scar maturation and reduce the generation of inflammatory response. In addition, the centella asiatica extract can reduce the production of proinflammatory mediators (IL-1, MMP-1), and improve and repair the barrier function of the skin.

According to the protein rejuvenation and repairing freeze-dried mask, the protein composition is added, other raw materials are reasonably proportioned, the low-temperature freeze-drying technology is utilized, and the production conditions are reasonably controlled, so that the prepared freeze-dried mask is easy to store and not easy to deteriorate, and has the effects of remarkably repairing and repairing skin, enabling the skin to be tender and elastic, and rejuvenating and brightening the skin after being used; meanwhile, the protein rejuvenation and repairing freeze-dried mask prepared by the invention has the characteristics of no addition of preservative, essence and pigment, good safety, no extract, capability of keeping high biological activity after being opened and ensuring that the skin can fully absorb nutrient substances, and the like.

Detailed Description

In order to make the invention more comprehensible, preferred embodiments are described in detail below.

Example 1

A preparation method of a protein rejuvenation and repairing freeze-dried mask comprises the following steps:

first, formula

The skin care cream comprises the following raw materials, by weight, 1.3 parts of a protein composition, 0.5 part of shea butter, 0.5 part of a tetrandra root extract, β -sitosterol, 0.25 part of lactose, 1 part of a centella asiatica extract, 0.2 part of sorbitan sesquioleate, 0.4 part of sorbitan olivate oleate, 0.6 part of 1, 2-hexanediol, 0.05 part of allantoin, 0.05 part of EDTA disodium, 0.2 part of xanthan gum, 5 parts of glycerol and 89.35 parts of deionized water.

The protein composition is composed of milk protein, whey protein, lactoferrin and collagen in a mass ratio of 5:10:1: 10.

Second, preparation method

(1) Preparing materials according to formula, such as glycerol, shea butter, radix Stephaniae Japonicae extract, β -sitosterol, lactose, herba Centellae extract, sorbitan sesquioleate, sorbitan olivate oleate, 1, 2-hexanediol, and allantoin, and weighing for use;

(2) mixing deionized water, lactose and glycerol, heating to 60 deg.C, maintaining the temperature for 60min, and stirring at 2000r/min to completely dissolve to obtain mixture A;

(3) mixing shea butter, radix Stephaniae Japonicae extract, β -sitosterol, herba Centellae extract, sorbitan sesquioleate, sorbitan olivate oleate, 1, 2-hexanediol, allantoin and xanthan gum, and stirring to obtain mixture B;

(4) cooling the mixture A to 40 ℃, adding the protein composition and EDTA disodium, uniformly mixing with the mixture B, stirring at 2000r/min for 1h, and dispersing until no obvious particles exist to obtain a mixed mask liquid;

(5) inspecting the mixed facial mask liquid, and storing the qualified mixed facial mask liquid in a sterilized container;

(6) spraying the uniformly mixed mask liquid on the surface of mask cloth, circularly spraying for multiple times until the mask liquid is uniform, and fully mixing to prepare a semi-finished product;

(7) freeze drying, vacuum-pumping the sprayed semi-finished product at-40 deg.C, freeze drying, and packaging.

(8) Each product is packaged under vacuum. The final finished product is the protein rejuvenation and repairing freeze-dried mask.

Comparative example 1

The skin care mask of this comparative example was substantially the same as the skin care mask of example 1 except that the skin care composition was different, and the skin care composition of this comparative example did not contain lactoferrin.

The preparation method of the skin care mask of the comparative example comprises the following steps: the same as in example 1.

Comparative example 2

The skin care mask of the comparative example is basically the same as the skin care mask of example 1, except that the preparation method of the mask of the comparative example does not adopt a freeze-drying technology, but directly soaks the mask cloth in the mask liquid for bagging.

And (3) performance testing:

firstly, human body safety test: patch test (patch test), the test substance is the protein rejuvenation freeze-dried mask, i.e. example 1.

The test method comprises the following steps:

test materials and instruments-Spot tester, Filter paper sheet (FINN CHAMBER), pipette gun (VITLAB, Germany)

The testing process comprises the following steps: according to a spot-patch test method of technical specification 2015 edition of cosmetic safety, 30 persons meeting the standard are selected for carrying out skin-closed spot-patch test.

(1) Negative control: a blank control was selected.

(2) Subject: age 18-40 years, 11 men, 19 women. Cleaning the skin of the back of the subject, and stabilizing for 30 min;

(3) the selected area is not more than 50mm²And qualified spot test equipment with the depth of about 1 mm. After the mask was moistened by water, a 40mm2 mask piece and a control group were randomly placed in a plaque tester chamber. The sample size selection was performed according to the relevant requirements in the cosmetic safety specifications 2015 edition.

(4) The spot test device with the test object is applied to the back of the subject with hypoallergenic tape, and is applied to the skin evenly by pressing with the palm for 24 h. The skin reactions were observed according to the standard of table 1 30min (after disappearance of the indentation), 24h and 48h after removal of the test article plaque test device, respectively, and the observation results were recorded.

TABLE 1 skin closed Patch test skin response grading Standard

The test results show that no adverse skin reactions occurred in 30 people.

Negative control: no adverse skin reactions occurred in 30 people.

TABLE 2 closed test results of skin patches for human body

II, testing the effect of the finished product:

30 women aged 18-40 years old were selected as volunteers, and were randomly divided into A, B, C groups of 10 people each, wherein group A used the protein rejuvenating and repairing lyophilized mask described in example 1 of the present invention, group B used the mask described in comparative example 1, and group C used the mask described in comparative example 2 every two days.

Test 1: in the moisturizing effect test, the test substances are the protein rejuvenation repairing freeze-dried mask described in example 1, the mask described in comparative example 1, and the mask described in comparative example 2.

Testing an instrument: corneometer CM825

The experimental environment is as follows: 22 +/-2 ℃, humidity of 50 +/-5 percent and real-time dynamic monitoring.

The test method comprises the following steps: selecting 3 test points, namely the forehead and the two cheeks, measuring the skin in the same area as much as possible when testing the group each time, standing for 30 minutes in a test environment without drinking water 30 minutes before testing, and then starting testing. The water content of the product used for 7 days, 14 days and 28 days was measured 3 times per area with the test result before the product was not used as a blank control (day 0), the measured values of each person in each area were counted, and the values of all the measured areas of each group of persons were collected and averaged. The results are shown in Table 3.

TABLE 3 skin moisture Change values

Test results as can be seen from the experiments of the moisturizing test in table 3, the skin care mask of group a had a strong moisturizing effect, and the moisturizing effect of the skin care mask of group B, C was inferior to that of group a.

And (3) testing 2: skin brightness test, the test substances are a protein rejuvenation repairing freeze-dried facial mask described in example 1, a facial mask described in comparative example 1, and a facial mask described in comparative example 2.

Testing an instrument: VISIA-CR

The test method comprises the following steps: and (3) measuring the skin in the same area as much as possible during each test, standing for 30 minutes in a test environment without drinking water 30 minutes before the test, and then starting the test. Detecting the same part of the subject by using VISIA at the 0 th day and the 28 th day; the test part was the whole face, the face was photographed, and analyzed by professional image analysis software, and the skin brightness of the face was measured (skin brightness was measured 3 times, and the average value thereof was taken as skin brightness reference data), whereby the skin brightness change rate (skin brightness change rate — skin brightness after test)/skin brightness before test) was calculated. The brighter the skin the higher the value, and the darker the skin the lower the value. Specific results are shown in table 4.

TABLE 4 skin Brightness variation values

And (3) testing results: as can be seen from the data in table 4, the best technical solution is obtained in example 1, and the measured skin brightness effect is the best; in example 1, it can be seen that the effect of improving skin brightness of the prepared mask is reduced if the operating parameters are different from those of example 1, as compared with comparative examples 1 and 2.

The above experimental results prove that: the freeze-drying technology is beneficial to the preservation of effective active ingredients, so that the effective active ingredients can be better exerted; the addition of the lactoferrin can obviously improve the efficacy of the facial mask, and has the effects of obviously improving the skin brightness, tightening the skin, making the skin tender and elastic, and refreshing the skin to be youthful and bright.

Claims

1. The freeze-dried mask for rejuvenating and repairing protein is characterized by comprising, by weight, 1-2 parts of a protein composition, 0.3-0.8 part of shea butter, 0.3-0.8 part of radix stephaniae tetrandrae extract, 0.3-0.8 part of β -sitosterol, 0.1-0.5 part of lactose, 0.5-1.5 parts of centella extract, 0.1-0.3 part of sorbitan sesquioleate, 0.3-0.5 part of sorbitan oliveate, 0.3-0.8 part of 1, 2-hexanediol, 0.03-0.08 part of allantoin, 0.01-0.05 part of EDTA disodium, 0.1-0.5 part of xanthan gum, 3-8 parts of glycerol and 85-90 parts of deionized water.

2. The freeze-dried mask for rejuvenating and repairing protein as set forth in claim 1, wherein the raw materials comprise, by weight, 1 to 1.5 parts of protein composition, 0.3 to 0.5 part of shea butter, 0.3 to 0.5 part of tetrandra root extract, 0.3 to 0.5 part of β -sitosterol, 0.1 to 0.3 part of lactose, 0.8 to 1.2 parts of centella asiatica extract, 0.1 to 0.2 part of sorbitan sesquioleate, 0.3 to 0.4 part of sorbitan olivate, 0.5 to 0.7 part of 1, 2-hexanediol, 0.04 to 0.06 part of allantoin, 0.04 to 0.05 part of disodium EDTA, 0.1 to 0.3 part of xanthan gum, 4 to 6 parts of glycerol, and 88 to 90 parts of deionized water.

3. The protein rejuvenation freeze-dried mask as set forth in claim 2, wherein the raw materials comprise, by weight, 1.3 parts of the protein composition, 0.5 parts of shea butter, 0.5 parts of tetrandra root extract, 0.5 parts of β -sitosterol, 0.25 parts of lactose, 1 part of centella asiatica extract, 0.2 parts of sorbitan sesquioleate, 0.4 parts of sorbitan olivo oleate, 0.6 parts of 1, 2-hexanediol, 0.05 parts of allantoin, 0.05 parts of disodium EDTA, 0.2 parts of xanthan gum, 5 parts of glycerin, and 89.35 parts of deionized water.

4. The protein rejuvenation freeze-dried mask as claimed in any one of claims 1 to 3, wherein said protein composition comprises one or more of milk protein, whey protein, lactoferrin and collagen.

5. The freeze-dried protein rejuvenating mask as set forth in claim 4, wherein the protein composition comprises milk protein, whey protein, lactoferrin and collagen in a mass ratio of (4-6) to (8-12) to (1-2) to (8-12).

6. The protein rejuvenation freeze-dried mask as claimed in claim 5, wherein the protein composition is composed of milk protein, whey protein, lactoferrin and collagen in a mass ratio of 5:10:1: 10.

7. The method for preparing the protein rejuvenating freeze-dried mask as defined in any one of claims 1 to 6, wherein all the raw materials are prepared into functional components, and the functional components are attached to a mask cloth and then freeze-dried.

8. The preparation method of the lyophilized facial mask for rejuvenating and repairing protein according to claim 7, wherein the method comprises the steps of (1) mixing glycerol, shea butter, Stephania tetrandra extract, β -sitosterol, lactose, centella asiatica extract, sorbitan sesquioleate, sorbitan olivate, 1, 2-hexanediol, allantoin, xanthan gum and deionized water in formula amounts, heating to 60 ℃, stirring to completely dissolve, cooling to 40 ℃, adding the protein composition and disodium EDTA, mixing uniformly, dispersing until no obvious particles are formed into a mask solution, storing in a sterilized container, (2) spraying the mask solution on the surface of a mask cloth, circulating for multiple times to spray uniformly to form a primary semi-finished product, and (3) lyophilizing, dehumidifying and packaging the sprayed semi-finished product at-200 ℃ to-40 ℃ to obtain the lyophilized facial mask for rejuvenating and repairing protein.

9. The method for preparing the protein rejuvenating freeze-dried mask as defined in claim 7 or 8, wherein the protein rejuvenating freeze-dried mask is used after being wetted with clear water after opening a bag.