CN112384233A - 一种从党参边角料中制备党参提取物的方法 - Google Patents
一种从党参边角料中制备党参提取物的方法 Download PDFInfo
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- CN112384233A CN112384233A CN201980042432.XA CN201980042432A CN112384233A CN 112384233 A CN112384233 A CN 112384233A CN 201980042432 A CN201980042432 A CN 201980042432A CN 112384233 A CN112384233 A CN 112384233A
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- codonopsis pilosula
- extract
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- 239000000284 extract Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000756943 Codonopsis Species 0.000 title abstract description 11
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract description 23
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract description 23
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- 108010059892 Cellulase Proteins 0.000 claims abstract description 18
- 229940106157 cellulase Drugs 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002904 solvent Substances 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 241000007126 Codonopsis pilosula Species 0.000 claims description 68
- 239000000463 material Substances 0.000 claims description 52
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 229940088598 enzyme Drugs 0.000 claims description 10
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
- 230000035484 reaction time Effects 0.000 claims description 4
- 238000000638 solvent extraction Methods 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 235000021190 leftovers Nutrition 0.000 description 38
- 239000003814 drug Substances 0.000 description 8
- 238000000605 extraction Methods 0.000 description 7
- 239000002699 waste material Substances 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 6
- 229920001282 polysaccharide Polymers 0.000 description 6
- 239000005017 polysaccharide Substances 0.000 description 6
- 229930182490 saponin Natural products 0.000 description 6
- 150000007949 saponins Chemical class 0.000 description 6
- 235000017709 saponins Nutrition 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
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- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 206010038084 Rectocele Diseases 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
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- 238000000855 fermentation Methods 0.000 description 1
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- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
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- 230000002195 synergetic effect Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/20—Preparation of steroids containing heterocyclic rings
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
一种从党参边角料中制备党参提取物的方法，包括先向党参边角料中加入水、纤维素酶和β‑葡萄糖苷酶，混合均匀后进行酶解反应，然后再用溶剂提取、过滤、浓缩和干燥得到党参提取物。β‑葡萄糖苷酶的加入量是纤维素酶加入量的1‑1.5倍；该方法具有节约资源、成本低、提取物活性成分含量高等优势。
Description
PCT国内申请，说明书已公开。
Claims (6)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2019/074273 WO2020155058A1 (zh) | 2019-01-31 | 2019-01-31 | 一种从党参边角料中制备党参提取物的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112384233A true CN112384233A (zh) | 2021-02-19 |
| CN112384233B CN112384233B (zh) | 2022-07-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980042432.XA Active CN112384233B (zh) | 2019-01-31 | 2019-01-31 | 一种从党参边角料中制备党参提取物的方法 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN112384233B (zh) |
| WO (1) | WO2020155058A1 (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113229306B (zh) * | 2021-04-29 | 2024-02-27 | 焙之道食品(福建)有限公司 | 一种复配预拌粉的制备方法 |
| CN114617951A (zh) * | 2022-04-28 | 2022-06-14 | 中山市康腾医疗高科研究有限公司 | 一种中药复合酶解和多菌协同发酵方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101966224A (zh) * | 2010-10-20 | 2011-02-09 | 无锡正大畜禽有限公司 | 一种黄芪、党参复方提取物的生产方法 |
| CN106176878A (zh) * | 2016-07-11 | 2016-12-07 | 周晗生 | 党参提取物的提取方法 |
| CN108064309A (zh) * | 2017-11-30 | 2018-05-22 | 邦泰生物工程(深圳)有限公司 | 一种酶催化合成人参皂苷Rh2的方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103040902B (zh) * | 2012-12-13 | 2016-07-20 | 大兴安岭林格贝寒带生物科技股份有限公司 | 一种纯化野生党参皂苷的方法 |
| CN105238840A (zh) * | 2015-10-27 | 2016-01-13 | 遵义市倍缘化工有限责任公司 | 酶解制备薯蓣皂素的方法 |
| CN106036982B (zh) * | 2016-07-20 | 2017-10-17 | 湖北中烟工业有限责任公司 | 一种烟用月桂叶提取物的制备方法及其应用 |
-
2019
- 2019-01-31 WO PCT/CN2019/074273 patent/WO2020155058A1/zh active Application Filing
- 2019-01-31 CN CN201980042432.XA patent/CN112384233B/zh active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101966224A (zh) * | 2010-10-20 | 2011-02-09 | 无锡正大畜禽有限公司 | 一种黄芪、党参复方提取物的生产方法 |
| CN106176878A (zh) * | 2016-07-11 | 2016-12-07 | 周晗生 | 党参提取物的提取方法 |
| CN108064309A (zh) * | 2017-11-30 | 2018-05-22 | 邦泰生物工程(深圳)有限公司 | 一种酶催化合成人参皂苷Rh2的方法 |
Non-Patent Citations (4)
| Title |
|---|
| ABQ46916.1: "beta-glucosidase. Glycosyl Hydrolase family 3 [Thermotoga petrophila RKU-1]", 《GENBANK DATABASE》 * |
| 岳显文: "党参酶解提取工艺优化", 《黑龙江医药》 * |
| 文洪宇 等: "应用于中药提取分离中的酶的综述", 《中国医药指南》 * |
| 高建德等: "复合酶辅助提取党参中多糖工艺的优化", 《中成药》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020155058A1 (zh) | 2020-08-06 |
| CN112384233B (zh) | 2022-07-26 |
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Denomination of invention: A method for preparing Codonopsis pilosula extract from Codonopsis pilosula scraps Effective date of registration: 20231214 Granted publication date: 20220726 Pledgee: Shenzhen Rural Commercial Bank Co.,Ltd. Guangming Sub branch Pledgor: BONTAC BIO-ENGINEERING (SHENZHEN) Co.,Ltd. Registration number: Y2023980071735 |
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