CN112137937A

CN112137937A - Active composition with function of delaying skin aging and application thereof

Info

Publication number: CN112137937A
Application number: CN202011201906.1A
Authority: CN
Inventors: 刘玉亮; 苏荣生; 姜山山; 邓静
Original assignee: Chuxiang Shanghai Biotechnology Co ltd
Current assignee: Chuxiang Shanghai Biotechnology Co ltd
Priority date: 2020-11-02
Filing date: 2020-11-02
Publication date: 2020-12-29
Anticipated expiration: 2040-11-02
Also published as: CN112137937B

Abstract

The invention relates to the technical field of cosmetics, in particular to an active composition with a function of delaying skin aging and application thereof. An active composition with the function of delaying skin aging comprises rosa canina fruit extract, tremella polysaccharide and sodium hyaluronate, wherein the weight ratio of the rosa canina fruit extract to the tremella polysaccharide to the sodium hyaluronate is (0.5-10): (0.5-10): (1-6). According to the invention, the rosa canina fruit extract, the tremella polysaccharide and the sodium hyaluronate are compounded according to a certain proportion, so that the obtained composition is good in stability, safe and free of stimulation, and has a good antioxidant effect due to the synergistic effect of the compositions, thereby being beneficial to relieving skin aging.

Description

Active composition with function of delaying skin aging and application thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to an active composition with a function of delaying skin aging and application thereof.

Background

Skin aging is classified into: intrinsic aging and extrinsic aging. Endogenous aging refers to natural aging of the skin with aging, which is manifested by fine wrinkles, decreased elasticity, skin laxity, etc.; the most prominent causes of extrinsic aging are photoaging due to sun exposure, manifested by wrinkles, loose skin, roughness, pale yellow or sallow skin discoloration, telangiectasia, formation of pigmented spots, etc. The theory of free radical theory has gained wide acceptance as a theory for delaying skin aging, namely: the natural ability of the human body to combat free radicals is constantly decreasing, the human cells are aging and the skin becomes rough. Under normal conditions, the human body has an antioxidant defense system capable of maintaining a balance state between oxidation and antioxidation, so as to slow down the generation of active oxygen and free radicals. However, ultraviolet radiation, air pollution, environmental pollutants such as chemical substances and the like in the environment can stimulate the human body to continuously generate free radicals, and the large amount of generated free radicals can reduce the antioxidant defense capability of the skin, cause the damage of the skin, and cause the skin aging phenomena such as dark lines, fine lines, wrinkles and the like. Photoaging causes an increase in the activity of skin elastase. Elastase is distributed in various tissues and cells, and the increase of elastase activity can cause the hydrolysis of skin elastic fibers, thereby accelerating the decomposition of collagen and elastin in dermis, leading the skin to lose elasticity, and causing skin aging phenomena such as wrinkles and the like.

Rosa Canina (Latin name: Rosa Canina L.) is a plant of the genus Rosa of the class Dicotyledoneae, and is named because the shape of bud is similar to that of Cibotium caninum when bud extraction is carried out. The Rosa canina fruit is the fruit of Rosa canina, and contains main active ingredients such as flavonoid, tannin, anthocyanin, phenolic compounds, fatty oil, organic acid and inorganic compounds. Has a long-standing traditional application in folk medicine. Rosa canina has been used to treat a variety of diseases including ear, nose and throat problems. In europe, rosa canina fruit is used as a laxative, diuretic, anti-gout and anti-rheumatic drug.

The invention provides an active composition with a function of delaying skin aging, which consists of rosa canina fruit extract, tremella polysaccharide and sodium hyaluronate, has a good antioxidant effect, is beneficial to relieving skin aging, is safe and non-irritant, and has a synergistic effect, so that the function of delaying aging is obviously improved.

Disclosure of Invention

In order to solve the above technical problems, a first aspect of the present invention provides an active composition having a function of delaying skin aging, comprising the following components: rosa canina fruit extract, Tremella polysaccharide and sodium hyaluronate.

As a preferable technical scheme of the invention, the weight ratio of the rosa canina fruit extract, the tremella polysaccharide and the sodium hyaluronate is (0.5-10): (0.5-10): (1-6).

According to a preferable technical scheme of the invention, the weight ratio of the rosa canina fruit extract, the tremella polysaccharide and the sodium hyaluronate is 1: 1: (0.5-0.6).

As a preferable technical scheme of the invention, the preparation method of the rosa canina fruit extract comprises the following steps:

(1) freeze-drying Rosa canina fruit, pulverizing into powder, and sieving with 400-1000 mesh sieve to obtain Rosa canina fruit jelly dry powder;

(2) washing the Rosa canina fruit jelly dry powder with an alcohol aqueous solution for several times, collecting the washing liquid, placing in a refrigerator at-5-5 deg.C, and standing for 10-30 hr; taking out, filtering to obtain filtrate I, and drying at 40-60 deg.C to obtain dried substance;

(3) adding water into the dried product to prepare a solution, placing the solution in a refrigerator with the temperature of between 5 ℃ below zero and 5 ℃, and standing the solution for 10 to 30 hours; taking out and filtering to obtain a filtrate II, extracting for a plurality of times by using an ester organic solvent, collecting an organic phase extract, and then removing the solvent to obtain the organic phase-free water-based paint.

In a preferred embodiment of the present invention, the alcohol volume concentration in the alcohol aqueous solution is not less than 70%.

In a preferred embodiment of the present invention, the drying process is drying under reduced pressure.

In a preferred embodiment of the present invention, the ratio of the dry matter to the water is 1: (10-100); the unit of the feed-liquid ratio is g/mL.

As a preferred technical scheme of the invention, the sodium hyaluronate is a mixture of high molecular sodium hyaluronate, medium molecular sodium hyaluronate and low molecular sodium hyaluronate; the molecular weight of the high molecular sodium hyaluronate is not less than 160 ten thousand daltons; the molecular weight of the middle molecule sodium hyaluronate is 20-160 ten thousand daltons; the molecular weight of the low molecular weight sodium hyaluronate is 1 ten thousand to 20 ten thousand daltons.

The second aspect of the invention provides application of the active composition with the function of delaying skin aging, which is applied to the fields of skin care products and cosmetics.

As a preferable technical scheme, the content of the active composition in the skin care products and the cosmetics is 10-30 wt%.

Advantageous effects

The invention provides a composition with a function of delaying skin aging, which consists of rosa canina fruit extract, tremella polysaccharide and sodium hyaluronate.

Detailed Description

The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

The first aspect of the invention provides an active composition with the function of delaying skin aging, which comprises the following components: rosa canina fruit extract, Tremella polysaccharide and sodium hyaluronate.

In a preferred embodiment, the weight ratio of the rosa canina fruit extract, the tremella polysaccharide and the sodium hyaluronate is (0.5-10): (0.5-10): (1-6).

In a more preferred embodiment, the weight ratio of rosa canina fruit extract, tremella polysaccharide and sodium hyaluronate is 1: 1: (0.5-0.6).

Rosa canina fruit extract

Rosa canina (scientific name: Rosa canina L.): is a plant of the genus Rosa of the class Dicotyledoneae, and is named as "Canine Rose" because the bud is shaped like dog tooth when sprouting. Also called dog-tooth rose, belongs to shrub plants, and the branches and stems are arched or sprawl; has 5 petals and has male stamen. The ovary has one or more, and is composed of separated or synthesized carpel, and is located in the flower pot, so that the female organ has one more protective barrier. The flowering period is 5 months. The flower has sweet faint scent and the color is from white to dark pink. Solitary or clustered; the rose hip is vermilion. Shrubs growing in hedgerows and groves are a common species in europe, growing in temperate regions around the world.

The preparation method of the rosa canina fruit extract comprises the following steps:

In a preferred embodiment, the alcohol concentration in the aqueous solution of alcohol is not less than 70% by volume.

As the alcohol, there are no particular limitations, and methanol, ethanol, n-propanol, isobutanol, and the like can be mentioned.

In a more preferred embodiment, the aqueous alcohol solution is an acidic aqueous alcohol solution.

In a preferred embodiment, the acid is present in a concentration of not higher than 0.5% by volume.

As the acid, there are no particular limitations, and hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, tartaric acid, and the like can be mentioned.

In a more preferred embodiment, the aqueous alcohol solution is an aqueous solution of 80% methanol by volume and 0.1% hydrochloric acid by volume.

In a preferred embodiment, the drying treatment is drying under reduced pressure.

The inventors found that the dried product obtained by drying the extract at 40-60 deg.C under reduced pressure can retain the active ingredient of Rosa canina fruit to the maximum extent and improve the extraction rate of the active ingredient. The inventor thinks that the possible reason is that when the lower drying temperature is adopted, the interface temperature and the surface temperature of the extracting solution are lower due to the smaller heat flux, so that the internal water slowly escapes and is dried, the integrity of the inherent structure of the active ingredients can be better maintained, the damage to the tissue structure of the active ingredients such as pectin, vitamin and the like is reduced, and the physiological activity of the rosa canina fruit is improved; meanwhile, under the condition of reduced pressure, the gas in the extracting solution is pumped out while water is evaporated, so that the dried substance has a uniform, loose and porous structure, the mass transfer resistance of the effective components to the extracting medium from the intracellular direction is reduced, a channel is provided for the flowing and discharging of the later-stage extracting solvent, the contact area of the effective components and the solvent is increased, the quick dissolution of the effective components is facilitated, and the extraction rate and the physiological activity are further improved. Particularly, the damage rate of the active ingredients in the treatment process is minimized by matching with a freeze-drying treatment method.

In a preferred embodiment, the ratio of the dry matter to the water is 1: (10-100); the unit of the feed-liquid ratio is g/mL.

In a more preferred embodiment, the ratio of dry matter to water is 1: 30, of a nitrogen-containing gas; less than 1: the effective components are not completely extracted at 30 days; higher than 1: at 30 f, the amount of the effective components dissolved in water tends to be large in the subsequent organic solvent extraction process, resulting in loss.

In a preferred embodiment, the organic ester-based solvent is not particularly limited, and ethyl acetate, ethyl valerate, ethyl propionate, and the like may be mentioned.

In a preferred embodiment, the specific method of removing the solvent is not particularly limited, and distillation under reduced pressure and the like can be mentioned.

Hyaluronic acid sodium salt

Sodium hyaluronate of the formula (C)₁₄H₂₀NO₁₁Na)_nIt is an inherent component in human body, is a kind of dextran aldehyde acid, has no species specificity, and is widely present in placenta, amniotic fluid, crystalline lens, articular cartilage, dermal layer of skin and other tissues; it is distributed in the cytoplasm and the intercellular substance of the organ, and plays roles of lubricating and nourishing the cells contained therein and the cell organs themselves, and provides a microenvironment for cell metabolism.

In the invention, the sodium hyaluronate is a mixture of high molecular sodium hyaluronate, medium molecular sodium hyaluronate and low molecular sodium hyaluronate; the molecular weight of the high molecular sodium hyaluronate is not less than 160 ten thousand daltons; the molecular weight of the middle molecule sodium hyaluronate is 20-160 ten thousand daltons; the molecular weight of the low molecular weight sodium hyaluronate is 1-20 ten thousand daltons.

In a preferred embodiment, the weight ratio of the high molecular sodium hyaluronate, the medium molecular sodium hyaluronate and the low molecular sodium hyaluronate is 1: (1-5): (0.1-1).

In a more preferred embodiment, the weight ratio of the high molecular sodium hyaluronate, the medium molecular sodium hyaluronate and the low molecular sodium hyaluronate is 1: 3: 0.4.

in the present invention, the source of the sodium hyaluronate is not particularly limited, and Shandong focused Biotech Co., Ltd.

The inventor finds that through reasonable compounding of sodium hyaluronate with different molecular weights, the effects of moisturizing and hydrating the composition, removing oxygen radicals, delaying skin aging and increasing skin elasticity can be improved, the transparent absorptivity of the composition can be effectively improved, the irritation of the composition is reduced, the stability of the composition is improved, and the composition is suitable for protecting the skin in different seasons. The inventor believes that the possible reasons are that the low molecular weight sodium hyaluronate can soften the stratum corneum with water and can also be used as a medium to carry other nutrients into the skin, promote the supply of skin nutrients and promote the transdermal absorption of active ingredients; the high molecular weight sodium hyaluronate can form a compact biological moisturizing film on the surface of skin to prevent the loss of active ingredients, and the compound use of the two kinds of sodium hyaluronate can effectively improve the absorption of the active ingredients by the skin and improve the use effect; in addition, the sodium hyaluronate with medium molecular weight has good moisture retention and lubricity, has the functions of slow release and stable emulsification in the composition, and improves the stability of effective components, especially the stability of active components such as vitamin C in the rosa canina fruit extract through intermolecular force. In addition, the moisturizing property of the high molecular weight sodium hyaluronate is not influenced by the relative humidity of the surrounding environment, and a compact moisturizing film is formed on the surface of the skin, so that the composition is suitable for various seasons and environments with different humidity.

Tremella polysaccharide

Tremella polysaccharide (Tremellam) is an acidic heteropolysaccharide obtained from fruiting body of Tremella fuciformis Berk, and has main chain structure of mannan connected by alpha- (1 → 3) glycosidic bond, and branched chain composed of glucuronic acid and xylose. The main chain structure of the acidic heteropolysaccharide existing in submerged fermentation sporophyte of Tremella is similar to that described above, and only the branched chain is different.

In the present invention, the source of the tremella polysaccharide is not particularly limited, and Shandong focus biotechnology limited may be mentioned.

The inventor finds that the compound with a certain amount of tremella polysaccharide can not only improve the effect of delaying skin aging, but also obviously improve the storage stability of the composition at different temperatures. The inventors believe that a possible reason is that tremella polysaccharide can inhibit droplet movement by raising the viscosity of the aqueous solution, thereby reducing instability of the composition. The main chain structure of the tremella polysaccharide is mannan connected by alpha- (1 → 3) glycosidic bond, the branched chain is composed of glucuronic acid and xylose, the molecule contains a large amount of hydrophilic groups (hydroxyl, carboxyl, amino and the like), the hydrophilic groups can directly enter a water phase, the stability of emulsion is kept under the action of steric hindrance, and the possible layering or aggregation in the emulsion is avoided. In addition, the tremella polysaccharide and the high-molecular sodium hyaluronate are compounded to generate a synergistic effect, the hydration capability of skin cells is obviously enhanced, the water content of the stratum corneum of the skin is increased, a layer of moisturizing and water-locking protective film with a certain thickness can be formed on the surface of the skin, the percutaneous loss of active ingredients and water molecules can be reduced, the double effects of water retention and water locking are achieved, and the skin feels moist after the skin-care cream is used, does not dry, is not tight, and is smooth in skin feel.

The inventor also finds that the complex use of the rosa canina fruit and the tremella polysaccharide can promote the transdermal absorption of active ingredients of the rosa canina fruit extract, has the capability of removing superoxide radicals and hydroxyl radicals, prevents the skin from aging, increases the skin cell activity, promotes the skin regeneration, delays the skin aging, repairs the photodamaged skin, improves the skin nutrient metabolism, makes the skin tender and smooth, removes wrinkles, increases the elasticity and prevents the aging by inhibiting the peroxidation of cell lipid.

In the invention, the preparation method of the active composition with the function of delaying skin aging comprises the following steps: mixing Rosa canina fruit extract, Tremella polysaccharide and sodium hyaluronate uniformly.

In a preferred embodiment, the active composition is present in the skin care product, cosmetic, from 10 to 30% by weight.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the starting materials used are all commercially available, unless otherwise specified.

Examples

Example 1

Example 1 provides an active composition with a function of delaying skin aging, and the active composition is prepared from the following raw materials in percentage by weight: 10% of rosa canina fruit extract, 10% of tremella polysaccharide, 6% of sodium hyaluronate and 100% of water; the weight ratio of the high molecular sodium hyaluronate to the medium molecular sodium hyaluronate to the low molecular sodium hyaluronate is 1: 3: 0.4; the tremella polysaccharide and the hyaluronic acid are purchased from Shandong focus biotechnology limited company;

(1) freeze-drying Rosa canina fruit, crushing into powder, and sieving with 800 mesh sieve to obtain Rosa canina fruit jelly dry powder;

(2) ultrasonically washing the Rosa canina fruit jelly dry powder with aqueous solution of 80% methanol and 0.1% hydrochloric acid, collecting the washing solution, placing in a refrigerator at 4 deg.C, and standing for 24 hr; taking out, filtering to obtain filtrate I, and drying under reduced pressure at 50 deg.C to obtain dried substance;

(3) adding water into the dried product to prepare a solution, wherein the ratio of the material to the liquid is 1: 30(g/mL), placing in a refrigerator at 4 ℃, and standing for 24 hours; taking out and filtering to obtain a filtrate II, extracting for 3 times by using ethyl acetate, collecting an extract liquor II, and then carrying out reduced pressure distillation to obtain the product.

The preparation method of the active composition with the function of delaying skin aging comprises the following steps: mixing Rosa canina fruit extract, Tremella polysaccharide and sodium hyaluronate uniformly.

Example 2

Example 2 differs from example 1 in that the raw materials for preparing the active composition comprise the following components in percentage by weight: 0.5 percent of rosa canina fruit extract, 0.5 percent of tremella polysaccharide, 1 percent of sodium hyaluronate and water for 100 percent.

Example 3

Example 3 differs from example 1 in that the raw materials for preparing the active composition comprise the following components in percentage by weight: 0.5% of rosa canina fruit extract, 0.5% of tremella polysaccharide, 1% of sodium hyaluronate and water to make up to 100%; the Rosa canina fruit extract is commercially available.

Examples 4 to 6

Examples 4 to 6 each provide a cleansing liquid containing the active composition having the function of delaying skin aging, and the specific components of the essence are shown in table 1.

Comparative examples 1 to 3

Comparative examples 1-3 provide a serum having the specific composition shown in table 1.

Table 1 details of the components and contents of the essences described in examples 4 to 6 and comparative examples 1 to 3

The preparation method of the essence comprises the following steps: mixing deionized water, vitamin E acetate, glycerol, 1, 3-propylene glycol, PEG-40 hydrogenated castor oil, dipotassium glycyrrhizinate and octyl glycol, and stirring at 75 deg.C; cooling to 50 deg.C, adding the active composition and PEG-100, and mixing; adding triethanolamine to adjust pH to 5.5-7.0; cooling to below 40 deg.C, adding phenoxyethanol, ethylhexyl glycerol and essence, and mixing.

Performance testing

Detection of radical scavenging Activity by DPPH method

(1) The test principle is as follows: 1, 1-Diphenyl-2-trinitrophenylhydrazine (1, 1-Diphenyl-2-piperidinylhydrazyl, DPPH) is a stable nitrogen center organic free radical, and an ethanol solution of the stable nitrogen center organic free radical is dark purple and has strong absorption at about 517 nm; in the presence of free radical scavengers, DPPH absorption is diminished by pairing with its single electron, and gradually disappears. The degree of color fading of the DPPH ethanol solution is in a quantitative relation with the number of electrons received by the DPPH ethanol solution, so that a spectrophotometer can be used for carrying out rapid quantitative analysis, detecting the condition of scavenging free radicals and evaluating the capacity of the antioxidant for scavenging the free radicals.

(2) The test method comprises the following steps:

preparing a DPPH ethanol solution: 6mg of DPPH was weighed out accurately, dissolved in 95% ethanol and placed in a 50mL volumetric flask, and dissolved with the aid of ultrasound. The application method comprises diluting with 95% ethanol for four times to obtain DPPH ethanol solution with concentration of 3mg/100mL (76 μ M), and storing in dark (0-4 deg.C).

Pretreating a sample to be detected: the ascorbic acid standards of examples 1-3 were dissolved in deionized water and diluted to the appropriate concentration with deionized water for use.

The experimental steps are as follows: adding 2mL of sample solution to be detected into a test tube, then adding 2mL of ethanol solution of LDPPH, and uniformly mixing. Reacting in dark place at room temperature for 30min in the dark place in a dark place, and measuring absorbance Abs at 525 nm; except for the sample group, a sample solvent control group, a blank control group and a solvent control group (solvent of DPPH) are respectively arranged, and the positive control group is an ascorbic acid group.

Clearance I (%) ═ 1- (T-T)₀)/(C-C₀)]×100％

In the formula:

T₀: adding the absorbance of the sample solution to be detected with equal volume of 95% ethanol (sample solvent control group);

t: adding the absorbance of the sample solution to be detected and an isopyknic DPPH solution (sample group);

C₀: absorbance of distilled water plus equal volume of 95% ethanol (solvent control);

c: absorbance of distilled water plus an equal volume of DPPH solution (blank control).

The test results are shown in Table 2.

TABLE 2 detection results of activity of scavenging free radicals by DPPH method

As can be seen from Table 2, the active composition has a DPPH radical scavenging effect. Compared with a blank control group, the active composition has stronger capacity of scavenging free radicals, and the higher the content of the active ingredients, the higher the DPPH clearance rate.

2. Detection of Elastase Activity inhibition

The experimental steps are as follows: mu.L of a sample solution and 130. mu.L of a 0.1M Tris-HCl buffer solution (pH8.0) containing 1.015mM of the reaction substrate Succ-Ala-Ala-p-nitroanilide were added to a 96-well plate, incubated at 25 ℃ for 5 minutes, 15. mu.L of an elastase solution (0.5U/mL) was added, and incubation at 25 ℃ was continued for 30 minutes, and then absorbance A410 at a wavelength of 410nm was measured by a microplate reader, the sample solution including the solution prepared in examples 1-3 and an Elastatin solution (both diluted with deionized water to an appropriate concentration), wherein Elastatin solution (Elastatin enzyme inhibitor, purchased) was used as a positive control group. The absorbance was also measured as a reference solution by replacing the sample aqueous solution with deionized water.

The calculation formula of the elastase activity inhibition rate is as follows: inhibition ratio (%) ═ a₀-(A₄₁₀-B))/A₀×100％；

Wherein:

A₀: absorbance of a reference solution;

A₄₁₀: is the absorbance of the sample solution;

b: absorbance of sample blank solution.

The test results are shown in Table 3.

TABLE 3 measurement results of Elastase Activity inhibition

Examples	Concentration (μ g/mL)	Elastase inhibition%
			Example 1	10	80
Example 2	10	43
			Example 3	10	34
Elastatinal	0.2	38

As can be seen from table 3, the active composition has an elastase inhibitory effect, and compared to the blank control group, the active composition has a stronger elastase inhibitory effect, and the higher the content of the active ingredient is, the higher the inhibitory effect is.

3. Skin texture test

Selecting the left and right cheekbones of the face of a subject, the left and right cheeks at the intersection of the nose tip and the pupil, and the left and right sides at the intersection of the temple and the mouth corner as test areas, firstly, measuring the skin texture of the test part before applying the cosmetics by a technician by using a visia facial image analyzer, measuring five times at each point, and taking an average value. The subjects tested skin texture again after four weeks of continuous use of the cosmetics in the examples and comparative examples. The number of each measurement at the test site of the subject was counted, and the change law of the skin texture was analyzed, thereby determining the efficacy of the example and comparative example cosmetics for the improvement of the skin texture.

The test results are shown in Table 4.

TABLE 4 skin texture test results

4. Anti-wrinkle experiment on human skin

The anti-wrinkle test of human skin is the most direct method for testing the anti-wrinkle effect. The test population is female wrinkled people with the average age of 35-45 years. The left and right cheekbones of the face, the left and right cheeks at the intersection of the nose tip and the pupil, and the left and right sides at the intersection of the temple and the mouth corner of the subject were selected as the test areas. The elasticity value of the applied part of the subject was measured using a skin elasticity tester (MPA580) before the test. The gel is applied twice a day in the morning and at night, and the photo before application is used as a control. During the experiment, the subject was unable to apply any other cosmetic. After the subjects continued to use the anti-wrinkle cosmetic for four weeks, the skin elasticity values before and after the use of the example and comparative cosmetics were compared. And counting the values measured at each time of the experimental part of the subject, and analyzing the skin elasticity change rule of the subject to evaluate the skin elasticity change condition of the subject.

The test results are shown in Table 5.

TABLE 5 anti-wrinkle test results on human skin

As shown in tables 4 and 5, the skin elasticity and texture improvement effect of the subjects of comparative examples 1 to 3 is general after 4 weeks of use, while the skin elasticity and texture improvement effect of the subjects of examples 4 to 6 is obvious after 4 weeks of use, which indicates that the rosa canina fruit extract of the present invention can fully exert its antioxidant activity, and has synergistic effect with tremella polysaccharide and hyaluronic acid, and the anti-aging effect of the product is obviously improved.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. An active composition with the function of delaying skin aging, which is characterized by comprising the following components: rosa canina fruit extract, Tremella polysaccharide and sodium hyaluronate.

2. The active composition with the function of delaying skin aging according to claim 1, wherein the weight ratio of the rosa canina fruit extract, the tremella polysaccharides and the sodium hyaluronate is (0.5-10): (0.5-10): (1-6).

3. The active composition according to claim 1, wherein the weight ratio of the rosa canina fruit extract, the tremella polysaccharide and the sodium hyaluronate is 1: 1: (0.5-0.6).

4. The active composition with the function of delaying skin aging as claimed in claim 1, wherein the preparation method of the rosa canina fruit extract comprises the following steps:

5. The active composition with skin aging delaying function according to claim 4, wherein the volume concentration of the alcohol in the alcohol aqueous solution is not less than 70%.

6. The active composition with the function of delaying skin aging according to claim 4, wherein the drying treatment is reduced pressure drying.

7. The active composition with the function of delaying skin aging according to claim 4, wherein the feed-liquid ratio of the dry matter to the water is 1: (10-100); the unit of the feed-liquid ratio is g/mL.

8. The active composition with the function of delaying skin aging according to claim 1, wherein the sodium hyaluronate is a mixture of high molecular sodium hyaluronate, medium molecular sodium hyaluronate and low molecular sodium hyaluronate; the molecular weight of the high molecular sodium hyaluronate is not less than 160 ten thousand daltons; the molecular weight of the middle molecule sodium hyaluronate is 20-160 ten thousand daltons; the molecular weight of the low molecular weight sodium hyaluronate is 1 ten thousand to 20 ten thousand daltons.

9. Use of the active composition with skin aging delaying function according to any one of claims 1 to 8, characterized by being applied to the fields of skin care products and cosmetics.

10. The use of the active composition with the function of delaying skin aging according to claim 9, characterized in that the content of the active composition in skin care products and cosmetics is 10-30 wt%.