CN112137937B

CN112137937B - Active composition with skin aging delaying function and application thereof

Info

Publication number: CN112137937B
Application number: CN202011201906.1A
Authority: CN
Inventors: 刘玉亮; 苏荣生; 姜山山; 邓静
Original assignee: Chuxiang Shanghai Biotechnology Co ltd
Current assignee: Chuxiang Shanghai Biotechnology Co ltd
Priority date: 2020-11-02
Filing date: 2020-11-02
Publication date: 2023-05-02
Anticipated expiration: 2040-11-02
Also published as: CN112137937A

Abstract

The invention relates to the technical field of cosmetics, in particular to an active composition with a skin aging delaying function and application thereof. An active composition with skin aging delaying function comprises fructus Rosae Davuricae extract, tremella polysaccharide and sodium hyaluronate (weight ratio of 0.5-10): (0.5-10): (1-6). According to the invention, the fructus rosae canicae extract, the tremella polysaccharide and the sodium hyaluronate are compounded according to a certain proportion, so that the obtained composition has good stability, safety and no stimulation, and the compositions have synergistic effect, have good antioxidant effect and are beneficial to relieving skin aging.

Description

Active composition with skin aging delaying function and application thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to an active composition with a skin aging delaying function and application thereof.

Background

Skin aging is classified as: endogenous aging and exogenous aging. Endogenous aging refers to natural aging of skin with aging, which is manifested by fine wrinkles, reduced elasticity, and loose skin; the most important causes of extrinsic aging are photoaging due to sun exposure, manifested by wrinkles, skin sagging, rough, yellowish or yellowish skin discoloration, telangiectasia, pigmentation, etc. The theory of free radical theory is widely accepted as a theory of delaying skin aging, namely: the natural anti-free radical capability of the human body is continuously reduced, the human cells are aged, and the skin is roughened. Under normal conditions, the human body is provided with an antioxidant defense system capable of maintaining the balance between oxidation and antioxidation, so as to slow down the generation of active oxygen and free radicals. However, environmental pollutants such as ultraviolet radiation, air pollution, chemical substances and the like in the environment can stimulate a human body to continuously generate free radicals, and the generated free radicals in a large quantity can reduce the oxidation resistance and the defense capacity of skin, so that the skin is damaged, and skin aging phenomena such as dark lines, fine lines, wrinkles and the like appear. Photoaging causes an increase in the activity of skin elastase. Elastase is distributed in various tissues and cells, and the increase of elastase activity can cause the hydrolysis of skin elastic fibers, so that the decomposition of collagen and elastin in dermis is accelerated, the skin loses elasticity, and skin aging phenomena such as wrinkles and the like occur.

Rosa Canina (Latin name: rosa Canina L.) is a dicotyledonous plant of Rosa genus, and is named only when the bud is shaped like a dog tooth when it is germinated. Fructus Rosae Davuricae is fruit of Rosa canina, and contains main active ingredients such as flavonoids, tannin, anthocyanin, phenolic compounds, fatty oil, organic acid and inorganic compounds. Has long-lasting traditional use in folk medicine. Rosa canina has been used to treat a variety of diseases including ear, nose and throat problems. In europe, rosa canina is used as a laxative, diuretic, anti-gout and antirheumatic drug.

The invention provides an active composition with a skin aging delaying function, which consists of a dog rose fruit extract, tremella polysaccharide and sodium hyaluronate, has a good antioxidation effect, is beneficial to relieving skin aging, is safe and has no stimulation, synergistic effect is generated between the compositions, and the aging delaying effect is obviously improved.

Disclosure of Invention

In order to solve the technical problem of the above specification, a first aspect of the present invention provides an active composition having a skin aging delaying function, the active composition comprising the following components: fructus Rosae Davuricae extract, tremella polysaccharide and sodium hyaluronate.

As a preferable technical scheme of the invention, the weight ratio of the dog rose fruit extract, tremella polysaccharide and sodium hyaluronate is (0.5-10): (0.5-10): (1-6).

As a preferable technical scheme of the invention, the weight ratio of the dog rose-hip extract, the tremella polysaccharide and the sodium hyaluronate is 1:1: (0.5-0.6).

As a preferable technical scheme of the invention, the preparation method of the Rosa canina fruit extract comprises the following steps:

(1) Freeze-drying fructus Rosae Davuricae, pulverizing into powder, and sieving with 400-1000 mesh sieve to obtain fructus Rosae Davuricae powder;

(2) Washing the dog rose jelly dry powder with an alcohol aqueous solution for a plurality of times, collecting washing liquid, and placing in a refrigerator at the temperature of-5 to 5 ℃ for standing for 10 to 30 hours; taking out and filtering to obtain filtrate I, and drying at 40-60deg.C to obtain dried product;

(3) Adding water into the dried product to prepare a solution, and placing the solution in a refrigerator at the temperature of-5 to 5 ℃ for standing for 10 to 30 hours; taking out and filtering to obtain filtrate II, extracting with ester organic solvent for several times, collecting organic phase extractive solution, and removing solvent.

As a preferable technical scheme of the invention, the volume concentration of the alcohol in the aqueous solution of the alcohol is not less than 70%.

As a preferable embodiment of the present invention, the drying treatment is reduced pressure drying.

As a preferable technical scheme of the invention, the feed liquid ratio of the dry matter to the water is 1: (10-100); the unit of the feed liquid ratio is g/mL.

As a preferable technical scheme of the invention, the sodium hyaluronate is a mixture of high molecular sodium hyaluronate, medium molecular sodium hyaluronate and low molecular sodium hyaluronate; the molecular weight of the high molecular sodium hyaluronate is not less than 160 ten thousand daltons; the molecular weight of the medium molecular sodium hyaluronate is 20 ten thousand-160 ten thousand daltons; the molecular weight of the low molecular weight sodium hyaluronate is 1 ten thousand to 20 ten thousand daltons.

The second aspect of the invention provides application of the active composition with the function of delaying skin aging, and the active composition is applied to the fields of skin care products and cosmetics.

As a preferred technical scheme of the invention, the content of the active composition in skin care products and cosmetics is 10-30wt%.

Advantageous effects

The invention provides a composition for delaying skin aging, which consists of a dog-tooth rose-hip extract, tremella polysaccharide and sodium hyaluronate, wherein the dog-tooth rose-hip extract, the tremella polysaccharide and the sodium hyaluronate are compounded according to a certain proportion, and the obtained composition has good stability, safety and no stimulation, has synergistic effect, has good antioxidant effect and is beneficial to relieving skin aging.

Detailed Description

The contents of the present invention can be more easily understood by referring to the following detailed description of preferred embodiments of the present invention and examples included. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprising," "including," "having," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified element, step or component. If used in a claim, such phrase will cause the claim to be closed, such that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the claim body, rather than immediately following the subject, it is limited to only the elements described in that clause; other elements are not excluded from the stated claims as a whole.

When an equivalent, concentration, or other value or parameter is expressed as a range, preferred range, or a range bounded by a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when ranges of "1 to 5" are disclosed, the described ranges should be construed to include ranges of "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a numerical range is described herein, unless otherwise indicated, the range is intended to include its endpoints and all integers and fractions within the range.

The singular forms include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or event may or may not occur, and that the description includes both cases where the event occurs and cases where the event does not.

Approximating language, in the specification and claims, may be applied to modify an amount that would not limit the invention to the specific amount, but would include an acceptable portion that would be close to the amount without resulting in a change in the basic function involved. Accordingly, the modification of a numerical value with "about", "about" or the like means that the present invention is not limited to the precise numerical value. In some examples, the approximating language may correspond to the precision of an instrument for measuring the value. In the present specification and claims, the range limitations may be combined and/or interchanged, such ranges including all the sub-ranges contained therein if not expressly stated.

In a first aspect the present invention provides an active composition having the function of delaying skin ageing, the active composition comprising the following components: fructus Rosae Davuricae extract, tremella polysaccharide and sodium hyaluronate.

In a preferred embodiment, the weight ratio of the dog rose hip extract, tremella polysaccharide and sodium hyaluronate is (0.5-10): (0.5-10): (1-6).

In a more preferred embodiment, the weight ratio of the dog rose hip extract, tremella polysaccharide and sodium hyaluronate is 1:1: (0.5-0.6).

Rosa canina fruit extract

Rosa canina (academic name: rosa canina l.): is a dicotyledonous plant of Rosa genus, and is called "Canine Rose" because the shape of the bud resembles that of a dog tooth when the bud is drawn. Also called Rosa canina, belonging to shrub plants, the branches and stems are arched or vining; there are 5 petals and a male stamen. The ovary has one or more, and consists of isolated or synthetic carpels, and is located in the flower pot, so that its female organ is further provided with a protective barrier. The flowering period is 5 months. Flowers have a sweet fragrance, and the color of the flowers is changed from white to dark pink. Single or clustered; the fructus Rosae Davuricae is vermilion. Shrubs growing in hedges and in the woody clusters are a common species in europe, longer than temperate regions around the world.

In the invention, the preparation method of the Rosa canina fruit extract comprises the following steps:

In a preferred embodiment, the alcohol is present in the aqueous alcohol solution at a concentration of not less than 70% by volume.

As the alcohol, there are no particular restrictions, and methanol, ethanol, n-propanol, isobutanol, and the like can be mentioned.

In a more preferred embodiment, the aqueous solution of an alcohol is an acidic aqueous solution of an alcohol.

In a preferred embodiment, the acid is present in a concentration of not more than 0.5% by volume.

As the acid, hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, tartaric acid, and the like can be mentioned without particular limitation.

In a more preferred embodiment, the aqueous solution of alcohol is an aqueous solution of methanol at a concentration of 80% by volume with hydrochloric acid at a concentration of 0.1% by volume.

In a preferred embodiment, the drying process is reduced pressure drying.

The inventor finds that the dried product obtained by drying the extracting solution under reduced pressure at 40-60 ℃ can keep the active ingredients of the fructus rosae canina to the greatest extent and improve the extraction rate of the active ingredients. The inventor considers that the possible reason is that when the drying temperature is lower, the interface temperature and the surface temperature of the extracting solution are lower due to the lower heat flux, so that the internal moisture slowly escapes and dries, the inherent structural integrity of the active ingredient can be better maintained, the damage to the tissue structure of the active ingredient such as pectin, vitamin and the like is reduced, and the physiological activity of the dog rose fruits is improved; simultaneously, under the condition of decompression, the water is evaporated and the gas carried in the extracting solution is pumped out together, so that the dried product has a uniform, loose and porous structure, the mass transfer resistance of the active ingredient from the inside of the cell to the diffusion of the extracting medium is reduced, a channel is provided for the flow and the elimination of the extracting solvent in the later stage, the contact area of the active ingredient and the solvent is increased, the quick dissolution of the active ingredient is facilitated, and the extraction rate and the physiological activity are further improved. Especially, the method is matched with a freeze-drying treatment method, and the destruction rate of the treatment process to the active ingredients is minimized.

In a preferred embodiment, the ratio of the dry matter to water is 1: (10-100); the unit of the feed liquid ratio is g/mL.

In a more preferred embodiment, the ratio of the dry matter to water is 1:30; below 1: at 30, the extraction of the effective components is incomplete; higher than 1:30, the dissolution amount of the effective components in water is large in the subsequent organic solvent extraction process, so that the loss is caused.

In a preferred embodiment, the organic ester solvent is not particularly limited, and ethyl acetate, ethyl valerate, ethyl propionate, and the like may be mentioned.

In a preferred embodiment, the specific method for removing the solvent is not particularly limited, and reduced pressure distillation and the like can be mentioned.

Sodium hyaluronate

Sodium hyaluronate with chemical formula (C) ₁₄ H ₂₀ NO ₁₁ Na) _n Is an inherent component in human body, is a glucuronic acid, has no species specificity, and widely exists in placenta, amniotic fluid, lens, articular cartilage, dermis layer of skin and other tissues; it is distributed in cytoplasm and cell interstitium to lubricate and nourish cells and cell organ, and provide cell metabolismIs a micro-environment of (a).

In the invention, the sodium hyaluronate is a mixture of high molecular sodium hyaluronate, medium molecular sodium hyaluronate and low molecular sodium hyaluronate; the molecular weight of the high molecular sodium hyaluronate is not less than 160 ten thousand daltons; the molecular weight of the medium molecular sodium hyaluronate is 20 ten thousand-160 ten thousand daltons; the molecular weight of the low molecular weight sodium hyaluronate is 1 ten thousand to 20 ten thousand daltons.

In a preferred embodiment, the weight ratio of the high molecular sodium hyaluronate, the medium molecular sodium hyaluronate and the low molecular sodium hyaluronate is 1: (1-5): (0.1-1).

In a more preferred embodiment, the weight ratio of the high molecular sodium hyaluronate, the medium molecular sodium hyaluronate and the low molecular sodium hyaluronate is 1:3:0.4.

in the present invention, the source of sodium hyaluronate is not particularly limited, and Shandong focus biotechnology Co., ltd.

The inventor finds that through reasonable compounding of the sodium hyaluronate with different molecular weights, the effects of moisturizing and moisturizing the composition, scavenging oxygen free radicals, delaying skin aging and increasing skin elasticity can be improved, the transparent absorbability of the composition can be effectively improved, the irritation of the composition is reduced, the stability of the composition is improved, and the composition is suitable for protecting the skin in different seasons. The inventors believe that the possible reason is that low molecular weight sodium hyaluronate is capable of water and softening the stratum corneum, and also is capable of carrying other nutrients into the skin as a vehicle, promoting the supply of skin nutrients, and promoting the transdermal absorption of active ingredients; the high molecular weight sodium hyaluronate can form a compact biological moisturizing film on the surface of the skin, so that the loss of active ingredients is prevented, and the compound use of the two sodium hyaluronate can effectively improve the absorption of the skin to the active ingredients and improve the use effect; in addition, the sodium hyaluronate with medium molecular weight has good moisture retention and lubricity, plays roles in slow release and stable emulsification in the composition, and improves the stability of active ingredients, especially the stability of active ingredients such as vitamin C in the Rosa canina fruit extract by intermolecular force. In addition, the moisture retention of the high molecular weight sodium hyaluronate is not affected by the relative humidity of the surrounding environment, and a compact moisture retention film is formed on the skin surface, so that the composition is suitable for environments with different seasons and different humidity.

Tremella polysaccharide

Tremella polysaccharide (Tremella) is an acidic heteropolysaccharide obtained from fruiting body of Tremella (Tremella fuciformisBerk), its main chain structure is mannans connected by alpha- (1→3) glycosidic bond, and branched chain is composed of glucuronic acid and xylose. The acidic heteropolysaccharide existing in the tremella submerged fermentation sporophyte has a main chain structure similar to that described above and only differs in the branched chain.

In the present invention, the source of the tremella polysaccharide is not particularly limited, and Shandong focus biotechnology Co., ltd.

The inventor discovers that the tremella polysaccharide compounded with a certain amount not only can improve the effect of delaying skin aging, but also can remarkably improve the storage stability of the composition at different temperatures. The inventors believe that the possible reason is that tremella polysaccharide can suppress droplet movement by increasing the viscosity of water, thereby reducing the instability of the composition. The main chain structure of tremella polysaccharide is mannans connected by alpha- (1- & gt 3) glycosidic bond, the branched chain is composed of glucuronic acid and xylose, a large number of hydrophilic groups (hydroxyl, carboxyl, amino and the like) are contained in the molecule, and can directly enter into a water phase, so that the stability of emulsion is maintained under the action of steric hindrance, and the condition of layering or aggregation possibly occurring in the emulsion is avoided. In addition, the tremella polysaccharide and the high-molecular sodium hyaluronate are compounded for use, so that a synergistic effect can be generated, the hydration capacity of skin cells is obviously enhanced, the water content of skin cuticle is increased, a moisturizing and water-locking protective film with a certain thickness can be formed on the skin surface, the percutaneous loss of active ingredients and water molecules can be reduced, the double effects of water retention and water locking are also realized, and skin is moist, does not dry, is not tight and is smooth after the tremella polysaccharide and the high-molecular sodium hyaluronate are used.

The inventor also finds that the compound use of the dog rose-hip and tremella polysaccharide can promote the transdermal absorption of active ingredients of the dog rose-hip extract, has the capability of removing superoxide radicals and hydroxyl radicals, prevents skin aging by inhibiting peroxidation of cell lipid, increases skin cell activity, promotes skin regeneration, delays skin aging, repairs light damaged skin, and simultaneously can improve skin nutrition metabolism, tender, smooth, wrinkle-removing, elasticity-increasing and aging-preventing skin.

In the invention, the preparation method of the active composition with the function of delaying skin aging comprises the following steps: mixing fructus Rosae Davuricae extract, tremella polysaccharide and sodium hyaluronate.

In a preferred embodiment, the active composition is present in skin care products, cosmetics in an amount of 10 to 30wt%.

The present invention will be specifically described below by way of examples. It is noted herein that the following examples are given solely for the purpose of further illustration and are not to be construed as limitations on the scope of the invention, as will be apparent to those skilled in the art in light of the foregoing disclosure.

In addition, the raw materials used are commercially available unless otherwise indicated.

Examples

Example 1

Example 1 provides an active composition with skin aging delaying function, which is prepared from the following raw materials in percentage by weight: 10% of dog tooth rose hip extract, 10% of tremella polysaccharide, 6% of sodium hyaluronate and 100% of water; the weight ratio of the high molecular sodium hyaluronate, the medium molecular sodium hyaluronate and the low molecular sodium hyaluronate is 1:3:0.4; the tremella polysaccharide and the hyaluronic acid are purchased from Shandong focus biotechnology limited company;

the preparation method of the Rosa canina fruit extract comprises the following steps:

(1) Freeze-drying fructus Rosae Davuricae, pulverizing into powder, and sieving with 800 mesh sieve to obtain fructus Rosae Davuricae powder;

(2) Ultrasonic washing fructus Rosae Davuricae lyophilized powder with 80% methanol and 0.1% hydrochloric acid water solution for 3 times, collecting the washing liquid, placing in a refrigerator at 4deg.C, and standing for 24 hr; taking out and filtering to obtain filtrate I, and drying under reduced pressure at 50deg.C to obtain dried product;

(3) Adding water into the dried product to prepare a solution, wherein the feed liquid ratio is 1:30 (g/mL) and placed in a refrigerator at 4℃for 24 hours; taking out and filtering to obtain filtrate II, extracting with ethyl acetate for 3 times, collecting extract II, and distilling under reduced pressure.

The preparation method of the active composition with the function of delaying skin aging comprises the following steps: mixing fructus Rosae Davuricae extract, tremella polysaccharide and sodium hyaluronate.

Example 2

Example 2 differs from example 1 in that the active composition is prepared from the following raw materials in percentage by weight: 0.5% of Rosa canina fruit extract, 0.5% of tremella polysaccharide, 1% of sodium hyaluronate and 100% of water.

Example 3

Example 3 differs from example 1 in that the active composition is prepared from the following ingredients in weight percent: 0.5% of dog tooth rose hip extract, 0.5% of tremella polysaccharide, 1% of sodium hyaluronate and 100% of water; the Rosa canina fruit extract is commercially available.

Examples 4 to 6

Examples 4 to 6 each provide a cleansing liquid comprising the active composition having a skin aging delaying function, and specific components of the essence are shown in table 1.

Comparative examples 1 to 3

Comparative examples 1-3 provide a serum with specific compositions as shown in table 1.

TABLE 1 specific Components and content of the essence described in examples 4-6 and comparative examples 1-3

The preparation method of the essence comprises the following steps: mixing deionized water, vitamin E acetate, glycerol, 1, 3-propylene glycol, PEG-40 hydrogenated castor oil, dipotassium glycyrrhizinate and octanediol, and stirring at 75deg.C; cooling to 50 ℃, adding the active composition and PEG-100, and uniformly mixing; adding triethanolamine to adjust pH to 5.5-7.0; cooling to below 40deg.C, adding phenoxyethanol, ethylhexyl glycerol and essence, and mixing.

Performance testing

DPPH method scavenging free radical Activity detection

(1) The test principle is as follows: 1, 1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic radical, and the ethanol solution is dark purple and has strong absorption about 517 nm; when a radical scavenger exists, DPPH absorption is weakened and gradually disappears due to single electron pairing with the radical scavenger. The fading degree of the DPPH ethanol solution is quantitatively related to the number of electrons accepted by the DPPH ethanol solution, so that the rapid quantitative analysis can be carried out by using a spectrophotometer, the free radical removal condition is detected, and the free radical removal capability of the antioxidant is evaluated.

(2) The testing method comprises the following steps:

preparing a DPPH ethanol solution: accurately weighing 6mg DPPH, dissolving with 95% ethanol, and dissolving with ultrasonic assistance in a 50mL volumetric flask. When in use, the solution is diluted four times by 95% ethanol to obtain DPPH ethanol solution with the concentration of 3mg/100mL (76 mu M), and the solution is preserved in a dark place (0-4 ℃).

Pretreatment of a sample to be tested: examples 1-3, the ascorbic acid standard, were dissolved in deionized water and diluted with deionized water to the appropriate concentration for use.

The experimental steps are as follows: and adding 2mL of the sample solution to be detected into a test tube, then adding 2mL of the PPH ethanol solution, and uniformly mixing. The reaction is carried out for 30min at room temperature in dark place and the absorbance Abs is measured at 525 nm; in addition to the sample group, a sample solvent control group, a blank control group and a solvent control group (DPPH solvent) are respectively arranged, and the positive control group is an ascorbic acid group.

Clearance I (%) = [1- (T-T) ₀ )/(C-C ₀ )]×100％

Wherein:

T ₀ : absorbance of the sample solution to be measured with 95% ethanol (sample solvent control group) of equal volume;

t: absorbance (sample group) of the sample solution to be measured added with the equal volume of DPPH solution;

C ₀ : absorbance of distilled water plus equal volume of 95% ethanol (solvent control);

c: absorbance of distilled water plus an equal volume of DPPH solution (blank).

The test results are shown in Table 2.

TABLE 2 DPPH method scavenging free radical Activity assay results

As can be seen from Table 2, the active composition has the effect of scavenging DPPH free radicals. Compared with the blank control group, the active composition has stronger capability of scavenging free radicals, and the higher the content of the active ingredient, the higher the DPPH scavenging rate.

2. Detection of inhibition of elastase Activity

The experimental steps are as follows: mu.L of a sample solution comprising the solutions prepared in examples 1-3 and elastinal solution (both diluted to a suitable concentration with deionization) and 130. Mu.L of 0.1M Tris-HCl buffer (pH 8.0) containing 1.015mM reaction substrate Succ-Ala-Ala-p-nitroanilide were added to a 96-well plate, incubated at 25℃for 5 minutes, 15. Mu.L of elastase solution (0.5U/mL) was added, incubation was continued at 25℃for 30 minutes, and absorbance A410 at a wavelength of 410nm was measured with a microplate reader, wherein the elastinal solution (elastase inhibitor, outsourced) was used as a positive control group. The absorbance was also measured by replacing the aqueous sample solution with deionized water as a reference solution.

The elastase activity inhibition rate is calculated as: inhibition (%) = (a) ₀ -(A ₄₁₀ -B))/A ₀ ×100％；

Wherein:

A ₀ : absorbance of the reference solution;

A ₄₁₀ : is the absorbance of the sample solution;

b: absorbance of sample blank solution.

The test results are shown in Table 3.

TABLE 3 determination of elastase Activity inhibition

Examples	Concentration (μg/mL)	Elastase inhibition%
			Example 1	10	80
Example 2	10	43
			Example 3	10	34
Elastatinal	0.2	38

As can be seen from table 3, the active composition has an elastase inhibitory effect, and the active composition has a stronger elastase inhibitory effect than the blank, and the higher the content of the active ingredient, the higher the inhibitory effect.

3. Skin texture testing

Selecting left and right cheeks at the intersection line of the facial cheeks, the nasal tip and the pupil of a subject, and left and right sides at the intersection line of the temple and the mouth corner of the subject as test areas, firstly, measuring the skin texture degree of the test area by a technician by using a visual facial image analyzer before applying cosmetics, measuring five times per point, and taking an average value. The subjects were again tested for skin texture after four weeks of continuous use of the cosmetics in examples and comparative examples. And counting the numerical value measured by each time of the experimental part of the subject, and analyzing the change rule of the skin texture of the experimental part of the subject, so as to determine the effect of the cosmetics of the examples and the comparative examples on improving the skin texture.

The test results are shown in Table 4.

TABLE 4 skin texture test results

4. Anti-wrinkle test of human skin

The anti-wrinkle test of human skin is the most direct method for testing anti-wrinkle effect. The subject population was wrinkled females with an average age of 35-45 years. The left and right cheeks at the intersection of the facial cheeks, nasal tip and pupil, and the left and right sides at the intersection of the temple and the corner of the mouth of the subject are selected as test areas. The elasticity value of the site of application of the subject was determined prior to testing using a skin elasticity tester (MPA 580). The composition was applied twice a day in the morning and evening, and the photo before application was used as a control. During the experiment, the subjects were unable to apply any other cosmetics. After four weeks of continuous use of the anti-wrinkle cosmetics, the subjects were compared with the skin elasticity values before and after use of the cosmetics of examples and comparative examples. And counting the numerical value measured by the experimental part of the subject each time, and analyzing the skin elasticity change rule to evaluate the skin elasticity change condition.

The test results are shown in Table 5.

TABLE 5 results of anti-wrinkle test on human skin

As shown in tables 4 and 5, the subjects of comparative examples 1 to 3 have general skin elasticity and texture improvement effects after using for 4 weeks, while the subjects of examples 4 to 6 have remarkable skin elasticity and texture improvement effects after using for 4 weeks, which indicates that the Rosa canina fruit extract of the present invention can fully exert its antioxidant activity, and has synergistic effects with tremella polysaccharide and hyaluronic acid, and the anti-aging effect of the product is remarkably improved.

The foregoing examples are illustrative only and serve to explain some features of the method of the invention. The appended claims are intended to claim the broadest possible scope and the embodiments presented herein are merely illustrative of selected implementations based on combinations of all possible embodiments. It is, therefore, not the intention of the applicant that the appended claims be limited by the choice of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within which variations in these ranges should also be construed as being covered by the appended claims where possible.

Claims

1. An active composition with skin aging delaying function is characterized in that,

the active composition comprises the following components: fructus Rosae Davuricae extract, tremella polysaccharide and sodium hyaluronate; the weight ratio of the dog tooth rose hip extract to tremella polysaccharide to sodium hyaluronate is 1:1: (0.5-0.6);

(3) Adding water into the dried product to prepare a solution, and placing the solution in a refrigerator at the temperature of-5 to 5 ℃ for standing for 10 to 30 hours; taking out and filtering to obtain filtrate II, extracting with ester organic solvent for several times, collecting organic phase extractive solution, and removing solvent to obtain the final product;

the sodium hyaluronate is a mixture of high molecular sodium hyaluronate, medium molecular sodium hyaluronate and low molecular sodium hyaluronate; the molecular weight of the high molecular sodium hyaluronate is not less than 160 ten thousand daltons; the molecular weight of the medium molecular sodium hyaluronate is 20 ten thousand-160 ten thousand daltons; the molecular weight of the low molecular weight sodium hyaluronate is 1 ten thousand to 20 ten thousand daltons;

the weight ratio of the high molecular sodium hyaluronate, the medium molecular sodium hyaluronate and the low molecular sodium hyaluronate is 1: (1-5): (0.1-1).

2. The active composition having a function of delaying skin aging according to claim 1, wherein the volume concentration of the alcohol in the aqueous solution of the alcohol is not less than 70%.

3. The active composition having a function of delaying skin aging according to claim 1, wherein the drying treatment is reduced pressure drying.

4. The active composition having a skin aging delaying function according to claim 1, wherein the ratio of the dry matter to water is 1: (10-100); the unit of the feed liquid ratio is g/mL.

5. Use of an active composition with a function of delaying skin aging according to any one of claims 1 to 4, characterized in that it is applied in the field of skin care products, cosmetics.

6. Use of an active composition having a skin aging delaying function according to claim 5, wherein the active composition is contained in an amount of 10 to 30wt% in skin care products, cosmetics.