KR20050004355A - Cosmetics compositions comprising natural materials - Google Patents

Abstract

PURPOSE: Provided is a cosmetics composition comprising the extract of natural materials including Rosa laevigata, Agrimonia pilosa Ledeb. var. japonica Nakai., Cornus officinalis Sieb. et Zucc., and pine tree bark. Therefore, the composition has improving effect on acne and skin wrinkles and skin whitening effect. CONSTITUTION: The functional cosmetic composition for improving acne and skin wrinkles and whitening the skin is characterized by containing the nanosome including at least one natural material selected from Rosa laevigata, Agrimonia pilosa Ledeb. var. japonica Nakai., Cornus officinalis Sieb. et Zucc., and pine tree bark, and NAG(N-acetyl-D-glucosamine). The weight ratio of Rosa laevigata to Agrimonia pilosa Ledeb. var. japonica Nakai. to Cornus officinalis Sieb. et Zucc. to pine tree bark is 0.9-1.1:0.9-1.1:1.3-1.7:0.9-1.1:0.4-0.6. And the weight ratio of the natural material to NAG is 1:0.1-1:20.

Description

Functional cosmetic composition containing a natural raw material {COSMETICS COMPOSITIONS COMPRISING NATURAL MATERIALS}

The present invention relates to a functional cosmetic composition, and more particularly, to a functional cosmetic composition containing an extract extracted according to the supercritical extraction method of natural raw material from golden primrose, Seonhakcho, ume, rhubarb, pine bark, acne, whitening effect In addition, the present invention relates to a cosmetic composition for exhibiting an anti-wrinkle effect, and more particularly, to a cosmetic composition, and more particularly to a cosmetic composition having an acne and whitening and anti-wrinkle effect.

Acne is a sebum-related disease caused by excess sebum caused by male hormones. Specifically, sebum is produced by male hormones and pore keratinization causes sebum to be discharged out of the pores, forming microsurfaces. Acne occurs due to the action of propionibacterium acnes , an anaerobic fungi present.

The skin also contains the Gram-positive strains Staphylococcus aureus , Staphylococcus epidermidis , Streptococcus pyogenes , Mycococcus luteus and Corynebacterium Corynebacterium xerosis , gram-negative strains Escherichia coli and Pseudomonas aeruginosa , yeast Candida albicans and Malassezia furfur and fungus trichophyton Phytis ( Trichophyton Mentagrophytes ) is listed , among them Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes are representative of skin infections, cellulitis, or general inflammation. Strain.

Generally, in order to treat skin diseases such as acne and inflammation, a chemical agent such as vitamin A derivative, triclosan, azelaic acid, or benzolperoxide is applied. Since it can cause a lot of irritation to the skin according to the lesion symptoms, it should be used after consultation with a specialist according to the condition of the skin and acne symptoms.An antimicrobial agent such as tetracycline is used as an antiseptic treatment using antimicrobial agents. . However, these antimicrobial agents have caused many problems, such as causing resistant bacteria, side effects such as photosensitivity. In addition, trichloroacetic acid and glycolic acid, which are used in the skin dermabrasion method to dissolve skin keratin, also have the disadvantage of causing skin sensitization reaction or dissolving keratin, resulting in rough skin. . Therefore, in order to prevent acne and inflammation, it is necessary to develop hypoallergenic cosmetics by using natural antibacterial action or natural derived materials in addition to chemical ingredients that are safe for skin.

In general, a person turns black after being exposed to sunlight. This is a mechanism for protecting the body from ultraviolet rays. That is, upon receiving ultraviolet rays, tyrosinase enzymes are promoted in melanocytes, which are melanin forming cells, to produce and release a black melanin pigment, which is distributed to epidermal cells, thereby blocking the ultraviolet rays. However, excessive formation and deposition of melanin pigments causes appearance problems such as blackening of the skin, blemishes, and freckles. Accordingly, whitening cosmetics are required to prevent their skin symptoms.

There are various kinds of melanin depending on the organism, and it is reported that the melanin is mainly biosynthesized by the action of tyrosinase. Tyrosinase is a metalloproteinase that binds to copper. It is widely distributed in animals, plants, microorganisms, and humans. Tyrosine is used as a dihydroxy phenylalanine (dopa, dihydroxyl-L-phenylalanine, L-DOPA), and dihydrate. It acts to convert oxy phenylalanine into dopaquinone. Dopaquinone is converted to dopachrome, and dopachrome is converted to dihydroxyindole and finally synthesized melanin through oxidative polymerization of dihydrocyindole and binding with protein.

Tyrosinase inhibitors known to date include hydroquinone, hydroxyanisole, ascorbic acid derivatives, kojic acid, azelaic acid and corticosteroids. corticosteroids, retinoids, arbutin, etc., but there are difficulties in use due to safety and economical problems. In particular, hydroxyanisole and hydroquinone have strong melanogenesis inhibitory activity, but at the same time cause side effects such as degeneration or lethality of pigment cells and impaired the original function of the cells. And koji acid has a problem such as discoloration, instability of the material itself during use. Thus, many tyrosinase inhibitors have been developed and are currently used in cosmetics and pharmaceuticals, but their use is limited due to insufficient whitening effects, stability problems on the skin, formulation and stability problems when formulated in cosmetics, and the like.

On the other hand, wrinkles can be said to be the form and structure of the skin that is locally formed and settled on the site that is chronically deformed by muscle movement. Examining the wrinkle formation mechanism together can be said to be important for understanding the actual wrinkle formation mechanism.

In this regard, as skin internal structure changes due to photoaging, epidermis, thickening of the dermis, accumulation of abnormal elastic material, or three-dimensional structure change of elastic fibers have been reported. In addition, for collagen called skeleton, which is a major component of skin structure, microfiberization and weakening of fibrous form of collagen fibers have been revealed (Zheng P. and kligman LH, uvA-induced ultrastructural changes in hairless mouse skin: A comparison to uv B-induced damage., J. Invest.dermatol., 100,194-199 (1993)). However, the collagen fiber bundles formed by these bundles are known to have a great influence on the toughness of the skin through the study of the wound healing process, etc., but the effects on the structural change, the physical properties of the skin and the wrinkle formation due to photoaging There has not been enough review so far.

The mainstream of the recent skin aging research is the study of the direct and indirect effects of sunlight due to the destruction of the ozone layer, and the use of various substances to measure the effect of wrinkle formation caused by the light is actively used. I am researching.

In order to solve the problems as described above, the present invention is excellent in acne prevention, whitening, and anti-wrinkle effect without skin irritation, but also has a safety for the skin and has a natural raw material extract having stability even when combined with cosmetics It is an object to provide a functional cosmetic composition containing.

1 is a view showing the distribution of fluorescent nanoparticles absorbed in the skin cross-section of the cosmetic component according to the present invention.

In order to achieve the object as described above, the present invention is a functional cosmetic composition comprising a natural raw material, at least one natural raw material selected from the group consisting of golden primrose, Seonhakcho, ume, rhubarb and pine bark and NAG (N-acetyl- D-glucosamine), the natural raw material and the NAG provides a functional cosmetic composition, characterized in that stabilized with nanosomes to facilitate skin penetration.

The natural raw materials include golden primrose, Seonhakcho, ume, rhubarb and pine bark, the weight ratio is 0.9 ~ 1.1: 0.9 ~ 1.1: 1.3 ~ 1.7: 0.9 ~ 1.1: 0.4 ~ 0.6, preferably 1: 1 1.5: 1: may be 0.5.

In the cosmetic composition, the natural raw material and the NAG are 1: 0.01 to 1:20, preferably 1: 0.1 to 1:10, respectively, in a weight ratio of 5 to 20% by weight relative to the total weight of the nanosomes. have.

In the cosmetic composition, the nanosomes may be 0.1 to 20% by weight, preferably 0.1 to 10% by weight relative to the total weight of the cosmetic composition.

In the cosmetic composition, the natural raw material may be extracted by a supercritical extraction method.

In the cosmetic composition, the natural raw material, characterized in that extracted by the supercritical gas extraction method, cosmetic composition.

Here, supercritical extraction is a certain substance becomes a fluid state after a certain high pressure and high temperature (critical point), in which only the active ingredient that is beneficial to humans without toxic or residual substances is separated and purified in high purity. Extraction by supercritical fluid uses a supercritical fluid near or above a critical temperature (Tc) and a critical pressure (Pc) as a solvent. The supercritical fluid exhibits the trade-off between gas and liquid properties. The density is close to the density of the liquid, the viscosity is close to the viscosity of the gas, and the diffusion coefficient is about 100 times greater than the diffusion coefficient of the liquid. Therefore, it shows high solubility and fast mass transfer rate. In addition, the solvent power can be freely controlled by simply changing the temperature and pressure or by using a modifier, so that the target can be selectively separated. Carbon dioxide is generally used as a supercritical fluid, which is easy to separate and purify a heat-stable material due to its low critical temperature. On the other hand, since the supercritical fluid solvent can be easily removed from the product to prevent the environmental pollution that is a problem when extracting the organic solvent and the price is low, it can be said to be a very environmentally and economical extraction method. That is, the advantages of this supercritical extraction method are firstly non-toxic to the skin because carbon dioxide, which is harmless to the human body, is used instead of the solvent. (No residual organic solvents and heavy metals) Second, it can convey the freshness of natural plants and herbal medicines as it is processed at low temperatures (extended activity, preventing degeneration due to heat). Third, the extraction process can be simplified to be biochemically stable (oxygen blocking, microbial contamination prevention).

Liposomes are generally made of phospholipids. These phospholipids consist of hydrophobic long hydrocarbon chains and hydrophilic polar groups, and the hydrophobic long hydrocarbons consist of sphingosine derivatives or glycerol derivatives. The other side has ester linkages with choline, ethanol amine, serine, inositol or glycerol. Phospholipids form these regularly arranged structures in the water medium, namely micelles or lamellar structures.

Liposomes are also similar in structure to biological membranes and are being studied as membrane model systems and physiological mediators (R. Sundler. D. Papahadjpovlos, Biochim. Biophys. Acta, 648.743 (1981)). It is mainly used in emulsion-based products (patent application No. 92-2287), such as lotions and creams, in order to facilitate the delivery to the skin by collecting the ingredients to further increase the effect of these active ingredients.

Hereinafter, the present invention will be described in more detail.

The calyx and fruit of the Rosa loevigata Michx (Roseaceae) contain organic acids (apple and lemon acid), tannins, essential oils, vitamin C, resins, and saponins. In the treatment of motion, calyx and berries are toned, convergent, and diarrhea. Frequent oil, urine, and urine with this medicine is used for chronic inflammation and diarrhea.

Outposts of Agrimonia pilosa Ledebour (Roseaceae) include vitamin K, tannins, essential oils, luteolin-7-glucoside, apigenin-7-glucoside, agrimonol, coumarin, and alkaloids in the roots. Coltanine, agrimonoside, ellagic acid, saponin are the leaves have pyrocatechinnin, resins, sterols, ascorbic acid. In the treatment of motion, outposts are used as tonics, blood drops, astringents, and inflammatory drugs in hemostasis, bleeding, bleeding, pissing, crayfish, erythema, and diarrhea.

MUME FRUCTUS is the fruit of the plum tree (Prunus mume sieb.et Zucc. Rose) and is used for embryonic pain, crab and diarrhea.

Roots and root stems of Rheum coreanum Nakai are the anthraquinone derivatives, aloe modin, piscione and chrysophanolanthrone, and the leaves contain rutin, organic acids, ascorbic acid and large amounts of potassium salts. . There is an act of diarrhea, diarrhea and diarrhea.

Pine bark contains up to 16% tannin, anthocyanin, fimaric acid, beta-sitosterol, and glucotanin. Oil-soluble substances include arachidyl alcohol (ecozanol) and tetracozanole.

Geumakja, Seonhakcho, Omae, and Rhubarb used the listed materials of the Korean Pharmacopoeia Herbal Medicine Standard (Korea Food and Drug Administration Notice No. 2003-21), and pine bark was harvested from nature. NAG was purchased from SIGMA Corporation.

Acne prevention and whitening, wrinkle improvement effect was confirmed by the nanosomes containing one or more extracts of the extract extracted by the supercritical extraction method of the above natural raw materials.

In addition, composites A and NAG are mixed in a weight ratio of from 1: 0.01 to 1:20, preferably from 1: 0.1 to 1:10.

The stabilized nanoliposomes are preferably contained in an amount of 0.1 to 20% by weight based on the total weight of the cosmetic composition. If it is less than 0.1% by weight, it is difficult to expect an effective effect, and if it exceeds 20% by weight, there is no obvious increase in efficacy. Therefore, 0.1-20 weight% is preferable and 0.1-10 weight% is more preferable. If the nanosome is added in an amount less than 0.1% by weight based on the total weight, it is difficult to expect the acne suppression effect, the whitening effect and the wrinkle improvement effect. It is both economic and economical.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the present invention, but the content of the present invention is not limited to the following examples.

Example 1

[Production Example 1]

-Golden Pentagram, Seonhakcho, Omae, Rhubarb, Pine Bark Extract Method-

First, 1,3BG (Butylene Glycol) and ethanol are mixed in the same mass ratio and stirred to form a single phase, which is used as a cosolvent to be used in the extraction process using supercritical carbon dioxide. Golden cherry blossoms are dried until there is no change in weight and crushed to a size of 0.3 cm or less using a grinder. After mixing the crushed golden cherry and co-solvent at a mass ratio of about 1: 2 and stirring for about 30 minutes, the golden cherry and co-solvent mixture was put into a supercritical extraction tank having an internal volume of 1.5 L and sealed, and then carbon dioxide was obtained by using a high pressure gas pump. To increase the pressure. The temperature inside the extraction tank is raised to about 40 degrees Celsius at a rate of about 0.5 degrees Celsius per minute and maintained for about 50 minutes. The carbon dioxide was again released while supplying carbon dioxide and decompressed to atmospheric pressure to separate the carbon dioxide and golden primrose extract, and the carbon dioxide was released into the atmosphere, and the golden primrose extract was collected by a collector and used in the present invention.

Sunhakcho, omae, rhubarb and pine bark are put in place of the golden cherry tree to obtain each Seonhakcho, omae, rhubarb and pine bark extract.

[Production Example 2]

Each extract golden preparation of Preparation Example 1 Seonhakcho: Seonhakcho: ume: rhubarb: pine bark in a weight ratio of 1: 1: 1.5: 1: 0.5 by mixing ("Compound A") to nanosomes.

[Production Example 3]

Composite A and NAG were mixed at a weight ratio of 1: 0.5 to nanosomes.

Comparative Example 1

After pulverizing each of the target material (Gold Primrose, Seonhakcho, ume, rhubarb, pine bark) of Preparation Example 1 to a size of 0.3 cm or less by using a pulverizer, the extracts of each of the target substances by the ethanol extraction method compared the present invention Used as target material

Comparative Example 2

Each extract of Comparative Example 1 Golden Primrose: Seonhakcho: omega: rhubarb: pine bark was nanosomes by mixing ("Compound B") in a weight ratio of 1: 1: 1.5: 1: 0.5.

Comparative Example 3

Composite B and NAG were mixed at a weight ratio of 1: 0.5 to nanosomes.

Experimental Example 1

Comparison of Growth Inhibitory Effects on Strains

In order to compare the growth inhibition effect of bacteria with conventional cosmetic ingredients, salicylic acid, lobbie, which are known to have acne and skin inflammation inhibitory effects in the target materials obtained in Preparation Example 2, Comparative Examples 1 and 2, and the conventional cosmetic composition The experiments were conducted on zosome acnes, phycosaccharide antiacne, triclosan, azelaic acid and phytosphingoshine.

Tryptic soy agar (TSA); Difco co.} Was plated on a solid flat medium plate, each bacterium 1 × 10 ⁷ ea / ml, and 20 μl of each sample was dropped in the center and dried naturally. Each sample was diluted 100-fold and used at a concentration of 10 g / l. After natural drying, the culture was performed for 24 hours at 37 degrees Celsius to determine the presence or absence of transparent low-ring.

The results are shown in Table 1 below.

Results of growth inhibition effect on strains No Target substance S. aureus (ATCC 65389) S. epidermis (ATCC 12228) S. pyogens (ATCC 21059) P. acnes (ATCC 6919) Pseudomonas. aeruginosa One Golden Primrose (Comparative Example 1) +/- + - - +/- 2 Seonhakcho (Comparative Example 1) - +/- - +/- - 3 Omae (Comparative Example 1) +/- - +/- - +/- 4 Rhubarb (Comparative Example 1) - +/- +/- +/- +/- 5 Pine Bark (Comparative Example 1) - - +/- - - 6 Salicylic acid + ++ - +/- - 7 Robby Moth + + - + +/- 8 Picosaccharides Antiacne +/- + - + - 9 Triclosan ++ + ++ +++ +/- 10 Azelaic acid - +/- - + - 11 Phytosphingosine + + +/- + - 13 Preparation Example 2 ++ ++++ +++ +++ +++ 15 Comparative Example 2 +/- ++ +/- + +/-

-: Can't produce low ring, +/-: Strain growth after low ring generation, +, ++: Weak low ring, +++, ++++: Strong low ring

As can be seen from the results of Table 1, the conventional raw materials affect some of the five strains, of which triclosan showed the strongest effect. However, according to recent news (Dr. Christopher McNeill, University of Minnesota, USA: 2002-4-17, Health / Yonhap News), exposure to sunlight converts toxic dioxin into sunlight.

On the other hand, when the natural raw material extracted by the ethanol extraction method is treated alone, almost no ring is produced, or even if the ring is produced, it can be seen that the strain grows, Comparative Example 2 mixed with the natural raw material extracted by the ethanol extraction method Even if the low ring is hardly produced or the low ring is generated, but it can be seen that weakly generated. In the case of Preparation Example 2, in which all of the natural raw materials extracted by the supercritical extraction method were mixed and treated, a strong low-ring ring was generated in all five strains.

Therefore, it can be seen that the cosmetic composition according to the present invention has an excellent effect compared to the raw materials used in cosmetics to treat or prevent inflammation and acne caused by dermatophytes. In addition, because of using a natural-derived raw material, unlike the conventional cosmetic ingredients there is no safe skin irritation or side effects.

(Example 2, Comparative Example 4)

Preparation of herbal ingredients and cosmetics containing NAG

According to the composition of the weight ratio of Table 2 using a composition commonly used in the same industry, the oil and water components, the surfactant and the preparation of Example 3 mixture of Example 1, respectively, are mixed and stirred with a homomixer, heated and cooled to uniformity Mixed cosmetics were prepared. In addition, using the Comparative Example 2 to prepare a cosmetic of Comparative Example 4 in the same composition as in Example 2.

Composition (weight ratio) of cosmetic composition Raw material name Example 2 Comparative Example 4 Poly Solate-60 1.5 1.5 Sorbitan stearate 0.5 0.5 Stearic acid 1.0 1.0 Squalane 5.0 5.0 Cerro stearyl alcohol 2.0 2.0 Seryl Octanoate 5.0 5.0 Carbomer 0.15 0.15 Triethanolamine 0.2 0.2 glycerin 5.0 5.0 Preparation Example 3 Mixture 8 Comparative Example 3 Mixture 8 antiseptic Quantity Quantity Purified water Total 100 Total 100

Experimental Example 2

Skin Elasticity Test

60 healthy women (average age 29.5 years old) over 20 years old were divided into 6 groups under the conditions of 22-24 degrees Celsius and 55% relative humidity, and the cosmetic compositions of Example 2 and Comparative Example 4 were each focused for 12 weeks After application (2 times / day), skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The test results are described in Table 3 below as ΔR5 values (R5 (12 weeks) -R5 (0 weeks)), which are average values for each test group of the Cutometer SEM 575. It shows better elasticity than the ground.

In addition, the degree of improvement was calculated by the following formula (1).

% Improvement = (ΔR5 / before application) X 100

Skin elasticity test result (12 weeks) Before application After application Skin elasticity enhancing effect (ΔR5) % Improvement Example 2 0.74 1.16 0.42 56.76 Comparative Example 4 0.71 0.81 0.1 14.08

According to Table 3, when Example 2 containing the mixture of Preparation Example 3 was used, the skin elasticity increased by 42.68% compared to Comparative Example 3. Therefore, it can be seen that the cosmetic containing nanoliposomes stabilized in Preparation Example 3 according to the present invention effectively enhances skin elasticity than when it is not stabilized with nanoliposomes or using components of NMF (Natural Moisturizing Factor). have. NAG is used to improve the wrinkles of cosmetics to prevent wrinkles, but it can be seen that the combination of the extract with the supercritical extraction method and NAG has a synergistic effect over the additive effect of the two components.

Experimental Example 3

Skin moisture measurement

Seventy healthy women (mean age 29.5 years) were divided into seven groups of 70 healthy women (22 to 24 degrees Celsius, 55% relative humidity and no air flow). The cosmetics of Example 2 and Comparative Example 4 were applied twice a day for 6 weeks to the entire face around the eyes of each group. By using Skicon-200 (IBS Co. Japan), the moisture content of the cheek area was measured before and after application to measure the persistence of the moisturizing effect. The results are shown in Table 4 as an average value for each test group.

The degree of improvement was calculated according to the following equation.

Skin moisture measurement result Before the test 6 weeks after application Improvement (%) Example 2 630 ± 10 890 ± 10 41.27 Comparative Example 4 630 ± 10 635 ± 10 0.78

According to the table, it can be seen that the use of Example 2 stabilized in Preparation Example 3 has a greater effect of improving the skin moisture content compared to Comparative Example 4. At this time, as a result of experiments with various combination ratios of nanoliposomes of Preparation Example 3, it was preferable to use nanoliposomes of 0.1 to 20% by weight. More preferably, 5 to 20% by weight relative to the total weight of the nanoliposome is good, and if it exceeds 20% by weight, there is a problem in the stability of nanosomes and it is uneconomical. It can be seen that there is a synergistic effect beyond the additive effect of.

Experimental Example 4

Human patch experiment

In order to confirm that the cosmetic composition containing a natural substance has no skin irritation and other side effects, a patch test was performed on the human body in the cream formulation of Example 2 and Comparative Example 4.

The test consists of 30 healthy males and females who use a Finn chamber on the back to put cosmetic products directly into aluminum discs or apply 40 μl of paper discs to the skin using a scanpore tape. It fixed, the patch was removed after 24 hours, and the result was determined after 4 hours.

Results are expressed numerically according to the severity of erythema and edema. 1984; Aberer et al, 1993).

The criteria for evaluation are as follows.

0: no redness

1: erythema

2: redness and papules

3: redness, papules and vesicles

4: severe edema and vesicles

The stimulus degree was calculated according to Equation 3 below based on the score determined according to the criterion, and the results are shown in Table 5 below.

Stimulus (%) = (responsiveness of positive response / response of self-population) X 100

Stimulus result Comparative Example 4 Example 2 Irritation degree (%) 5.0 0.02

As can be seen from the results in Table 5, in Example 2, there was no dermatitis or other irritation.

Experimental Example 5

Clinical Trials of Natural Cosmetic Ingredients

Example 2 and Comparative Example 4 were used to conduct animal clinical effects experiments on acne and general inflammation.

After two compartments of 10 hairless mice (hereinafter referred to as "experimental animals") (6 weeks of age, females), arachidonic acid (Sigma Co., Ltd.) induces inflammation and observed with the naked eye. Acquisition and inflammation-induced animal models were constructed by cumulative application of 200 μl of four strains 2 × 10 ⁵ ea at intervals of 2-3 days.

The sample of Example 2 was applied to the left of the constituent model and the sample of Comparative Example 4 to the right was applied at 2 mg / cm 2 daily. When acne and inflammation were induced, the abscess was collected from the affected area and cultured to identify the same strain.

While observing daily by applying the cream formulation of the cosmetic formulation of Example 2 on the left side and the sample of Comparative Example 4 on the right side at 2 mg / cm 2 daily for 2 months to the affected area caused by the same strain as used in the present invention The effect was measured.

As for the determination of the effect of this clinical trial, a standard was set as shown in Table 6 below, and two or three observers determined a score or grade for the criterion and performed relative comparison.

Clinical trial effect criterion Affected state effect One Healing normally Great 2 The affected area is intermediate or no longer in progress usually 3 The affected area is larger or more inflamed none

According to the criteria of Table 6, the results of the visual judgment on the affected part of the experimental animals after one month are shown in Table 7 as the number of animal models.

Clinical Trial Results Formulation / Effect Excellent effect Effect no effect system Example 2 8 2 - 10 Comparative Example 4 - One 9 10

As can be seen from the results of Table 7, 80% of 10 experimental animals showed an excellent effect in the formulation of Example 2, 90% of Comparative Example 4 was found to be ineffective.

Therefore, as a result of clinical experiments on the affected areas of acne and inflammation, it can be seen that the acne and inflammation-releasing effect is excellent in the cosmetic composition to which the natural raw material according to the present invention is added.

Experimental Example 7

Measurement of Transdermal Absorption by Size of Nanoparticles

Nanoparticles having sizes of 50 nm, 80 nm, 120 nm and 500 nm were prepared using PMMA polymers to which fluorescent substances were added by chemical bonding, and then applied to the skin of female hairless guinea pigs. The cross section was cut to measure the fluorescence emitted from the percutaneously absorbed nanoparticles.

Fluorescent PMMA polymer nanoparticles were prepared by covalently bonding fluorescent molecules to a carboxyl group of methacrylic acid of polymethylmethacrylate-co-methacrylic acid (molar ratio of methylmethacrylate and methacrylic acid is 84:16).

The manufacturing method will be described in detail as follows.

34 g of polymethylmethacrylate-co-methacrylic acid was dissolved in 150 ml of anhydrous methylene chloride, and then 132 mg of dicyclohexylcarbodiimide and 74 mg of N-hydroxysuccinimide were added to activate the carboxyl group. After stirring for 1 hour, 222 mg of fluoresceamine was added to react the primary amine of the fluorescein amine with the activated carboxyl group to form an amide bond, thereby allowing the fluorescent molecules to be covalently bonded to the polymer chain. The reaction proceeded for 5 hours at room temperature and dark conditions. Dicyclohexyl urea, a by-product formed after the reaction, was removed through a 0.45 μm nylon filter and then precipitated in diethyl ether to remove the reaction reagents and unreacted residues. Subsequently, it was left in excess tertiary distilled water for one day to remove the remaining residues, and then dried using a vacuum oven at 40 degrees. As a result, PMMA nanoparticles having sizes of 50 nm, 80 nm, 120 nm, and 500 nm to which fluorescent materials were added were prepared.

Eight-week-old female hairless guinea pigs (strain IAF / HA-hrBR) were used for the nanoparticle transdermal absorption test by size. The skin of the abdomen of the guinea pig was cut and mounted in a Franz-type diffusion cell (Lab Fine Instruments, Korea). A 50 mM phosphate buffer (pH 7.4, 0.1 M NaCl) was added to the receiving vessel (5 ml) of the plasma-type diffusion cell. The diffusion cell was mixed and dispersed at 600 rpm while maintaining 32 degrees Celsius, and 50 μl of a 10% (w / v) solution of fluorescent PMMA nanoparticles prepared for each size was added to each donor container. Absorption and diffusion were carried out according to a predetermined time (average 12 hours), and the area of skin where absorption and diffusion occurred was 0.64 cm ² . After the absorption diffusion of nanoparticles was finished, the non-absorbed nanoparticles remaining on the skin were washed with dried Kimwipes or ethanol 10ml, and the cross-section of the skin was cut to measure the distribution of fluorescent PMMA nanoparticles absorbed on the skin section. .

Intra-dermal distribution measurement results of the nanoparticles are shown in Table 8, and the photograph is shown in FIG.

Measurement result of skin distribution of nanoparticles Average size of nanoparticles Percutaneous absorption area 50 nm 35 μm 80 nm 17 μm 120 nm 12㎛ 500 nm 7㎛

In Table 8, it can be seen that the percutaneous absorption of the nanoparticles largely depends on the average particle diameter. Nanoparticles with an average diameter of about 50 nm were passed through the epidermis and absorbed and diffused to the top of the dermal layer. When the particle size exceeded 500 nm, the nanoparticles remained on the skin surface and no longer absorbed into the skin. Did not happen. Nanoparticles with an average diameter of about 50 nm are diffused into the intercellular lipids of the skin, and the hydrophobicity of the polymer is thought to have an effect of promoting such transdermal absorption. Therefore, when the active ingredient is poorly or unstable, and the active ingredient is trapped in the nanoparticles with an average particle diameter of about 50 nm, it was found that the percutaneous absorption of the active ingredient is possible through delivery into the skin through the nanoparticles.

As described above, the functional cosmetic composition comprising the natural raw material according to the present invention inhibits the growth of the microorganisms and bacteria that are involved in acne and inflammation caused by skin on the skin, as well as inhibit the skin inflammation and acne induction, as well as acne and inflammation relief It provides an excellent effect, enhances the elasticity of the skin, improves the moisturizing effect, there is no skin irritation and other side effects, and provides a cosmetic composition capable of transdermal absorption delivery.

Claims (5)

In the functional cosmetic composition containing a natural raw material, The functional cosmetic composition comprises at least one natural raw material selected from the group consisting of golden primrose, Seonhakcho, ume, rhubarb and pine bark and NAG (N-acetyl-D-glucosamine), the natural raw material and the NAG penetrates the skin Functional cosmetic composition, characterized in that stabilized with nanosomes to facilitate The method of claim 1, The natural raw materials include golden primrose, Seonhakcho, five plums, rhubarb and pine bark, the weight ratio of each of 0.9 ~ 1.1: 0.9 ~ 1.1: 1.3 ~ 1.7: 0.9 ~ 1.1: 0.4 ~ 0.6, preferably 1: 1 : 1.5: 1: 0.5, the functional cosmetic composition characterized in that. The method according to claim 1 or 2, The natural raw material and the NAG are 1: 0.01 to 1:20 in weight ratio, respectively, preferably 1: 0.1 to 1:10, characterized in that the functional cosmetic composition. The method according to claim 1 or 2, The nanosomes are functional cosmetic composition, characterized in that 0.1 to 20% by weight, preferably 0.1 to 10% by weight relative to the total weight of the cosmetic composition. The method according to claim 1 or 2, The natural raw material is characterized in that extracted by the supercritical gas extraction method, functional cosmetic composition.