CN111961143B - 一种制备琼脂糖的方法 - Google Patents
一种制备琼脂糖的方法 Download PDFInfo
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- CN111961143B CN111961143B CN202010986900.3A CN202010986900A CN111961143B CN 111961143 B CN111961143 B CN 111961143B CN 202010986900 A CN202010986900 A CN 202010986900A CN 111961143 B CN111961143 B CN 111961143B
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- agarose
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- gracilaria verrucosa
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 81
- 241000703939 Gracilariopsis longissima Species 0.000 claims abstract description 57
- 239000003513 alkali Substances 0.000 claims abstract description 36
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- 239000000084 colloidal system Substances 0.000 claims abstract description 23
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- VANNPISTIUFMLH-UHFFFAOYSA-N glutaric anhydride Chemical compound O=C1CCCC(=O)O1 VANNPISTIUFMLH-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000001914 filtration Methods 0.000 claims abstract description 19
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- 230000007935 neutral effect Effects 0.000 claims description 30
- 238000002791 soaking Methods 0.000 claims description 30
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 20
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 18
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- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 9
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 9
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- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 9
- 235000006408 oxalic acid Nutrition 0.000 claims description 9
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 9
- 239000000499 gel Substances 0.000 abstract description 61
- 229920001817 Agar Polymers 0.000 abstract description 39
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- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract description 20
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 229910001626 barium chloride Inorganic materials 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000000246 agarose gel electrophoresis Methods 0.000 description 5
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- 238000007873 sieving Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 3
- 239000007982 barbital buffer Substances 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
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- 229910052717 sulfur Inorganic materials 0.000 description 3
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- 241001474374 Blennius Species 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
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- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
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- 229920001282 polysaccharide Polymers 0.000 description 2
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- 150000004804 polysaccharides Chemical class 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
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- 229920002307 Dextran Polymers 0.000 description 1
- 241000206581 Gracilaria Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
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- 239000003957 anion exchange resin Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940125717 barbiturate Drugs 0.000 description 1
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 description 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012527 feed solution Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
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- 239000002808 molecular sieve Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002133 sample digestion Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
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CN202010986900.3A CN111961143B (zh) | 2020-09-18 | 2020-09-18 | 一种制备琼脂糖的方法 |
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CN111961143B true CN111961143B (zh) | 2022-09-30 |
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CN115612185A (zh) * | 2022-10-09 | 2023-01-17 | 集美大学 | 一种大孔刚性琼脂糖凝胶微球的制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010109289A1 (en) * | 2009-03-24 | 2010-09-30 | Council Of Scientific & Industrial Research | Process for the preparation of agarose polymer from seaweed extractive |
CN110003361A (zh) * | 2019-04-23 | 2019-07-12 | 绿新(福建)食品有限公司 | 一种高品质琼脂的制备方法 |
CN111499774A (zh) * | 2020-06-16 | 2020-08-07 | 集美大学 | 一种基于数学模型的江蓠琼脂生产方法 |
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2020
- 2020-09-18 CN CN202010986900.3A patent/CN111961143B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010109289A1 (en) * | 2009-03-24 | 2010-09-30 | Council Of Scientific & Industrial Research | Process for the preparation of agarose polymer from seaweed extractive |
CN110003361A (zh) * | 2019-04-23 | 2019-07-12 | 绿新(福建)食品有限公司 | 一种高品质琼脂的制备方法 |
CN111499774A (zh) * | 2020-06-16 | 2020-08-07 | 集美大学 | 一种基于数学模型的江蓠琼脂生产方法 |
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Effective date of registration: 20221227 Address after: No.28, Lane 908, Ziping Road, Pudong New Area, Shanghai, 201321 Patentee after: Shanghai Aopu Mai Biotechnology Co.,Ltd. Address before: No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, 361000 Patentee before: JIMEI University |
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Effective date of registration: 20240107 Address after: Floor 1-2, Building No. 2, 500 Lane, Furong Hualu, Pudong New Area, Shanghai, 201321 Patentee after: Shanghai Aoruichun Biotechnology Co.,Ltd. Address before: No.28, Lane 908, Ziping Road, Pudong New Area, Shanghai, 201321 Patentee before: Shanghai Aopu Mai Biotechnology Co.,Ltd. |
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