CN111995699B - 一种制备琼脂糖的方法 - Google Patents
一种制备琼脂糖的方法 Download PDFInfo
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- CN111995699B CN111995699B CN202010986850.9A CN202010986850A CN111995699B CN 111995699 B CN111995699 B CN 111995699B CN 202010986850 A CN202010986850 A CN 202010986850A CN 111995699 B CN111995699 B CN 111995699B
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- 238000000034 method Methods 0.000 title claims abstract description 45
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- 241000703939 Gracilariopsis longissima Species 0.000 claims abstract description 53
- 238000005406 washing Methods 0.000 claims abstract description 42
- 239000003513 alkali Substances 0.000 claims abstract description 41
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 21
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- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 30
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- 229920001817 Agar Polymers 0.000 abstract description 35
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000003712 decolorant Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
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- 229910052717 sulfur Inorganic materials 0.000 description 3
- 239000011593 sulfur Substances 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- 241001474374 Blennius Species 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 239000007982 barbital buffer Substances 0.000 description 2
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- 239000003086 colorant Substances 0.000 description 2
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- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
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- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
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- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 150000004804 polysaccharides Chemical class 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- DCQFFOLNJVGHLW-DSOBHZJASA-N 3,6-anhydro-alpha-L-galactopyranose Chemical compound O[C@H]1[C@@]2([H])OC[C@]1([H])O[C@@H](O)[C@H]2O DCQFFOLNJVGHLW-DSOBHZJASA-N 0.000 description 1
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000206581 Gracilaria Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229920001284 acidic polysaccharide Polymers 0.000 description 1
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- 239000003957 anion exchange resin Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- 229940125717 barbiturate Drugs 0.000 description 1
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 description 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 1
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- WZYRMLAWNVOIEX-UHFFFAOYSA-N cinnamtannin B-2 Natural products O=CC(O)C1OCC(O)C1O WZYRMLAWNVOIEX-UHFFFAOYSA-N 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002133 sample digestion Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
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Priority Applications (1)
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CN202010986850.9A CN111995699B (zh) | 2020-09-18 | 2020-09-18 | 一种制备琼脂糖的方法 |
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CN202010986850.9A CN111995699B (zh) | 2020-09-18 | 2020-09-18 | 一种制备琼脂糖的方法 |
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CN111995699A CN111995699A (zh) | 2020-11-27 |
CN111995699B true CN111995699B (zh) | 2022-07-19 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010109289A1 (en) * | 2009-03-24 | 2010-09-30 | Council Of Scientific & Industrial Research | Process for the preparation of agarose polymer from seaweed extractive |
CN109180837A (zh) * | 2018-07-05 | 2019-01-11 | 华侨大学 | 一种调节琼脂糖凝胶透明度的方法 |
CN111499774A (zh) * | 2020-06-16 | 2020-08-07 | 集美大学 | 一种基于数学模型的江蓠琼脂生产方法 |
-
2020
- 2020-09-18 CN CN202010986850.9A patent/CN111995699B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010109289A1 (en) * | 2009-03-24 | 2010-09-30 | Council Of Scientific & Industrial Research | Process for the preparation of agarose polymer from seaweed extractive |
CN109180837A (zh) * | 2018-07-05 | 2019-01-11 | 华侨大学 | 一种调节琼脂糖凝胶透明度的方法 |
CN111499774A (zh) * | 2020-06-16 | 2020-08-07 | 集美大学 | 一种基于数学模型的江蓠琼脂生产方法 |
Non-Patent Citations (3)
Title |
---|
"江蓠琼脂提取与顺丁烯二酸酐酯化琼脂制备工艺及其应用";张佳斌;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20190815(第08期);第10-11、30-31、63页 * |
"琼胶的预冷和冻结效果试验";李来好;《制冷》;19891231(第4期);第9-10页 * |
"酶法替代碱法提取琼脂生产技术开发";翁惠芬等;《集美大学学报( 自然科学版)》;20180128;第23卷(第1期);第17-26页 * |
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Effective date of registration: 20221223 Address after: No.28, Lane 908, Ziping Road, Pudong New Area, Shanghai, 201321 Patentee after: Shanghai Aopu Mai Biotechnology Co.,Ltd. Address before: No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, 361000 Patentee before: JIMEI University |
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Effective date of registration: 20231225 Address after: Floor 1-2, Building No. 2, 500 Lane, Furong Hualu, Pudong New Area, Shanghai, 201321 Patentee after: Shanghai Aoruichun Biotechnology Co.,Ltd. Address before: No.28, Lane 908, Ziping Road, Pudong New Area, Shanghai, 201321 Patentee before: Shanghai Aopu Mai Biotechnology Co.,Ltd. |
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