CN111868242A - 能从无细胞合成中获得的封闭端DNA载体和用于获得ceDNA载体的方法 - Google Patents

能从无细胞合成中获得的封闭端DNA载体和用于获得ceDNA载体的方法 Download PDF

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CN111868242A
CN111868242A CN201980019414.XA CN201980019414A CN111868242A CN 111868242 A CN111868242 A CN 111868242A CN 201980019414 A CN201980019414 A CN 201980019414A CN 111868242 A CN111868242 A CN 111868242A
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itr
sequence
sense
expression cassette
stranded
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O·阿尔坎
R·M·科廷
M·斯坦顿
D·A·科尔
C·佩尔蒂埃
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Generational Biology Co
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore
    • C—CHEMISTRY; METALLURGY
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/103—Plasmid DNA for invertebrates
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    • C12N2820/60—Vectors comprising a special origin of replication system from viruses
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE

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CN201980019414.XA 2018-01-19 2019-01-18 能从无细胞合成中获得的封闭端DNA载体和用于获得ceDNA载体的方法 Pending CN111868242A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862619392P 2018-01-19 2018-01-19
US62/619,392 2018-01-19
PCT/US2019/014122 WO2019143885A1 (en) 2018-01-19 2019-01-18 Closed-ended dna vectors obtainable from cell-free synthesis and process for obtaining cedna vectors

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CN111868242A true CN111868242A (zh) 2020-10-30

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US (1) US12442015B2 (https=)
EP (1) EP3740571A4 (https=)
JP (3) JP2021511047A (https=)
KR (2) KR20250159738A (https=)
CN (1) CN111868242A (https=)
AU (1) AU2019210034B2 (https=)
BR (1) BR112020013319A2 (https=)
CA (1) CA3088984A1 (https=)
IL (1) IL275878B2 (https=)
MA (1) MA51626A (https=)
MX (1) MX2020005790A (https=)
MY (1) MY208589A (https=)
PH (1) PH12020550878A1 (https=)
SG (1) SG11202005271TA (https=)
UA (1) UA130154C2 (https=)
WO (1) WO2019143885A1 (https=)

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CA3147414A1 (en) 2019-07-17 2021-01-21 Generation Bio Co. Synthetic production of single-stranded adeno associated viral dna vectors
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US20230138409A1 (en) * 2020-03-24 2023-05-04 Generation Bio Co. Non-viral dna vectors and uses thereof for expressing factor ix therapeutics
JP2023535632A (ja) 2020-07-27 2023-08-18 アンジャリウム バイオサイエンシズ エージー Ｄｎａ分子の組成物、その作製方法、及びその使用方法
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EP4214309A4 (en) * 2020-09-16 2025-07-16 Generation Bio Co CLOSED-END DNA VECTORS AND RELATED USES FOR EXPRESSING PHENYLALANINE HYDROXYLASE (PAH)
GB202014751D0 (en) 2020-09-18 2020-11-04 Lightbio Ltd Targeting vector
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US20240100132A1 (en) * 2021-01-25 2024-03-28 Prevail Therapeutics, Inc. Modulation of aav-based gene expression
JP2024517427A (ja) 2021-04-20 2024-04-22 アンジャリウム バイオサイエンシズ エージー アミロ－α－１，６－グルコシダーゼ、４－α－グルカノトランスフェラーゼをコードするＤＮＡ分子の組成物、その作製の方法、及びその使用の方法
US20240216535A1 (en) * 2021-04-27 2024-07-04 Generation Bio Co. Non-viral dna vectors expressing anti-coronavirus antibodies and uses thereof
AU2022264509A1 (en) * 2021-04-27 2023-12-14 Generation Bio Co. Non-viral dna vectors expressing therapeutic antibodies and uses thereof
KR20240005903A (ko) * 2021-05-07 2024-01-12 제너레이션 바이오 컴퍼니 백신 전달을 위한 비바이러스성 dna 벡터
CA3218126A1 (en) * 2021-05-07 2022-11-10 Matthew MANGANIELLO Lyophilized non-viral dna vector compositions and uses thereof
AU2022422134A1 (en) * 2021-12-23 2024-07-04 Generation Bio Co. Scalable and high-purity cell-free synthesis of closed-ended dna vectors
JP2025504404A (ja) 2022-01-14 2025-02-12 アンジャリウム バイオサイエンシズ エージー Ｖｉｉｉ因子をコードするｄｎａ分子の組成物、それを作製する方法、及びその使用方法
GB202204112D0 (en) 2022-03-23 2022-05-04 Lightbio Ltd Linear construct
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KR20250129818A (ko) * 2022-12-01 2025-08-29 제너레이션 바이오 컴퍼니 합성 단일-가닥 dna 분자 및 이를 생산하고 사용하는 방법
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UA130154C2 (uk) 2025-12-03
MY208589A (en) 2025-05-19
EP3740571A4 (en) 2021-12-08
PH12020550878A1 (en) 2021-04-05
KR102879598B1 (ko) 2025-11-03
RU2020127017A (ru) 2022-02-21
MA51626A (fr) 2020-11-25
JP2025113389A (ja) 2025-08-01
BR112020013319A2 (pt) 2020-12-01
US20210071197A1 (en) 2021-03-11
IL275878B2 (en) 2026-03-01
IL275878B1 (en) 2025-11-01
WO2019143885A1 (en) 2019-07-25
KR20200111726A (ko) 2020-09-29
MX2020005790A (es) 2020-10-28
EP3740571A1 (en) 2020-11-25
JP2023126487A (ja) 2023-09-07
JP2021511047A (ja) 2021-05-06
IL275878A (en) 2020-08-31
KR20250159738A (ko) 2025-11-11
AU2019210034A1 (en) 2020-07-09
US12442015B2 (en) 2025-10-14
AU2019210034B2 (en) 2025-03-27
SG11202005271TA (en) 2020-07-29
CA3088984A1 (en) 2019-07-25

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