CN111819952A - Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes - Google Patents
Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes Download PDFInfo
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- CN111819952A CN111819952A CN202010746068.XA CN202010746068A CN111819952A CN 111819952 A CN111819952 A CN 111819952A CN 202010746068 A CN202010746068 A CN 202010746068A CN 111819952 A CN111819952 A CN 111819952A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/10—Fertilisers containing plant vitamins or hormones
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention discloses a method for embedding, sprouting and germinating miniature potatoes in Huaiyishan mountains, which belongs to the field of agricultural science, and the method comprises the steps of soaking the miniature potatoes in MS culture solution containing different components, mixing the miniature potatoes with improved MS culture solution, embedding, and culturing under special conditions to germinate the miniature potatoes; according to the method, the germination of the minipotatoes of the Huaiyoshan mountain can be effectively promoted through soaking and embedding culture, the germination rate of the minipotatoes of the Huaiyoshan mountain is greatly improved, and the germination of the embedded beads of the minipotatoes of the Huaiyoshan mountain is promoted to grow into seedlings by combining MS culture solution irrigation and day-night temperature difference alternate culture, so that the germination rate of the embedded beads of the minipotatoes of the Huaiyoshan mountain is effectively improved, the plants are promoted to be robust, sufficient nutrients can be provided, and the method is favorable for subsequent culture and transplanting survival.
Description
Technical Field
The invention relates to the field of agricultural science, in particular to a method for embedding, accelerating germination and sprouting of miniature Huaiyoshan high-mountain potatoes.
Background
The potato miniature potato is a miniature potato block with the diameter of 1-10 cm produced by transplanting virus-free test-tube seedlings or cutting stem segments in an artificially prepared nutrient medium in an insect-proof greenhouse. The miniature potato factory production is a new technology for producing potato virus-free seed potatoes in a large scale and high standard by combining a soilless culture technology, a plant tissue culture technology, an aeroponic culture technology and a cuttage rapid propagation technology. Because the miniature potatoes have small volume and weight of only a few grams, the miniature potatoes are convenient to transport in large quantities, and the problem of difficult potato seed mixing and transportation is solved. Therefore, the production and development of the miniature potatoes are fast, and the miniature potatoes become one of the main technical measures for detoxification and rapid propagation in China.
However, the potato micro-potatoes have small volume, light weight, low content of nutrient substances and energy substances such as starch and the like, weak growth vigor, low emergence rate, easy water evaporation and loss during storage, often influence germination and emergence, and even influence seed reproduction and yield due to reduced vitality of the potato seeds, and the problems become main obstacles for developing potato seed production. Therefore, how to maintain or improve the vitality of the miniature seed potatoes and solve the problem that the miniature seed potatoes have low germination rate and even do not germinate becomes an important subject of domestic and foreign research.
Disclosure of Invention
The invention aims to provide a method for embedding, accelerating germination and germinating miniature potatoes in the mountains of Huaiyoshan so as to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for embedding, accelerating germination and germination of miniature potatoes in mountains and mountains of Huaiyan mountains, which comprises the following steps:
soaking potato microtubers in a first MS culture solution, a second MS culture solution, a third MS culture solution and a fourth MS culture solution respectively; the first MS culture solution contains gibberellin, the second MS culture solution contains chitosan oligosaccharide, the third MS culture solution contains graphene quantum dots, and the fourth MS culture solution contains carbon nano tubes;
mixing the soaked potato micro potatoes with an improved MS culture solution to obtain a micro potato mixture; the improved MS culture solution contains sodium alginate and sucrose but does not contain calcium ions;
dripping the miniature potato mixture into an embedding MS culture solution for embedding to obtain potato miniature potato embedded beads;
planting the potato miniature embedded beads, and alternately culturing under different culture conditions; the culture conditions are respectively illumination culture and dark culture, and the temperature of the illumination culture is higher than that of the dark culture;
and alternately culturing until the potato micro potato embedding beads germinate and emerge.
Further, the soaking temperature of the potato micro-potatoes is 24-26 ℃.
Further, the first MS culture solution contains 0.05g/L of gibberellin, the second MS culture solution contains 0.05g/L of chitosan oligosaccharide, the third MS culture solution contains 0.3-0.5g/L of graphene quantum dots, and the fourth MS culture solution contains 0.3-0.5g/L of carbon nanotubes.
Further, the concentration of sodium alginate in the modified MS culture solution is 0.03g/L, and the concentration of sucrose is 0.2 mol/L.
Furthermore, the embedded MS culture solution also contains 0.05g/L gibberellin, 0.05g/L chitosan oligosaccharide, 0.03-0.05g/L graphene quantum dots, 0.03-0.05g/L carbon nano tubes and 0.1mol/LCaCl2And 0.2mol/L sucrose.
Further, the embedding temperature is 24-26 ℃.
Further, the temperature of the light culture is 34-36 ℃, the light intensity is 1500-.
Further, the time of the light culture is 12h/d, and the time of the dark culture is 12 h/d.
The invention discloses the following technical effects:
through embedding, form the miniature potato of the high mountain sweet potato of huaiyushan and bury the pearl, can effectively exert the effect that the dormancy was broken to gibberellin, chitosan oligosaccharide can further promote the effect that the dormancy was broken to gibberellin, and graphite alkene quantum dot and carbon nanotube then adsorbable moisture and nutrient, reinforcing gibberellin breaks the miniature potato of the high mountain sweet potato of huaiyushan ability, improves the germination rate and the germination power of the miniature potato of the high mountain sweet potato of huaiyushan.
According to the method, the germination of the Huaiyoshan mountain high-mountain potato miniature potatoes can be effectively promoted through soaking and embedding culture, the germination rate is greatly improved, and meanwhile, the seedlings are promoted by combining MS culture solution for irrigation, so that the germinated Huaiyoshan mountain high-mountain potato miniature potatoes can grow into seedlings through embedding beads, sufficient nutrients can be provided, and the subsequent culture and transplanting survival are facilitated.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
In the present example, miniature potatoes of Huaiyoshan mountain potatoes of about 0.5cm in size were used.
Example 1
A method for embedding, accelerating germination and germinating miniature potatoes in Huaiyoshan mountains comprises the following steps:
(1) soaking miniature rhizoma Solani Tuber osi at 25 deg.C in MS culture solution containing 0.05g/L gibberellin for 24 hr;
(2) soaking miniature rhizoma Dioscoreae potato in MS culture solution containing 0.05g/L chitosan oligosaccharide at 25 deg.C for 24 hr;
(3) soaking miniature Moyushan high-mountain potato potatoes in an MS culture solution containing 0.4g/L of graphene quantum dots at 25 ℃ for 24 hours;
(4) soaking miniature rhizoma Solani Tuber osi in Moyushan mountain at 25 deg.C in MS culture solution containing 0.4g/L carbon nanotube for 24 hr;
(5) mixing soaked potato micro potato with improved MS culture solution containing 0.03g/L sodium alginate and 0.2mol/L sucrose at 25 deg.C to obtain micro potato mixture, and dripping the micro potato mixture into solution containing 0.05g/L gibberellin, 0.05g/L chitosan oligosaccharide, 0.04g/L graphene quantum dot, 0.04g/L carbon nanotube, and 0.1mol/L LCaCl2Embedding the mixture in an embedding MS culture solution of 0.2mol/L sucrose;
(6) after embedding, planting the miniature Huaiyshan high-mountain potato embedded beads in a nutrition pot, wherein the nutrition pot contains perlite, vermiculite and peat in a mass ratio of 1:1:2, irrigating MS culture solution in the morning and evening, alternately culturing at 35 ℃ and 20 ℃ every day, and performing illumination for 12h/d at 35 ℃, light intensity of about 1800lx and illumination for 12h/d at 20 ℃ in the dark;
(7) the embedding beads of the Huaiyoshan high-mountain potatoes are alternately cultured for about 7 days, and the Huaiyoshan high-mountain potatoes integrally germinate and emerge, so that the Huaiyoshan high-mountain potatoes can be used for subsequent transplantation.
Example 2
A method for embedding, accelerating germination and germinating miniature potatoes in Huaiyoshan mountains comprises the following steps:
(1) soaking miniature rhizoma Solani Tuber osi at 24 deg.C in MS culture solution containing 0.05g/L gibberellin for 24 hr;
(2) soaking miniature sweet potato in MS culture solution containing chitosan oligosaccharide 0.05g/L at 26 deg.C for 24 hr;
(3) soaking miniature Moyushan high-mountain potato potatoes in an MS culture solution containing 0.5g/L of graphene quantum dots at 24 ℃ for 24 hours;
(4) soaking miniature sweet potato in MS culture solution containing 0.3g/L carbon nanotube at 26 deg.C for 24 hr;
(5) mixing soaked potato micro potato with improved MS culture solution containing 0.03g/L sodium alginate and 0.2mol/L sucrose at 26 deg.C to obtain micro potato mixture, and dripping the micro potato mixture into solution containing 0.05g/L gibberellin, 0.05g/L chitosan oligosaccharide, 0.03g/L graphene quantum dot, 0.05g/L carbon nanotube, and 0.1mol/L LCaCl2Embedding the mixture in an embedding MS culture solution of 0.2mol/L sucrose;
(6) after embedding, planting the miniature Huaiyshan high-mountain potato embedded beads in a nutrition pot, wherein the nutrition pot contains perlite, vermiculite and peat in a mass ratio of 1:1:2, irrigating MS culture solution in the morning and evening, alternately culturing at 34 ℃ and 21 ℃ every day, and performing illumination for 12h/d at 34 ℃, light intensity of about 1500lx and illumination for 12h/d at 21 ℃ in the dark;
(7) the embedding beads of the Huaiyoshan high-mountain potatoes are alternately cultured for about 7 days, and the Huaiyoshan high-mountain potatoes integrally germinate and emerge, so that the Huaiyoshan high-mountain potatoes can be used for subsequent transplantation.
Example 3
A method for embedding, accelerating germination and germinating miniature potatoes in Huaiyoshan mountains comprises the following steps:
(1) soaking miniature potato in MS culture solution containing 0.05g/L gibberellin at 26 deg.C for 24 hr;
(2) soaking miniature rhizoma Dioscoreae potato in MS culture solution containing 0.05g/L chitosan oligosaccharide at 24 deg.C for 24 hr;
(3) soaking miniature potatoes in Moyushan mountain potato culture solution containing 0.3g/L of graphene quantum dots at 26 ℃ for 24 hours;
(4) soaking miniature rhizoma Solani Tuber osi in Moyushan mountain at 24 deg.C in MS culture solution containing 0.5g/L carbon nanotube for 24 hr;
(5) mixing soaked potato micro potato with improved MS culture solution containing 0.03g/L sodium alginate and 0.2mol/L sucrose at 24 deg.C to obtain micro potato mixture, and dripping the micro potato mixture into solution containing 0.05g/L gibberellin, 0.05g/L chitosan oligosaccharide, 0.05g/L graphene quantum dot, 0.03g/L carbon nanotube, and 0.1mol/L LCaCl2Embedding the mixture in an embedding MS culture solution of 0.2mol/L sucrose;
(6) after embedding, planting the miniature Huaiyshan high-mountain potato embedded beads in a nutrition pot, wherein the nutrition pot contains perlite, vermiculite and peat in a mass ratio of 1:1:2, irrigating MS culture solution in the morning and evening, alternately culturing at 36 ℃ and 19 ℃ every day, and performing illumination for 12h/d at 36 ℃, light intensity of about 2000lx and illumination for 12h/d at 19 ℃ in the dark;
(7) the embedding beads of the Huaiyoshan high-mountain potatoes are alternately cultured for about 7 days, and the Huaiyoshan high-mountain potatoes integrally germinate and emerge, so that the Huaiyoshan high-mountain potatoes can be used for subsequent transplantation.
Comparative example 1
The difference from example 1 is that the soaking temperature in steps (1) to (4) of comparative example 1 is 23 ℃.
Comparative example 2
The difference from example 1 is that the soaking solutions of steps (1) to (4) of comparative example 2 were all MS culture solutions without adding other components.
Comparative example 3
The difference from example 1 is that the embedded MS culture solution of comparative example 3 does not contain graphene quantum dots.
Comparative example 4
The difference from example 1 was that the culture temperature in step (6) of comparative example 4 was constantly 30 ℃ and the light irradiation conditions were the same as in example 1.
The germination of the above-mentioned Moghania mountain sweet potato micro-potatoes of examples 1-3 and comparative examples 1-4 is shown in Table 1.
TABLE 1
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. A method for embedding, accelerating germination and germinating miniature potatoes in Huaiyoshan mountains is characterized by comprising the following steps:
soaking potato microtubers in a first MS culture solution, a second MS culture solution, a third MS culture solution and a fourth MS culture solution respectively; the first MS culture solution contains gibberellin, the second MS culture solution contains chitosan oligosaccharide, the third MS culture solution contains graphene quantum dots, and the fourth MS culture solution contains carbon nano tubes;
mixing the soaked potato micro potatoes with an improved MS culture solution to obtain a micro potato mixture; the improved MS culture solution contains sodium alginate and sucrose but does not contain calcium ions;
dripping the miniature potato mixture into an embedding MS culture solution for embedding to obtain potato miniature potato embedded beads;
planting the potato miniature embedded beads, and alternately culturing under different culture conditions; the culture conditions are respectively illumination culture and dark culture, and the temperature of the illumination culture is higher than that of the dark culture;
and alternately culturing until the potato micro potato embedding beads germinate and emerge.
2. The method for embedding, sprouting and germinating the minipotatoes as recited in claim 1 wherein the soaking temperature of the minipotatoes is 24 to 26 ℃.
3. The method for the embedding, germination accelerating and germination accelerating of the Huaiyoshan sweet potato as claimed in claim 1, wherein the first MS culture solution contains 0.05g/L gibberellin, the second MS culture solution contains 0.05g/L chitosan oligosaccharide, the third MS culture solution contains 0.3-0.5g/L graphene quantum dots, and the fourth MS culture solution contains 0.3-0.5g/L carbon nanotubes.
4. The method for germination acceleration by embedding yushan potato microtubers according to claim 1, wherein the modified MS culture solution contains 0.03g/L sodium alginate and 0.2mol/L sucrose.
5. The method for the embedding germination acceleration of the miniature potatoes in the Yushan mountain of claim 1, wherein the embedding MS culture solution further comprises 0.05g/L gibberellin, 0.05g/L chitosan oligosaccharide, 0.03-0.05g/L graphene quantum dots, 0.03-0.05g/L carbon nanotubes, 0.1mol/LCaCl2And 0.2mol/L sucrose.
6. The method for embedding, sprouting and growing of Huaiyishan high mountain potato according to claim 5 wherein the embedding temperature is 24-26 ℃.
7. The method for the embedding, germination accelerating and germination accelerating of the miniature potatoes in the mountainous Huaiyanshan as claimed in claim 1, wherein the temperature for the light cultivation is 34-36 ℃, the light intensity is 1500-.
8. The method for the embedding, germination accelerating and germination accelerating of the miniature potatoes in the mountains and potatoes in Huaiyanshan as claimed in claim 7, wherein the light culture time is 12h/d, and the dark culture time is 12 h/d.
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Cited By (4)
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| CN111713407A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Method for embedding, transplanting and domesticating stem segments with buds of Huaiyoshan high-mountain potatoes |
| CN111713406A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Embedding vitrification ultra-low temperature detoxification method for Huaiyoshan high-mountain potatoes |
| CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
| CN112753577A (en) * | 2021-01-14 | 2021-05-07 | 上饶师范学院 | Germination method of miniature hemp seed potato |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111713407A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Method for embedding, transplanting and domesticating stem segments with buds of Huaiyoshan high-mountain potatoes |
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| CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
| CN112753577A (en) * | 2021-01-14 | 2021-05-07 | 上饶师范学院 | Germination method of miniature hemp seed potato |
| CN112753577B (en) * | 2021-01-14 | 2022-08-09 | 上饶师范学院 | Germination method of miniature hemp seed potato |
| CN112655562B (en) * | 2021-01-14 | 2023-01-03 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
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Application publication date: 20201027 |