CN109673512A - A method of it improving taro with red buds test tube taro and sprouts effect - Google Patents
A method of it improving taro with red buds test tube taro and sprouts effect Download PDFInfo
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- CN109673512A CN109673512A CN201811654690.7A CN201811654690A CN109673512A CN 109673512 A CN109673512 A CN 109673512A CN 201811654690 A CN201811654690 A CN 201811654690A CN 109673512 A CN109673512 A CN 109673512A
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- taro
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
It improves the method that taro with red buds test tube taro sprouts effect: successively taro with red buds test tube taro being cultivated with seven kinds of fluid nutrient mediums containing graphene quantum dot and carbon nanotube, after 30d, can promote the sprouting of taro with red buds test tube taro.Compared with the prior art, taro with red buds test tube taro sprout time is early, sprouting quantity is more, it is good to sprout effect by the present invention.
Description
Technical field:
The present invention relates to a kind of methods for improving plant test tube taro inducing effect, are exactly a kind of raising taro with red buds examinations
The method of pipe taro sprouting effect.
Background technique:
Taro with red buds is the famous-brand and high-quality agricultural product of Jiangxi Qianshan tradition, is mainly distributed on 13 small towns such as Zixi, Hu Fang, cultivated area
Stablize throughout the year in 5333.3hm2More than.Jiangxi Qianshan taro with red buds medicine food is simultaneous excellent.Edible, (food fiber, polysaccharide are a variety of for nutriment
Amino acid etc.) it is abundant, it is one of southern area of China Main Vegetable Species Suitable For Culture;Medicinal, Jiangxi Qianshan taro with red buds has stomach-benefiting powder
The effects of knot, bowl spares liver and kidney benefiting, sperm adding and marrow healthing.2007, Qianshan County was ordered by the China Green Food committee and Minister Agriculture of China
Entitled whole nation green food raw material taro with red buds standardized production base county;2013, Jiangxi Qianshan taro with red buds was also checked and approved as state
Family's geographical sign agricultural product;2014, Jiangxi Qianshan taro with red buds was awarded " Jiangxi Province's famous agricultural product brand " title and obtains for port vegetable
Dish registration permission.Jiangxi Qianshan taro with red buds generally uses propagation by corm, is easy to happen virosis, makes the yield and quality of taro with red buds
It declines to a great extent.Bring harm and loss are produced to reduce virus to taro with red buds, the use of virus-free seedling will be that Jiangxi Qianshan is red
The development trend of bud taro industry.It needs after taro with red buds detoxic seedling bottle outlet by seedbed nursery, if in film covering, watering, ventilation
Etc. links slightly not note that will affect its survival rate, and test tube seedling be unfavorable for it is long-distance for planting and the indoor preservation of germ plasm resource.
Detoxicating cuvette taro is a kind of material of the preservation germ plasm resource to grow up after detoxification test tube plantlet, has kind of a property height, breeding
Fastly, small in size, it easily stores, produce the advantages that not being subject to seasonal restrictions and can breeding throughout the year, can not only be used to produce virus-free miniature
Taro alleviates the production pressure of spring and summer season test tube seedling, can also utilize tissue culture room existing resource to greatest extent, realizes anniversaryization fortune
Turn, reduces production cost.Key of the test tube taro for ganoid konjaku taro production is its germination rate.Conventional test tube taro sprouts method germination rate
Low, sprout time is inconsistent, and it is poor to sprout effect.For these problems, the present invention develops a kind of raising taro with red buds test tube taro
The method for sprouting effect, can provide technical foundation for the Production of Large Fields of Jiangxi Qianshan taro with red buds detoxification ganoid konjaku taro.
Summary of the invention:
The object of the present invention is to provide a kind of germination rate height, sprout time is more consistent, sprouts the good red bud of raising of effect
The method of taro test tube taro sprouting effect.Realizing the technical solution of the object of the invention is, a kind of raising taro with red buds test tube taro sprouting effect
The method of fruit, it is characterised in that there are following steps: (1) taro with red buds test tube taro is shifted to one: MS+0.3-0.5g/L graphite of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture by alkene quantum dot+30g/L sucrose+0g/L agar;(2) it is cultivating
In base one cultivate 5d after, by taro with red buds test tube taro be transferred to two: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose of culture medium+
Material is put into culturing room after inoculation and carries out normal condition culture by 0g/L agar;It (3), will be red after cultivating 5d in culture medium two
Bud taro test tube taro is transferred to three: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sugarcane of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture by sugar+0g/L agar;It (4), will after cultivating 5d in culture medium three
Taro with red buds test tube taro is transferred to four: 0.4M sucrose+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon of fluid nutrient medium and receives
Mitron+0.1M CaCl2, carry out conventional method embedding;(5) taro with red buds test tube taro embedding pearl is transferred to culture medium five: MS+
Material, is put by 0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar after inoculation
Culturing room carries out normal condition dark culturing;(6) after cultivating 5d in culture medium five, taro with red buds test tube taro embedding pearl is transferred to
Six: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar of culture medium, is put into culturing room for material after inoculation
Carry out normal condition dark culturing;(7) after cultivating 5d on culture medium six, taro with red buds test tube taro embedding pearl is transferred to culture medium
Material is put into culturing room after inoculation and carries out conventional strip by seven: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar
Part dark culturing;(8) on culture medium seven after normal condition dark culturing 5d, taro with red buds test tube taro embedding pearl starts rudiment, sprouts
Bud rate is high, is easy to sow.
Specific embodiment:
In conjunction with following embodiments, the invention will be further described:
(1) it cultivates for the first time
Taro with red buds test tube taro is shifted to one: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L fine jade of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture by rouge are as follows: and 25 ± 2 DEG C of temperature, when illumination
Between 16h/d, light intensity 1500-2500lx.
(2) it cultivates for second
After cultivating 5d in culture medium one, taro with red buds test tube taro is transferred to two: MS+0.3-0.5g/L carbon nanometer of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture by pipe+30g/L sucrose+0g/L agar
Are as follows: 25 ± 2 DEG C of temperature, light application time 16h/d, light intensity 1500-2500lx.
(3) third time is cultivated
After cultivating 5d in culture medium two, taro with red buds test tube taro is transferred to three: MS+0.3-0.5g/L graphene of culture medium
Material is put into culturing room after inoculation and carries out conventional strip by quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar
Part culture, culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature, light application time 16h/d, light intensity 1500-2500lx.
(4) it embeds
After cultivating 5d in culture medium three, taro with red buds test tube taro is transferred to four: 0.4M sucrose+0.3- of fluid nutrient medium
0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+0.1M CaCl2, carry out conventional method embedding.
(5) the 4th cultures
Taro with red buds test tube taro embedding pearl is transferred to five: MS+0.3-0.5g/L graphene quantum dot+0.3- of culture medium
Material is put into culturing room after inoculation and carries out normal condition dark culturing by 0.5g/L carbon nanotube+30g/L sucrose+0g/L agar,
Culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature.
(6) the 5th cultures
After cultivating 5d in culture medium five, taro with red buds test tube taro embedding pearl is transferred to culture medium six: MS+0.3-0.5g/L
Material is put into culturing room after inoculation and carries out normal condition dark culturing, training by graphene quantum dot+30g/L sucrose+0g/L agar
Support room condition of culture are as follows: 25 ± 2 DEG C of temperature.
(7) the 6th cultures
After cultivating 5d on culture medium six, taro with red buds test tube taro embedding pearl is transferred to culture medium seven: MS+0.3-0.5g/L
Material is put into culturing room after inoculation and carries out normal condition dark culturing, culturing room by carbon nanotube+30g/L sucrose+0g/L agar
Condition of culture are as follows: 25 ± 2 DEG C of temperature.
(8) on culture medium seven after normal condition dark culturing 5d, taro with red buds test tube taro embedding pearl starts rudiment, germination rate
Height is easy to sow.
Claims (1)
1. improving the method that taro with red buds test tube taro sprouts effect, it is characterised in that there is following steps: (1) shifting taro with red buds test tube taro
To one: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar of culture medium, material is put into culture after inoculation
Room carries out normal condition culture;(2) after cultivating 5d in culture medium one, taro with red buds test tube taro is transferred to culture medium two: MS+
Material is put into culturing room after inoculation and carries out normal condition culture by 0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar;
(3) after cultivating 5d in culture medium two, taro with red buds test tube taro is transferred to three: MS+0.3-0.5g/L graphene quantum of culture medium
+ 0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar is selected, material is put into culturing room after inoculation and carries out normal condition training
It supports;(4) after cultivating 5d in culture medium three, taro with red buds test tube taro is transferred to four: 0.4M sucrose+0.3- of fluid nutrient medium
0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+0.1M CaCl2, carry out conventional method embedding;(5) by taro with red buds
Test tube taro embedding pearl is transferred to five: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L of culture medium
Material is put into culturing room after inoculation and carries out normal condition dark culturing by sucrose+0g/L agar;(6) it is cultivated in culture medium five
After 5d, by taro with red buds test tube taro embedding pearl be transferred to six: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose of culture medium+
Material is put into culturing room after inoculation and carries out normal condition dark culturing by 0g/L agar;(7) after cultivating 5d on culture medium six,
Taro with red buds test tube taro embedding pearl is transferred to seven: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar of culture medium,
Material is put into culturing room after inoculation and carries out normal condition dark culturing;(8) the normal condition dark culturing 5d on culture medium seven
Afterwards, taro with red buds test tube taro embedding pearl starts rudiment, and germination rate is high, is easy to sow.
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Cited By (2)
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CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
CN112655562B (en) * | 2021-01-14 | 2023-01-03 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
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