CN109673512A - A method of it improving taro with red buds test tube taro and sprouts effect - Google Patents
A method of it improving taro with red buds test tube taro and sprouts effect Download PDFInfo
- Publication number
- CN109673512A CN109673512A CN201811654690.7A CN201811654690A CN109673512A CN 109673512 A CN109673512 A CN 109673512A CN 201811654690 A CN201811654690 A CN 201811654690A CN 109673512 A CN109673512 A CN 109673512A
- Authority
- CN
- China
- Prior art keywords
- taro
- test tube
- culture medium
- red buds
- sucrose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
It improves the method that taro with red buds test tube taro sprouts effect: successively taro with red buds test tube taro being cultivated with seven kinds of fluid nutrient mediums containing graphene quantum dot and carbon nanotube, after 30d, can promote the sprouting of taro with red buds test tube taro.Compared with the prior art, taro with red buds test tube taro sprout time is early, sprouting quantity is more, it is good to sprout effect by the present invention.
Description
Technical field:
The present invention relates to a kind of methods for improving plant test tube taro inducing effect, are exactly a kind of raising taro with red buds examinations
The method of pipe taro sprouting effect.
Background technique:
Taro with red buds is the famous-brand and high-quality agricultural product of Jiangxi Qianshan tradition, is mainly distributed on 13 small towns such as Zixi, Hu Fang, cultivated area
Stablize throughout the year in 5333.3hm2More than.Jiangxi Qianshan taro with red buds medicine food is simultaneous excellent.Edible, (food fiber, polysaccharide are a variety of for nutriment
Amino acid etc.) it is abundant, it is one of southern area of China Main Vegetable Species Suitable For Culture；Medicinal, Jiangxi Qianshan taro with red buds has stomach-benefiting powder
The effects of knot, bowl spares liver and kidney benefiting, sperm adding and marrow healthing.2007, Qianshan County was ordered by the China Green Food committee and Minister Agriculture of China
Entitled whole nation green food raw material taro with red buds standardized production base county；2013, Jiangxi Qianshan taro with red buds was also checked and approved as state
Family's geographical sign agricultural product；2014, Jiangxi Qianshan taro with red buds was awarded " Jiangxi Province's famous agricultural product brand " title and obtains for port vegetable
Dish registration permission.Jiangxi Qianshan taro with red buds generally uses propagation by corm, is easy to happen virosis, makes the yield and quality of taro with red buds
It declines to a great extent.Bring harm and loss are produced to reduce virus to taro with red buds, the use of virus-free seedling will be that Jiangxi Qianshan is red
The development trend of bud taro industry.It needs after taro with red buds detoxic seedling bottle outlet by seedbed nursery, if in film covering, watering, ventilation
Etc. links slightly not note that will affect its survival rate, and test tube seedling be unfavorable for it is long-distance for planting and the indoor preservation of germ plasm resource.
Detoxicating cuvette taro is a kind of material of the preservation germ plasm resource to grow up after detoxification test tube plantlet, has kind of a property height, breeding
Fastly, small in size, it easily stores, produce the advantages that not being subject to seasonal restrictions and can breeding throughout the year, can not only be used to produce virus-free miniature
Taro alleviates the production pressure of spring and summer season test tube seedling, can also utilize tissue culture room existing resource to greatest extent, realizes anniversaryization fortune
Turn, reduces production cost.Key of the test tube taro for ganoid konjaku taro production is its germination rate.Conventional test tube taro sprouts method germination rate
Low, sprout time is inconsistent, and it is poor to sprout effect.For these problems, the present invention develops a kind of raising taro with red buds test tube taro
The method for sprouting effect, can provide technical foundation for the Production of Large Fields of Jiangxi Qianshan taro with red buds detoxification ganoid konjaku taro.
Summary of the invention:
The object of the present invention is to provide a kind of germination rate height, sprout time is more consistent, sprouts the good red bud of raising of effect
The method of taro test tube taro sprouting effect.Realizing the technical solution of the object of the invention is, a kind of raising taro with red buds test tube taro sprouting effect
The method of fruit, it is characterised in that there are following steps: (1) taro with red buds test tube taro is shifted to one: MS+0.3-0.5g/L graphite of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture by alkene quantum dot+30g/L sucrose+0g/L agar；(2) it is cultivating
In base one cultivate 5d after, by taro with red buds test tube taro be transferred to two: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose of culture medium+
Material is put into culturing room after inoculation and carries out normal condition culture by 0g/L agar；It (3), will be red after cultivating 5d in culture medium two
Bud taro test tube taro is transferred to three: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sugarcane of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture by sugar+0g/L agar；It (4), will after cultivating 5d in culture medium three
Taro with red buds test tube taro is transferred to four: 0.4M sucrose+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon of fluid nutrient medium and receives
Mitron+0.1M CaCl2, carry out conventional method embedding；(5) taro with red buds test tube taro embedding pearl is transferred to culture medium five: MS+
Material, is put by 0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar after inoculation
Culturing room carries out normal condition dark culturing；(6) after cultivating 5d in culture medium five, taro with red buds test tube taro embedding pearl is transferred to
Six: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar of culture medium, is put into culturing room for material after inoculation
Carry out normal condition dark culturing；(7) after cultivating 5d on culture medium six, taro with red buds test tube taro embedding pearl is transferred to culture medium
Material is put into culturing room after inoculation and carries out conventional strip by seven: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar
Part dark culturing；(8) on culture medium seven after normal condition dark culturing 5d, taro with red buds test tube taro embedding pearl starts rudiment, sprouts
Bud rate is high, is easy to sow.
Specific embodiment:
In conjunction with following embodiments, the invention will be further described:
(1) it cultivates for the first time
Taro with red buds test tube taro is shifted to one: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L fine jade of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture by rouge are as follows: and 25 ± 2 DEG C of temperature, when illumination
Between 16h/d, light intensity 1500-2500lx.
(2) it cultivates for second
After cultivating 5d in culture medium one, taro with red buds test tube taro is transferred to two: MS+0.3-0.5g/L carbon nanometer of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture by pipe+30g/L sucrose+0g/L agar
Are as follows: 25 ± 2 DEG C of temperature, light application time 16h/d, light intensity 1500-2500lx.
(3) third time is cultivated
After cultivating 5d in culture medium two, taro with red buds test tube taro is transferred to three: MS+0.3-0.5g/L graphene of culture medium
Material is put into culturing room after inoculation and carries out conventional strip by quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar
Part culture, culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature, light application time 16h/d, light intensity 1500-2500lx.
(4) it embeds
After cultivating 5d in culture medium three, taro with red buds test tube taro is transferred to four: 0.4M sucrose+0.3- of fluid nutrient medium
0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+0.1M CaCl2, carry out conventional method embedding.
(5) the 4th cultures
Taro with red buds test tube taro embedding pearl is transferred to five: MS+0.3-0.5g/L graphene quantum dot+0.3- of culture medium
Material is put into culturing room after inoculation and carries out normal condition dark culturing by 0.5g/L carbon nanotube+30g/L sucrose+0g/L agar,
Culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature.
(6) the 5th cultures
After cultivating 5d in culture medium five, taro with red buds test tube taro embedding pearl is transferred to culture medium six: MS+0.3-0.5g/L
Material is put into culturing room after inoculation and carries out normal condition dark culturing, training by graphene quantum dot+30g/L sucrose+0g/L agar
Support room condition of culture are as follows: 25 ± 2 DEG C of temperature.
(7) the 6th cultures
After cultivating 5d on culture medium six, taro with red buds test tube taro embedding pearl is transferred to culture medium seven: MS+0.3-0.5g/L
Material is put into culturing room after inoculation and carries out normal condition dark culturing, culturing room by carbon nanotube+30g/L sucrose+0g/L agar
Condition of culture are as follows: 25 ± 2 DEG C of temperature.
(8) on culture medium seven after normal condition dark culturing 5d, taro with red buds test tube taro embedding pearl starts rudiment, germination rate
Height is easy to sow.
Claims (1)
1. improving the method that taro with red buds test tube taro sprouts effect, it is characterised in that there is following steps: (1) shifting taro with red buds test tube taro
To one: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar of culture medium, material is put into culture after inoculation
Room carries out normal condition culture；(2) after cultivating 5d in culture medium one, taro with red buds test tube taro is transferred to culture medium two: MS+
Material is put into culturing room after inoculation and carries out normal condition culture by 0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar；
(3) after cultivating 5d in culture medium two, taro with red buds test tube taro is transferred to three: MS+0.3-0.5g/L graphene quantum of culture medium
+ 0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar is selected, material is put into culturing room after inoculation and carries out normal condition training
It supports；(4) after cultivating 5d in culture medium three, taro with red buds test tube taro is transferred to four: 0.4M sucrose+0.3- of fluid nutrient medium
0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+0.1M CaCl2, carry out conventional method embedding；(5) by taro with red buds
Test tube taro embedding pearl is transferred to five: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L of culture medium
Material is put into culturing room after inoculation and carries out normal condition dark culturing by sucrose+0g/L agar；(6) it is cultivated in culture medium five
After 5d, by taro with red buds test tube taro embedding pearl be transferred to six: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose of culture medium+
Material is put into culturing room after inoculation and carries out normal condition dark culturing by 0g/L agar；(7) after cultivating 5d on culture medium six,
Taro with red buds test tube taro embedding pearl is transferred to seven: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar of culture medium,
Material is put into culturing room after inoculation and carries out normal condition dark culturing；(8) the normal condition dark culturing 5d on culture medium seven
Afterwards, taro with red buds test tube taro embedding pearl starts rudiment, and germination rate is high, is easy to sow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811654690.7A CN109673512A (en) | 2018-12-17 | 2018-12-17 | A method of it improving taro with red buds test tube taro and sprouts effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811654690.7A CN109673512A (en) | 2018-12-17 | 2018-12-17 | A method of it improving taro with red buds test tube taro and sprouts effect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109673512A true CN109673512A (en) | 2019-04-26 |
Family
ID=66190436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811654690.7A Pending CN109673512A (en) | 2018-12-17 | 2018-12-17 | A method of it improving taro with red buds test tube taro and sprouts effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109673512A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
| CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314845A (en) * | 2013-02-21 | 2013-09-25 | 江苏省中国科学院植物研究所 | Method for shortening tissue-culture propagating period of colorful calla lily by using single walled carbon nanotube |
| CN104145820A (en) * | 2014-08-18 | 2014-11-19 | 安徽省农业科学院园艺研究所 | Tissue culture and rapid propagation method of taro with red buds |
| CN104145680A (en) * | 2014-08-18 | 2014-11-19 | 江西省江天农业科技有限公司 | Method for propagating detoxification colocasia konishii hayata seeds |
| CN108575759A (en) * | 2018-06-04 | 2018-09-28 | 江苏省中国科学院植物研究所 | A method of carrying out bletilla striata tissue-culturing rapid propagation using carbon nanotube |
| CN108887180A (en) * | 2018-06-27 | 2018-11-27 | 上饶师范学院 | A method of improving radix tetrastigme explant sterilization effect |
| CN108901843A (en) * | 2018-06-27 | 2018-11-30 | 上饶师范学院 | A method of improving radix tetrastigme root tuber in-vitro inducing effect |
-
2018
- 2018-12-17 CN CN201811654690.7A patent/CN109673512A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314845A (en) * | 2013-02-21 | 2013-09-25 | 江苏省中国科学院植物研究所 | Method for shortening tissue-culture propagating period of colorful calla lily by using single walled carbon nanotube |
| CN104145820A (en) * | 2014-08-18 | 2014-11-19 | 安徽省农业科学院园艺研究所 | Tissue culture and rapid propagation method of taro with red buds |
| CN104145680A (en) * | 2014-08-18 | 2014-11-19 | 江西省江天农业科技有限公司 | Method for propagating detoxification colocasia konishii hayata seeds |
| CN108575759A (en) * | 2018-06-04 | 2018-09-28 | 江苏省中国科学院植物研究所 | A method of carrying out bletilla striata tissue-culturing rapid propagation using carbon nanotube |
| CN108887180A (en) * | 2018-06-27 | 2018-11-27 | 上饶师范学院 | A method of improving radix tetrastigme explant sterilization effect |
| CN108901843A (en) * | 2018-06-27 | 2018-11-30 | 上饶师范学院 | A method of improving radix tetrastigme root tuber in-vitro inducing effect |
Non-Patent Citations (1)
| Title |
|---|
| 乔俊等: "纳米炭材料对作物生长影响的研究进展", 《农业工程学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
| CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
| CN112655562B (en) * | 2021-01-14 | 2023-01-03 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101564008B (en) | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings | |
| CN104335903B (en) | It is a kind of to promote Pseudobulbus Bletillae (Rhizoma Bletillae) rapid propagation method | |
| CN101258835B (en) | Fast reproducing method for high quality seedling of dendrobium officinale | |
| CN103416305B (en) | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings | |
| CN103583365B (en) | Production method of bletilla seed stems | |
| CN104429965B (en) | The method that high eyebrow Herba Anoectochili roxburghii seed is bred without hormone quickly tissue culture | |
| CN104496625B (en) | A kind of nutrient solution prescription of blueberry Water culture | |
| CN103704139B (en) | The blue test-tube plantlet breeding method of multi-pattern type pocket | |
| CN105145361B (en) | A kind of method of reproducing bletilla striata tissue-cultured seedling and the implantation methods of the bletilla striata | |
| CN113080061B (en) | Pandanus communis cultivation method | |
| CN102550409A (en) | Method of tissue culture propagation for rhizome polygonati | |
| CN102893865A (en) | Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl. | |
| CN107896824A (en) | A kind of cultural method of Termitomyces albuminosus with black skin | |
| CN105993964A (en) | Technology for carrying out tissue culture breeding by adopting common bletilla pseudobulb seeds | |
| CN109673512A (en) | A method of it improving taro with red buds test tube taro and sprouts effect | |
| CN106305427B (en) | A kind of method of Amur maple tissue-culturing quick-propagation | |
| CN108029559A (en) | A kind of method of quickly breeding bearberry tissue-cultured seedling | |
| CN105706872B (en) | Natural propagation method for culturing seedlings is broadcast live in bletilla striata seeds | |
| CN108575759B (en) | A method of bletilla striata tissue-culturing rapid propagation is carried out using carbon nanotube | |
| CN106665367B (en) | A kind of Golden Bell Tree quick breeding method for tissue culture | |
| CN103416310B (en) | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture | |
| CN105052515B (en) | A kind of breeding method of the healthy seedling of color green pepper | |
| CN103548693A (en) | Tissue culture rapid-multiplying method for bletilla striata protocorm-like bodies | |
| CN106665364B (en) | The method that Lilium brownii var viridulum filial generation bulb expands development and takes root | |
| CN105296360A (en) | Phellinus linteus mother strain tissue isolation transfer-tube culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |