CN106665364B - The method that Lilium brownii var viridulum filial generation bulb expands development and takes root - Google Patents
The method that Lilium brownii var viridulum filial generation bulb expands development and takes root Download PDFInfo
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- CN106665364B CN106665364B CN201710090491.7A CN201710090491A CN106665364B CN 106665364 B CN106665364 B CN 106665364B CN 201710090491 A CN201710090491 A CN 201710090491A CN 106665364 B CN106665364 B CN 106665364B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention provides a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root.Including following method and step：1）Lilium brownii var viridulum filial generation rescue culture, seedling processed；2）Clump bud is proliferated, fast breeding seedling numbers, clump bud seedling processed；3）Bulblet induction cultivates differentiation bulb；4）Clove expands, takes root；It cultivates larger bulb and takes root, the breeding original material as production directly moves into field planting；Hybridize bulblet of lilium by optimization and quickly expand the simultaneously influence factors such as culture of rootage, to provide technical support for Lilium brownii var viridulum filial generation test tube seedling large-scale production.And it provides technology for edible Lilium brownii var viridulum filial generation test tube seedling large-scale production and ensures；Meet needs of the market to edible lily hybrid new breed；To reduce Lilium brownii var viridulum planting cost.
Description
Technical field：
The present invention relates to the lily culture method of agricultural plant cultivation field, after especially a kind of hybridization using Lilium brownii var viridulum
The method expanded development for bulb and taken root.
Background technology：
Lily is Liliaceae lilium（Scientific name：Lilium）Perennial herb plant bulbs.Lilium brownii var viridulum is that China three is big
One of main cultivation edible lily kind, has long enjoyed a good reputation, be eat, be medicinal, the famous-particular-excellent kind in bird watching and nutritive value
One of best kind.Its bulb is spherical in shape, 4~6 centimetres of general transverse diameter, scale lanceolar, white, when exposure in yellow-white or
Slightly band pink.Content of starch is high, and full of nutrition, lightly seasoned not bitter, the aerial stem of plant is about 90~120 centimetres high, and leaf is scattered, drapes over one's shoulders needle
Shape.Flower milky is aromatic, bulb edible and medicinal.According to medical ancient books and records《Synopsis Golden Chamber》,《Compendium of Materia Medica》,《Middle traditional Chinese medicines
Allusion quotation》It records, lily moistens the lung and relieve the cough, antitoxic heart-soothing and sedative, the stomach invigorating of reason spleen, tonifying middle-Jiao and Qi, sharp stool and urine, solves nameless sores or boils and hemostasis etc.
Effect.Modern medicine study proves that colchicin, demecolcine that lily contains etc. have significantly human body cell mitosis
Inhibiting effect inhibits cancer cell and acute gout etc., there is obvious curative effects.Lily can also refine the medicines such as glucose and quinine
Product.Its bulb head is big, length of a film, meat are thick, the heart is real, color and luster is white, taste good, is full of nutrition, drug effect is apparent, after testing, per hectogram
Water content 65% in lily, carbon aquation inclusion 23.8%, total dietary fiber 5.9%, wherein pectin 4.8%, protein 4%, fat
0.1%, moisture 1.1%, calcium 0.9%, iron 0.09%, 132 kilocalories of heat.Therefore, lily is the green guarantor for integrating food, drug
Health food.
Lilium brownii var viridulum is known as " south ginseng ", " treasure in food ".Currently, related Lilium brownii var viridulum clove is quickly swollen
The research of big and culture of rootage optimization etc. is less.The method that the country builds clone groups of lily hybridization generation in recent years simultaneously
Report more, but most research for laying particular emphasis on flower lily, rarely seen edible lily correlative study.The Lanzhou related Lilium brownii var viridulum x hundred
Conjunction filial generation tissue culture bulb quickly expands development and the method taken root is even more to have no relevant report.
On the other hand, the cultivation period of edible lily filial generation bulb is longer, and one kind of selection and breeding at least needs 6-7
Time, so long breeding cycle seriously restrict the scale of edible lily breeding industry, industrialized development, it is difficult to meet city
Demand of the field to self-fertile lily new varieties.
Therefore, how to accelerate the breeding process of lily cultivar, and existing Lilies breeding technology is improved, with contracting
The cultivation time of short lily new varieties.Perfect Lilium brownii var viridulum filial generation fast breeding technique method is set up, is hybridized by optimizing
Bulblet of lilium quickly expands and the influence factors such as culture of rootage are studied in detail, to adapt to Lilium brownii var viridulum filial generation test tube
Seedling large-scale production, to meet the needs of market is to cultivating lily new varieties.
Invention content：
The present invention provides a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root.By optimizing hybridization hundred
It closes clove and quickly expands the simultaneously influence factors such as culture of rootage, to be carried for Lilium brownii var viridulum filial generation test tube seedling large-scale production
For technical support.And it provides technology for edible Lilium brownii var viridulum filial generation test tube seedling large-scale production and ensures；Meet market to food
With the needs of hybrid lily new varieties；To reduce Lilium brownii var viridulum planting cost.
The present invention discloses a kind of method that Lilium brownii var viridulum filial generation bulb expands development, takes root, and is hybridized with Lilium brownii var viridulum
Offspring's capsule is explant raw material, Lilium brownii var viridulum filial generation processed group, including following method and step：1）After Lilium brownii var viridulum hybridization
For rescue culture, seedling processed；2）Clump bud is proliferated, fast breeding seedling numbers, clump bud seedling processed；3）Bulblet induction cultivates differentiation squama
Stem；4）Clove expands, takes root；It cultivates larger bulb and takes root, the breeding original material as production directly moves into greatly
It plants in field；Described 1）Lilium brownii var viridulum filial generation rescue culture is that seed is taken out from capsule, squeezes out rataria, is inoculated into culture medium
On, control condition of culture is that cultivation temperature is 20-25 DEG C, is first transferred to illumination cultivation to after sprouting in dark condition culture, controls
Condition is intensity of illumination 2000-5000Lx, the condition of 12 hour/day of light application time, cultivates 30-80 days, obtains seedling.
The method that a kind of Lilium brownii var viridulum filial generation bulb of the present invention expands development and takes root, described 2）Clump bud is proliferated
By 1）Seedling inoculation is cultivated in corresponding culture medium, and condition of culture is：Using optical culture, control cultivation cycle is 25-40
It, cultivation temperature is 22-28 DEG C, intensity of illumination 2000-4000Lux, and the photoperiod is that 12 light/12h is dark, and clump bud seedling is made.
The method that a kind of Lilium brownii var viridulum filial generation bulb of the present invention expands development and takes root, preferably described 3）Clove
Induction, is by 2）The clump bud seedling of clump bud proliferation system cuts blade, and remaining sprout switching is cultivated in corresponding culture medium, carried out
Bulblet induction is formed and proliferation, system differentiation bulb；Using optical culture, control optical culture condition is：Cultivation cycle is 30-40
It, cultivation temperature is 20-30 DEG C, and culture intensity of illumination is 2000-4000Lux, and the photoperiod is that 12 light/12h is dark.
The method that a kind of Lilium brownii var viridulum filial generation bulb of the present invention expands development and takes root, preferably described 4）Clove is swollen
Greatly, it takes root：It is by 3）The differentiation bulb of gained is cut, and after cutting off blade, and transplanting, which is expanded in bulb in Development culture base, cultivates,
It carries out expanding for clove to grow and take root, the constituent component that control bulb expands Development culture base is to train comprising 1-2 times of MS
Support base, 1.0-5.0g/L paclobutrazols, 0.2-0.8mg/L methyl α-naphthyl acetates, 60-120g/L sucrose, activated carbon 1.0-5.0g/L and 3.5-
10.5g/L agar, pH=5.6-6.2.
Further, it is the Lilium brownii var viridulum filial generation processed group, is that use Lilium brownii var viridulum filial generation capsule be outer
Implant raw material carries out rescue culture, and hybrid embryo processed is numerous through adventitious bud expansion, then it is seed ball to carry out balling culture, waits for that seed ball weight is 2-
When 3g, vernalization treatment is carried out, that is, Lilium brownii var viridulum filial generation group is made.
Preferably, be the Lilium brownii var viridulum filial generation be Lilium brownii var viridulum and lanzhou lily filial generation.
Preferably, it is control 1）Seedling inoculation carries out culture 1-2 times in corresponding culture medium.
Further, it is that control bulb is expanded and cultivated more than secondary in Development culture base.
Preferably, it is 40-60 days the control vernalization treatment time to be.
The present invention uses the above method, through scientific experiment is repeated, i.e., expands Development culture using the bulb of the present invention
Base after cultivating 60 days, can make Lilium brownii var viridulum x lanzhou lilies filial generation tissue culture bulb increase to 300 mg/ by 55mg/
It is a, net gain 245mg/；Increase to 432mg/ net gain 330mg/ by 102mg/, and it is good to take root.
The present invention uses corresponding culture test tube seedling, culture by improving Lilium brownii var viridulum filial generation tissue culture and rapid propagation method
The bulb for having and expanding is obtained after the completion, and the breeding original material that can be used as production directly moves into field planting, significantly carries
High breeding coefficient, shortens the growth cycle of Lilium brownii var viridulum filial generation.Planting benefit is improved, Lilium brownii var viridulum industry can be met
Change, large-scale planting and with plant a demand.Be conducive to the extensive plantation and popularization of Lilium brownii var viridulum, with good economic efficiency and society
It can benefit.
Specific implementation mode：
With reference to specific example, the invention will be further described.
Each material of the present invention can be prepared by commercially available acquisition, or by art methods.
A kind of Lilium brownii var viridulum filial generation bulb of the present invention expands the method that development is taken root, with Lilium brownii var viridulum filial generation capsule
Fruit is explant raw material, Lilium brownii var viridulum filial generation processed group, including following method and step：1）Lilium brownii var viridulum filial generation embryo is drawn
It rescues, seedling processed；2）Clump bud is proliferated, clump bud seedling processed；3）Bulblet induction, system differentiation bulb；4）Clove expands growth and life
Root.Described 1）Lilium brownii var viridulum filial generation rescue culture is that seed is taken out from capsule, squeezes out rataria, is inoculated on culture medium,
Control condition of culture is that cultivation temperature is 20-25 DEG C, and first dark culturing is transferred to illumination cultivation to after sprouting, and control condition is light
It is 2000-5000Lx according to intensity, under conditions of 8-12 hour/day of light application time, culture 30-80 days obtains seedling.
Lilium brownii var viridulum filial generation group described in the method for the present invention is to use Lilium brownii var viridulum filial generation capsule for explant
Material carries out rescue culture to acquisition hybridization fruit, and the hybrid embryo adventitious bud expansion that rescue culture obtains is numerous, then carries out balling culture, when
When seed ball weight grows into 2-3g or so, then through temperature be 3-5 DEG C at, vernalization treatment 40-60 days, that is, formed Lilium brownii var viridulum hybridize
Progeny population.
Lilium brownii var viridulum filial generation bulb of the present invention expands the method or cry Lilium brownii var viridulum filial generation that development is taken root
The method that tissue culture bulb expands development, including such as the next stage：
1）Lilium brownii var viridulum filial generation rescue culture, seedling processed：Seed is taken out from the capsule of Lilium brownii var viridulum filial generation, is squeezed
Go out rataria, be inoculated on suitable corresponding culture medium, be 20-25 DEG C in cultivation temperature, first dark culturing turns after its sprouting
Enter illumination cultivation, control intensity of illumination is 2000-5000Lx, under conditions of 8~12 hour/day of light application time, cultivates 30-80
It, obtains seedling；
2）Clump bud is proliferated, clump bud seedling processed, fast breeding seedling numbers；By above-mentioned 1）Seedling is obtained, by seedling inoculation to suitable
It is cultivated in suitable corresponding culture medium, condition of culture is：Cultivation cycle is 30 days, and cultivation temperature is 25 DEG C, intensity of illumination
For 2000-4000Lux, the photoperiod is that 12h light/12h is dark, and stage culture control carries out 2 times, can also be 2 times or more, is formed
Clump bud seedling；
3）Bulblet induction, system differentiation bulb：By 2）The clump bud seedling cutting of gained removes blade, and remaining sprout is transferred
To suitable corresponding culture medium, the induced synthesis and proliferation of bulb are carried out, condition of culture is：Cultivation cycle is 30-40 days, training
It is 25 DEG C to support temperature, and culture intensity of illumination is 2000-4000Lux, and the photoperiod is that 12h light/12h is dark；Bulb must be broken up；
4）Clove, which is expanded, to take root；Expanding for clove grows and takes root：By 3）The differentiation bulb of gained is cut down, and
After cutting off blade, transplanting, which is expanded in bulb in Development culture base, cultivates, and carries out expanding for clove and grows and take root, control culture
Condition is：Cultivation cycle is 60 days, and cultivation temperature is 25 DEG C, is protected from light light culture, and the bulb expands Development culture base its component
Composition includes 1 times of MS culture medium, 1g/L paclobutrazols, 0.2mg/L methyl α-naphthyl acetates, 80g/L sucrose, activated carbon 2g/L and 6.5g/L fine jade
Fat, pH=5.8；Stage culture carries out for 2 times or more.
The present invention is by improving Lilium brownii var viridulum filial generation group culturation rapid propagating technology scheme, operating process and culture test tube seedling class
Seemingly, the bulb for having and expanding is obtained after the completion of culture, the breeding original material that can be used as production directly moves into field planting, greatly
Amplitude improves breeding coefficient, shortens the growth cycle of Lilium brownii var viridulum filial generation, is conducive to the wide of Lilium brownii var viridulum cenospecies
General plantation and popularization, with good economic efficiency and social benefit.
The culture medium used in the embodiment above does not specify place such as the above-mentioned corresponding training used in addition to published
Foster base is using the prior art commercially available culture medium applied to for the use of the technology of the present invention in the market.
Claims (7)
1. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root, is outer with Lilium brownii var viridulum filial generation capsule
Implant raw material, Lilium brownii var viridulum filial generation processed group, including following method and step：1）Lilium brownii var viridulum filial generation rescue culture, system
Seedling；2）Clump bud is proliferated, fast breeding seedling numbers, clump bud seedling processed； 3）Bulblet induction cultivates differentiation bulb；4）Clove
It expands, take root；Described 1）Lilium brownii var viridulum filial generation rescue culture is that seed is taken out from capsule, squeezes out rataria, is inoculated into culture
On base, control condition of culture is that cultivation temperature is 20-25 DEG C, is first transferred to illumination cultivation to after sprouting in dark condition culture, controls
Condition processed is intensity of illumination 2000-5000Lx, under conditions of 8-12 hours/day of light application time, cultivates 30-80 days, obtains children
Seedling；
Described 3）Bulblet induction is by 2）The clump bud seedling of clump bud proliferation system cuts blade, and remaining sprout switching is in corresponding
It is cultivated in culture medium, carries out Bulblet induction formation and proliferation, system differentiation bulb；Using optical culture, control optical culture condition is：
Cultivation cycle be 30-40 days, cultivation temperature be 20-30 DEG C, culture intensity of illumination be 2000-4000Lux, the photoperiod for 12h light/
12h is dark；
Described 4）Clove expands, takes root：It is by 3）The differentiation bulb of gained is cut, and after cutting off blade, and transplanting is swollen in bulb
It is cultivated in partial gigantism culture medium, carries out expanding for clove and grow and take root, control bulb expands the group grouping of Development culture base
As 1-2 times of MS culture mediums, the paclobutrazol of 1.0-5.0g/L, the methyl α-naphthyl acetate of 0.2-0.8mg/L, the sucrose of 60-120g/L, work
The agar of property charcoal 1.0-5.0g/L and 3.5-10.5g/L, pH=5.6-6.2.
2. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root according to claim 1, it is characterized in that
Described 2）Clump bud proliferation is by 1）Seedling inoculation is cultivated in corresponding culture medium, and condition of culture is：Using optical culture, control
Cultivation cycle processed is 25-40 days, and cultivation temperature is 22-28 DEG C, intensity of illumination 2000-4000Lux, and the photoperiod is 12 light/12h
Secretly, clump bud seedling is made in fast breeding seedling numbers.
3. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root according to claim 1, it is characterized in that
The Lilium brownii var viridulum filial generation processed group is to use Lilium brownii var viridulum filial generation capsule for explant raw material, carries out rescue culture,
Hybrid embryo processed, it is numerous through adventitious bud expansion, then it is that seed ball carries out vernalization treatment when control seed ball girth diameter is 2-3g to carry out balling culture,
Lilium brownii var viridulum filial generation group is made.
4. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root according to claim 1, it is characterized in that
The Lilium brownii var viridulum filial generation is Lilium brownii var viridulum and lanzhou lily filial generation.
5. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root according to claim 2, it is characterized in that
Control 1）Seedling inoculation carries out culture 1-2 times in corresponding culture medium.
6. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root according to claim 1, it is characterized in that
Control bulb, which expands, to be cultivated in Development culture base more than secondary.
7. a kind of method that Lilium brownii var viridulum filial generation bulb expands development and takes root according to claim 3, it is characterized in that
It is 40-60 days to control the vernalization treatment time.
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| CN113841613B (en) * | 2021-10-26 | 2022-07-15 | 甘肃省农业科学院生物技术研究所 | Efficient breeding method for lilium davidii germchit |
| CN115669539A (en) * | 2022-08-25 | 2023-02-03 | 兰州市农业科技研究推广中心 | Test tube bulb expansion culture method for hybrid progeny of Lanzhou lily |
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| CN102124956B (en) * | 2011-02-15 | 2013-01-09 | 北京林业大学 | In vitro culture method for hybrid embryos of lilies |
| CN102742440B (en) * | 2012-07-02 | 2014-01-01 | 西北农林科技大学 | Breeding and culture method for oriental lily bulblet and bulb |
| CN103636502B (en) * | 2013-12-11 | 2015-10-28 | 广西壮族自治区农业科学院生物技术研究所 | A kind of promote lanzhou lily group train bulb expand grow method |
| CN104920228B (en) * | 2015-07-17 | 2017-03-29 | 中国科学院昆明植物研究所 | A kind of Baoxing Bulbus Lilii tissue-culturing rapid propagation and in-vitro conservation method |
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