CN109673511A - A method of improving test tube potato inducing effect - Google Patents
A method of improving test tube potato inducing effect Download PDFInfo
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- CN109673511A CN109673511A CN201811654689.4A CN201811654689A CN109673511A CN 109673511 A CN109673511 A CN 109673511A CN 201811654689 A CN201811654689 A CN 201811654689A CN 109673511 A CN109673511 A CN 109673511A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The method for improving test tube potato inducing effect: successively potato stem with bud embedding pearl is cultivated with six kinds of culture mediums containing graphene quantum dot and carbon nanotube, after 120d, more biggish Potato microtuber can be formed.Compared with the prior art, potato test tube seedling formation Potato microtuber quantity is more, diameter is big, effect is good by the present invention.
Description
Technical field:
The present invention relates to a kind of methods for improving plant Stem covered by vermiculite effect, are exactly a kind of raising potato examinations
The method of pipe potato inducing effect.
Background technique:
Huaiyu Shan Mountain high mountain potato is called numb seed potato, main planting area be the domestic village Yu Feng, the township Yushan County Huai Yu,
The village Yang Tang, the village Jin Ping, the village Long Shou, 5, critical point village administrative village.Huaiyu Shan Mountain high mountain potato tubers protein rich in relies
Propylhomoserin and tryptophan, potassium, zinc, iron, VC、VB1、VB2Deng, and starch and fat content are low, are free of reduced sugar, are suitble to " three high " crowd
It is edible.In addition, cellulose contained by numb seed potato stem tuber is delicate, has no stimulation to gastrointestinal mucosa, have and alleviate pain or reduce gastric acid
Secreting function.Therefore, Huaiyu Shan Mountain high mountain potato has and middle nourishing the stomach, invigorating spleen to remove dampness, hypoglycemic, beautifying face and moistering lotion, prevention and treatment stomach
Cancer and other effects.In April, 2013, Huaiyu Shan Mountain high mountain potato are approved as national geography mark agricultural product.Huaiyu Shan Mountain high mountain potato
Breeding mainly by way of vegetative propagation, long-term vegetative propagation be easy to cause virus to accumulate in plant by generation, from
And kind of a sexual involution occurs, the substantially underproduction and quality decline are caused, and tuber blight also easily occurs to rot during storing, to life
It produces and sale all brings about great losses.Therefore, how detoxification is carried out to Huaiyu Shan Mountain high mountain potato, becomes Huaiyu Shan Mountain high mountain Ma Ling
The most important thing of potato realization industrialization.Detoxicating cuvette potato is a kind of preservation germ plasm resource to grow up after detoxification test tube plantlet
Material, have many advantages, such as kind that property is high, breeding is fast, small in size, easily storage, production is not subject to seasonal restrictions and can breed throughout the year,
It can not only be used to produce toxicity micro potato, alleviate the production pressure of spring and summer season test tube seedling, tissue culture can also be utilized to greatest extent
Room existing resource realizes anniversaryization operating, reduces production cost.Two keys that Stem covered by vermiculite is formed are Stem covered by vermiculite
Quantity and size.The conventional easy quantity of Stem covered by vermiculite formation method is few, and yampi rate is low.For these problems, present invention exploitation
A kind of method improving test tube potato inducing effect, can provide skill for the production of Huaiyu Shan Mountain high mountain virus-free seed potato
Art basis.
Summary of the invention:
The raising test tube potato inducing effect that the object of the present invention is to provide a kind of quantity is more, diameter is big, effect is good
Method.Realizing the technical solution of the object of the invention is, a method of test tube potato inducing effect being improved, feature exists
In there is following steps: (1) cut the stem with bud of potato test tube seedling conventional length, be transferred to one: 0.4M sucrose of culture medium+
0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+0.1M CaCl2, carry out conventional method embedding；(2) by horse
Bell potato stem with bud embedding pearl is transferred to two: MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L fine jade of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture by rouge；(3) after cultivating 15d on culture medium two, by potato band
Leaf stem section embedding pearl is transferred to three: MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar of culture medium, after inoculation
Material is put into culturing room and carries out normal condition culture；(4) after cultivating 15d on culture medium three, by potato stem with bud packet
Bury pearl be transferred to four: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose of culture medium+
Material is put into culturing room after inoculation and carries out normal condition culture by 7.5g/L agar；It (5), will after cultivating 15d on culture medium four
Potato stem with bud embedding pearl is transferred to five: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanometer of culture medium
Material is put into culturing room after inoculation and carries out normal condition dark culturing by pipe+90g/L sucrose+7.5g/L agar；(6) it is cultivating
After cultivating 15d on base five, potato stem with bud embedding pearl is transferred to six: MS+0.3-0.5g/L graphene quantum of culture medium
+ 90g/L sucrose+7.5g/L agar is selected, material is put into culturing room after inoculation and carries out normal condition dark culturing；(7) it is cultivating
On base six cultivate 15d after, by potato stem with bud embedding pearl be transferred to seven: MS+0.3-0.5g/L carbon nanotube of culture medium+
Material is put into culturing room after inoculation and carries out normal condition dark culturing by 90g/L sucrose+7.5g/L agar；(8) potato band bud
Stem section embeds pearl on culture medium seven after normal condition dark culturing 60d, it is white potato shaped at Potato microtuber quantity it is more, diameter is big,
Effect is good.
Specific embodiment:
In conjunction with following embodiments, the invention will be further described:
(1) it embeds
The stem with bud for cutting potato test tube seedling conventional length is transferred to one: 0.4M sucrose+0.3-0.5g/L of culture medium
Graphene quantum dot+0.3-0.5g/L carbon nanotube+0.1M CaCl2, carry out conventional method embedding.
(2) it cultivates for the first time
Potato stem with bud embedding pearl is transferred to two: MS+0.3-0.5g/L graphene quantum dot+30g/L of culture medium
Material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture are as follows: temperature 25 by sucrose+30g/L agar
± 2 DEG C, light application time 16h/d, light intensity 1500-2500lx.
(3) it cultivates for second
After cultivating 15d on culture medium two, potato stem with bud embedding pearl is transferred to culture medium three: MS+0.3-
Material is put into culturing room after inoculation and carries out normal condition culture, training by 0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar
Support room condition of culture are as follows: 25 ± 2 DEG C of temperature, light application time 16h/d, light intensity 1500-2500lx.
(4) third time is cultivated
After cultivating 15d on culture medium three, potato stem with bud embedding pearl is transferred to culture medium four: MS+0.3-
0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar, is put into training for material after inoculation
It supports room and carries out normal condition culture, culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature, light application time 16h/d, light intensity 1500-
2500lx。
(5) the 4th cultures
After cultivating 15d on culture medium four, potato stem with bud embedding pearl is transferred to culture medium five: MS+0.3-
0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+90g/L sucrose+7.5g/L agar, is put into training for material after inoculation
It supports room and carries out normal condition dark culturing, culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature.
(6) the 5th cultures
After cultivating 15d on culture medium five, potato stem with bud embedding pearl is transferred to culture medium six: MS+0.3-
0.5g/L graphene quantum dot+90g/L sucrose+7.5g/L agar, after inoculation by material be put into culturing room carry out normal condition it is black
Dark culture, culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature.
(7) the 6th cultures
After cultivating 15d on culture medium six, potato stem with bud embedding pearl is transferred to culture medium seven: MS+0.3-
Material is put into culturing room after inoculation and carries out the training of normal condition dark by 0.5g/L carbon nanotube+90g/L sucrose+7.5g/L agar
It supports, culturing room's condition of culture are as follows: 25 ± 2 DEG C of temperature；
(8) potato stem with bud embeds pearl on culture medium seven after normal condition dark culturing 60d, it is white potato shaped at
Potato microtuber quantity is more, diameter is big, effect is good.
Claims (1)
1. the method for improving test tube potato inducing effect, it is characterised in that have following steps: (1) cutting potato test tube seedling
The stem with bud of conventional length is transferred to one: 0.4M sucrose+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L of culture medium
Carbon nanotube+0.1M CaCl2, carry out conventional method embedding；(2) potato stem with bud embedding pearl is transferred to culture medium two:
Material is put into culturing room after inoculation and carries out routine by MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L agar
CMC model；(3) after cultivating 15d on culture medium two, potato stem with bud embedding pearl is transferred to culture medium three: MS+
Material is put into culturing room after inoculation and carries out normal condition training by 0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar
It supports；(4) after cultivating 15d on culture medium three, potato stem with bud embedding pearl is transferred to culture medium four: MS+0.3-0.5g/
L graphene quantum dot+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar, is put into culturing room for material after inoculation
Carry out normal condition culture；(5) after cultivating 15d on culture medium four, potato stem with bud embedding pearl is transferred to culture medium
Five: MS+0.3-0.5g/L graphene quantum dot+0.3-0.5g/L carbon nanotube+90g/L sucrose+7.5g/L agar, will after inoculation
Material is put into culturing room and carries out normal condition dark culturing；(6) after cultivating 15d on culture medium five, by potato stem with bud
Embedding pearl is transferred to six: MS+0.3-0.5g/L graphene quantum dot+90g/L sucrose+7.5g/L agar of culture medium, will after inoculation
Material is put into culturing room and carries out normal condition dark culturing；(7) after cultivating 15d on culture medium six, by potato stem with bud
Embedding pearl is transferred to seven: MS+0.3-0.5g/L carbon nanotube+90g/L sucrose+7.5g/L agar of culture medium, by material after inoculation
It is put into culturing room and carries out normal condition dark culturing；(8) potato stem with bud embedding pearl normal condition on culture medium seven is black
After dark culture 60d, it is white potato shaped at Potato microtuber quantity it is more, diameter is big, effect is good.
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| CN201811654689.4A CN109673511A (en) | 2018-12-17 | 2018-12-17 | A method of improving test tube potato inducing effect |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713406A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Embedding vitrification ultra-low temperature detoxification method for Huaiyoshan high-mountain potatoes |
| CN111713407A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Method for embedding, transplanting and domesticating stem segments with buds of Huaiyoshan high-mountain potatoes |
| CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
| CN112753577A (en) * | 2021-01-14 | 2021-05-07 | 上饶师范学院 | Germination method of miniature hemp seed potato |
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| CN104026009A (en) * | 2014-05-23 | 2014-09-10 | 甘肃省农业科学院生物技术研究所 | Purple potato test tube mini-tuber induction method |
| CN108371101A (en) * | 2018-02-09 | 2018-08-07 | 中南民族大学 | A kind of the induction propagating culture medium and application process of potato son potato |
| CN108901843A (en) * | 2018-06-27 | 2018-11-30 | 上饶师范学院 | A method of improving radix tetrastigme root tuber in-vitro inducing effect |
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| CN104026009A (en) * | 2014-05-23 | 2014-09-10 | 甘肃省农业科学院生物技术研究所 | Purple potato test tube mini-tuber induction method |
| CN108371101A (en) * | 2018-02-09 | 2018-08-07 | 中南民族大学 | A kind of the induction propagating culture medium and application process of potato son potato |
| CN108901843A (en) * | 2018-06-27 | 2018-11-30 | 上饶师范学院 | A method of improving radix tetrastigme root tuber in-vitro inducing effect |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713406A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Embedding vitrification ultra-low temperature detoxification method for Huaiyoshan high-mountain potatoes |
| CN111713407A (en) * | 2020-06-30 | 2020-09-29 | 上饶师范学院 | Method for embedding, transplanting and domesticating stem segments with buds of Huaiyoshan high-mountain potatoes |
| CN111819952A (en) * | 2020-07-29 | 2020-10-27 | 上饶师范学院 | Embedding germination accelerating method for miniature Huaiyoshan high-mountain potatoes |
| CN112753577A (en) * | 2021-01-14 | 2021-05-07 | 上饶师范学院 | Germination method of miniature hemp seed potato |
| CN112753577B (en) * | 2021-01-14 | 2022-08-09 | 上饶师范学院 | Germination method of miniature hemp seed potato |
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