CN111771715A - Comprehensive technology for improving peanut hybridization efficiency - Google Patents

Comprehensive technology for improving peanut hybridization efficiency Download PDF

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Publication number
CN111771715A
CN111771715A CN202010687947.XA CN202010687947A CN111771715A CN 111771715 A CN111771715 A CN 111771715A CN 202010687947 A CN202010687947 A CN 202010687947A CN 111771715 A CN111771715 A CN 111771715A
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hybridization
planting
parent
petals
female parent
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李伟堂
刘红欣
牟书靓
杨翔宇
牛海龙
李玉发
何中国
王曙文
王佰众
何禹璇
李晓伟
李洋
任孝慈
徐冲
苏江顺
徐小龙
阴闯
张君
谭程友
王丹
靳一男
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Jilin Academy of Agricultural Sciences
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Jilin Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a comprehensive technology for improving peanut hybridization efficiency, which comprises the following steps: (1) constructing a hybridization pool: the hybridization tank adopts a brick-concrete structure, the bottom of the hybridization tank uses neutral sandy loam as a lower base layer, and the upper part is a planting layer; (2) planting the hybrid parents: coating the hybrid parent rice, and planting at the planting density of 38-42cm of the distance between the two parents, 22-28cm of the plant distance between the female parent and 13-18cm of the plant distance between the male parents; (3) management techniques before hybridization; (4) manually castrating the female parent; (5) artificial pollination; (6) and (3) comprehensive management after pollination: after all combined pollinations, 7: before 00, all non-cross pollinated flowers on the female parent plant are manually removed, the period lasts for 10 to 15 days, and proper manual hilling is carried out. The comprehensive technology for improving the peanut hybridization efficiency provided by the invention can promote the peanut hybridization maturing rate to be improved from the original 10% to about 18%, and obviously improves the breeding efficiency.

Description

Comprehensive technology for improving peanut hybridization efficiency
Technical Field
The invention belongs to the technical field of peanut hybridization, and particularly relates to a comprehensive technology for improving peanut hybridization efficiency.
Background
Peanut breeding can be divided into introduction, systematic breeding, crossbreeding, mutation breeding and biotechnology breeding. The cross breeding (the cross breeding is to use parents of different genotypes for cross breeding, make the filial generation of different variation types through gene recombination, and then breed a new variety through selective breeding) is the most common and most effective method applied at home and abroad. The offspring material obtained by artificial hybridization is the most direct and basic test material source for the new variety breeding research of peanuts. The artificial hybridization technology comprises the selection and matching of parents, the selection of a hybridization mode, the planting of parents and parents, the preparation of materials used for hybridization, emasculation, pollination, fruit needle burying, harvesting and the like. Mastering the corresponding artificial hybridization technology is the premise of making good the breeding of new peanut varieties. However, in recent years, the research on the field of peanut hybridization is not abundant, and less professionals are required to be skilled in manual peanut hybridization, so that the cost of peanut hybridization is continuously increased, the hybridization efficiency is low, the proportion of false hybrids is higher, and the development of peanut breeding and the peanut industry is seriously influenced.
Disclosure of Invention
The invention provides a scientific and reliable planting method for improving peanut hybridization efficiency aiming at the problems in the prior art.
The invention is realized by the following technical scheme.
The comprehensive technology for improving peanut hybridization efficiency comprises the following steps:
(1) construction of hybridization pools
The hybridization pond adopts a brick-concrete structure, the bottom of the hybridization pond uses neutral sandy loam as a lower base layer, the upper part of the hybridization pond is a planting layer, the planting layer is mixed with decomposed farmyard manure and special compound fertilizer for peanuts according to the proportion of 1m3Adding 6-12g of 25% wettable powder chlorothalonil and 45-55g of 5% toxin-pungent granules into soil, mixing, paving a drip irrigation pipe in the middle in the north-south direction to thoroughly irrigate a planting layer, and finally covering with a mulching film;
(2) planting of hybrid parents
Coating the hybrid parent rice, planting at a planting density of 38-42cm of the distance between the two parents, 22-28cm of the plant distance of the female parent and 13-18cm of the plant distance of the male parent, adopting a wood stick with one hemispherical end, pricking a mulching film at the hemispherical end of the wood stick by means of a meter ruler scale to form a hole with the depth of 2-3cm, sowing 2-4 grains in the hole, covering 8-12cm of soil on a seedling belt, and compacting by using smooth boards with equal width;
(3) pre-hybridization management techniques
Checking and filling seedlings after the parents sprout, exposing cotyledons as soon as possible by highland barley, receiving illumination, timely fixing single plants after the parent seedlings are aligned, pruning the main stem when the second opposite side branches of the main stem grow to 3-5cm, pruning the main stem at the position 0.4-0.6cm above the joint of the second opposite side branches and the main stem by using scissors during pruning, immediately spraying 25% carbendazim 450-doped 550 liquid to a pruning port for sterilization after the main stem is pruned, taking the combination as a unit, and 7: manually removing flowers which are opened before and after the initial flowering phase of the female parent every day before 00, or picking off buds which are about to be opened in the next day of female parent plants in the afternoon of the previous day until the end of hybridization;
(4) artificial castration of female parent
The artificial castration of the female parent is carried out when the female parent meets the flowering phase of the male parent after the female parent begins to bloom, and the castration time is generally 16 in the afternoon every day: after 00, removing the stamens, selecting buds with calyx microcracks and exposed yellow petals, pinching the bases of the buds by the thumb and the middle finger of the left hand during the castration, slightly poking the calyxes, the flag petals and the petals by the forceps held by the right hand, pressing the poked petals by the index finger and the thumb of the left hand, slightly pressing the back of the dragon petals by the forceps to expose the stamens, picking off the anthers of 8 stamens by the forceps for one or more times to remove the anthers, pushing the dragon petals back to the original position by the fingers to restore the original state of the flag petals and the petals, removing one flower from each inflorescence, and manually removing the rest buds with same position after the castration;
(5) artificial pollination
Before pollination, collecting pollen-dispersed male parent flowers according to the combined pollination amount, then extruding the pollen of the male parent by using tweezers, putting the pollen of the male parent into a culture dish sterilized by alcohol, when the pollination is carried out, holding the castrated flowers by using a left index finger and a middle finger, slightly pressing a keel slice by using a thumb of a hand or the tweezers to expose a pistil stigma, dipping the pollen on the stigma by using the tip of the tweezers for 1 time or more, and then restoring the keel slice to wrap the stigma;
(6) comprehensive management after pollination
After all combined pollinations, 7: before 00, all non-hybrid pollinated flowers on the female parent plant are manually removed, the period lasts for 10 to 15 days, proper manual hilling is carried out on the low-position pollinated flowers and fruit needles after pollination is finished, and the high-position pollinated flowers and fruit needles are buried in the soil in a mode of simultaneously pressing branches and hilling, and receive dark stimulation.
Specifically, in the step (2), the coating treatment of the hybrid parent rice is carried out three days before sowing, and the coating treatment liquid is prepared from the following components in parts by weight: 15-25 parts of 30% chlorpyrifos suspending agent, 11000 parts of 50% thiram 9000-containing water and 30000 parts of water 20000-containing water.
Specifically, in the step (1), the height of the hybridization tank is 80cm, the width of the tank is 80cm, the length of the hybridization tank is 1500cm, the height of the lower base layer is 40cm, and the height of the planting layer is 40 cm.
Specifically, the ratio of N to P to K in the special compound fertilizer for peanuts is 11: 16: 18.
specifically, in the step (5), the pollinated flower is a flower emasculated in the previous day.
Specifically, when the lateral branches of the later-stage plants exceed 35cm, uniconazole is sprayed to control the lateral branches to grow excessively, or the lateral branches are manually cut.
Specifically, after the lateral branches are manually cut, 25% carbendazim 500-time liquid, 25% azoxystrobin 1500-time liquid or 10% difenoconazole dispersible granule 3000-time liquid is used for spraying trimmed stems of the lateral branches and soil.
According to the technical scheme, the beneficial effects of the invention are as follows:
the comprehensive technology for improving the peanut hybridization efficiency provided by the invention is simple to operate, low in planting cost, capable of promoting the peanut hybridization maturing rate to be improved to about 18% from the original 10%, reducing false hybrids, good in hybrid plumpness, providing excellent materials for breeding and remarkably improving the breeding efficiency.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer.
Example 1
The comprehensive technology for improving peanut hybridization efficiency comprises the following steps:
(1) construction of hybridization pools
The hybridization pond adopts a brick-concrete structure, the bottom of the hybridization pond uses neutral sandy loam as a lower base layer, the upper part of the hybridization pond is a planting layer, the planting layer is mixed with decomposed farmyard manure and special compound fertilizer for peanuts according to the proportion of 1m3Adding 6g of 25% wettable powder chlorothalonil and 45g of 5% toxin-pungent granules into soil, mixing, paving a drip irrigation pipe in the middle of the soil in the north and south directions to thoroughly irrigate a planting layer, and finally covering the planting layer by using a mulching film, wherein the height of a hybridization tank is 80cm, the width of the tank is 80cm, the length of the tank is 1500cm, the height of a lower base layer is 40cm, the height of the planting layer is 40cm, and the N, P, K ratio in the special compound fertilizer for peanuts is 11: 16: 18;
(2) planting of hybrid parents
Coating the hybrid parent seed rice, then planting with the planting density of 38cm of biparental row spacing, 22cm of female parent plant spacing and 13cm of male parent plant spacing, adopting a wood stick with one hemispherical end, pricking a mulching film at the hemispherical end of the wood stick by means of a meter ruler scale to form a hole with the depth of 2cm, performing hole sowing for 2 grains, covering soil on a seedling belt with the width of 8cm and the height of 2cm, and compacting by using a smooth board with the same width, wherein the coating of the hybrid parent seed rice is treated three days before sowing, and the coating treatment liquid is prepared from the following components in parts by weight: 15 parts of 30% chlorpyrifos suspending agent, 9000 parts of 50% thiram and 20000 parts of water;
(3) pre-hybridization management techniques
Checking and filling seedlings after the parents sprout, exposing cotyledons as soon as possible by highland barley, receiving illumination, timely fixing a single plant after the maternal seedlings are aligned, pruning the main stem when the second opposite side branch of the main stem grows to 3cm, pruning the main stem at a position 0.4cm above the joint of the second opposite side branch and the main stem by using scissors, immediately spraying a prepared 25% carbendazim 450-time liquid to a pruning port for sterilization after pruning the main stem, and taking the combination as a unit, wherein 7: manually removing flowers which are opened before and after the initial flowering phase of the female parent every day before 00 till the end of hybridization;
(4) artificial castration of female parent
The artificial castration of the female parent is carried out when the female parent meets the flowering phase of the male parent after the female parent begins to bloom, and the castration time is generally 16 in the afternoon every day: after 00, removing the stamens, selecting buds with calyx microcracks and exposed yellow petals, pinching the bases of the buds by the thumb and the middle finger of the left hand during the castration, slightly poking the calyxes, the flag petals and the petals by the forceps held by the right hand, pressing the poked petals by the index finger and the thumb of the left hand, slightly pressing the back of the dragon petals by the forceps to expose the stamens, picking off the anthers of 8 stamens by the forceps for one or more times to remove the anthers, pushing the dragon petals back to the original position by the fingers to restore the original state of the flag petals and the petals, removing one flower from each inflorescence, and manually removing the rest buds with same position after the castration;
(5) artificial pollination
Before pollination, collecting pollen-dispersed male parent flowers according to the combined pollination amount, then extruding the male parent pollen by using tweezers, putting the pollen into a culture dish sterilized by using alcohol, when the pollination is carried out, holding the castrated flowers by using a left index finger and a middle finger, slightly pressing a keel slice by using a thumb of a hand or the tweezers to expose a pistil stigma, dipping the pollen on the stigma by using the tip of the tweezers for 1 time or more, then restoring the keel slice, and wrapping the stigma, wherein the pollinated flowers are castrated flowers in the previous day;
(6) comprehensive management after pollination
After all combined pollinations, 7: before 00, all non-hybrid pollinated flowers on the female parent plant are manually removed, the period lasts for 10 days, proper manual hilling is carried out on the lower pollination flowers and the fruit needles after pollination is finished, and the higher pollination flowers and the fruit needles are buried in the soil in a way of simultaneously pressing branches and hilling, and receive dark stimulation. And when the later-stage plant lateral branches exceed 35cm, spraying uniconazole to control over-vigorous growth of the lateral branches, or manually cutting the lateral branches, wherein after the lateral branches are manually cut, spraying 500 times of 25% carbendazim solution on the cut lateral branch stems and soil.
Example 2
The comprehensive technology for improving peanut hybridization efficiency comprises the following steps:
(1) construction of hybridization pools
The hybridization tank adopts a brick-concrete structure and the bottom of the hybridization tankThe part uses neutral sandy loam as a lower base layer, the upper part is a planting layer, and the planting layer is mixed with decomposed farmyard manure and special compound fertilizer for peanuts according to the proportion of 1m3Adding 8g of 25% wettable powder chlorothalonil and 50g of 5% toxin-pungent granules into soil, mixing, paving a drip irrigation pipe in the middle of the soil in the north and south directions to thoroughly irrigate a planting layer, and finally covering the planting layer by using a mulching film, wherein the height of a hybridization tank is 80cm, the width of the tank is 80cm, the length of the tank is 1500cm, the height of a lower base layer is 40cm, the height of the planting layer is 40cm, and the N, P, K ratio in the special compound fertilizer for peanuts is 11: 16: 18;
(2) planting of hybrid parents
Coating the hybrid parent seed rice, then planting with the planting density of 40cm of distance between two parents, 24cm of distance between female parent plants and 15cm of distance between male parent plants, adopting a wood stick with one hemispherical end, pricking a mulching film at the hemispherical end of the wood stick by means of a meter ruler scale to form a hole with the depth of 2.5cm, performing hole sowing for 3 grains, covering soil on a seedling belt with the width of 10cm and the height of 2.5cm, and compacting by using a smooth board with the same width, wherein the coating of the hybrid parent seed rice is treated three days before sowing, and the coating treatment liquid is prepared from the following components in parts by weight: 20 parts of 30% chlorpyrifos suspending agent, 10000 parts of 50% thiram and 25000 parts of water;
(3) pre-hybridization management techniques
Checking and filling seedlings after the parents sprout, exposing cotyledons as soon as possible by highland barley, receiving illumination, timely fixing a single plant after the maternal seedlings are aligned, pruning the main stem when the second opposite side branch of the main stem grows to 4cm, pruning the main stem at a position 0.5cm above the joint of the second opposite side branch and the main stem by using scissors, immediately spraying a prepared 25% carbendazim 500-fold liquid to a pruning port for sterilization after the main stem is pruned, taking the combination as a unit, and 7: manually removing flowers which are opened before and after the initial flowering phase of the female parent every day before 00 till the end of hybridization;
(4) artificial castration of female parent
The artificial castration of the female parent is carried out when the female parent meets the flowering phase of the male parent after the female parent begins to bloom, and the castration time is generally 16 in the afternoon every day: after 00, removing the stamens, selecting buds with calyx microcracks and exposed yellow petals, pinching the bases of the buds by the thumb and the middle finger of the left hand during the castration, slightly poking the calyxes, the flag petals and the petals by the forceps held by the right hand, pressing the poked petals by the index finger and the thumb of the left hand, slightly pressing the back of the dragon petals by the forceps to expose the stamens, picking off the anthers of 8 stamens by the forceps for one or more times to remove the anthers, pushing the dragon petals back to the original position by the fingers to restore the original state of the flag petals and the petals, removing one flower from each inflorescence, and manually removing the rest buds with same position after the castration;
(5) artificial pollination
Before pollination, collecting pollen-dispersed male parent flowers according to the combined pollination amount, then extruding the male parent pollen by using tweezers, putting the pollen into a culture dish sterilized by using alcohol, when the pollination is carried out, holding the castrated flowers by using a left index finger and a middle finger, slightly pressing a keel slice by using a thumb of a hand or the tweezers to expose a pistil stigma, dipping the pollen on the stigma by using the tip of the tweezers for 1 time or more, then restoring the keel slice, and wrapping the stigma, wherein the pollinated flowers are castrated flowers in the previous day;
(6) comprehensive management after pollination
After all combined pollinations, 7: and (5) manually removing all non-hybrid pollinated flowers on the female parent plant before 00 days, continuing for 13 days, carrying out proper manual hilling on the lower pollination flowers and fruit needles after pollination is finished, and burying the higher pollination flowers and fruit needles in the soil in a way of simultaneously pressing branches and hilling to receive dark stimulation. And when the later-stage plant lateral branches exceed 35cm, spraying uniconazole to control over-vigorous growth of the lateral branches, or manually cutting the lateral branches, wherein after the lateral branches are manually cut, spraying 1500 times of 25% azoxystrobin solution on the cut lateral branch stems and soil.
Example 3
The comprehensive technology for improving peanut hybridization efficiency comprises the following steps:
(1) construction of hybridization pools
The hybridization pond adopts a brick-concrete structure, the bottom of the hybridization pond uses neutral sandy loam as a lower base layer, the upper part of the hybridization pond is a planting layer, the planting layer is mixed with decomposed farmyard manure and special compound fertilizer for peanuts according to the proportion of 1m3Adding 12g of 25% wettable pulvis chlorothalonil and 55g of 5% toxin-pungent granules into soil, mixing, paving a drip irrigation pipe in the middle of the soil in the north and south directions to thoroughly irrigate a planting layer, and finally covering the planting layer with a mulching film, wherein the height of a hybridization pool is 80cm, and the width of the pool is 80cmcm, length of 1500cm, lower base layer height of 40cm, planting layer height of 40cm, and N, P and K ratio in the special compound fertilizer for peanuts of 11: 16: 18;
(2) planting of hybrid parents
Coating hybrid parent seed rice, planting with a planting density of 42cm of biparental row spacing, 28cm of female parent plant spacing and 18cm of male parent plant spacing, adopting a wood stick with one hemispherical end, pricking a mulching film at the hemispherical end of the wood stick by means of a meter ruler scale to form a hole with the depth of 3cm, performing hole sowing for 4 grains, covering soil on a seedling belt with the width of 12cm and the height of 3cm, and compacting by using a smooth board with the same width, wherein the coating of the hybrid parent seed rice is treated three days before sowing, and a coating treatment liquid is prepared from the following components in parts by weight: 25 parts of 30% chlorpyrifos suspending agent, 11000 parts of 50% thiram and 30000 parts of water;
(3) pre-hybridization management techniques
Checking and supplementing seedlings after the parents emerge, exposing cotyledons as soon as possible by highland barley, receiving illumination, timely fixing a single plant after the maternal seedlings are aligned, pruning a main stem when a second pair of lateral branches of the main stem grow to 5cm, pruning the main stem at a position 0.6cm above the joint of the second pair of lateral branches and the main stem by using scissors during pruning, immediately spraying a pruning port by using prepared 25% carbendazim 550 times liquid for sterilization after the main stem is pruned, picking off a flower bud which is about to open next day of the maternal plant by taking the combination as a unit until hybridization is finished;
(4) artificial castration of female parent
The artificial castration of the female parent is carried out when the female parent meets the flowering phase of the male parent after the female parent begins to bloom, and the castration time is generally 16 in the afternoon every day: after 00, removing the stamens, selecting buds with calyx microcracks and exposed yellow petals, pinching the bases of the buds by the thumb and the middle finger of the left hand during the castration, slightly poking the calyxes, the flag petals and the petals by the forceps held by the right hand, pressing the poked petals by the index finger and the thumb of the left hand, slightly pressing the back of the dragon petals by the forceps to expose the stamens, picking off the anthers of 8 stamens by the forceps for one or more times to remove the anthers, pushing the dragon petals back to the original position by the fingers to restore the original state of the flag petals and the petals, removing one flower from each inflorescence, and manually removing the rest buds with same position after the castration;
(5) artificial pollination
Before pollination, collecting pollen-dispersed male parent flowers according to the combined pollination amount, then extruding the male parent pollen by using tweezers, putting the pollen into a culture dish sterilized by using alcohol, when the pollination is carried out, holding the castrated flowers by using a left index finger and a middle finger, slightly pressing a keel slice by using a thumb of a hand or the tweezers to expose a pistil stigma, dipping the pollen on the stigma by using the tip of the tweezers for 1 time or more, then restoring the keel slice, and wrapping the stigma, wherein the pollinated flowers are castrated flowers in the previous day;
(6) comprehensive management after pollination
After all combined pollinations, 7: removing all non-hybrid pollinated flowers from the female parent plant before 00 days, continuing for 15 days, carrying out proper artificial hilling on the lower pollination flowers and fruit needles after pollination, burying the higher pollination flowers and fruit needles in soil in a way of simultaneously pressing branches and hilling, and receiving dark stimulation. And when the later-stage plant lateral branches exceed 35cm, spraying uniconazole to control over-vigorous growth of the lateral branches, or manually cutting the lateral branches, wherein 3000 times of liquid of 10% difenoconazole dispersible granules is used for spraying trimmed lateral branch stems and soil after the lateral branches are manually cut.
Peanut hybridization tests were performed by the methods of the examples, and then the bearing rate of each group of peanut hybridization was counted, and the test results are shown in table 1:
TABLE 1 peanut hybrid seed set percentage
Figure BDA0002588257840000071
Figure BDA0002588257840000081
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims (7)

1. The comprehensive technology for improving peanut hybridization efficiency is characterized by comprising the following steps of:
(1) construction of hybridization pools
The hybridization pond adopts a brick-concrete structure, the bottom of the hybridization pond uses neutral sandy loam as a lower base layer, the upper part of the hybridization pond is a planting layer, the planting layer is mixed with decomposed farmyard manure and special compound fertilizer for peanuts according to the proportion of 1m3Adding 6-12g of 25% wettable powder chlorothalonil and 45-55g of 5% toxin-pungent granules into soil, mixing, paving a drip irrigation pipe in the middle in the north-south direction to thoroughly irrigate a planting layer, and finally covering with a mulching film;
(2) planting of hybrid parents
Coating the hybrid parent rice, planting at a planting density of 38-42cm of the distance between the two parents, 22-28cm of the plant distance of the female parent and 13-18cm of the plant distance of the male parent, adopting a wood stick with one hemispherical end, pricking a mulching film at the hemispherical end of the wood stick by means of a meter ruler scale to form a hole with the depth of 2-3cm, sowing 2-4 grains in the hole, covering 8-12cm of soil on a seedling belt, and compacting by using smooth boards with equal width;
(3) pre-hybridization management techniques
Checking and filling seedlings after the parents sprout, exposing cotyledons as soon as possible by highland barley, receiving illumination, timely fixing single plants after the parent seedlings are aligned, pruning the main stem when the second opposite side branches of the main stem grow to 3-5cm, pruning the main stem at the position 0.4-0.6cm above the joint of the second opposite side branches and the main stem by using scissors during pruning, immediately spraying 25% carbendazim 450-doped 550 liquid to a pruning port for sterilization after the main stem is pruned, taking the combination as a unit, and 7: manually removing flowers which are opened before and after the initial flowering phase of the female parent every day before 00, or picking off buds which are about to be opened in the next day of female parent plants in the afternoon of the previous day until the end of hybridization;
(4) artificial castration of female parent
The artificial castration of the female parent is carried out when the female parent meets the flowering phase of the male parent after the female parent begins to bloom, and the castration time is generally 16 in the afternoon every day: after 00, removing the stamens, selecting buds with calyx microcracks and exposed yellow petals, pinching the bases of the buds by the thumb and the middle finger of the left hand during the castration, slightly poking the calyxes, the flag petals and the petals by the forceps held by the right hand, pressing the poked petals by the index finger and the thumb of the left hand, slightly pressing the back of the dragon petals by the forceps to expose the stamens, picking off the anthers of 8 stamens by the forceps for one or more times to remove the anthers, pushing the dragon petals back to the original position by the fingers to restore the original state of the flag petals and the petals, removing one flower from each inflorescence, and manually removing the rest buds with same position after the castration;
(5) artificial pollination
Before pollination, collecting pollen-dispersed male parent flowers according to the combined pollination amount, then extruding the pollen of the male parent by using tweezers, putting the pollen of the male parent into a culture dish sterilized by alcohol, when the pollination is carried out, holding the castrated flowers by using a left index finger and a middle finger, slightly pressing a keel slice by using a thumb of a hand or the tweezers to expose a pistil stigma, dipping the pollen on the stigma by using the tip of the tweezers for 1 time or more, and then restoring the keel slice to wrap the stigma;
(6) comprehensive management after pollination
After all combined pollinations, 7: before 00, all non-hybrid pollinated flowers on the female parent plant are manually removed, the period lasts for 10 to 15 days, proper manual hilling is carried out on the low-position pollinated flowers and fruit needles after pollination is finished, and the high-position pollinated flowers and fruit needles are buried in the soil in a mode of simultaneously pressing branches and hilling, and receive dark stimulation.
2. The comprehensive technology for improving the hybridization efficiency of peanuts according to claim 1, wherein in the step (2), the coating treatment is carried out on the hybrid parent seeds three days before sowing, and the coating treatment liquid is prepared from the following components in parts by weight: 15-25 parts of 30% chlorpyrifos suspending agent, 11000 parts of 50% thiram 9000-containing water and 30000 parts of water 20000-containing water.
3. The comprehensive technology for improving the peanut hybridization efficiency according to claim 1, characterized in that in the step (1), the hybridization tank is 80cm high, the tank is 80cm wide and 1500cm long, the lower base layer is 40cm high, and the planting layer is 40cm high.
4. The comprehensive technology for improving peanut hybridization efficiency according to claim 1, wherein the ratio of N to P to K in the special compound fertilizer for peanuts is 11: 16: 18.
5. the integrated technique for improving peanut hybridization efficiency as claimed in claim 1, wherein in the step (5), the pollinated flower is a castrated flower of the previous day.
6. The comprehensive technology for improving peanut hybridization efficiency according to claim 1, wherein after the lateral branches of the later-stage plants exceed 35cm, uniconazole is sprayed to control the lateral branches to grow excessively, or manual lateral branch pruning is adopted.
7. The comprehensive technology for improving the peanut hybridization efficiency according to claim 6, characterized in that 25% carbendazim 500-fold liquid, 25% azoxystrobin 1500-fold liquid or 10% difenoconazole dispersible granule 3000-fold liquid is used for spraying the trimmed side branch stems and soil after the side branches are manually cut.
CN202010687947.XA 2020-07-16 2020-07-16 Comprehensive technology for improving peanut hybridization efficiency Pending CN111771715A (en)

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