CN111714439A

CN111714439A - Anti-wrinkle essence powder and preparation method thereof

Info

Publication number: CN111714439A
Application number: CN202010528811.4A
Authority: CN
Inventors: 胡勇
Original assignee: Shanghai Benzheng Bioengineering Co ltd
Priority date: 2020-06-11
Filing date: 2020-06-11
Publication date: 2020-09-29

Abstract

The invention relates to anti-wrinkle essence powder and a preparation method thereof, the essence powder is prepared by adopting trehalose, a leukocyte extract, rice fermentation liquor, a motherwort extract, a flos gynurae extract, a gynostemma pentaphyllum extract, hexapeptide and an apple stem cell stock solution as raw materials and carrying out proper weight proportion, and the prepared essence powder has excellent penetrating capacity and bioactivity, can be absorbed through skin and deeply permeate, delays the generation of skin wrinkles and enables the skin to keep elasticity and fineness. The essence powder disclosed by the invention is scientific and reasonable in compatibility, can effectively resist skin aging, is safe and effective, and has no side effect.

Description

Anti-wrinkle essence powder and preparation method thereof

Technical Field

The invention belongs to the technical field of skin care products, and particularly relates to anti-wrinkle essence powder and a preparation method thereof.

Background

When the rate of cell regeneration is lower than the rate of apoptosis, the skin of a human begins to have aging phenomena such as wrinkles, color spots and the like, and the irreversibility of aging causes many women to feel dysphoria. Beauty-conscious, especially for women, and aging is the negative of beauty, so no girls would like aging, and wrinkle growth is the onset of aging for many people. Therefore, a lot of anti-wrinkle technologies or anti-wrinkle skin care products appear in the existing market, but although the traditional skin-pulling operation can achieve the effect immediately, people always feel unnatural stiffness, and most of the existing anti-wrinkle skin care products are expensive and have unsatisfactory effects. Therefore, a product with high cost performance and obvious anti-wrinkle effect is urgently needed in the existing market.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides anti-wrinkle essence powder and a preparation method thereof. The essence powder has excellent penetrating ability and bioactivity, can be absorbed through skin, can deeply permeate, delays the generation of skin wrinkles, and keeps the skin elastic and fine.

The technical scheme adopted by the invention is as follows:

an anti-wrinkle essence powder comprises the following raw material components:

1-2 parts of trehalose;

5-8 parts of rice fermentation liquor;

0.2-0.8 parts by weight of leukocyte extract;

2-4 parts of motherwort extract;

2-4 parts of flos chrysanthemi indici extract;

2-4 parts of gynostemma pentaphylla extract;

0.1-0.3 weight portion of hexapeptide;

0.1-0.3 parts of apple stem cell stock solution.

Further, the anti-wrinkle essence powder comprises the following raw material components:

1.5 parts by weight of trehalose;

7.5 parts of rice fermentation liquor;

0.5 weight portion of leucocyte extract;

3 parts of motherwort extract;

3 parts of flos chrysanthemi indici extract;

3 parts of gynostemma pentaphylla extract;

0.2 weight portion of hexapeptide;

0.2 part of apple stem cell stock solution.

The leucocyte extract in the anti-wrinkle essence powder is prepared by adopting the following method:

(1) taking human venous blood, adding an anticoagulant, and slightly and uniformly mixing to obtain diluted blood;

(2) standing the diluted blood, dividing the diluted blood into three layers, and sucking a middle cell layer to obtain cell sap to be treated;

(3) adding balanced salt solution into the cell sap to be treated, uniformly mixing, centrifuging, and then discarding supernatant to obtain precipitated cells for amplification, thereby obtaining amplified white blood cells and an amplification culture solution;

(4) extracting nucleotide from the amplified white blood cells, extracting protein from the amplification culture solution, and mixing the extracted nucleotide and the extracted protein to obtain the white blood cell extract.

Further, in the step (1), the anticoagulant is ethylenediamine tetraacetic acid or sodium oxalate, and the concentration of the anticoagulant in blood is 4.45 ± 0.85 μmol.

Further, in the step (2), the standing temperature is 37 ℃, and the standing time is 50-70 min.

Further, in the step (3), the balanced salt solution is Ca-free²⁺、Mg²⁺The centrifugation speed of the Hank's solution is 1500-2500r/min, the centrifugation time is 5-15min, and the amplification is carried out by using RPMI1640 culture medium containing 5% FBS and 200U of interleukin 2.

Further, in the step (4), DNAzol is used for extracting the nucleotide in the white blood cells after the amplification^TMReagent kit; the method for extracting and amplifying the protein in the culture solution comprises the following steps: and filtering the amplification culture solution through a 0.22 mu m filter membrane to obtain the protein.

Further, the obtained leukocyte extract contains basic fibroblast growth factor, interleukin 2, interleukin 4 and interferon gamma.

The rice fermentation liquor is prepared by adopting the following method:

(B1) soaking rice in water, and steaming to obtain cooked rice;

(B2) inoculating Aspergillus oryzae into the cooked rice, wherein the inoculation mass of the Aspergillus oryzae accounts for 0.5-0.9% of the cooked rice, culturing at 36-38 deg.C and 80-88% humidity for 5-7 days, centrifuging, and filtering to obtain the rice fermentation liquid.

The motherwort extract is prepared by the following method:

(C1) drying and crushing motherwort to obtain fine motherwort powder;

(C2) adding distilled water into the motherwort fine powder obtained in the step (C1) for the first time, soaking for 50-60min, performing reflux extraction at 80-85 ℃ for 2-4h, centrifuging, and filtering to obtain a first filtrate and a first filter residue; adding distilled water into the first filter residue for the second time, performing heat preservation extraction at 75-80 ℃ for 2-4h, centrifuging, and filtering to obtain a second filtrate and a second filter residue; and combining the first filtrate and the second filtrate, concentrating to remove distilled water, and performing microporous filtration to obtain the motherwort herb extract.

Further, in the step (C1), the particle size of the motherwort fine powder is 50 to 60 mesh.

Further, in the step (C2), the mass ratio of the motherwort fine powder to the first addition of distilled water is 1:10-1: 15; the mass ratio of the first filter residue to the second addition of distilled water is 1:10-1: 15; the concentration is water bath concentration, the concentration temperature is 80-90 ℃, and the aperture of a filter membrane for carrying out the microporous filtration is 0.4-0.5 mu m.

Further, the preparation method of the flos chrysanthemi extract and the gynostemma pentaphyllum extract is the same as that of the motherwort extract.

The preparation method of the anti-wrinkle essence powder comprises the following specific steps:

respectively and uniformly mixing the trehalose, the leukocyte extract, the rice fermentation liquor, the motherwort extract, the flos chrysanthemi extract, the gynostemma pentaphyllum extract, the hexapeptide and the apple stem cell stock solution in parts by weight, and freeze-drying to obtain the essence powder.

Further, the mixing temperature is 10-20 ℃.

The leukocyte extract, the rice fermentation liquor or the flos caryophylli extract in the raw material components have synergistic anti-wrinkle effect, and the anti-wrinkle effect is greatly reduced if any raw material is absent.

The method is suitable for people: is suitable for any skin (sensitive skin is used after test), and is suitable for skin around eyes.

The using method comprises the following steps: tearing and pulling the bottle cap in the direction indicated by the arrow of the bottle cap, opening the rubber plug, spraying 2 ml of essence water developed by the inventor into the bottle, slightly shaking for dissolving, then unscrewing the nozzle of the essence bottle developed by the inventor, pouring the dissolved essence powder solution into the bottle, screwing the nozzle, shaking the bottle body up and down, and fully mixing. Storing at room temperature, and finishing within three months after preparation.

Note that: please place the infant in a position where the infant cannot get the infant; if the patient is uncomfortable after the use, the patient should pause the use and consult. If the product is carelessly dropped into the eye, please wash with clean water

The trehalose has strong moisturizing effect and physiological effects in various aspects such as sun protection, ultraviolet protection and the like, can be used as a humectant, a protective agent and the like to be added into emulsion, facial mask, essence, facial cleanser, and can also be used as a sweetening agent and a quality improver of lipstick, oral cleanser, oral aromatic and the like. The anhydrous trehalose can also be used in cosmetics as a dehydrating agent for phospholipids and enzymes, and the fatty acid derivatives thereof are also excellent surfactants.

The rice fermentation liquor is the main component of cuticle moisturizing factor NMF and is rich in natural amino acid. The amino acid molecules are one third of collagen molecules, can quickly supplement water to the cuticle, go deep into the skin, effectively moisturize and replenish water.

The leukocyte extract contains basic fibroblast growth factor, interleukin 2, interleukin 4 and gamma interferon, and has strong deep repairing function and sterilizing and inflammation diminishing functions on skin. When the white blood cells are subjected to amplification culture in vitro, a large amount of growth factors can be generated, so that the white blood cells and the culture solution are respectively extracted after the white blood cells are subjected to amplification culture, and a large amount of nucleotides and growth factors can be enriched and obtained; the leucocyte extract can perform synergistic action with other components in a cosmetic formula, synergistically improve the function of cell mitochondria, provide comprehensive nutrients for cell metabolism and obviously improve the function of activating cells of the leucocyte extract; the leukocyte extract has obvious functions of activating senescent cells, promoting cell regeneration, promoting collagen synthesis, repairing inflammatory injury and the like.

Herba Leonuri extract containing leonurine about 0.05%, stachydrine, rutin, fumaric acid (fumaric acid), leonuridine, 4-guanidinobutanol, 4-guanidinobutyric acid, arginine, amyrin, stigmasterol and sitosterol; 5 crystalline substances: leonurine A, leonurine B, leonurine A, leonurine B and leonurine C, and the leonurus extract can effectively diminish inflammation, whiten skin, beautify skin and resist aging.

Flos Lagerstroemiae Indicae extract can significantly inhibit melanin formation by inhibiting 1L-1a (inflammatory factor) and reducing NO (nitric oxide) production; has antioxidant effect and can scavenge free radicals; inhibiting the transport of melanosomes from melanocytes to keratinocytes; weak cells are repaired, red blood filaments are cleared up, skin tolerance is enhanced, the skin is far away from the problems of stabbing pain, desquamation, red swelling, roughness and the like, and skin allergy symptoms are improved from the root.

The main effective components of gynostemma pentaphylla are gypenoside, gypenoside (polysaccharide), water-soluble amino acid, flavonoid, multiple vitamins, trace elements, mineral substances and the like; the gynostemma pentaphylla extract contains rich saponin and can effectively resist senility and eliminate spots.

The hexapeptide can improve cell metabolism and smooth fine wrinkles of eyes, forehead, cheeks, neck and other parts; the integrity of cell tissues can be protected, the growth of collagen is promoted, and the skin is rich in luster and elasticity; can inhibit nerve conduction, avoid excessive muscular atrophy and fundamentally prevent wrinkles.

Apple stem cell stock solution, which helps skin stem cells maintain their properties and their ability to reconstitute skin tissue; it has antiaging and wrinkle removing effects. It is rich in plant nutrients, proteins and longevity cells (including stem cells with special longevity); these particular components give them excellent storage and longevity characteristics. Is the first plant stem cell active matter raw material which is verified to be effective on human skin stem cells in the market.

The invention has the beneficial effects that:

the anti-wrinkle essence powder disclosed by the invention is prepared from the raw materials of trehalose, a leukocyte extract, rice fermentation liquor, a motherwort extract, a flos chrysanthemi extract, a gynostemma pentaphylla extract, hexapeptide and an apple stem cell stock solution in a proper weight ratio, and has excellent penetrating capacity and bioactivity, can be absorbed through a skin and deeply permeates, and can delay the generation of skin wrinkles and keep the skin elastic and fine.

The trehalose has strong moisturizing effect and physiological effects of sun protection, ultraviolet protection and the like; the rice fermentation liquor is the main component of the cuticle moisturizing factor NMF and is rich in natural amino acid. The amino acid molecules are one third of collagen molecules, can quickly supplement water to the horny layer, go deep into the skin, effectively moisturize and replenish water; the leucocyte extract provides comprehensive nutrients for cell metabolism, obviously improves the cell activation function of the leucocyte extract, has a strong deep repair function, and has obvious functions of activating senescent cells, promoting cell regeneration, promoting collagen synthesis, repairing inflammatory injury and the like; the motherwort herb extract, the flos chrysanthemi extract and the gynostemma pentaphylla extract can effectively achieve the effects of resisting wrinkles, diminishing inflammation and whitening; the hexapeptide effectively improves the metabolism of cells, promotes the growth of collagen, inhibits nerve conduction and further prevents wrinkles; the apple stem cell stock solution helps skin stem cells maintain the characteristics and the capacity of reconstructing skin tissues, and can delay senility and remove wrinkles. The essence powder disclosed by the invention is reasonably compatible in science, has a synergistic effect of all components, can effectively resist skin aging, is safe and effective, and has no side effect.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

In the following examples 1mg is represented by 1 part by weight.

Example 1

The embodiment provides an anti-wrinkle essence powder, which comprises the following raw material components:

1 part by weight of trehalose;

5 parts of rice fermentation liquor;

0.2 parts by weight of leukocyte extract;

2 parts of motherwort extract;

2 parts of flos chrysanthemi indici extract;

2 parts of gynostemma pentaphylla extract;

0.1 part of hexapeptide;

0.1 part of apple stem cell stock solution.

Further, the preparation method of the anti-wrinkle essence powder comprises the following specific steps:

(1) preparing a leukocyte extract:

(A1) adding ethylene diamine tetraacetic acid or sodium oxalate into human venous blood, and gently mixing uniformly, wherein the concentration of the ethylene diamine tetraacetic acid or the sodium oxalate in the blood is 3.6 mu mol, so as to obtain diluted blood;

(A2) standing the diluted blood at 37 ℃ for 50min, dividing the diluted blood into three layers, and sucking a middle cell layer to obtain cell sap to be treated;

(A3) adding Ca-free cell sap to the cell sap to be treated²⁺、Mg²⁺Uniformly mixing the Hank's balanced salt solution, centrifuging at 1500r/min for 15min, and then discarding the supernatant, wherein the obtained precipitated cells are amplified by using an RPMI1640 culture medium containing 5% FBS and 200U of interleukin 2, so as to obtain the amplified white blood cells and an amplification culture solution;

(A4) using DNAzol^TMExtracting the nucleotide in the amplified leucocyte by using the Reagent kit, filtering the amplification culture solution by using a 0.22 mu m filter membrane to extract protein, and mixing the extracted nucleotide and the extracted protein to obtain the leucocyte extract;

(2) preparing rice fermentation liquor:

(B1) soaking rice in water, and steaming to obtain cooked rice;

(B2) inoculating aspergillus oryzae into the cooked rice, wherein the inoculation mass of the aspergillus oryzae accounts for 0.5 percent of the mass of the cooked rice, then culturing for 7 days under the conditions that the temperature is 36 ℃ and the humidity is 80 percent, and obtaining the rice fermentation liquor through centrifugation and filtration.

(3) Preparing a motherwort extract, a flos chrysanthemi extract and a gynostemma pentaphyllum extract:

(C1) respectively drying and crushing motherwort, flos chrysanthemi and gynostemma pentaphylla to obtain raw material fine powder with the particle size of 50 meshes;

(C2) adding distilled water which is 10 times of the weight of each raw material into the fine powder of each raw material in the step (C1) for the first time, soaking for 60min, performing reflux extraction at 80 ℃ for 4h, centrifuging, and filtering to obtain a first filtrate and a first filter residue of each raw material; adding distilled water with the mass 10 times of that of the first filter residue of each raw material into the first filter residue of each raw material for the second time, performing heat preservation extraction at 75 ℃ for 4 hours, centrifuging, and filtering to obtain a second filtrate and a second filter residue of each raw material; mixing the first filtrate and the second filtrate, concentrating in 80 deg.C water bath to remove distilled water, and performing microporous filtration with filter membrane aperture of 0.4 μm to obtain herba Leonuri extract.

(4) Respectively and uniformly mixing the trehalose, the leukocyte extract, the rice fermentation liquor, the motherwort extract, the flos chrysanthemi extract, the gynostemma pentaphyllum extract, the hexapeptide and the apple stem cell stock solution in parts by weight at 10-20 ℃, and freeze-drying to obtain the essence powder.

Example 2

2 parts of trehalose;

8 parts of rice fermentation liquor;

0.8 weight portion of leucocyte extract;

4 parts of motherwort extract;

4 parts of flos chrysanthemi indici extract;

4 parts of gynostemma pentaphylla extract;

0.3 part of hexapeptide;

0.3 part of apple stem cell stock solution.

(1) preparing a leukocyte extract:

(A1) adding ethylene diamine tetraacetic acid or sodium oxalate into human venous blood, and gently mixing uniformly, wherein the concentration of the ethylene diamine tetraacetic acid or the sodium oxalate in the blood is 5.3 mu mol, so as to obtain diluted blood;

(A2) standing the diluted blood at 37 ℃ for 70min, dividing the diluted blood into three layers, and sucking a middle cell layer to obtain cell sap to be treated;

(A3) adding Ca-free cell sap to the cell sap to be treated²⁺、Mg²⁺Uniformly mixing the Hank's balanced salt solution, centrifuging at 2500r/min for 5min, and then discarding the supernatant, wherein the obtained precipitated cells are amplified by using an RPMI1640 culture medium containing 5% FBS and 200U of interleukin 2, so as to obtain the amplified white blood cells and an amplification culture solution;

(2) preparing rice fermentation liquor:

(B1) soaking rice in water, and steaming to obtain cooked rice;

(B2) inoculating aspergillus oryzae into the cooked rice, wherein the inoculation mass of the aspergillus oryzae accounts for 0.9% of the mass of the cooked rice, then culturing for 5 days under the conditions that the temperature is 38 ℃ and the humidity is 88%, and centrifuging and filtering to obtain the rice fermentation liquor.

(C1) respectively drying and pulverizing herba Leonuri, flos Celosiae Cristatae and herba Gynostemmatis to obtain fine powder of 50-60 mesh;

(C2) adding distilled water 15 times the mass of each raw material into the fine powder of each raw material in the step (C1) for the first time, soaking for 50min, performing reflux extraction at 85 ℃ for 2h, centrifuging, and filtering to obtain a first filtrate and a first filter residue of each raw material; adding distilled water which is 15 times of the mass of the first filter residue of each raw material into the first filter residue of each raw material for the second time, performing heat preservation extraction at 80 ℃ for 2 hours, centrifuging, and filtering to obtain a second filtrate and a second filter residue of each raw material; and (3) combining the first filtrate and the second filtrate of the raw materials, concentrating in a water bath at 90 ℃ to remove distilled water, and then carrying out microfiltration, wherein the aperture of a filter membrane is 0.5 mu m, so as to obtain the leonurus extract.

(4) Respectively and uniformly mixing the trehalose, the leukocyte extract, the rice fermentation liquor, the motherwort extract, the flos chrysanthemi extract, the gynostemma pentaphyllum extract, the hexapeptide and the apple stem cell stock solution in parts by weight at 20 ℃, and freeze-drying to obtain the essence powder.

Example 3

1.5 parts by weight of trehalose;

7.5 parts of rice fermentation liquor;

0.5 weight portion of leucocyte extract;

3 parts of motherwort extract;

3 parts of flos chrysanthemi indici extract;

3 parts of gynostemma pentaphylla extract;

0.2 weight portion of hexapeptide;

0.2 part of apple stem cell stock solution.

(1) preparing a leukocyte extract:

(A1) adding ethylene diamine tetraacetic acid or sodium oxalate into human venous blood, and gently mixing uniformly, wherein the concentration of the ethylene diamine tetraacetic acid or the sodium oxalate in the blood is 4.45 mu mol, so as to obtain diluted blood;

(A2) standing the diluted blood at 37 ℃ for 60min, dividing the diluted blood into three layers, and sucking a middle cell layer to obtain cell sap to be treated;

(A3) into the cell fluid to be treatedAdding Ca-free²⁺、Mg²⁺Uniformly mixing the Hank's balanced salt solution, centrifuging at 2000r/min for 10min, and then discarding the supernatant, wherein the obtained precipitated cells are amplified by using an RPMI1640 culture medium containing 5% FBS and 200U of interleukin 2, so as to obtain the amplified white blood cells and an amplification culture solution;

(2) preparing rice fermentation liquor:

(B1) soaking rice in water, and steaming to obtain cooked rice;

(B2) inoculating aspergillus oryzae into the cooked rice, wherein the inoculation mass of the aspergillus oryzae accounts for 0.7% of the mass of the cooked rice, then culturing for 6 days under the conditions that the temperature is 37 ℃ and the humidity is 84%, and centrifuging and filtering to obtain the rice fermentation liquor.

(C1) respectively drying and crushing motherwort, flos chrysanthemi and gynostemma pentaphylla to obtain raw material fine powder with the particle size of 55 meshes;

(C2) adding distilled water which is 10-15 times of the weight of each raw material into each raw material fine powder in the step (C1) for the first time, soaking for 55min, performing reflux extraction at 83 ℃ for 3h, centrifuging, and filtering to obtain a first filtrate and a first filter residue of each raw material; adding distilled water 13 times of the mass of the first raw material filter residue into the first raw material filter residue for the second time, performing heat preservation extraction at 77 ℃ for 3 hours, centrifuging, and filtering to obtain a second raw material filtrate and a second filter residue; mixing the first filtrate and the second filtrate, concentrating in 85 deg.C water bath to remove distilled water, and performing microporous filtration with filter membrane pore diameter of 0.4 μm to obtain herba Leonuri extract.

(4) Respectively and uniformly mixing the trehalose, the leukocyte extract, the rice fermentation liquor, the motherwort extract, the flos chrysanthemi extract, the gynostemma pentaphyllum extract, the hexapeptide and the apple stem cell stock solution in parts by weight at 15 ℃, and freeze-drying to obtain the essence powder.

Comparative example 1

This comparative example is different from example 3 only in that the raw material components do not contain the leukocyte extract, and the other operations are the same as example 3.

Comparative example 2

The comparative example is different from example 3 only in that the rice fermented liquid is not contained in the raw material components, and the other operations are the same as example 3.

Comparative example 3

This comparative example differs from example 3 only in that the raw material ingredients do not contain the flos caryophylli extract, and the other operations are the same as example 3.

Examples of the experiments

Verification of anti-wrinkle effect of the essence powders obtained in the embodiment 3 and the comparative examples 1 to 3

92 subjects with dry skin and water shortage are selected, divided into 4 groups according to age and sex, and 23 persons in each group are respectively an experimental group (corresponding to example 3) and a control group (corresponding to comparative examples 1-3), the subjects use corresponding test essence powder (dissolved by purified water and coated on the face skin) respectively, the test essence powder is used once a day, after one month, VISIA facial image analysis is adopted to measure the skin smoothness of each subject after using each group of essence powder (the test condition is shown in table 1), and the higher the numerical value is, the poorer the skin smoothness is.

TABLE 1 skin smoothness test results

The results show that the skin smoothing effect of the essence powder of the invention in example 3 is more obvious than that of comparative examples 1-3, the skin smoothing effect of the essence powder of the control group does not contain the leukocyte extract, the rice fermentation broth or the flos gynurae formosanae extract, and the skin smoothing effect is obviously reduced, so that the cell extract, the rice fermentation broth and the flos gynurae extract have a synergistic effect and have an anti-wrinkle effect on the skin.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims

1. The anti-wrinkle essence powder is characterized by comprising the following raw material components:

1-2 parts of trehalose;

5-8 parts of rice fermentation liquor;

0.2-0.8 parts by weight of leukocyte extract;

2-4 parts of motherwort extract;

2-4 parts of flos chrysanthemi indici extract;

2-4 parts of gynostemma pentaphylla extract;

0.1-0.3 weight portion of hexapeptide;

0.1-0.3 parts of apple stem cell stock solution.

2. The anti-wrinkle essence powder according to claim 1, wherein the raw material components comprise:

1.5 parts by weight of trehalose;

7.5 parts of rice fermentation liquor;

0.5 weight portion of leucocyte extract;

3 parts of motherwort extract;

3 parts of flos chrysanthemi indici extract;

3 parts of gynostemma pentaphylla extract;

0.2 weight portion of hexapeptide;

0.2 part of apple stem cell stock solution.

3. The anti-wrinkle essence powder according to any one of claims 1 or 2, wherein the leukocyte extract is prepared by a method comprising:

(A1) taking human venous blood, adding an anticoagulant, and slightly and uniformly mixing to obtain diluted blood;

(A2) standing the diluted blood, dividing the diluted blood into three layers, and sucking a middle cell layer to obtain cell sap to be treated;

(A3) adding balanced salt solution into the cell sap to be treated, uniformly mixing, centrifuging, and then discarding supernatant to obtain precipitated cells for amplification, thereby obtaining amplified white blood cells and an amplification culture solution;

(A4) extracting nucleotide from the amplified white blood cells, extracting protein from the amplification culture solution, and mixing the extracted nucleotide and the extracted protein to obtain the white blood cell extract.

4. The anti-wrinkle essence powder according to claim 3, wherein in the step (A1), the anticoagulant is EDTA or Na oxalate, and the concentration of the anticoagulant in blood is 4.45 ± 0.85 μmol;

in the step (A2), the standing temperature is 37 ℃, and the standing time is 50-70 min;

in the step (A3), the balanced salt solution is Ca-free²⁺、Mg²⁺The centrifugation speed is 1500-2500r/min, the centrifugation time is 5-15min, and the amplification uses RPMI1640 culture medium containing 5% FBS and 200U interleukin 2;

in the step (A4), DNAzol is used for extracting the nucleotide in the white blood cell after amplification^TMReagent kit; the method for extracting and amplifying the protein in the culture solution comprises the following steps: and filtering the amplification culture solution through a 0.22 mu m filter membrane to obtain the protein.

5. The anti-wrinkle essence powder according to any one of claims 1 or 2, wherein the rice fermentation liquor is prepared by the following method:

(B1) soaking rice in water, and steaming to obtain cooked rice;

6. The anti-wrinkle essence powder according to any one of claims 1 or 2, wherein the motherwort extract is prepared by the following method:

(C1) drying and crushing motherwort to obtain fine motherwort powder;

7. The anti-wrinkle essence powder according to claim 6, wherein in the step (C1), the particle size of the motherwort fine powder is 50-60 mesh.

8. The anti-wrinkle essence powder according to claim 6, wherein in the step (C2), the mass ratio of the motherwort fine powder to the first addition of distilled water is 1:10-1: 15; the mass ratio of the first filter residue to the second addition of distilled water is 1:10-1: 15;

the concentration is water bath concentration, the concentration temperature is 80-90 ℃, and the aperture of a filter membrane for carrying out the microporous filtration is 0.4-0.5 mu m.

9. The preparation method of the anti-wrinkle essence powder according to any one of claims 1-2, which comprises the following specific steps:

10. The method for preparing the anti-wrinkle essence powder according to claim 9, wherein the mixing temperature is 10-20 ℃.