CN111684994A - Rapid seedling growing method of rhizoma acori graminei - Google Patents
Rapid seedling growing method of rhizoma acori graminei Download PDFInfo
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- CN111684994A CN111684994A CN202010661247.3A CN202010661247A CN111684994A CN 111684994 A CN111684994 A CN 111684994A CN 202010661247 A CN202010661247 A CN 202010661247A CN 111684994 A CN111684994 A CN 111684994A
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- seeds
- ridging
- soaking
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- leaved sweetflag
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a method for quickly culturing grassleaf sweelflag rhizome seedlings, which comprises the following steps: the method comprises the following steps: harvesting the infructescence; step two: soaking seeds; step three: ridging; step four: sowing; step five: coating a film; step six: and (5) cultivating and managing. The invention greatly improves the survival rate of the seedlings, has simple seedling raising process, low seedling raising cost, low technical requirement, strong operability, short seedling raising period and low equipment requirement, and can realize large-scale production.
Description
Technical Field
The invention belongs to the technical field of seedling raising of grass-leaved sweetflag, and particularly relates to a rapid seedling raising method of grass-leaved sweetflag.
Background
Rhizoma Acori Graminei is a perennial herb of Perilla of Labiatae, and is a common Tibetan medicine. The rapid development of biotechnology in recent years has led to the development of the pharmaceutical biotechnology industry. Tissue culture is the most advanced plant propagation technology in the modern, and at present, propagation of the acorus gramineus is mainly carried out in a tissue culture mode.
The tissue culture step of the grass-leaved sweetflag:
1. establishing an aseptic system: selecting full-seed rhizoma Acori Graminei seed, treating with 70% alcohol, performing surface disinfection with 0.1% mercuric chloride, pouring off disinfectant, washing with sterile water for 5-6 times, inoculating into MS culture medium, culturing in light culture room with 12h photoperiod and 1500LX light intensity at 23/14 deg.C, and growing into aseptic seedling for 25-30 d.
2. Propagation culture of aseptic grass-leaved sweetflag seedlings: in order to screen the optimal multiplication culture medium, the grass-leaved sweetflag aseptic seedlings after seed germination are inoculated in MS culture media with different concentrations, and the multiplication times are counted after 30 days.
3. Rooting culture of test-tube plantlets of rhizoma acori graminei: inoculating the aseptic seedling into MS culture medium added with NAA with different concentrations for induction period to root, and observing the rooting condition after 30 d.
The prior art has the following disadvantages:
1. the survival rate of the test tube is low in the prior art;
2. in the prior art, the procedure is complex, the operation difficulty is high, and the operability of mass production is not strong;
3. the prior art is tissue culture, and has higher requirements on equipment and instruments;
4. in the prior art, the seedling raising period is long, and more than 2 months are needed from the establishment of an aseptic seedling system to the final seedling exercising;
5. in the prior art, the cost is high due to high equipment requirement, long seedling culture period, high operation difficulty, complex procedure and low seedling culture survival rate, so that the method is not suitable for large-scale production.
Disclosure of Invention
In order to overcome the problems, the invention provides a rapid seedling growing method of rhizoma acori graminei, which greatly improves the survival rate of seedlings, has simple seedling growing process, low seedling growing cost, low technical requirement, strong operability, short seedling growing period and low equipment requirement, and can realize large-scale production.
The technical scheme adopted by the invention is as follows:
a method for quickly culturing seedlings of rhizoma acori graminei comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking the seeds in 900-1100 times of 75% chlorothalonil diluent for 20-40 minutes, then soaking the seeds in clear water for 12-36 hours, finally washing the seeds once with clear water, and drying the seeds in the shade in a ventilated place;
step three: ridging, selecting sandy loam, opening and ridging, namely, firstly ridging a side ditch by 20 cm-30 cm, then paying off by 1.2m, ridging the ditch by 10cm, cleaning a carriage surface by 1m after ridging, and finely and cleanly treating and flattening the carriage surface;
step four: sowing, namely placing the treated grass-leaved sweetflag seeds in warm water at 35-45 ℃ for soaking for 3-7 minutes, then fishing out and mixing with fine sand to prepare sowing, mixing 10 kg of sand with each 1 kg of grass-leaved sweetflag seeds, uniformly spreading on the ridge surface, spreading 1 kg of seeds per mu of land, uniformly plowing the ridge surface by using a rake, and plowing the ridge surface twice to ensure that the seeds are plowed into the soil;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, herbicide cannot be used, watering is carried out once every 5 to 9 days, and transplanting can be carried out 8 months after germination.
Wherein, the four steps are carried out by warm water soaking: in order to break dormancy, an environmental condition is provided which triggers enzymatic activity in the seed, causing it to break dormancy and begin growing.
Wherein, in the step five, film covering: the method mainly plays roles of heat preservation and moisture preservation, ensures that the seeds can successfully overwinter after germination, and increases the survival rate of the seedlings.
The invention has the following advantages:
1. only nine months are needed before and after the germination acceleration and the final seedling growth, and the seedling culture period is greatly shortened compared with that of the wild seedlings;
2. in the whole seedling raising process, relatively fine instruments are not used basically, the requirement on equipment is relatively low, and the seedling raising device is operable and high in popularization;
3. the used raw materials are silt, organic fertilizer and the like, and the price is relatively low, so the seedling raising cost of the invention is relatively low;
4. in the operation process, complex and fine operation is not involved, and the operability is stronger for ordinary people, so that the practicability and the popularization are very strong;
5. the cultivated seedlings are convenient to transplant and easy to operate.
Detailed Description
The present invention will be further described below, but the present invention is not limited to these.
Example 1
A method for quickly culturing seedlings of rhizoma acori graminei comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking the seeds in 1000 times of a 75% chlorothalonil diluent for 30 minutes, then soaking the seeds in clear water for 24 hours, finally washing the seeds once by the clear water, and drying the seeds in the shade in a ventilated place;
step three: ridging, namely selecting sandy loam, opening boxes and ridging, firstly forming a side ditch of 25cm, then paying off at 1.2m, forming a ridge ditch of 10cm, cleaning a carriage surface by 1m after ridging, and finely, cleanly and flatly treating the carriage surface;
step four: sowing, namely soaking the treated grass-leaved sweetflag seeds in warm water at 40 ℃ for 5 minutes, fishing out the grass-leaved sweetflag seeds, mixing the grass-leaved sweetflag seeds with fine sand for preparing sowing, mixing the grass-leaved sweetflag seeds with sand 10 kg per 1 kg, uniformly spreading the grass-leaved sweetflag seeds on the ridge surface, sowing the grass-leaved sweetflag seeds 1 kg per mu of land, uniformly plowing the ridge surface by using a rake, plowing the ridge surface twice, and plowing the seeds into the;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, herbicide cannot be used, watering is carried out once every 7 days, and transplanting can be carried out 8 months after germination.
Example 2
A method for quickly culturing seedlings of rhizoma acori graminei comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking for 20 minutes by using 900 times of 75% chlorothalonil diluent, then soaking for 12 hours by using clear water, finally washing once by using clear water, and placing in a ventilated place for drying in the shade;
step three: ridging, selecting sandy loam, opening boxes and ridging, firstly ridging a side ditch by 20 cm, then paying off by 1.2m, ridging the ditch by 10cm, cleaning the compartment surface by 1m after ridging, and finely, cleanly and flatly treating the compartment surface;
step four: sowing, namely soaking the treated grass-leaved sweetflag seeds in warm water at 35 ℃ for 3 minutes, fishing out the grass-leaved sweetflag seeds and mixing with fine sand to prepare for sowing, mixing 10 kg of sand with each 1 kg of grass-leaved sweetflag seeds, uniformly spreading the grass-leaved sweetflag seeds on the ridge surface, sowing 1 kg of seeds in each mu of land, uniformly plowing the ridge surface by using a rake, plowing the ridge surface twice, and plowing the seed into the soil;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, herbicide cannot be used, watering is carried out once every 5 days, and transplanting can be carried out 8 months after germination.
Example 3
A method for quickly culturing seedlings of rhizoma acori graminei comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking the seeds in 1100 times of 75% chlorothalonil diluent for 40 minutes, then soaking the seeds in clear water for 36 hours, finally washing the seeds once with clear water, and drying the seeds in the shade in a ventilated place;
step three: ridging, namely selecting sandy loam, opening boxes and ridging, firstly forming a side ditch by 30cm, then paying off by 1.2m, forming a ridge ditch by 10cm, cleaning a carriage surface by 1m after ridging, and finely, cleanly and flatly treating the carriage surface;
step four: sowing, namely soaking the treated grass-leaved sweetflag seeds in warm water at 45 ℃ for 7 minutes, fishing out the grass-leaved sweetflag seeds, mixing the grass-leaved sweetflag seeds with fine sand for preparing sowing, mixing 10 kg of fine sand for every 1 kg of grass-leaved sweetflag seeds, uniformly spreading the grass-leaved sweetflag seeds on the ridge surface, sowing the grass-leaved sweetflag seeds 1 kg of fine sand per mu of land, uniformly plowing the ridge surface by using a rake, plowing the ridge surface twice, and plow;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, herbicide cannot be used, watering is carried out once every 9 days, and transplanting can be carried out 8 months after germination.
Example 4
A method for quickly culturing seedlings of rhizoma acori graminei comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking for 40 minutes by using 900 times of 75% chlorothalonil diluent, then soaking for 12 hours by using clear water, finally washing once by using clear water, and placing in a ventilated place for drying in the shade;
step three: ridging, namely selecting sandy loam, opening boxes and ridging, firstly forming a side ditch by 30cm, then paying off by 1.2m, forming a ridge ditch by 10cm, cleaning a carriage surface by 1m after ridging, and finely, cleanly and flatly treating the carriage surface;
step four: sowing, namely soaking the treated grass-leaved sweetflag seeds in warm water at 35 ℃ for 7 minutes, fishing out the grass-leaved sweetflag seeds and mixing with fine sand to prepare for sowing, mixing 10 kg of sand with each 1 kg of grass-leaved sweetflag seeds, uniformly spreading the grass-leaved sweetflag seeds on the ridge surface, sowing 1 kg of seeds in each mu of land, uniformly plowing the ridge surface by using a rake, plowing the ridge surface twice, and plowing the seeds into the soil;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, herbicide cannot be used, watering is carried out once every 5 days, and transplanting can be carried out 8 months after germination.
Example 5
A method for quickly culturing seedlings of rhizoma acori graminei comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking for 20 minutes by using 1100 times of 75% chlorothalonil diluent, soaking for 36 hours by using clear water, finally washing once by using clear water, and drying in the shade in a ventilated place;
step three: ridging, selecting sandy loam, opening boxes and ridging, firstly ridging a side ditch by 20 cm, then paying off by 1.2m, ridging the ditch by 10cm, cleaning the compartment surface by 1m after ridging, and finely, cleanly and flatly treating the compartment surface;
step four: sowing, namely soaking the treated grass-leaved sweetflag seeds in warm water at 45 ℃ for 3 minutes, fishing out the grass-leaved sweetflag seeds, mixing the grass-leaved sweetflag seeds with fine sand for preparing sowing, mixing the grass-leaved sweetflag seeds with sand 10 kg per 1 kg, uniformly spreading the grass-leaved sweetflag seeds on the ridge surface, sowing the grass-leaved sweetflag seeds 1 kg per mu of land, uniformly plowing the ridge surface by using a rake, plowing the ridge surface twice, and plowing the seeds into the;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, herbicide cannot be used, watering is carried out once every 9 days, and transplanting can be carried out 8 months after germination.
Wherein, the four steps are carried out by warm water soaking: in order to break dormancy, an environmental condition is provided which triggers enzymatic activity in the seed, causing it to break dormancy and begin growing.
Wherein, in the step five, film covering: the method mainly plays roles of heat preservation and moisture preservation, ensures that the seeds can successfully overwinter after germination, and increases the survival rate of the seedlings.
Example 1 in the present application is the best mode.
It is noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (5)
1. A method for quickly culturing seedlings of grassleaf sweelflag rhizome is characterized in that: the method comprises the following steps:
the method comprises the following steps: harvesting infructescence, harvesting mature infructescence 7 months per year, selecting full rhizoma Acori Graminei seeds, cleaning with clear water, and threshing;
step two: soaking seeds, soaking in chlorothalonil diluent, soaking in clear water, washing with clear water, and drying in the shade;
step three: ridging, namely selecting sandy loam, ridging, firstly ridging a side ditch, then paying off at 1.2m, ridging the ditch, cleaning the carriage surface after ridging, and finely, cleanly and flatly treating the carriage surface;
step four: sowing, namely soaking the treated grass-leaved sweetflag seeds in warm water, fishing out the grass-leaved sweetflag seeds and mixing with fine sand to prepare sowing, mixing 10 kg of sand with 1 kg of grass-leaved sweetflag seeds, uniformly spreading the mixture on the ridge surface, spreading 1 kg of seeds in each mu of land, uniformly plowing the ridge surface by using a rake, and plowing the ridge surface twice to ensure that the seeds are plowed into the soil;
step five: covering a film, uniformly watering the compartment surface once, then covering the film, compacting the periphery by using soil blocks, and compacting the protruding part of the compartment surface at intervals of 2m by using the soil blocks;
step six: intertillage management, after the seeds germinate, white films are removed, manual intertillage weeding is carried out, no herbicide is used, watering is carried out regularly, and transplanting can be carried out 8 months after germination.
2. The method for rapidly growing seedlings of acorus gramineus according to claim 1, which is characterized in that: the chlorothalonil obtained in the second step is 75% of chlorothalonil, the dilution multiple is 900-1100 times, the soaking time of the chlorothalonil is 20-40 minutes, and the soaking time of the chlorothalonil in clear water is 12-36 hours.
3. The method for rapidly growing seedlings of acorus gramineus according to claim 1, which is characterized in that: in the step three, the side ditch is 20 cm-30 cm, the ridge ditch is 10cm, and the clean compartment surface after ridge forming is 1 m.
4. The method for rapidly growing seedlings of acorus gramineus according to claim 1, which is characterized in that: the temperature of the warm water in the fourth step is 35-45 ℃, and the soaking time is 3-7 minutes.
5. The method for rapidly growing seedlings of acorus gramineus according to claim 1, which is characterized in that: and watering once every 5 to 9 days in the sixth step.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112219662A (en) * | 2020-10-14 | 2021-01-15 | 重庆市药物种植研究所 | Propagation method of rhizoma acori graminei |
| CN112243803A (en) * | 2020-10-16 | 2021-01-22 | 湖南湘枳生物科技有限责任公司 | Greenhouse seedling raising method for acorus gramineus seeds |
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| CN104170615A (en) * | 2014-07-18 | 2014-12-03 | 宁国千方中药发展有限公司 | Acorus gramineus planting method |
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2020
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112219662A (en) * | 2020-10-14 | 2021-01-15 | 重庆市药物种植研究所 | Propagation method of rhizoma acori graminei |
| CN112243803A (en) * | 2020-10-16 | 2021-01-22 | 湖南湘枳生物科技有限责任公司 | Greenhouse seedling raising method for acorus gramineus seeds |
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Application publication date: 20200922 |
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