CN111683740B - 带电的纳米气泡分散液、其制造方法及制造装置,以及使用该纳米气泡分散液控制微生物及植物生长速度的方法 - Google Patents
带电的纳米气泡分散液、其制造方法及制造装置,以及使用该纳米气泡分散液控制微生物及植物生长速度的方法 Download PDFInfo
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Abstract
本发明的带电的纳米气泡分散液,旨在提供带正电或负电的纳米气泡,明确带正电或负电的纳米气泡对微生物和植物的生长的影响。提供一种纳米气泡分散液,含有105‑1010个/cc的微小气泡,所述微小气泡分散在液体中,带正电或负电,平均粒径是10‑500nm,Zeta电位是10‑200mV。
Description
技术领域
本发明涉及一种带电的纳米气泡分散液、其制造方法及制造装置,以及使用该纳米气泡分散液控制微生物及植物生长速度的方法,更具体而言,涉及一种具有带电性的纳米气泡分散液、容易且廉价地制造该纳米气泡分散液的制造方法及制造装置,以及使用该纳米气泡分散液控制微生物及植物生长速度的方法。
背景技术
以往,含有直径不到1微米的气泡(所谓纳米气泡)的液体,其气泡的浮力相对较小导致气泡会长时间留在液体中以及带负电等性质受到关注,被应用在硅晶片清洗、养殖业的效率化等领域。
再有,对于纳米气泡的产生,提出了如专利文献1中所示的在水中产生的方法。
进而再有,使用纳米气泡给生物的生理活性带来改变的技术受到关注。例如在专利文献2中,记载了可用于促进植物生长等目的的内容。
现有技术文献
专利文献
专利文献1:日本专利第4144669号公报
专利文献2:日本专利公开2009-131769号公报
发明内容
发明要解决的技术问题
在以往的纳米气泡技术中,由于(1)与纳米气泡的制造相关的价格和能源成本太高,(2)无法产生带正电的纳米气泡,(3)对生物的影响不明确,导致经济性以及可实施技术的领域都有限。
(1)对于与纳米气泡的制造相关的成本
以往,提出过使风扇在气液多相流中高速旋转,向气液多相流中施加高压,导入漏斗状的管内,产生空化的方法等,但装置结构复杂,每台装置的价格高,因此可实施纳米气泡技术的领域受到限制。
再有,由于在液相中产生了纳米气泡,所以液体导致可动部受到很大的抵抗,浪费很多能源,从经济性观点看可实施技术的领域受到限制。
(2)对于带正电的纳米气泡
以往,只能产生带负电的纳米气泡,由于这种纳米气泡只能与带正电的离子和物质表面结合,所以可实施技术的领域受到限制。
(3)对于带给生物的影响
以往,只能产生带负电的纳米气泡,纳米气泡带给生物的影响究竟是封入纳米气泡内气体的影响导致的,还是纳米气泡带的负电导致的,再或者是纳米气泡的物理碰撞导致的,难以得出结论。
为了解决这样的技术问题,本发明的第一技术问题是提供带正电和带负电的纳米气泡。
再有,本发明的第二技术问题是明确带正电和带负电的纳米气泡对微生物及植物的生长的影响。
进而,本发明的技术问题是提供一种在偏远地区和山区的农业设施中也能实施纳米气泡技术的、可运输、可在使用现场制造纳米气泡的纳米气泡制造装置。
解决技术问题的手段
用于解决这样的技术问题的本发明的带电纳米气泡分散液,其特征在于:含有105-1010个/cc的微小气泡,所述微小气泡分散在液体中并带正电或负电,平均粒径是10-500nm,Zeta电位是10-200mV。
再有,所述带电的纳米气泡分散液优选带正电。
再有,本发明的带电的纳米气泡分散液的制造方法,其特征在于:在成为气泡的气体气氛中,将微小化到微米大小的液体进一步粉碎,从而产生被所述液体围绕并带电的纳米气泡,使用重力、离心力,电磁力等来采集。
再有,可通过给所述气体气氛施加电场,并将负侧接地,使得产生带负电的纳米气泡,通过将粉碎的材料接地,使得产生带正电的纳米气泡。
再有,本发明的带电的纳米气泡分散液的制造装置,其特征在于:通过权利要求3或4记载的制造方法制造权利要求1记载的纳米气泡分散液。
再有,使用本发明的带电的纳米气泡分散液的制造方法,可与阳离子性物质或阴离子性物质结合或解离(乖離)。
再有,使用本发明的带电的纳米气泡分散液的制造方法,可制造依赖于纳米气泡带电性的氧化剂或还原剂。
再有,使用本发明的带电的纳米气泡分散液,可促进或抑制微生物的增殖,以及促进或抑制植物的生长。
发明效果
以往,由于在液相中产生纳米气泡,因此液体导致可动部受到大的抵抗,消耗很多能源,从经济合理性观点出发,可实施技术的领域受到限制。
但是,在本发明中,可将产生纳米气泡的机械置于气相内,减少了能源的消耗量,从而可实施技术的领域大幅扩展。
本发明的纳米气泡制造装置,可用简单的结构产生纳米气泡,因此可实施技术的领域大幅扩展。再有,由于尺寸变为可用小型车辆运送的程度,因此在使用现场可容易又便宜地制造纳米气泡,从而偏远地区和山区的农业设施中也能实施纳米气泡技术。
在本发明中,通过改变材料和液体的组合,可分别制造带正电的纳米气泡和带负电的纳米气泡,根据用途可提供正的纳米气泡或负的纳米气泡。
再有,通过可分别制造带正电和带负电的纳米气泡,很明显纳米气泡具有可对物质施与或接受电子的性质。
通过纳米气泡具有可对物质施与或接受电子的性质,可只通过水和空气制造带有所需的氧化力或还原力、在规定时间后分解的氧化剂和还原剂。由此,对于如果大量使用氧化剂或还原剂的话,虽然理论上可从土壤中渗出而去除,但是之后会产生严重的二次污染,因此难以实施的土壤中的盐分去除和放射性物质的去除等,也可通过纳米气泡技术来实施。
进而,由于本发明可产生带正电的纳米气泡,因此对于纳米气泡具有的带电性带给生物的影响,可进行比较对照实验。结果,利用带正电或负电的纳米气泡,可促进或抑制微生物和植物的增殖、生长等。通过将本发明中所示的纳米气泡例如导入到自来水或培养液等中,使微生物或植物的根或叶吸收,可对生长起到促进或抑制效果。
另一方面,在微生物中,既有医学药品的制造、生物燃料的制造、酿造等对社会有用的微生物,也存在病原菌等有害的微生物,而可按适当的时机来对这些微生物进行抑制、增殖。
附图说明
图1是本发明的实施例1的带正电的纳米气泡的Zeta视图。
图2是本发明的实施例1的带负电的纳米气泡的Zeta视图。
图3是对衣藻使用封入空气的纳米气泡使其进行光合成时测量所得到的叶绿素产量的图表。
图4是对衣藻使用封入二氧化碳气体的纳米气泡使其进行光合成时测量所得到的叶绿素产量的图表。
图5是对衣藻使用封入空气的纳米气泡设置12小时的黑暗期测量叶绿素产量时测量所得到的叶绿素产量的图表。
图6是在小松菜LED光合成栽培模型中,带正电和负电的纳米气泡的生长促进效果的比较图。
图7是示出在番茄的温室栽培模型中带正电的纳米气泡的效果的图表。
具体实施方式
下面参照附图详细记载本发明的实施方式。该记载用于说明本发明,并不是通过该记载限定本发明的技术范围。可在不脱离本发明的技术范围的范围内进行多种变更并实施。
本发明的带正电或负电的纳米气泡的平均粒径优选是10~500nm,更优选是50~300nm。平均粒径超过500nm则气泡的浮力大,气泡彼此容易相遇,存在分散液不稳定的情况,平均粒径不到10nm的气泡使用本发明的方法制作是困难的。
再有,本发明的带正电或负电的纳米气泡的Zeta电位优选是10~200mV或-10~-200mV,优选是50~150mV或-50~-150mV。10mV~-10mV(但不包括-10mV)的纳米气泡存在带电效果不足的情况,而-200mV以下或超过200mV并带电是困难的。
进而,本发明的纳米气泡分散液中含有的带电气泡的个数优选是105~1010个/cc,更优选是105~109个/cc。纳米气泡分散液中含有的带电气泡的个数不到105个/cc,则存在带电效果不足的情况,而制造超过109个/cc的纳米气泡分散液是困难的。
本发明的带电的纳米气泡优选带正电。带负电的纳米气泡也显示出比不带电的纳米气泡优越的性质,但带正电的纳米气泡比带负电的纳米气泡具有更多优越性质。
(纳米气泡分散液的制造)
以下,使用的纳米气泡在成为气泡的气体气氛中使微小化到微米大小的液体进一步粉碎,从而产生被所述液体围绕的带电的纳米气泡,对其使用重力、离心力、电磁力等进行收集,从而可制造所述液体带电的纳米气泡分散液。
可通过给气体气氛施加电场并将负侧接地,来产生带负电的纳米气泡,通过将粉碎的材料接地,产生带正电的纳米气泡。
图1中示出本发明的实施例1的正的纳米气泡的Zeta电位和具有该Zeta电位的气泡的频率(Zeta视图),图2中示出实施例2的带负电的纳米气泡的Zeta视图。
(带电的纳米气泡的效果)
本发明的带电的纳米气泡提供一种方法,其制造与阳离子性物质或阴离子性物质结合或解离的物质。
再有,本发明的带电的纳米气泡,通过纳米气泡具有可施与或接受电子的性质,可只通过水和空气制造带有所需的氧化力或还原力、在规定时间后分解的氧化剂和还原剂。
进而,本发明的带电的纳米气泡由于可产生带正电的纳米气泡,因此对于纳米气泡具有的带电性带给生物的影响,可进行比较对照实验。结果,利用带正电或负电的纳米气泡,可促进或抑制微生物和植物的增殖、生长等。通过将本发明中所示的纳米气泡例如导入到自来水或培养液等中,使微生物或植物的根或叶吸收,可对生长起到促进或抑制效果。
[实施例1]带正电的纳米气泡的制造
向密闭的空气气氛中提供微小化到微米大小的水,多个旋转体设置为相邻设置的旋转体彼此朝相反方向旋转,使用该多个旋转体,将微小化到微米大小的水进一步粉碎,收集所生成的雾体,从而可得到被水围绕并带正电的纳米气泡。所得到的纳米气泡的按直径密度和按电荷密度,使用麦奇克拜尔公司(マイクロトラックベル社)的纳米气泡电荷测量,以Zeta视图(ZetaView)+T.大平带电盘(T.Ohdaira荷電ディスク)法计算并测量,气泡的平均粒径设置为兵库县的spring9,使用具有欧米伽伦茨(Omega Lenz)的超高电压电子显微镜(Ultra-high voltage electron microscope)来测量。
带正电的纳米气泡的按电荷密度(Zeta视图)在图1中示出。
[实施例2]充为负电的纳米气泡的制造
向密闭的空气气氛中施加高电压并使负侧接地,提供微小化到微米大小的水,多个旋转体设置为相邻的旋转体朝相反方向旋转,使用该多个旋转体,将微小化到微米大小的水进一步粉碎,收集所生成的雾体,从而可得到被水围绕并带负电的纳米气泡。图2示出带电的纳米气泡的按电荷密度。
(使用纳米气泡分散液控制微生物和植物的生长速度的方法)
[实施例3]使用封入空气的纳米气泡的情况
使用野生型衣藻(NIES-2235株Chlamydomonas reinhardtii,以下仅称为“衣藻”。),分成含有封入空气并带正电的纳米气泡的培养液(正群)、含有带负电的纳米气泡的培养液(负群)、不含有纳米气泡的培养液(对照群)三类,测量各群的叶绿素产量。
·培养株:衣藻
微生物学名(NIES株编号):NIES-2235株
培养基:C培养基(琼脂和瓶子也一样为C培养基)
购买来源:国立研究开发法人国立环境研究所微生物系统保存设施
·对衣藻,使用平型培养皿,从距离25cm的上面向培养皿内的微生物连续照射最适合光合成的、具有620纳米-630纳米的波长峰值的光。培养基使用HSM琼脂培养基。
·纳米气泡样本
正群,以实施例1中记载的方法,使用在碳酸气体中带正电的纳米气泡制造培养基。
负群,以实施例2中记载的方法,使用在碳酸气体中带负电的纳米气泡制造培养基。
对照群,使用不含纳米气泡的蒸馏水,制作培养基。
·对所培养的衣藻,每隔规定时间就使用丙酮的叶绿素提取法进行提取,使用分光光度计(纳米德普(NanoDrop)公司制造:ND-1000)测量所得到的叶绿素。
·测量结果在图3中示出。
如图3所示,与对照群相比,正群在诱导期和对数增殖期中增殖速度显著增加。与对照群相比,负群的增殖速度下降。
[实施例4]使用封入二氧化碳气体的纳米气泡的情况
与实施例3相同,但在二氧化碳气体中制造纳米气泡样本的正群和负群,测量各群的叶绿素产量。
测量结果在图4中示出。与实施例1相同,正群比对照群增殖快,负群比对照群增殖慢。
[实施例5]使用封入空气的纳米气泡,设置12小时的黑暗期的情况
与实施例3相同,但设置12小时的黑暗期,测量叶绿素产量。
测量结果在图5中示出。
如图5所示,正群比对照群增殖快,但负群和对照群的增殖速度相同。
[实施例6]小松菜LED光合成栽培模型
使用小松菜比较水培中带电纳米气泡水的效果。
栽培条件
温度LED灭灯时20℃,LED亮灯时27℃
液体肥料海珀奈克斯特(Hyponext)
纳米气泡水按实施例1、2制作。
气泡平均粒子直径180nm(100~200nm)
气泡密度3.0×108(个/cc)
纳米气泡电荷测量横式麦奇克拜尔公司
计算方法Zeta视图+T.大平带电盘法
播种后第28日的照片在图6中示出。
[实施例7]萝卜光合成模型的生长差
使用萝卜比较水培中带电纳米气泡水的效果。结果在表1中示出。
如表1所示,使用正纳米气泡时,与对照组相比显示1.7~2.2倍的生长速度,使用负纳米气泡时,与对照组相比显示1.1~1.2倍的生长速度。
[表1]
对照组 | 正纳米气泡 | 负纳米气泡 | |
须根长度 | 100 | 222 | 108 |
营养根的体积 | 100 | 171 | 119 |
叶的面积 | 100 | 165 | 117 |
[实施例8]番茄的温室栽培模型中的收获量
图7示出从2017年4月开始导入带正电的纳米气泡产生器的番茄温室栽培模型中的从2016年12月16日-2017年6月16的累积收获量。
如图7所示,导入带正电的纳米气泡产生器的从2017年4月开始的收获量显著增加,截止到2017年4月为止和对照组收获量相同,但在截止到2017年6月16日为止的两个月时间里增收了11%。
Claims (7)
1.一种带正电的纳米气泡分散液的制造方法,其特征在于:
在密闭了含有气体的液体的气体气氛中,所述气体是成为气泡的气体,通过旋转体使微小化到微米大小的含有所述气体的所述液体进一步粉碎,使其带电,
生成通过将所述旋转体接地而带正电的雾体,并且是被所述液体所围绕并带电且被纳米气泡化的含有所述气体的雾体,
适当采集生成的带正电的雾体,从而制造带正电的纳米气泡分散液。
2.根据权利要求1记载的带正电的纳米气泡分散液的制造方法,其特征在于:所述纳米气泡分散液是带正电的纳米气泡分散液,
含有105-1010个/cc的带正电的微气泡,所述微气泡分散在液体中,平均粒径是10-500nm,Zeta电位是+10~+200mV。
3.根据权利要求2记载的带正电的纳米气泡分散液的制造方法,在所述带正电的纳米气泡分散液中,其特征在于:使野生型衣藻的增殖速度增加。
4.根据权利要求2记载的带正电的纳米气泡分散液的制造方法,在所述带正电的纳米气泡分散液中,其特征在于:促进小松菜LED光合成栽培模型的水培的小松菜和促进萝卜光合成模型的水培的萝卜的生长。
5.根据权利要求2记载的带正电的纳米气泡分散液的制造方法,在所述带正电的纳米气泡分散液中,其特征在于:可用于增加温室栽培的番茄的收获量。
6.根据权利要求2记载的带正电的纳米气泡分散液的制造方法,在所述带正电的纳米气泡分散液中,其特征在于:具有依赖纳米气泡的带正电性的氧化力,作为在一定时间后会分解的氧化剂而使用。
7.一种带正电的纳米气泡分散液的制造装置,其特征在于:具备:密封了含有成为气泡的气体的液体的气体气氛;以及使液体进一步粉碎的旋转体;
在所述气体气氛中,将微小化到微米大小的含有所述气体的液体使用所述旋转体进一步粉碎,使其带电,通过将所述旋转体接地,生成带正电的含有所述气体的纳米气泡的雾体,适当采集生成的雾体。
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US4144669A (en) | 1977-06-13 | 1979-03-20 | Takara Co., Ltd. | Multiple function water-going toy |
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CA2547024C (en) | 2003-12-22 | 2013-12-17 | Bracco Research Sa | Gas-filled microvesicle assembly for contrast imaging |
JP4144669B2 (ja) * | 2004-03-05 | 2008-09-03 | 独立行政法人産業技術総合研究所 | ナノバブルの製造方法 |
JP4430609B2 (ja) * | 2005-11-14 | 2010-03-10 | ヤーマン株式会社 | ナノバブル発生装置 |
JP4921333B2 (ja) | 2007-11-29 | 2012-04-25 | 株式会社Reo研究所 | 二酸化炭素ナノバブル水の製造方法 |
JP4921332B2 (ja) * | 2007-11-29 | 2012-04-25 | 株式会社Reo研究所 | 窒素ナノバブル水の製造方法 |
JP5053115B2 (ja) * | 2008-02-05 | 2012-10-17 | 芝浦メカトロニクス株式会社 | 基板の処理装置及び処理方法 |
JP5209357B2 (ja) | 2008-03-28 | 2013-06-12 | 芝浦メカトロニクス株式会社 | 処理液の製造装置、製造方法及び基板の処理装置、処理方法 |
KR101001484B1 (ko) | 2008-06-19 | 2010-12-14 | 주식회사 동부하이텍 | 화학 기상 증착 설비내 머플존의 파우더 제거장치 |
JP4870174B2 (ja) | 2009-01-19 | 2012-02-08 | シャープ株式会社 | 水処理装置および水処理方法 |
TWI551343B (zh) * | 2009-08-06 | 2016-10-01 | Ligaric Co Ltd | Composition and method for producing the same |
JP2011152513A (ja) * | 2010-01-27 | 2011-08-11 | Panasonic Electric Works Co Ltd | 気液混合液生成装置 |
JP2012108073A (ja) | 2010-11-19 | 2012-06-07 | Toshiba Corp | 除染方法および除染装置 |
KR101330863B1 (ko) * | 2012-07-09 | 2013-11-18 | 박정우 | 나노 기포 발생기 |
WO2014050910A1 (ja) | 2012-09-26 | 2014-04-03 | 武田薬品工業株式会社 | 固体粒子の製造方法 |
JP2015097509A (ja) | 2013-11-19 | 2015-05-28 | サンスター技研株式会社 | 超微細粒子を利用した植物栽培方法 |
JP6394201B2 (ja) | 2014-09-03 | 2018-09-26 | サンスター株式会社 | 希釈農薬の調整方法及び希釈農薬 |
JP6522969B2 (ja) | 2015-01-30 | 2019-05-29 | 三菱重工業株式会社 | 放射性物質の除去方法 |
CN105417674A (zh) * | 2015-11-23 | 2016-03-23 | 天津颐品农庄电子商务有限公司 | 一种微纳米气泡水的制备方法和应用 |
CN109415686B (zh) * | 2016-05-13 | 2023-02-21 | 株式会社希古玛科技 | 可给药到活体的水溶液及其制备方法 |
CN105855065B (zh) * | 2016-06-20 | 2018-03-09 | 中国矿业大学 | 一种基于纳米气泡矿浆预处理的氧化煤泥分选方法 |
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2017
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JP2023001157A (ja) | 2023-01-04 |
KR20200093044A (ko) | 2020-08-04 |
EP3721979A1 (en) | 2020-10-14 |
US20210244021A1 (en) | 2021-08-12 |
EP3721979A4 (en) | 2022-01-12 |
JP7227694B2 (ja) | 2023-02-22 |
CN111683740A (zh) | 2020-09-18 |
KR20240006001A (ko) | 2024-01-12 |
JP2019103958A (ja) | 2019-06-27 |
KR102657332B1 (ko) | 2024-04-12 |
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