CN111657145A - Method for rapidly cultivating dysosma versipellis seedlings - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention belongs to the technical field of planting of traditional Chinese medicinal materials, and relates to a method for quickly cultivating dysosma versipellis seedlings, which comprises the following specific steps: washing fresh dysosma versipellis with a large number of fibrous roots with water, soaking in an inducing solution, taking out the dysosma versipellis with the roots, placing on silt, and repeating for 5-6 times after illumination for 2-3 hours; cultivating the seeds in sand in the dark for 2-3 days; then cutting the Dysosma versipellis roots into small sections; soaking the substrate in the treatment solution, taking out the substrate, directly inserting the substrate into a nutrient medium with the depth of 1.5-2.5 cm, and culturing for 15-30 days by illumination and standing; spraying nutrient solution every 1d when a bud emerges until one leaf grows out completely; and regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 50-70% and the environmental temperature at 20-25 ℃ until the seedlings are poured, and taking the seedlings with bud heads to transplant into the field.
Description
Technical Field
The invention belongs to the technical field of planting of traditional Chinese medicinal materials, and relates to a method for quickly cultivating dysosma versipellis seedlings.
Background
Dysosma versipellis is a perennial herb of the genus Podophyllum of the family berberidaceae, and the plant height can reach 150 cm. The rhizomes grow transversely and roughly and have multiple fibrous roots; the cauline leaves are thin paper, nearly round, wide and triangular in split pieces, oval or oval-shaped and long round, hairless on the upper surface, obviously raised leaf veins and thin teeth on the edges; the flower stalk is fine, the flower is deep red, sepals are long and elliptical, and petals are spoon-shaped and inverted egg-shaped and have no hair; the filament is shorter than anther, ovary is oval, berry is oval, and most seeds are. Flowering in 3-6 months and bearing fruit in 5-9 months. Is distributed in Hunan, Hubei, Zhejiang, Jiangxi, Anhui, Guangdong, Guangxi, Yunnan, Guizhou, Sichuan, Henan and Shaanxi in China. Living under mountain slopes, shrubs, stream-side wet and shady places, bamboo forests or lime evergreen forests. The altitude is 300-2400 m. The seed is a common Chinese herbal medicine for folks, can dispel wind and eliminate phlegm, disinfect and relieve swelling, kill parasites when used as a medicine, has special detoxifying effects, can treat snake bites, and is a national secondary protection plant.
Chinese patent publication No. CN 1586168A discloses a tissue culture rapid propagation method of dysosma versipellis, which comprises 1) selection and treatment of explants: selecting buds of dysosma versipellis as culture materials, washing with liquid detergent and tap water, washing under running water for 10-30 minutes, treating in 75% alcohol for 10-30 seconds, sterilizing with 0.1-0.15% mercuric chloride for 1-10 minutes, washing with sterile water, cutting off the discolored part of the explant, and inoculating in a culture medium; 2) and (3) induction culture: using MS + BA (0.2-0.5mg/l) + ZT (0-1.0mg/l) as an induction culture medium, and inoculating the selected bud in the culture medium; 3) and (3) proliferation culture: transplanting explants growing on an induction culture medium onto MS + BA (0.2-1.0mg/l) + ZT (0-0.5mg/l) + GA (0-0.2mg/l) + PVP (0-0.5mg/l) for continuous culture; 4) rooting culture: selecting 1/2MS + BA (0.2-0.5mg/L) + ZT (0.1-0.5mg/L) as rooting medium; 5) transplanting: taking out the rooted plants, and transplanting the plants onto a substrate mixed by perlite and peat soil (1: 1-1.5).
Chinese patent publication No. CN105104199A discloses a method for producing artificial seeds of dysosma versipellis, comprising: differentiating and culturing dysosma versipellis embryonic callus to obtain a somatic embryo, then carrying out proliferation culture on the somatic embryo to obtain a proliferated somatic embryo, wrapping the proliferated somatic embryo with an embedding medium to obtain the dysosma versipellis artificial seed, and finally spraying 2.5-40g/L pullulan solution on the dysosma versipellis artificial seed for storage to improve the germination rate of the artificial seed.
Chinese patent publication No. CN 108849209a discloses an artificial cultivation method for interplanting polygala fallax hemsl and dysosma versipellis, which comprises the following steps: (1) selecting land: selecting a hilly land with sufficient water source and weak acidity soil, wherein the slope is 15-20 ℃; (2) seedling culture: seedlings of the polygala fallax hemsl are cultivated in advance by adopting a cutting seedling method, and seedlings of the dysosma versipellis are cultivated by adopting a rhizome propagation method; (3) transplanting the polygala fallax hemsl: transplanting the polygala fallax hemsl seedlings after 3 months of spring rain, and transplanting after properly irrigating water if no rain exists, wherein the soil surface is moist and does not cake; preparing soil according to a conventional technology, digging pits, planting at a row spacing of 40-55 cm, a plant spacing of 80-100 cm, a depth of 10-20 cm and a pit length of 20cm, transplanting cutting seedlings on one side of the pits, applying a proper amount of nitrogen-phosphorus-potassium compound fertilizer as a base fertilizer on the other side of the pits, applying an average amount of 15-20 g of each plant of the base fertilizer, covering soil, tamping the soil surface, and pouring a proper amount of root fixing water; after transplanting for 10-15 days, checking seedlings, and timely replenishing seedlings when dead seedlings are found; (4) transplanting dysosma versipellis: after the radix Polygalae fallax is transplanted, digging planting holes in gaps between rows of the radix Polygalae fallax, planting two rows of dysosma versipellis in the middle of every two parallel radix Polygalae fallax, wherein the distance between the two rows is 20cm, and the plant spacing is 20-30 cm, transplanting roots and stems into the planting holes with soil, covering soil, and pouring a proper amount of root fixing water; after transplanting is finished, covering a sun-shading film on a planting area to serve as a protective film, preventing seedlings from being sunburned by strong light, and removing the film when the seedlings are strong after 8-9 months of early autumn; (5) field management: A. fertilizing: intertillage weeding is carried out on the falia fallax hemsl in 6 months after transplanting, a proper amount of farmyard manure is applied, the application amount of each farmyard manure is 30-40 g, and the farmyard manure is applied once again after the Dysosma versipellis is planted in 9 months, wherein the application amount of each farmyard manure is 40-50 g; fertilizing respectively in 3, 6 and 9 months in the second year, and applying for three times in total, wherein the fertilizing amount is 50-70 g per plant each time; applying farmyard manure for 2-3 times per year on the dysosma versipellis, preferably synchronously applying fertilizer for 20-30 g per plant on average; a fertilizing mode: digging holes in the original base fertilizer application position by adopting a hole application mode, wherein the depth is 10-20 cm, and covering soil after fertilization; carrying out furrow application on the dysosma versipellis, digging a furrow with the depth of 5-10 cm at a position 10cm away from one side of the dysosma versipellis, covering soil after applying farmyard manure, and slightly tamping; B. watering: irrigating and watering according to specific drought conditions, wherein watering is carried out once in 4-8 days generally in rainy days in summer, watering is carried out once in 15-20 days in winter, and the watering amount can be properly reduced by the polygala fallax hemsl; C. weeding: weeding for 2-3 times every year at regular intervals, preferably weeding before fertilization, and preventing weeds from robbing fertilizer nutrition; the weeds are burnt after being dried in the sun, and are added into farmyard manure as plant ash; D. and (3) pest control: spraying after diluting 200-250 times by using 40% carbendazim suspending agent under the condition of insect damage; (6) harvesting: picking the leaves of the polygala fallax hemsl in the current year, and harvesting the whole plant after the seeds are mature in 10 months in the third year; the rootstock and root of Dysosma versipellis are used as medicine, transplanted to overground part in 10 months of the third year, harvested, dug, removed stem and leaf, cut the first and second nodes with bud heads as seeds, and the rest are cleaned, dried in the sun or dried to be used as medicinal materials.
The above literature documents mention that the conventional breeding mode of the division of the Dysosma versipellis roots and the seed cultivation has low breeding rate and high production cost, and is not beneficial to the protection of wild resources. Although the problem of rapid propagation of dysosma versipellis can be solved to a certain extent by utilizing a tissue culture rapid propagation mode, the bottleneck problems of variation and germplasm degradation of callus after subculture for multiple generations, low transplanting survival rate of callus differentiated seedlings, high production cost and the like exist at the same time, and the large-scale cultivation production of dysosma versipellis is limited to a great extent.
Disclosure of Invention
The invention aims to solve the problems of dysosma versipellis at present, provides a method for quickly cultivating dysosma versipellis seedlings, and aims to utilize a large number of fibrous roots of dysosma versipellis to quickly breed the dysosma versipellis seedlings so as to reduce the production cost of drug farmers and the difficulty of breeding technologies.
Therefore, the invention provides a method for quickly cultivating dysosma versipellis seedlings, which comprises the following specific steps:
(1) washing fresh dysosma versipellis (whole plant) with a large number of fibrous roots with water for two to three times, soaking in an inducing solution for 5-10 min, taking out the dysosma versipellis with roots, directly placing the dysosma versipellis with roots on silt, after illuminating for 2-3 h under the condition that the illumination intensity is 300-;
(2) directly cultivating the Dysosma versipellis with roots treated in the step (1) in the sand in the dark, keeping the air humidity at 70-85%, and the environmental temperature at 20-25 ℃, and cultivating for 2-3 d;
(3) taking the Dysosma versipellis with roots treated in the step (2) out of the sediment, and cutting the Dysosma versipellis fibrous roots into small segments of fibrous roots with lengths of 4-6 cm by using scissors;
(4) soaking the obtained small segments of fibrous roots in a treatment solution for 3-10 min, taking out, directly inserting the small segments of fibrous roots into a nutrient substrate, keeping the depth at 1.5-2.5 cm, placing and culturing under the condition that the illumination intensity is 300 and 500lx, keeping the air humidity at 70-85%, keeping the environmental temperature at 20-25 ℃, and culturing for 15-30 d, wherein the formula of the treatment solution is 3-8 mg/LNAA + 6-10% of carbendazim aqueous solution;
(5) when the bud heads emerge from the fibrous roots of the small segments, spraying 1-2.5 kg/m of the fertilizer at intervals of 1 day2The nutrient solution is prepared from 1-3 g/L ammonium nitrate, 5-10 g/L potassium nitrate, 0.01-0.1 g/L glycine, 0.1-0.4 g/L inositol, 0.1-0.6 mg/LNAA and 2-4% carbendazim aqueous solution until one leaf grows out completely.
(6) And regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 50-70% and the environmental temperature at 20-25 ℃ until the seedlings are poured, and taking the seedlings with bud heads to transplant into the field.
The above nutritional matrix is preferably water-retaining matrix such as moss matrix or coconut husk matrix.
The foliar fertilizer can be directly purchased from the market, and can also be replaced by 0.5-2 g/L monopotassium phosphate.
Technical effects
1. The seedling is rapidly bred by adopting the Dysosma versipellis fibrous root, the breeding speed of the Dysosma versipellis is greatly improved, the root system is developed, the genetic stability of the Dysosma versipellis can be ensured to a great extent, and the variation probability is reduced.
2. The method disclosed by the invention is simple to operate, makes full use of the Dysosma versipellis fibrous roots, greatly reduces the production cost of Dysosma versipellis seedling culture, simplifies the production process, and reduces the technical difficulty of large-scale Dysosma versipellis seedling culture production.
Detailed Description
+0.3g/L the following examples of the invention are intended to illustrate the essence of the invention but are not intended to limit the invention thereto.
Example 1
Rinsing fresh radix et rhizoma Dysosmatis with large amount of fibrous root with water for three times, and placingSoaking in an aqueous solution containing 2mg/L6-BA (6-benzylaminopurine) +4mg/LNAA (naphthylacetic acid) + 6% carbendazim for 10min, taking out Dysosma versipellis with roots, directly placing the Dysosma versipellis with roots on silt, soaking in an aqueous solution containing 2mg/L6-BA (6-benzylaminopurine) +4mg/L NAA (naphthylacetic acid) + 6% carbendazim for 10min after illumination for 2h under the condition that the illumination intensity is 400lx, taking out the Dysosma versipellis with roots, placing the Dysosma versipellis with roots on the silt again, repeating for 5 times, taking out the Dysosma versipellis with roots, directly cultivating the Dysosma versipellis with roots in the silt in a dark place, keeping the air humidity at 75%, cultivating at the environment temperature of 22 ℃ for 3d, taking out the Dysosma versipellis with roots from the cultivated silt, and cutting the Dysosma versipel; soaking the obtained small segments of fibrous roots in an aqueous solution containing 5mg/LNAA + 6% of carbendazim for 10min, taking out, directly inserting the small segments of fibrous roots into a nutrient substrate, keeping the depth at 1.5-2.5 cm, placing and culturing under the condition that the illumination intensity is 350lx, keeping the air humidity at about 75%, keeping the ambient temperature at 22 ℃, beginning to emerge buds on the small segments of fibrous roots after culturing for 26 days, and beginning to spray 1.5kg/m of an aqueous solution containing 1.5g/L of ammonium nitrate, 5g/L of potassium nitrate, 0.05g/L of glycin inositol, 0.4mg/LNAA + 2% of carbendazim every 1 day2After spraying for 14 days, one leaf on the fibrous root grows out completely. And regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 60% and the environmental temperature at 22 ℃ until the seedlings are poured, and transplanting the seedlings with bud heads into the field. According to statistics, 94.8% of the Dysosma versipellis fibrous roots have buds growing, the roots are developed, the shortest length reaches 4.5cm, and the longest length reaches 7.4 cm.
Comparative example 1
Washing fresh Dysosma versipellis with large amount of fibrous roots with water for three times, soaking in 2mg/L6-BA +4mg/L NAA + 6% carbendazim water solution for 10min, taking out Dysosma versipellis with roots, directly placing on silt, illuminating for 2h under the condition that the illumination intensity is 400lx, soaking in 2mg/L6-BA +4mg/LNAA + 6% carbendazim water solution for 10min, taking out Dysosma versipellis with roots, placing on silt in a dark place, repeating for 5 times, taking out Dysosma versipellis with roots, directly planting in the silt in a dark place, maintaining the air humidity at 75%, culturing at 22 deg.C for 3d (day), taking out Dysosma versipellis with roots from the cultured silt, soaking in 5mg/LNAA + 6% carbendazim water solution for 10min, taking outDirectly covering with nutrient medium, culturing under the condition of illumination intensity of 350lx with air humidity of about 75%, and environment temperature of 22 deg.C, spraying 1.5g/L ammonium nitrate, 5g/L potassium nitrate, 0.05g/L glycinol, 0.4mg/L NAA, and 2% aqueous solution of carbendazim every 1d after culturing for 26d2After spraying for 14 days, new sprouts grow on the original sprouts, the number of the sprouts is 5, no sprouts are found on the Dysosma versipellis fibrous roots, but the newly grown new roots reach 10.2cm in length.
Comparative example 2
Washing fresh Dysosma versipellis with a large amount of fibrous roots with water for three times, cutting the Dysosma versipellis fibrous roots into small segments of fibrous roots with the length of 4-6 cm by using scissors, soaking the small segments of fibrous roots into an aqueous solution containing 2mg/L6-BA +4mg/LNAA + 6% carbendazim for 10min, taking out the small segments of the fibrous roots of Dysosma versipellis, placing the small segments of the fibrous roots of Dysosma versipellis on a sediment bed, illuminating the small segments of the fibrous roots of Dysosma versipellis for 2h under the illumination intensity of 400lx, soaking the small segments of the fibrous roots of Dysosma versipellis in an aqueous solution containing 2mg/L6-BA +4mg/LNAA + 6% carbendazim for 10min, repeating the steps for 5 times, taking out; soaking the obtained small segments of fibrous roots in an aqueous solution containing 5mg/L NAA and 6% carbendazim for 10min, taking out, directly inserting the small segments of fibrous roots into a nutrient substrate, keeping the depth at 1.5-2.5 cm, placing and culturing under the condition that the illumination intensity is 350lx, keeping the air humidity at about 75%, culturing at the ambient temperature of 22 ℃ for 26 days, and spraying 1.5kg/m of an aqueous solution containing 1.5g/L of ammonium nitrate, 5g/L of potassium nitrate, 0.05g/L of glycine inositol, 0.4mg/LNAA and 2% of carbendazim every 1 day2After spraying for 14 days, one leaf on the fibrous root grows out completely. And regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 60% and the environmental temperature at 22 ℃ until the seedlings are poured, and transplanting the seedlings with bud heads into the field. According to statistics, 47.1 percent of the Dysosma versipellis fibrous roots have buds growing, the roots are developed, the shortest length reaches 3cm, and the longest length reaches 5.5 cm.
Comparative example 3
Washing fresh Dysosma versipellis with large amount of fibrous root with water for three times, cutting the Dysosma versipellis fibrous root with scissors into 4-6 cm long small segments of fibrous root, and adding 2mg/L6-BA +4mg/LNAA + 6% carbendazimSoaking in water solution for 10min, taking out small segments of radix et rhizoma Dysosmatis, directly placing on a sediment bed, directly soaking in water solution containing 2mg/L6-BA +4mg/LNAA + 6% carbendazim for 10min after 2h of illumination under the illumination intensity of 400lx, repeating for 5 times, removing the soaking solution, placing at the air humidity of 75%, culturing at 22 deg.C for 3d, soaking in water solution containing 5mg/LNAA + 6% carbendazim for 10min, culturing at the illumination intensity of 350lx, maintaining the air humidity at 75%, culturing at 22 deg.C for 26d, and spraying water solution containing 1.5g/L ammonium nitrate, 5g/L potassium nitrate, 0.05g/L glycine inositol, 0.4mg/LNAA + 2% carbendazim for 1.5kg/m every 1d2After spraying for 14 days, one leaf on the fibrous root grows out completely. And regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 60% and the environmental temperature at 22 ℃ until the seedlings are poured, and transplanting the seedlings with bud heads into the field. According to statistics, 31.4% of the Dysosma versipellis fibrous roots have buds growing, the root system is developed, the shortest length reaches 3cm, and the longest length reaches 6.8 cm.
Comparative example 4
Washing fresh Dysosma versipellis with a large amount of fibrous roots with water for three times, cutting the Dysosma versipellis fibrous roots into small segments of fibrous roots with the length of 4-6 cm by using scissors, placing the small segments of fibrous roots into an aqueous solution containing 2mg/L6-BA +4mg/LNAA + 6% carbendazim, soaking for 10min, taking out the small segments of fibrous roots of Dysosma versipellis, directly placing the small segments of fibrous roots on a nutrient matrix seedbed (after disinfection), illuminating for 2h under the condition that the illumination intensity is 400lx, taking out the small segments of fibrous roots, placing the small segments of fibrous roots into an aqueous solution containing 2mg/L6-BA +4mg/LNAA + 6% carbendazim, soaking for 10min, repeating for 5 times, taking out and cutting on the nutrient matrix seedbed, keeping the air humidity at 75%, culturing at the ambient temperature of 22 ℃ for 3d, soaking for 10min by using an aqueous solution containing 5mg/LNAA + 6% carbendazim, taking out the cutting on the nutrient matrix seedbed, the air humidity is kept at about 75%, the ambient temperature is 22 ℃, after culturing for 26 days, 1.5kg/m of 1.5g/L ammonium nitrate, 5g/L potassium nitrate, 0.05g/L glycinol, 0.4mg/L NAA and 2% carbendazim aqueous solution are sprayed every 1 day2And after spraying for 14 d. Spraying foliar fertilizer regularly every 1 week, keeping air humidity at 60%, and performing sprayingThe ambient temperature is 22 ℃ until the seedlings are poured, and the seedlings with bud heads are transplanted to a field. According to statistics, 25.9% of the Dysosma versipellis fibrous roots have buds growing out, the roots are not developed, the shortest ones just grow out, and the longest ones only reach 4 cm.
Example 2
Washing fresh dysosma versipellis with a large number of fibrous roots with water for three times, putting the fresh dysosma versipellis into an aqueous solution containing 4 mg/L6-BA +6mg/L NAA + 8% carbendazim, soaking for 5min, taking out the dysosma versipellis with the fibrous roots, directly placing the dysosma versipellis on silt, illuminating for 2h under the condition that the illumination intensity is 350lx, putting the dysosma versipellis with the fibrous roots into an aqueous solution containing 4 mg/L6-BA +6mg/LNAA + 8% carbendazim, soaking for 5min, taking out the dysosma versipellis with the fibrous roots, placing the dysosma versipellis on the silt, repeating for 6 times, taking out the dysosma versipellis with the fibrous roots, directly planting the dysosma versipellis with the fibrous roots in the silt in the dark place, keeping the air humidity at 80%, culturing the environment temperature; soaking the obtained small segments of fibrous roots in an aqueous solution containing 6mg/LNAA + 8% of carbendazim for 10min, taking out the small segments of fibrous roots, directly inserting the small segments of fibrous roots into a nutrient substrate, keeping the depth of the small segments of fibrous roots at 1.5-2.5 cm, placing and culturing under the condition that the illumination intensity is 400lx, keeping the air humidity at 80%, keeping the ambient temperature at 24 ℃, beginning to emit buds on the small segments of fibrous roots after culturing for 24 days, and beginning to spray 1.5kg/m of an aqueous solution containing 2g/L of ammonium nitrate, 6g/L of potassium nitrate, 0.04g/L of glycine, 0.3g/L of inositol, 0.5mg/LNAA and 3% of carbendazim every 1 day2After spraying for 13 days, one leaf on the fibrous root grows out completely. And regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 65% and the environmental temperature at 24 ℃ until the seedlings are poured, and transplanting the seedlings with bud heads into the field. According to statistics, 92.6 percent of the Dysosma versipellis fibrous roots have buds growing, the roots are developed, the shortest length reaches 5.2cm, and the longest length reaches 8.1 cm.
Claims (3)
1. A method for quickly cultivating dysosma versipellis seedlings comprises the following specific steps:
(1) washing fresh dysosma versipellis with a large number of fibrous roots with water for two to three times, soaking in an induction liquid for 5-10 min, taking out the dysosma versipellis with the roots, directly placing on silt, after illuminating for 2-3 h under the condition that the illumination intensity is 300-;
(2) directly cultivating the Dysosma versipellis with roots treated in the step (1) in the sand in the dark, keeping the air humidity at 70-85%, and the environmental temperature at 20-25 ℃, and cultivating for 2-3 d;
(3) taking the Dysosma versipellis with roots treated in the step (2) out of the sediment, and cutting the Dysosma versipellis fibrous roots into small segments of fibrous roots with lengths of 4-6 cm by using scissors;
(4) soaking the obtained small segments of fibrous roots in a treatment solution for 3-10 min, taking out, directly inserting the small segments of fibrous roots into a nutrient substrate, keeping the depth at 1.5-2.5 cm, placing and culturing under the condition that the illumination intensity is 300 and 500lx, keeping the air humidity at 70-85%, keeping the environmental temperature at 20-25 ℃, and culturing for 15-30 d, wherein the formula of the treatment solution is 3-8 mg/LNAA + 6-10% of carbendazim aqueous solution;
(5) when the bud heads emerge from the fibrous roots of the small segments, spraying 1-2.5 kg/m of the fertilizer at intervals of 1 day2A nutrient solution until one leaf grows out completely, wherein the formula of the nutrient solution is 1-3 g/L ammonium nitrate, 5-10 g/L potassium nitrate, 0.01-0.1 g/L glycine, 0.1-0.4 g/L inositol, 0.1-0.6 mg/LNAA and 2-4% carbendazim aqueous solution;
(6) and regularly spraying a foliar fertilizer every 1 week, keeping the air humidity at 50-70% and the environmental temperature at 20-25 ℃ until the seedlings are poured, and taking the seedlings with bud heads to transplant into the field.
2. The method of claim 1 wherein said nutrient substrate is a water-retaining substrate such as a moss substrate or a coconut coir substrate.
3. The method of claim 1, wherein the foliar fertilizer is purchased directly from the market, or can be replaced by 0.5-2 g/L potassium dihydrogen phosphate.
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