CN111631997B

CN111631997B - Composition containing compound extracts of kochia scoparia and cnidium fruit and application thereof

Info

Publication number: CN111631997B
Application number: CN202010554281.0A
Authority: CN
Inventors: 邝小云; 姚艳明; 林青霞
Original assignee: Guangdong Bawei Biotechnology Co ltd
Current assignee: Guangdong Bawei Biotechnology Co ltd
Priority date: 2020-06-17
Filing date: 2020-06-17
Publication date: 2022-04-26
Anticipated expiration: 2040-06-17
Also published as: CN111631997A

Abstract

The invention discloses a composition containing compound extracts of Kochia scoparia and cnidium fruit and application thereof, aiming at providing a composition which realizes the synergy of all components, realizes the mite removal and inhibition by controlling sebum secretion, inhibiting bacterial proliferation, killing mites and other ways, and mainly comprises the following components in parts by weight: 5-10 parts of compound extracts of kochia scoparia and cnidium fruit, 6-15 parts of extracts of folium ramosiae, 2-9 parts of extracts of clerodendranthus spicatus, 30-40 parts of polyhydric alcohol and 34-44 parts of water; belongs to the technical field of daily chemicals.

Description

Composition containing compound extracts of kochia scoparia and cnidium fruit and application thereof

Technical Field

The invention discloses a composition, in particular to a composition containing compound extracts of kochia scoparia and cnidium fruit, belonging to the technical field of daily chemicals.

Background

The mites parasitizing the human body mainly comprise two types, namely, a folliculus acaridae (Demodexfoliolorum) and a lipopenidae (Dbrevis), the mites are slender and worm-shaped, a palate body and a point are arranged on the front part of a body with the body length of about 0.1-0.2 mm, and the whole limb is in a short needle shape. The folliculorvarus is usually parasitized in hair follicles of the nose, the eye and the face and other parts, and is often concentrated in one part of many individuals, and the parasitized hair follicles are often enlarged. Many of the mites grow in the sebaceous glands, and are usually present individually, which may cause the sebaceous glands to secrete excessive amounts. A plurality of research data show that the infection rate of human mites in China is high and is reported to reach more than 98 percent at most. Because the human body has no immunity to the infection of the acarid, the acarid can be infected by different ages, different nationalities and different sexes.

Recent studies suggest that: the human body skin mites have close relationship with the problems on the human body face, such as folliculitis, seborrheic dermatitis, alopecia, blepharitis, external auditory canal pruritus and the like, particularly with the generation of acne, pox and rosacea. The mites mostly parasitize in hair follicle sebaceous glands, absorb nutrient substances in cells and sebaceous gland secretions to breed in a large quantity, bacteria on the surface of skin are brought into the hair follicles and the hair sebaceous glands when the mites breed, normal cells are damaged, and the parasitic parts can cause hair follicle enlargement, blood vessel expansion, fibrous tissue hyperplasia and the like due to the mechanical stimulation of polypide and the chemical stimulation of polypide excrement, so that the skin has the phenomena of red spots, papules, granuloma, pustules, acne, pruritus and the like, and the occurrence of dermatophytosis also relates to the condition that the mites and the bacteria on the surface of the skin are infected together, thereby further aggravating skin anaphylactic reaction and harming the skin health and the psychological health of people. With the continuous improvement of living standard of people, people pay more and more attention to facial beauty and mental health problems, and the prevention and treatment of skin mites gradually become a problem to be solved urgently.

At present, mite killing care products on the market are not good, some products can achieve certain effect by using chemical medicine combination to kill mites, but have irritation to skin and cannot be used for a long time, and some products only have single mite killing way, only inhibit mites from aspects of regulating sebum secretion or inhibiting bacterial breeding and the like, and have unsatisfactory mite killing effect. Therefore, a composition for removing mites, which is purely natural, safe, non-irritant and remarkable in effect, needs to be developed.

Disclosure of Invention

In view of the above disadvantages, the present invention aims to provide a composition containing a compound extract of kochia scoparia and cnidium officinale fruit, which can achieve the effects of mite removal and mite inhibition by controlling sebum secretion, inhibiting bacterial proliferation, killing mites and other ways, and can prevent skin mites from breeding, clean hair follicles and skin, improve skin symptoms such as erythema, acne and pruritus caused by mites and bacteria, and achieve a healthy skin state when applied to daily products.

Therefore, the first technical scheme provided by the invention is as follows:

a composition containing compound extracts of Kochia scoparia and cnidium fruit mainly comprises the following components in parts by weight: 5-10 parts of compound extracts of belvedere fruit and cnidium fruit, 6-15 parts of extracts of folium orostachydis scholaris, 2-9 parts of extracts of clerodendranthus spicatus, 30-40 parts of polyhydric alcohol and 34-44 parts of water.

Wherein, the Kochia scoparia fruit contains various active ingredients such as triterpenes, glycosides, alkaloids, flavonoids compounds and the like, has different degrees of inhibitory action on various dermatophytes and bacteria, and has the effect of relieving itching.

The cnidium fruit contains chemical components such as cnidium lactone, bornyl acetate, isovaleric acid borneol ester and the like, has the effects of killing insects, resisting fungi, dispelling wind and relieving itching and the like, is also a biological acarus-inhibiting agent, can effectively remove mites, prevent the regeneration of the mites, inhibit seborrheic dermatitis, control oil, prevent the formation of pox, eliminate redness and swelling and smoothen the skin.

The extract of the leaves of the Chinese ash tree is rich in active ingredients such as coriander glucoside, can reduce the secretion of grease from the source, fundamentally solves the problem of large pores, and achieves the effect of smoothing and smoothing the skin.

The clerodendranthus spicatus extract can effectively inhibit the activity of 5 alpha-reductase, reduce sebum secretion, prevent sebum oxidation and achieve a good oil control effect.

Furthermore, the composition containing the compound extracts of the fructus kochiae and the fructus cnidii also comprises 3 to 8 parts of vitex negundo leaf extract. The folium Viticis negundo extract is rich in flavone components such as vitexin, has strong antibacterial and bactericidal effects on various dermatophytes and bacteria, and has acarid killing effect.

Furthermore, the mass ratio of the folium orostachydis radicis extract to the clerodendranthus spicatus extract in the composition containing the compound extracts of the kochia scoparia and the cnidium fruit is 4: 3.

Furthermore, in the composition containing the compound extracts of the kochia scoparia and the cnidium fruit, the polyalcohol is one or a combination of more of propylene glycol, butanediol, 1, 2-pentanediol, glycerol and 1, 2-hexanediol.

Further, the preparation method of the compound extract of kochia scoparia and cnidium fruit in the composition comprises the following steps:

(1) pretreatment of raw materials: respectively pulverizing dried Kochia scoparia and cnidium fruit, sieving with 40-70 mesh sieve, and mixing;

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the material-liquid ratio of 1:10-1:15(g: mL), adding complex enzyme with the weight of 0.2-0.4% of the mixed powder, stirring for 60-80min under the conditions of pH4.0-5.0 and the temperature of 45-55 ℃, and heating to boil to inactivate enzyme;

(3) pressure extraction: adding absolute ethanol into the enzymolysis liquid in the step (2) to obtain 50-70% ethanol solution, placing the ethanol solution in a high-temperature high-pressure reaction container, wherein the pressure is 0.4-0.7MPa, the temperature is 70-90 ℃, and the extraction time is 25-35 min;

(4) and (3) purification: filtering the extracting solution obtained in the step (3), concentrating under reduced pressure to 1/6 of the original volume, passing the concentrated solution through an AB-8 macroporous adsorption resin column, eluting with 3 times of column volume of water, eluting with 4 times of column volume of 10% ethanol solution, discarding the eluent, eluting with 3-6 times of column volume of 40-70% ethanol solution at an elution rate of 1.0-2.0BV/h, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the compound extract of kochia fruit and cnidium fruit.

Further, the mass ratio of the belvedere fruit to the cnidium fruit in the step (1) of the preparation method is 1: 3.

Further, in the step (2) of the preparation method, the complex enzyme consists of mannose polymerase with enzyme activity of 30000u/g and xylanase with enzyme activity of 50000u/g, and the mass ratio of the mannose polymerase to the xylanase is 2: 1.

In the composition, in order to increase the stability of the composition, 0.1-1 part by weight of preservative can be added into the composition, and the preservative can be one or a combination of more of conventional preservatives such as phenoxyethanol, sodium benzoate, ethylhexylglycerin and the like.

The second technical scheme provided by the invention is as follows:

the composition containing the compound extract of the kochia scoparia and the cnidium fruit is applied to preparation of daily necessities, wherein the daily necessities are one of water aqua, emulsion, cream, gel, powder, spray, essence, washing and caring, handmade soap and facial mask.

In the production process, the selection and preparation process of other components in the daily necessities can be carried out according to the conventional operation in the field, wherein the mass percent of the composition in the daily necessities is 0.2-10%.

Compared with the prior art, the invention has the following beneficial effects:

(1) the composition provided by the invention realizes the purposes of removing and inhibiting mites by controlling sebum secretion, inhibiting bacterial proliferation on the surface of skin, killing mites and the like by utilizing the synergistic effect of the compound extracts of Kochia scoparia and cnidium monnieri, the extract of the leaf of the Amur brier fruit and the extract of the clerodendrum spicatum, and the extract has a remarkable effect, can obviously improve skin problems of large skin pores, acne rosacea, folliculitis, pruritus and the like caused by strong grease secretion, mites and bacteria when being applied to daily cosmetics, does not contain chemical components, and is safe and non-irritant to use. When the Amur bracteatum leaf extract and the clerodendranthus spicatus extract are combined in a specific ratio, the capability of the composition for reducing skin oil secretion can be effectively improved, the food supply of mites is blocked, and the effect of blocking the propagation of the mites is achieved. The above composition can also be added with folium Viticis Cannabifoliae extract, and can be reasonably combined with other components to further enhance the effects of sterilizing and removing acarid, so that the problem skin can be rapidly recovered to natural health state.

(2) The compound extract of the belvedere fruit and the cnidium fruit in the composition adopts an enzymolysis-assisted pressurized solvent method to extract active ingredients in a mixture, the reaction condition is mild, the three-dimensional structure and the biological activity of a natural product are not damaged, the original drug effect of the active ingredients is favorably maintained, and particularly, when the belvedere fruit and the cnidium fruit are extracted in a specific ratio, and the mixture is subjected to the combined enzymolysis action of mannase and xylanase, the antibacterial and acaricidal activity of the composition can be remarkably improved.

Detailed Description

The following claims are hereby incorporated into the detailed description of the invention, with the understanding that the invention is not to be limited in any way, as any number of modifications may be made by one within the scope of the claims and the invention is still within the scope of the claims. The raw materials used in the present invention may be obtained by home-made or purchased from commercial sources. Wherein the extract of Hippocampus japonicus leaf is obtained from Noran Biotech, Guangzhou, and the extract of Clerodendranthus spicatus is obtained from Xinan chemical trade, Inc., of Foshan; vitex folium extract is purchased from Guangzhou sandong Biotechnology GmbH.

Example 1

The invention provides a composition containing a compound extract of kochia scoparia and cnidium fruit, which comprises the following components in parts by weight: 5g of compound extract of kochia scoparia and cnidium fruit, 15g of extract of folium orostachydis scholaris, 6g of extract of clerodendranthus spicatus, 36g of butanediol and 38g of water.

Example 2

The invention provides a composition containing a compound extract of kochia scoparia and cnidium fruit, which comprises the following components in parts by weight: 7g of compound extract of kochia scoparia and cnidium fruit, 10g of extract of folium orostachydis scholaris, 9g of extract of clerodendranthus spicatus, 30g of glycerol and 44g of water.

The kochia scoparia and cnidium fruit composite extracts described in examples 1-2 are prepared by the following steps in sequence:

(1) pretreatment of raw materials: respectively crushing the dried fructus kochiae and the dried fructus cnidii, sieving the crushed fructus kochiae and the dried fructus cnidii with a 40-mesh sieve, and mixing the crushed fructus kochiae and the dried fructus cnidii according to the mass ratio of 2:1 for later use;

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the material-liquid ratio of 1:15(g: mL), adding 0.3 wt% of the mixed powder, namely, mannose polymerase (enzyme activity of 30000u/g) and xylanase (enzyme activity of 50000u/g) in the mass ratio of 1:3, stirring for 60min at the temperature of 50 ℃ at the pH of 5.0, and heating to boil to inactivate the enzyme;

(3) pressure extraction: adding absolute ethanol into the enzymolysis liquid in the step (2) to obtain a 50% ethanol solution, and placing the ethanol solution in a high-temperature high-pressure reaction container, wherein the pressure is 0.7MPa, the temperature is 90 ℃, and the extraction time is 32 min;

(4) and (3) purification: filtering the extracting solution obtained in the step (3), concentrating under reduced pressure to 1/6 of the original volume, passing the concentrated solution through an AB-8 macroporous adsorption resin column, eluting with 3 times of column volume of water, eluting with 4 times of column volume of 10% ethanol solution, discarding the eluent, eluting with 4 times of column volume of 50% ethanol solution at an elution rate of 2.0BV/h, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the compound extract of kochia fruit and cnidium fruit.

Example 3

The invention provides a composition containing a compound extract of kochia scoparia and cnidium fruit, which comprises the following components in parts by weight: 10g of compound extract of kochia scoparia and cnidium fruit, 6g of extract of folium Rubi Bungeanae, 2g of extract of Clerodendranthus spicatus, 8g of extract of folium Viticis negundo, 30g of butanediol, 10g of 1, 2-hexanediol and 34g of water.

Example 4

The invention provides a composition containing a compound extract of kochia scoparia and cnidium fruit, which comprises the following components in parts by weight: 6g of compound extract of kochia scoparia and cnidium fruit, 9g of extract of folium ramosiae, 8g of extract of clerodendranthus spicatus, 3g of extract of folium vitex negundo, 8g of propylene glycol, 20g of butanediol, 10g of glycerol, 0.5g of phenoxyethanol and 35.5g of water.

The kochia scoparia and cnidium fruit composite extracts described in examples 3-4 are prepared by the following steps in sequence:

(1) pretreatment of raw materials: respectively crushing the dried fructus kochiae and the dried fructus cnidii, sieving with a 70-mesh sieve, and mixing according to the mass ratio of 3:2 for later use;

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the material-liquid ratio of 1:12(g: mL), adding 0.4 wt% of the mixed powder, namely, mannose polymerase (enzyme activity of 30000u/g) and xylanase (enzyme activity of 50000u/g) in the mass ratio of 1:1, stirring for 80min at the temperature of 45 ℃ at the pH of 4.5, and heating to boil to inactivate the enzyme activity;

(3) pressure extraction: adding absolute ethanol into the enzymolysis liquid in the step (2) to obtain a 70% ethanol solution, and placing the ethanol solution in a high-temperature high-pressure reaction container, wherein the pressure is 0.5MPa, the temperature is 80 ℃, and the extraction time is 25 min;

(4) and (3) purification: filtering the extracting solution obtained in the step (3), concentrating under reduced pressure to 1/6 of the original volume, passing the concentrated solution through an AB-8 macroporous adsorption resin column, eluting with 3 times of column volume of water, eluting with 4 times of column volume of 10% ethanol solution, discarding the eluent, eluting with 6 times of column volume of 40% ethanol solution at an elution rate of 1.0BV/h, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the compound extract of kochia fruit and cnidium fruit.

Example 5

The invention provides a composition containing a compound extract of kochia scoparia and cnidium fruit, which comprises the following components in parts by weight: 7g of compound extract of kochia scoparia and cnidium fruit, 10g of extract of folium Rubi Bungeanae, 4g of extract of folium orthosiphoni, 5g of extract of folium Viticis negundo, 25g of butanediol, 9g of 1, 2-pentanediol and 40g of water.

Example 6

The invention provides a composition containing a compound extract of kochia scoparia and cnidium fruit, which comprises the following components in parts by weight: 7g of compound extract of kochia scoparia and cnidium fruit, 8g of extract of folium Rubi Bungeanae, 6g of extract of folium orthosiphoni, 5g of extract of folium Viticis negundo, 25g of butanediol, 9g of 1, 2-pentanediol and 40g of water.

The kochia scoparia and cnidium fruit composite extracts described in examples 5-6 are prepared by the following steps in sequence:

(1) pretreatment of raw materials: respectively crushing the dried fructus kochiae and the dried fructus cnidii, sieving the crushed fructus kochiae and the dried fructus cnidii with a 60-mesh sieve, and mixing the ground fructus kochiae and the dried fructus cnidii in a mass ratio of 2:5 for later use;

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the material-liquid ratio of 1:10(g: mL), adding 0.2 wt% of mannose polymerase (enzyme activity of 30000u/g) and xylanase (enzyme activity of 50000u/g) in a mass ratio of 6:1 into the mixed powder, stirring for 70min at the pH of 4.0 and the temperature of 55 ℃, and heating to boil to inactivate the enzyme activity;

(3) pressure extraction: adding absolute ethanol into the enzymolysis liquid obtained in the step (2) to obtain a 60% ethanol solution, and placing the ethanol solution in a high-temperature high-pressure reaction container, wherein the pressure is 0.4MPa, the temperature is 70 ℃, and the extraction time is 35 min;

(4) and (3) purification: filtering the extracting solution obtained in the step (3), concentrating under reduced pressure to 1/6 of the original volume, passing the concentrated solution through an AB-8 macroporous adsorption resin column, eluting with 3 times of column volume of water, eluting with 4 times of column volume of 10% ethanol solution, discarding the eluent, eluting with 3 times of column volume of 70% ethanol solution at an elution rate of 1.5BV/h, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the compound extract of kochia fruit and cnidium fruit.

Example 7

The compound extract of kochia scoparia and cnidium fruit in example 7 is prepared by the following steps in sequence:

(1) pretreatment of raw materials: respectively crushing the dried fructus kochiae and the dried fructus cnidii, sieving the crushed fructus kochiae and the dried fructus cnidii with a 60-mesh sieve, and mixing the ground fructus kochiae and the dried fructus cnidii in a mass ratio of 1:3 for later use;

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the material-liquid ratio of 1:10(g: mL), adding 0.2 wt% of the mixed powder, namely, mannose polymerase (enzyme activity of 30000u/g) and xylanase (enzyme activity of 50000u/g) in the mass ratio of 2:1, stirring for 70min at the temperature of 55 ℃ at the pH value of 4.0, and heating to boil to inactivate the enzyme;

The preparation method of the composition containing the compound extracts of kochia scoparia and cnidium fruit in any one of the above examples 1 to 7 is as follows:

(1) mixing phenoxyethanol with small amount of water uniformly in advance for use (when phenoxyethanol is not present, the step is omitted);

(2) heating the residual water to 40-45 ℃, adding the polyhydric alcohol, uniformly stirring, adding the mixture obtained in the step (1) which is treated in advance, uniformly stirring, adding other materials, and uniformly stirring to obtain the water-based paint.

To demonstrate the effect of the present invention, comparative experimental examples of the present application are given below:

comparative example 1

A composition comprising the following components in parts by weight: 5g of compound extract of kochia scoparia and cnidium fruit, 21g of extract of folium orostachydis scholaris, 36g of butanediol and 38g of water.

The preparation method of the compound extract of kochia scoparia and cnidium fruit is the same as that of example 1.

Compared with example 1, only the clerodendranthus spicatus extract was replaced with the same amount of the extract of the leaf of the Rubus sibirica, and the other ingredients were the same.

Comparative example 2

A composition comprising the following components in parts by weight: 6g of the folium oroxyli extract, 2g of the clerodendranthus spicatus extract, 18g of the vitex negundo extract, 30g of butanediol, 10g of 1, 2-hexanediol and 34g of water.

In comparison with example 3, only the combined extract of kochia scoparia and cnidium fruit was replaced with the same amount of the extract of vitex negundo, and the other ingredients were the same.

Comparative example 3

A composition comprising the following components in parts by weight: 7g of compound extract of kochia scoparia and cnidium fruit, 8g of extract of folium Rubi Bungeanae, 6g of extract of folium orthosiphoni, 5g of extract of folium Viticis negundo, 25g of butanediol, 9g of 1, 2-pentanediol and 40g of water.

The compound extract of the belvedere fruit and the cnidium fruit is prepared by the following steps in sequence:

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the feed-liquid ratio of 1:10(g: mL), adding 0.2% of xylanase (enzyme activity of 50000u/g) by weight of the mixed powder, stirring for 70min at the pH of 4.0 and the temperature of 55 ℃, and heating to boil to inactivate the enzyme activity;

In comparison with example 6, the composition had the same raw material composition. But the compound extract of the kochia scoparia and the cnidium fruit is only added with xylanase for enzymolysis in the step (2) during preparation.

Comparative example 4

A composition comprising the following components in parts by weight: 2g of kochia scoparia extract, 5g of cnidium fruit extract, 8g of folium rambutae, 6g of clerodendranthus spicatus extract, 5g of vitex negundo leaf extract, 25g of butanediol, 9g of 1, 2-pentanediol and 40g of water.

The above Kochia scoparia extract and cnidium officinale extract are commercially available. Kochia scoparia extract and cnidium officinale extract were obtained from Lebo cosmeceutical technology (Shanghai) Co., Ltd.

Comparative examples 1-4 the compositions of any one of the groups were prepared in the same manner as in the examples.

The composition containing the compound extracts of the kochia scoparia and the cnidium fruit can be used as an active ingredient for preparing daily necessities for killing and inhibiting mites. When the composition is used specifically, the composition prepared by the invention is directly added into a daily necessity matrix, and the addition amount is 0.2-10 wt%.

To better illustrate the advantages of the present invention, the efficacy test of the composition containing the compound extract of kochia scoparia and cnidium fruit provided by the present invention is given below:

test 1: skin irritation test

(1) Test samples: compositions prepared in examples 1-7.

(2) The test method comprises the following steps: 30 healthy volunteers are selected, the age is 18-45 years, the test subjects meet the volunteer selection standard, no history of allergic dermatitis exists, and no irritant product is used in the previous month.

Taking about 0.020-0.025 ml of a test sample, coating the test sample in a small chamber of a spot tester (Beijing science popularization instrument factory), applying a special adhesive tape for external application on the back of a testee, removing the spot tester after 24 hours, slightly wiping off the residual product by using a wet paper towel after the residual product exists, and recording the result according to the skin reaction grading standard in technical Specification for cosmetic safety (2015) at 0.5 hour, 24 hours and 48 hours respectively. The identification criteria are shown in Table 1.

TABLE 1 identification standards

Grade	Identification criteria
		0	Has no irritation and erythema
1	Mild erythema
		2	Erythema
3	Erythema, pimples, blisters
		4	Severe edema and bulla

As can be seen from the results of the closed patch test, 30 subjects showed no adverse reaction and no erythema, burning sensation, itching, etc. at 0.5 hour, 24 hours, and 48 hours on the compositions containing the compound extracts of Kochia scoparia and cnidium fruit prepared in examples 1 to 7. The composition of the invention is safe and mild to use and has no stimulation to skin.

And (3) testing 2: efficacy test for inhibition of sebum secretion

(1) Test samples: the compositions of examples 1 to 7 and comparative examples 1 to 4 were added to the following cleanser bases, respectively, in an amount of 2 wt%, to obtain cosmetics containing the compositions of examples 1 to 7 and comparative examples 1 to 4, which were designated as cleansers 1 to 7, comparative cleansers 1 to 4, respectively, and the cleanser bases as controls.

Phase A: 4g of lauric acid, 6g of stearic acid, 5g of palmitic acid, 3g of myristic acid, 2g of ethylene glycol distearate, 2.5g of white beeswax, 62g of ceteareth-62, 1.0g of PEG-150 distearate and a proper amount of preservative;

phase B: 10g of glycerol, 5g of polyethylene glycol-4005 g of butanediol, 3g of sodium laureth sulfate, 0.5g of hydroxyethyl cellulose// sodium acetate// water// isopropanol// cellulose, 0.3g of disodium EDTA and adding water to 100 g;

and C phase: 2.5g of potassium hydroxide and 6g of water;

phase D: 5g of cocoyl sodium glycinate, 3g of sodium lactate and 10g of water;

phase E: 2g of the compositions of examples 1-7 or comparative examples 1-4, 0.3g of polyethylene and a proper amount of essence.

The preparation methods of the facial cleanser 1-7 and the comparative facial cleanser 1-4 are as follows:

firstly, adding the phase A raw material into a stirring pot, stirring and heating to 85 ℃, and uniformly stirring and dispersing for later use;

secondly, adding the phase B raw material into a vacuum emulsifying pot, stirring and heating to 85 ℃, adding the phase C raw material, homogenizing for 3 minutes, and keeping the temperature for 30 minutes until all the materials are dissolved;

slowly filtering the raw materials in the step I, pumping the raw materials into the vacuum emulsifying pot in the step II, keeping the temperature in vacuum for stirring for 1.5 hours after pumping, and cooling after defoaming is completed;

cooling to 60 ℃, adding the D-phase raw material which is mixed and dispersed uniformly in advance, and stirring uniformly in vacuum at a low speed;

and fifthly, cooling to 40 ℃, adding the E-phase raw material, and uniformly stirring.

(2) The test method comprises the following steps: selecting 60 subjects with the ages of 18-45 years and the basic value of the oil content in the forehead test area which is larger than 180 (namely oily or mixed oily skin subjects), randomly dividing the subjects into 12 groups, measuring the oil content of the forehead by using a skin oil test probe sebumeterSM815 before using the product and on the 28 th day of using the product respectively by 5 persons in each group, measuring the oil content of the forehead twice in each area, and averaging; during the test period, the test subjects used the test sample face-cleaning once a day, each group of subjects was prescribed to use 0.6g of sample each time, and the test environment temperature was: 20-22 ℃, humidity is as follows: 40-60%. The results are shown in Table 2.

TABLE 2 measurement results of sebum secretion inhibition

The experimental results in table 2 show that the reduction of skin oil is significantly higher than that of the comparative facial cleanser after the facial cleanser prepared by the composition of the invention is continuously used for 28 days, which shows that the components of the composition of the invention act synergistically to inhibit the secretion of skin oil more effectively, clear pores and achieve a good oil control effect. The oil control effect of the facial cleanser 6 is superior to that of the facial cleanser 5, and the result shows that when the clerodendranthus spicatus extract and the folium orostachydis sieboldii extract are compounded in a specific ratio, the effect of inhibiting sebum secretion is better, so that the food supply of mites can be greatly reduced, and the propagation speed of the mites is reduced.

And (3) testing: test for bacteriostatic efficacy

Staphylococcus aureus is the most common pathogenic bacterium in human pyogenic infection, and is gram-positive as Staphylococcus epidermidis growing on the surface of organisms. As can be seen from the mechanism of demodex disease development, these two bacteria are present in the skin of skin lesions, with 94.3% staphylococcus epidermidis and 1.1% staphylococcus aureus. The bacteriostatic efficacy of the compositions of the present invention was evaluated in this experiment by determining the inhibitory rate of the compositions against staphylococcus epidermidis and staphylococcus aureus.

(1) Test samples: the face washes 1, 3, 5, 6, 7 in test 2 were used, and the face washes 1, 3, 4 were compared.

(2) The test method comprises the following steps: in order to verify the antibacterial effect of the composition, the antibacterial performance of the composition is determined according to GB15979-2002 hygienic Standard for Disposable sanitary articles appendix C4 'test method for antibacterial performance of dissolution antibacterial (bacteriostatic) product'.

Preparation of bacterial suspension: washing 24h culture of Staphylococcus epidermidis and Staphylococcus aureus with 0.03mol/L phosphate buffer solution to obtain 10⁴～10⁶CFU/mL bacterial suspension;

respectively taking 1g of test sample, adding 0.1mL of the bacterial suspension, uniformly coating and mixing, starting timing, adding 9mL0.03mol/L of phosphate buffer solution after 20min, fully and uniformly mixing, taking 3 dilutions, respectively taking 1mL of the dilutions, placing the dilutions in two parallel plates, adding corresponding culture medium, and culturing for 24 hours at 36 +/-1 ℃ under an anaerobic condition to count bacterial colonies. The above experiment was repeated 3 times, and the average was taken and a blank control group was set.

The calculation formula of the bacteriostatic rate is as follows: and X is (A-B)/A X100%, wherein X is the bacteriostasis rate, A is the average recovered colony of the control sample, and B is the average recovered colony of the test sample.

The test results are shown in table 3.

TABLE 3 results of the bacteriostatic efficacy test

The experimental results in table 3 show that the facial cleanser containing the composition has strong bacteriostatic effect on staphylococcus epidermidis and staphylococcus aureus, while the bacteriostatic rate of the comparative facial cleanser is lower than that of the facial cleansers in the examples, which shows that the components of the composition cooperate with each other to exert the best bacteriostatic action and prevent the combined infection of mites and bacteria on the surface of the skin.

And (4) testing: mite eradication efficacy testing

(1) Test samples: the sample from test 3 was still used.

(2) The test method comprises the following steps: and performing mite expelling and killing tests according to the industry standard NY/T1151.2-2006. Wherein, the test mite species: dust mites (dermatophagoidesfarina), hermaphrodite and nymphs. The evaluation indexes of the drug effect are shown in a table 4, and the test results of the mite expelling and killing are shown in a table 5.

TABLE 4 evaluation index of drug efficacy

TABLE 5 Deacarid and Deacarid test results

Sample (I)	Mite-repelling rate	Mite eradication rate
			Face cleanser 1	90.82％	95.06％
Face cleanser 3	91.56％	95.91％
			Face cleanser 5	93.67％	96.74％
Face cleanser 6	96.45％	98.53％
			Face cleanser 7	97.90％	100％
Comparative face cleanser 1	70.34％	75.53％
			Comparative face cleanser 3	73.25％	79.41％
Comparative face cleanser 4	78.12％	84.46％

As can be seen from the experimental results in Table 5, both the mite-expelling rate and the mite-killing rate of the facial cleanser containing the composition can reach B level, and the mite-expelling and mite-killing effects of the facial cleanser containing the composition are obviously superior to those of the facial cleanser in the comparative example. Particularly, when the mixture of the cnidium fruit and the kochia scoparia fruit is compounded in a specific proportion, and under the combined enzymolysis action of the mannose polymerase and the xylanase, the compound extract of the cnidium fruit and the kochia scoparia fruit is cooperated with other components, and the mite expelling and killing performance of the composition is obviously improved.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. A composition containing a compound extract of Kochia scoparia and cnidium fruit is characterized by mainly comprising the following components in parts by weight: 5-10 parts of compound extracts of kochia scoparia and cnidium fruit, 6-15 parts of folium ramosiae, 2-9 parts of clerodendranthus spicatus, 3-8 parts of vitex negundo, 30-40 parts of polyhydric alcohol and 34-44 parts of water;

the preparation method of the compound extract of the belvedere fruit and the cnidium fruit comprises the following steps:

(1) pretreatment of raw materials: respectively pulverizing dried Kochia scoparia and cnidium fruit, sieving with 40-70 mesh sieve, and mixing to obtain mixed powder;

(2) enzymatic hydrolysis: adding water into the mixed powder in the step (1) according to the material-liquid ratio of 1:10-1:15/g: mL, adding complex enzyme accounting for 0.2-0.4% of the weight of the mixed powder, stirring for 60-80min at the pH of 4.0-5.0 and the temperature of 45-55 ℃, and heating to boil to inactivate enzyme;

2. The composition containing the compound extract of kochia scoparia and cnidium fruit as claimed in claim 1, wherein the mass ratio of the extract of the leaves of the Amur buster fruit to the extract of the clerodendranthus spicatus is 4: 3.

3. The composition containing the compound extract of kochia scoparia and cnidium fruit as claimed in claim 1, wherein the polyhydric alcohol is one or more of propylene glycol, butylene glycol, 1, 2-pentanediol, glycerol and 1, 2-hexanediol.

4. The composition containing the compound extract of kochia scoparia and cnidium fruit as claimed in claim 1, wherein the mass ratio of kochia scoparia to cnidium fruit in step (1) is 1: 3.

5. The composition containing the compound extract of kochia scoparia and cnidium fruit as claimed in claim 1, wherein the compound enzyme in step (2) comprises mannosidase with enzyme activity of 30000IU/g and xylanase with enzyme activity of 50000IU/g, and the mass ratio of the mannosidase to the xylanase is 2: 1.

6. Use of the composition comprising the complex extract of kochia scoparia and cnidium fruit as set forth in any one of claims 1 to 5 for the preparation of a daily necessity.

7. The use of the composition containing the compound extract of kochia scoparia and cnidium monnieri fruit as claimed in claim 6 in the preparation of daily necessities, wherein the daily necessities are one of water aqua, lotion, cream, jelly and powder.