CN111631171B - Large-scale Hippocampus grey sea horse breeding method based on biological floc principle - Google Patents

Large-scale Hippocampus grey sea horse breeding method based on biological floc principle Download PDF

Info

Publication number
CN111631171B
CN111631171B CN202010498650.9A CN202010498650A CN111631171B CN 111631171 B CN111631171 B CN 111631171B CN 202010498650 A CN202010498650 A CN 202010498650A CN 111631171 B CN111631171 B CN 111631171B
Authority
CN
China
Prior art keywords
culture
hippocampus
seedlings
water
sea horse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010498650.9A
Other languages
Chinese (zh)
Other versions
CN111631171A (en
Inventor
张东
林听听
刘鑫
辛茂盛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Zhongyuan Biotechnology Co ltd
East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Original Assignee
Qingdao Zhongyuan Biotechnology Co ltd
East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Zhongyuan Biotechnology Co ltd, East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences filed Critical Qingdao Zhongyuan Biotechnology Co ltd
Priority to CN202010498650.9A priority Critical patent/CN111631171B/en
Publication of CN111631171A publication Critical patent/CN111631171A/en
Application granted granted Critical
Publication of CN111631171B publication Critical patent/CN111631171B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/13Prevention or treatment of fish diseases
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to a large-scale gray hippocampus culture method based on a biological floc principle. The method comprises the following steps: brushing the wall of the cultivated container to be darker; after the Hippocampus erectus seedlings are put, adding a carbon source and beneficial bacteria into a culture container; controlling light during the breeding period of the Hippocampus Grosvenorii fry. The method can reduce the disease incidence rate, especially enteritis, reduce the labor intensity, keep the water quality stable, save the culture water, not only improve the culture effect of normal seedlings, but also improve the culture effect of low-quality seedlings, the survival rate of high-quality seedlings in summer reaches more than 60%, the survival rate of low-quality seedlings reaches more than 40%, and the culture benefit is greatly improved.

Description

Large-scale Hippocampus grey sea horse breeding method based on biological floc principle
Technical Field
The invention belongs to the field of sea horse cultivation, and particularly relates to a large-scale grey sea horse cultivation method based on a biological floc principle.
Background
The hippocampus is a traditional rare Chinese medicine. According to statistics, the amount of dry medicine used in China is more than 300 tons every year. Because natural resources in China are exhausted recently, most of the sea horses required by the market at present are imported, the price is increased year by year, the average price exceeds 7000 yuan per kilogram, and the value exceeds 21 billion yuan according to 300 tons imported per year. Because natural resources of the sea horses are seriously damaged, artificial breeding research is carried out in many countries in the world for recovering the resources. In recent five years, the Hippocampus culture technology taking Hippocampus erectus as a leading variety in China is developed rapidly, but the large-scale production technology still has many problems, and low quality seedlings and low culture survival rate in summer are one of the main problems to be solved urgently at present.
The sea horse can be bred for many times in one year, and in nature, the breeding season is generally spring and autumn, mainly spring. Under artificial conditions, the seedlings can be bred in four seasons, but the quality of the seedlings is reduced in summer or after continuous breeding for multiple times. The immunity of the low-quality seedlings is reduced, and the bacteria are quickly propagated due to high temperature in summer, so that enteritis and other bacterial diseases are easy to outbreak during the culture period in summer, and the full army of hippocampus can be induced to submerge. Although temperature reduction is a technical option in summer, the effect is not ideal, and the large-scale culture cost is high, so that how to improve the survival rate of the young Hippocampus japonicus in summer is one of the important problems to be solved in the current Hippocampus japonicus culture.
The biological flocculation technology is a technology which is characterized in that organic carbon substances are added into a water body by controlling a water body nutrition structure, the C/N ratio in the water body is adjusted, the propagation of heterotrophic bacteria in the water body is promoted, the inorganic nitrogen is assimilated by microorganisms, culture metabolites such as ammonia nitrogen and the like in the water body are converted into bacteria self-components, and the bacteria are flocculated into granular substances to be ingested by cultured animals, so that the effects of maintaining the water environment stable, reducing water change amount, improving the immunity of the animals, improving the culture survival rate, increasing the yield, reducing the feed coefficient and the like are achieved. However, biological flocks are not suitable for all the objects to be cultivated and have high technical requirements, in particular, the requirement of large air inflation amount and no dead angle. The sea horse is small in size and weak in swimming ability, and the inflation is not suitable for sea horse cultivation. In addition, the biological floc water for large-scale animal culture is required to form a real floc which can be ingested by a culture object, the sea horse is different from common fishes, and the excessive floc in the floc water is possibly not suitable for the survival of the sea horse. Therefore, the standard biological floc technology is not suitable for the sea horse, but the principle can be used for culturing sea horse culture water so as to achieve the purposes of improving the immunity of the sea horse, reducing the disease incidence and improving the culture survival rate.
Disclosure of Invention
The invention aims to solve the technical problem of providing a large-scale hippocampus grey sea horse breeding method based on a biological floc principle so as to overcome the defects of high incidence rate and low survival rate of hippocampus grey sea horse enteritis diseases during summer high-temperature breeding in the prior art.
The inventor finds that the cultivation effect is better than that of only adding beneficial bacteria by properly adding a carbon source and beneficial bacteria in the sea horse cultivation water, particularly, the cultivation survival rate is remarkably improved and enteritis is less in incidence when the water temperature is higher than 26 ℃. The invention is obtained on the basis of full experiments.
The invention provides a large-scale gray hippocampus culture method based on a biological floc principle, which comprises the following steps:
(1) brushing the wall of the culture container to be darker, wherein the light reflectivity of the dark color is 15-20%;
(2) when the young Hippocampus erectus is released, simultaneously adding carbon source and EM (effective microorganisms) according to the proportion of 5-6 g/ton and 6-7 ml/ton respectively, wherein the water level is 30-35 cm, and then supplementing new water every day until the water level is gradually increased to 60-70 cm; continuously adding a carbon source and EM (effective microorganisms) for 6-7 days after seedling placement, and adding every other day;
(3) the illumination is controlled during the breeding period of the hippocampus griseus seedlings, the illumination intensity is 200-.
The dark color in the step (1) is dark blue.
The carbon source in the step (2) is sucrose and cassava powder in a weight ratio of 1: 1.
The seedling releasing in the step (3) is as follows: the seedling density is 400 tails per cubic water body in 300-fold, after the sea horse body is 3 cm high, the small individual sea horse is picked out for additional culture, and the density is gradually reduced to 200 tails per cubic water body in 150-fold until the sea horse is cultured.
The illumination control in the step (3) is as follows: the maximum day time is not more than 700lux, the morning and the evening are not less than 200lux, the illumination time per day is not less than 14 hours, and the illumination time of the light intensity of 400-700lux is not less than 10 hours.
And (3) controlling the water temperature to be 24-30 ℃ and the salinity to be not lower than 15 per thousand during the breeding period of the hippocampus grey sea seedlings.
And (3) feeding the seedlings of the hippocampus Grosvenorii in the culture period of the seedlings of the Hippocampus Grosvenorii in the step (3) with feeding density of 3-5/ml for 2 times every day, wherein the feeding is performed once at 08:00 and once at 15:00 respectively.
And (4) sucking off dead baits and feces during the culture in the step (3), replacing water is not needed, and water is supplemented after sucking and discharging sewage.
In the step (3), the culture container (such as a culture pond) is not changed during the culture period, and the seedlings are released until the seedlings are cultured.
And (4) carrying out Hippocampus Grosvenorii fry breeding in the step (3) under the condition of being provided with a fry breeding system and a breeding environment.
The seedling culture system consists of a culture pond, a water inlet and drainage system, an inflation system and a temperature control system.
The culture pond is 2-12m2The water depth is 60 cm.
According to the method, by utilizing the biological floc principle, the wall of the culture container is brushed into a dark color and the illumination is controlled at the same time, the combination of the three can improve the culture survival rate of the Hippocampus erectus seedlings in summer and reduce the enteritis of the Hippocampus erectus seedlings in the summer culture period, wherein the brushing of the wall of the culture container into the dark color and the illumination control are beneficial for the Hippocampus erectus seedlings to enter a water body.
Advantageous effects
The invention has the outstanding characteristics of reducing the disease incidence rate, particularly the enteritis, reducing the labor intensity, keeping the water quality stable and saving the culture water by utilizing the biological floc principle.
The invention can not only improve the culture effect of normal seedlings, but also has particularly obvious effect on improving the culture effect of low-quality seedlings, can reduce the incidence rate of enteritis to below 20 percent, can not spread even if suffering from enteritis, can gradually recover most diseased hippocampus, can ensure that the survival rate of the high-quality seedlings in summer reaches above 60 percent, and the survival rate of the low-quality seedlings reaches above 40 percent, and greatly improves the culture benefit.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Example 1
First, farming systems
The Hippocampus grey sea horse seedling culture system comprises culture pond, water inlet and outlet system, inflation system and temperature control system, and the breed bucket is the glass steel material, and the inner wall is dark blue, and the reflectance ratio is 18%, and 5 tons of staving volume, water depth 60 centimetres, actual aquaculture water 3 tons. The residual bait and excrement can be gradually concentrated in the middle of the barrel by rotating water and then sucked off.
Second, culture environment
The Hippocampus erectus seedling culture is carried out at the water temperature of 27-30 ℃, the salinity of culture water is 30 per thousand, the daily illumination is 200-.
Feeding bait
Feeding the seedlings to copepods with the feeding density of 3-5/ml twice a day at a rate of 08:00 and 15:00 respectively.
Fourthly, seedling releasing and breeding management
Seedling releasing: the seedling density is increased to 400 tails per cubic water body, after the sea horse body is 3 cm high, the small individual sea horse is picked out for additional culture, and the density is gradually reduced to 150 tails per cubic water body until the sea horse is cultured;
carbon source: half of each of the sucrose and the cassava flour, 6 g of EM (effective microorganisms) is added into each ton of water, and 6 ml of EM is added simultaneously.
Cultivation management: during seedling releasing, simultaneously adding a carbon source and EM (effective microorganisms) bacteria, continuously adding for 7 days after seedling releasing, and adding every other day; when seedlings are put, the water level is only 30 cm, and then water is supplemented every day until the water level is gradually increased to 60 cm. During the culture period, dead bait and excrement are sucked, water does not need to be changed, and water is supplemented after the water is sucked and discharged.
The seedlings are raised to 10 cm high, the survival rate of high-quality seedlings is 64 percent, and the survival rate of low-quality seedlings is 41 percent; the incidence of enteritis is lower than 20%.
Comparative example 1
The experiment was carried out by the conventional method, with only bacteria and no carbon source, and the other conditions were completely equivalent to the flocculation group (example 1). The seedlings are raised to 10 cm high, the survival rate of high-quality seedlings is 48 percent, and the survival rate of low-quality seedlings is 19 percent; the incidence rate of enteritis is about 40 percent.
While the embodiments of the present invention have been described in detail, the present invention is not limited to the embodiments, and those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present invention, and such equivalent modifications or substitutions are intended to be included within the scope of the present invention as defined by the appended claims.

Claims (7)

1. A large-scale gray hippocampus culture method based on a biological floc principle comprises the following steps:
(1) brushing the wall of the culture container into a dark color, wherein the light reflectivity of the dark color is 15-20%;
(2) when the young Hippocampus erectus is released, simultaneously adding carbon source and EM (effective microorganisms) according to the proportion of 5-6 g/ton and 6-7 ml/ton respectively, wherein the water level is 30-35 cm, and then supplementing new water every day until the water level is gradually increased to 60-70 cm; continuously adding a carbon source and EM (effective microorganisms) for 6-7 days after seedling placement, and adding every other day;
(3) the illumination is controlled during the breeding period of the hippocampus griseus seedlings, the illumination intensity is 200-.
2. The farming method of claim 1 wherein in step (1) the dark color is dark blue.
3. The cultivation method according to claim 1, wherein the carbon source in the step (2) is sucrose and tapioca in a weight ratio of 1: 1.
4. The culture method according to claim 1, wherein the seedling releasing in the step (2) is as follows: the seedling density is 400 tails per cubic water body in 300-fold, after the sea horse body is 3 cm high, the small individual sea horse is picked out for additional culture, and the density is gradually reduced to 200 tails per cubic water body in 150-fold until the sea horse is cultured.
5. The cultivation method according to claim 1, wherein the control of the illumination in the step (3) is: the maximum day time is not more than 700lux, the morning and the evening are not less than 200lux, the illumination time per day is not less than 14 hours, and the illumination time of the light intensity of 400-700lux is not less than 10 hours.
6. The culture method of claim 1, wherein in the step (3), the water temperature is controlled to be 24-30 ℃ and the salinity is not lower than 15 per thousand during the culture of the Hippocampus Grey sea seedlings.
7. The culture method of claim 1, wherein in the step (3), the seedlings are fed with copepods at a density of 3-5/ml 2 times per day, once at 08:00 and once at 15:00 respectively during the culture period of Hippocampus griseus seedlings.
CN202010498650.9A 2020-06-04 2020-06-04 Large-scale Hippocampus grey sea horse breeding method based on biological floc principle Active CN111631171B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010498650.9A CN111631171B (en) 2020-06-04 2020-06-04 Large-scale Hippocampus grey sea horse breeding method based on biological floc principle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010498650.9A CN111631171B (en) 2020-06-04 2020-06-04 Large-scale Hippocampus grey sea horse breeding method based on biological floc principle

Publications (2)

Publication Number Publication Date
CN111631171A CN111631171A (en) 2020-09-08
CN111631171B true CN111631171B (en) 2022-01-11

Family

ID=72323831

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010498650.9A Active CN111631171B (en) 2020-06-04 2020-06-04 Large-scale Hippocampus grey sea horse breeding method based on biological floc principle

Country Status (1)

Country Link
CN (1) CN111631171B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112352710A (en) * 2020-10-10 2021-02-12 中国水产科学研究院东海水产研究所 Large-scale Hippocampus japonicus breeding method in high-temperature season

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170056873A (en) * 2015-11-16 2017-05-24 주식회사 네오엔비즈 Feed including organic compounds in biofloc and method for production thereof
CN110226534A (en) * 2019-06-05 2019-09-13 中国水产科学研究院东海水产研究所 A kind of extensive grey hippocampus low-quality seedling cultural method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170056873A (en) * 2015-11-16 2017-05-24 주식회사 네오엔비즈 Feed including organic compounds in biofloc and method for production thereof
CN110226534A (en) * 2019-06-05 2019-09-13 中国水产科学研究院东海水产研究所 A kind of extensive grey hippocampus low-quality seedling cultural method

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
不同放苗密度凡纳滨对虾生物絮团养殖的环境和产出效应;张许光等;《渔业科学进展》;20130615;第34卷(第03期);第111-119页 *
南美白对虾仿生态养殖研究;陈庆荣;《南方农机》;20170628;第48卷(第12期);第83页 *
圆口铜鱼子二代苗种的早期培育;鲁雪报;《科学养鱼》;20160310(第3期);第5-7页 *
大海马幼苗人工养殖条件的研究;戴广谱;《渔业科学进展》;20110815;第32卷(第4期);第62-66页 *
生物絮团在凡纳滨对虾封闭养殖试验中的形成条件及作用效果;邓应能等;《渔业科学进展》;20120415;第33卷(第02期);第69-75页 *
生物絮团技术在水产养殖中的应用研究;张怖青等;《渔业现代化》;20161220;第43卷(第06期);第33-38页 *

Also Published As

Publication number Publication date
CN111631171A (en) 2020-09-08

Similar Documents

Publication Publication Date Title
CN103828748B (en) Pond greenhouse Penaeus Vannmei, the method for three batches of alternate cultures of Macrobrachium rosenbergii
CN103444600B (en) Two-stage efficient rearing method for freshwater shrimp fry
CN103404453B (en) Freshwater shrimp and river crab double main farming method
CN102893937A (en) Efficient carp farming method by using free-of-water changing pond
AU2020103666A4 (en) A preparation method of processing Penaeus vannamei, Carassius auratus and Hypophthalmichthys molitrix
CN105766708A (en) Litopenaeus vannamei relay breeding mode
CN106386611A (en) High-yield method for breeding freshwater shrimps in greenhouse in winter
CN106942226A (en) Promote pasture and water and beneficial algae growing plants growth regulator in shrimp, the crab pool
CN104521815A (en) Technical method for breeding freshwater shrimps in paddy field
CN110226534B (en) Large-scale low-quality hippocampus japonicus fry breeding method
CN106417122A (en) Cultivating method for increasing high-quality freshwater shrimp seed output
CN108703094A (en) A kind of method for breeding of double season freshwater shrimps
CN111631171B (en) Large-scale Hippocampus grey sea horse breeding method based on biological floc principle
CN104663530A (en) Technical method for culturing freshwater crayfish in pond
CN104839079B (en) A kind of utilization prawn culturing waste water indoor scale cultivates the method and facility of Duo Lin Xi parent fishs
CN104705233B (en) A kind of method that four noses palpus Cyprinus carpio cultivating pools raise together with Penaeus vannamei
CN103416335A (en) Method for breeding freshwater shrimp parents by utilizing river crab culture pond
CN107683804A (en) A kind of technical method of pond culture freshwater lobster
CN111771773A (en) Domesticating method suitable for weever in central and north regions
CN111248117A (en) Breeding method of acrossocheilus fasciatus
CN109006605B (en) Freshwater ecological breeding method for penaeus vannamei boone
CN104509457A (en) Method for breeding fish through biogas manure
CN111134050A (en) Grouper and siganus guttatus circulating water mixed culture method
CN113632751B (en) Jellyfish fry production method based on podocyst reproduction
CN112425535A (en) Method for accelerating early fry feeding habit conversion of takifugu rubripes

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant