CN111607003B - 一种SARS-CoV-2 N/S1(RBD)重组蛋白及制备方法和应用 - Google Patents
一种SARS-CoV-2 N/S1(RBD)重组蛋白及制备方法和应用 Download PDFInfo
- Publication number
- CN111607003B CN111607003B CN202010438729.2A CN202010438729A CN111607003B CN 111607003 B CN111607003 B CN 111607003B CN 202010438729 A CN202010438729 A CN 202010438729A CN 111607003 B CN111607003 B CN 111607003B
- Authority
- CN
- China
- Prior art keywords
- sars
- cov
- protein
- rbd
- recombinant protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/20011—Coronaviridae
- C12N2770/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/20011—Coronaviridae
- C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010438729.2A CN111607003B (zh) | 2020-05-21 | 2020-05-21 | 一种SARS-CoV-2 N/S1(RBD)重组蛋白及制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010438729.2A CN111607003B (zh) | 2020-05-21 | 2020-05-21 | 一种SARS-CoV-2 N/S1(RBD)重组蛋白及制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111607003A CN111607003A (zh) | 2020-09-01 |
CN111607003B true CN111607003B (zh) | 2022-08-23 |
Family
ID=72201654
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010438729.2A Active CN111607003B (zh) | 2020-05-21 | 2020-05-21 | 一种SARS-CoV-2 N/S1(RBD)重组蛋白及制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111607003B (zh) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021045836A1 (en) | 2020-04-02 | 2021-03-11 | Regeneron Pharmaceuticals, Inc. | Anti-sars-cov-2-spike glycoprotein antibodies and antigen-binding fragments |
CN112048007B (zh) * | 2020-09-11 | 2022-07-12 | 北京美康基免生物科技有限公司 | 一种通用型新型冠状病毒疫苗及其制备方法 |
CN112168958B (zh) * | 2020-09-21 | 2022-05-06 | 上海交通大学 | 基于慢病毒外壳修饰和mRNA递送的SARS-CoV-2疫苗及其制备方法 |
CN112666348A (zh) * | 2020-10-27 | 2021-04-16 | 山西高等创新研究院 | 新型冠状病毒SARS-CoV-2的检测蛋白组及应用 |
EP4029569A1 (en) * | 2020-12-02 | 2022-07-20 | Mammedov, Tarlan | Engineering, production and characterization of plant produced nucleocapsid and spike structural proteins of sars-cov-2 as vaccine candidates against covid-19 |
CN112229994B (zh) * | 2020-12-10 | 2021-03-16 | 丹娜(天津)生物科技股份有限公司 | 一种基于磁微粒化学发光的新型冠状病毒抗体检测试剂盒 |
CN112321688B (zh) * | 2021-01-06 | 2021-03-26 | 广东华南疫苗股份有限公司 | 稳定的冠状病毒重组蛋白二聚体及其表达载体 |
CN116940589A (zh) * | 2021-01-29 | 2023-10-24 | 上海锦斯生物技术有限公司 | 重组sars-cov-2疫苗 |
CN113024641B (zh) * | 2021-03-11 | 2022-05-17 | 广东省农业科学院动物卫生研究所 | 新型冠状病毒的重组s蛋白及其制备方法和应用 |
CN113186204B (zh) * | 2021-06-11 | 2021-10-15 | 北京华芢生物技术有限公司 | 新型冠状病毒巴西株p.1突变株rbd的基因及其应用 |
CN113502294B (zh) * | 2021-07-15 | 2023-03-17 | 四川大学华西医院 | 新型冠状病毒rbd蛋白的制备方法以及新型冠状病毒疫苗 |
WO2023017536A1 (en) * | 2021-08-12 | 2023-02-16 | Reliance Life Sciences Pvt. Ltd. | Immunogenic compositions for sars- cov-2 |
CN113480619B (zh) * | 2021-08-26 | 2023-05-09 | 深圳市亚辉龙生物科技股份有限公司 | 多肽及其在新型冠状病毒检测中的应用 |
CN113755421B (zh) * | 2021-09-28 | 2024-04-12 | 梦芊细胞因子有限公司 | 一种用于covid-19的口服性疫苗及抗体加强剂 |
WO2023081905A2 (en) * | 2021-11-08 | 2023-05-11 | Georgia Tech Research Corporation | Detection platform for unlabeled oligonucleotides |
BR102022000733A8 (pt) * | 2022-01-14 | 2023-04-11 | Univ Minas Gerais | Processo de produção de proteína quimérica, proteína quimérica, gene, composição imunogênica, e usos |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004110483A1 (en) * | 2003-05-31 | 2004-12-23 | Yiyou Chen | Method and composition of a novel vaccine design for the prevention and treatment of sars |
US7320857B2 (en) * | 2003-06-18 | 2008-01-22 | Chinese National Human Genome Center At Shanghai | Characterization of the earliest stages of the severe acute respiratory syndrome (SARS) virus and uses thereof |
CN100386343C (zh) * | 2003-07-03 | 2008-05-07 | 李越希 | Sars病毒s蛋白与n蛋白的融合蛋白及其制备、应用 |
GB0911794D0 (en) * | 2009-07-07 | 2009-08-19 | Animal Health Inst | Chimaeric protein |
CN111024954A (zh) * | 2020-03-09 | 2020-04-17 | 深圳市易瑞生物技术股份有限公司 | 联合检测covid-19抗原和抗体的胶体金免疫层析装置及其使用法 |
CN111089962B (zh) * | 2020-03-25 | 2020-07-17 | 中山生物工程有限公司 | 联合检测新型冠状病毒IgM/IgG抗体的胶体金试剂盒及制备方法 |
-
2020
- 2020-05-21 CN CN202010438729.2A patent/CN111607003B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN111607003A (zh) | 2020-09-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111607003B (zh) | 一种SARS-CoV-2 N/S1(RBD)重组蛋白及制备方法和应用 | |
JP2012531198A (ja) | Crm197及びその誘導体の産生のための人工遺伝子の細菌発現 | |
CN106939042B (zh) | 一种猪α干扰素及其应用 | |
CN110964096B (zh) | 一种重组人源c反应蛋白的制备方法 | |
CN110592057B (zh) | 嵌合裂解酶ILTphg和编码此酶的多核苷酸 | |
CN114657113A (zh) | 一种表达全能核酸酶的重组菌及其应用 | |
CN111777683A (zh) | 神经坏死病毒MCP与鮰爱德华氏菌ompN1的融合蛋白及其制备方法 | |
WO2000059537A1 (en) | System for efficient secretion of recombinant proteins | |
WO2018136572A1 (en) | Expression construct and method for producing proteins of interest | |
JP2010512168A (ja) | 組換えヒトアルギナーゼiについての改善された発現系 | |
CN116355887A (zh) | 一种链球菌前噬菌体裂解酶lys733及其应用 | |
CN107746432B (zh) | 一种Aβ42修饰蛋白及其表达与纯化方法 | |
CN116589591A (zh) | MBP标签、Spy标签或MBP-Spy串联标签在辅助重组蛋白表达中的应用 | |
CN108300725B (zh) | 可溶性单链抗体超抗原融合基因及蛋白和其制备与应用 | |
CN111018955B (zh) | 一种抑制病毒基因组rna复制的多肽 | |
CN112316130B (zh) | 一种SARS-CoV2粘膜免疫疫苗及其应用 | |
US9096880B2 (en) | Solubility enhancing peptide and use thereof | |
KR20130141001A (ko) | 목적 단백질의 분리 및 정제를 위한 신규한 벡터 시스템 | |
CN113025599A (zh) | 一种重组溶组织梭菌i型胶原酶及其制备方法和应用 | |
Wang et al. | Preparation of a peptide vaccine against GnRH by a bioprocess system based on asparaginase | |
CN105017399A (zh) | 日本血吸虫SjPPase重组抗原蛋白及其制备方法和用途 | |
CN112898407B (zh) | 重组驼源血清白蛋白的制备方法 | |
CN112941058B (zh) | 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用 | |
CN116640755B (zh) | 一种链球菌前噬菌体裂解酶lys1519及其应用 | |
CN113683707B (zh) | 一种抗原融合蛋白及其编码基因和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 201802 room j5061, floor 1, building 1, No. 1185, Huyi highway, Jiading District, Shanghai Applicant after: Shanghai Baiying Biotechnology Co.,Ltd. Address before: 225300 west side of 4th floor, No. G25, building 0005, east side of Tai Road and north side of Xinyang Road, China medicine Chengkou, Taizhou City, Jiangsu Province Applicant before: Hundred English bio tech Ltd. of Taizhou City |
|
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 201318 4th floor, 416 Zhoushi Road, Pudong New Area, Shanghai Applicant after: Shanghai Baiying Biotechnology Co.,Ltd. Address before: 201802 room j5061, floor 1, building 1, No. 1185, Huyi highway, Jiading District, Shanghai Applicant before: Shanghai Baiying Biotechnology Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: Room 101, 106, 201, 301, 401, Building 1, No. 1-9, Lane 99, Shenmei Road, Zhoupu Town, Pudong New Area, Shanghai, 201200 Patentee after: Shanghai Baiying Biotechnology Co.,Ltd. Address before: 201318 4th floor, 416 Zhoushi Road, Pudong New Area, Shanghai Patentee before: Shanghai Baiying Biotechnology Co.,Ltd. |