CN111587687A - Plant grafting cultivation method - Google Patents
Plant grafting cultivation method Download PDFInfo
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- CN111587687A CN111587687A CN202010569375.5A CN202010569375A CN111587687A CN 111587687 A CN111587687 A CN 111587687A CN 202010569375 A CN202010569375 A CN 202010569375A CN 111587687 A CN111587687 A CN 111587687A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
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Abstract
The invention discloses a plant grafting cultivation method, which comprises the following steps: (1) soaking the root end of the dug and selected stock in a soaking agent for bare root cultivation of plants until the top end of the stock has a moist feeling; (2) taking out the soaked rootstock, coating a healing agent for the wound surface of a plant on the port of the rootstock, and planting the rootstock to soil to be used as a female pile; (3) cutting bud pieces on the selected tender buds, removing xylem on the inner sides of the bud pieces, reserving cambium layers on the inner sides of the bud pieces, preparing scion buds, and soaking the scion buds in the scion bud soaking agent for later use; (4) cutting a bud grafting interface on the stock, reserving a cambium on the stock at the bud grafting interface, cleaning the soaked scion bud, inserting the scion bud into the bud grafting interface, attaching the cambium of the scion bud and the cambium of the stock, and binding and fastening the cambium of the scion bud and the cambium of the stock by using a film to complete grafting. The method for grafting and cultivating the plants can obviously improve the germination rate and the survival rate, and has no black bud, and the new buds grow strongly and are viable.
Description
Technical Field
The invention belongs to the technical field of seedling cultivation, and particularly relates to a plant grafting cultivation method.
Background
Grafting, one of the vegetative reproduction in vegetative reproduction. During grafting, the scion and the cambium of the stock are tightly combined to ensure the survival of the scion. The grafted branch or bud is called scion; the grafted plant is called stock or table wood. When scion is carried out, a seedling with 2 to 4 buds is generally selected, the grafted seedling becomes the upper part or the top of a plant body, and a stock becomes the root part of the plant body after grafting. The grafting method is generally used for plant cultivation, but the existing plant grafting technology has certain crude performance, and particularly in the aspect of detail control, when the grafting technology is applied to landscape plants, the grafting technology is easy to cause the problems of low survival rate of the grafted plants, short survival time, low ornamental value and the like due to the phenomena of black buds growing after bud grafting, stock end cracking and the like.
The flos Rosae chinensis is also named as herba Catharanthi rosei and flos Rhododendri Simsii, and is a plant of Rosa of Rosaceae. The China rose has beautiful flower color, and the color is colorful and even gorgeous; the flower is opened according to the month, the flowering period is longer, flower beds and flower beds are planted or planted in the garden in pieces, flower basketry, bonsai and the like are manufactured, cultivation is carried out all over the country, and the flower basketry is an important ornamental flower in the world and is one of ten major flowers in China. In addition, the tree-like Chinese rose, also called Chinese rose tree and rose tree, is a new type of Chinese rose produced by a vertical trunk through gardening means (measures such as cuttage, root cultivation, stem cultivation, grafting, pruning and shaping).
At present, the Chinese rose propagation method mainly comprises cutting propagation, grafting propagation or in vitro tissue culture propagation, the in vitro tissue culture propagation needs facilities and high investment, and the cutting propagation and the grafting propagation are used as main propagation modes in the production at present. Cutting propagation refers to the regeneration of roots and buds to develop into new individuals after the vegetative organs of plants are separated from the parent bodies. The propagation mode of the nursery stock has great influence on the yield and the quality of the Chinese rose cut flower. The ornamental characteristics of the Chinese roses can be reserved by cutting propagation, the propagation quantity is enlarged, the operation is simple and convenient, the survival rate is high, particularly, the cutting is performed in autumn, the nutrients of the cutting are sufficient, the disease is not easily infected, the preservation rate of the cutting can be improved, and the growth quantity is advanced by half a year compared with that of the cutting performed in spring of the next year. Research has shown that the rooting rate of cuttage is not only influenced by cutting and hormone, but also the operation of cuttage and management after cuttage are particularly important. The grafting belongs to the category of vegetative propagation, and two plant parts are combined into a whole and grow continuously like a plant. Some Chinese roses have few varieties and vivid colors, while self-rooted seedlings have weak root systems and extremely slow growth. The current common Chinese rose grafting methods include scion bud grafting, T-shaped bud grafting, large-opening bud grafting and rootstock-free grafting.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a plant grafting cultivation method.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the plant grafting cultivation method comprises the following steps:
(1) soaking the root end of the dug and selected stock in a soaking agent for bare root cultivation of plants until the top end of the stock has a moist feeling;
(2) taking out the soaked rootstock, coating a healing agent for wound surfaces of plants on the port of the rootstock, planting the rootstock to soil, and taking the rootstock as the rootstock to be grafted; preferably, the rootstock subjected to grafting is a rootstock with semi-lignified branches which is planted in soil and grows for about five months and then grows to be about one meter long;
(3) cutting bud pieces on the selected tender buds, removing xylem on the inner sides of the bud pieces, reserving cambium layers on the inner sides of the bud pieces, preparing scion buds, and soaking the scion buds in the scion bud soaking agent for later use;
(4) and (3) cutting a bud grafting interface slightly larger than the scion bud from the stock by tearing the bark, reserving a cambium on the stock at the bud grafting interface, cleaning the soaked scion bud, inserting the scion bud into the bud grafting interface, attaching the cambium of the scion bud to the cambium of the stock, and binding and fastening the cambium of the scion bud by using a film to complete grafting.
Preferably, every 1000kg of the soaking agent for cultivating the plant bare roots in the step (1) contains 150-250g of chlorobromoisocyanuric acid, 550mL of orange peel essential oil synergistic additive, 350mL of chlorpyrifos missible oil and the balance of water. More preferably, every 1000kg of the soaking agent for cultivating the plant bare roots in the step (1) contains 200g of chlorobromoisocyanuric acid, 500mL of orange peel essential oil synergistic auxiliary agent, 300mL of chlorpyrifos emulsifiable concentrate and the balance of water.
Preferably, the chlorobromoisocyanuric acid is 50% chlorobromoisocyanuric acid, and the chlorpyrifos emulsifiable concentrate is 40% chlorpyrifos emulsifiable concentrate. Wherein the mass percentage concentration of the main component in the composition is a general expression mode in the field.
Preferably, the healing agent for plant wound surface in step (2) is prepared by the following method: adding 150-250mL of methylthio-tebuconazole suspending agent into every 2-3kg of tung oil to obtain the product. More preferably, 200mL of the methiazole suspending agent is added per 2.4kg of tung oil.
Preferably, the methylthio-tebuconazole suspending agent is 48% methylthio-tebuconazole suspending agent. Wherein the mass percentage concentration of the main component in the composition is a general expression mode in the field.
Preferably, the healing agent for plant wound surfaces further contains polyamine, and the mass ratio of the polyamine to the tung oil in the healing agent for plant wound surfaces is (3-5) g: (4-5) kg. More preferably, the mass ratio of the polyamine to the tung oil in the healing agent for the plant wound surface is 4 g: 4.6 kg.
Preferably, every 500mL of the scion bud soaking agent in the step (3) contains 1.5-2.5g of chlorobromoisocyanuric acid, 1.5-2.5g of glucose, 121.5-2.5 g of vitamin B, 0.5-1.5g of polyamine and the balance of water. More preferably, every 500mL of the scion bud soaking agent in the step (3) contains 2g of chlorobromoisocyanuric acid, 2g of glucose, vitamin B122 g, 1g of polyamine and the balance of water. More preferably, the chlorobromoisocyanuric acid is 50% chlorobromoisocyanuric acid. Wherein the mass percentage concentration of the main component in the composition is a general expression mode in the field.
Preferably, the rootstock digging in the step (1) and the rootstock planting in the step (2) are carried out after autumn and at a temperature of more than 10 ℃; the bud grafting time in the step (4) is any time in one year, but the temperature is required to be more than 5 ℃, and the unused young branches are preserved under the condition of cold and shade.
Preferably, if the budding in step (4) is performed in winter, the budded plants are covered with film bags for heat preservation and moisture preservation.
Preferably, the rootstock is selected from a large flower rose or a long-tipped rose, and the tender bud strip is selected from a rose country angel rose; when the plant grafting cultivation process encounters the wound surface of the plant, the healing agent for the wound surface of the plant in the step (2) can be used, and for example, the healing agent can be smeared at a grafting opening.
The invention is further illustrated below:
according to the invention, after the rootstock is dug, the root of the rootstock is soaked in the soaking agent for bare-root cultivation of plants, and the rootstock is soaked until the top end of the rootstock has a moist feeling, so that the rootstock is activated, and the growth supply capacity of the rootstock is improved. The soaking agent contains chlorobromoisocyanuric acid, orange peel essential oil synergistic auxiliary agent and chlorpyrifos missible oil. 50% of chlorobromoisocyanuric acid can quickly eliminate pathogens of fungi, viruses and bacteria, can obviously resist infection of various diseases and insect pests when no diseases and insect pests occur, and has an ultra-short time (3 seconds) of taking effect in treating the diseases and insect pests of crops, thereby being very efficient; particularly, the method is more effective when crops are mixed with diseases and insect pests or the types of the diseases and the insect pests are not judged clearly, and is a killer for difficult diseases and insect pests; and after being dissolved in water, hypobromous acid and hypochlorous acid can be decomposed, so that bacteria, fungi and viruses can be effectively killed, and the healthy growth of trees can be obviously promoted. The orange peel essential oil synergistic auxiliary agent has extremely strong penetration and diffusion capacity, penetrates through plant epidermis and airway passages, has dual effects of penetration and diffusion, and can inhibit hypha germination by properly reducing the dosage under the same control effect. The chlorpyrifos emulsifiable solution has the triple effects of stomach poisoning, contact killing and fumigating, and has better simulation effect on various pests with chewing type and piercing-sucking type mouthparts. The compatibility is good, and the pesticide composition can be mixed with various insecticides and bactericides and has obvious synergy. The insecticidal spectrum is wide, the insecticidal composition is easy to combine with organic matters in soil, and has a special effect on underground pests such as borers, and the effective period is as long as more than 30 days. The three components are used in a compatible manner, and practice shows that the three components generate synergistic effect and promote each other, so that the soaked stock has strong growth supply capacity, antibacterial and anti-toxic capacity, and can provide reliable guarantee for subsequent grafting as a stock pile.
After the rootstock is soaked, the healing agent is coated on the wound surface of the tree, and the healing agent mainly comprises tung oil and a methidazole suspending agent, and can also be added with polyamine (the effect is better after the polyamine is added). The tung oil belongs to excellent dry vegetable oil, has the advantages of quick drying, good glossiness, strong adhesive force, acid resistance, alkali resistance, heat resistance and corrosion resistance, and can be quickly absorbed by the wound surface of a plant. The tung oil has good bactericidal effect and is widely applied to the medical field. The tebuconazole is a triazole spectrum bactericide, has strong systemic property, high activity and long lasting period, and has high activity on fruit tree rot disease through tests, and can quickly control the development and spread of the rot disease. The polyamine is a multifunctional colorless odorless pure biological preparation, and is a non-hormone product. Contains plant endogenous active ingredients, and is related to plant growth regulation and immunity induction substances. Can induce the plant to generate the synthesis or the improvement of the activity of the anti-injury, anti-disease and anti-adversity components and the plant defense substances, increase the anti-injury, anti-disease and anti-adversity capability of the plant, promote the plant to be healthy and strong and prevent premature senility. The three components are used in a compatible manner, and practice shows that the three components generate synergistic effect and promote each other, so that the coated stock has strong growth stability, the condition of end cracking (opening) is avoided, and reliable guarantee is further provided for subsequent grafting.
When the scion bud is prepared, bud pieces are cut from young shoots collected or stored at present, xylem on the inner sides of the bud pieces is removed, cambium on the inner sides of the bud pieces is reserved (different from the traditional bud grafting mode, the xylem on the inner sides of the bud pieces is required to be reserved in the traditional bud grafting mode in many times), scion buds are prepared, and then the scion buds are soaked in a scion bud soaking agent, wherein the scion bud soaking agent contains chlorobromoisocyanuric acid, glucose, vitamin B12 and polyamine, vitamin B12 can promote rooting, improve resistance and enable leaves to be bright, and after the vitamin B12 is matched with the chlorobromoisocyanuric acid, the glucose and the polyamine, the generation of a black bud phenomenon can be effectively inhibited.
In the invention, a bud grafting interface slightly larger than a scion bud is cut on a stock by tearing the bark, a cambium on the stock at the bud grafting interface is reserved, the soaked scion bud is cleaned and then inserted into the bud grafting interface, the cambium of the scion bud is attached to the cambium of the stock, and then the cambium of the scion bud is bound and fastened by a film to complete grafting. Thus, the cambium of the scion bud can be tightly combined with the cambium of the stock, the cambium can continuously generate new xylem and phloem, the stem or the root is continuously thickened, the cambium is divided towards the central axis direction of the stem to form new xylem cells, the xylem cells are divided outwards to form new phloem cells, and the cambium is one of lateral meristems formed in the cortex by secondary thickening growth of the stem, the root and the like of the plant. The close adhesion of the cambium promotes the growth of scion buds. The cambium and the scion bud cambium on the rootstock are kept, so that the tissues same as the plants can be fused with each other to form a state of 'you in you, you in you' in a very short time to grow into a new connective tissue, if the cambium of the rootstock is cut off and is butted with the xylem of the scion bud, the time for forming the cambium in a long shape is prolonged, and the death of black head infection of the scion bud is easily caused; however, if the xylem of the scion bud is retained, it is the same as the cause of pain in the tissues left in the body for a long time after the bone setting operation in humans in rainy days. And only when the cambium of the rootstock and the scion bud are reserved and butted, the cambium of the rootstock and the scion bud can be quickly fused in a short time under the action of the soaking agent, so that the growth of the bud is promoted, and a solid support is provided for wind resistance in the middle and later stages.
The components of the medicament involved in the invention are all endogenous components of plants, are green and environment-friendly, and have no other stimulation or negative effect on the plants.
By grafting and cultivating the plants by the grafting method, the budding rate after grafting can be obviously improved, the survival rate is improved, black buds do not exist, the growth vigor of new buds is strong and active, the problems of slow growth of grafting root systems, high black bud rate, root stock opening explosion and the like in the prior art are effectively solved, in addition, the callus growth vigor of the root parts of the root stocks coated with the healing agent is good, and the root growing phenomenon exists on the callus.
Detailed Description
Example 1
Grafting and cultivating rose country angel China rose in Yunnan red river region, soaking rootstock end of the large flower perfume China rose dug and selected at the temperature of more than 10 ℃ after autumn in a soaking agent for bare root cultivation of plants until the top end of the rootstock is touched to have moist feeling; taking out the soaked rootstock, coating a healing agent for wound surfaces of plants on the port of the rootstock, planting the rootstock to soil, and taking the rootstock as the rootstock to be grafted; preferably, the rootstock subjected to grafting is a rootstock with semi-lignified branches which is planted in soil and grows for about five months and then grows to be about one meter long; cutting bud slices on young bud strips of the selected rose country-wide Chinese roses, removing xylem on the inner sides of the bud slices, reserving cambiums on the inner sides of the bud slices, preparing scion buds, and soaking the scion buds in the scion bud soaking agent for later use; and (3) cutting a bud grafting interface slightly larger than the scion bud from the stock by tearing the bark, reserving a cambium on the stock at the bud grafting interface, cleaning the soaked scion bud, inserting the scion bud into the bud grafting interface, attaching the cambium of the scion bud to the cambium of the stock, and binding and fastening the cambium of the scion bud by using a film to complete grafting. Wherein, every 1000kg of the soaking agent for cultivating the plant bare roots contains 200g of chlorobromoisocyanuric acid, 500mL of orange peel essential oil synergistic auxiliary agent, 300mL of 40% chlorpyrifos emulsifiable concentrate and the balance of water. The healing agent for the wound surface of the plant is prepared by the following method: adding 200mL of 48% methionyl-tebuconazole suspending agent into every 2.4kg of tung oil, preferably, adding polyamine into the healing agent, wherein the mass ratio of polyamine to the tung oil is 4 g: 4.6 kg. Each 500mL of the scion bud soaking agent contains 2g of chlorobromoisocyanuric acid, 2g of glucose, vitamin B122 g, 1g of polyamine and the balance of water.
Meanwhile, the traditional grafting method (the stock is not soaked and the traditional medicament is used instead of the medicament) is adopted for comparison. The result shows that the grafted Chinese rose sprouts by the method in 10 to 15 days, the black bud rate is 0 percent, the sprouts are regular and strong, the joints are healed and have obvious callus, and the newly-grown sprouts can resist strong wind after two months. The Chinese rose grafted by the traditional method has large-area black buds, the buds are slowly emerged, and the end of the stock has the sign of cracking.
Example 2
Grafting cultivation of rose country-wide angel rose was carried out in the region of red river of Yunnan, in the same manner as in example 1, except that the stock was selected from long-tipped rose, and the result was identical to that of example 1.
Example 3
Grafting cultivation of rose country angel rose is carried out in the Yunnan red river region, the same method as that of the embodiment 1 is adopted, two groups of test groups are added to the control test group besides the traditional method group, the method of the embodiment 1 is also adopted in the added two groups of test groups, but the method of the scion bud soaking agent different from the method of the embodiment 1 is adopted: in the control test group 1, each 500mL of the scion bud soaking agent contains 2g of chlorobromoisocyanuric acid, 2g of glucose and the balance of water; in the control test group 2, each 500mL of the scion bud soaking agent contains 2g of chlorobromoisocyanuric acid, 2g of glucose, vitamin B122 g and the balance of water. The results show that: in the control test group 1, the black bud rate is 6%, and the bud growth after germination is slightly stronger than that of the bud grafted by the traditional method; in the control test group 2, the black bud rate was 3%, and the bud growth after germination was stronger and the germination was uniform than that of the conventional method and the control test group 1. After the test group adopting the method of the example 1 and the scion bud soaking agent is grafted for 15 days, the germination rate reaches 90%, 2-4 buds emerge from 10% of the buds, the buds are full and viable, no black bud exists, and the growth vigor is strong.
Claims (10)
1. A plant grafting cultivation method is characterized by comprising the following steps:
(1) soaking the root end of the dug and selected stock in a soaking agent for bare root cultivation of plants until the top end of the stock has a moist feeling;
(2) taking out the soaked rootstock, coating a healing agent for wound surfaces of plants on the port of the rootstock, planting the rootstock to soil, and taking the rootstock as the rootstock to be grafted;
(3) cutting bud pieces on the selected tender buds, removing xylem on the inner sides of the bud pieces, reserving cambium layers on the inner sides of the bud pieces, preparing scion buds, and soaking the scion buds in the scion bud soaking agent for later use;
(4) and (3) cutting a bud grafting interface slightly larger than the scion bud from the stock by tearing the bark, reserving a cambium on the stock at the bud grafting interface, cleaning the soaked scion bud, inserting the scion bud into the bud grafting interface, attaching the cambium of the scion bud to the cambium of the stock, and binding and fastening the cambium of the scion bud by using a film to complete grafting.
2. The method as claimed in claim 1, wherein the soaking agent for cultivating the plant bare roots in the step (1) contains 150 g of chlorobromoisocyanuric acid, 550mL of orange peel essential oil synergist, 350mL of chlorpyrifos emulsifiable concentrate and the balance of water, wherein the soaking agent comprises 150 g of chlorobromoisocyanuric acid, 550mL of orange peel essential oil synergist, 250mL of chlorpyrifos emulsifiable concentrate and the balance of water.
3. The method of claim 2, wherein said chlorobromoisocyanuric acid is 50% chlorobromoisocyanuric acid and said chlorpyrifos emulsifiable concentrate is 40% chlorpyrifos emulsifiable concentrate.
4. The method of claim 1, wherein the healing agent for plant wound surface of step (2) is formulated as follows: adding 150-250mL of methylthio-tebuconazole suspending agent into every 2-3kg of tung oil to obtain the product.
5. The method of claim 4, wherein said methionyl-tebuconazole suspending agent is 48% methionyl-tebuconazole suspending agent.
6. The method according to claim 4 or 5, wherein the healing agent for plant wound surface further contains adductine, and the mass ratio of the adductine to the tung oil in the healing agent for plant wound surface is (3-5) g: (4-5) kg.
7. The method according to claim 1, wherein the scion bud soaking agent comprises 1.5-2.5g of chlorobromoisocyanuric acid, 1.5-2.5g of glucose, 121.5-2.5 g of vitamin B, 0.5-1.5g of adductor, and the balance of water, per 500mL of the scion bud soaking agent in step (3).
8. The method according to claim 1, wherein the rootstock harvest of step (1) and the rootstock planting of step (2) are performed after autumn and at a temperature below 26 ℃; the bud grafting time in the step (4) is any time in one year, but the temperature is required to be more than 5 ℃, and the unused young branches are preserved under the condition of cold and shade.
9. The method according to claim 8, wherein if the budding in step (4) is performed in winter, the budded plants are heat-preserved and moisturized by covering with a film bag.
10. The method of claim 1, wherein the rootstock is selected from a large flower rose or a long steeple rose, and the tender shoot is selected from a rose of the national Angel rose; the healing agent for the wound surface of the plant in the step (2) can be used when the wound surface of the plant is met in the grafting and cultivating process of the plant.
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