CN111557304A

CN111557304A - Application of alkaloid atropine in relieving low-temperature freezing injury stress of plant seedlings

Info

Publication number: CN111557304A
Application number: CN202010446782.7A
Authority: CN
Inventors: 吕艳; 黄涌; 罗丹; 张学昆; 程勇; 邹锡玲; 陆光远
Original assignee: Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
Current assignee: Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
Priority date: 2020-05-22
Filing date: 2020-05-22
Publication date: 2020-08-21
Anticipated expiration: 2040-05-22
Also published as: CN111557304B

Abstract

The invention discloses application of alkaloid atropine in relieving low-temperature freezing injury stress of plants, wherein the cold resistance of the plants can be improved by exogenous alkaloid atropine when cold injury occurs. Under the condition of low temperature stress, the root soaking treatment of the rape seedlings can be carried out by setting the concentration of 50-150 nmol/plant alkaloid atropine aqueous solution, so that the cold damage stress of the rape is relieved; under the condition of low-temperature stress, the concentration of the atropine aqueous solution of 10-30 nmol/plant alkaloid can be set for irrigating arabidopsis seedlings, and the arabidopsis seedling irrigation water-soluble fertilizer has a relieving effect on the freezing stress of arabidopsis. The invention adopts the treatment of the alkaloid atropine to relieve the damage of low temperature to the seedling stage of plants, and opens up a new way for ensuring the safe overwintering of rape seedlings.

Description

Application of alkaloid atropine in relieving low-temperature freezing injury stress of plant seedlings

Technical Field

The invention belongs to the field of agricultural plant production, and particularly relates to application of an alkaloid atropine in relieving low-temperature freezing injury stress of plants.

Background

The rape is the fifth crop of China succeeding rice, wheat, corn and soybean, and bears the important role of domestic plant oil supply, so the development of the rape industry is an important component of agricultural production in China and has important significance on national oil supply safety.

In the Yangtze river basin of the main winter rape producing area, the simplified cultivation and mechanized planting are promoted, and the direct seeding area of the rape is enlarged year by year. However, the direct seeding planting mode of rape cannot be completely adapted to the planting mode of rice-oil rotation in Yangtze river basin, and is often contradictory to crops such as rice for crops, so that the field temperature and light resources in the process of seed germination and seedling growth are obviously reduced compared with those in the process of sowing in proper time, and rape seedlings are weak and have poor resistance. Because the temperature is continuously low in winter, the rape is subjected to delayed low-temperature cold damage, the vegetative growth is slow, the biomass accumulation is low, and the yield of the rape is severely limited. Therefore, the low-temperature stress is one of the important factors for restricting the rape yield in China under the delayed sowing condition.

In order to adapt to different environmental changes, plants synthesize various metabolites or secondary metabolites in the long-term evolution process, and are used for resisting biotic or abiotic adversity stress. The tropane alkaloid is a secondary metabolite mainly distributed in solanaceae plants, is mainly synthesized and stored in roots and can also be transported to the overground part for storage, and is an anticholinergic drug widely applied clinically. Until now, it is not clear whether exogenous tropane alkaloids, as protective agents, have a promoting effect on abiotic resistance of oilseed rape and arabidopsis thaliana. In the research, exogenous alkaloid atropine is adopted to relieve the damage of low temperature to plant seedlings, and a novel protective agent is provided for the plants to resist abiotic stress.

Disclosure of Invention

The invention aims to provide the application of the alkaloid atropine in relieving the freezing injury stress of plant seedlings, and the alkaloid atropine is adopted to relieve the damage of low temperature to the plant seedling stage, so that a new way is opened up for ensuring the safe overwintering of the plant (especially rape) seedlings.

The structural formula of the alkaloid atropine is as follows:

in order to achieve the purpose, the invention adopts the following technical scheme:

the application of the alkaloid atropine in relieving the freeze injury stress of plant seedlings, further, the plant seedlings comprise seedlings of rape and arabidopsis, and the specific method comprises the following steps:

root soaking rape seedlings by using 50-150 nmol/plant of alkaloid atropine aqueous solution;

the arabidopsis seedlings are irrigated by 10-30 nmol/plant of alkaloid atropine aqueous solution.

In a specific example of the invention, rape seedlings were immersed in liquids containing different concentrations (50, 100, 150 nmol/strain) of the alkaloid atropine, stressed at 0 ℃ for 1 day at low temperature, then transferred to normal growth conditions for 3 days of recovery, 7-10 plants each, 3 biological replicates. The results show that the control group rape seedlings show obvious leaf wilting phenomenon, only a small part of the leaves of the treated group rape seedlings show slight shrinkage phenomenon, the leaf wilting number of the control group is obviously higher than that of the treated group rape leaves, but the survival rate of the control group rape seedlings is not different. The atropine aqueous solution with the concentration range of 50-150 nmol/strain is shown to have the effect of relieving the freezing injury of the rapes.

In a specific example of the present invention, Arabidopsis thaliana seedlings were irrigated with aqueous solutions of (10, 30 nmol/strain) the alkaloid atropine at different concentrations, then the seedlings were placed in an incubator at-4 ℃ for 4 hours, then transferred to normal growth conditions for 3 days of recovery, and the survival rate was counted. The results show that the leaves of the control group are withered and yellow or die, the freeze injury symptoms of the Arabidopsis plants of the treatment group are obviously relieved, the survival rate is obviously higher than that of the control group, wherein the effect is the best in 10 nmol/plant treatment group, and the survival rate is 95.0%.

Compared with the prior art, the invention has the following advantages and effects:

the invention uses the alkaloid atropine in relieving the freezing damage stress of arabidopsis thaliana and rape, has simple and easy application method and strong operability, and can obviously improve the freezing resistance of arabidopsis thaliana and rape seedlings.

Drawings

FIG. 1 is a diagram of the growth of rape seedlings after low temperature stress and treatment with different concentrations of the alkaloid atropine.

FIG. 2 shows the growth of Arabidopsis seedlings after low temperature stress and treatment with atropine of different concentrations of alkaloid.

Detailed Description

Example 1

The application of the alkaloid atropine in relieving the freeze injury of rape seedlings comprises the following specific steps:

1. attaching a layer of nylon net on a rectangular plastic box, tightening the nylon net by using a rubber band, placing the nylon net in a water culture box filled with 1/4 Hoagland culture solution, contacting the nylon net with the liquid surface, selecting full seeds, arranging the seeds on the nylon net, placing the nylon net in a culture room at 25 ℃, culturing for 1 week (16-hour light/8-hour dark), then selecting rape seedlings with consistent growth vigor, clamping the rape seedlings into a plastic plate with holes, and soaking roots into the culture solution for continuous culture for one week;

2. an aqueous solution containing the alkaloid atropine, purchased from Moxishi Biotech, Inc., Shanghai, under the designation M18261, with a purity of 99%, was prepared according to Table 1;

3. the roots of the seedlings were immersed in liquids containing the alkaloid atropine at different concentrations, stressed at low temperature of 0 ℃ for 1 day, then transferred to normal growth conditions for 3 days of recovery, each treatment of 7-10 plants, 3 biological replicates. The results show that after the rape seedlings are subjected to cold damage treatment, compared with the treatment groups 1, 2 and 3, the leaves of the control group have obvious leaf wilting and a large number of leaf wilting, while the leaves of the seedlings of the treatment groups 1 and 2 do not shrink, only a small part of the seedlings of the treatment group 3 slightly shrink, and the leaf wilting number of the control group is higher than that of the control group (table 2), which indicates that the atropine aqueous solution with the concentration range of 50-150 nmol/atropine can relieve the cold damage of the rape to different degrees, wherein the relieving effect of 50 nmol/atropine is most obvious.

TABLE 1 treatment concentration of the alkaloid atropine

TABLE 2 statistic table of wilting number of leaves of rape seedlings

Example 2

The application of the alkaloid atropine in relieving the low-temperature freezing stress of arabidopsis thaliana is specifically as follows:

1. sterilizing the harvested Arabidopsis seeds, and then vernalizing the seeds in a refrigerator at 4 ℃ for 3 days;

2. sowing Arabidopsis seeds in 1/2MS solid culture medium, placing in a light incubator, transferring to a culture medium (vermiculite: nutrient soil mixed at a volume ratio of 1: 1) after germination, and culturing for 1 month;

3. preparing a control group, a treatment group 1 and a treatment group 2 according to the table 3, selecting seedlings with the same growth vigor, irrigating alkaloid aqueous solution with different concentrations at the root position of each seedling for one time, culturing overnight, treating each seedling for 3 bowls, and repeating the biology for 3 times;

4. the seedlings were placed in an incubator at-4 ℃ for 4 hours and then transferred to normal growth conditions for 3 days of recovery.

The results show that the control arabidopsis shows a relatively obvious wilting phenomenon after cold damage treatment, after 3 days of recovery, the leaves of the control group are dried and yellow or die, and the cold damage symptoms of the arabidopsis plants of the treatment groups 1 and 2 are obviously relieved. As can be seen from table 4, the survival rates of the treatment groups 1 and 2 after 3 days of recovery were significantly higher than those of the control group, wherein the treatment group 1 had the best effect and the survival rate was 95.0%. The results show that the atropine aqueous solution with the concentration range of 10-30 nmol/strain can relieve the freezing damage stress of arabidopsis.

TABLE 3 treatment concentration of the alkaloid atropine

TABLE 4 Arabidopsis thaliana survival rate statistics table

Claims

1. The application of the alkaloid atropine in relieving the freeze injury stress of plant seedlings.

2. The use of claim 1, wherein said young plants comprise young plants of canola and arabidopsis.

3. Use according to claim 2, characterized in that rape seedlings are root-soaked with an aqueous solution of the alkaloid atropine in the range of 50-150nmol per plant.

4. The use as claimed in claim 2, wherein Arabidopsis seedlings are irrigated with 10-30 nmol/strain of an aqueous solution of the alkaloid atropine.