CN111514053A

CN111514053A - Composition with acne removing effect and preparation method and application thereof

Info

Publication number: CN111514053A
Application number: CN201911075511.9A
Authority: CN
Inventors: 张青; 李洪海; 向琴; 汤仲标; 周雪怡
Original assignee: Tianjin Niqu Biotechnology Co ltd; Guangzhou Niqu Cosmetics Co ltd
Current assignee: Tianjin Niqu Biotechnology Co ltd; Guangzhou Niqu Cosmetics Co ltd
Priority date: 2019-11-06
Filing date: 2019-11-06
Publication date: 2020-08-11
Anticipated expiration: 2039-11-06
Also published as: CN111514053B

Abstract

The invention belongs to the field of cosmetics, and relates to a composition with an acne removing effect, and a preparation method and application thereof. The composition comprises a lactobacillus fermentation product, a filtrate of a leuconostoc/radish root fermentation product, and a white willow bark extract. According to the composition, the lactobacillus fermentation product, the leuconostoc/radish root fermentation product filtrate, the saccharide isomerate and the white willow bark extract are combined to form the composition, and the components have synergistic effect, so that the proliferation of propionibacterium acnes is effectively competitively inhibited, the health of a microorganism group is balanced, the skin repair is promoted, the skin is tightened, acne marks are reduced, the skin quality is improved, and the regeneration of acnes is prevented. Meanwhile, the composition disclosed by the invention has the effects of enhancing the skin barrier, promoting the effective absorption of active skin care ingredients by the skin, further nourishing the skin, and keeping the skin moist, fine and smooth.

Description

Composition with acne removing effect and preparation method and application thereof

Technical Field

The invention belongs to the field of cosmetics, and relates to a composition with an acne removing effect, and a preparation method and application thereof.

Background

Acne (whelk) is a chronic inflammatory dermatosis of a pilosebaceous unit, and is shown by the combined investigation of the national dermatosis association and the national association of teenagers: the incidence of the acne in China is on the trend of increasing year by year, and more than 80% of people can encounter the acne in different degrees. Wherein the number of acne patients of middle school students is 2.94 hundred million, the number of acne patients of college students is 1.33 hundred million, the number of acne patients of office white collar of over 25 years old is more than 1.27 hundred million, and the total of three majority of people is more than 5.5 hundred million. Acne seriously affects the appearance and confidence of people and has negative influence on the work and life of people, so the acne removal is one of the research focuses of cosmetics.

The occurrence of whelk is closely related to factors such as excessive sebum secretion, blockage of pilosebaceous ducts, bacterial infection, inflammatory reaction and the like. A variety of microorganisms are present in hair follicles, and when Propionibacterium acnes proliferates in large quantities, the lipase produced by Propionibacterium acnes breaks down sebum to produce free fatty acids, while chemotactic inflammatory cells and mediators, inducing and exacerbating the inflammatory response. Therefore, propionibacterium acnes is considered as a main bacterium for producing acnes, and most of traditional acne-removing products achieve the aim of removing acnes by killing propionibacterium acnes.

However, researches show that the propionibacterium acnes also exists on healthy human skin, if the propionibacterium acnes is killed once, the ecological system of microorganisms on the skin epidermis is unbalanced, the problem of the pox is not solved fundamentally, and certain microorganisms can be resistant to products to cause more skin problems.

Disclosure of Invention

The present invention aims to provide a novel composition.

Another object of the present invention is to provide a composition having acne removing efficacy.

The invention also aims to provide a cosmetic with good acne removing effect.

The invention also aims to provide the acne-removing cosmetic which has the effects of improving the skin grease amount, being mild, having a good acne-removing effect, moisturizing and smoothing the skin.

In order to solve the above technical problems, in one aspect, the present invention provides a composition comprising a lactobacillus fermentation product, a leuconostoc/radish root fermentation product filtrate, and a white willow bark extract.

In some embodiments, the composition further comprises saccharide isomers.

In some embodiments, the composition comprises the following components in parts by weight: 30-50 parts of lactobacillus fermentation product, 10-30 parts of leuconostoc/radish root fermentation product filtrate, 10-30 parts of white willow bark extract and 5-20 parts of saccharide isomer.

In some embodiments, the parts by weight of the lactobacillus fermentation product may be 30 parts, 33 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 47 parts, and 50 parts.

In some embodiments, the weight fraction of the leuconostoc/radish root fermentation product filtrate may be 10%, 12%, 15%, 17%, 20%, 22%, 25%, 27%, 30%.

In some embodiments, the weight fraction of the white willow bark extract may be, for example, 10%, 12%, 15%, 17%, 20%, 22%, 25%, 27%, 30%.

In some embodiments, the weight fraction of saccharide isomers may be, for example, 5%, 8%, 10%, 12%, 15%, 18%, 20%.

In some embodiments, the composition comprises the following components in parts by weight: 35-47 parts of lactobacillus fermentation product, 12-27 parts of leuconostoc/radish root fermentation product filtrate, 12-25 parts of white willow bark extract and 8-18 parts of saccharide isomer.

In some embodiments, the following components are included in parts by weight: 38-45 parts of lactobacillus fermentation product, 15-25 parts of leuconostoc/radish root fermentation product filtrate, 15-22 parts of white willow bark extract and 10-15 parts of saccharide isomer. In another aspect, the present invention provides a method of preparing the composition, comprising the steps of: respectively weighing lactobacillus fermented product, filtrate of Leuconostoc/radix Raphani fermented product, white willow bark extract, and saccharide isomer, mixing, stirring at constant temperature, and filtering.

In some embodiments, the constant temperature is 25-40 ℃; for example, the temperature may be 25 ℃, 27 ℃, 30 ℃, 33 ℃, 35 ℃, 37 ℃, 40 ℃, preferably 35 ℃.

In some embodiments, the stirring speed is 300-500rpm, for example, 300rpm, 320rpm, 350rpm, 370rpm, 400rpm, 420rpm, 450rpm, 470rpm, 500rpm, preferably 400 rpm.

In some embodiments, the filtration pore size is 200-600 mesh; for example, the mesh size may be 200 mesh, 300 mesh, 400 mesh, 500 mesh, 600 mesh, preferably 500 mesh.

In another aspect, the present invention provides the use of said composition in cosmetics.

In the present invention, the cosmetic may be a cream, an emulsion, an essence, a lotion, a gel or a mask, without specific limitation.

In some embodiments, the cosmetic is a gel, lotion, or serum.

In some embodiments, the cosmetic is in an anti-acne product.

In another aspect, the present invention provides a cosmetic comprising the composition.

In some embodiments, the composition is present in the cosmetic product in an amount of 0.5 to 20% by weight.

In some embodiments, the composition is present in the cosmetic product in an amount of 5 to 15% by weight. In some embodiments, the composition is present in the cosmetic product in an amount of from 8 to 12% by weight.

In some embodiments, in a cosmetic, the cosmetic comprises the following components in weight percent: 0.5-20% of the composition; 0.1-8% of auxiliary phase; 76-94% of water phase. The weight percentage of the composition may be, for example, 0.5%, 1%, 2%, 5%, 8%, 10%, 12%, 15%, 17%, 20%, etc.

In some embodiments, the cosmetic comprises the following components in weight percent: 5-15% of the composition; 0.3-5% of auxiliary phase; 85-90% of water phase.

In some embodiments, the cosmetic comprises the following components in weight percent: 3-5% of lactobacillus fermentation product, 1-3% of filtrate of Leuconostoc/radish root fermentation product, 1-3% of white willow bark extract and 0.5-2% of saccharide isomer; 0.1-8% of auxiliary phase; 76-94% of water phase.

In some embodiments, the cosmetic comprises the following components in percentage by weight: 3-5% of lactobacillus fermentation product, 1-3% of filtrate of Leuconostoc/radish root fermentation product, 1-3% of white willow bark extract, 0.5-2% of saccharide isomer, 0.1-5% of panthenol, 0.05-2% of allantoin, 0.05-5% of sodium hyaluronate, 1-10% of butanediol, 0.1-0.5% of aminomethyl propanol and water to 100%.

In some embodiments, the cosmetic is a serum, and the aqueous phase comprises any one of glycerin, butylene glycol, propylene glycol, panthenol, allantoin, xanthan gum, sclerotium rolfsii, hydroxyethyl cellulose, carbomer 940, carbomer 941, acrylic/C10-30 alkanol acrylate crosspolymer, sodium hyaluronate, beta glucan, and water, or a combination of at least two thereof, preferably a combination of glycerin, panthenol, allantoin, xanthan gum, carbomer 941, sodium hyaluronate, butylene glycol, and water.

Preferably, the auxiliary phase comprises any one of methyl hydroxybenzoate, propyl hydroxybenzoate, p-hydroxyacetophenone, phenoxyethanol, aminomethyl propanol, triethanolamine, tromethamine, or a combination of at least two of them; preferably a combination of p-hydroxyacetophenone, aminomethylpropanol, phenoxyethanol.

Preferably, the composition is added in the serum in an amount of 3-15 wt%, for example, 3%, 5%, 7%, 10%, 13%, 15%.

In some embodiments, the cosmetic is a gel.

Preferably, the aqueous phase comprises any one of glycerol, butylene glycol, propylene glycol, panthenol, allantoin, carbomer 940, carbomer 30, carbomer 21, xanthan gum, sclerostin, hydroxyethyl cellulose, sodium hyaluronate, hydroxyethyl urea and water, or a combination of at least two, preferably a combination of glycerol, panthenol, allantoin, carbomer 30, sodium hyaluronate, sclerostin and water.

Preferably, the auxiliary phase comprises any one of methyl hydroxybenzoate, propyl hydroxybenzoate, p-hydroxyacetophenone, phenoxyethanol, aminomethyl propanol, triethanolamine, tromethamine and dipotassium glycyrrhizinate, or a combination of at least two of them, preferably a combination of p-hydroxyacetophenone, aminomethyl propanol, phenoxyethanol and dipotassium glycyrrhizinate.

Preferably, the composition is added in the jelly cream in an amount of 5-15 wt%, for example, 5%, 7%, 10%, 13%, 15%.

In some embodiments, the cosmetic further comprises an oil phase in an amount of 0-5% by weight.

In some embodiments, the cosmetic is an emulsion.

Preferably, the aqueous phase comprises any one or a combination of at least two of panthenol, allantoin, disodium EDTA, xanthan gum, sclerotium rolfsii, hydroxyethyl cellulose, sodium hyaluronate, betaine, carbomer 940, carbomer 941, acrylic/C10-30 alkanol acrylate crosspolymer, glycerol, propylene glycol, butylene glycol, pentylene glycol and water.

As a preferred embodiment, the aqueous phase is a combination of panthenol, allantoin, disodium EDTA, xanthan gum, sodium hyaluronate, acrylic/C10-30 alkanol acrylate crosspolymer, water, butylene glycol and pentylene glycol.

Preferably, the oil phase comprises any one of polydimethylsiloxane, hydrogenated polydecene, tocopheryl acetate, caprylic/capric triglyceride, dioctyl carbonate, cetearyl alcohol, glyceryl stearate, hydrogenated lecithin, polysorbate-20, PEG-20 methyl glucose sesquistearate, methylparaben, propylparaben, or a combination of at least two thereof, preferably polydimethylsiloxane, tocopheryl acetate, caprylic/capric triglyceride, cetearyl alcohol, glyceryl stearate, hydrogenated lecithin, polysorbate-20, propylparaben.

Preferably, the auxiliary phase comprises any one of sodium benzoate, phenoxyethanol, aminomethyl propanol, triethanolamine, tromethamine, or a combination of at least two thereof, preferably phenoxyethanol, aminomethyl propanol.

Preferably, the composition with acne removing efficacy is added in the emulsion in an amount of 5-15 wt%, for example, 5%, 7%, 10%, 13%, 15%.

In still another aspect, the present invention provides a method for preparing the above cosmetic, comprising the steps of:

(1) heating water, panthenol, allantoin, sodium hyaluronate, and butanediol to 50-90 deg.C, stirring, and homogenizing to completely dissolve;

(2) keeping the temperature for 10-15min, cooling to below 40 deg.C, adding aminomethyl propanol and the composition of any one of claims 1-4, stirring and homogenizing;

(5) discharging after the inspection is qualified.

Compared with the prior art, one embodiment of the invention has the beneficial effects that:

(1) according to the composition, the lactobacillus fermentation product, the leuconostoc/radish root fermentation product filtrate, the saccharide isomerate and the white willow bark extract are combined to form the composition, and the components have synergistic effect, so that the proliferation of propionibacterium acnes is effectively competitively inhibited, the health of a microorganism group is balanced, the skin repair is promoted, the skin is tightened, acne marks are reduced, the skin quality is improved, and the regeneration of acnes is prevented.

(2) Meanwhile, the composition provided by the invention enhances the skin barrier, promotes effective absorption of active skin care ingredients by the skin, further nourishes the skin, and keeps the skin moist, fine and smooth.

Drawings

FIG. 1 is a graph showing the change of the amount of skin oil with time using the essences of example 5 and comparative example 17; example 5 on the left; comparative example 17 is on the right.

Detailed Description

The technical solutions of the present invention are further illustrated by the following specific examples, which do not represent limitations to the scope of the present invention. Insubstantial modifications and adaptations of the present invention by others of the concepts fall within the scope of the invention.

Lactobacillus fermentation products were purchased from CLR, Germany

The S.streptococci/radix Raphani fermentation product filtrate was purchased from Ti-Ti science Co., Ltd

White willow bark extract purchased from orange twigs technologies ltd

Saccharide isomerate was purchased from Dismann Limited, Netherlands

Example 1 formula of composition for acne treatment efficacy and preparation method thereof

The composition with the acne removing effect comprises the following components in percentage by mass:

45% of lactobacillus fermentation product, 25% of a filtrate of a kefir/radish root fermentation product, 15% of white willow bark extract and 15% of saccharide isomer.

The preparation method comprises the following steps:

weighing lactobacillus fermentation product, Leuconostoc/radix Raphani fermentation product filtrate, white willow bark extract, and saccharide isomer according to the above proportion, controlling temperature at 35 deg.C, controlling stirring speed at 300rpm, stirring well, and filtering with 400-mesh micropore.

Example 2 formulation of the composition and method of preparation

40% of lactobacillus fermentation product, 25% of a filtrate of a kefir/radish root fermentation product, 15% of white willow bark extract and 20% of saccharide isomer.

The preparation method comprises the following steps:

weighing lactobacillus fermentation product, Leuconostoc/radix Raphani fermentation product filtrate, white willow bark extract, and saccharide isomer according to the above proportion, controlling temperature at 35 deg.C, controlling stirring speed at 400rpm, stirring well, and filtering with 400-mesh micropore.

Example 3 formulation of the composition and method of preparation

50% of lactobacillus fermentation product, 20% of filtrate of staphylococcus aureus/radish root fermentation product, 20% of white willow bark extract and 10% of saccharide isomer.

The preparation method comprises the following steps:

weighing lactobacillus fermentation product, Leuconostoc/radix Raphani fermentation product filtrate, white willow bark extract, and saccharide isomer according to the above proportion, controlling temperature at 40 deg.C, controlling stirring speed at 400rpm, stirring well, and filtering with 500-mesh micropore.

Comparative example 1

The same conditions as in example 1 were used except that the fermentation product of lactobacillus was 25%, the filtrate of fermentation product of leuconostoc/radish root was 35%, the extract of white willow bark was 7%, and the saccharide isomer was 33% as compared with example 1.

Comparative example 2

The same conditions as in example 1 were used except that the fermentation product of lactobacillus was 60%, the filtrate of fermentation product of leuconostoc/radish root was 8%, the extract of white willow bark was 5%, and the saccharide isomer was 27% as compared with example 1.

Comparative example 3

The conditions were the same as in example 1 except that only the lactic acid bacteria fermentation product was added, as compared with example 1.

Comparative example 4

The conditions were the same as in example 1 except that only the filtrate of the fermentation product of Leuconostoc/radish root was added, as compared with example 1.

Comparative example 5

The procedure of example 1 was followed except that only the white willow bark extract was added, as compared with example 1.

Comparative example 6

The conditions were the same as in example 1 except that only the saccharide isomorphs were added as compared with example 1.

Comparative example 7

The procedure of example 1 was repeated except that no lactobacillus fermentation product was added, and 50% of a filtrate of a fermentation product of leuconostoc/radish root, 30% of an extract of white willow bark, and 20% of a saccharide isomer were added, as compared with example 1.

Comparative example 8

The procedure of example 1 was followed except that the filtrate of the fermentation product of Leuconostoc/radish root was not added, and the fermentation product of Lactobacillus was added in an amount of 50%, the extract of white willow bark was added in an amount of 30%, and the saccharide isomer was added in an amount of 20%.

Comparative example 9

The procedure of example 1 was repeated except that the white willow bark extract was not added, and 50% lactobacillus fermented product, 30% leuconostoc/radish root fermented product filtrate, and 20% saccharide isomer were added, as compared with example 1.

Comparative example 10

The conditions were the same as in example 1 except that no sugar isomer was added, and that 40% of lactobacillus fermented product, 30% of staphylococcus aureus/radish root fermented product filtrate, and 30% of white willow bark extract were added, as compared with example 1.

Comparative example 11

Compared with example 1, the conditions were the same as example 1 except that the lactic acid bacteria fermented extract was used instead of the lactic acid bacteria fermented product in example 1.

Comparative example 12

The same conditions as in example 1 were used except that the Bacillus/glutamic acid fermentation product filtrate was used instead of the Leuconostoc/radish root fermentation product filtrate in example 1, as compared with example 1.

Comparative example 13

The same conditions as in example 1 were used, except that the white willow bark extract in example 1 was replaced with a white willow flower extract, as compared with example 1.

Comparative example 14

Compared to example 1, the conditions were the same as in example 1 except that trehalose was used instead of the saccharide isomer in example 1.

Test example 1 competitive inhibition test for Propionibacterium acnes

In vitro testing:

examples 1-3 and comparative examples 1-14 were applied to skin microorganisms cultured in vitro to verify their competitive inhibitory effect against acnes.

The test method is as follows:

(1) respectively inoculating Staphylococcus epidermidis to nutrient broth culture medium (pharmacopeia), and inoculating Propionibacterium acnes to GAM culture solution.

(2) 10ml of each culture broth, 9 portions each, were added with 0.1ml of the compositions of examples 1-3 and comparative examples 1-6, respectively, and 0.1ml of deionized water (blank).

(3) Then, the staphylococcus epidermidis is aerobically cultured at 37 ℃ for 24h, and the propionibacterium acnes is anaerobically cultured at 37 ℃ for 48 h.

(4) The number of each strain in the culture solution before and after the culture was determined by the dilution plate method. The measurement results are shown in table 1.

TABLE 1 comparison of skin microbial counts before and after incubation (cfu/ml)

As can be seen from table 1, the lactobacillus fermentation product, the leuconostoc/radish root fermentation product filtrate, the white willow bark extract and the saccharide isomer in the mixture ratio range of the invention can effectively and competitively inhibit the proliferation of acne bacillus and promote the proliferation of beneficial bacteria (represented by staphylococcus epidermidis). Comparing example 1 with comparative examples 1-2, it can be seen that the addition of too much or too little lactobacillus fermentation product or leuconostoc/radish root fermentation product filtrate can affect the competitive inhibition of the proliferation of acnes; comparing example 1 with comparative examples 3-6, it can be seen that the competitive inhibition of the proliferation of acne bacillus by a single component is not obvious, and the competitive inhibition of the proliferation of acne bacillus can be effectively achieved only by the synergistic effect under the condition of the ratio of the components. Comparing example 1 with comparative examples 7-10, it can be seen that the absence of any component in the present invention affects the proliferation of beneficial bacteria (represented by staphylococcus epidermidis), and has no obvious effect on the competitive inhibition of the proliferation of acne bacillus, and each component supplements each other and cannot be absent. Comparing example 1 with comparative examples 11 to 14, it can be seen that any of the components of the present invention was replaced with a similar substance, which resulted far less than example 1, and that any of the components of the present invention was not replaceable. Comprehensively, the lactobacillus fermentation product, the leuconostoc/radish root fermentation product filtrate, the white willow bark extract and the saccharide isomer supplement each other, are all indispensible and irreplaceable, can effectively competitively inhibit the proliferation of acne bacillus within a mixture ratio range, and promote the proliferation of beneficial bacteria (represented by staphylococcus epidermidis).

Example 4 emulsion containing a composition having anti-acne efficacy

The composition with the acne removing effect of the optimal example 2 is used for preparing the emulsion, and the specific formula is shown in the following table 2:

table 2 formula table of emulsion containing a composition having acne removing effect

The preparation method of the emulsion comprises the following steps:

(1) putting phase A into an emulsifying pot, heating to 80-85 deg.C, stirring, and homogenizing to completely dissolve;

(2) putting the phase B into an oil phase pot, heating to 80-85 ℃, and stirring to dissolve the phase B uniformly;

(3) pumping the oil phase pot material into an emulsifying pot, stirring, homogenizing and emulsifying for 10-20min to completely emulsify;

(4) keeping the temperature for 10-15min, cooling to about 40 deg.C, adding C phase material, stirring and homogenizing for 5-10 min;

(5) discharging after the inspection is qualified.

Comparative example 15

Compared to example 4, the conditions were the same as example 4 except that a composition having acne removing efficacy of comparative example 2 was used instead of a composition having acne removing efficacy of example 4.

Comparative example 16

Compared to example 4, the conditions were the same as example 4 except that a composition having anti-acne efficacy of comparative example 5 was used instead of a composition having anti-acne efficacy of example 4.

Test example 2 comparative test for the number of colonies on human skin

Human body trial test:

1. the test population:

60 patients with facial acne are selected, and the patients are required to be free of external application medicines within 1 month, are free of other facial diseases, are 18-30 years old, half of men and half of women, are randomized, and are divided into 3 groups, wherein each group comprises 10 men and 10 women, and is recorded as A, B, C groups. The face health adult 20, 10 men and 10 women, aged 18-30 years, were selected and recorded as group D.

2. The experimental steps are as follows:

2.1 Experimental methods

The following experiment was carried out using example 4, comparative example 15 and comparative example 16. The subjects in the A group and the D group are cleaned by water and then are smeared with the emulsion of the example 4; cleaning the face of the test subjects in the group B with clean water, and then applying the emulsion of the comparative example 15; the subjects in group C were cleaned with clean water and then applied with the emulsion of comparative example 16. Each group of subjects used the face-cleaning liquid once a day in the morning and evening, the test period was 30 days, and during the test period, the subjects stopped using other drugs and cosmetics, and kept the diet light and regular work and rest. The facial skin condition of the subjects before and after the test was recorded.

2.2 sample Collection

Before the test, after the face of the patient is washed by normal saline for 5 minutes, a PBS buffer solution is used for soaking a sterile cotton swab, the sterile cotton swab is slightly applied from inside to outside within the range of 4cm multiplied by 4cm of an acne skin lesion area, and the sterile cotton swab is immediately cut off by a sterile scissors after collection and placed in a centrifugal tube of 1mL sterile PBS. After the experiment, the skin lesion improvement area is sampled by the same method. Group D samples on the forehead of the subject.

2.3 inoculation culture

Diluting with PBS solution to 10 times by dilution method^-1～10^-66 dilutions, taking 0.01mL of each diluted solution, inoculating to a propionibacterium acnes and staphylococcus epidermidis selection culture medium, placing staphylococcus epidermidis at 37 ℃ for 24h aerobic culture, and placing propionibacterium acnes at 37 ℃ for 48h anaerobic culture.

The control subjects tried the average number of viable bacteria of Propionibacterium acnes and Staphylococcus epidermidis on the front and back faces, and the results are shown in Table 3.

TABLE 3 average number of viable Propionibacterium acnes and Staphylococcus epidermidis on the face of the subjects (cfu/cm)²)

As can be seen from the data in Table 3, the viable count of Propionibacterium acnes and Staphylococcus epidermidis before and after the group A test is close to that of healthy people, and the viable count of Propionibacterium acnes in the group B and the group C is greater than that of Staphylococcus epidermidis and is greatly different from that of healthy people. The above results show that the components of the composition with the acne removing effect have synergistic effect, and the lack of any component can obviously reduce the performance of the lotion.

Example 5 essence containing a composition having acne removing effect

The essence is prepared from the composition with the acne removing effect in the best example 2, and the specific formula is shown in the following table 4:

table 4 formula of essence containing a composition having acne removing effect

The preparation method of the essence comprises the following steps:

(1) putting the phase A into an emulsifying pot, heating to 80-85 ℃, and stirring and homogenizing until the phase A is uniformly dissolved;

(2) heating phase B to 60-70 deg.C, stirring to dissolve to transparent, slowly adding into emulsifying pot, and stirring;

(3) keeping the temperature for 10-15min, cooling to 40-45 deg.C, adding C phase material, stirring and homogenizing to obtain paste;

(4) discharging after the detection is qualified.

Comparative example 17

Compared with example 5, except that the composition with acne removing efficacy of example 2 is not added, the conditions are the same as

Example 5.

Test example 3 skin oil content test

Propionibacterium acnes is an anaerobic bacterium, and excessive oil secretion can cause the blockage of sebum hair follicles, so that a superior growth environment is provided for the Propionibacterium acnes, and the Propionibacterium acnes can be massively propagated to cause the formation of acne.

Using example 5 and comparative example 17, the following experiment was conducted, and 30 patients with acne on their faces were selected, and the age was 18 to 30 years, half of the age of male and female, and the patients were randomly divided into 2 groups, which were designated as group I and group II. Each group had 15 males and 15 females. The essence of example 5 was used in the test subjects of group I, the essence of comparative example 17 was used in the test subjects of group II, each test subject was used after cleansing the face in the morning and evening every day, the test period was 30 days, and during the test period, the test subjects stopped using other drugs and cosmetics, and kept the diet light and regular work and rest. The test area is measured with the instrument before the sample is tried and after 1 week, 2 weeks, 3 weeks, 4 weeks of trial. On the day of testing, the subject used no sample and needed to sit at constant temperature (22 + -1) ° c, (55 + -5)% constant humidity room for 30min and remain in a relaxed state, and the test results are shown in fig. 1.

As can be seen from figure 1, the composition with the acne removing effect can effectively balance the oil secretion of the skin, so that the mass propagation of propionibacterium acnes can be inhibited, and the production of acnes can be inhibited.

Example 6 gel containing a composition having anti-acne effect

The composition with the acne removing effect of the optimal example 2 is adopted to prepare the gel cream, and the specific formula is shown in the table 5:

table 5 gel formula containing composition with acne removing effect

The preparation method of the jelly cream comprises the following steps:

(4) discharging after the detection is qualified.

Comparative example 18

The conditions were the same as in example 6 except that a composition having anti-acne efficacy of example 2 was not added.

Comparative example 19

Any one of commercially available anti-acne gels is suitable for being applied to the whole face, contains effective substances such as sodium hyaluronate, panthenol, allantoin, dipotassium glycyrrhizinate and the like, and is declared to have a better anti-acne effect.

Test example 4 evaluation test on human body trial

1. Test population

60 people with acne on the face are selected without other facial diseases. Age 18-30 years, half of both men and women, were randomized into 3 groups of 10 men and 10 women. It is required to use cosmetics according to the standard, to be sensitive to the cosmetics and to make an accurate judgment after use.

2. Experimental methods

Using example 6 and comparative examples 18-19, each group of subjects was dosed daily with a face wash in the morning and evening for a period of 30 days during which subjects discontinued other medications and cosmetics and maintained a light diet and regular work and rest. And (3) evaluating the acne removal, oil control degree, humidity preservation, temperature and fineness of the product in the trial process, wherein the highest score is 10, and the lowest score is 1.

3. Test results

And tracking and recording the actual using effect of the sample, collecting feedback opinions of the testers, sorting and analyzing, and obtaining statistical results shown in table 6.

TABLE 6 average values of human trial results

	Acne removing effect	Oil control degree	Moisture retention	Degree of mildness	Fineness of fineness
						Example 6	9.03	8.62	8.44	9.06	8.24
Comparative example 18	3.78	2.17	6.43	9.02	2.35
						Comparative example 19	6.22	5.62	7.68	7.33	6.24

As can be seen from the results of table 6, comparing example 6 with comparative examples 18 to 19, the composition having the acne removing effect of the present invention can gently and effectively remove acne while improving the amount of oil and fat, moisture and smoothness of the skin.

In conclusion, the composition with the acne removing effect can effectively and competitively inhibit the proliferation of the acne bacillus only by the synergistic effect under the condition of the proportion of the components. The active bacteria number of the propionibacterium acnes and the staphylococcus epidermidis is close to that of healthy people, the skin oil quantity is improved, the acne is removed mildly and effectively, and the effects of moisturizing and refining the skin are achieved.

The present invention has been described in further detail with reference to specific embodiments, but the present invention is not limited to the above detailed description. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.

Claims

1. A composition comprises a Lactobacillus fermentation product, a Leuconostoc/Raphanus sativus root fermentation product filtrate, and an extract of white willow bark.

2. The composition of claim 1, wherein the composition further comprises saccharide isomers.

3. The composition of claim 2, comprising the following components in parts by weight: 30-50 parts of lactobacillus fermentation product, 10-30 parts of leuconostoc/radish root fermentation product filtrate, 10-30 parts of white willow bark extract and 5-20 parts of saccharide isomer.

4. The composition of claim 2, comprising the following components in parts by weight: 35-47 parts of lactobacillus fermentation product, 12-27 parts of leuconostoc/radish root fermentation product filtrate, 12-25 parts of white willow bark extract and 8-18 parts of saccharide isomer.

5. The composition of claim 2, comprising the following components in parts by weight: 38-45 parts of lactobacillus fermentation product, 15-25 parts of leuconostoc/radish root fermentation product filtrate, 15-22 parts of white willow bark extract and 10-15 parts of saccharide isomer.

6. A process for preparing a composition according to any one of claims 2 to 5, comprising the steps of:

respectively weighing lactobacillus fermentation product, filtrate of Leuconostoc/radix Raphani fermentation product, white willow bark extract, and saccharide isomerous body, mixing, stirring at constant temperature, and filtering;

preferably, the constant temperature is 25-40 ℃;

or preferably, the stirring speed is 300-;

or preferably, the filtering pore size is 200-600 meshes;

more preferably, the filter pore size is 500 mesh.

7. Use of a composition according to any one of claims 1 to 5 in cosmetics;

preferably, the cosmetic is cream, lotion, essence, aqua, gel or mask;

more preferably, the cosmetic is a gel, a lotion or a serum;

preferably, the cosmetic is an acne-removing product.

8. A cosmetic comprising the composition of any one of claims 1 to 5;

preferably, the weight percentage of the composition is 0.5-20%;

more preferably, the weight percentage of the composition is 5-15%;

even more preferably, the weight percentage of the composition is 8-12%.

9. The cosmetic of claim 8, further comprising panthenol, allantoin, sodium hyaluronate, butylene glycol, aminomethyl propanol, and water.

10. A method of preparing the cosmetic of claim 9, comprising the steps of:

(2) keeping the temperature for 10-15min, cooling to below 40 deg.C, adding aminomethyl propanol and the composition of any one of claims 1-4, stirring, and homogenizing.