CN111466395B

CN111466395B - Natural environment-friendly non-heading Chinese cabbage seed coating agent and preparation method thereof

Info

Publication number: CN111466395B
Application number: CN202010364546.0A
Authority: CN
Inventors: 侯喜林; 徐鸿飞; 王羚核; 李英; 周鸿章
Original assignee: Nanjing Ideal Agricultural Science And Technology Co ltd; Nanjing Agricultural University
Current assignee: Nanjing Ideal Agricultural Science And Technology Co ltd; Nanjing Agricultural University
Priority date: 2020-04-30
Filing date: 2020-04-30
Publication date: 2021-07-27
Anticipated expiration: 2040-04-30
Also published as: CN111466395A

Abstract

The invention discloses a natural environment-friendly coating agent for non-heading Chinese cabbage seeds and a preparation method thereof. The invention provides a natural environment-friendly coating agent for non-heading Chinese cabbage seeds, which comprises a film-forming agent, an initiator, an insecticidal bactericide, a warning color and water; the film forming agent is 2-10% of waxy corn starch, the initiator is 50mg/L or 80mg/L of ascorbic acid, the insecticidal bactericide is 20-300 mg/L of matrine, the warning color is 1-25% of iron oxide yellow, and the waxy corn starch is used as the film forming agent. The insecticidal bactericide is matrine, the material is natural and environment-friendly, the screened concentration and ascorbic acid interact, the activity of the non-heading Chinese cabbage seeds can be effectively improved, and the insecticidal bactericide has insecticidal and bactericidal effects and is more beneficial to the growth of seedlings. The warning color is iron oxide yellow, belongs to mineral pigment, is safe and environment-friendly, and is not easy to fade. The invention is environment-friendly and pollution-free, and can effectively improve the germination capacity of the non-heading Chinese cabbage seeds.

Description

Natural environment-friendly non-heading Chinese cabbage seed coating agent and preparation method thereof

Technical Field

The invention relates to the technical field of agriculture, in particular to a natural and environment-friendly coating agent for non-heading Chinese cabbage seeds and a preparation method thereof.

Background

The seed coating is a seed treatment technology which utilizes an adhesive or a film-forming agent to coat non-seed materials such as a bactericide, an insecticide, a micro-fertilizer, a plant growth regulator, a filling agent, a warning color and the like on the surface of seeds so as to enable the seeds to form spherical shapes or basically keep the original shapes, improve the stress resistance and disease resistance of the seeds, accelerate the germination speed of the seeds, promote the seedling formation, increase the yield and improve the product quality. Generally divided into seed coating and seed pelleting. The seed coating technology is that the film forming agent or adhesive is used to coat pesticide, micro fertilizer, plant hormone and other matter onto the seed surface to form one homogeneous film. The weight of the coated seeds is slightly increased, and the volume and the shape of the coated seeds are not obviously changed. Seed coating techniques are generally applicable to large and medium grain vegetable crop seeds such as soybean, corn, cucumber, and the like. The seed pelleting technology is characterized in that a film forming agent or an adhesive is utilized to attach substances such as insecticide, trace fertilizer, phytohormone and the like to the surface of seeds through mechanical treatment, so that pelleted seeds with smooth appearance, increased particles and obvious changes in volume and weight are formed. Generally applicable to small, light and irregularly shaped vegetable crop seeds, such as: carrot, tomato, radish, pepper, cabbage, etc.

Non-heading Chinese cabbage (Brassica rapa ssp. chinensis) belongs to Brassicaceae Brassica subspecies, commonly called green vegetables, pakchoi, rape and the like, and is an important leaf vegetable crop native to China. The non-heading Chinese cabbage is fresh and tender, rich in nutrition, deeply popular with people and plays an important role in planting and consuming vegetables in China. The non-heading Chinese cabbage is mostly cultivated in a direct seeding mode, the seed particles are small, the practical production problems of large seeding quantity, low emergence rate, poor seedling quality and the like exist, and therefore, the improvement of the seed activity and the seedling quality have direct influence on the yield and the quality of the non-heading Chinese cabbage.

There have been many domestic patent applications relating to vegetable seed coating technology, and patent No. CN108850025A discloses a vegetable seed coating agent and its preparation method, and patent No. CN104478584A discloses a cruciferous vegetable seed coating agent and its preparation method; patent No. CN101238767 discloses a method for pelleting and coating cruciferous vegetable seed plant source pesticides; patent No. CN102584482A discloses a pollution-free Chinese cabbage seed coating agent; the patent No. CN102084741A discloses a method for processing green cabbage seeds, however, the above-mentioned seed coating method is not well adapted to the coating of non-heading cabbage seeds, and currently, there are few researches on the coating of non-heading cabbage seeds, so there is a need to provide a natural and environment-friendly coating agent for non-heading cabbage seeds and a preparation method thereof.

Disclosure of Invention

The invention provides a natural and environment-friendly coating agent for non-heading Chinese cabbage seeds and a preparation method thereof, which can improve the vitality and the commodity value of the non-heading Chinese cabbage seeds.

In a first aspect, the invention provides a natural and environment-friendly coating agent for non-heading Chinese cabbage seeds, which is characterized by comprising a film-forming agent, an initiator, an insecticide and bactericide, a warning color and water;

the film forming agent is 2-10% of waxy corn starch, the initiator is 50mg/L or 80mg/L of ascorbic acid, the insecticidal bactericide is 20-300 mg/L of matrine, and the warning color is 1-25% of iron oxide yellow.

Optionally, the film forming agent is waxy corn starch 3.3%, the initiator is ascorbic acid 80mg/L, the insecticidal bactericide is matrine 120mg/L, and the warning color is iron oxide yellow 20%.

In a second aspect, the invention provides a preparation method of a natural and environment-friendly coating agent for non-heading Chinese cabbage seeds, which comprises the following steps:

weighing a certain mass of matrine and ascorbic acid to prepare a solution of matrine with the concentration of 20 mg/L-300 mg/L and 50mg/L or 80mg/L ascorbic acid;

step two, dissolving 2-10% of waxy corn starch in the solution obtained in the step one, and fully stirring to fully dissolve the waxy corn starch;

and step three, dissolving 1-25% of iron oxide yellow in the solution obtained in the step two, and fully stirring to ensure that the iron oxide yellow is uniformly distributed in the starch solution.

And step four, coating the non-heading Chinese cabbage seeds by using a seed coating machine.

Optionally, in the step one, a certain amount of matrine and ascorbic acid are weighed to prepare a matrine solution with a concentration of 120mg/L and an ascorbic acid solution with a concentration of 80 mg/L.

Optionally, in the second step, 3.3% waxy corn starch is dissolved in the solution obtained in the first step, and the solution is fully stirred to fully dissolve the waxy corn starch.

Optionally, in the third step, 20% of iron oxide yellow is dissolved in the solution obtained in the second step, and the solution is fully stirred, so that the iron oxide yellow is uniformly distributed in the starch solution.

Optionally, in the fourth step, the non-heading Chinese cabbage seeds are coated by using a seed coating machine according to the seed ratio of 1: 10.

The invention has the following beneficial effects: according to the natural environment-friendly coating agent for the non-heading Chinese cabbage seeds and the preparation method thereof, provided by the invention, waxy corn starch is used as a film forming agent, and the material is safe, environment-friendly, edible and low in cost. The insecticidal bactericide is matrine, the material is natural and environment-friendly, the screened concentration and ascorbic acid interact, the activity of the non-heading Chinese cabbage seeds can be effectively improved, and the insecticidal bactericide has insecticidal and bactericidal effects and is more beneficial to the growth of seedlings. The warning color is iron oxide yellow, belongs to mineral pigment, is safe and environment-friendly, and is not easy to fade. The natural environment-friendly coating agent for the seeds of the non-heading Chinese cabbages and the preparation method thereof are environment-friendly and pollution-free, and can effectively improve the germination capacity of the seeds of the non-heading Chinese cabbages.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a flow chart of a method for preparing a natural and environment-friendly coating agent for non-heading Chinese cabbage seeds according to the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.

The invention provides a natural environment-friendly coating agent for non-heading Chinese cabbage seeds, which comprises a film-forming agent, an initiator, an insecticidal bactericide, a warning color and water. The film forming agent is 2-10% of waxy corn starch, the initiator is 50mg/L or 80mg/L of ascorbic acid, the insecticidal bactericide is 20-300 mg/L of matrine, and the warning color is 1-25% of iron oxide yellow.

Preferably, the film forming agent is 3.3% of waxy corn starch, the initiator is 80mg/L of ascorbic acid, the insecticidal bactericide is 120mg/L of matrine, and the warning color is 20% of iron oxide yellow. The waxy corn starch is used as the film forming agent, and the material is safe, environment-friendly, edible and low in cost. The insecticidal bactericide is matrine, the material is natural and environment-friendly, the screened concentration and ascorbic acid interact, the activity of the non-heading Chinese cabbage seeds can be effectively improved, and the insecticidal bactericide has insecticidal and bactericidal effects and is more beneficial to the growth of seedlings. The warning color is iron oxide yellow, belongs to mineral pigment, is safe and environment-friendly, and is not easy to fade.

As shown in figure 1, the invention provides a preparation method of a natural and environment-friendly coating agent for non-heading Chinese cabbage seeds, which comprises the following steps:

step one, weighing a certain mass of matrine and ascorbic acid to prepare a solution of matrine with the concentration of 20 mg/L-300 mg/L and 50mg/L or 80mg/L ascorbic acid.

And step two, dissolving 2-10% of waxy corn starch in the solution obtained in the step one, and fully stirring to fully dissolve the waxy corn starch.

Specifically, in the first step, a certain amount of matrine and ascorbic acid are weighed to prepare a matrine solution with a concentration of 120mg/L and an ascorbic acid solution with a concentration of 80 mg/L. In the second step, 3.3% waxy corn starch is dissolved in the solution obtained in the first step, and the solution is fully stirred to fully dissolve the waxy corn starch. And in the third step, dissolving 20% of iron oxide yellow in the solution obtained in the second step, and fully stirring to ensure that the iron oxide yellow is uniformly distributed in the starch solution. In the fourth step, the non-heading Chinese cabbage seeds are coated by using a seed coating machine according to the medicine seed ratio of 1: 10.

The method for preparing the natural and environment-friendly coating agent for the seeds of the non-heading Chinese cabbage according to the present invention will be described in detail with reference to several specific examples.

Example 1

120mg of matrine, 80mg of ascorbic acid, 66.6g of waxy corn starch (starch: water: 1:15) and 200g of iron oxide yellow are weighed and dissolved in 1L of distilled water, and the mixture is fully stirred to be completely dissolved. 100g of seeds were coated with 10ml of the prepared coating agent. After natural air drying, a layer of yellow bright film is arranged outside the seeds, but the film surface is rough, and the seeds are bonded with each other frequently.

Example 2

Dissolving 120mg of matrine, 80mg of ascorbic acid, 50g of waxy corn starch (starch: water: 1:20) and 100g of iron oxide yellow in 1L of distilled water, and fully stirring to completely dissolve the materials. 100g of seeds were coated with 10ml of the prepared coating agent. After natural air drying, the seeds are coated with a layer of yellow bright film, but the surfaces are rough, and the seeds are mutually bonded.

Example 3

Dissolving 120mg of matrine, 80mg of ascorbic acid, 40g of waxy corn starch (starch: water: 1:25) and 200g of iron oxide yellow in 1L of distilled water, and fully stirring to completely dissolve the materials. 100g of seeds were coated with 10ml of the prepared coating agent. After natural air drying, the seeds are coated with a layer of yellow and bright film, the surface is smooth, and the seeds are less bonded with each other.

Example 4

Dissolving 120mg of matrine, 80mg of ascorbic acid, 33.3g of waxy corn starch (starch: water: 1:30) and 200g of iron oxide yellow in 1L of distilled water, and stirring to dissolve completely. 100g of seeds were coated with 10ml of the prepared coating agent. After natural air drying, the seeds are coated with a layer of yellow and bright film with smooth surface, and the seeds are basically not bonded with each other.

Example 5

120mg of matrine, 80mg of ascorbic acid, 28.6g of waxy corn starch (starch: water: 1:35) and 200g of iron oxide yellow are weighed and dissolved in 1L of distilled water, and the mixture is fully stirred to be completely dissolved. 100g of seeds were coated with 10ml of the prepared coating agent. After natural air drying, the seeds are coated with a layer of yellow and bright film, the surface is smooth, and the seeds are less mutually bonded.

Example 6

120mg of matrine, 80mg of ascorbic acid, 33.3g of waxy corn starch (starch: water: 1:30) and 100g of iron oxide yellow are weighed and dissolved in 1L of distilled water, and the mixture is fully stirred to be completely dissolved. 100g of seeds were coated with 10ml of the prepared coating agent. After natural air drying, the seeds are coated with a layer of dark yellow film with smooth surface and basically no bonding among the seeds.

The germination test is carried out on the non-heading Chinese cabbage seeds prepared by the preparation method of the natural environment-friendly non-heading Chinese cabbage seed coating agent.

1. Seed germination test:

the seeds were transferred to corresponding 90mm petri dishes, 50 particles were placed in each treatment, 10 parallel experiments were performed in each treatment, and the cultures were incubated at room temperature. Water was poured every 12 hours, keeping the filter paper moist.

2. Determination of germination index

And 3 germination indexes of the seeds, namely germination vigor, germination rate and germination index, are measured. The calculation method of each index is as follows:

seed germination (%) — number of germinated seeds at initial stage (day 3)/number of test seeds × 100%.

The germination rate (%) of the seeds was 100% of the number of germinated seeds/test seeds at the final stage (day 7).

Germination Index (GI) ═ Σ Gt/Dt.

Wherein Gt is the number of germinated seeds on different germination days (count of 7d), and Dt is the corresponding germination days.

3. Results and analysis

The treatment method of example 4 with the best appearance effect is selected in consideration of commodity, germination tests are carried out according to the method mentioned in 1 on 6 samples of non-heading Chinese cabbage 'yellow rose' seeds treated according to example 4, and the test results are recorded as shown in tables 1, 2, 3, 4, 5 and 6.

Control group: germination tests were carried out on 6 samples of non-heading Chinese cabbage 'yellow rose' seeds from different origins according to the method mentioned in 1, and the test results were recorded as shown in tables 1, 2, 3, 4, 5, and 6.

Table 1 effect of example 4 method treatment on seed germination of sample "one

Treatment of	Germination vigor (%)	Percentage of germination (%)	Index of germination
				Huang Meigui	100.00％a A	100.00％a A	129.44a A
Control group	92.40％b B	99.00％a A	99.66b B

Note: small letters in the data in the same column indicate that there was a significant difference at the 0.05 level between treatments; capital letters indicate significant differences between treatments at the 0.01 level. The same applies below.

Table 2 example 4 effect of method treatment on seed germination of sample "No. two

Treatment of	Germination vigor (%)	Percentage of germination (%)	Index of germination
				Huang Meigui	99.80％a A	99.80％a A	128.63a A
Control group	90.40％c B	98.40％a A	94.98b B

Table 3 effect of example 4 method treatment on seed germination of sample "No. three

Treatment of	Germination vigor (%)	Percentage of germination (%)	Index of germination
				Huang Meigui	100.00％a A	100.00％a A	128.29a A
Control group	93.80％b B	99.80％a A	97.94b B

Table 4 effect of example 4 method treatment on seed germination for sample "No. four

Treatment of	Germination vigor (%)	Percentage of germination (%)	Index of germination
				Huang Meigui	99.60％a A	100.00％a A	114.58b B
Control group	98.40％a A	99.60％a A	86.37b B

Table 5 effect of example 4 method treatment on seed germination for sample "five

Treatment of	Germination vigor (%)	Percentage of germination (%)	Index of germination
				Huang Meigui	100.00％a A	100.00％a A	128.89a A
Control group	99.40％a A	100.00％a A	109.62b B

Table 6 effect of example 4 method treatment on seed germination for sample "number six

As can be seen from the table 6, the germination vigor, the germination rate and the germination index of the 6 yellow rose non-heading Chinese cabbage seeds with different production areas are improved to a certain extent after the seeds are treated by the method 4. After coating treatment, the germination potential of 6 samples reaches 99.60 percent at the lowest, and the germination potential of the rest samples reaches 100 percent except the germination rate of No. 2 seeds of 99.80 percent. The germination vigor of 6 samples is increased by 7.60%, 9.40%, 6.20%, 1.20%, 0.60% and 1.00% in sequence compared with the control, the germination rate is increased by 1.00%, 1.40%, 0.20%, 0.40%, 0% and 0.20% in sequence, and the germination indexes are increased by 29.78, 33.65, 30.35, 28.21, 19.27 and 14.29 in sequence.

In conclusion, the natural and environment-friendly coating agent for the non-heading Chinese cabbage seeds and the preparation method thereof provided by the invention use waxy corn starch as a film forming agent, and the material is safe, environment-friendly, edible and low in cost. The insecticidal bactericide is matrine, the material is natural and environment-friendly, the screened concentration and ascorbic acid interact, the activity of the non-heading Chinese cabbage seeds can be effectively improved, and the insecticidal bactericide has insecticidal and bactericidal effects and is more beneficial to the growth of seedlings. The warning color is iron oxide yellow, belongs to mineral pigment, is safe and environment-friendly, and is not easy to fade. The natural environment-friendly coating agent for the seeds of the non-heading Chinese cabbages and the preparation method thereof are environment-friendly and pollution-free, and can effectively improve the germination capacity of the seeds of the non-heading Chinese cabbages.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A natural environment-friendly coating agent for non-heading Chinese cabbage seeds is characterized by comprising a film-forming agent, an initiator, an insecticidal bactericide, a warning color and water;

the film forming agent is waxy corn starch with the concentration of 3.3%, the waxy corn starch is 33.3g, and the mass ratio of starch to water in the waxy corn starch is 1: 30; the initiator is 80mg/L ascorbic acid, and the ascorbic acid is 80 mg; the insecticidal bactericide is 120mg/L matrine, and the matrine is 120 mg; the warning color is 20% of iron oxide yellow, and the iron oxide yellow is 200 g; the water is 1L of distilled water; 10ml of the coating agent is used for coating 100g of yellow rose non-heading Chinese cabbage seeds.

2. A preparation method of a natural environment-friendly coating agent for non-heading Chinese cabbage seeds is characterized by comprising the following steps:

step one, weighing 120mg of matrine and 80mg of ascorbic acid to prepare a matrine solution with the concentration of 120mg/L and an ascorbic acid solution with the concentration of 80 mg/L;

step two, dissolving 3.3% of waxy corn starch in the solution obtained in the step one, and fully stirring to fully dissolve the waxy corn starch, wherein the waxy corn starch is 33.3g, and the mass ratio of starch to water in the waxy corn starch is 1: 30;

step three, dissolving 20% of iron oxide yellow in the solution obtained in the step two, and fully stirring to uniformly distribute the iron oxide yellow in the starch solution, wherein the weight of the iron oxide yellow is 200 g;

and step four, coating the yellow rose non-heading Chinese cabbage seeds by using a seed coating machine according to the seed ratio of 1: 10.