CN109769827A - Improve the seed coat agent and preparation method thereof of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage - Google Patents
Improve the seed coat agent and preparation method thereof of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage Download PDFInfo
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- CN109769827A CN109769827A CN201910064791.7A CN201910064791A CN109769827A CN 109769827 A CN109769827 A CN 109769827A CN 201910064791 A CN201910064791 A CN 201910064791A CN 109769827 A CN109769827 A CN 109769827A
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Abstract
The invention discloses the seed coat agents for improving the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage, based on parts by weight, including 25-40 parts of carboxin, 5-6 parts of fludioxonil, 0.2-0.4 parts of plant growth regulator, 7-9 parts of di-isooctyl sulfonate, 7-9 parts of polycarboxylate, 13-15 parts of phosphate, 18-20 parts of ethylene glycol, 0.5-1.5 parts of aluminium-magnesium silicate, 8-10 parts of zinc sulfate, 0.5-0.6 parts of ammonium dihydrogen phosphate, 0.5-0.6 parts of fulvic acid, 8-10 parts of dimethyl silicone polymer, 10-12 parts of organosilicon modified crylic acid resin, 2-3 parts of preservative, 360-380 parts of water.Seed coat agent of the present invention has the multiple functions such as diseases prevention is cold-resistant, solves that cotton emergence survival rate of seedlings is low and the problems such as heavier occurs for seedling stage root-rot class disease, and non-hazardous to environment, using conveniently, safely, can reduce Pesticide use amount, realize the purpose of hidden application.
Description
Technical field
The present invention relates to raising seedling of cotton technical field, more particularly to a kind of raising cotton in seedling stage is low temperature resistant to be damaged to plants caused by sudden drop in temperature
Seed coat agent of ability and preparation method thereof.
Background technique
Cotton is Malvaceae cotton, originates in subtropical zone, happiness temperature, is one of more main crops, usually in 4
Moon sowing.However due to various regions climate difference, when sowing, temperature is low is easy to happen rotten seed, rotten bud, aggravates disease, in turn results in scarce
Seedling breaks ridge.
Sead coating agent for cotton seeds can prevent and treat cotton seed surface germ, soil-borne disease, seed-borne disease, improve germination rate of cotton seed and emergence
Rate, but there are problems for existing sead coating agent for cotton seeds, and such as: broad spectrum activity is strong, specific aim is weak;Preventive effect and safety tend not to simultaneous
?;Easily with rain water runoff after seed dressing, preventive effect is unstable, and the lasting period is short;It has a single function, a kind of seed coat agent is used only can not be simultaneously
Solve various problems, typically without can diseases prevention promotion grows, resists the seed coat agents of the adverse environmental factors such as low temperature again.
Therefore it provides one kind is safe and efficient, function is more, preventive effect stablizes lasting seed coat agent becomes those skilled in the art urgently
Technical problem to be solved.
Summary of the invention
In view of this, the present invention provides the seed coat agent and preparation method thereof for improving the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage,
Seed coat agent Rhizoctonia solani, the quasi- wheel branch fusarium being prepared have significant inhibiting effect, and can be improved cotton
Seed and seedling low temperature tolerance ability promote Developmental of Cotton, and use is safe, and preventive effect is stablized lasting.
To achieve the goals above, the present invention adopts the following technical scheme:
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including 25-40 parts of carboxin, it coughs up
5-6 parts of bacterium nitrile, 0.2-0.4 parts of plant growth regulator, 7-9 parts of di-isooctyl sulfonate, 7-9 parts of polycarboxylate, phosphate 13-
15 parts, 18-20 parts of ethylene glycol, 0.5-1.5 parts of aluminium-magnesium silicate, 8-10 parts of zinc sulfate, 0.5-0.6 parts of ammonium dihydrogen phosphate, fulvic acid
0.5-0.6 parts, 8-10 parts of dimethyl silicone polymer, 10-12 parts of organosilicon modified crylic acid resin, 2-3 parts of preservative and distillation
360-380 parts of water.
Preferably, carboxin is 35-40 parts in seed coat agent ingredient.
Preferably, preservative is one or more of potassium sorbate, sodium benzoate, Kathon CG.
It is further preferred that preservative is potassium sorbate.
Preferably, plant growth regulator is one or more of brassin lactones, gibberellin, compound sodium nitrophenolate, alar-85.
It is further preferred that plant growth regulator is brassin lactones.
The preparation method for improving the seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage, includes the following steps:
(1) first plant growth regulator is dissolved in distilled water, then dissolves in di-isooctyl sulfonate, polycarboxylate, phosphoric acid
Carboxin is added after mixing in ester, fludioxonil stirs evenly;
(2) zinc sulfate, ammonium dihydrogen phosphate, fulvic acid, ethylene glycol, dimethyl silicone polymer are sequentially added to mix, is poured into
In reaction kettle, opens sand mill and 40-50min is sanded;
(3) organosilicon modified crylic acid resin, preservative is added, material in reaction kettle is transferred to high-speed shearing emulsion machine
High speed shears 15-20min, and aluminium-magnesium silicate is added, and adjusts pH5-7 using acid-base modifier, detects particle size, 5 μ of D90 <
M is to get finished product.
Finished appearance of the present invention is flowable, the slightly sticky liquid of milky, and viscosity complies with standard, and pH shows slightly acid, is hanged
Buoyancy is good, and expulsion rate is low, and cold storage, heat storage stability are good, and film formation time is short.
The method for carrying out seed pelleting using the seed coat agent of the above-mentioned low temperature resistant ability of damaging to plants caused by sudden drop in temperature of raising cotton in seedling stage, by seed coat agent
It is mixed with cotton seeds by 1:100-150 weight ratio, naturally dry is under cool environment to get cotton capsuled seed.
Seed coat agent of the present invention compounds carboxin, fludioxonil, is aided with ammonium dihydrogen phosphate, fulvic acid synergy, promotes cotton
Seedling root growth increases Growth of seedling and anti-seismic design, improves sprout term disease preventive effect.Seed coat agent of the present invention is to environment without danger
Evil, using conveniently, safely, can reduce the usage amount of pesticide and fertilizer, realize hidden application.
Detailed description of the invention
Fig. 1 show Rhizoctonia solani Kuhn colonial morphology;
Fig. 2 show Rhizoctonia Solani form;
Fig. 3 show quasi- wheel branch fusarium colonial morphology;
Fig. 4 show quasi- wheel branch Fusarium filament shapes;
Fig. 5 show Rhizoctonia solani Kuhn toxicity test result;
Fig. 6 show quasi- wheel branch fusarium toxicity test result;
Fig. 7 show 1 low temperature stress Seed germination result of embodiment;
Fig. 8 show control group low temperature stress Seed germination result;
Fig. 9 show emergence experimental result.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 37.8g, cough up bacterium
Nitrile 5.2g, brassin lactones 0.3g, Aerosol OT 8g, sodium polycarboxylate 8g, castor oil polyoxyethylene ether phosphoric acid
Ester 14g, ethylene glycol 20g, aluminium-magnesium silicate 1g, zinc sulfate 10g, ammonium dihydrogen phosphate 0.5g, fulvic acid 0.5g, dimethyl silicone polymer
8g, organosilicon modified crylic acid resin 10g, potassium sorbate 2.5g, excess water 374.5g.
Above-mentioned seed coat agent the preparation method is as follows:
Brassin lactones are dissolved in distilled water, then dissolves in Aerosol OT, sodium polycarboxylate, castor oil and gathers
Carboxin is added after mixing in ethylene oxide ether phosphate, fludioxonil stirs evenly;Sequentially add zinc sulfate, ammonium dihydrogen phosphate, Huang
Rotten acid, ethylene glycol, dimethyl silicone polymer mix, and pour into reaction kettle, open sand mill and 45min is sanded;Organosilicon is added to change
Property acrylic resin, potassium sorbate, by material in reaction kettle be transferred to high-speed shearing emulsion machine high speed shearing 20min, be added
Aluminium-magnesium silicate adjusts pH5-7 using acid-base modifier, detects particle size, D905 μm of < to get finished product.
Embodiment 2
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 30g, fludioxonil
5.8g, brassin lactones 0.2g, diisooctyl succinate sodium sulfonate 9g, sodium polycarboxylate 9g, castor oil polyoxyethylene ether phosphate
15g, ethylene glycol 19g, aluminium-magnesium silicate 1.5g, zinc sulfate 9g, ammonium dihydrogen phosphate 0.6g, fulvic acid 0.6g, dimethyl silicone polymer
9g, organosilicon modified crylic acid resin 18g, potassium sorbate 2.5g, excess water 370.8g.
The preparation method of seed coat agent such as embodiment 1.
Embodiment 3
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 37.8g, cough up bacterium
Nitrile 5.5g, brassin lactones 0.4g, Aerosol OT 9g, sodium polycarboxylate 7g, castor oil polyoxyethylene ether phosphoric acid
Ester 13g, ethylene glycol 18g, aluminium-magnesium silicate 0.6g, zinc sulfate 8g, ammonium dihydrogen phosphate 0.5g, fulvic acid 0.5g, polydimethylsiloxanes
Alkane 10g, organosilicon modified crylic acid resin 12g, potassium sorbate 3g, excess water 374.7g.
The preparation method of seed coat agent such as embodiment 1.
Comparative example 1
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 20g, fludioxonil
5.2g, brassin lactones 0.3g, Aerosol OT 8g, sodium polycarboxylate 8g, castor oil polyoxyethylene ether phosphate
14g, ethylene glycol 20g, aluminium-magnesium silicate 1g, zinc sulfate 10g, ammonium dihydrogen phosphate 0.5g, fulvic acid 0.5g, dimethyl silicone polymer
8g, organosilicon modified crylic acid resin 10g, potassium sorbate 2.5g, excess water 374.5g.
The preparation method of seed coat agent such as embodiment 1.
Comparative example 2
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 37.8g, Fu Mei
Double 5.2g, brassin lactones 0.3g, Aerosol OT 8g, sodium polycarboxylate 8g, castor oil polyoxyethylene ether phosphoric acid
Ester 14g, ethylene glycol 20g, aluminium-magnesium silicate 1g, zinc sulfate 10g, ammonium dihydrogen phosphate 0.5g, fulvic acid 0.5g, dimethyl silicone polymer
8g, organosilicon modified crylic acid resin 10g, potassium sorbate 2.5g, excess water 374.5g.
The preparation method of seed coat agent such as embodiment 1.
Comparative example 3
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 37.8g, cough up bacterium
Nitrile 5.2g, brassin lactones 0.3g, Aerosol OT 8g, sodium polycarboxylate 8g, castor oil polyoxyethylene ether phosphoric acid
Ester 14g, ethylene glycol 20g, aluminium-magnesium silicate 1g, zinc sulfate 10g, ammonium dihydrogen phosphate 0.5g, dimethyl silicone polymer 8g, organosilicon change
Property acrylic resin 10g, potassium sorbate 2.5g, excess water 374.5g.
The preparation method of seed coat agent such as embodiment 1.
Comparative example 4
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 37.8g, cough up bacterium
Nitrile 5.2g, brassin lactones 0.3g, Aerosol OT 8g, sodium polycarboxylate 8g, castor oil polyoxyethylene ether phosphoric acid
Ester 14g, ethylene glycol 20g, aluminium-magnesium silicate 1g, zinc sulfate 10g, ammonium dihydrogen phosphate 0.5g, fulvic acid 0.5g, dimethyl silicone polymer
2g, mass fraction 1% chitosan aqueous solution 10g, potassium sorbate 2.5g, excess water 374.5g.
The preparation method of seed coat agent such as embodiment 1.
Comparative example 5
The seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage is improved, based on parts by weight, including carboxin 37.8g, cough up bacterium
Nitrile 5.2g, brassin lactones 0.3g, Aerosol OT 8g, sodium polycarboxylate 8g, castor oil polyoxyethylene ether phosphoric acid
Ester 14g, ethylene glycol 20g, aluminium-magnesium silicate 1g, zinc sulfate 10g, ammonium dihydrogen phosphate 0.5g, fulvic acid 0.5g, dimethyl silicone polymer
8g, organosilicon modified crylic acid resin 10g, potassium sorbate 2.5g, excess water 374.5g.
Above-mentioned seed coat agent the preparation method is as follows:
By carboxin, fludioxonil, brassin lactones, Aerosol OT, sodium polycarboxylate, castor oil polyoxy second
Alkene ether phosphate, zinc sulfate, ammonium dihydrogen phosphate, fulvic acid, ethylene glycol, dimethyl silicone polymer mixing are added in distilled water,
It pours into reaction kettle, opens sand mill and 45min is sanded;Styrene acrylic emulsion polymer, potassium sorbate is added, by reaction kettle
Middle material is transferred to high-speed shearing emulsion machine high speed shearing 20min, and aluminium-magnesium silicate is added, and adjusts pH5- using acid-base modifier
7, particle size is detected, 5 μm of D90 < to get finished product.
To embodiment 1-3, the seed coat agent performance indicator in comparative example 1-5 is evaluated, evaluation method is as follows:
Heat storage stability measuring method: it is measured according to GB/T19136-2003.
Cold storage 4stability determination: it is measured according to GB/T19137-2003.
Viscosity measurement: it is measured using NDJ-5S digital display viscosimeter.
The measuring method of suspensibility: it is measured according to GB/T14825-2006.
The results are shown in Table 1.
The performance indicator testing result of 1 seed coat agent of table
The dyeing of 0.05% basic rhodamine is added in the seed coat agent in embodiment 1-3, comparative example 1-5, by pharmacopoeia weight
It is coated than six group seeds of the 1:100 to identical quantity, under cool environment after natural drying, 1h is impregnated in water, expose kind
Sub-color is to fall off, and calculates separately every group of seed coat agent expulsion rate, as shown in table 2.
The detection of 2 seed coat agent expulsion rate of table
Using embodiment 1-3, the seed coat agent in comparative example 1-5 carries out bacteriostatic experiment.Experimental procedure is as follows:
Strains tested (Rhizoctonia solani Kuhn, quasi- wheel branch fusarium) is activated into (Fig. 1-4) on PSA plating medium, then
It is inoculated in PSA slant medium, 25 DEG C of constant temperature incubation 3d;3mL sterile water is added into test tube, it is spare that bacteria suspension is made in jog.
PSA culture medium is dispensed into culture dish (diameter 9cm, every culture dish 10mL) by sterile working, plate training is made
Base is supported, the above-mentioned bacteria suspension spread plate of 100 μ L is drawn.After to be dried, stacked in culture dish central heavy and set 2 diameter 1cm
Sterilize circular filter paper piece, 50 μ L seed coat agents is added dropwise in filter paper center, each processing is repeated 3 times, the processing of sterile water is added
As control (CK), it is put into 25 DEG C of incubators and cultivates.
After control treatment bacterium colony covers with culture dish, it is straight that each seed coat agent processing group inhibition zone is measured using crossing method
Diameter counts antibacterial radius, calculates bacteriostasis rate, and test result is as shown in table 3.
Table 3 compares the inhibition zone size of quasi- wheel branch fusarium and Rhizoctonia solani Kuhn
Note: 1. bacteriostasis rate is that embodiment and comparative example compare the inhibition zone size of quasi- wheel branch fusarium, 2. bacteriostasis rate is
Embodiment and the inhibition zone size of comparative example Rhizoctonia solani compare.
Further, embodiment 1-3 seed coat agent is diluted into 7 gradients, draws various concentration gradient seed coat agent 1mL and is added to
The sterilizing of 49mL is cooled in 45 DEG C of PSA, is poured into 3 sterilizes culture dishes after mixing, pastille culture medium is made, in ultra-clean work
Make to dispense on platform into culture dish.After culture medium solidification and dry tack free, diameter 5mm bacterium is beaten with the identical punch of diameter
Cake is respectively placed in each processing culture medium middle position, and mycelia is downwards.It is handled so that seed coat agent is not added as control (CK),
After 25 DEG C of constant temperature incubation 72h, using crossing method measurement it is each repeat colony diameter, count antibacterial radius and control bacterium colony with
The difference of each processing colony radius, calculates inhibiting rate.
Bacterium colony inhibiting rate=(control colony radius-processing colony radius)/control colony radius × 100%.
EC50Statistical method: being independent variable according to the logarithm of seed coat agent concentration (mL/L), and the probability of inhibiting rate is because becoming
Amount, establishes virulence regression equation with SPSS20.0, finds out each seed coat agent half-maximal effect concentration EC50, as a result such as table 4, Fig. 5-6
It is shown.
4 Rhizoctonia solani Kuhn of table, quasi- wheel branch fusarium toxicity test result
Health cotton seeds full, of uniform size are selected, cotton variety is that new land is No. 82 early.It is right using embodiment 1-3
Seed coat agent in ratio 1-5 is coated (coating ratio is according to pharmacopoeia weight ratio 1:100) cotton seeds, and it is real to carry out germination
It tests.Experimental procedure is as follows:
Low temperature stress Seed germination
Each processing seed sowing is taken to be respectively put into 10 DEG C, 15 DEG C, 20 DEG C of illumination in plastic nutritional box (matrix is fine sand)
Incubator, intensity of illumination 12000lx, photoperiod are 14h/10h (day night), relative humidity 70-75%.It is measured after sowing 4d
Its germination percentage, and carry out the control group of uncoated processing.Experimental result is as shown in table 5.
The influence that 5 low temperature stress of table emerges to cotton
Further, 7d is sowed, embodiment 1 and control group germination percentage compare as shown in Figure 7,8.
Growth rate measures after seed sprouting
The full seed of the same size of health is chosen, processing is coated by pharmacopoeia mass ratio 1:100, is seeded in after drying in the shade
Plastics germinate in box.Each processing seed sowing is taken to be put into 25 DEG C of illumination boxs in plastic nutritional box (matrix is fine sand), (L:
D=12:12, (27 ± 1 DEG C), 70-75%RH), every processing is repeated 3 times.4d measures its germinating energy, and 10d counts seed hair
Bud number, and calculate its emergence rate, each processing in the 14th day take 10 plants of samples at random, measure its plant height, stem thickness, root long, fresh and dried
Equal economical characters again.
Experimental result such as Fig. 9, shown in table 6.
6 different disposal seed coat agent of table is emerged to cotton and the influence of growth of seedling
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (5)
1. improving the seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage, which is characterized in that based on parts by weight, including carboxin
25-40 parts, 5-6 parts of fludioxonil, 0.2-0.4 parts of plant growth regulator, 7-9 parts of di-isooctyl sulfonate, polycarboxylate 7-9
Part, 13-15 parts of phosphate, 18-20 parts of ethylene glycol, 0.5-1.5 parts of aluminium-magnesium silicate, 8-10 parts of zinc sulfate, ammonium dihydrogen phosphate 0.5-
0.6 part, 0.5-0.6 parts of fulvic acid, 8-10 parts of dimethyl silicone polymer, 10-12 parts of organosilicon modified crylic acid resin, preservative
2-3 parts and distilled water 360-380 parts.
2. the seed coat agent according to claim 1 for improving the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage, which is characterized in that described anti-
Rotten agent is one or more of potassium sorbate, sodium benzoate, Kathon CG.
3. the seed coat agent according to claim 1 for improving the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage, which is characterized in that the plant
Object growth regulator is one or more of brassin lactones, gibberellin, compound sodium nitrophenolate, alar-85.
4. improving the preparation method of the seed coat agent of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage, which comprises the steps of:
(1) first plant growth regulator is dissolved in distilled water, then dissolves in di-isooctyl sulfonate, polycarboxylate, phosphate, mixed
Carboxin is added after conjunction, fludioxonil stirs evenly;
(2) zinc sulfate, ammonium dihydrogen phosphate, fulvic acid, ethylene glycol, dimethyl silicone polymer are sequentially added to mix, pours into reaction
In kettle, opens sand mill and 40-50min is sanded;
(3) organosilicon modified crylic acid resin, preservative is added, material in reaction kettle is transferred in high-speed shearing emulsion machine high
Speed shearing 15-20min, is added aluminium-magnesium silicate, adjusts pH5-7 using acid-base modifier, detects particle size, D905 μm of < to get
Finished product.
5. carrying out seed using the seed coat agent of the raising low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage of any of claims 1-4
The method of coating, which is characterized in that seed coat agent is mixed with cotton seeds by 1:100-150 weight ratio, it is natural under cool environment
It dries to get cotton capsuled seed.
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CN111631224A (en) * | 2020-06-18 | 2020-09-08 | 江苏徐淮地区淮阴农业科学研究所 | Rice seed dressing agent and preparation method and application thereof |
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Application publication date: 20190521 |