CN111631224A - Rice seed dressing agent and preparation method and application thereof - Google Patents

Rice seed dressing agent and preparation method and application thereof Download PDF

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Publication number
CN111631224A
CN111631224A CN202010557908.8A CN202010557908A CN111631224A CN 111631224 A CN111631224 A CN 111631224A CN 202010557908 A CN202010557908 A CN 202010557908A CN 111631224 A CN111631224 A CN 111631224A
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seed dressing
dressing agent
bactericide
rice
parts
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CN111631224B (en
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顾大路
王伟中
钱新民
孙爱侠
杨文飞
杜小凤
文廷刚
贾艳艳
吴雪芬
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JIANGSU XUHUAI DISTRICT HUAIYIN AGRICULTURAL SCIENCE RESEARCH INSTITUTE
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JIANGSU XUHUAI DISTRICT HUAIYIN AGRICULTURAL SCIENCE RESEARCH INSTITUTE
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Priority to PCT/CN2021/100925 priority patent/WO2021254485A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
    • A01N41/10Sulfones; Sulfoxides
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16Heavy metals; Compounds thereof

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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
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  • Chemical & Material Sciences (AREA)
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Abstract

The invention belongs to the technical field of seed dressing agents, and particularly relates to a rice seed dressing agent as well as a preparation method and application thereof. The rice seed dressing agent comprises the following components in parts by mass: 1-4 parts of dimethyl sulfoxide, 1-4 parts of zinc sulfate, 0.01-0.04 part of thidiazuron, 5-8 parts of betaine, 0.5-2 parts of a bactericide and 31.96-42.49 parts of attapulgite. The seed dressing agent of the invention is compounded by thidiazuron, dimethyl sulfoxide, zinc sulfate, betaine and bactericide, which not only can activate the activity of active substances and enzymes in rice bodies, resist the influence of adverse circumstances and enhance the frost resistance of seedlings; but also can improve the germination vigor, promote the root system to be developed, improve the activity of the root system, improve the disease prevention and resistance of the seedlings and promote the seedlings to grow robustly.

Description

Rice seed dressing agent and preparation method and application thereof
Technical Field
The invention belongs to the technical field of seed dressing agents, and particularly relates to a rice seed dressing agent as well as a preparation method and application thereof.
Background
Rice is one of three major food crops in China. Along with the continuous change of the climate environment, the frequent outbreak of extreme weather in the world, and the natural disasters such as high temperature, drought, low temperature, waterlogging, salt and alkali are more and more, so that the germination rate of rice is low, the growth of seedlings is slow, the root system is dysplasia, leaves are stiff but not grown, the disease resistance is reduced, the disease is easy to be infected by germs to cause diseases, and the rice production is seriously influenced.
At present, some seed dressing agents are researched and applied on crops to solve the problems, but the seed dressing agents still belong to an initial stage, most of research related to seed dressing of the seed dressing agents focuses on insect prevention and disease prevention, or research on the influence of the seed dressing agents on the yield and the quality of rice seedlings, and research on the influence of the seed dressing agents on the frost resistance of the rice seedlings, the activity of the root systems of the rice seedlings and antioxidant protective enzymes is few.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provides a rice seed dressing agent and a preparation method and application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a rice seed dressing agent which comprises the following components in parts by mass: 1-4 parts of dimethyl sulfoxide, 1-4 parts of zinc sulfate, 0.01-0.04 part of thidiazuron, 5-8 parts of betaine, 0.5-2 parts of a bactericide and 31.96-42.49 parts of attapulgite.
Preferably, the fungicide comprises azoxystrobin and/or hymexazol.
Preferably, the bactericide is provided in the form of a solution, and the kind of the solvent is determined according to the kind of the bactericide;
when the bactericide is azoxystrobin or a mixture of azoxystrobin and hymexazol, the solvent is an organic solvent;
when the bactericide is hymexazol, the solvent is water.
Preferably, the organic solvent comprises methanol and/or ethyl acetate.
Preferably, the volume of the solvent to the mass ratio of the bactericide depends on the kind of the bactericide;
when the bactericide is azoxystrobin or a mixture of azoxystrobin and hymexazol, the mass ratio of the volume of the solvent to the bactericide is 1g: 10-30 ml;
when the bactericide is hymexazol, the mass ratio of the volume of the solvent to the bactericide is 1g: 40-60 ml.
The invention provides a preparation method of the rice seed dressing agent in the scheme, which comprises the following steps:
dissolving the bactericide in a solvent to obtain a bactericide solution;
mixing the bactericide solution with attapulgite, and drying to obtain a primary seed dressing agent;
and mixing the primary seed dressing agent with dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine, and drying to obtain the rice seed dressing agent.
Preferably, the mixing mode of the primary seed dressing agent, dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine is as follows: and sequentially adding dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine into the primary seed dressing agent to obtain the rice seed dressing agent.
The invention provides the application of the seed dressing agent in the scheme or the rice seed dressing agent obtained by the preparation method in disease prevention, stress resistance and growth promotion of rice.
Preferably, in the rice seeding period, the seed dressing agent is mixed with water to be dressed on rice seeds, and the seeds are sowed in the shade; the mass ratio of the seed dressing agent to the water to the rice seeds is 1: 6-10: 200.
Has the advantages that:
the invention provides a rice seed dressing agent which comprises the following components in parts by mass: 1-4 parts of dimethyl sulfoxide, 1-4 parts of zinc sulfate, 0.01-0.04 part of thidiazuron, 5-8 parts of betaine, 0.5-2 parts of a bactericide and 32-42 parts of attapulgite. The seed dressing agent of the invention not only activates the activity of active substances and enzymes in rice seeds and enhances the frost resistance of seedlings, but also has significant synergy for improving the activity of seedling roots by compounding the thidiazuron, the dimethyl sulfoxide, the zinc sulfate, the betaine and the bactericide. The results of the embodiment show that the seed dressing agent provided by the invention obviously increases the activity of rice antioxidant enzyme, so that the POD is increased by 7.98-11.11%, the SOD is increased by 12.98-14.74%, the CAT is increased by 12.34-14.27%, and the MDA content is reduced by 16.01-21.06%, thereby further reducing the damage of adverse environmental stress on plant cells, especially the damage of low-temperature and low-seedling; moreover, the seed dressing agent provided by the invention obviously improves the activity of the root system of the seedling, and improves the activity of the root system by 3.28-11.08%.
Furthermore, the seed dressing agent of the invention combines the thidiazuron, the dimethyl sulfoxide, the zinc sulfate, the betaine and the bactericide for use, thereby not only obviously improving the germination potential and the emergence rate, having obvious synergy on the growth and development of seedlings and the root growth promotion, but also improving the disease resistance and prevention effects on the bakanae disease of rice.
Detailed Description
The invention provides a rice seed dressing agent which comprises the following components in parts by mass: 1-4 parts of dimethyl sulfoxide, 1-4 parts of zinc sulfate, 0.01-0.04 part of thidiazuron, 5-8 parts of betaine, 0.5-2 parts of a bactericide and 31.96-42.49 parts of attapulgite. In the present invention, the total mass of each component is preferably 50 parts.
Unless otherwise specified, the present invention does not require any particular source for each component of the rice seed dressing agent, and any commercially available product known to those skilled in the art may be used.
The rice seed dressing agent provided by the invention comprises 1-4 parts by mass of dimethyl sulfoxide, more preferably 1-2 parts by mass, and most preferably 1 part by mass. The dimethyl sulfoxide provided by the invention not only activates the activity of active substances and enzymes in rice seeds, but also improves the root activity of seedlings, thereby promoting the growth and development of the seedlings.
The rice seed dressing agent provided by the invention comprises 1-4 parts of zinc sulfate, preferably 1-2 parts of zinc sulfate, and most preferably 1 part of dimethyl sulfoxide. The zinc sulfate of the invention has promotion effect on the vitality and the enzyme activity of seedling roots, so that the activation characteristic of the dimethyl sulfoxide is exerted efficiently.
The rice seed dressing agent provided by the invention comprises 0.01-0.4 part of thidiazuron by mass parts of dimethyl sulfoxide, preferably 0.01-0.02 part of thidiazuron by mass parts of dimethyl sulfoxide, and most preferably 0.01 part of thidiazuron by mass parts of dimethyl sulfoxide. The thidiazuron and the dimethyl sulfoxide have synergistic effect, so that the activity of active substances and enzymes in rice seeds is activated, the frost resistance of seedlings is enhanced, and the root activity of the seedlings is improved.
The rice seed dressing agent provided by the invention comprises 5-8 parts of betaine, preferably 5-7 parts of betaine, and most preferably 5 parts of dimethyl sulfoxide. The betaine and the thidiazuron have synergistic effect, so that the activity of active substances and enzymes in rice seeds is activated, the frost resistance of seedlings is enhanced, and the root activity of the seedlings is improved.
The rice seed dressing agent provided by the invention comprises 32-42 parts of attapulgite, more preferably 31.96-42.49 parts, and most preferably 42 parts by mass of the dimethyl sulfoxide. The attapulgite can adsorb other components to achieve better and uniform mixing effect, can slowly release the other components on the surface of seeds to prolong the action time of the other components, and further better plays the anti-adversity and growth promoting effect of the rice seed dressing agent.
The rice seed dressing agent provided by the invention comprises 0.5-2 parts of bactericide, more preferably 0.5-1 part, and most preferably 0.5 part by mass based on the mass parts of dimethyl sulfoxide. The fungicide of the present invention preferably comprises azoxystrobin and/or hymexazol. The bactericide of the invention can improve the disease resistance and prevention effect on rice bakanae disease under the action of thidiazuron and betaine.
In the invention, the bactericide is preferably provided in a solution form, and the bactericide provided in the invention in the solution form can be better adsorbed on the attapulgite, so that the bactericide can be slowly released on the surface of the seed by utilizing the characteristics of the attapulgite, and the action time of the medicine is prolonged.
In the present invention, the kind of the solvent is preferably determined depending on the kind of the bactericide;
when the bactericide is azoxystrobin or a mixture of azoxystrobin and hymexazol, the solvent is preferably an organic solvent; the organic solvent preferably comprises methanol and/or ethyl acetate.
When the bactericide is hymexazol, the solvent is preferably water.
In the present invention, the volume of the solvent to the mass ratio of the bactericide is preferably determined depending on the kind of the bactericide;
when the bactericide is azoxystrobin or a mixture of azoxystrobin and hymexazol, the mass ratio of the volume of the solvent to the bactericide is preferably 1g: 10-30 ml, more preferably 1g: 15-25 ml, and most preferably 1g:20 ml;
when the bactericide is hymexazol, the mass ratio of the volume of the solvent to the bactericide is preferably 1g: 40-60 ml, more preferably 1g: 45-55 ml, and most preferably 1g:50 ml.
The invention also provides a preparation method of the rice seed dressing agent in the scheme, which comprises the following steps:
dissolving the bactericide in a solvent to obtain a bactericide solution;
mixing the bactericide solution with attapulgite, and drying to obtain a primary seed dressing agent;
and mixing the primary seed dressing agent with dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine, and drying to obtain the rice seed dressing agent.
The bactericide is dissolved in a solvent to obtain a bactericide solution. The invention has no special requirements on the dissolving mode, and the method is known by the technical personnel in the field; in the embodiment of the present invention, the method is specifically a stirring dissolution method.
After the bactericide solution is obtained, the bactericide solution is mixed with the attapulgite and dried to obtain the primary seed dressing agent. In the present invention, the drying mode is preferably drying, and the drying temperature is preferably 35 ℃. The invention firstly dissolves the bactericide in the solution and mixes the bactericide with the attapulgite, and utilizes the characteristic that the bactericide is not easy to be adsorbed on the attapulgite, but the bactericide solution is easy to be adsorbed on the attapulgite; according to the characteristics that the adsorbing behaviors of the bactericide solution, dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine are different on the attapulgite, the bactericide is mixed with the attapulgite firstly, and then the rice seed dressing agent with the bactericide solution uniformly adsorbed on the attapulgite is obtained.
In the invention, the mixing mode of the primary seed dressing agent, dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine is preferably as follows: and sequentially adding dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine into the primary seed dressing agent, and drying to obtain the rice seed dressing agent.
According to the characteristics that dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine are different in adsorption behaviors on attapulgite, the dimethyl sulfoxide, the zinc sulfate, the thidiazuron and the betaine are sequentially added into the first mixture and fully stirred each time, and then the rice seed dressing agent with the effective components uniformly mixed and adsorbed on the attapulgite is obtained.
The invention also provides the application of the seed dressing agent in the scheme or the rice seed dressing agent obtained by the preparation method in disease prevention, stress resistance and growth promotion of rice.
In the invention, preferably, in the rice seeding period, the seed dressing agent is mixed with water to be dressed on rice seeds, and the seeds are sowed in the shade; the mass ratio of the seed dressing agent to the water to the rice seeds is preferably 1: 6-10: 200, and more preferably 1:8: 200.
To further illustrate the present invention, the following examples are provided to describe in detail the rice seed dressing agent and the preparation method and application thereof, but they should not be construed as limiting the scope of the present invention.
Example 1
A rice seed dressing agent comprises the following components: 1g of dimethyl sulfoxide, 1g of zinc sulfate, 0.01g of thidiazuron, 5g of betaine, 0.5g of azoxystrobin and 42.49g of attapulgite.
The preparation of the rice seed dressing agent comprises the following steps:
measuring azoxystrobin according to a ratio, and dissolving the azoxystrobin in methanol (the mass-volume ratio of azoxystrobin to methanol is 1g:20ml) to obtain a bactericide solution;
mixing the bactericide solution with attapulgite in a proportion, fully stirring, and drying at 35 ℃ to obtain a primary seed dressing agent;
and sequentially adding dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine in proportion into the primary seed dressing agent, fully and uniformly stirring each time, and drying at 35 ℃ to obtain the rice seed dressing agent.
Example 2
A rice seed dressing agent comprises the following components: 2g of dimethyl sulfoxide, 2g of zinc sulfate, 0.02g of thidiazuron, 6g of betaine, 1g of azoxystrobin and 38.08g of attapulgite.
The procedure for the preparation is the same as in example 1.
Example 3
A rice seed dressing agent comprises the following components: 3g of dimethyl sulfoxide, 3g of zinc sulfate, 0.03g of thidiazuron, 7g of betaine, 1.5g of azoxystrobin and 35.47g of attapulgite.
The procedure for the preparation is the same as in example 1.
Example 4
A rice seed dressing agent comprises the following components: 4g of dimethyl sulfoxide, 4g of zinc sulfate, 0.04g of thidiazuron, 8g of betaine, 2g of azoxystrobin and 31.96g of attapulgite.
The procedure for the preparation is the same as in example 1.
Example 5
A seed dressing was prepared as in example 1, except that azoxystrobin was replaced by hymexazol and the solvent was water (the mass to volume ratio of hymexazol to water was 1g:50 ml).
Example 6
A seed dressing was prepared as in example 1, except that azoxystrobin was replaced with 0.25g azoxystrobin and 0.25g hymexazol.
Application example 1
Potting test of rice seed dressing agent
The invention adopts Nanjing 9108 rice variety to perform a pot culture test. The potting test was carried out in 2018, 6 and 14 days in a laboratory of the farm institute of Huaian city, and the test treatment was designed as follows:
the rice seed dressing agent is divided into seven groups A to F, wherein 50g of the rice seed dressing agent prepared in the example 1 is adopted in the group A; b: 1g of dimethyl sulfoxide; c: 1g of zinc sulfate; d: thidiazuron 0.01 g; e: 5g of betaine; f: control (clear water). Each group is respectively mixed with 10Kg of rice seeds, the rice seeds are respectively dissolved and diluted by adding 0.4Kg of water into each treatment seed-mixing agent, then the mixture is mixed, the mixture is naturally dried in the shade, then the treated rice seeds are sown in pot pots (with the upper caliber of 12cm, the lower caliber of 15cm and the height of 15cm) filled with rice field mud, 50 grains are sown in each pot, 15 pot are planted in each group, wherein 6 pot are randomly taken for each treatment, the germination vigor is investigated 3 days after sowing, the emergence rate is investigated 10 days later, and the influence of the rice seed-mixing agent on the germination vigor and the emergence rate of the rice is shown in table 1; and (3) placing the pots into an SHH-250GS artificial climate box when the seedlings are in a three-leaf one-heart period, adjusting the temperature of the artificial climate box to 10 ℃ for low-temperature cold damage treatment for 5 days, then collecting all treated seedlings, mixing and grinding leaves of plants for physiological index determination into powder, and storing in an environment at-80 ℃ for determination.
And (3) measuring the activity of antioxidant enzyme and the content of malonaldehyde: the Malondialdehyde (MDA) content is measured by a thiobarbituric acid (TBA) oxidation method; peroxidase (POD) Activity assay Using guaiacol method, as OD470The variation value of (A) is 0.1/min which is a relative enzyme activity unit U; catalase (CAT) activity was measured by hydrogen peroxide method using OD240The variation value of (A) is 0.1/min which is a relative enzyme activity unit U; the activity of superoxide dismutase (SOD) is determined by adopting nitrotetra chlorideThe azoniazole (NBT) photochemical reduction method takes 50 percent inhibition as 1 enzyme activity unit U, and the influence of the rice seed dressing agent on the rice seedling leaf antioxidant system is shown in a table 2.
Application example 2
Field test of rice seed dressing agent
The Nanjing 9108 rice variety was used for field trials. The field test is carried out in Wangyuan of Huaian city in 2018, 6 months and 15 days, the previous wheat stubble is sticky loam, and the fertility is raised to the upper level. The test treatments were designed as:
the experimental groups were separately dressed with the rice growth dressing prepared in example 1; seed dressing of azoxystrobin; 2, dressing the hymexazol seeds; the control group was dressed with clear water. The test group and the control group were each repeated 3 times, and arranged in random blocks, each treatment area having an area of 50m2. Mixing rice seeds 10Kg each group, dissolving and diluting the rice seeds with each treatment seed-mixing agent 0.4Kg with water, mixing, naturally drying in the shade, rotary tillage and direct seeding at 6 months and 15 days, and sowing at a rate of 10Kg/666.7m2Sowing and field management are consistent with normal field management.
Sampling is carried out at 5 points in the 4-leaf stage of the rice seedling, 100 plants are sampled in each group, and each quality of the rice seedling is determined. Weighing the dry weights of the overground part and the underground part; meanwhile, the root system activity is measured (by adopting a TTC reduction method), the chlorophyll content of functional leaves (two leaves at the top) is measured by using a SPAD-502 type chlorophyll measuring instrument, and the influence of the rice seed dressing agent on the quality of rice seedlings is shown in a table 4.
Investigation of the field bakanae disease rate: 3 points are randomly selected in each treatment area, each point is 1 square meter, the excessive growth and the fineness of a diseased plant are higher than those of a normal healthy plant, the color of the diseased plant is light yellow green, the total seedling number and the number of the seedlings with the bakanae disease of each point are investigated, the diseased plant rate and the prevention and treatment effect are calculated, and the prevention and treatment effect of the rice seed dressing agent on the bakanae disease is shown in Table 3.
Absolute control effect ═ number of diseased plants in blank control zone-number of diseased plants in treatment zone)/number of plants in blank control zone × 100.
Application example 3
The same method as that of application examples 1 and 2 and the same comparative example and control group were set to observe the effects of the seed dressing agents prepared in examples 2 to 6 on the pot experiment and the field experiment.
The influence of the rice seed dressing agent on the germination vigor and the emergence rate of rice is shown in table 1, the influence of the rice seed dressing agent on an antioxidant system of rice seedling leaves is shown in table 2, the control effect of the rice seed dressing agent on bakanae disease is shown in table 3, and the influence of the rice seed dressing agent on the quality of rice seedlings is shown in table 4.
TABLE 1 Effect of different seed dressings on Rice Germination potential and emergence Rate
Treatment of Germination vigor (%) Rate of emergence (%)
Example 1 91.36 98.74
Example 2 91.52 98.23
Example 3 90.89 97.64
Example 4 90.34 97.43
Example 5 88.95 95.32
Examples6 89.16 96.44
Dimethyl sulfoxide 88.27 92.98
Zinc sulfate 77.63 90.33
Thidiazuron 87.15 93.42
Betaine 78.49 90.71
Control 77.54 90.25
TABLE 2 Effect of different seed dressings on the antioxidant System of Rice seedling leaves
Figure BDA0002545003160000081
Figure BDA0002545003160000091
TABLE 3 prevention and treatment of bakanae disease with different seed dressings
Treatment of Disease percentage (%) Control effect (%)
Example 1 0.01 99.5
Example 2 0.02 99.1
Example 3 0.03 98.5
Example 4 0.03 98.4
Example 5 0.04 97.6
Example 6 0.03 98.7
Azoxystrobin 0.05 94.4
Hymexazol 0.06 93.9
Control 1.94
TABLE 4 influence of Rice seed dressing agent on Rice seedling quality
Figure BDA0002545003160000092
As can be seen from Table 1, after the rice seed dressing agent prepared in the embodiments 1-6 of the invention is used for treatment, the germination potential of rice reaches 88.95% -91.52%, which is 11.41% -13.98% higher than that of the control group; the emergence rate reaches 95.32-98.74%, is 5.07-8.49% higher than that of a control, is higher than that of the independent use of dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine, and has obvious synergistic effect.
As can be seen from Table 2, after the rice seed dressing agent prepared in the embodiments 1 to 6 of the invention is used for treatment, compared with a control group, POD is improved by 4.98-11.10%, SOD is improved by 7.30-14.74%, CAT is improved by 9.96-14.27%, MDA content is reduced by 13.89-21.06%, and the POD, SOD, CAT and betaine are all superior to dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine which are used independently. Therefore, the rice seed dressing agent provided by the invention obviously increases the activity of rice antioxidant enzyme, further reduces the damage of adverse environmental stress to plant cells, and enhances the frost resistance of seedlings.
As can be seen from Table 3, the rice seed dressing agent prepared in the embodiments 1 to 6 of the present invention has a rice bakanae disease prevention and treatment effect of 97.6% to 99.5%. Therefore, the rice seed dressing agent provided by the invention improves the disease resistance and prevention effects on the bakanae disease of rice.
As can be seen from table 4, after the rice seed dressing agent prepared in embodiments 1 to 6 of the present invention is used for treatment, the height, stem base width and root number of the seedling are respectively increased by 2.65% to 13.23%, 1.15% to 12.64% and 0.31% to 11.51% compared with the control, the tiller number of a single plant is increased by 33.3% compared with the control, the dry weight of the overground part and the underground part of the seedling is respectively increased by 1.08% to 11.38% and 1.13% to 14.41%, the chlorophyll content of the rice seedling is increased by 0.83% to 14.22%, and the root activity is increased by 0.68% to 8.47%. Therefore, the rice seed dressing agent has obvious synergy on the growth and development of seedlings and the root growth.
According to the embodiment, the rice seed dressing agent provided by the invention not only activates the activity of active substances and enzymes in rice seeds and enhances the frost resistance of seedlings, but also has obvious synergy effect on improving the root activity of seedlings; in addition, the rice seed dressing agent of the invention not only improves the disease-resistant and disease-preventing effects on the bakanae disease of rice, but also has obvious synergy on the growth and development of seedlings and the root growth promotion.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.

Claims (9)

1. The rice seed dressing agent is characterized by comprising the following components in parts by mass: 1-4 parts of dimethyl sulfoxide, 1-4 parts of zinc sulfate, 0.01-0.04 part of thidiazuron, 5-8 parts of betaine, 0.5-2 parts of a bactericide and 31.96-42.49 parts of attapulgite.
2. The rice seed dressing agent according to claim 1, wherein the bactericide comprises azoxystrobin and/or hymexazol.
3. The rice seed dressing agent according to claim 2, wherein the bactericide is provided in the form of a solution, and the kind of the solvent is determined depending on the kind of the bactericide;
when the bactericide is azoxystrobin or a mixture of azoxystrobin and hymexazol, the solvent is an organic solvent;
when the bactericide is hymexazol, the solvent is water.
4. The rice seed dressing agent of claim 3, wherein the organic solvent comprises methanol and/or ethyl acetate.
5. The rice seed dressing agent according to any one of claims 2 to 4, wherein the ratio of the volume of the solvent to the mass of the bactericide is determined according to the kind of the bactericide;
when the bactericide is azoxystrobin or a mixture of azoxystrobin and hymexazol, the mass ratio of the volume of the solvent to the bactericide is 1g: 10-30 ml;
when the bactericide is hymexazol, the mass ratio of the volume of the solvent to the bactericide is 1g: 40-60 ml.
6. A method for preparing a rice seed dressing agent according to any one of claims 1 to 5, comprising the steps of:
dissolving the bactericide in a solvent to obtain a bactericide solution;
mixing the bactericide solution with attapulgite, and drying to obtain a primary seed dressing agent;
and mixing the primary seed dressing agent with dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine, and drying to obtain the rice seed dressing agent.
7. The method of claim 6, wherein the primary seed dressing agent is mixed with dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine in the following manner: and sequentially adding dimethyl sulfoxide, zinc sulfate, thidiazuron and betaine into the primary seed dressing agent, and drying to obtain the rice seed dressing agent.
8. The rice seed dressing agent as set forth in any one of claims 1 to 5 or the rice seed dressing agent obtained by the preparation method as set forth in any one of claims 6 to 7 is applied to disease prevention, stress resistance and growth promotion of rice.
9. The use as claimed in claim 8, wherein, in the rice sowing period, the seed dressing agent is mixed with water and dressed on rice seeds, and the seeds are sowed in the shade; the mass ratio of the seed dressing agent to the water to the rice seeds is 1: 6-10: 200.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021254485A1 (en) * 2020-06-18 2021-12-23 江苏徐淮地区淮阴农业科学研究所 Rice seed dressing agent, and preparation method therefor and use thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115281213B (en) * 2022-08-18 2024-03-29 广西壮族自治区农业科学院 Sugarcane seed stem coating agent applied at normal temperature in large scale and application thereof

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1973630A (en) * 2006-12-08 2007-06-06 浙江大学 Seed coating agent for raising cold resistance of super sweet corn seed
CN101310582A (en) * 2008-03-25 2008-11-26 江苏里下河地区农业科学研究所 Seed coating agent special for direct seeding rice
CN104094931A (en) * 2014-07-31 2014-10-15 辽宁东亚种业有限公司 Seed coating agent for improving cold resistance of maize seeds
CN107306998A (en) * 2017-06-10 2017-11-03 威海温喜生物科技有限公司 A kind of special seed dressing of paddy rice
CN107306941A (en) * 2017-06-21 2017-11-03 深圳千色龙种子健康科技与服务有限公司 Seed dressing and preparation method and application
WO2017208009A1 (en) * 2016-06-02 2017-12-07 Plant Impact Plc Plant treatment composition
CN109180362A (en) * 2018-11-20 2019-01-11 中国农业科学院农业环境与可持续发展研究所 The composition that there is a kind of pair of crop the transfer of degeneration-resistant potential and root cap to promote pressure function
CN109526953A (en) * 2018-12-21 2019-03-29 石家庄博佳特化工有限公司 A kind of low temperature resistant resistance to hard water flies anti-auxiliary agent
CN109769827A (en) * 2019-01-23 2019-05-21 新疆农业科学院核技术生物技术研究所(新疆维吾尔自治区生物技术研究中心) Improve the seed coat agent and preparation method thereof of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage
CN109769848A (en) * 2019-03-11 2019-05-21 李永平 A kind of plant growth regulator composition
CN109907063A (en) * 2019-04-04 2019-06-21 李永平 Plant growth regulator composition containing Thidiazuron

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070149409A1 (en) * 2003-12-29 2007-06-28 Hi-Cap Formulations Ltd. Pesticide formulations with substituted biopolymers and organic polymers for improving residual activity, droplet size, adherence and rainfastness on leaves and reduction in soil leaching
CN107298592A (en) * 2017-06-13 2017-10-27 王学才 Direct seading rice seed dressing
CN108208038A (en) * 2018-02-06 2018-06-29 金华市铁骑士生物科技有限公司 A kind of seed dressing suitable for rice
CN111631224B (en) * 2020-06-18 2021-05-04 江苏徐淮地区淮阴农业科学研究所 Rice seed dressing agent and preparation method and application thereof
AU2020101473A4 (en) * 2020-07-24 2020-09-03 Jiangsu Xuhuai Area Huaiyin Agricultural Science Research Institute Rice conditioner for promoting rice seed germination and preparation method and use thereof

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1973630A (en) * 2006-12-08 2007-06-06 浙江大学 Seed coating agent for raising cold resistance of super sweet corn seed
CN101310582A (en) * 2008-03-25 2008-11-26 江苏里下河地区农业科学研究所 Seed coating agent special for direct seeding rice
CN104094931A (en) * 2014-07-31 2014-10-15 辽宁东亚种业有限公司 Seed coating agent for improving cold resistance of maize seeds
WO2017208009A1 (en) * 2016-06-02 2017-12-07 Plant Impact Plc Plant treatment composition
CN107306998A (en) * 2017-06-10 2017-11-03 威海温喜生物科技有限公司 A kind of special seed dressing of paddy rice
CN107306941A (en) * 2017-06-21 2017-11-03 深圳千色龙种子健康科技与服务有限公司 Seed dressing and preparation method and application
CN109180362A (en) * 2018-11-20 2019-01-11 中国农业科学院农业环境与可持续发展研究所 The composition that there is a kind of pair of crop the transfer of degeneration-resistant potential and root cap to promote pressure function
CN109526953A (en) * 2018-12-21 2019-03-29 石家庄博佳特化工有限公司 A kind of low temperature resistant resistance to hard water flies anti-auxiliary agent
CN109769827A (en) * 2019-01-23 2019-05-21 新疆农业科学院核技术生物技术研究所(新疆维吾尔自治区生物技术研究中心) Improve the seed coat agent and preparation method thereof of the low temperature resistant ability of damaging to plants caused by sudden drop in temperature of cotton in seedling stage
CN109769848A (en) * 2019-03-11 2019-05-21 李永平 A kind of plant growth regulator composition
CN109907063A (en) * 2019-04-04 2019-06-21 李永平 Plant growth regulator composition containing Thidiazuron

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021254485A1 (en) * 2020-06-18 2021-12-23 江苏徐淮地区淮阴农业科学研究所 Rice seed dressing agent, and preparation method therefor and use thereof

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