CN109757489A - A kind of Chinese cabbage seed initiator and its application method - Google Patents

A kind of Chinese cabbage seed initiator and its application method Download PDF

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Publication number
CN109757489A
CN109757489A CN201910167111.4A CN201910167111A CN109757489A CN 109757489 A CN109757489 A CN 109757489A CN 201910167111 A CN201910167111 A CN 201910167111A CN 109757489 A CN109757489 A CN 109757489A
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China
Prior art keywords
chinese cabbage
seed
ascorbic acid
cabbage seed
initiator
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CN201910167111.4A
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Chinese (zh)
Inventor
侯喜林
颜晓艺
刘高峰
张昌伟
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Nanjing Agricultural University
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Nanjing Agricultural University
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Priority to CN201910167111.4A priority Critical patent/CN109757489A/en
Publication of CN109757489A publication Critical patent/CN109757489A/en
Priority to PCT/CN2019/125941 priority patent/WO2020177442A1/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention relates to Seed priming techniques fields, specifically a kind of Chinese cabbage seed initiator, it include: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, fungicide, the concentration of the ascorbic acid is 20-200mg/L, two optium concentrations of the ascorbic acid in the Chinese cabbage seed initiator are respectively 50 and 80mg/L, the buffer is one of glucose, fumaric acid or a variety of, and the fungicide is tetramethyl thiuram disulfide.The invention also discloses the application methods of the Chinese cabbage seed initiator.Chinese cabbage seed initiator of the present invention permeates initiation by ascorbic acid, control seed cell water suction, be conducive to improve Seed germinating ability, Chinese cabbage seed is promoted to sprout, have the function of the integrality for defending Lipid peroxidation metabolism and removing free radical, maintaining cell membrane, is more advantageous to growth of seedling.

Description

A kind of Chinese cabbage seed initiator and its application method
Technical field
The present invention relates to Seed priming techniques field, specifically a kind of Chinese cabbage seed initiator and its application side Method.
Background technique
Seed priming (Seed priming) is so that seed is slowly absorbed water under controlled conditions, to carry out in advance to sprout One kind that physiology prepares broadcasts preceding seed processing technology, is a kind of effective way for improving seed vitality.Seed priming techniques exist It is applied successfully in the various plants type such as vegetable crop, crops, industrial crops, ornamental plant, forest, including such as eggplant All kinds of vegetables such as son, capsicum, tomato, cucumber, muskmelon, muskmelon, watermelon, cabbage, carrot, celery, spinach, shallot, onion Dish；The plurality of cereals crop such as barley, wheat, corn, rice and sorghum；And dustymiller, Verbena, Petunia and three colors A variety of ornamental plants such as violet.Good germination effect is obtained by initiation processing, the neat rate and resistance of seedling is improved, is Its later period growth and development establishes good basis.
Chinese cabbage (Brassica campestris ssp.chinensis Makino) belongs to Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies, are commonly called as green vegetables, pakchoi, rape etc., are the important vegetables for originating in China.Not balling Chinese cabbage quality is fresh and tender, full of nutrition, is favored by people, and occupies an important position in China's vegetable consumption.Chinese cabbage is more It is cultivated using direct-seeding, seed grain is small, there are the actual productions such as application rate is big, emergence rate is lower, Seedling Quality is poor, Therefore, it improves seed vitality and Seedling Quality all has a direct impact effect to the yield and quality of Chinese cabbage.Research is not tied Ball Chinese cabbage seed causes initiator used and mostly uses potassium nitrate, sodium chloride, calcium chloride and Macrogol 6000 etc..
Ascorbic acid (L-Ascorbic acid) is that plant is important to one of the non-Enzyme-recovery-system of Lipid peroxidation metabolism Substance has stronger reducing power, has defence Lipid peroxidation metabolism and removes the function of free radical, the integrality for maintaining cell membrane Energy.Existing research shows that Exogenous Ascorbic Acid causes the Germinating status that can significantly improve the rape, soya seeds coerced, energy Significantly improve the germinating power of maca aged seed.For the treatment effect of Chinese cabbage seed, there is not been reported for it.
Summary of the invention
The purpose of the present invention is to provide a kind of Chinese cabbage seed initiator and its application methods, to solve above-mentioned back The problem of being proposed in scape technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
As a further solution of the present invention: the concentration of the ascorbic acid is 20-200mg/L.
As further scheme of the invention: the concentration of the ascorbic acid is 50mg/L.
As further scheme of the invention: the concentration of the ascorbic acid is 80mg/L.
As the present invention further scheme: the buffer is one of glucose, fumaric acid or a variety of.
As further scheme of the invention: the fungicide is tetramethyl thiuram disulfide.
The application method of the Chinese cabbage seed initiator, by the Chinese cabbage seed initiator of each concentration and 500 Chinese cabbage seeds are placed in triangular flask, and initiation is handled for 24 hours under 20 DEG C of dark conditions.
Compared with prior art, the beneficial effects of the present invention are:
1. Chinese cabbage seed initiator of the present invention permeates initiation by ascorbic acid, control seed cell is inhaled Water is conducive to improve Seed germinating ability, Chinese cabbage seed is promoted to sprout.
2. Chinese cabbage seed initiator of the present invention has defence Lipid peroxidation metabolism and removes free radical, maintains cell membrane Integrality function, be more advantageous to growth of seedling.
3. the present invention is easily operated.
4. Chinese cabbage seed initiator of the present invention has the advantages that natural environmental-protective, degradable, nontoxic.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment 1
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
Wherein, the concentration of the ascorbic acid is 20mg/L, and the buffer is glucose, and the fungicide is tetramethyl Thiuram disulfides.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
Embodiment 2
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
Wherein, the concentration of the ascorbic acid is 50mg/L, and the buffer is fumaric acid, and the fungicide is tetramethyl Base thiuram disulfides.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
Embodiment 3
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
Wherein, the concentration of the ascorbic acid is 80mg/L, and the buffer is glucose, and the fungicide is tetramethyl Thiuram disulfides.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
Embodiment 4
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
Wherein, the concentration of the ascorbic acid is 100mg/L, and the buffer is fumaric acid, and the fungicide is four Methyl thiuram disulfides.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
Embodiment 5
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
Wherein, the concentration of the ascorbic acid is 150mg/L, and the buffer is the combination of glucose and fumaric acid Object, and the ratio of glucose and fumaric acid is 1:1, the fungicide is tetramethyl thiuram disulfide.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
Embodiment 6
A kind of Chinese cabbage seed initiator, comprising: ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, Fungicide.
Wherein, the concentration of the ascorbic acid is 200mg/L, and the buffer is glucose, and the fungicide is tetramethyl Base thiuram disulfides.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
Comparative example
The Chinese cabbage seed initiator of this comparative example includes: distilled water, calcium chloride, gibberellin, polyethylene glycol, buffering Agent, fungicide.
Wherein, the buffer is glucose, and the fungicide is tetramethyl thiuram disulfide.
The application method of the Chinese cabbage seed initiator selects full, neat and consistent seed, is placed in 150ml Triangular flask in, 500 seeds are put into each triangular flask, under 20 DEG C of dark conditions initiation processing for 24 hours.
1, seed germination experiment:
Material to be tested used in this test be Chinese cabbage kind ' green basket ' (2013 harvesting and 2017 harvest), ' heat is green ' (harvesting in 2013 and harvesting in 2017) seed.
After handling according to the method for embodiment 1-6 and comparative example, 2min is washed with deionized water in seed, it will under the conditions of 25 DEG C Seed dries, and then seed is placed in two layers of filter paper, and injected clear water reaches in the 9cm culture dish of saturation, distance between seed It is put by 1-2 times of seed size.Each culture dish is put into 100 seeds, 3 different cultivars, different year, various concentration weights It is multiple.It is placed on 22 DEG C, 16h/17 DEG C, carries out germination test in the incubator under the dark condition of 8h temperature cycle.
2, germinate index determining
Measure germinating energy, 4 germination percentage, germination index and vitality index germination indexs of seed.Each index calculating method It is as follows:
Subnumber * 100% is planted experimentally in potentiality of seed (%)=initial stage (the 5th day) chitting piece number/confession；
Subnumber * 100% is planted experimentally in percentage of seedgermination (%)=final period (the 7th day) chitting piece number/confession；
Germination index (GI)=∑ Gt/Dt；
Vitality index (VI)=∑ Gt/Dt*S；
Wherein Gt is the chitting piece number of different germination days (counting 7d), and Dt is corresponding germination number of days, and S is corresponding germination day The total length of radicle and plumular axis when number.
3, result and analysis
By according to the method for embodiment 1-6 and comparative example treated Chinese cabbage ' green basket ' seed, referred to according in 1 Method carry out germination test, and by experimental result record as shown in table 1, table 2.
1 different disposal method of table (harvests) influence of germination for 2013 to Chinese cabbage ' green basket ' seed
Note: there is significant difference in 0.05 level between letter expression processing different in column data；Similarly hereinafter.
2 different disposal method of table (harvests) influence of germination for 2017 to Chinese cabbage ' green basket ' seed
By table 1, table 2 it is found that under embodiment processing, ' germinating energy, germination percentage, germination index and the vigor of green basket ' seed The variation of index is substantially in first to increase the trend reduced afterwards.Embodiment 2 is remarkably improved the germinating energy, germination index and work of seed Power index, compared with comparative example, ' germinating energy, germination index and the vitality index of green basket ' seed are respectively increased within 2013 4.16%, 3.72,127.56, ' green basket ' seed has then been respectively increased 9.17%, 9.36,20.35 within 2017.Embodiment processing pair Its germinating energy does not make a significant impact, but compares comparative example and obtain a certain range of raising.
By according to the method for embodiment 1-6 and comparative example treated Chinese cabbage ' heat is green ' seed, referred to according in 1 Method carry out germination test, and by experimental result record as shown in table 3, table 4.
3 different disposal method of table (harvests) influence of germination for 2013 to Chinese cabbage ' heat is green ' seed
4 different disposal method of table (harvests) influence of germination for 2017 to Chinese cabbage ' heat is green ' seed
Table 3 is the results show that in addition to the vitality index of embodiment 4,5 seeds decreases, other each embodiments are to ' heat It is green ' germinating energy, germination percentage, germination index and the vitality index of seed have different degrees of raising effect.The hair of embodiment 2-5 Bud rate has reached 100%.Compared to comparative example, the germinating energy and germination percentage of embodiment 2,3 are significantly improved, and embodiment 3 significantly increase germination characteristic effect it is best, the germinating energy of seed, germination percentage, germination index, vitality index are respectively increased 4.44%, 0.83%, 3.73,112.01.
As shown in Table 4, the germinating energy, germination percentage, germination index of each embodiment and the vitality index of embodiment 1,2 obtain Certain raising.The effect that embodiment 2 significantly improves the germinating energy of seed, germination percentage, germination index and vitality index is best, 8.61%, 5.83%, 5.06,22.37 are respectively increased compared to comparative example.
In conclusion the present invention provides a kind of Chinese cabbage seed initiator, the Chinese cabbage seed initiator Germinating energy, germination percentage, germination index and the vitality index for improving Chinese cabbage seed, significantly increase seed vitality.This is not Two optium concentrations of the ascorbic acid in Chinese cabbage seed initiator are respectively 50 and 80mg/L, can significantly improve seed Germinating power.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective It makes a variety of changes.

Claims (7)

1. a kind of Chinese cabbage seed initiator characterized by comprising ascorbic acid, calcium chloride, gibberellin, poly- second two Alcohol, buffer, fungicide.
2. Chinese cabbage seed initiator according to claim 1, which is characterized in that the concentration of the ascorbic acid is 20-200mg/L。
3. Chinese cabbage seed initiator according to claim 2, which is characterized in that the concentration of the ascorbic acid is 50mg/L。
4. Chinese cabbage seed initiator according to claim 2, which is characterized in that the concentration of the ascorbic acid is 80mg/L。
5. Chinese cabbage seed initiator according to claim 1, which is characterized in that the buffer be glucose, One of fumaric acid is a variety of.
6. Chinese cabbage seed initiator according to claim 1, which is characterized in that the fungicide is the tetramethyl autumn Blue nurse disulphide.
7. a kind of application method of the Chinese cabbage seed initiator as described in claim 1-6 is any, which is characterized in that will The Chinese cabbage seed initiator of each concentration and 500 Chinese cabbage seeds are placed in triangular flask, in 20 DEG C of dark conditions Lower initiation processing is for 24 hours.
CN201910167111.4A 2019-03-06 2019-03-06 A kind of Chinese cabbage seed initiator and its application method Pending CN109757489A (en)

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CN201910167111.4A CN109757489A (en) 2019-03-06 2019-03-06 A kind of Chinese cabbage seed initiator and its application method
PCT/CN2019/125941 WO2020177442A1 (en) 2019-03-06 2019-12-17 Brassica campestris ssp. chinensis makino seed priming agent and application method therefor

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
CN110301182A (en) * 2019-05-29 2019-10-08 金华市艾力生物科技有限公司 Preparation method of seed initiator
CN111386788A (en) * 2020-04-30 2020-07-10 南京农业大学 Initiator with disease resistance and insect resistance for small-sized seeds of non-heading Chinese cabbages and application of initiator
CN111466395A (en) * 2020-04-30 2020-07-31 南京农业大学 Natural environment-friendly non-heading Chinese cabbage seed coating agent and preparation method thereof
WO2020177442A1 (en) * 2019-03-06 2020-09-10 南京农业大学 Brassica campestris ssp. chinensis makino seed priming agent and application method therefor
CN113079746A (en) * 2021-05-19 2021-07-09 南京农业大学 Pelleting formula and processing method of seeds of brassica rapa pekinensis

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WO2023150508A2 (en) * 2022-02-01 2023-08-10 Zero Gravity Solutions, Inc. Plant priming compositions and methods of use thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020177442A1 (en) * 2019-03-06 2020-09-10 南京农业大学 Brassica campestris ssp. chinensis makino seed priming agent and application method therefor
CN110301182A (en) * 2019-05-29 2019-10-08 金华市艾力生物科技有限公司 Preparation method of seed initiator
CN110301182B (en) * 2019-05-29 2021-05-25 金华市艾力生物科技有限公司 Preparation method of seed initiator
CN111386788A (en) * 2020-04-30 2020-07-10 南京农业大学 Initiator with disease resistance and insect resistance for small-sized seeds of non-heading Chinese cabbages and application of initiator
CN111466395A (en) * 2020-04-30 2020-07-31 南京农业大学 Natural environment-friendly non-heading Chinese cabbage seed coating agent and preparation method thereof
CN111466395B (en) * 2020-04-30 2021-07-27 南京农业大学 Natural environment-friendly non-heading Chinese cabbage seed coating agent and preparation method thereof
CN111386788B (en) * 2020-04-30 2021-09-14 南京农业大学 Initiator with disease resistance and insect resistance for small-sized seeds of non-heading Chinese cabbages and application of initiator
CN113079746A (en) * 2021-05-19 2021-07-09 南京农业大学 Pelleting formula and processing method of seeds of brassica rapa pekinensis

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