CN111454915B - 一种h9亚型禽流感病毒分离鉴定方法 - Google Patents
一种h9亚型禽流感病毒分离鉴定方法 Download PDFInfo
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16151—Methods of production or purification of viral material
Abstract
Description
稀释度 | 10-8 | 10-9 | 10-10 | 10-11 |
HA效价 | 9log2 | 9log2 | 8log2 | 0 |
阳性结果判定 | 阳性 | 阳性 | 阳性 | 阴性 |
接种对象 | HA效价不低于4log2样品 |
MDCK细胞 | 12/200 |
SPF鸡胚 | 10/200 |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101816785A (zh) * | 2010-04-29 | 2010-09-01 | 扬州优邦生物制药有限公司 | 一种h9n2亚型禽流感灭活疫苗的制备方法及产品 |
CN101979517A (zh) * | 2010-10-15 | 2011-02-23 | 洛阳普莱柯生物工程有限公司 | 一种利用生物反应器大规模生产流感病毒的方法 |
CN102600464A (zh) * | 2012-03-08 | 2012-07-25 | 扬州威克生物工程有限公司 | 禽流感病毒灭活疫苗的制备方法及产品 |
CN104001167A (zh) * | 2014-05-19 | 2014-08-27 | 郑州爱科生物科技有限公司 | 全悬浮培养细胞制造禽流感灭活疫苗工艺及产品 |
CN104073470A (zh) * | 2014-06-30 | 2014-10-01 | 中国农业科学院上海兽医研究所 | 一种h9n2亚型禽流感病毒的转瓶培养方法 |
CN104338127A (zh) * | 2014-10-09 | 2015-02-11 | 江苏省农业科学院 | 一种h9n2亚型禽流感灭活疫苗的生产方法及其制品 |
CN110237246A (zh) * | 2019-07-25 | 2019-09-17 | 北京鼎持生物技术有限公司 | 一种全悬浮细胞培养禽流感(h9)灭活疫苗的方法 |
-
2020
- 2020-03-10 CN CN202010164450.XA patent/CN111454915B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101816785A (zh) * | 2010-04-29 | 2010-09-01 | 扬州优邦生物制药有限公司 | 一种h9n2亚型禽流感灭活疫苗的制备方法及产品 |
CN101979517A (zh) * | 2010-10-15 | 2011-02-23 | 洛阳普莱柯生物工程有限公司 | 一种利用生物反应器大规模生产流感病毒的方法 |
CN102600464A (zh) * | 2012-03-08 | 2012-07-25 | 扬州威克生物工程有限公司 | 禽流感病毒灭活疫苗的制备方法及产品 |
CN104001167A (zh) * | 2014-05-19 | 2014-08-27 | 郑州爱科生物科技有限公司 | 全悬浮培养细胞制造禽流感灭活疫苗工艺及产品 |
CN104073470A (zh) * | 2014-06-30 | 2014-10-01 | 中国农业科学院上海兽医研究所 | 一种h9n2亚型禽流感病毒的转瓶培养方法 |
CN104338127A (zh) * | 2014-10-09 | 2015-02-11 | 江苏省农业科学院 | 一种h9n2亚型禽流感灭活疫苗的生产方法及其制品 |
CN110237246A (zh) * | 2019-07-25 | 2019-09-17 | 北京鼎持生物技术有限公司 | 一种全悬浮细胞培养禽流感(h9)灭活疫苗的方法 |
Non-Patent Citations (3)
Title |
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史爱华 ; 姜北宇 ; 沈佳 ; 景小冬 ; 章振华 ; 李林 ; 张建伟 ; .胰蛋白酶浓度对H9亚型禽流感病毒在MDCK细胞上增殖的影响.安徽农业科学.2011,39(35),21813 - 21815. * |
史爱华 ; 张建伟 ; 沈佳 ; 姜北宇 ; 章振华 ; 李林 ; 景小冬 ; .H9N2亚型禽流感病毒在MDCK细胞中增殖最佳条件研究.动物医学进展.2011,32(11),42-45. * |
吴亚丽 ; 宋玉慧 ; 徐倩 ; 潘梦梦 ; 张剑 ; 武志丹 ; 李新生 ; .适应H9亚型禽流感病毒增殖的重组R-MDCK细胞系的建立.中国兽医学报.2016,36(4),579-584. * |
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