CN111454915A - 一种h9亚型禽流感病毒分离鉴定方法 - Google Patents
一种h9亚型禽流感病毒分离鉴定方法 Download PDFInfo
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Abstract
Description
稀释度 | 10<sup>-8</sup> | 10<sup>-9</sup> | 10<sup>-10</sup> | 10<sup>-11</sup> |
HA效价 | 9log2 | 9log2 | 8log2 | 0 |
阳性结果判定 | 阳性 | 阳性 | 阳性 | 阴性 |
接种对象 | HA效价不低于4log2样品 |
MDCK细胞 | 12/200 |
SPF鸡胚 | 10/200 |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101816785A (zh) * | 2010-04-29 | 2010-09-01 | 扬州优邦生物制药有限公司 | 一种h9n2亚型禽流感灭活疫苗的制备方法及产品 |
CN101979517A (zh) * | 2010-10-15 | 2011-02-23 | 洛阳普莱柯生物工程有限公司 | 一种利用生物反应器大规模生产流感病毒的方法 |
CN102600464A (zh) * | 2012-03-08 | 2012-07-25 | 扬州威克生物工程有限公司 | 禽流感病毒灭活疫苗的制备方法及产品 |
CN104001167A (zh) * | 2014-05-19 | 2014-08-27 | 郑州爱科生物科技有限公司 | 全悬浮培养细胞制造禽流感灭活疫苗工艺及产品 |
CN104073470A (zh) * | 2014-06-30 | 2014-10-01 | 中国农业科学院上海兽医研究所 | 一种h9n2亚型禽流感病毒的转瓶培养方法 |
CN104338127A (zh) * | 2014-10-09 | 2015-02-11 | 江苏省农业科学院 | 一种h9n2亚型禽流感灭活疫苗的生产方法及其制品 |
CN110237246A (zh) * | 2019-07-25 | 2019-09-17 | 北京鼎持生物技术有限公司 | 一种全悬浮细胞培养禽流感(h9)灭活疫苗的方法 |
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2020
- 2020-03-10 CN CN202010164450.XA patent/CN111454915B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101816785A (zh) * | 2010-04-29 | 2010-09-01 | 扬州优邦生物制药有限公司 | 一种h9n2亚型禽流感灭活疫苗的制备方法及产品 |
CN101979517A (zh) * | 2010-10-15 | 2011-02-23 | 洛阳普莱柯生物工程有限公司 | 一种利用生物反应器大规模生产流感病毒的方法 |
CN102600464A (zh) * | 2012-03-08 | 2012-07-25 | 扬州威克生物工程有限公司 | 禽流感病毒灭活疫苗的制备方法及产品 |
CN104001167A (zh) * | 2014-05-19 | 2014-08-27 | 郑州爱科生物科技有限公司 | 全悬浮培养细胞制造禽流感灭活疫苗工艺及产品 |
CN104073470A (zh) * | 2014-06-30 | 2014-10-01 | 中国农业科学院上海兽医研究所 | 一种h9n2亚型禽流感病毒的转瓶培养方法 |
CN104338127A (zh) * | 2014-10-09 | 2015-02-11 | 江苏省农业科学院 | 一种h9n2亚型禽流感灭活疫苗的生产方法及其制品 |
CN110237246A (zh) * | 2019-07-25 | 2019-09-17 | 北京鼎持生物技术有限公司 | 一种全悬浮细胞培养禽流感(h9)灭活疫苗的方法 |
Non-Patent Citations (3)
Title |
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史爱华;姜北宇;沈佳;景小冬;章振华;李林;张建伟;: "胰蛋白酶浓度对H9亚型禽流感病毒在MDCK细胞上增殖的影响" * |
史爱华;张建伟;沈佳;姜北宇;章振华;李林;景小冬;: "H9N2亚型禽流感病毒在MDCK细胞中增殖最佳条件研究" * |
吴亚丽;宋玉慧;徐倩;潘梦梦;张剑;武志丹;李新生;: "适应H9亚型禽流感病毒增殖的重组R-MDCK细胞系的建立" * |
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