CN111407692A - Sodium hyaluronate stock solution containing plant bacteriostatic components and preparation method thereof - Google Patents
Sodium hyaluronate stock solution containing plant bacteriostatic components and preparation method thereof Download PDFInfo
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- CN111407692A CN111407692A CN201910007511.9A CN201910007511A CN111407692A CN 111407692 A CN111407692 A CN 111407692A CN 201910007511 A CN201910007511 A CN 201910007511A CN 111407692 A CN111407692 A CN 111407692A
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- sodium hyaluronate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The invention relates to a sodium hyaluronate stock solution containing plant bacteriostatic components and a preparation method thereof, and the sodium hyaluronate stock solution is characterized in that: the paint comprises the following components in percentage by weight: 0.1-3% of plant bacteriostatic mixture, 0.02-2% of sodium hyaluronate, 0.1-10% of humectant and 0.001-1% of pH regulator. The plant bacteriostatic compound is an extract obtained by mixing pistachio, folium artemisiae argyi, oldenlandia diffusa, honeysuckle and scutellaria baicalensis, and the weight ratio of the pistachio, the folium artemisiae argyi, the oldenlandia diffusa, the honeysuckle and the scutellaria baicalensis is as follows: 30: 20: 10: 10: 30. the sodium hyaluronate stock solution is colorless transparent to yellowish clear liquid, does not contain preservative, has good safety and stability, has a moisturizing effect on skin, has no sensitization on the skin, improves the elasticity of the skin, tightens the skin and plays a role in rejuvenating the skin.
Description
Technical Field
The invention relates to the field of cosmetic application, in particular to a sodium hyaluronate stock solution containing plant bacteriostatic components and a preparation method thereof.
Background
The sodium hyaluronate is an acidic mucopolysaccharide naturally existing in a human body, has different physiological activities according to different molecular sizes, can form an invisible breathable film when acting on the surface of the skin, is used for adjusting the relative humidity of the surface of the skin, provides a wet environment suitable for the skin, can penetrate through a epidermal layer and permeate into a superficial dermis to play a role in supplementing water, can remove free radicals, promotes the synthesis of collagen and elastin, and plays a role in resisting aging. At present, a great number of sodium hyaluronate stock solutions are available on the market, however, the necessary condition for commercialization is shelf life, and the preservative therein is a key ingredient for ensuring the quality of cosmetics and the safety of consumers. The chemical preservative has high preservative effect and small dosage, is widely applied in the field of pharmacy, but is worthy of attention, the application course in the field of pharmacy is short, the period is long, the preservative can be applied only when diseases appear, and skin care products are daily products which need to be used every day, so the influence of the preservative is particularly important, data reports show that the incidence rate of skin or heavy or light anaphylactic reaction is 60-70%, most of patients are caused by improper use of the skin care products, and the preservative is an important factor, so that it is necessary to find a safe and plant-derived natural material with bacteriostatic action, and the inventor finds that 5 plant mixtures are extracted according to a certain proportion: the obtained extract has the function of antisepsis, can be used in cosmetics, can pass antiseptic challenge experiments, and has no adverse reaction in the using process. The sodium hyaluronate stock solution prepared by the antibacterial mixture does not contain a preservative, has good safety and stability, has a moisturizing effect on skin, has no sensitization on the skin, improves the skin elasticity, tightens the skin and plays a role in rejuvenating the skin.
Disclosure of Invention
The invention aims to solve the technical problem of providing a sodium hyaluronate stock solution containing plant bacteriostatic components, which does not contain the traditional chemical preservative, and has good moisturizing effect and better safety and stability.
Specifically, the invention comprises a sodium hyaluronate stock solution containing plant bacteriostatic components, which is characterized in that: the paint comprises the following components in percentage by weight:
84 to 99.77 percent of purified water
0.1-3% of plant bacteriostatic mixture
0.02-2% of sodium hyaluronate
0.1 to 10 percent of humectant
0.001-1% of pH regulator.
Wherein the humectant can be one or more of glycerol, butanediol, pentanediol, lactic acid, sodium lactate, betaine, trehalose, erythritol, Cucumis sativus extract, cyclobalanopsis glauca extract, Aloe extract, glucose, glycerol glucoside, mannitol, sorbitol, cranberry extract, hydrolyzed sodium hyaluronate, revived grass extract, and herba Portulacae extract.
The pH regulator can be one or more of sodium hydroxide, potassium hydroxide, hydrochloric acid, acetic acid, lactic acid, citric acid, sodium citrate, aminomethyl propanol, and arginine.
More specifically, the sodium hyaluronate stock solution comprises the following components in percentage by weight:
92.7 percent of purified water
0.3 percent of plant bacteriostatic mixture
0.5 percent of sodium hyaluronate
1.0 percent of hydrolyzed sodium hyaluronate
1% of glycerol
4 percent of butanediol
Glycerol glucoside 0.5%
Cucumis sativus extract 4%
0.005 percent of citric acid
The plant bacteriostatic mixture is prepared by the following method:
1) cleaning pistachio, folium artemisiae argyi, oldenlandia diffusa, honeysuckle and scutellaria baicalensis, drying in the air, and mixing the components in a weight ratio of 30: 20: 10: 10: 30, and crushing.
2) According to the weight percentage of water: ethanol weight ratio 2: 8, adding citric acid-sodium citrate buffer pair, and adjusting the pH value to 5.2-6.5 to be used as an extraction solvent.
3) And pouring the crushed mixture into an extraction solvent, wherein the extraction proportion is 1 part of dry powder mixture and 4 parts of extract, soaking for 8 hours at normal temperature, and then refluxing for 2 hours at 70-80 ℃.
4) And after the leaching is finished, cooling to room temperature, filtering through 100-mesh filter cloth, then filtering through 200-mesh filter cloth, performing rotary evaporation on the obtained filtrate, and concentrating to dryness to obtain the plant bacteriostatic mixture.
Furthermore, the preparation method of the sodium hyaluronate stock solution comprises the following steps: dispersing the mixture of sodium hyaluronate and plant bacteriostasis into a humectant, pouring the mixture into purified water for dissolving at a temperature not exceeding 60 ℃ after uniform dispersion, adding other humectants and pH regulators once after complete dissolution, and adding water to a specified weight to obtain colorless or yellowish clear liquid.
Detailed Description
The invention will be further illustrated with reference to specific embodiments:
examples, see table 1.
TABLE 1 formulation of examples of the invention
| Material name/%) | Example 1 | Example 2 | Example 3 | Example 4 |
| Water (W) | 88.7 | 93.9 | 88.9 | 94.9 |
| Hyaluronic acid sodium salt | 0.5 | 2 | 0.6 | 0.3 |
| Hydrolyzed sodium hyaluronate | 1 | 0.1 | 0.5 | 0.5 |
| Plant bacteriostatic mixture | 0.3 | 0.5 | 2 | 0.1 |
| Glycerol | 1 | -- | 2 | -- |
| Butanediol | 4 | 2 | 4 | -- |
| Pentanediol | -- | -- | -- | 2 |
| Glycerol glucoside | 0.5 | 1 | -- | 0.3 |
| Cucumis sativus extract | 4 | -- | -- | -- |
| Extracts of revival grass | -- | 0.5 | -- | 2 |
| Erythritol and its preparation method | -- | -- | 2 | 1 |
| Citric acid | 0.005 | -- | -- | -- |
| Arginine | -- | -- | 0.1 | -- |
The preparation method comprises the following steps: adding the mixture of sodium hyaluronate and plant bacteriostatic into alcohol product, dispersing uniformly, pouring into purified water, stirring to dissolve, sequentially adding other materials in the formula, adding water to a sufficient amount, and stirring uniformly.
To verify the results of the present invention, the following experimental verification was performed.
1. Experiment of corrosion prevention challenge
The samples of examples 1-4 were subjected to preservative challenge experiments according to the "cosmetic preservative efficacy evaluation method operating guidelines" published by the national institute for food and drug testing, and the results are shown in table 2.
Table 2 results of preservative challenge experiments for sodium hyaluronate solutions containing plant bacteriostatic components
According to the experimental results, the total number of the bacterial colonies of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa is reduced by 99.9% when the staphylococcus aureus, the escherichia coli and the pseudomonas aeruginosa are filled up at 7 th, the bacterial colonies are not increased at 28 th, the total number of the aspergillus niger and the candida albicans is reduced by 99.0% at 14 th, and the total number of the aspergillus niger and the candida albicans is not increased at 28 th, which indicates that the plant bacteriostatic mixture has a good preservative effect.
2. Measurement of moisturizing Effect and Observation of allergy Rate
The experimental method comprises the steps of selecting 80 women with the ages of 20-40, randomly dividing the women into 4 groups, coating 20 persons in each group by taking cheeks as test parts according to the standard of 1.5ml of faces in the moisture retention performance test by using sodium hyaluronate stock solution in examples 1-4, and performing skin moisture content test on the cheeks of 20 subjects in each group by using a Corneometer CM820 skin moisture tester in an indoor environment with the relative humidity of 20 ℃ and 50%, wherein the result is represented by a measured humidity value MMV, MMV1 is the MMV value of the skin before coating the product, MMV2 is the MMV value of the skin after 30 minutes of coating the product, MMV3 is the MMV value of the skin after 3 hours of coating the product, the water supplement rate = MMV2/MMV1 × 100%, and the MMV rate = 3/1 × 100%, and simultaneously observing whether the subjects are sensitive to fever, redness, itching and the like, and the experimental result shows the surface symptoms of 3.
TABLE 3 moisturizing effect and sensitivity measurement results of sodium hyaluronate solution containing plant bacteriostatic component
| Group of | The water replenishing rate% | Moisture retention rate% | Sensitivity ratio% |
| Example 1 | 233 | 140 | 0 |
| Example 2 | 251 | 156 | 0 |
| Example 3 | 266 | 160 | 0 |
| Example 4 | 280 | 139 | 0 |
The experimental results show that the water replenishing rate and the moisture retention rate of the sodium hyaluronate stock solution prepared in the examples 1 to 4 are remarkably improved after the sodium hyaluronate stock solution is applied to the skin, and the sodium hyaluronate solution containing plant bacteriostatic components has good water replenishing and moisture retention effects on the skin and has no sensitivity.
3. Stability determination
The samples of examples 1 to 4 were subjected to stability test, including cold resistance test, heat resistance test, and accelerated test, respectively.
The cold resistance test method comprises the following steps: and (4) putting the completely packaged sample into a constant temperature box for 24 hours at the temperature of between 5 ℃ below zero and 10 ℃ below zero, recovering to the room temperature, and observing the performance index.
The heat resistance test method comprises the following steps: and (3) putting the completely packaged sample into a constant temperature box (40 +/-1) DEG C, keeping the temperature for 24h, recovering to the room temperature, and observing performance indexes.
The accelerated test method comprises the following steps: and (3) putting the completely packaged sample into a constant temperature box (40 +/-1) DEG C, keeping for 3 months, sampling at 0 month, 1 month and 3 months respectively, recovering to the room temperature, and observing performance indexes.
The results are shown in Table 4.
Table 4 stability test results of sodium hyaluronate solution containing plant bacteriostatic components
From the above results, the hyaluronic acid stock solution containing the plant bacteriostatic mixture has stable appearance and pH value through cold resistance, heat resistance and accelerated experiments.
According to the experimental results, the plant bacteriostatic mixture has good antiseptic effect, excellent moisturizing and water replenishing effects, no anaphylactic reaction and good stability in the sodium hyaluronate solution.
Although the present invention has been described with reference to the above embodiments, it should be understood that the scope of the present invention is not limited thereto, and any changes and modifications made by those skilled in the art without departing from the spirit and scope of the present invention should be construed as being limited thereto.
Claims (4)
1. A sodium hyaluronate stock solution containing plant bacteriostatic components is characterized in that: the paint comprises the following components in percentage by weight:
84-99.77% of purified water, 0.1-3% of plant bacteriostatic mixture, 0.02-2% of sodium hyaluronate, 0.1-10% of humectant and 0.001-1% of pH value regulator, wherein the humectant can be one or more of glycerol, butanediol, pentanediol, lactic acid, sodium lactate, betaine, trehalose, erythritol, gherkin extract, cyclobalanopsis glauca extract, aloe extract, glucose, glycerol glucoside, mannitol, sorbitol, cranberry extract, hydrolyzed sodium hyaluronate, revived grass extract and purslane extract, and the pH value regulator can be one or more of sodium hydroxide, potassium hydroxide, hydrochloric acid, acetic acid, lactic acid, citric acid, sodium citrate, aminomethyl propanol and arginine.
2. The sodium hyaluronate stock solution containing plant bacteriostatic components according to claim 1, which is characterized in that: the paint comprises the following components in percentage by weight: 92.7% of purified water, 0.3% of plant bacteriostatic mixture, 0.5% of sodium hyaluronate, 1.0% of hydrolyzed sodium hyaluronate, 1% of glycerol, 4% of butanediol, 0.5% of glycerol glucoside, 4% of cucumber extract and 0.005% of citric acid.
3. The sodium hyaluronate stock solution containing plant bacteriostatic components according to the claims 1-2, wherein the plant bacteriostatic mixture is prepared by the following method: cleaning pistachio, folium artemisiae argyi, oldenlandia diffusa, honeysuckle and scutellaria baicalensis, drying in the air, and mixing the components in a weight ratio of 30: 20: 10: 10: 30, and crushing; according to the weight percentage of water: ethanol weight ratio 2: 8, adding a citric acid-sodium citrate buffer pair, and adjusting the pH value to 5.2-6.5 to be used as an extraction solvent; pouring the crushed mixture into an extraction solvent, wherein the extraction proportion is 1 part of dry powder mixture and 4 parts of extract, soaking at normal temperature for 8 hours, and then refluxing at 70-80 ℃ for 2 hours; and after the leaching is finished, cooling to room temperature, filtering through 100-mesh filter cloth, then filtering through 200-mesh filter cloth, performing rotary evaporation on the obtained filtrate, and concentrating to dryness to obtain the plant bacteriostatic mixture.
4. The sodium hyaluronate stock solution containing plant bacteriostatic components as claimed in claims 1-2, which is prepared by the following steps: dispersing the mixture of sodium hyaluronate and plant bacteriostasis into a humectant, after uniform dispersion, immersing into purified water for dissolution at a temperature not exceeding 60 ℃, after complete dissolution, adding other humectants and pH regulators at one time, and adding water to a specified weight to obtain colorless or yellowish clear liquid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910007511.9A CN111407692A (en) | 2019-01-04 | 2019-01-04 | Sodium hyaluronate stock solution containing plant bacteriostatic components and preparation method thereof |
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| CN201910007511.9A CN111407692A (en) | 2019-01-04 | 2019-01-04 | Sodium hyaluronate stock solution containing plant bacteriostatic components and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111759765A (en) * | 2020-07-16 | 2020-10-13 | 深圳博大精深企业管理咨询有限公司 | Water light substrate skin antibacterial liquid and preparation method thereof |
| CN112870149A (en) * | 2021-01-26 | 2021-06-01 | 广州华厦生物制药有限公司 | Vegetable protein repairing stock solution and preparation process thereof |
| CN112972310A (en) * | 2021-03-23 | 2021-06-18 | 青岛智博生物科技有限公司 | Composition with antibacterial and repairing effects and preparation method thereof |
-
2019
- 2019-01-04 CN CN201910007511.9A patent/CN111407692A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111759765A (en) * | 2020-07-16 | 2020-10-13 | 深圳博大精深企业管理咨询有限公司 | Water light substrate skin antibacterial liquid and preparation method thereof |
| CN112870149A (en) * | 2021-01-26 | 2021-06-01 | 广州华厦生物制药有限公司 | Vegetable protein repairing stock solution and preparation process thereof |
| CN112972310A (en) * | 2021-03-23 | 2021-06-18 | 青岛智博生物科技有限公司 | Composition with antibacterial and repairing effects and preparation method thereof |
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